pycom13g09020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009904
Physical Location & Seq
Forward (+)
6021859 .. 6022545
687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09020.1

Sequence Viewer

Length: 687 bp
ATGGTGCCAGCCTCGCATCATGGTCGCGAAGACGAGCAACAACGTTGGCCATACCCACAAGGGCAGCCGCATAGCCAGCATACTCGCCCACACGGCTTGCACATACCACGGCCTCAAAAAACCAATGTCTTCGCATGGTCAGGAGCCATTTGCTGTGCAATTTTTTGGGTTCTCATTATCTTAACCGGCCTAGTGGTTCTCATAGTCTATCTTGTCTTTCGTCCCCGGACTCCGAAATTCGACGTCTCTTCCGCCACACTCAACGCAGCGTATCTTGACATGGGATATCTTCTCAATGCCGACGTTACAGTGCTGGCAAACTTCTCCAACCCAAACAAGAAGGTCAGCGTTGACTTTAGCAACCTGATCATTGATCTTTATTATGGAAATACCCGTGTTGCTACCCAGTACATAGAACCCTTCTCTGCATCGAGGCGTCAGTCCATGTTCGCAAATGTTCATATGGTGGCCAGCCAGGTTCGTCTCGGAGTGCTGGAAAGCATAAGGCTCAAGAAGGAGATGGAGACCGATCGGGCCAGATTTGAGGTCAAGGGGTATTTCCGAGCACGAGCTAACTTCGGAAAGATCCTCCGGTACTCGTACTGGTTGCACGGCGACTGCCAAGTTGTGCTCACTAGACCTCCTGATGGAGTTCTGGTGACCAGAAAATGCAAGACAAAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

229

Amino Acids

26.1

Weight (kDa)

