MD13G1103200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009904
Physical Location & Seq
Forward (+)
7367515 .. 7368651
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1103200.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGTCTCACCGTGGTGACACCAACCCCCATTTTGTCCGGCCATATCCGTCACAATATCCAGAACAACTTCCCCAAACATCCCCACACAGTTCATCGGTGCCACTCCCACATCACCCTGGGCAGCACCCTGAGCAGCCCAACCTCAGACCACAATGGCCTGGACCGCATCCCCCCGAACAACAAGTTGAGCCAGGAACAAGACAATTACAGCCACCCCGCCGGCCCCGGTCTAAGCATCCGGCGGGTCATTTGGTGTCATTTCATCCAAACCTTGAAGAACATCCTCCTCGGACAGACCAGCAAACCCCCCACCTTGATCATGAAGACCAACAGCCTCGGACAAAACATCCAGGCCAGAAAACCCATGTGGCTGGCCCATACGATGATCATGAAGGAGAACAGCGTCCTCTGAAAAAGCATCAAGGCCAGAAAACCCCCCCTATAGTTCCAATGGTGCCAGCCCCGCATCATGATCGCGAAGACGAGCAACATCGTCGGCCATACCCACAAGGGCAGCCGCATGGCCGGCATACTCGCCAACAGGGCTTGCACGCACCACGGCCTAAAAAAACCAATGTCCTCGCATGGTCAGGAGCCATTTGCTGTGCAATTTTTTGGGTTCTCGTTATCTTAACCGGCCTAGTGGTTCTCATAGTCTATCTTGTTTTTCGTCCCCGGACTCCGAAATTCGACGTCTCTTCCGCCACACTAAACGCAGCGTATCTTGACATGGGATATCTTCTCAATGCCGACGTTACAGTGCTGGCAAACTTCACCAACCCAAACAAGAAGGTCAGCGTTGACTTTAGCAACCTGATCATTGATCTTTATTACGGAAATACCCGCGTTGCTACGCAGTACATAGAACCCTTCTCTGCACAGAGGCGTCAGTCCATGTTCGCAAATGTTCATATGGTGGCTAGCCAGGTTCGTCTCGGTGTGCTGGAAAGCATAAGGCTCAAGAAGGAGATGGAGACCGATCGGGCCAGATTTGAGGTCAAGGGGTATTTCCGAGCACGAGCTAACTTCGGAAACATCCTCCGGTACTCCTACTGGTTGCACGGGGACTGCCAAGTTGTGCTCACTAGACCTCCTGATGGAGTTCTGGTCGCCAGAAAATGCAAGACAAAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

379

Amino Acids

43.23

Weight (kDa)

9.73

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 696
AccB1I GGYRCC 2 cut(s) 97, 454
AccII CGCG 2 cut(s) 477, 846
AciI CCGC 7 cut(s) 164, 217, 242, 464, 518, 702, 844
AcoI YGGCCR 3 cut(s) 38, 497, 523
AcsI RAATTY 1 cut(s) 686
AcyI GRCGYC 2 cut(s) 693, 886
AfaI GTAC 2 cut(s) 860, 1046
AfiI CCNNNNNNNGG 1 cut(s) 1097
AgsI TTSAA 1 cut(s) 275
AjnI CCWGG 5 cut(s) 115, 157, 190, 349, 924
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 1 cut(s) 1022
AluI AGCT 1 cut(s) 1022
Alw21I GWGCWC 2 cut(s) 1018, 1083
Alw26I GTCTC 4 cut(s) 9, 700, 938, 968
ApeKI GCWGC 4 cut(s) 121, 133, 514, 716
ApoI RAATTY 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 4 cut(s) 161, 222, 374, 984
AsuC2I CCSGG 2 cut(s) 226, 676
AsuHPI GGTGA 3 cut(s) 26, 104, 766
AsuNHI GCTAGC 1 cut(s) 920
AvaII GGWCC 1 cut(s) 161
BanI GGYRCC 2 cut(s) 97, 454
BauI CACGAG 1 cut(s) 1017
BbsI GAAGAC 2 cut(s) 330, 486
Bbv12I GWGCWC 2 cut(s) 1018, 1083
BbvI GCAGC 4 cut(s) 133, 145, 526, 728
BccI CCATC 2 cut(s) 964, 1091
BceAI ACGGC 1 cut(s) 575
BcgI CGANNNNNNTGC 4 cut(s) 455, 476, 489, 510
BciT130I CCWGG 5 cut(s) 117, 159, 192, 351, 926
BclI TGATCA 3 cut(s) 316, 385, 816
BcnI CCSGG 2 cut(s) 226, 676
BcoDI GTCTC 4 cut(s) 9, 700, 938, 968
BfaI CTAG 3 cut(s) 641, 921, 1086
BfmI CTRYAG 1 cut(s) 441
BglI GCCNNNNNGGC 1 cut(s) 543
BisI GCNGC 5 cut(s) 122, 134, 515, 518, 717
