MD16G1044700.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012396
Physical Location & Seq
Forward (+)
3159997 .. 3162048
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1044700.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGGAAGAAAGCTCGATGCTTTGCTTGGAAGGAGTCACAAGACCTCCGCCAAGTTCAAACCAGTTTTGAACCTCGCTCTCTCCCGCCTCGCCGTTCTCAAGAAACAGCGCCAGGTCAAGTGCTCTCAGGCTCGATCCGACGTCGTCCAGCTCCTCCAGCAGGGTCACCAAGATCGAGCTCTTCTTCGCGTGGAGCATGTGATCAAGGAGCAGAATATGTTGGACGTGTTTGTCATGATCGAGGGTTTTTGCAACCTCGTGATCGAAAGGGTTCATCTCATTGAACAAGAAAGGGAATGTCCTGATGAACTGAAGGAGGCAACATCGAGTTTGCTTTATGCGGCTTCAAGATGCGGGGATTTTCCGGAGCTTCAAGAGATTCGCGCGGTTCTCACTGCTCGTTTTGGCAAGGAATTTGCCGCTCGTGCCATCGAAGTGCGCAACAATTGTGGAGTCAACCTCACGATGATGCAAAAACTGTCAACTAGGATGCCCGTTTTGGAGGTCAGAATGAAGGTGCTCAAAGAAATTGCTGCAGAGAATAACATTGTTCTGACGCTGGAAGAAACGCCTTCCGTTTCCACTGAGGACAAATCGGATGCGAACAAGGCCAGTTCAAGTGGCACGGGACCAGCGGATAATGTGCAGGCTTCTGCAGAAGAGACCGGAAGAGATGATAGGTTCTCTGGTTCGATTAAGCCAAGGAAATACAAGGATGTAGCTGATGCTGCGCAAGCAGCTTTCGAGTCTGCAGCTTATGCAGCAGATGCTGCAAAAGCAGCAGTGCGACTCTCTAGGTCTGAATCTCATGATCCCGGTGATCAAAATAGTCCCGGAAGTAAACGAGGGAAGCTATCTGACAGATACGAGTCCTTTAAAACTGAATCAGAATCGCAGAGTGAGCAAAATCAAGGTGAATCAAAGAGGTCAACATCTTCTTTAAGTTTAGATTCAGATGGAGATGAGGTGATTCCAATGTCCTCAGATGCCGCAGGGCAGGCCAATCTTTTAGGGAAGGATATAGTTTTTGATGAGAGTGAAAGCGACAGCGAACCAAATACACCGCCCTCGTCTCATAAGCCAATTCCTTCGAGGTTTCAATCCGGCTTGAAGGCAGAGTCAAGGCTTGAATATCCTGCAGTACATGTCGCTGGAGGATCTGCAAAGCAAAGCCCACCGCGATTAAACATTGAGAAGGGACCATTCTCTCTGAGGACTCGACGGGTGCGTGGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

413

Amino Acids

45.15

Weight (kDa)

7.07

Isoelectric Point (pI)

