MD06G1063900.v1.1

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010311
Physical Location & Seq
Reverse (-)
11053660 .. 11056796
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1063900.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGTTCTGTGCCAAAAATTAGCGACACAAAACCATAGGTCTTCACGAGCACGAGTTGGAATGATGCCTATAGGGACACCAAAAGTTCCCTACAGAACTCCTGGTGAGGGAACTTGGCAATGGGTTGATTTGTGGAATGCTCTATACCGAGAACGTGTGATCTTCATCGGGCAAAACATAGATGAAGAGTTCAGCAATCAAATTTTGGCAACAATGTTGTACCTTGACACTATAGATTCTTCGAAAAGGCTTTATATGTACATCAATGGTCCTGGTGGAGATCTTACTCCAAGCATGGCTATCTATGATACAATGCAGAGCCTAAAAAGTCCTGTTGGTACCCATTGTGTGGGCTATGCCTATAATTTAGCAGCCTTTCTTCTTACCGCTGGAGAGAAGGGAAATCGTTTTGCAATGCCACTATCAAGAATTGCTCTACAGTCTCCTGCCGGGGCTGCTCGTGGTCAGGCTGATGATATACAAAATGAAGCAAATGAACTTCTCAGAATTAGAGATTACCTGTTTAATGAGTTGGCTATGAAAACAGGCCAGCCAGTTGAAAAGATTAACAAAGACTTAAGTCGGATGAAGCGCTTTAATGCGCAGGAGGCTCTTGAATATGGGCTCATTGATCGTGTTGTTAGGCCACCCCGTATCAAGGCTGATGCACCTCCCAAGGACAGCGGATCAGGTCTTGGTTAG

Protein Analysis

234

Amino Acids

26.0

Weight (kDa)

