Rh7CG198600

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0010311
Physical Location & Seq
Reverse (-)
17627692 .. 17629981
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG198600.1

Sequence Viewer

Length: 696 bp
ATGTTTGCACTTTTCAGGACAGGAAACCACAGATCATCACGTGCACAAGTTCGAATGATGCCAATAGGGACACCCAAGGTTCCCTATCGAACCCCTGGTGAGGGAGGTTGGCAATGGGTTGATCTGTGGAATGCTCTGTACCGGGAACGTGTTATCTTCATTGGGCAAAATATAGATGAAGAGTTTAGCAATCAAATTCTGGCAACAATGCTGTACCTTGACACAATTGATTCGTCCAAAAGGCTTTATATGTATATCAATGGTCCTGGTGGAGATCTTACTCCGAGCATGGCTATCTATGATACTATGCAAAGCTTAAAGAGTCCTGTTGGCACCCACTGTGTGGGCTATGCCTATAATTTGGCAGCCTTTCTTCTTGCAGCTGGAGAAAAGGGTAATCGATTTGCAATGCCACTATCAAGAATTGCACTGCAGTCTCCTGCTGGGGCTGCTCGTGGTCAGGCTGATGACATACGCAATGAGGCAAACGAGCTTCTCAGAATCAGAGATTACCTTTTTAATGAGTTGGCAGAGAAAACTGGCCAGCCAATTGAAAAGATTAACAAAGACCTAAGTCGGATGAAGCGCTTTAATGCACAGGAAGCTCTTGAGTATGGGCTCATTGATCGTGTAGTTAGGCCGCCTCGTATTAAGGCCGACTCACCTAGCAAAGATGCGGGAACAGGTCTTGGTTAG

Protein Analysis

231

Amino Acids

25.85

Weight (kDa)

