AT1G13050

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009904
Physical Location & Seq
Forward (+)
4449713 .. 4451742
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G13050.1

Sequence Viewer

Length: 954 bp
ATGTCTCACCACCACTATGAAACCAACCCTCATTTCGTTCAATTTTCCTTGCAGGATCAACATCAAGGTGGTCCTTCTAGCTCATGGAACTCTCCGCACCATCACCAGATCCCACAGGCTCACTCAGTTGCTCCACCCAGGGTGAAAATAAAGACTCGGGGACGCCACCAGACTGAGCCACCTGAAACAATCCATGAATCGCCTTCCTCAAGACCCTTGCCACTGAGACCGGAGGAACCATTACCACCACGCCATAATCCAAACTCTGCCAGGCCATTGCAATTGAGCCCTGAGGAGCAACGACCTCCACACCGTGGCTATGGATCTGAACCAACTCCATGGAGAAGAGCTCCAACACGACCAGCTTATCAGCAAGGCCCAAAGAGGACCAAACCCATGACATTGCCAGCCACAATCTGCTGTGCAATTCTCCTGATCGTCCTAATTTTATCAGGTCTCATTCTCCTCTTAGTCTACCTCGCCAACCGTCCACGCTCACCATACTTCGACATCTCAGCAGCAACCCTAAACACCGCCAATCTGGACATGGGATATGTTCTAAATGGAGATCTCGCGGTTGTGGTAAACTTCACAAATCCAAGCAAGAAAAGCAGTGTGGACTTCAGCTACGTGATGTTCGAGCTCTACTTCTACAACACACTCATAGCGACTGAACACATCGAGCCATTCATTGTACCAAAGGGAATGTCAATGTTCACAAGCTTCCATCTCGTCAGCAGTCAGGTACAAATTCAGATGATTCAGAGTCAGGACTTGCAGCTGCAGCTCGGAACTGGTCCAGTGTTGTTGAATTTGAGAGGAACGTTTCACGCTCGCTCGAACCTTGGGTCGTTGATGAGATACTCTTATTGGTTGCACACACAGTGCAGCATCTCTTTAAATACCCCTCCTGCAGGGACCATGCGAGCAAGAAGATGCAATACAAAACGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

317

Amino Acids

35.81

Weight (kDa)

9.81

Isoelectric Point (pI)

