Prupe.1G308600_v2.0.a1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012396
Physical Location & Seq
Reverse (-)
30042941 .. 30045487
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G308600.1

Sequence Viewer

Length: 1227 bp
ATGGGAAGAAAGCTCGATGCTTTGCTCGGAAGGACTTACAAGACCTCCAAGCTCAAACCACTTTTGAATCTTGCTCTGTCTCGCCTTGCTGTCCTCAAGAACCTGCGCCAGGTCAAGTTCTCCCAAGCCCGCTCTGATGTTCTTCAGCTCCTCCAACTTGGTCACCATGAACGAGCTCTTCTTCGGGTGGAGCAGGTGATTAAGGAGCAGAATATGTTGGATGTGCTTGTCATGATAGAGCGTTACTGCAATCTCGTGATTGAAAGGGTTCACCTCATTGAACAAGAAAGAGTATGTCCTGATGAACTGAGGGAGGCAACGTCGAGTTTGCTTTATGCGGCTTCAAGATGTGGGGATTTCCCAGAGCTTCAAGAGATTCGTACGGTTCTCACCTATCGTTTTGGCAAGGAATTTGCTGCTCGTGCCATTGAAGTGCGCAACAATTGTGGAGTCAACCTTACGATGATGCAAAAACTGTCAACTAGGATGCCAGTTTTGGAGATCAGAGTGAAGGTGCTCAAAGAAATTGCTGCAGAGAACAGCATTGTTCTGCAGCTGGAGGAAACTACTTCTGTTTCCACTGAGGAGAAGTTGGATGTGAACAAGAATCAAAACCAGGCTGCACCAAACCCACCGGCCAGTTCAAGTGGCACGGCATCAGCGGATAATTTGCAGGCTTTCTCTCAAGAGACAGAAAAAGATGATAGGTTCTCTGGTTCACTTAAGAACAGGAAATACAAGGATGTAGCTGATGCTGCTCAAGCAGCTTTCGAGTCTGCAGCTTATGCGGCAGCAGCTGCAAGAGCAGCAGTGGAACTGTCTAGGTCTGAATCTCATGATCCTGATGATCAAAATAGTCCCGGTAGTAAACGAGGAAGGTTATCTGACAGATATGAGTCCTTTAAAAGTGAATCTGAATCACAAAATGAGCAAAATCGAGGTGAAGAATTGAAGAGGTCAACAACTTCTTCAAGTTTAGATTCAGATGGAGATGCCATAGTCCATGCCAGTCTTTCAGGGAAGGATATAGCGTTTGATGAGAGTGAGAGTGACAATGAACAAAGTGCCATGCCCTCATCTCATAAGCAAATTCCTTCAAGGTTTCAATCTGGGCTGAAGGTTGAGTCAGCACATGCTGCTGGAGGGTCTGGAAGGCAAAGCCCACCAAGATTGAACATAGAGAAGGCACCATTCTCTCTGAGGACAAGAGGGGTGCGTGGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

409

Amino Acids

45.2

Weight (kDa)

7.07

Isoelectric Point (pI)