BlsI GCNGC 5 cut(s) 123, 135, 516, 519, 718
Bme1390I CCNGG 7 cut(s) 117, 159, 192, 226, 351, 676, 926
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 4 cut(s) 161, 222, 374, 984
BmiI GGNNCC 4 cut(s) 99, 224, 456, 595
BmrFI CCNGG 7 cut(s) 117, 159, 192, 226, 351, 676, 926
BmsI GCATC 4 cut(s) 175, 244, 427, 475
BmtI GCTAGC 1 cut(s) 924
BpiI GAAGAC 2 cut(s) 330, 486
Bpu10I CCTNAGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 944
BpuMI CCSGG 2 cut(s) 226, 676
BsaHI GRCGYC 2 cut(s) 693, 886
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 8 cut(s) 10, 115, 116, 224, 287, 335, 557, 674
BsaWI WCCGGW 1 cut(s) 1041
BsaXI ACNNNNNCTCC 4 cut(s) 1075, 1092, 1105, 1122
Bsc4I CCNNNNNNNGG 1 cut(s) 1097
Bse118I RCCGGY 3 cut(s) 219, 525, 635
Bse1I ACTGG 1 cut(s) 1058
BseBI CCWGG 5 cut(s) 117, 159, 192, 351, 926
BseDI CCNNGG 8 cut(s) 10, 115, 116, 224, 287, 335, 557, 674
BseGI GGATG 7 cut(s) 77, 166, 235, 262, 280, 346, 1035
BseLI CCNNNNNNNGG 1 cut(s) 1097
BseMII CTCAG 2 cut(s) 120, 157
BseNI ACTGG 1 cut(s) 1058
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 4 cut(s) 133, 145, 526, 728
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 2 cut(s) 477, 846
Bsh1285I CGRYCG 1 cut(s) 982
BshNI GGYRCC 2 cut(s) 97, 454
BsiEI CGRYCG 1 cut(s) 982
BsiHKAI GWGCWC 2 cut(s) 1018, 1083
BsiSI CCGG 8 cut(s) 37, 220, 226, 239, 526, 636, 676, 1042
BslFI GGGAC 2 cut(s) 657, 1079
BslI CCNNNNNNNGG 1 cut(s) 1097
BsmAI GTCTC 4 cut(s) 9, 700, 938, 968
BsmBI CGTCTC 2 cut(s) 700, 938
BsmFI GGGAC 2 cut(s) 657, 1079
Bso31I GGTCTC 1 cut(s) 968
Bsp1286I GDGCHC 2 cut(s) 1018, 1083
Bsp143I GATC 6 cut(s) 316, 385, 472, 816, 823, 979
Bsp68I TCGCGA 1 cut(s) 477
BspACI CCGC 7 cut(s) 164, 217, 242, 464, 518, 702, 844
BspCNI CTCAG 2 cut(s) 121, 156
BspFNI CGCG 2 cut(s) 477, 846
BspHI TCATGA 3 cut(s) 319, 388, 469
BspLI GGNNCC 4 cut(s) 99, 224, 456, 595
BspOI GCTAGC 1 cut(s) 924
BspT107I GGYRCC 2 cut(s) 97, 454
BspTNI GGTCTC 1 cut(s) 968
BsrFI RCCGGY 3 cut(s) 219, 525, 635
BsrI ACTGG 1 cut(s) 1058
BssAI RCCGGY 3 cut(s) 219, 525, 635
BssECI CCNNGG 8 cut(s) 10, 115, 116, 224, 287, 335, 557, 674
BssMI GATC 6 cut(s) 316, 385, 472, 816, 823, 979
BssNI GRCGYC 2 cut(s) 693, 886
BssSI CACGAG 1 cut(s) 1017
Bst2BI CACGAG 1 cut(s) 1017
Bst2UI CCWGG 5 cut(s) 117, 159, 192, 351, 926
Bst4CI ACNGT 4 cut(s) 11, 89, 760, 1134
Bst6I CTCTTC 1 cut(s) 703
BstACI GRCGYC 2 cut(s) 693, 886
BstC8I GCNNGC 8 cut(s) 221, 373, 459, 527, 548, 552, 765, 922
BstDEI CTNAG 3 cut(s) 129, 143, 231
BstDSI CCRYGG 2 cut(s) 10, 557
BstF5I GGATG 7 cut(s) 77, 166, 235, 262, 280, 346, 1035
BstFNI CGCG 2 cut(s) 477, 846
BstKTI GATC 6 cut(s) 319, 388, 475, 819, 826, 982
BstMAI GTCTC 4 cut(s) 9, 700, 938, 968
BstMBI GATC 6 cut(s) 316, 385, 472, 816, 823, 979
BstMCI CGRYCG 1 cut(s) 982
BstMWI GCNNNNNNNGC 5 cut(s) 130, 163, 463, 526, 543
