pycom13g03850

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012396
Physical Location & Seq
Forward (+)
2571049 .. 2571705
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03850.1

Sequence Viewer

Length: 657 bp
ATGCAGGATAAATCGGATGTGAACAAGGCCGGTTCAAGTGGCACGGGATCAGCAGATAATGTGCAGGCTTCTGCAGAAGAGACGGGAAATGATGATAGGTTCTCTGGTTCGATTAAGCCAAGGAAATACAAGGATGTAGCTGATGCAGCTCAAGCAGCTTTCGAGTCTGCAGCTTACGCAGCAGATGCTGCTAGAGCAGCAGTGCAACTCTCTAGGTCTGAATCTCAAGATCCTGATGATCAAAATAGTCCCGGAAGTAAACGAGGAAAGTTATCTGACAGATATGAATCCTTTAAAAGTCTATCTGAATCGCAGCATGAGCAAAATCGAGGTGAATTAAAGAGGTCGACATCTTCTTCAAGTTTAGATTCAGATGGAGATGAGGTGATTCCAATGTCCTCAGATGCCGTATGGCAGGCCGATCTTTTAGGGAAGGATATAGTTTTTGATGAGAGTGAAAGCGACAACGAACCAAATACAACGCCCTCGTCTCAGAACCAAATTCCTTTGAGGTTTCGATCTGGCTTGAAGGCGGAGTCAAGGCTTGAATATCCTGCAGCACATGTCACTGGAGGATCTGGAAGGCAAAGCCCACCGCGATTAAACATAGAGAAGGGACCGTTCTCTCTGAGGACTAGACGGGTGCGTGGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

219

Amino Acids

23.54

Weight (kDa)