63.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 474
AccII CGCG 1 cut(s) 575
AciI CCGC 3 cut(s) 95, 534, 575
AclI AACGTT 1 cut(s) 824
AclWI GGATC 3 cut(s) 63, 103, 331
AcsI RAATTY 2 cut(s) 750, 811
AcuI CTGAAG 1 cut(s) 607
AcyI GRCGYC 1 cut(s) 163
AdeI CACNNNGTG 2 cut(s) 314, 885
AfaI GTAC 2 cut(s) 696, 747
AfiI CCNNNNNNNGG 2 cut(s) 314, 914
AgsI TTSAA 2 cut(s) 41, 811
AjnI CCWGG 2 cut(s) 137, 269
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 8 cut(s) 81, 350, 365, 627, 643, 723, 781, 787
AluI AGCT 8 cut(s) 81, 350, 365, 627, 643, 723, 781, 787
Alw21I GWGCWC 2 cut(s) 352, 645
Alw26I GTCTC 3 cut(s) 9, 220, 461
AlwI GGATC 3 cut(s) 63, 103, 331
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 2 cut(s) 272, 376
ApeKI GCWGC 5 cut(s) 518, 778, 781, 784, 888
ApoI RAATTY 2 cut(s) 750, 811
ArsI GACNNNNNNTTYG 4 cut(s) 692, 724, 833, 865
AspS9I GGNCC 5 cut(s) 71, 377, 387, 797, 918
AsuHPI GGTGA 3 cut(s) 95, 154, 489
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 4 cut(s) 71, 387, 797, 918
AxyI CCTNAGG 1 cut(s) 291
BanII GRGCYC 3 cut(s) 290, 352, 645
BarI GAAGNNNNNNTAC 1 cut(s) 925
Bbv12I GWGCWC 2 cut(s) 352, 645
BbvI GCAGC 5 cut(s) 530, 768, 790, 796, 900
BccI CCATC 2 cut(s) 108, 735
BciT130I CCWGG 2 cut(s) 139, 271
BcoDI GTCTC 3 cut(s) 9, 220, 461
BfaI CTAG 2 cut(s) 78, 952
BfmI CTRYAG 2 cut(s) 782, 912
BglII AGATCT 1 cut(s) 568
BisI GCNGC 5 cut(s) 519, 779, 782, 785, 889
BlsI GCNGC 5 cut(s) 520, 780, 783, 786, 890
Bme1390I CCNGG 2 cut(s) 139, 271
Bme18I GGWCC 4 cut(s) 71, 387, 797, 918
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 5 cut(s) 71, 377, 387, 797, 918
BmiI GGNNCC 2 cut(s) 237, 919
BmrFI CCNGG 2 cut(s) 139, 271
BmsI GCATC 2 cut(s) 900, 926
BplI GAGNNNNNCTC 2 cut(s) 334, 366
BpuEI CTTGAG 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 631
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 163
BsaI GGTCTC 2 cut(s) 220, 461
BsaJI CCNNGG 5 cut(s) 137, 138, 313, 338, 844
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 914
Bse1I ACTGG 2 cut(s) 799, 800
Bse21I CCTNAGG 1 cut(s) 291
Bse3DI GCAATG 2 cut(s) 275, 401
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 2 cut(s) 139, 271
BseDI CCNNGG 5 cut(s) 137, 138, 313, 338, 844
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 314, 914
BseMI GCAATG 2 cut(s) 275, 401
BseMII CTCAG 5 cut(s) 138, 165, 215, 282, 528
BseNI ACTGG 2 cut(s) 799, 800
BseRI GAGGAG 2 cut(s) 308, 455
BseXI GCAGC 5 cut(s) 530, 768, 790, 796, 900
BsgI GTGCAG 1 cut(s) 907
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 2 cut(s) 274, 378
BsiHKAI GWGCWC 2 cut(s) 352, 645
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 1 cut(s) 230
BslFI GGGAC 2 cut(s) 174, 931
BslI CCNNNNNNNGG 2 cut(s) 314, 914
BsmAI GTCTC 3 cut(s) 9, 220, 461
BsmFI GGGAC 2 cut(s) 174, 931
BsnI GGCC 2 cut(s) 274, 378
Bso31I GGTCTC 2 cut(s) 220, 461
BsoBI CYCGRG 1 cut(s) 156
Bsp1286I GDGCHC 3 cut(s) 290, 352, 645
Bsp143I GATC 5 cut(s) 55, 108, 323, 435, 568
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 3 cut(s) 95, 534, 575
BspANI GGCC 2 cut(s) 274, 378
BspCNI CTCAG 5 cut(s) 137, 166, 216, 283, 527
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 2 cut(s) 237, 919
BspMAI CTGCAG 2 cut(s) 786, 916
BspPI GGATC 3 cut(s) 63, 103, 331