9.82

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 27
AciI CCGC 2 cut(s) 68, 252
AclI AACGTT 1 cut(s) 43
AclWI GGATC 1 cut(s) 580
AcoI YGGCCR 2 cut(s) 47, 468
AcsI RAATTY 1 cut(s) 236
AcyI GRCGYC 2 cut(s) 243, 436
AfaI GTAC 3 cut(s) 410, 596, 602
AfiI CCNNNNNNNGG 1 cut(s) 647
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 1 cut(s) 572
AluI AGCT 1 cut(s) 572
Alw21I GWGCWC 2 cut(s) 568, 633
Alw26I GTCTC 3 cut(s) 250, 488, 518
AlwI GGATC 1 cut(s) 580
AoxI GGCC 5 cut(s) 47, 110, 187, 468, 534
ApeKI GCWGC 2 cut(s) 64, 266
ApoI RAATTY 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 534
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 670
BalI TGGCCA 2 cut(s) 49, 470
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 567
BbsI GAAGAC 2 cut(s) 36, 121
Bbv12I GWGCWC 2 cut(s) 568, 633
BbvI GCAGC 2 cut(s) 76, 278
BccI CCATC 2 cut(s) 514, 641
BceAI ACGGC 3 cut(s) 109, 125, 628
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 366
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 3 cut(s) 250, 488, 518
BfaI CTAG 2 cut(s) 191, 636
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 3 cut(s) 65, 68, 267
BlsI GCNGC 3 cut(s) 66, 69, 268
Bme1390I CCNGG 2 cut(s) 226, 476
BmgT120I GGNCC 1 cut(s) 534
BmiI GGNNCC 2 cut(s) 6, 145
BmrFI CCNGG 2 cut(s) 226, 476
BmrI ACTGGG 1 cut(s) 400
BmsI GCATC 2 cut(s) 25, 437
BmuI ACTGGG 1 cut(s) 400
BpiI GAAGAC 2 cut(s) 36, 121
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 226
BsaHI GRCGYC 2 cut(s) 243, 436
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 107, 224
BsaWI WCCGGW 1 cut(s) 591
BsaXI ACNNNNNCTCC 4 cut(s) 625, 642, 655, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 406, 608
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 2 cut(s) 107, 224
BseLI CCNNNNNNNGG 1 cut(s) 647
BseNI ACTGG 2 cut(s) 406, 608
BseXI GCAGC 2 cut(s) 76, 278
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 532
BshFI GGCC 5 cut(s) 49, 112, 189, 470, 536
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 532
BsiHKAI GWGCWC 2 cut(s) 568, 633
BsiSI CCGG 3 cut(s) 186, 226, 592
BslFI GGGAC 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 3 cut(s) 250, 488, 518
BsmBI CGTCTC 2 cut(s) 250, 488
BsmFI GGGAC 1 cut(s) 207
BsnI GGCC 5 cut(s) 49, 112, 189, 470, 536
Bso31I GGTCTC 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 568, 633
Bsp143I GATC 4 cut(s) 366, 373, 529, 585
Bsp68I TCGCGA 1 cut(s) 27
BspACI CCGC 2 cut(s) 68, 252
BspANI GGCC 5 cut(s) 49, 112, 189, 470, 536
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 6, 145
BspPI GGATC 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 518
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 406, 608
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 107, 224
BssMI GATC 4 cut(s) 366, 373, 529, 585
BssNI GRCGYC 2 cut(s) 243, 436
BssSI CACGAG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 2 cut(s) 243, 436
BstC8I GCNNGC 5 cut(s) 9, 77, 98, 315, 472
BstDSI CCRYGG 1 cut(s) 107
BstEII GGTNACC 1 cut(s) 658
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 4 cut(s) 369, 376, 532, 588
BstMAI GTCTC 3 cut(s) 250, 488, 518
BstMBI GATC 4 cut(s) 366, 373, 529, 585
BstMCI CGRYCG 1 cut(s) 532
BstMWI GCNNNNNNNGC 3 cut(s) 13, 76, 93
BstNI CCWGG 1 cut(s) 476
BstPI GGTNACC 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 224, 474
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 2 cut(s) 76, 278
BstV2I GAAGAC 2 cut(s) 36, 121
BstX2I RGATCY 1 cut(s) 585
BstYI RGATCY 1 cut(s) 585
BsuRI GGCC 5 cut(s) 49, 112, 189, 470, 536
BtgI CCRYGG 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 315
BtuMI TCGCGA 1 cut(s) 27
Cac8I GCNNGC 5 cut(s) 9, 77, 98, 315, 472
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 534
CseI GACGC 1 cut(s) 425
Csp6I GTAC 3 cut(s) 409, 595, 601
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviAII CATG 4 cut(s) 20, 135, 280, 445
CviQI GTAC 3 cut(s) 409, 595, 601