56.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 184
Acc16I TGCGCA 1 cut(s) 437
Acc36I ACCTGC 2 cut(s) 111, 184
AccB1I GGYRCC 1 cut(s) 1186
AccBSI CCGCTC 1 cut(s) 132
AciI CCGC 4 cut(s) 130, 338, 662, 788
AclWI GGATC 1 cut(s) 833
AcoI YGGCCR 1 cut(s) 636
AcsI RAATTY 2 cut(s) 410, 1089
AcuI CTGAAG 2 cut(s) 128, 1136
AfaI GTAC 2 cut(s) 382, 1223
AfiI CCNNNNNNNGG 1 cut(s) 109
AflII CTTAAG 1 cut(s) 722
AjnI CCWGG 2 cut(s) 108, 615
Alw21I GWGCWC 2 cut(s) 178, 519
Alw26I GTCTC 2 cut(s) 84, 683
AlwI GGATC 1 cut(s) 833
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 1 cut(s) 636
ApoI RAATTY 2 cut(s) 410, 1089
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 108, 438
AsuC2I CCSGG 1 cut(s) 861
AsuHPI GGTGA 5 cut(s) 155, 208, 263, 382, 953
BanI GGYRCC 1 cut(s) 1186
BanII GRGCYC 1 cut(s) 178
BauI CACGAG 2 cut(s) 254, 420
Bbv12I GWGCWC 2 cut(s) 178, 519
BccI CCATC 1 cut(s) 980
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 2 cut(s) 110, 617
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 1 cut(s) 861
BcoDI GTCTC 2 cut(s) 84, 683
BfaI CTAG 2 cut(s) 483, 822
BfmI CTRYAG 3 cut(s) 531, 551, 777
BfrI CTTAAG 1 cut(s) 722
BfuAI ACCTGC 2 cut(s) 111, 184
Bme1390I CCNGG 3 cut(s) 110, 617, 861
BmiI GGNNCC 1 cut(s) 1188
BmrFI CCNGG 3 cut(s) 110, 617, 861
BmsI GCATC 6 cut(s) 7, 456, 477, 665, 742, 982
BpmI CTGGAG 2 cut(s) 578, 1161
BpuEI CTTGAG 3 cut(s) 80, 669, 744
BpuMI CCSGG 1 cut(s) 861
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 3 cut(s) 491, 639, 1008
BseBI CCWGG 2 cut(s) 110, 617
BseGI GGATG 4 cut(s) 226, 492, 601, 748
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 3 cut(s) 299, 573, 1190
BseNI ACTGG 3 cut(s) 491, 639, 1008
BseRI GAGGAG 2 cut(s) 140, 599
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 1 cut(s) 638
BshNI GGYRCC 1 cut(s) 1186
BsiHKAI GWGCWC 2 cut(s) 178, 519
BsiSI CCGG 2 cut(s) 635, 861
BsiWI CGTACG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 843
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 2 cut(s) 84, 683
BsmFI GGGAC 1 cut(s) 843
BsnI GGCC 1 cut(s) 638
Bsp1286I GDGCHC 2 cut(s) 178, 519
Bsp143I GATC 3 cut(s) 501, 838, 847
BspACI CCGC 4 cut(s) 130, 338, 662, 788
BspANI GGCC 1 cut(s) 638
BspCNI CTCAG 3 cut(s) 300, 574, 1191
BspHI TCATGA 2 cut(s) 231, 835
BspLI GGNNCC 1 cut(s) 1188
BspMAI CTGCAG 3 cut(s) 535, 555, 781
BspMI ACCTGC 2 cut(s) 111, 184
BspPI GGATC 1 cut(s) 833
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 1186
BspTI CTTAAG 1 cut(s) 722
BsrBI CCGCTC 1 cut(s) 132
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 3 cut(s) 491, 639, 1008
BssAI RCCGGY 1 cut(s) 634
BssMI GATC 3 cut(s) 501, 838, 847
BssSI CACGAG 2 cut(s) 254, 420
Bst2BI CACGAG 2 cut(s) 254, 420
Bst2UI CCWGG 2 cut(s) 110, 617
Bst4CI ACNGT 3 cut(s) 385, 477, 819
Bst6I CTCTTC 2 cut(s) 183, 947
BstAFI CTTAAG 1 cut(s) 722
BstAPI GCANNNNNTGC 3 cut(s) 785, 797, 1136
BstC8I GCNNGC 2 cut(s) 130, 675
