AT1G13340

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012396
Physical Location & Seq
Reverse (-)
4569448 .. 4571229
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G13340.1

Sequence Viewer

Length: 1230 bp
ATGGGGAAGAAGCTTGACGCTTTGCTTGGTCGCAGTTTCAAGACCAACAAGTTCAAGTCTCTTATCACCTTAGCTCTCACAAGGCTCTCTATTCTCAAGAACCAGCGTCAAGCCAGACTCTCTCAAGCCATCTCTGATGTCACCGAGCTTCTTAAGCTCGGCCAACATGAGCACGCTTACCATAGAGTCGATCAAGTCGTCAAAGACCAAAACACTCTCGACGTTCTCTTCTTCATCCATGGCTACTTCACTCTCTGTCTCGATCGTATTCACCTCTTTGAACACAACAGAGATTGTCCTGAAGAGCTTCTAGAAGCTGTTTCGGGTTTGCTATTTGCAGCTTCTAGAATCGGTGAATTCCCTGAGCTTCAAGAGATTCGTAATGTTTTGATTTCGCGTTTTGGCAAAGATCTCGCGGCTCGGTCTATTGAGTTGCGCAGTAATTGTGGGGTTGATCCCAAAATAATTCAGAAGTTGTCGACAAGACCTCCACCGAAAGAAGTTAGAATGAAGGCTTTGAAGGAGATTGCTGCAGAGAATAACATTGTTCTGAAACTAGACCAAGCTTCTACGTCCACTGAGGGAACAACAAATATGCAGGGAACATCAGATGTTAGTAAGACTAAGCTAACAAGTAAAGATGGCAGAGGAGAAGGTTATGGTTTATCTGATTCGGTCAAGAGAGGAAAGAAGAAGTACAAAGATGTGGCTGATGCTGCACAAGCAGCGTTTGAGTCAGCAGCTCATGCAGCAGAGGCTGCACAAGCGGCTGTGGAGCTTTCTCAGTTTTCTCCTCGTGGACATGACAGTCCGGGTAACATTGGTGGGGAAAATTCATTTCATGGTTCTGAAAACAAAGAGTCAGAACAAGAACAAGAAGGTAATGATGATTCTTCAGAAGGGGAAGGGGATGTGATGTCAGAATCTAAAAGGTCGATGTCAGATTCAGAAGATATTATTGATGTCCCAGTGATGTCATTCAGAGAAGACCCTGTGAAGCTATTGGAGAAGGATACTATTATCTACGATAGTGAAGAAGAAACCCAACCTAGTGTTGAGACTATTACTATTAGTAAGTCTAAAGATGAGCAAAATCGTATGGTTGGTTCGAATAGAGCGGATACAAGACATGTGGACCACATGGTTCGTTCAATTGAAGATCCTATTATGCGTAAAGCAGGTTTGAAGGGTCCAGTTTCAGTCAGGACAAGACAGGTTCGTGGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

409

Amino Acids

45.4

Weight (kDa)

