Rh4CG364200

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012396
Physical Location & Seq
Forward (+)
62048921 .. 62050400
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG364200.1

Sequence Viewer

Length: 1218 bp
ATGGGAAGAAAGCTGGACGCTTTGCTTGGAAGGAGCCAGAAGACCGCAAAGTTGAAACCACTCCTCAGTCTTGCTCTCTCCCGCGTCGCCGTCCTCAAAACCCAGCGTCAGGCCAAGTGTAGCCAGTCCCGCTCCGATGTGCTCCAGCTCCTCCAGCTCGGTAACCATGACCGAGCTCTTCTTCGAGTTGAGCATGTGATCAAGGAGCAGAACATGCTGGATGTGTATGTCATGATCGAGCGTTATTGCAACCTCTTGATCGAAAGAGTTCACCTCATTGAACAAGAAAGGGAGTGTCCAGAGGAATTGAGGGAGGCAGCATCGAGTATGCTTTATGCTGCTTCGAGATGTGGAGATTTCCCGGAGCTTCAAGAGATCCGTAACATTCTCAGCGCTCGTTTTGGCAAGGAATTCACTGCTCGTTCCATTGATCTGCGCAACAATTGCGGAGTCAACCTTACCATGATCCAGAAACTGTCGACTAGGATGCCAACTAGGGAGAGCAGAATGAAAGTGCTTAAAGAAATTGCTTTCGATAATTCCATTGTTCTGAATCTAGAAGACACTTCTGGTTCCGTTGAGGATAAGAAGGTCCAGACACCACCACACCCACTCGCCAGTTCAAGTGGCACGGCTGGATCAGCAGAAGTTTTGCAGGCTTTTAAAGAAGAGACGGAAAAAGATGATAAGTATTCTCAGAAGAAATACAAGGATGTGGCTCATGCAGCTCAGGCTGCTTTTGAGTCTGCAGCTTATGCAGCAGCAGCTGCAAGAGCAGCCGTGGAACTGTCCAGATCCGAAACTCATGATTCTGATGATCAAAACAGTCCTGGTCCTAAACGAGGCAAGTTATCTAGCAGATACGAGTCCTCAAAAACTGAATTTGGATCACAGAATGAGCACATCACTGTTCAGAATCAAGCTGAAGAATTGAAGAGGTCAGCATCTTCTTCAAGTTCAGATTCAGGAGGTGACACACTGGAGGTGACTCCTCCAATGTCCTCCGATGCCATAGGCCAGCCCAGTCATTCAGGCAAGGTCACAGTTTTTGATGATAGTGACAATGAAGAAAGTACCATGGCCCTATCTCCAGAGAAAGTTCCTTCAAGGGTTCAATCTGGCATGAAGGTCGAGCCAGAGGGATCATCTAGAAGGCTAACATTAAACCTGGAGAAAGGACCATTCTCTCTGAGAAATAGATGGGTCCGTGGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

405

Amino Acids

44.84

Weight (kDa)

