MD13G1043900.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012396
Physical Location & Seq
Forward (+)
3065300 .. 3067475
2176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1043900.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGGAAGAAAGCTCGATGCTTTGCTTGGAAGGAGTCACAAGAACACCGCCAAGTTCAAACCCACTTTGAACCTCGCTCTCTCCCGCCTTGCCGTTCTTAAGAAACAGCGCCAGGCCAAGTGCTCTCAGGCTCGATCCGATGTCGTCCAGCTCCTCCAGCAGGGTCACCAAGATCGCGCTCTTCTTCGCGTGGGGCATGTGATTAACGAGCAGAATATGTTGGATGTGTTTGTCATGATAGAGGGTTATTGCAACCTAGTGATCGAAAGGGTTCATCTCATTGAACAAGAAAGGGAATGCCCTGATGAACTGAGGGAGGCAACATCGAGTTTGCTTTATGCGGCTTCAAGATGTGGGGATTTTCCGGAGCTTCAAGAGATTCGTGCGGTTCTCACTGCTCGTTTTGGCAAGGAATTTGCTTCTCGTGCCATCGAAGTGCGCAACAATTGTGGAGTCAACCTCACGATGATGCAAAAACTGTCAACTAGGATGCCAGTTTTGGAGGTCAGAATGAAGGTGCTCAAAGAAATTGCTGCAGAGAATAACATTGTTCTGAAGCTGGAAGAAACGTCTTCTGTTTACACCGAGGATAAATCGGATGTGAACAAGGCCGGTTCAAGTGGCACGGGACCAGCAGATAATGTGCAGGCTTCTGCAGAAGAGACGGGAAATGATGATAGGTTCTCTGGTTCGATTAAGCCAAGGAAATACAAGGATGTAGCTGATGCAGCTCAAGCAGCTTTCGAGTCTGCAGCTTACGCAGCAGATGCTGCTAGAGCAGCAGTGCAACTCTCTAGGTCTGAATCTCAAGATCCTGATGATCAAAATAGTCCCGGAAGTAAACGAGGAAAGTTATCTGACAGATATGAATCCTTTAAAAGTCTATCCGAATCGCAGCATGAGCGAAGTCGAGGTGAATTAAAGAGGTCAACATCTTCTTCAAGTTTAGATTCAGATGGAGATGAGGTGATTCCAATGTCCTCAGATGCCGTAGGGCAGGCCGATCTTTTAGGGAAGGATATAGTTTTTGATGAGAGTGAAAGCGACAACGAACCAAATACAACGCCCTCGTCTCATAACCAAATTCCTTCGAGGTTTCGATCTGGCTTGAAGGCGGAGTCAAGGCTTGAATATCCTGCAGCACATGTCACTGGAGGATCTGGAAGGCAAAGCCCGCCACGACTAAACATAGAGAAGGGACCATTCTCTCTGAGGACTAGACGGGTGCGTGGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

413

Amino Acids

45.39

Weight (kDa)