5.12

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 347
AccII CGCG 1 cut(s) 598
AciI CCGC 2 cut(s) 533, 596
AclWI GGATC 3 cut(s) 55, 224, 583
AcsI RAATTY 1 cut(s) 501
AflIII ACRYGT 1 cut(s) 562
AgsI TTSAA 4 cut(s) 36, 360, 529, 548
AluBI AGCT 4 cut(s) 140, 149, 158, 173
AluI AGCT 4 cut(s) 140, 149, 158, 173
Alw26I GTCTC 2 cut(s) 74, 495
AlwI GGATC 3 cut(s) 55, 224, 583
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 2 cut(s) 27, 417
ApeKI GCWGC 8 cut(s) 146, 155, 170, 179, 188, 197, 313, 557
ApoI RAATTY 1 cut(s) 501
AspS9I GGNCC 1 cut(s) 617
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 344, 397
AvaII GGWCC 1 cut(s) 617
BbvI GCAGC 8 cut(s) 158, 167, 175, 182, 191, 209, 325, 569
BccI CCATC 1 cut(s) 368
BceAI ACGGC 1 cut(s) 392
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 74, 495
BfaI CTAG 3 cut(s) 192, 213, 636
BfmI CTRYAG 3 cut(s) 72, 168, 555
BisI GCNGC 8 cut(s) 147, 156, 171, 180, 189, 198, 314, 558
BlsI GCNGC 8 cut(s) 148, 157, 172, 181, 190, 199, 315, 559
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 1 cut(s) 618
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 3 cut(s) 133, 175, 394
BpmI CTGGAG 1 cut(s) 591
BpuEI CTTGAG 2 cut(s) 135, 210
BpuMI CCSGG 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 119
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 286
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 2 cut(s) 22, 139
BseJI GATNNNNATC 1 cut(s) 286
BseMII CTCAG 3 cut(s) 414, 506, 620
BseNI ACTGG 1 cut(s) 574
BseXI GCAGC 8 cut(s) 158, 167, 175, 182, 191, 209, 325, 569
BsgI GTGCAG 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 598
BshFI GGCC 2 cut(s) 29, 419
BsiSI CCGG 2 cut(s) 30, 252
BslFI GGGAC 2 cut(s) 234, 630
BsmAI GTCTC 2 cut(s) 74, 495
BsmBI CGTCTC 2 cut(s) 74, 495
BsmFI GGGAC 2 cut(s) 234, 630
BsnI GGCC 2 cut(s) 29, 419
Bsp143I GATC 6 cut(s) 47, 229, 238, 421, 518, 575
BspACI CCGC 2 cut(s) 533, 596
BspANI GGCC 2 cut(s) 29, 419
BspCNI CTCAG 3 cut(s) 413, 505, 621
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 618
BspMAI CTGCAG 3 cut(s) 76, 172, 559
BspPI GGATC 3 cut(s) 55, 224, 583
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 6 cut(s) 47, 229, 238, 421, 518, 575
BssT1I CCWWGG 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 72
BstAPI GCANNNNNTGC 2 cut(s) 185, 188
BstC8I GCNNGC 2 cut(s) 66, 417
BstDEI CTNAG 3 cut(s) 400, 492, 629
BstF5I GGATG 2 cut(s) 22, 139
BstFNI CGCG 1 cut(s) 598
BstKTI GATC 6 cut(s) 50, 232, 241, 424, 521, 578
BstMAI GTCTC 2 cut(s) 74, 495
BstMBI GATC 6 cut(s) 47, 229, 238, 421, 518, 575
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 3 cut(s) 72, 168, 555
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 8 cut(s) 158, 167, 175, 182, 191, 209, 325, 569
BstX2I RGATCY 2 cut(s) 229, 575
BstYI RGATCY 2 cut(s) 229, 575
BsuRI GGCC 2 cut(s) 29, 419
BtsCI GGATG 2 cut(s) 22, 139
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 2 cut(s) 207, 567
Cac8I GCNNGC 2 cut(s) 66, 417
CaiI CAGNNNCTG 1 cut(s) 188
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 617
CviAII CATG 2 cut(s) 317, 563
DdeI CTNAG 3 cut(s) 400, 492, 629
DpnI GATC 6 cut(s) 49, 231, 240, 423, 520, 577
DpnII GATC 6 cut(s) 47, 229, 238, 421, 518, 575
DraI TTTAAA 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
EciI GGCGGA 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 617
EcoT14I CCWWGG 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 119
Esp3I CGTCTC 2 cut(s) 74, 495
FaeI CATG 2 cut(s) 320, 566
FaiI YATR 6 cut(s) 285, 318, 412, 440, 564, 608
FaqI GGGAC 2 cut(s) 234, 630
FatI CATG 2 cut(s) 316, 562
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 8 cut(s) 147, 156, 171, 180, 189, 198, 314, 558
FokI GGATG 2 cut(s) 29, 146
Fsp4HI GCNGC 8 cut(s) 147, 156, 171, 180, 189, 198, 314, 558
FspBI CTAG 3 cut(s) 192, 213, 636
GluI GCNGC 8 cut(s) 147, 156, 171, 180, 189, 198, 314, 558
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 2 cut(s) 29, 419
HapII CCGG 2 cut(s) 30, 252
Hin1II CATG 2 cut(s) 320, 566
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 7 cut(s) 164, 221, 287, 308, 368, 388, 536
HpaII CCGG 2 cut(s) 30, 252
HphI GGTGA 2 cut(s) 344, 397
Hpy166II GTNNAC 3 cut(s) 22, 260, 348
Hpy188I TCNGA 9 cut(s) 16, 220, 277, 307, 373, 403, 495, 630, 656
Hpy188III TCNNGA 3 cut(s) 227, 233, 579
Hpy8I GTNNAC 3 cut(s) 22, 260, 348
HpyAV CCTTC 4 cut(s) 427, 523, 576, 607
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4V TGCA 7 cut(s) 4, 64, 74, 146, 170, 205, 557
HpyF3I CTNAG 3 cut(s) 400, 492, 629
Hsp92II CATG 2 cut(s) 320, 566
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 6 cut(s) 47, 229, 238, 421, 518, 575
Lsp1109I GCAGC 8 cut(s) 158, 167, 175, 182, 191, 209, 325, 569
LweI GCATC 3 cut(s) 133, 175, 394
MaeI CTAG 3 cut(s) 192, 213, 636
MaeIII GTNAC 1 cut(s) 565
MalI GATC 6 cut(s) 49, 231, 240, 423, 520, 577
MboI GATC 6 cut(s) 47, 229, 238, 421, 518, 575
MboII GAAGA 3 cut(s) 89, 345, 348
MflI RGATCY 2 cut(s) 229, 575
MluCI AATT 2 cut(s) 335, 501
MlyI GAGTC 2 cut(s) 173, 545
MnlI CCTC 9 cut(s) 257, 323, 336, 376, 409, 496, 504, 566, 624
MseI TTAA 4 cut(s) 114, 294, 338, 602
MspI CCGG 2 cut(s) 30, 252
MspR9I CCNGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 598
NciI CCSGG 1 cut(s) 252
NdeII GATC 6 cut(s) 47, 229, 238, 421, 518, 575
NlaIII CATG 2 cut(s) 320, 566
NlaIV GGNNCC 1 cut(s) 618
NmuCI GTSAC 1 cut(s) 565
NspI RCATGY 1 cut(s) 566
PciI ACATGT 1 cut(s) 562
PfeI GAWTC 5 cut(s) 221, 287, 308, 368, 388
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 8 cut(s) 148, 157, 172, 181, 190, 199, 315, 559
PleI GAGTC 2 cut(s) 172, 544
PpsI GAGTC 2 cut(s) 172, 544
PscI ACATGT 1 cut(s) 562
PspN4I GGNNCC 1 cut(s) 618
PspPI GGNCC 1 cut(s) 617
PstI CTGCAG 3 cut(s) 76, 172, 559
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 229, 575
SalI GTCGAC 1 cut(s) 346
SaqAI TTAA 4 cut(s) 114, 294, 338, 602
SatI GCNGC 8 cut(s) 147, 156, 171, 180, 189, 198, 314, 558
Sau3AI GATC 6 cut(s) 47, 229, 238, 421, 518, 575
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 2 cut(s) 173, 545
ScrFI CCNGG 1 cut(s) 252
SfaNI GCATC 3 cut(s) 133, 175, 394
SfcI CTRYAG 3 cut(s) 72, 168, 555
SinI GGWCC 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 150, 225
SmoI CTYRAG 2 cut(s) 150, 225
Sse9I AATT 2 cut(s) 335, 501
SsiI CCGC 2 cut(s) 533, 596
SspMI CTAG 3 cut(s) 192, 213, 636
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 621
TaqI TCGA 5 cut(s) 110, 162, 328, 347, 517
TasI AATT 2 cut(s) 335, 501
TfiI GAWTC 5 cut(s) 221, 287, 308, 368, 388
Tru1I TTAA 4 cut(s) 114, 294, 338, 602
Tru9I TTAA 4 cut(s) 114, 294, 338, 602
TscAI CASTG 2 cut(s) 207, 574
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 8 cut(s) 146, 155, 170, 179, 188, 197, 313, 557
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 1 cut(s) 300
TspRI CASTG 2 cut(s) 207, 574
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 1 cut(s) 501
XceI RCATGY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 347
XspI CTAG 3 cut(s) 192, 213, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.