7.13

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 19 - 182 1.4e-54 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 479
AccII CGCG 1 cut(s) 84
AciI CCGC 4 cut(s) 45, 82, 130, 447
AclWI GGATC 6 cut(s) 370, 460, 646, 789, 895, 1150
AcoI YGGCCR 1 cut(s) 1210
AcsI RAATTY 2 cut(s) 410, 881
AcuI CTGAAG 1 cut(s) 945
AfaI GTAC 1 cut(s) 1075
AfeI AGCGCT 1 cut(s) 394
AfiI CCNNNNNNNGG 2 cut(s) 109, 842
AgsI TTSAA 8 cut(s) 55, 281, 371, 624, 934, 954, 1107, 1115
AjnI CCWGG 2 cut(s) 829, 1167
AluBI AGCT 9 cut(s) 13, 148, 157, 176, 367, 728, 752, 767, 923
AluI AGCT 9 cut(s) 13, 148, 157, 176, 367, 728, 752, 767, 923
Alw21I GWGCWC 3 cut(s) 144, 178, 903
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 6 cut(s) 370, 460, 646, 789, 895, 1150
AlwNI CAGNNNCTG 2 cut(s) 475, 767
Aor51HI AGCGCT 1 cut(s) 394
AoxI GGCC 4 cut(s) 111, 1015, 1080, 1210
ApoI RAATTY 2 cut(s) 410, 881
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 395, 438
AspS9I GGNCC 5 cut(s) 592, 833, 1081, 1178, 1204
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 3 cut(s) 263, 983, 997
AvaII GGWCC 4 cut(s) 592, 833, 1178, 1204
BalI TGGCCA 1 cut(s) 1212
BanII GRGCYC 1 cut(s) 178
BbsI GAAGAC 2 cut(s) 47, 567
Bbv12I GWGCWC 3 cut(s) 144, 178, 903
BccI CCATC 1 cut(s) 1194
BceAI ACGGC 3 cut(s) 74, 648, 764
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 1111, 1145
BciT130I CCWGG 2 cut(s) 831, 1169
BclI TGATCA 2 cut(s) 198, 817
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 5 cut(s) 483, 495, 557, 855, 1149
BfmI CTRYAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 396
Bme1390I CCNGG 3 cut(s) 362, 831, 1169
Bme18I GGWCC 4 cut(s) 592, 833, 1178, 1204
BmgT120I GGNCC 5 cut(s) 592, 833, 1081, 1178, 1204
BmiI GGNNCC 3 cut(s) 35, 574, 1205
BmrFI CCNGG 3 cut(s) 362, 831, 1169
BmrI ACTGGG 1 cut(s) 1017
BmsI GCATC 4 cut(s) 329, 477, 953, 997
BmuI ACTGGG 1 cut(s) 1017
BpiI GAAGAC 2 cut(s) 47, 567
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 5 cut(s) 128, 137, 1001, 1074, 1190
Bpu10I CCTNAGC 1 cut(s) 729
BpuMI CCSGG 1 cut(s) 362
BsaJI CCNNGG 3 cut(s) 780, 1077, 1207
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 842
Bse1I ACTGG 4 cut(s) 124, 618, 984, 1023
BseBI CCWGG 2 cut(s) 831, 1169
BseDI CCNNGG 3 cut(s) 780, 1077, 1207
BseGI GGATG 3 cut(s) 226, 492, 718
BseLI CCNNNNNNNGG 2 cut(s) 109, 842
BseMII CTCAG 5 cut(s) 79, 403, 710, 743, 1181
BseNI ACTGG 4 cut(s) 124, 618, 984, 1023
BseRI GAGGAG 3 cut(s) 53, 140, 981
BseYI CCCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 113, 1017, 1082, 1212
BsiHKAI GWGCWC 3 cut(s) 144, 178, 903
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 109, 842
BsmAI GTCTC 1 cut(s) 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 4 cut(s) 113, 1017, 1082, 1212
Bsp1286I GDGCHC 3 cut(s) 144, 178, 903
Bsp19I CCATGG 1 cut(s) 1077
BspACI CCGC 4 cut(s) 45, 82, 130, 447
BspANI GGCC 4 cut(s) 113, 1017, 1082, 1212
BspCNI CTCAG 5 cut(s) 78, 402, 709, 742, 1182
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 2 cut(s) 231, 805
BspLI GGNNCC 3 cut(s) 35, 574, 1205
BspMAI CTGCAG 1 cut(s) 751
BspPI GGATC 6 cut(s) 370, 460, 646, 789, 895, 1150
BspQI GCTCTTC 1 cut(s) 183
BsrBI CCGCTC 1 cut(s) 132
BsrI ACTGG 4 cut(s) 124, 618, 984, 1023
BssECI CCNNGG 3 cut(s) 780, 1077, 1207
BssT1I CCWWGG 1 cut(s) 1077