BstNI CCWGG 5 cut(s) 117, 159, 192, 351, 926
BstSCI CCNGG 7 cut(s) 115, 157, 190, 224, 349, 674, 924
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 2 cut(s) 477, 846
BstV1I GCAGC 4 cut(s) 133, 145, 526, 728
BstV2I GAAGAC 2 cut(s) 330, 486
BstXI CCANNNNNNTGG 1 cut(s) 371
BtgI CCRYGG 2 cut(s) 10, 557
BtsCI GGATG 7 cut(s) 77, 166, 235, 262, 280, 346, 1035
BtsIMutI CAGTG 1 cut(s) 765
BtuMI TCGCGA 1 cut(s) 477
Cac8I GCNNGC 8 cut(s) 221, 373, 459, 527, 548, 552, 765, 922
CciI TCATGA 3 cut(s) 319, 388, 469
Cfr10I RCCGGY 3 cut(s) 219, 525, 635
Cfr13I GGNCC 4 cut(s) 161, 222, 374, 984
CseI GACGC 2 cut(s) 392, 875
Csp6I GTAC 2 cut(s) 859, 1045
CviAII CATG 8 cut(s) 320, 365, 389, 470, 521, 585, 730, 895
CviQI GTAC 2 cut(s) 859, 1045
DdeI CTNAG 3 cut(s) 129, 143, 231
DpnI GATC 6 cut(s) 318, 387, 474, 818, 825, 981
DpnII GATC 6 cut(s) 316, 385, 472, 816, 823, 979
EaeI YGGCCR 3 cut(s) 38, 497, 523
Eam1104I CTCTTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 703
EciI GGCGGA 1 cut(s) 691
Eco31I GGTCTC 1 cut(s) 968
Eco32I GATATC 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 161
EcoRII CCWGG 5 cut(s) 115, 157, 190, 349, 924
EcoRV GATATC 1 cut(s) 737
Esp3I CGTCTC 2 cut(s) 700, 938
FaeI CATG 8 cut(s) 323, 368, 392, 473, 524, 588, 733, 898
FaqI GGGAC 2 cut(s) 657, 1079
FatI CATG 8 cut(s) 319, 364, 388, 469, 520, 584, 729, 894
FauI CCCGC 4 cut(s) 224, 235, 471, 851
FauNDI CATATG 1 cut(s) 912
FbaI TGATCA 3 cut(s) 316, 385, 816
Fnu4HI GCNGC 5 cut(s) 122, 134, 515, 518, 717
FokI GGATG 7 cut(s) 64, 153, 222, 249, 267, 333, 1022
Fsp4HI GCNGC 5 cut(s) 122, 134, 515, 518, 717
FspBI CTAG 3 cut(s) 641, 921, 1086
GluI GCNGC 5 cut(s) 122, 134, 515, 518, 717
HapII CCGG 8 cut(s) 37, 220, 226, 239, 526, 636, 676, 1042
HgaI GACGC 2 cut(s) 392, 875
Hin1I GRCGYC 2 cut(s) 693, 886
Hin1II CATG 8 cut(s) 323, 368, 392, 473, 524, 588, 733, 898
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 1 cut(s) 679
HpaII CCGG 8 cut(s) 37, 220, 226, 239, 526, 636, 676, 1042
HphI GGTGA 3 cut(s) 26, 104, 766
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 7 cut(s) 146, 291, 339, 411, 684, 1013, 1031
Hpy188III TCNNGA 9 cut(s) 59, 320, 389, 470, 476, 591, 725, 961, 1094
Hpy8I GTNNAC 1 cut(s) 802
Hpy99I CGWCG 3 cut(s) 498, 695, 755
HpyAV CCTTC 4 cut(s) 386, 784, 880, 958
HpyCH4III ACNGT 4 cut(s) 11, 89, 760, 1134
HpyCH4IV ACGT 2 cut(s) 693, 753
HpyCH4V TGCA 5 cut(s) 550, 608, 878, 1060, 1122
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 163, 463, 526, 543
HpyF3I CTNAG 3 cut(s) 129, 143, 231
HpySE526I ACGT 2 cut(s) 693, 753
Hsp92I GRCGYC 2 cut(s) 693, 886
Hsp92II CATG 8 cut(s) 323, 368, 392, 473, 524, 588, 733, 898
KroI GCCGGC 2 cut(s) 219, 525
KroNI GCCGGC 2 cut(s) 221, 527
Ksp22I TGATCA 3 cut(s) 316, 385, 816
Kzo9I GATC 6 cut(s) 316, 385, 472, 816, 823, 979
LmnI GCTCC 1 cut(s) 593