BspQI GCTCTTC 1 cut(s) 340
BspTNI GGTCTC 2 cut(s) 220, 461
BsrDI GCAATG 2 cut(s) 275, 401
BsrI ACTGG 2 cut(s) 799, 800
BssECI CCNNGG 5 cut(s) 137, 138, 313, 338, 844
BssMI GATC 5 cut(s) 55, 108, 323, 435, 568
BssNI GRCGYC 1 cut(s) 163
BssT1I CCWWGG 2 cut(s) 338, 844
Bst2UI CCWGG 2 cut(s) 139, 271
Bst4CI ACNGT 3 cut(s) 314, 488, 885
Bst6I CTCTTC 1 cut(s) 340
BstACI GRCGYC 1 cut(s) 163
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 3 cut(s) 408, 835, 927
BstDEI CTNAG 6 cut(s) 124, 174, 224, 291, 469, 514
BstDSI CCRYGG 2 cut(s) 313, 338
BstENI CCTNNNNNAGG 1 cut(s) 912
BstFNI CGCG 1 cut(s) 575
BstKTI GATC 5 cut(s) 58, 111, 326, 438, 571
BstMAI GTCTC 3 cut(s) 9, 220, 461
BstMBI GATC 5 cut(s) 55, 108, 323, 435, 568
BstMWI GCNNNNNNNGC 2 cut(s) 609, 784
BstNI CCWGG 2 cut(s) 139, 271
BstSCI CCNGG 2 cut(s) 137, 269
BstSFI CTRYAG 2 cut(s) 782, 912
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 5 cut(s) 530, 768, 790, 796, 900
BstX2I RGATCY 3 cut(s) 108, 323, 568
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 3 cut(s) 108, 323, 568
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 2 cut(s) 274, 378
BtgI CCRYGG 2 cut(s) 313, 338
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 4 cut(s) 221, 619, 807, 890
Cac8I GCNNGC 3 cut(s) 408, 835, 927
Cfr13I GGNCC 5 cut(s) 71, 377, 387, 797, 918
CseI GACGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 695, 746
CviAII CATG 6 cut(s) 84, 194, 339, 397, 547, 922
CviQI GTAC 2 cut(s) 695, 746
DdeI CTNAG 6 cut(s) 124, 174, 224, 291, 469, 514
DpnI GATC 5 cut(s) 57, 110, 325, 437, 570
DpnII GATC 5 cut(s) 55, 108, 323, 435, 568
DraI TTTAAA 1 cut(s) 900
DraIII CACNNNGTG 2 cut(s) 314, 885
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Ecl136II GAGCTC 2 cut(s) 350, 643
Eco130I CCWWGG 2 cut(s) 338, 844
Eco24I GRGCYC 3 cut(s) 290, 352, 645
Eco31I GGTCTC 2 cut(s) 220, 461
Eco47I GGWCC 4 cut(s) 71, 387, 797, 918
Eco53kI GAGCTC 2 cut(s) 350, 643
Eco57I CTGAAG 1 cut(s) 607
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 156
EcoICRI GAGCTC 2 cut(s) 350, 643
EcoNI CCTNNNNNAGG 1 cut(s) 912
EcoRII CCWGG 2 cut(s) 137, 269
EcoT14I CCWWGG 2 cut(s) 338, 844
EcoT38I GRGCYC 3 cut(s) 290, 352, 645
ErhI CCWWGG 2 cut(s) 338, 844
FaeI CATG 6 cut(s) 87, 197, 342, 400, 550, 925
FaqI GGGAC 2 cut(s) 174, 931
FatI CATG 6 cut(s) 83, 193, 338, 396, 546, 921
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 5 cut(s) 519, 779, 782, 785, 889
FriOI GRGCYC 3 cut(s) 290, 352, 645
Fsp4HI GCNGC 5 cut(s) 519, 779, 782, 785, 889
FspBI CTAG 2 cut(s) 78, 952
GluI GCNGC 5 cut(s) 519, 779, 782, 785, 889
HaeIII GGCC 2 cut(s) 274, 378
HapII CCGG 1 cut(s) 230
HgaI GACGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 6 cut(s) 87, 197, 342, 400, 550, 925
HindIII AAGCTT 1 cut(s) 721
HinfI GANTC 4 cut(s) 154, 197, 760, 766
HpaII CCGG 1 cut(s) 230
HphI GGTGA 3 cut(s) 95, 154, 489
Hpy166II GTNNAC 5 cut(s) 475, 491, 586, 619, 717
Hpy188I TCNGA 4 cut(s) 328, 756, 765, 791
Hpy188III TCNNGA 4 cut(s) 210, 433, 542, 770
Hpy8I GTNNAC 5 cut(s) 475, 491, 586, 619, 717
HpyAV CCTTC 2 cut(s) 84, 213
HpyCH4III ACNGT 3 cut(s) 314, 488, 885
HpyCH4IV ACGT 2 cut(s) 630, 824
HpyCH4V TGCA 9 cut(s) 52, 280, 425, 778, 784, 877, 888, 914, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 609, 784