9.14

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 37 - 213 4.6e-65 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 332
AccB7I CCANNNNNTGG 1 cut(s) 343
AciI CCGC 2 cut(s) 641, 677
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 1 cut(s) 195
AcvI CACGTG 1 cut(s) 41
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 2 cut(s) 140, 215
AfeI AGCGCT 1 cut(s) 587
AfiI CCNNNNNNNGG 3 cut(s) 100, 101, 343
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 1 cut(s) 554
AjnI CCWGG 2 cut(s) 94, 265
AluBI AGCT 4 cut(s) 315, 383, 493, 605
AluI AGCT 4 cut(s) 315, 383, 493, 605
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 441
Alw44I GTGCAC 1 cut(s) 42
Aor51HI AGCGCT 1 cut(s) 587
AoxI GGCC 3 cut(s) 541, 638, 654
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 3 cut(s) 365, 380, 449
ApoI RAATTY 1 cut(s) 195
AspLEI GCGC 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 110, 654
AsuII TTCGAA 1 cut(s) 52
AvaII GGWCC 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 621
BauI CACGAG 1 cut(s) 453
BbrPI CACGTG 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 3 cut(s) 377, 392, 436
BciT130I CCWGG 2 cut(s) 96, 267
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 1 cut(s) 666
BfmI CTRYAG 1 cut(s) 431
BfoI RGCGCY 1 cut(s) 589
BglII AGATCT 1 cut(s) 274
BisI GCNGC 4 cut(s) 366, 381, 450, 641
BlsI GCNGC 4 cut(s) 367, 382, 451, 642
Bme1390I CCNGG 3 cut(s) 96, 143, 267
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 81, 334
BmrFI CCNGG 3 cut(s) 96, 143, 267
BmsI GCATC 2 cut(s) 48, 664
BoxI GACNNNNGTC 1 cut(s) 573
BpmI CTGGAG 1 cut(s) 405
Bpu14I TTCGAA 1 cut(s) 52
BpuEI CTTGAG 1 cut(s) 629
BpuMI CCSGG 1 cut(s) 143
Bsa29I ATCGAT 1 cut(s) 400
BsaAI YACGTR 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 75, 94
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 101, 343
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 3 cut(s) 119, 414, 484
BseBI CCWGG 2 cut(s) 96, 267
BseCI ATCGAT 1 cut(s) 400
BseDI CCNNGG 2 cut(s) 75, 94
BseGI GGATG 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 100, 101, 343
BseMI GCAATG 3 cut(s) 119, 414, 484
BseMII CTCAG 1 cut(s) 511
BseNI ACTGG 1 cut(s) 544
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 3 cut(s) 377, 392, 436
BseYI CCCAGC 1 cut(s) 443
BshFI GGCC 3 cut(s) 543, 640, 656
BshNI GGYRCC 1 cut(s) 332
BshVI ATCGAT 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 3 cut(s) 100, 101, 343
BsmAI GTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 3 cut(s) 543, 640, 656
Bsp119I TTCGAA 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 46, 621
Bsp143I GATC 4 cut(s) 32, 121, 274, 625
BspACI CCGC 2 cut(s) 641, 677
BspANI GGCC 3 cut(s) 543, 640, 656
BspCNI CTCAG 1 cut(s) 510
BspDI ATCGAT 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 81, 334
BspMAI CTGCAG 1 cut(s) 435
BspT104I TTCGAA 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 332
BsrDI GCAATG 3 cut(s) 119, 414, 484
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 75, 94
BssMI GATC 4 cut(s) 32, 121, 274, 625
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 96, 267
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 41
BstBI TTCGAA 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 2 cut(s) 497, 572
BstF5I GGATG 1 cut(s) 585
BstH2I RGCGCY 1 cut(s) 589
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 4 cut(s) 35, 124, 277, 628
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 4 cut(s) 32, 121, 274, 625
BstMWI GCNNNNNNNGC 2 cut(s) 449, 602
BstNI CCWGG 2 cut(s) 96, 267
BstPAI GACNNNNGTC 1 cut(s) 573
BstSCI CCNGG 3 cut(s) 94, 141, 265
BstSFI CTRYAG 1 cut(s) 431
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 377, 392, 436
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 400
BsuRI GGCC 3 cut(s) 543, 640, 656
BsuTUI ATCGAT 1 cut(s) 400
BtsCI GGATG 1 cut(s) 585
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 2 cut(s) 337, 428
Cac8I GCNNGC 1 cut(s) 545
CfoI GCGC 1 cut(s) 588
Cfr13I GGNCC 1 cut(s) 263
ClaI ATCGAT 1 cut(s) 400
Csp6I GTAC 2 cut(s) 139, 214
CviAII CATG 1 cut(s) 289
CviQI GTAC 2 cut(s) 139, 214