DpnI GATC 4 cut(s) 368, 375, 531, 587
DpnII GATC 4 cut(s) 366, 373, 529, 585
EaeI YGGCCR 2 cut(s) 47, 468
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 518
Eco32I GATATC 1 cut(s) 287
Eco91I GGTNACC 1 cut(s) 658
EcoO65I GGTNACC 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 474
EcoRV GATATC 1 cut(s) 287
Esp3I CGTCTC 2 cut(s) 250, 488
FaeI CATG 4 cut(s) 23, 138, 283, 448
FaqI GGGAC 1 cut(s) 207
FatI CATG 4 cut(s) 19, 134, 279, 444
FauNDI CATATG 1 cut(s) 462
FbaI TGATCA 1 cut(s) 366
Fnu4HI GCNGC 3 cut(s) 65, 68, 267
Fsp4HI GCNGC 3 cut(s) 65, 68, 267
FspBI CTAG 2 cut(s) 191, 636
GluI GCNGC 3 cut(s) 65, 68, 267
HaeIII GGCC 5 cut(s) 49, 112, 189, 470, 536
HapII CCGG 3 cut(s) 186, 226, 592
HgaI GACGC 1 cut(s) 425
Hin1I GRCGYC 2 cut(s) 243, 436
Hin1II CATG 4 cut(s) 23, 138, 283, 448
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 229
HpaII CCGG 3 cut(s) 186, 226, 592
HphI GGTGA 1 cut(s) 670
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 4 cut(s) 234, 488, 563, 581
Hpy188III TCNNGA 5 cut(s) 26, 141, 275, 511, 644
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 2 cut(s) 245, 305
HpyAV CCTTC 3 cut(s) 334, 430, 508
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 3 cut(s) 43, 243, 303
HpyCH4V TGCA 5 cut(s) 100, 158, 428, 610, 672
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 76, 93
HpySE526I ACGT 3 cut(s) 43, 243, 303
Hsp92I GRCGYC 2 cut(s) 243, 436
Hsp92II CATG 4 cut(s) 23, 138, 283, 448
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 4 cut(s) 366, 373, 529, 585
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 2 cut(s) 76, 278
LweI GCATC 2 cut(s) 25, 437
MaeI CTAG 2 cut(s) 191, 636
MaeII ACGT 3 cut(s) 43, 243, 303
MaeIII GTNAC 2 cut(s) 304, 658
MalI GATC 4 cut(s) 368, 375, 531, 587
MboI GATC 4 cut(s) 366, 373, 529, 585
MboII GAAGA 4 cut(s) 41, 121, 240, 281
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 2 cut(s) 568, 633
MlsI TGGCCA 2 cut(s) 49, 470
MluCI AATT 2 cut(s) 159, 236
MluNI TGGCCA 2 cut(s) 49, 470
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 6 cut(s) 22, 123, 426, 538, 599, 651
Mox20I TGGCCA 2 cut(s) 49, 470
MscI TGGCCA 2 cut(s) 49, 470
MseI TTAA 1 cut(s) 182
Msp20I TGGCCA 2 cut(s) 49, 470
MspI CCGG 3 cut(s) 186, 226, 592
MspR9I CCNGG 2 cut(s) 226, 476
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 13, 76, 93
NciI CCSGG 1 cut(s) 226
NdeI CATATG 1 cut(s) 462
NdeII GATC 4 cut(s) 366, 373, 529, 585
NlaIII CATG 4 cut(s) 23, 138, 283, 448
NlaIV GGNNCC 2 cut(s) 6, 145
NmuCI GTSAC 1 cut(s) 658
NruI TCGCGA 1 cut(s) 27
PkrI GCNGC 3 cut(s) 66, 69, 268
Ple19I CGATCG 1 cut(s) 532
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp1406I AACGTT 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 658
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 2 cut(s) 6, 145
PspPI GGNCC 1 cut(s) 534
PsuI RGATCY 1 cut(s) 585
PvuI CGATCG 1 cut(s) 532
RruI TCGCGA 1 cut(s) 27
RsaI GTAC 3 cut(s) 410, 596, 602
RsaNI GTAC 3 cut(s) 409, 595, 601
SaqAI TTAA 1 cut(s) 182
SatI GCNGC 3 cut(s) 65, 68, 267
Sau3AI GATC 4 cut(s) 366, 373, 529, 585
Sau96I GGNCC 1 cut(s) 534
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 226, 476
SduI GDGCHC 2 cut(s) 568, 633
SetI ASST 9 cut(s) 46, 246, 306, 345, 366, 480, 549, 574, 643
SfaNI GCATC 2 cut(s) 25, 437
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 2 cut(s) 159, 236
SsiI CCGC 2 cut(s) 68, 252
SspMI CTAG 2 cut(s) 191, 636
StyD4I CCNGG 2 cut(s) 224, 474
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 3 cut(s) 46, 246, 306
TaqI TCGA 2 cut(s) 240, 431
TaqII GACCGA 1 cut(s) 542
TasI AATT 2 cut(s) 159, 236
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TscAI CASTG 1 cut(s) 315
TseFI GTSAC 1 cut(s) 658
TseI GCWGC 2 cut(s) 64, 266
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 1 cut(s) 449
TspRI CASTG 1 cut(s) 315
XapI RAATTY 1 cut(s) 236
XspI CTAG 2 cut(s) 191, 636
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.