5.96

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 19 - 182 9.5e-49 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 807
Acc16I TGCGCA 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 1169
AccBSI CCGCTC 1 cut(s) 1118
AccI GTMKAC 1 cut(s) 479
AccII CGCG 2 cut(s) 397, 416
AciI CCGC 3 cut(s) 416, 767, 1118
AclWI GGATC 2 cut(s) 449, 1154
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 356, 832
AcuI CTGAAG 2 cut(s) 321, 879
AfaI GTAC 1 cut(s) 698
AflII CTTAAG 1 cut(s) 152
AflIII ACRYGT 1 cut(s) 1129
AgsI TTSAA 8 cut(s) 40, 55, 281, 371, 520, 1152, 1157, 1186
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 63, 263, 1052
AlwI GGATC 2 cut(s) 449, 1154
AlwNI CAGNNNCTG 1 cut(s) 758
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 7 cut(s) 338, 530, 716, 725, 740, 749, 758
ApoI RAATTY 2 cut(s) 356, 832
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 1135, 1190
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 4 cut(s) 58, 133, 263, 365
AsuII TTCGAA 1 cut(s) 1109
AvaII GGWCC 2 cut(s) 1135, 1190
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 993
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 7 cut(s) 350, 517, 703, 737, 745, 752, 761
BccI CCATC 2 cut(s) 137, 635
BcgI CGANNNNNNTGC 2 cut(s) 394, 428
BciVI GTATCC 3 cut(s) 1006, 1114, 1217
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 3 cut(s) 63, 263, 1052
BfaI CTAG 4 cut(s) 311, 345, 557, 1050
BfmI CTRYAG 1 cut(s) 531
BfrI CTTAAG 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 1169
BfuI GTATCC 3 cut(s) 1006, 1114, 1217
BglII AGATCT 1 cut(s) 409
BisI GCNGC 9 cut(s) 339, 417, 531, 717, 726, 741, 750, 759, 768
BlsI GCNGC 9 cut(s) 340, 418, 532, 718, 727, 742, 751, 760, 769
Bme1390I CCNGG 1 cut(s) 813
Bme18I GGWCC 2 cut(s) 1135, 1190
BmgT120I GGNCC 2 cut(s) 1135, 1190
BmiI GGNNCC 1 cut(s) 1191
BmrFI CCNGG 1 cut(s) 813
BmrI ACTGGG 1 cut(s) 962
BmsI GCATC 1 cut(s) 703
BmuI ACTGGG 1 cut(s) 962
BpiI GAAGAC 1 cut(s) 993
Bpu10I CCTNAGC 2 cut(s) 70, 363
Bpu14I TTCGAA 1 cut(s) 1109
BpuEI CTTGAG 2 cut(s) 80, 108
BpuMI CCSGG 1 cut(s) 813
BsaJI CCNNGG 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bse1I ACTGG 2 cut(s) 968, 1193
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 2 cut(s) 234, 916
BseMII CTCAG 3 cut(s) 354, 570, 797
BseNI ACTGG 2 cut(s) 968, 1193
BseRI GAGGAG 2 cut(s) 663, 783
BseXI GCAGC 7 cut(s) 350, 517, 703, 737, 745, 752, 761
BsgI GTGCAG 2 cut(s) 702, 744
Bsh1236I CGCG 2 cut(s) 397, 416
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 812
BslFI GGGAC 1 cut(s) 950
BsmAI GTCTC 3 cut(s) 63, 263, 1052
BsmFI GGGAC 1 cut(s) 950
BsnI GGCC 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 1109
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 5 cut(s) 190, 262, 409, 454, 1159
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 3 cut(s) 416, 767, 1118
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 3 cut(s) 355, 571, 796
BspFNI CGCG 2 cut(s) 397, 416
BspLI GGNNCC 1 cut(s) 1191
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 1 cut(s) 1169
BspPI GGATC 2 cut(s) 449, 1154
BspQI GCTCTTC 1 cut(s) 297
BspT104I TTCGAA 1 cut(s) 1109
BspTI CTTAAG 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 1118
BsrI ACTGG 2 cut(s) 968, 1193