58.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 20 - 183 1.4e-55 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 2 cut(s) 440, 732
AccBSI CCGCTC 1 cut(s) 422
AccII CGCG 4 cut(s) 189, 384, 386, 1180
AccIII TCCGGA 1 cut(s) 364
AclWI GGATC 3 cut(s) 129, 806, 1165
AcsI RAATTY 1 cut(s) 413
AcuI CTGAAG 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 1 cut(s) 1143
AfiI CCNNNNNNNGG 1 cut(s) 160
AflIII ACRYGT 2 cut(s) 225, 1144
AgsI TTSAA 9 cut(s) 58, 70, 284, 348, 374, 618, 1100, 1111, 1130
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 8 cut(s) 13, 151, 179, 370, 722, 740, 755, 853
AluI AGCT 8 cut(s) 13, 151, 179, 370, 722, 740, 755, 853
Alw21I GWGCWC 3 cut(s) 125, 181, 522
Alw26I GTCTC 2 cut(s) 656, 1077
AlwI GGATC 3 cut(s) 129, 806, 1165
AlwNI CAGNNNCTG 1 cut(s) 770
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 2 cut(s) 609, 999
ApeKI GCWGC 7 cut(s) 533, 728, 737, 752, 761, 770, 779
ApoI RAATTY 1 cut(s) 413
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 4 cut(s) 111, 386, 441, 733
AspS9I GGNCC 2 cut(s) 629, 1199
AsuC2I CCSGG 2 cut(s) 816, 834
AsuHPI GGTGA 4 cut(s) 158, 830, 926, 979
AvaII GGWCC 2 cut(s) 629, 1199
BanII GRGCYC 1 cut(s) 181
BauI CACGAG 2 cut(s) 257, 423
Bbv12I GWGCWC 3 cut(s) 125, 181, 522
BbvI GCAGC 7 cut(s) 520, 715, 749, 757, 764, 773, 791
BccI CCATC 2 cut(s) 437, 950
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 2 cut(s) 201, 820
BcnI CCSGG 2 cut(s) 816, 834
BcoDI GTCTC 2 cut(s) 656, 1077
BfaI CTAG 2 cut(s) 486, 795
BfmI CTRYAG 4 cut(s) 534, 654, 750, 1137
BfoI RGCGCY 1 cut(s) 112
Bme1390I CCNGG 3 cut(s) 113, 816, 834
Bme18I GGWCC 2 cut(s) 629, 1199
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 629, 1199
BmiI GGNNCC 2 cut(s) 630, 1200
BmrFI CCNGG 3 cut(s) 113, 816, 834
BmsI GCATC 8 cut(s) 7, 341, 459, 480, 589, 715, 757, 976
BplI GAGNNNNNCTC 2 cut(s) 444, 476
BpmI CTGGAG 2 cut(s) 140, 1173
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 816, 834
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 701
BsaWI WCCGGW 2 cut(s) 364, 665
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 951, 981
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 62, 612
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 3 cut(s) 495, 604, 721
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 4 cut(s) 140, 576, 996, 1202
BseNI ACTGG 2 cut(s) 62, 612
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 7 cut(s) 520, 715, 749, 757, 764, 773, 791
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 4 cut(s) 189, 384, 386, 1180
BshFI GGCC 2 cut(s) 611, 1001
BsiHKAI GWGCWC 3 cut(s) 125, 181, 522
BsiSI CCGG 5 cut(s) 365, 666, 816, 834, 1104
BslFI GGGAC 3 cut(s) 642, 816, 1212
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 656, 1077
BsmBI CGTCTC 1 cut(s) 1077
BsmFI GGGAC 3 cut(s) 642, 816, 1212
BsnI GGCC 2 cut(s) 611, 1001
Bso31I GGTCTC 1 cut(s) 656
Bsp1286I GDGCHC 3 cut(s) 125, 181, 522
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
BspANI GGCC 2 cut(s) 611, 1001
BspCNI CTCAG 4 cut(s) 139, 577, 995, 1203
BspEI TCCGGA 1 cut(s) 364
BspFNI CGCG 4 cut(s) 189, 384, 386, 1180
BspHI TCATGA 2 cut(s) 234, 808
BspLI GGNNCC 2 cut(s) 630, 1200
BspMAI CTGCAG 4 cut(s) 538, 658, 754, 1141
BspPI GGATC 3 cut(s) 129, 806, 1165
BspQI GCTCTTC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 422
BsrI ACTGG 2 cut(s) 62, 612
BssECI CCNNGG 1 cut(s) 701
BssMI GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 2 cut(s) 257, 423
BssT1I CCWWGG 1 cut(s) 701
Bst2BI CACGAG 2 cut(s) 257, 423