DdeI CTNAG 2 cut(s) 497, 572
DpnI GATC 4 cut(s) 34, 123, 276, 627
DpnII GATC 4 cut(s) 32, 121, 274, 625
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 263
Eco47III AGCGCT 1 cut(s) 587
Eco72I CACGTG 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 94, 265
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 1 cut(s) 292
FaqI GGGAC 1 cut(s) 82
FatI CATG 1 cut(s) 288
FauI CCCGC 1 cut(s) 670
Fnu4HI GCNGC 4 cut(s) 366, 381, 450, 641
FokI GGATG 1 cut(s) 592
FriOI GRGCYC 1 cut(s) 621
Fsp4HI GCNGC 4 cut(s) 366, 381, 450, 641
FspBI CTAG 1 cut(s) 666
GlaI GCGC 1 cut(s) 587
GluI GCNGC 4 cut(s) 366, 381, 450, 641
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 589
HaeIII GGCC 3 cut(s) 543, 640, 656
HapII CCGG 1 cut(s) 142
HhaI GCGC 1 cut(s) 588
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 4 cut(s) 230, 322, 501, 659
HpaII CCGG 1 cut(s) 142
HphI GGTGA 2 cut(s) 110, 654
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 4 cut(s) 285, 500, 506, 579
Hpy188III TCNNGA 3 cut(s) 16, 420, 608
Hpy8I GTNNAC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 40, 148
HpyCH4V TGCA 8 cut(s) 8, 44, 310, 380, 407, 428, 433, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 602
HpyF3I CTNAG 2 cut(s) 497, 572
HpySE526I ACGT 2 cut(s) 40, 148
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 1 cut(s) 586
Kzo9I GATC 4 cut(s) 32, 121, 274, 625
Lsp1109I GCAGC 3 cut(s) 377, 392, 436
LweI GCATC 2 cut(s) 48, 664
MaeI CTAG 1 cut(s) 666
MaeII ACGT 2 cut(s) 40, 148
MalI GATC 4 cut(s) 34, 123, 276, 627
MboI GATC 4 cut(s) 32, 121, 274, 625
MboII GAAGA 3 cut(s) 148, 191, 365
MfeI CAATTG 2 cut(s) 225, 549
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 2 cut(s) 46, 621
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 5 cut(s) 195, 225, 358, 423, 549
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 2 cut(s) 331, 653
MmeI TCCRAC 1 cut(s) 557
MnlI CCTC 4 cut(s) 94, 98, 475, 654
Mox20I TGGCCA 1 cut(s) 543
MscI TGGCCA 1 cut(s) 543
MseI TTAA 5 cut(s) 317, 519, 561, 591, 651
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 3 cut(s) 96, 143, 267
MunI CAATTG 2 cut(s) 225, 549
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 2 cut(s) 96, 267
MwoI GCNNNNNNNGC 2 cut(s) 449, 602
NciI CCSGG 1 cut(s) 143
NdeII GATC 4 cut(s) 32, 121, 274, 625
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 2 cut(s) 81, 334
NspV TTCGAA 1 cut(s) 52
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 2 cut(s) 230, 501
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 4 cut(s) 367, 382, 451, 642
PleI GAGTC 2 cut(s) 330, 653
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
PpsI GAGTC 2 cut(s) 330, 653
Ppu21I YACGTR 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 573
Psp6I CCWGG 2 cut(s) 94, 265
PspCI CACGTG 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 2 cut(s) 94, 265
PspN4I GGNNCC 2 cut(s) 81, 334
PspPI GGNCC 1 cut(s) 263
PstI CTGCAG 1 cut(s) 435
PsuI RGATCY 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 140, 215
RsaNI GTAC 2 cut(s) 139, 214
SaqAI TTAA 5 cut(s) 317, 519, 561, 591, 651
SatI GCNGC 4 cut(s) 366, 381, 450, 641
Sau3AI GATC 4 cut(s) 32, 121, 274, 625
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 331, 653
ScrFI CCNGG 3 cut(s) 96, 143, 267
SduI GDGCHC 2 cut(s) 46, 621
SfaNI GCATC 2 cut(s) 48, 664
SfcI CTRYAG 1 cut(s) 431
SfuI TTCGAA 1 cut(s) 52
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 5 cut(s) 195, 225, 358, 423, 549
SsiI CCGC 2 cut(s) 641, 677
SspMI CTAG 1 cut(s) 666
StyD4I CCNGG 3 cut(s) 94, 141, 265
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 43, 151
TaqI TCGA 3 cut(s) 52, 88, 400
TasI AATT 5 cut(s) 195, 225, 358, 423, 549
TauI GCSGC 1 cut(s) 643
TfiI GAWTC 2 cut(s) 230, 501
Tru1I TTAA 5 cut(s) 317, 519, 561, 591, 651
Tru9I TTAA 5 cut(s) 317, 519, 561, 591, 651
TscAI CASTG 2 cut(s) 344, 435
TseI GCWGC 3 cut(s) 365, 380, 449
TspDTI ATGAA 3 cut(s) 148, 192, 596
TspRI CASTG 2 cut(s) 344, 435
Van91I CCANNNNNTGG 1 cut(s) 343
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 1 cut(s) 195
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.