Bst2UI CCWGG 2 cut(s) 831, 1169
Bst4CI ACNGT 5 cut(s) 477, 789, 827, 910, 1045
Bst6I CTCTTC 3 cut(s) 183, 663, 929
BstAPI GCANNNNNTGC 4 cut(s) 214, 444, 755, 767
BstC8I GCNNGC 2 cut(s) 657, 1019
BstDEI CTNAG 5 cut(s) 65, 389, 696, 729, 1190
BstDSI CCRYGG 3 cut(s) 780, 1077, 1207
BstEII GGTNACC 1 cut(s) 161
BstENI CCTNNNNNAGG 1 cut(s) 840
BstF5I GGATG 3 cut(s) 226, 492, 718
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 2 cut(s) 395, 438
BstMAI GTCTC 1 cut(s) 665
BstNI CCWGG 2 cut(s) 831, 1169
BstNSI RCATGY 2 cut(s) 197, 217
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 360, 829, 1167
BstSFI CTRYAG 1 cut(s) 747
BstUI CGCG 1 cut(s) 84
BstV2I GAAGAC 2 cut(s) 47, 567
BstX2I RGATCY 2 cut(s) 375, 794
BstYI RGATCY 2 cut(s) 375, 794
BsuRI GGCC 4 cut(s) 113, 1017, 1082, 1212
BtgI CCRYGG 3 cut(s) 780, 1077, 1207
BtsCI GGATG 3 cut(s) 226, 492, 718
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 3 cut(s) 414, 906, 977
Cac8I GCNNGC 2 cut(s) 657, 1019
CaiI CAGNNNCTG 2 cut(s) 475, 767
CciI TCATGA 2 cut(s) 231, 805
CfoI GCGC 2 cut(s) 395, 438
Cfr13I GGNCC 5 cut(s) 592, 833, 1081, 1178, 1204
CseI GACGC 3 cut(s) 26, 73, 95
Csp6I GTAC 1 cut(s) 1074
CviAII CATG 9 cut(s) 167, 194, 214, 232, 463, 722, 806, 1078, 1123
CviQI GTAC 1 cut(s) 1074
DdeI CTNAG 5 cut(s) 65, 389, 696, 729, 1190
DraI TTTAAA 1 cut(s) 664
EaeI YGGCCR 1 cut(s) 1210
Eam1104I CTCTTC 3 cut(s) 183, 663, 929
EarI CTCTTC 3 cut(s) 183, 663, 929
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 1077
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 4 cut(s) 592, 833, 1178, 1204
Eco47III AGCGCT 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 945
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 840
EcoO65I GGTNACC 1 cut(s) 161
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 829, 1167
EcoT14I CCWWGG 1 cut(s) 1077
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 1077
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 9 cut(s) 170, 197, 217, 235, 466, 725, 809, 1081, 1126
FaqI GGGAC 1 cut(s) 112
FatI CATG 9 cut(s) 166, 193, 213, 231, 462, 721, 805, 1077, 1122
FauI CCCGC 2 cut(s) 89, 137
FbaI TGATCA 2 cut(s) 198, 817
FblI GTMKAC 1 cut(s) 479
FokI GGATG 3 cut(s) 233, 499, 725
FriOI GRGCYC 1 cut(s) 178
FspBI CTAG 5 cut(s) 483, 495, 557, 855, 1149
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 2 cut(s) 394, 437
GsaI CCCAGC 1 cut(s) 106
GsuI CTGGAG 5 cut(s) 128, 137, 1001, 1074, 1190
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 4 cut(s) 113, 1017, 1082, 1212
HapII CCGG 1 cut(s) 362
HgaI GACGC 3 cut(s) 26, 73, 95
HhaI GCGC 2 cut(s) 395, 438
Hin1II CATG 9 cut(s) 170, 197, 217, 235, 466, 725, 809, 1081, 1126
Hin6I GCGC 2 cut(s) 393, 436
HinP1I GCGC 2 cut(s) 393, 436
HincII GTYRAC 2 cut(s) 454, 480
HindII GTYRAC 2 cut(s) 454, 480
HinfI GANTC 8 cut(s) 450, 553, 743, 809, 866, 916, 962, 988
HpaII CCGG 1 cut(s) 362
HphI GGTGA 3 cut(s) 263, 983, 997
Hpy166II GTNNAC 3 cut(s) 271, 454, 480
Hpy188I TCNGA 9 cut(s) 136, 552, 699, 799, 814, 915, 961, 1006, 1191
Hpy8I GTNNAC 3 cut(s) 271, 454, 480
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 5 cut(s) 24, 583, 1113, 1120, 1146
HpyCH4III ACNGT 5 cut(s) 477, 789, 827, 910, 1045
HpyCH4V TGCA 6 cut(s) 249, 655, 725, 749, 758, 770
HpyF3I CTNAG 5 cut(s) 65, 389, 696, 729, 1190