BstDEI CTNAG 3 cut(s) 308, 582, 1199
BstEII GGTNACC 1 cut(s) 161
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 4 cut(s) 226, 492, 601, 748
BstHHI GCGC 2 cut(s) 108, 438
BstKTI GATC 3 cut(s) 504, 841, 850
BstMAI GTCTC 2 cut(s) 84, 683
BstMBI GATC 3 cut(s) 501, 838, 847
BstNI CCWGG 2 cut(s) 110, 617
BstNSI RCATGY 1 cut(s) 1136
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 108, 615, 859
BstSFI CTRYAG 3 cut(s) 531, 551, 777
BsuRI GGCC 1 cut(s) 638
BtsCI GGATG 4 cut(s) 226, 492, 601, 748
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 2 cut(s) 579, 816
BveI ACCTGC 2 cut(s) 111, 184
Cac8I GCNNGC 2 cut(s) 130, 675
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 2 cut(s) 231, 835
CfoI GCGC 2 cut(s) 108, 438
Cfr10I RCCGGY 1 cut(s) 634
Csp6I GTAC 2 cut(s) 381, 1222
CspCI CAANNNNNGTGG 4 cut(s) 427, 462, 1152, 1187
CviAII CATG 6 cut(s) 167, 232, 836, 1004, 1069, 1133
CviQI GTAC 2 cut(s) 381, 1222
DdeI CTNAG 3 cut(s) 308, 582, 1199
DpnI GATC 3 cut(s) 503, 840, 849
DpnII GATC 3 cut(s) 501, 838, 847
DraI TTTAAA 1 cut(s) 904
EaeI YGGCCR 1 cut(s) 636
Eam1104I CTCTTC 2 cut(s) 183, 947
EarI CTCTTC 2 cut(s) 183, 947
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 2 cut(s) 128, 1136
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 108, 615
EcoT38I GRGCYC 1 cut(s) 178
FaeI CATG 6 cut(s) 170, 235, 839, 1007, 1072, 1136
FaqI GGGAC 1 cut(s) 843
FatI CATG 6 cut(s) 166, 231, 835, 1003, 1068, 1132
FauI CCCGC 1 cut(s) 137
FbaI TGATCA 1 cut(s) 847
FokI GGATG 4 cut(s) 233, 499, 608, 755
FriOI GRGCYC 1 cut(s) 178
FspBI CTAG 2 cut(s) 483, 822
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 2 cut(s) 107, 437
GsuI CTGGAG 2 cut(s) 578, 1161
HaeIII GGCC 1 cut(s) 638
HapII CCGG 2 cut(s) 635, 861
HhaI GCGC 2 cut(s) 108, 438
Hin1II CATG 6 cut(s) 170, 235, 839, 1007, 1072, 1136
Hin6I GCGC 2 cut(s) 106, 436
HinP1I GCGC 2 cut(s) 106, 436
HincII GTYRAC 3 cut(s) 454, 480, 960
HindII GTYRAC 3 cut(s) 454, 480, 960
HpaII CCGG 2 cut(s) 635, 861
HphI GGTGA 5 cut(s) 155, 208, 263, 382, 953
Hpy166II GTNNAC 7 cut(s) 271, 454, 480, 601, 719, 869, 960
Hpy188I TCNGA 8 cut(s) 29, 136, 506, 829, 886, 916, 985, 1200
Hpy8I GTNNAC 7 cut(s) 271, 454, 480, 601, 719, 869, 960
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 8 cut(s) 24, 505, 870, 1015, 1104, 1111, 1146, 1177
HpyCH4III ACNGT 3 cut(s) 385, 477, 819
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 8 cut(s) 249, 469, 533, 553, 623, 673, 779, 800
HpyF3I CTNAG 3 cut(s) 308, 582, 1199
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 6 cut(s) 170, 235, 839, 1007, 1072, 1136
HspAI GCGC 2 cut(s) 106, 436
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 3 cut(s) 501, 838, 847
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 3 cut(s) 153, 190, 205
LweI GCATC 6 cut(s) 7, 456, 477, 665, 742, 982
MaeI CTAG 2 cut(s) 483, 822
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 3 cut(s) 161, 242, 1049