Lsp1109I GCAGC 4 cut(s) 133, 145, 526, 728
LweI GCATC 4 cut(s) 175, 244, 427, 475
MaeI CTAG 3 cut(s) 641, 921, 1086
MaeII ACGT 2 cut(s) 693, 753
MaeIII GTNAC 3 cut(s) 14, 48, 754
MalI GATC 6 cut(s) 318, 387, 474, 818, 825, 981
MboI GATC 6 cut(s) 316, 385, 472, 816, 823, 979
MboII GAAGA 5 cut(s) 287, 335, 491, 690, 731
MhlI GDGCHC 2 cut(s) 1018, 1083
MluCI AATT 3 cut(s) 203, 609, 686
MlyI GAGTC 1 cut(s) 673
MroNI GCCGGC 2 cut(s) 219, 525
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 1 cut(s) 12
MspI CCGG 8 cut(s) 37, 220, 226, 239, 526, 636, 676, 1042
MspR9I CCNGG 7 cut(s) 117, 159, 192, 226, 351, 676, 926
MvaI CCWGG 5 cut(s) 117, 159, 192, 351, 926
MvnI CGCG 2 cut(s) 477, 846
MwoI GCNNNNNNNGC 5 cut(s) 130, 163, 463, 526, 543
NaeI GCCGGC 2 cut(s) 221, 527
NciI CCSGG 2 cut(s) 226, 676
NdeI CATATG 1 cut(s) 912
NdeII GATC 6 cut(s) 316, 385, 472, 816, 823, 979
NgoMIV GCCGGC 2 cut(s) 219, 525
NheI GCTAGC 1 cut(s) 920
NlaIII CATG 8 cut(s) 323, 368, 392, 473, 524, 588, 733, 898
NlaIV GGNNCC 4 cut(s) 99, 224, 456, 595
NmuCI GTSAC 2 cut(s) 14, 48
NruI TCGCGA 1 cut(s) 477
OliI CACNNNNGTG 1 cut(s) 12
PagI TCATGA 3 cut(s) 319, 388, 469
PasI CCCWGGG 1 cut(s) 116
PdiI GCCGGC 2 cut(s) 221, 527
PdmI GAANNNNTTC 1 cut(s) 66
PkrI GCNGC 5 cut(s) 123, 135, 516, 519, 718
Ple19I CGATCG 1 cut(s) 982
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Psp6I CCWGG 5 cut(s) 115, 157, 190, 349, 924
PspGI CCWGG 5 cut(s) 115, 157, 190, 349, 924
PspN4I GGNNCC 4 cut(s) 99, 224, 456, 595
PspPI GGNCC 4 cut(s) 161, 222, 374, 984
PvuI CGATCG 1 cut(s) 982
RruI TCGCGA 1 cut(s) 477
RsaI GTAC 2 cut(s) 860, 1046
RsaNI GTAC 2 cut(s) 859, 1045
RseI CAYNNNNRTG 1 cut(s) 12
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 5 cut(s) 122, 134, 515, 518, 717
Sau3AI GATC 6 cut(s) 316, 385, 472, 816, 823, 979
Sau96I GGNCC 4 cut(s) 161, 222, 374, 984
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 7 cut(s) 117, 159, 192, 226, 351, 676, 926
SduI GDGCHC 2 cut(s) 1018, 1083
SfaNI GCATC 4 cut(s) 175, 244, 427, 475
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
Sse9I AATT 3 cut(s) 203, 609, 686
SsiI CCGC 7 cut(s) 164, 217, 242, 464, 518, 702, 844
SspMI CTAG 3 cut(s) 641, 921, 1086
StyD4I CCNGG 7 cut(s) 115, 157, 190, 224, 349, 674, 924
TaaI ACNGT 4 cut(s) 11, 89, 760, 1134
TaiI ACGT 2 cut(s) 696, 756
TaqI TCGA 1 cut(s) 690
TaqII GACCGA 1 cut(s) 992
TasI AATT 3 cut(s) 203, 609, 686
TatI WGTACW 1 cut(s) 858
TauI GCSGC 1 cut(s) 520
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 2 cut(s) 14, 48
TseI GCWGC 4 cut(s) 121, 133, 514, 716
Tsp45I GTSAC 2 cut(s) 14, 48
TspDTI ATGAA 5 cut(s) 81, 251, 336, 405, 899
TspGWI ACGGA 2 cut(s) 36, 849
TspRI CASTG 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 3 cut(s) 641, 921, 1086
ZraI GACGTC 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.