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 5 cut(s) 190, 262, 409, 454, 1159
BssSI CACGAG 1 cut(s) 795
BssT1I CCWWGG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 795
Bst4CI ACNGT 1 cut(s) 809
Bst6I CTCTTC 2 cut(s) 233, 297
BstAFI CTTAAG 1 cut(s) 152
BstAPI GCANNNNNTGC 2 cut(s) 746, 758
BstBI TTCGAA 1 cut(s) 1109
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 5 cut(s) 70, 363, 579, 624, 783
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 2 cut(s) 234, 916
BstFNI CGCG 2 cut(s) 397, 416
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 5 cut(s) 193, 265, 412, 457, 1162
BstMAI GTCTC 3 cut(s) 63, 263, 1052
BstMBI GATC 5 cut(s) 190, 262, 409, 454, 1159
BstMCI CGRYCG 1 cut(s) 265
BstNSI RCATGY 1 cut(s) 1133
BstSCI CCNGG 1 cut(s) 811
BstSFI CTRYAG 1 cut(s) 531
BstUI CGCG 2 cut(s) 397, 416
BstV1I GCAGC 7 cut(s) 350, 517, 703, 737, 745, 752, 761
BstV2I GAAGAC 1 cut(s) 993
BstX2I RGATCY 2 cut(s) 409, 1159
BstYI RGATCY 2 cut(s) 409, 1159
BsuI GTATCC 3 cut(s) 1006, 1114, 1217
BsuRI GGCC 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 2 cut(s) 234, 916
BtsIMutI CAGTG 2 cut(s) 576, 975
BveI ACCTGC 1 cut(s) 1169
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 758
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 1135, 1190
CseI GACGC 2 cut(s) 26, 95
Csp6I GTAC 1 cut(s) 697
CspCI CAANNNNNGTGG 2 cut(s) 1113, 1148
CviAII CATG 7 cut(s) 167, 239, 746, 803, 842, 1130, 1141
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 5 cut(s) 70, 363, 579, 624, 783
DpnI GATC 5 cut(s) 192, 264, 411, 456, 1161
DpnII GATC 5 cut(s) 190, 262, 409, 454, 1159
DrdI GACNNNNNNGTC 1 cut(s) 807
DseDI GACNNNNNNGTC 1 cut(s) 807
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 2 cut(s) 233, 297
EarI CTCTTC 2 cut(s) 233, 297
Eco130I CCWWGG 1 cut(s) 238
Eco47I GGWCC 2 cut(s) 1135, 1190
Eco57I CTGAAG 2 cut(s) 321, 879
EcoRI GAATTC 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 7 cut(s) 170, 242, 749, 806, 845, 1133, 1144
FaqI GGGAC 1 cut(s) 950
FatI CATG 7 cut(s) 166, 238, 745, 802, 841, 1129, 1140
FblI GTMKAC 1 cut(s) 479
Fnu4HI GCNGC 9 cut(s) 339, 417, 531, 717, 726, 741, 750, 759, 768
FokI GGATG 2 cut(s) 221, 923
Fsp4HI GCNGC 9 cut(s) 339, 417, 531, 717, 726, 741, 750, 759, 768
FspBI CTAG 4 cut(s) 311, 345, 557, 1050
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 1 cut(s) 437
GluI GCNGC 9 cut(s) 339, 417, 531, 717, 726, 741, 750, 759, 768
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 812
HgaI GACGC 2 cut(s) 26, 95
HhaI GCGC 1 cut(s) 438
Hin1II CATG 7 cut(s) 170, 242, 749, 806, 845, 1133, 1144
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HindIII AAGCTT 2 cut(s) 11, 564
HpaII CCGG 1 cut(s) 812
HphI GGTGA 4 cut(s) 58, 133, 263, 365
Hpy166II GTNNAC 4 cut(s) 480, 576, 800, 1135
Hpy8I GTNNAC 4 cut(s) 480, 576, 800, 1135
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 8 cut(s) 505, 514, 647, 872, 893, 899, 1003, 1180
HpyCH4III ACNGT 1 cut(s) 809
HpyCH4IV ACGT 2 cut(s) 222, 572
HpyCH4V TGCA 6 cut(s) 338, 533, 598, 719, 749, 761
HpyF3I CTNAG 5 cut(s) 70, 363, 579, 624, 783
HpySE526I ACGT 2 cut(s) 222, 572
Hsp92II CATG 7 cut(s) 170, 242, 749, 806, 845, 1133, 1144
HspAI GCGC 1 cut(s) 436
Kzo9I GATC 5 cut(s) 190, 262, 409, 454, 1159