9.02

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 40 - 215 4e-64 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 603
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 349
AciI CCGC 2 cut(s) 387, 684
AclWI GGATC 1 cut(s) 694
AcsI RAATTY 1 cut(s) 201
AfaI GTAC 3 cut(s) 221, 260, 340
AfeI AGCGCT 1 cut(s) 593
AfiI CCNNNNNNNGG 3 cut(s) 107, 349, 658
AflII CTTAAG 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 2 cut(s) 560, 617
AjnI CCWGG 2 cut(s) 100, 271
Alw21I GWGCWC 1 cut(s) 52
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 1 cut(s) 694
Aor51HI AGCGCT 1 cut(s) 593
AoxI GGCC 2 cut(s) 547, 644
ApeKI GCWGC 2 cut(s) 371, 455
ApoI RAATTY 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 2 cut(s) 594, 604
AspS9I GGNCC 1 cut(s) 269
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 627
BauI CACGAG 3 cut(s) 45, 51, 459
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 52
BbvI GCAGC 2 cut(s) 383, 442
BciT130I CCWGG 2 cut(s) 102, 273
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 447
BfmI CTRYAG 4 cut(s) 69, 91, 231, 437
BfoI RGCGCY 1 cut(s) 595
BfrI CTTAAG 1 cut(s) 577
BglII AGATCT 1 cut(s) 280
BisI GCNGC 2 cut(s) 372, 456
BlsI GCNGC 2 cut(s) 373, 457
Bme1390I CCNGG 3 cut(s) 102, 273, 451
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 102, 273, 451
BmsI GCATC 2 cut(s) 54, 655
BoxI GACNNNNGTC 1 cut(s) 579
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 450, 675
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 349, 658
Bse1I ACTGG 1 cut(s) 554
Bse3DI GCAATG 2 cut(s) 125, 420
Bse8I GATNNNNATC 1 cut(s) 164
BseBI CCWGG 2 cut(s) 102, 273
BseDI CCNNGG 2 cut(s) 450, 675
BseGI GGATG 1 cut(s) 591
BseJI GATNNNNATC 1 cut(s) 164
BseLI CCNNNNNNNGG 3 cut(s) 107, 349, 658
BseMI GCAATG 2 cut(s) 125, 420
BseMII CTCAG 1 cut(s) 517
BseNI ACTGG 1 cut(s) 554
BseXI GCAGC 2 cut(s) 383, 442
BshFI GGCC 2 cut(s) 549, 646
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 52
BsiSI CCGG 1 cut(s) 450
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 3 cut(s) 107, 349, 658
BsmAI GTCTC 1 cut(s) 447
BsmFI GGGAC 1 cut(s) 88
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 2 cut(s) 549, 646
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 52, 627
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 4 cut(s) 159, 280, 631, 686
BspACI CCGC 2 cut(s) 387, 684
BspANI GGCC 2 cut(s) 549, 646
BspCNI CTCAG 1 cut(s) 516
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 1 cut(s) 694
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 577
BsrDI GCAATG 2 cut(s) 125, 420
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 450, 675
BssMI GATC 4 cut(s) 159, 280, 631, 686
BssSI CACGAG 3 cut(s) 45, 51, 459
BssT1I CCWWGG 1 cut(s) 675
Bst2BI CACGAG 3 cut(s) 45, 51, 459
Bst2UI CCWGG 2 cut(s) 102, 273
Bst4CI ACNGT 1 cut(s) 441
Bst6I CTCTTC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 577
BstAUI TGTACA 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 551
BstDEI CTNAG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 591
BstH2I RGCGCY 1 cut(s) 595
BstHHI GCGC 2 cut(s) 594, 604
BstKTI GATC 4 cut(s) 162, 283, 634, 689
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 4 cut(s) 159, 280, 631, 686
BstMWI GCNNNNNNNGC 2 cut(s) 455, 608
BstNI CCWGG 2 cut(s) 102, 273
BstPAI GACNNNNGTC 1 cut(s) 579
BstSCI CCNGG 3 cut(s) 100, 271, 449
BstSFI CTRYAG 4 cut(s) 69, 91, 231, 437
BstV1I GCAGC 2 cut(s) 383, 442
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 2 cut(s) 549, 646
BtsCI GGATG 1 cut(s) 591
Cac8I GCNNGC 1 cut(s) 551
CfoI GCGC 2 cut(s) 594, 604
Cfr13I GGNCC 1 cut(s) 269
Csp6I GTAC 3 cut(s) 220, 259, 339
CviAII CATG 1 cut(s) 295
CviQI GTAC 3 cut(s) 220, 259, 339
DdeI CTNAG 1 cut(s) 503
DpnI GATC 4 cut(s) 161, 282, 633, 688
DpnII GATC 4 cut(s) 159, 280, 631, 686
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 675
Eco24I GRGCYC 1 cut(s) 627
Eco47I GGWCC 1 cut(s) 269
Eco47III AGCGCT 1 cut(s) 593
EcoRII CCWGG 2 cut(s) 100, 271
EcoT14I CCWWGG 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 1 cut(s) 298
FaqI GGGAC 1 cut(s) 88
FatI CATG 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 372, 456
FokI GGATG 1 cut(s) 598
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 2 cut(s) 372, 456
FspI TGCGCA 1 cut(s) 603
GlaI GCGC 2 cut(s) 593, 603
GluI GCNGC 2 cut(s) 372, 456
GsuI CTGGAG 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 595
HaeIII GGCC 2 cut(s) 549, 646
HapII CCGG 1 cut(s) 450
HhaI GCGC 2 cut(s) 594, 604
Hin1II CATG 1 cut(s) 298
Hin6I GCGC 2 cut(s) 592, 602
HinP1I GCGC 2 cut(s) 592, 602
HinfI GANTC 1 cut(s) 236
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 116
Hpy188I TCNGA 2 cut(s) 506, 585
Hpy188III TCNNGA 3 cut(s) 45, 426, 614
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 1 cut(s) 441
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 3 cut(s) 316, 413, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 608
HpyF3I CTNAG 1 cut(s) 503
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 1 cut(s) 298
HspAI GCGC 2 cut(s) 592, 602
KpnI GGTACC 1 cut(s) 342
Kzo9I GATC 4 cut(s) 159, 280, 631, 686
Lsp1109I GCAGC 2 cut(s) 383, 442
LweI GCATC 2 cut(s) 54, 655
MaeII ACGT 1 cut(s) 154
MalI GATC 4 cut(s) 161, 282, 633, 688
MboI GATC 4 cut(s) 159, 280, 631, 686
MboII GAAGA 5 cut(s) 33, 154, 197, 231, 371
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 2 cut(s) 52, 627
MluCI AATT 5 cut(s) 17, 201, 364, 429, 507
MmeI TCCRAC 2 cut(s) 37, 563
MnlI CCTC 3 cut(s) 100, 601, 681
MseI TTAA 4 cut(s) 525, 567, 578, 597
MspA1I CMGCKG 2 cut(s) 389, 684
MspCI CTTAAG 1 cut(s) 577
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 3 cut(s) 102, 273, 451
Mva1269I GAATGC 1 cut(s) 142
MvaI CCWGG 2 cut(s) 102, 273
MwoI GCNNNNNNNGC 2 cut(s) 455, 608
NciI CCSGG 1 cut(s) 451
NdeII GATC 4 cut(s) 159, 280, 631, 686
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 1 cut(s) 340
NsbI TGCGCA 1 cut(s) 603
NspV TTCGAA 1 cut(s) 242
PctI GAATGC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 236
PflMI CCANNNNNTGG 1 cut(s) 349
PkrI GCNGC 2 cut(s) 373, 457
PshAI GACNNNNGTC 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 100, 271
PspGI CCWGG 2 cut(s) 100, 271
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 269
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 3 cut(s) 221, 260, 340
RsaNI GTAC 3 cut(s) 220, 259, 339
SaqAI TTAA 4 cut(s) 525, 567, 578, 597
SatI GCNGC 2 cut(s) 372, 456
Sau3AI GATC 4 cut(s) 159, 280, 631, 686
Sau96I GGNCC 1 cut(s) 269
ScrFI CCNGG 3 cut(s) 102, 273, 451
SduI GDGCHC 2 cut(s) 52, 627
SetI ASST 6 cut(s) 41, 157, 225, 522, 673, 694
SfaNI GCATC 2 cut(s) 54, 655
SfcI CTRYAG 4 cut(s) 69, 91, 231, 437
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
Sse9I AATT 5 cut(s) 17, 201, 364, 429, 507
SsiI CCGC 2 cut(s) 387, 684
StyD4I CCNGG 3 cut(s) 100, 271, 449
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 1 cut(s) 441
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 242
TasI AATT 5 cut(s) 17, 201, 364, 429, 507
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 4 cut(s) 525, 567, 578, 597
Tru9I TTAA 4 cut(s) 525, 567, 578, 597
TseI GCWGC 2 cut(s) 371, 455
TspDTI ATGAA 6 cut(s) 154, 198, 501, 510, 554, 602
Van91I CCANNNNNTGG 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.