MalI GATC 3 cut(s) 503, 840, 849
MbiI CCGCTC 1 cut(s) 132
MboI GATC 3 cut(s) 501, 838, 847
MboII GAAGA 7 cut(s) 18, 134, 170, 173, 956, 960, 964
MfeI CAATTG 1 cut(s) 442
MhlI GDGCHC 2 cut(s) 178, 519
MluCI AATT 6 cut(s) 410, 442, 525, 667, 947, 1089
MlyI GAGTC 4 cut(s) 459, 782, 905, 1133
MmeI TCCRAC 3 cut(s) 178, 198, 573
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 3 cut(s) 201, 723, 903
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 3 cut(s) 556, 662, 797
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 2 cut(s) 635, 861
MspR9I CCNGG 3 cut(s) 110, 617, 861
MunI CAATTG 1 cut(s) 442
MvaI CCWGG 2 cut(s) 110, 617
NciI CCSGG 1 cut(s) 861
NdeII GATC 3 cut(s) 501, 838, 847
NlaIII CATG 6 cut(s) 170, 235, 839, 1007, 1072, 1136
NlaIV GGNNCC 1 cut(s) 1188
NmuCI GTSAC 2 cut(s) 161, 1049
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 1 cut(s) 1136
PagI TCATGA 2 cut(s) 231, 835
PaqCI CACCTGC 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 7 cut(s) 67, 376, 607, 830, 911, 917, 980
Pfl23II CGTACG 1 cut(s) 380
PleI GAGTC 4 cut(s) 458, 781, 904, 1132
PpsI GAGTC 4 cut(s) 458, 781, 904, 1132
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 108, 615
PspEI GGTNACC 1 cut(s) 161
PspGI CCWGG 2 cut(s) 108, 615
PspLI CGTACG 1 cut(s) 380
PspN4I GGNNCC 1 cut(s) 1188
PsrI GAACNNNNNNTAC 2 cut(s) 719, 751
PstI CTGCAG 3 cut(s) 535, 555, 781
PstNI CAGNNNCTG 1 cut(s) 797
PvuII CAGCTG 2 cut(s) 556, 797
RsaI GTAC 2 cut(s) 382, 1223
RsaNI GTAC 2 cut(s) 381, 1222
RseI CAYNNNNRTG 1 cut(s) 431
SacI GAGCTC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 3 cut(s) 201, 723, 903
Sau3AI GATC 3 cut(s) 501, 838, 847
SchI GAGTC 4 cut(s) 459, 782, 905, 1133
ScrFI CCNGG 3 cut(s) 110, 617, 861
SduI GDGCHC 2 cut(s) 178, 519
SfaNI GCATC 6 cut(s) 7, 456, 477, 665, 742, 982
SfcI CTRYAG 3 cut(s) 531, 551, 777
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 4 cut(s) 95, 684, 722, 759
SmoI CTYRAG 4 cut(s) 95, 684, 722, 759
Sse9I AATT 6 cut(s) 410, 442, 525, 667, 947, 1089
SsiI CCGC 4 cut(s) 130, 338, 662, 788
SspMI CTAG 2 cut(s) 483, 822
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 3 cut(s) 108, 615, 859
TaaI ACNGT 3 cut(s) 385, 477, 819
TaiI ACGT 1 cut(s) 323
TaqI TCGA 4 cut(s) 15, 323, 771, 937
TasI AATT 6 cut(s) 410, 442, 525, 667, 947, 1089
TauI GCSGC 2 cut(s) 341, 791
TfiI GAWTC 7 cut(s) 67, 376, 607, 830, 911, 917, 980
Tru1I TTAA 3 cut(s) 201, 723, 903
Tru9I TTAA 3 cut(s) 201, 723, 903
TscAI CASTG 2 cut(s) 586, 816
TseFI GTSAC 2 cut(s) 161, 1049
Tsp45I GTSAC 2 cut(s) 161, 1049
TspDTI ATGAA 3 cut(s) 183, 318, 1071
TspRI CASTG 2 cut(s) 586, 816
Vha464I CTTAAG 1 cut(s) 722
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 2 cut(s) 410, 1089
XceI RCATGY 1 cut(s) 1136
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 2 cut(s) 483, 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.