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 7 cut(s) 350, 517, 703, 737, 745, 752, 761
LweI GCATC 1 cut(s) 703
MaeI CTAG 4 cut(s) 311, 345, 557, 1050
MaeII ACGT 2 cut(s) 222, 572
MaeIII GTNAC 2 cut(s) 139, 815
MalI GATC 5 cut(s) 192, 264, 411, 456, 1161
MbiI CCGCTC 1 cut(s) 1118
MboI GATC 5 cut(s) 190, 262, 409, 454, 1159
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 2 cut(s) 409, 1159
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 5 cut(s) 356, 442, 465, 832, 1152
MlyI GAGTC 4 cut(s) 111, 195, 743, 869
MnlI CCTC 7 cut(s) 284, 498, 574, 641, 677, 748, 804
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 1 cut(s) 153
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 1 cut(s) 812
MspR9I CCNGG 1 cut(s) 813
MunI CAATTG 1 cut(s) 1152
MvnI CGCG 2 cut(s) 397, 416
NciI CCSGG 1 cut(s) 813
NcoI CCATGG 1 cut(s) 238
NdeII GATC 5 cut(s) 190, 262, 409, 454, 1159
NlaIII CATG 7 cut(s) 170, 242, 749, 806, 845, 1133, 1144
NlaIV GGNNCC 1 cut(s) 1191
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 139
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 1 cut(s) 1133
NspV TTCGAA 1 cut(s) 1109
PciI ACATGT 1 cut(s) 1129
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 6 cut(s) 348, 376, 671, 890, 923, 944
PkrI GCNGC 9 cut(s) 340, 418, 532, 718, 727, 742, 751, 760, 769
Ple19I CGATCG 1 cut(s) 265
PleI GAGTC 4 cut(s) 111, 194, 742, 868
PpsI GAGTC 4 cut(s) 111, 194, 742, 868
PscI ACATGT 1 cut(s) 1129
PspN4I GGNNCC 1 cut(s) 1191
PspPI GGNCC 2 cut(s) 1135, 1190
PsrI GAACNNNNNNTAC 2 cut(s) 1090, 1122
PstI CTGCAG 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 2 cut(s) 409, 1159
PvuI CGATCG 1 cut(s) 265
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
SalI GTCGAC 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 9 cut(s) 339, 417, 531, 717, 726, 741, 750, 759, 768
Sau3AI GATC 5 cut(s) 190, 262, 409, 454, 1159
Sau96I GGNCC 2 cut(s) 1135, 1190
SchI GAGTC 4 cut(s) 111, 195, 743, 869
ScrFI CCNGG 1 cut(s) 813
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 1 cut(s) 703
SfcI CTRYAG 1 cut(s) 531
SfuI TTCGAA 1 cut(s) 1109
SinI GGWCC 2 cut(s) 1135, 1190
SmlI CTYRAG 3 cut(s) 95, 123, 152
SmoI CTYRAG 3 cut(s) 95, 123, 152
Sse9I AATT 5 cut(s) 356, 442, 465, 832, 1152
SsiI CCGC 3 cut(s) 416, 767, 1118
SspMI CTAG 4 cut(s) 311, 345, 557, 1050
StyD4I CCNGG 1 cut(s) 811
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 809
TaiI ACGT 2 cut(s) 225, 575
TaqI TCGA 6 cut(s) 189, 219, 261, 479, 935, 1109
TaqII GACCGA 2 cut(s) 411, 664
TasI AATT 5 cut(s) 356, 442, 465, 832, 1152
TatI WGTACW 1 cut(s) 696
TauI GCSGC 2 cut(s) 419, 770
TfiI GAWTC 6 cut(s) 348, 376, 671, 890, 923, 944
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 2 cut(s) 583, 975
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 7 cut(s) 338, 530, 716, 725, 740, 749, 758
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 4 cut(s) 223, 524, 825, 830
TspRI CASTG 2 cut(s) 583, 975
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 1135, 1190
XapI RAATTY 2 cut(s) 356, 832
XbaI TCTAGA 2 cut(s) 310, 344
XceI RCATGY 1 cut(s) 1133
XmiI GTMKAC 1 cut(s) 479
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 4 cut(s) 311, 345, 557, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.