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 3 cut(s) 186, 654, 664
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 3 cut(s) 758, 767, 770
BstC8I GCNNGC 3 cut(s) 648, 735, 999
BstDEI CTNAG 4 cut(s) 126, 585, 982, 1211
BstEII GGTNACC 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 3 cut(s) 495, 604, 721
BstFNI CGCG 4 cut(s) 189, 384, 386, 1180
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 4 cut(s) 111, 386, 441, 733
BstKTI GATC 8 cut(s) 137, 175, 204, 240, 264, 814, 823, 1160
BstMAI GTCTC 2 cut(s) 656, 1077
BstMBI GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 200, 1148
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 3 cut(s) 111, 814, 832
BstSFI CTRYAG 4 cut(s) 534, 654, 750, 1137
BstUI CGCG 4 cut(s) 189, 384, 386, 1180
BstV1I GCAGC 7 cut(s) 520, 715, 749, 757, 764, 773, 791
BstX2I RGATCY 1 cut(s) 1157
BstYI RGATCY 1 cut(s) 1157
BsuRI GGCC 2 cut(s) 611, 1001
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 3 cut(s) 495, 604, 721
BtsI GCAGTG 2 cut(s) 393, 789
BtsIMutI CAGTG 3 cut(s) 393, 582, 789
Cac8I GCNNGC 3 cut(s) 648, 735, 999
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 2 cut(s) 234, 808
CfoI GCGC 4 cut(s) 111, 386, 441, 733
Cfr13I GGNCC 2 cut(s) 629, 1199
CseI GACGC 1 cut(s) 565
Csp6I GTAC 1 cut(s) 1142
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 4 cut(s) 197, 235, 809, 1145
CviQI GTAC 1 cut(s) 1142
DdeI CTNAG 4 cut(s) 126, 585, 982, 1211
DpnI GATC 8 cut(s) 136, 174, 203, 239, 263, 813, 822, 1159
DpnII GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
DraI TTTAAA 1 cut(s) 877
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
Eam1104I CTCTTC 3 cut(s) 186, 654, 664
EarI CTCTTC 3 cut(s) 186, 654, 664
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 656
Eco47I GGWCC 2 cut(s) 629, 1199
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 701
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 701
Esp3I CGTCTC 1 cut(s) 1077
FaeI CATG 4 cut(s) 200, 238, 812, 1148
FaiI YATR 9 cut(s) 198, 218, 236, 339, 759, 810, 1022, 1077, 1146
FaqI GGGAC 3 cut(s) 642, 816, 1212
FatI CATG 4 cut(s) 196, 234, 808, 1144
FauI CCCGC 2 cut(s) 92, 347
FbaI TGATCA 2 cut(s) 201, 820
FokI GGATG 3 cut(s) 502, 611, 728
FriOI GRGCYC 1 cut(s) 181
FspBI CTAG 2 cut(s) 486, 795
FspI TGCGCA 2 cut(s) 440, 732
GlaI GCGC 4 cut(s) 110, 385, 440, 732
GsuI CTGGAG 2 cut(s) 140, 1173
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 2 cut(s) 611, 1001
HapII CCGG 5 cut(s) 365, 666, 816, 834, 1104
HgaI GACGC 1 cut(s) 565
HhaI GCGC 4 cut(s) 111, 386, 441, 733
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 4 cut(s) 200, 238, 812, 1148
Hin6I GCGC 4 cut(s) 109, 384, 439, 731
HinP1I GCGC 4 cut(s) 109, 384, 439, 731
HincII GTYRAC 3 cut(s) 457, 483, 930
HindII GTYRAC 3 cut(s) 457, 483, 930
HpaII CCGG 5 cut(s) 365, 666, 816, 834, 1104
HphI GGTGA 4 cut(s) 158, 830, 926, 979
Hpy166II GTNNAC 4 cut(s) 457, 483, 842, 930
Hpy188III TCNNGA 9 cut(s) 100, 235, 259, 302, 348, 365, 374, 463, 809
Hpy8I GTNNAC 4 cut(s) 457, 483, 842, 930
Hpy99I CGWCG 3 cut(s) 143, 146, 1224
HpyAV CCTTC 8 cut(s) 24, 307, 508, 582, 1009, 1098, 1105, 1189
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 2 cut(s) 141, 225
HpyF3I CTNAG 4 cut(s) 126, 585, 982, 1211
HpySE526I ACGT 2 cut(s) 141, 225
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 4 cut(s) 200, 238, 812, 1148
HspAI GCGC 4 cut(s) 109, 384, 439, 731
Kpn2I TCCGGA 1 cut(s) 364
Ksp22I TGATCA 2 cut(s) 201, 820