HpyF3I CTNAG 6 cut(s) 124, 174, 224, 291, 469, 514
HpySE526I ACGT 2 cut(s) 630, 824
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 6 cut(s) 87, 197, 342, 400, 550, 925
Kzo9I GATC 5 cut(s) 55, 108, 323, 435, 568
LguI GCTCTTC 1 cut(s) 340
LmnI GCTCC 3 cut(s) 136, 295, 355
Lsp1109I GCAGC 5 cut(s) 530, 768, 790, 796, 900
LweI GCATC 2 cut(s) 900, 926
MaeI CTAG 2 cut(s) 78, 952
MaeII ACGT 2 cut(s) 630, 824
MalI GATC 5 cut(s) 57, 110, 325, 437, 570
MboI GATC 5 cut(s) 55, 108, 323, 435, 568
MboII GAAGA 2 cut(s) 357, 945
MfeI CAATTG 1 cut(s) 281
MflI RGATCY 3 cut(s) 108, 323, 568
MhlI GDGCHC 3 cut(s) 290, 352, 645
MluCI AATT 6 cut(s) 41, 281, 426, 444, 750, 811
MlyI GAGTC 2 cut(s) 148, 775
MmeI TCCRAC 1 cut(s) 377
MseI TTAA 1 cut(s) 899
MslI CAYNNNNRTG 2 cut(s) 15, 66
MspA1I CMGCKG 1 cut(s) 781
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 139, 271
MunI CAATTG 1 cut(s) 281
MvaI CCWGG 2 cut(s) 139, 271
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 2 cut(s) 609, 784
NcoI CCATGG 1 cut(s) 338
NdeII GATC 5 cut(s) 55, 108, 323, 435, 568
NlaIII CATG 6 cut(s) 87, 197, 342, 400, 550, 925
NlaIV GGNNCC 2 cut(s) 237, 919
PasI CCCWGGG 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfeI GAWTC 2 cut(s) 197, 760
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 5 cut(s) 520, 780, 783, 786, 890
PleI GAGTC 2 cut(s) 148, 774
PpsI GAGTC 2 cut(s) 148, 774
Ppu21I YACGTR 1 cut(s) 631
Psp124BI GAGCTC 2 cut(s) 352, 645
Psp1406I AACGTT 1 cut(s) 824
Psp6I CCWGG 2 cut(s) 137, 269
PspGI CCWGG 2 cut(s) 137, 269
PspN4I GGNNCC 2 cut(s) 237, 919
PspPI GGNCC 5 cut(s) 71, 377, 387, 797, 918
PstI CTGCAG 2 cut(s) 786, 916
PsuI RGATCY 3 cut(s) 108, 323, 568
PvuII CAGCTG 1 cut(s) 781
RsaI GTAC 2 cut(s) 696, 747
RsaNI GTAC 2 cut(s) 695, 746
RseI CAYNNNNRTG 2 cut(s) 15, 66
SacI GAGCTC 2 cut(s) 352, 645
SapI GCTCTTC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 899
SatI GCNGC 5 cut(s) 519, 779, 782, 785, 889
Sau3AI GATC 5 cut(s) 55, 108, 323, 435, 568
Sau96I GGNCC 5 cut(s) 71, 377, 387, 797, 918
SbfI CCTGCAGG 1 cut(s) 916
SchI GAGTC 2 cut(s) 148, 775
ScrFI CCNGG 2 cut(s) 139, 271
SdaI CCTGCAGG 1 cut(s) 916
SduI GDGCHC 3 cut(s) 290, 352, 645
SfaNI GCATC 2 cut(s) 900, 926
SfcI CTRYAG 2 cut(s) 782, 912
SinI GGWCC 4 cut(s) 71, 387, 797, 918
SmiMI CAYNNNNRTG 2 cut(s) 15, 66
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
Sse8387I CCTGCAGG 1 cut(s) 916
Sse9I AATT 6 cut(s) 41, 281, 426, 444, 750, 811
SsiI CCGC 3 cut(s) 95, 534, 575
SspMI CTAG 2 cut(s) 78, 952
SstI GAGCTC 2 cut(s) 352, 645
StyD4I CCNGG 2 cut(s) 137, 269
StyI CCWWGG 2 cut(s) 338, 844
TaaI ACNGT 3 cut(s) 314, 488, 885
TaiI ACGT 2 cut(s) 633, 827
TaqI TCGA 4 cut(s) 507, 639, 681, 839
TasI AATT 6 cut(s) 41, 281, 426, 444, 750, 811
TfiI GAWTC 2 cut(s) 197, 760
Tru1I TTAA 1 cut(s) 899
Tru9I TTAA 1 cut(s) 899
TscAI CASTG 4 cut(s) 228, 619, 807, 890
TseI GCWGC 5 cut(s) 518, 778, 781, 784, 888
TspDTI ATGAA 3 cut(s) 33, 210, 679
TspRI CASTG 4 cut(s) 228, 619, 807, 890
Van91I CCANNNNNTGG 1 cut(s) 314
VpaK11BI GGWCC 4 cut(s) 71, 387, 797, 918
XagI CCTNNNNNAGG 1 cut(s) 912
XapI RAATTY 2 cut(s) 750, 811
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 2 cut(s) 78, 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.