Hsp92II CATG 9 cut(s) 170, 197, 217, 235, 466, 725, 809, 1081, 1126
HspAI GCGC 2 cut(s) 393, 436
Ksp22I TGATCA 2 cut(s) 198, 817
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 6 cut(s) 33, 137, 147, 153, 205, 364
LweI GCATC 4 cut(s) 329, 477, 953, 997
MaeI CTAG 5 cut(s) 483, 495, 557, 855, 1149
MaeIII GTNAC 6 cut(s) 161, 380, 971, 985, 1039, 1058
MbiI CCGCTC 1 cut(s) 132
MfeI CAATTG 1 cut(s) 442
MflI RGATCY 2 cut(s) 375, 794
MhlI GDGCHC 3 cut(s) 144, 178, 903
MlsI TGGCCA 1 cut(s) 1212
MluCI AATT 7 cut(s) 305, 410, 442, 525, 538, 881, 929
MluNI TGGCCA 1 cut(s) 1212
MlyI GAGTC 4 cut(s) 459, 752, 875, 982
Mox20I TGGCCA 1 cut(s) 1212
MroXI GAANNNNTTC 1 cut(s) 267
MscI TGGCCA 1 cut(s) 1212
MseI TTAA 3 cut(s) 519, 663, 1163
Msp20I TGGCCA 1 cut(s) 1212
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 3 cut(s) 362, 831, 1169
MunI CAATTG 1 cut(s) 442
MvaI CCWGG 2 cut(s) 831, 1169
MvnI CGCG 1 cut(s) 84
NciI CCSGG 1 cut(s) 362
NcoI CCATGG 1 cut(s) 1077
NlaIII CATG 9 cut(s) 170, 197, 217, 235, 466, 725, 809, 1081, 1126
NlaIV GGNNCC 3 cut(s) 35, 574, 1205
NmuCI GTSAC 4 cut(s) 971, 985, 1039, 1058
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 2 cut(s) 197, 217
PagI TCATGA 2 cut(s) 231, 805
PciSI GCTCTTC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 4 cut(s) 553, 809, 916, 962
PfoI TCCNGGA 1 cut(s) 360
PleI GAGTC 4 cut(s) 458, 751, 874, 982
PpsI GAGTC 4 cut(s) 458, 751, 874, 982
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 829, 1167
PspEI GGTNACC 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 2 cut(s) 829, 1167
PspN4I GGNNCC 3 cut(s) 35, 574, 1205
PspPI GGNCC 5 cut(s) 592, 833, 1081, 1178, 1204
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 2 cut(s) 475, 767
PsuI RGATCY 2 cut(s) 375, 794
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 1 cut(s) 1075
RsaNI GTAC 1 cut(s) 1074
SacI GAGCTC 1 cut(s) 178
SalI GTCGAC 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 3 cut(s) 519, 663, 1163
Sau96I GGNCC 5 cut(s) 592, 833, 1081, 1178, 1204
SchI GAGTC 4 cut(s) 459, 752, 875, 982
ScrFI CCNGG 3 cut(s) 362, 831, 1169
SduI GDGCHC 3 cut(s) 144, 178, 903
SfaNI GCATC 4 cut(s) 329, 477, 953, 997
SfcI CTRYAG 1 cut(s) 747
SinI GGWCC 4 cut(s) 592, 833, 1178, 1204
Sse9I AATT 7 cut(s) 305, 410, 442, 525, 538, 881, 929
SsiI CCGC 4 cut(s) 45, 82, 130, 447
SspMI CTAG 5 cut(s) 483, 495, 557, 855, 1149
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 3 cut(s) 360, 829, 1167
StyI CCWWGG 1 cut(s) 1077
TaaI ACNGT 5 cut(s) 477, 789, 827, 910, 1045
TaqI TCGA 8 cut(s) 184, 237, 261, 323, 344, 479, 534, 1131
TaqII GACCGA 1 cut(s) 186
TasI AATT 7 cut(s) 305, 410, 442, 525, 538, 881, 929
TfiI GAWTC 4 cut(s) 553, 809, 916, 962
Tru1I TTAA 3 cut(s) 519, 663, 1163
Tru9I TTAA 3 cut(s) 519, 663, 1163
TscAI CASTG 3 cut(s) 421, 913, 984
TseFI GTSAC 4 cut(s) 971, 985, 1039, 1058
Tsp45I GTSAC 4 cut(s) 971, 985, 1039, 1058
TspDTI ATGAA 3 cut(s) 524, 1080, 1139
TspGWI ACGGA 4 cut(s) 368, 565, 689, 1196
TspRI CASTG 3 cut(s) 421, 913, 984
VpaK11BI GGWCC 4 cut(s) 592, 833, 1178, 1204
XagI CCTNNNNNAGG 1 cut(s) 840
XapI RAATTY 2 cut(s) 410, 881
XbaI TCTAGA 2 cut(s) 556, 1148
XceI RCATGY 2 cut(s) 197, 217
XmiI GTMKAC 1 cut(s) 479
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 5 cut(s) 483, 495, 557, 855, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.