Kzo9I GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 4 cut(s) 156, 193, 208, 367
Lsp1109I GCAGC 7 cut(s) 520, 715, 749, 757, 764, 773, 791
LweI GCATC 8 cut(s) 7, 341, 459, 480, 589, 715, 757, 976
MaeI CTAG 2 cut(s) 486, 795
MaeII ACGT 2 cut(s) 141, 225
MaeIII GTNAC 2 cut(s) 35, 164
MalI GATC 8 cut(s) 136, 174, 203, 239, 263, 813, 822, 1159
MbiI CCGCTC 1 cut(s) 422
MboI GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
MboII GAAGA 7 cut(s) 18, 173, 176, 575, 671, 681, 927
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 1 cut(s) 1157
MhlI GDGCHC 3 cut(s) 125, 181, 522
MluCI AATT 4 cut(s) 413, 445, 528, 1083
MlyI GAGTC 7 cut(s) 43, 462, 755, 783, 878, 1127, 1210
MmeI TCCRAC 2 cut(s) 162, 201
MroI TCCGGA 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 4 cut(s) 696, 876, 941, 1184
MslI CAYNNNNRTG 2 cut(s) 434, 467
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 5 cut(s) 365, 666, 816, 834, 1104
MspR9I CCNGG 3 cut(s) 113, 816, 834
MunI CAATTG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 4 cut(s) 189, 384, 386, 1180
NciI CCSGG 2 cut(s) 816, 834
NdeII GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
NlaIII CATG 4 cut(s) 200, 238, 812, 1148
NlaIV GGNNCC 2 cut(s) 630, 1200
NmuCI GTSAC 2 cut(s) 35, 164
NsbI TGCGCA 2 cut(s) 440, 732
NspI RCATGY 2 cut(s) 200, 1148
PagI TCATGA 2 cut(s) 234, 808
PciI ACATGT 1 cut(s) 1144
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 1225
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 7 cut(s) 379, 803, 884, 890, 917, 950, 970
PflFI GACNNNGTC 1 cut(s) 143
PfoI TCCNGGA 1 cut(s) 832
PleI GAGTC 7 cut(s) 42, 461, 754, 783, 877, 1126, 1210
PpsI GAGTC 7 cut(s) 42, 461, 754, 783, 877, 1126, 1210
PscI ACATGT 1 cut(s) 1144
Psp124BI GAGCTC 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 630, 1200
PspPI GGNCC 2 cut(s) 629, 1199
PstI CTGCAG 4 cut(s) 538, 658, 754, 1141
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 1 cut(s) 1157
PsyI GACNNNGTC 1 cut(s) 143
RsaI GTAC 1 cut(s) 1143
RsaNI GTAC 1 cut(s) 1142
RseI CAYNNNNRTG 2 cut(s) 434, 467
SacI GAGCTC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 4 cut(s) 696, 876, 941, 1184
Sau3AI GATC 8 cut(s) 134, 172, 201, 237, 261, 811, 820, 1157
Sau96I GGNCC 2 cut(s) 629, 1199
SchI GAGTC 7 cut(s) 43, 462, 755, 783, 878, 1127, 1210
ScrFI CCNGG 3 cut(s) 113, 816, 834
SduI GDGCHC 3 cut(s) 125, 181, 522
SfaNI GCATC 8 cut(s) 7, 341, 459, 480, 589, 715, 757, 976
SfcI CTRYAG 4 cut(s) 534, 654, 750, 1137
SinI GGWCC 2 cut(s) 629, 1199
SmiMI CAYNNNNRTG 2 cut(s) 434, 467
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 4 cut(s) 413, 445, 528, 1083
SspMI CTAG 2 cut(s) 486, 795
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 3 cut(s) 111, 814, 832
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 2 cut(s) 144, 228
TasI AATT 4 cut(s) 413, 445, 528, 1083
TatI WGTACW 1 cut(s) 1141
TauI GCSGC 3 cut(s) 344, 422, 992
TfiI GAWTC 7 cut(s) 379, 803, 884, 890, 917, 950, 970
Tru1I TTAA 4 cut(s) 696, 876, 941, 1184
Tru9I TTAA 4 cut(s) 696, 876, 941, 1184
TscAI CASTG 3 cut(s) 400, 589, 789
TseFI GTSAC 2 cut(s) 35, 164
TseI GCWGC 7 cut(s) 533, 728, 737, 752, 761, 770, 779
Tsp45I GTSAC 2 cut(s) 35, 164
TspDTI ATGAA 3 cut(s) 263, 321, 527
TspGWI ACGGA 1 cut(s) 565
TspRI CASTG 3 cut(s) 400, 589, 789
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 629, 1199
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 413
XceI RCATGY 2 cut(s) 200, 1148
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 2 cut(s) 486, 795
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.