7.07

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 20 - 183 1e-55 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 440
AccII CGCG 2 cut(s) 177, 189
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 6 cut(s) 48, 85, 341, 386, 1115, 1175
AclWI GGATC 3 cut(s) 129, 806, 1165
AcsI RAATTY 2 cut(s) 413, 1083
AcuI CTGAAG 1 cut(s) 575
AfiI CCNNNNNNNGG 1 cut(s) 160
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 1 cut(s) 1144
AgsI TTSAA 9 cut(s) 58, 70, 284, 348, 374, 618, 942, 1111, 1130
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 8 cut(s) 13, 151, 370, 559, 722, 731, 740, 755
AluI AGCT 8 cut(s) 13, 151, 370, 559, 722, 731, 740, 755
Alw21I GWGCWC 2 cut(s) 125, 522
Alw26I GTCTC 2 cut(s) 656, 1077
AlwI GGATC 3 cut(s) 129, 806, 1165
AlwNI CAGNNNCTG 1 cut(s) 770
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 3 cut(s) 114, 609, 999
ApeKI GCWGC 9 cut(s) 533, 728, 737, 752, 761, 770, 779, 895, 1139
ApoI RAATTY 2 cut(s) 413, 1083
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 3 cut(s) 111, 179, 441
AspS9I GGNCC 2 cut(s) 629, 1199
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 3 cut(s) 158, 926, 979
AvaII GGWCC 2 cut(s) 629, 1199
BauI CACGAG 1 cut(s) 423
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 2 cut(s) 125, 522
BbvI GCAGC 9 cut(s) 520, 740, 749, 757, 764, 773, 791, 907, 1151
BccI CCATC 2 cut(s) 437, 950
BceAI ACGGC 2 cut(s) 77, 974
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 2 cut(s) 656, 1077
BfaI CTAG 5 cut(s) 257, 486, 774, 795, 1218
BfmI CTRYAG 4 cut(s) 534, 654, 750, 1137
BfoI RGCGCY 1 cut(s) 112
BfrI CTTAAG 1 cut(s) 98
Bme1390I CCNGG 2 cut(s) 113, 834
Bme18I GGWCC 2 cut(s) 629, 1199
BmgT120I GGNCC 2 cut(s) 629, 1199
BmiI GGNNCC 2 cut(s) 630, 1200
BmrFI CCNGG 2 cut(s) 113, 834
BmsI GCATC 6 cut(s) 7, 459, 480, 715, 757, 976
BpiI GAAGAC 1 cut(s) 564
BplI GAGNNNNNCTC 2 cut(s) 444, 476
BpmI CTGGAG 2 cut(s) 140, 1173
BpuEI CTTGAG 2 cut(s) 717, 792
BpuMI CCSGG 1 cut(s) 834
BsaBI GATNNNNATC 1 cut(s) 868
BsaJI CCNNGG 2 cut(s) 585, 701
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 951, 981
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 2 cut(s) 494, 1156
Bse8I GATNNNNATC 1 cut(s) 868
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 2 cut(s) 585, 701
BseGI GGATG 4 cut(s) 229, 495, 604, 721
BseJI GATNNNNATC 1 cut(s) 868
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 4 cut(s) 140, 302, 996, 1202
BseNI ACTGG 2 cut(s) 494, 1156
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 9 cut(s) 520, 740, 749, 757, 764, 773, 791, 907, 1151
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 2 cut(s) 177, 189
BshFI GGCC 3 cut(s) 116, 611, 1001
BsiHKAI GWGCWC 2 cut(s) 125, 522
BsiSI CCGG 3 cut(s) 365, 612, 834
BslFI GGGAC 3 cut(s) 642, 816, 1212
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 656, 1077
BsmBI CGTCTC 2 cut(s) 656, 1077
BsmFI GGGAC 3 cut(s) 642, 816, 1212
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 3 cut(s) 116, 611, 1001
Bsp1286I GDGCHC 2 cut(s) 125, 522
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
BspACI CCGC 6 cut(s) 48, 85, 341, 386, 1115, 1175
BspANI GGCC 3 cut(s) 116, 611, 1001
BspCNI CTCAG 4 cut(s) 139, 303, 995, 1203
BspEI TCCGGA 1 cut(s) 364
BspFNI CGCG 2 cut(s) 177, 189
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 630, 1200
BspMAI CTGCAG 4 cut(s) 538, 658, 754, 1141
BspPI GGATC 3 cut(s) 129, 806, 1165
BspQI GCTCTTC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 2 cut(s) 494, 1156
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 2 cut(s) 585, 701
BssMI GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 701
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 2 cut(s) 186, 654
BstAFI CTTAAG 1 cut(s) 98
BstAPI GCANNNNNTGC 2 cut(s) 767, 770
BstC8I GCNNGC 3 cut(s) 648, 999, 1175
BstDEI CTNAG 4 cut(s) 126, 311, 982, 1211
BstEII GGTNACC 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 229, 495, 604, 721
BstFNI CGCG 2 cut(s) 177, 189
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 3 cut(s) 111, 179, 441
BstKTI GATC 8 cut(s) 137, 175, 264, 814, 823, 1006, 1103, 1160
BstMAI GTCTC 2 cut(s) 656, 1077
BstMBI GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 200, 1148
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 111, 832
BstSFI CTRYAG 4 cut(s) 534, 654, 750, 1137
BstUI CGCG 2 cut(s) 177, 189
BstV1I GCAGC 9 cut(s) 520, 740, 749, 757, 764, 773, 791, 907, 1151
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 2 cut(s) 811, 1157
BstYI RGATCY 2 cut(s) 811, 1157
BsuRI GGCC 3 cut(s) 116, 611, 1001
BtsCI GGATG 4 cut(s) 229, 495, 604, 721
BtsI GCAGTG 2 cut(s) 393, 789
BtsIMutI CAGTG 3 cut(s) 393, 789, 1149
Cac8I GCNNGC 3 cut(s) 648, 999, 1175
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 1 cut(s) 234
CfoI GCGC 3 cut(s) 111, 179, 441
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 2 cut(s) 629, 1199
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 4 cut(s) 197, 235, 899, 1145
DdeI CTNAG 4 cut(s) 126, 311, 982, 1211
DpnI GATC 8 cut(s) 136, 174, 263, 813, 822, 1005, 1102, 1159
DpnII GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
DraI TTTAAA 1 cut(s) 877
Eam1104I CTCTTC 2 cut(s) 186, 654
EarI CTCTTC 2 cut(s) 186, 654
EciI GGCGGA 1 cut(s) 1130
Eco130I CCWWGG 1 cut(s) 701
Eco47I GGWCC 2 cut(s) 629, 1199
Eco57I CTGAAG 1 cut(s) 575
Eco91I GGTNACC 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 701
ErhI CCWWGG 1 cut(s) 701
Esp3I CGTCTC 2 cut(s) 656, 1077
FaeI CATG 4 cut(s) 200, 238, 902, 1148
FaqI GGGAC 3 cut(s) 642, 816, 1212
FatI CATG 4 cut(s) 196, 234, 898, 1144
FauI CCCGC 2 cut(s) 92, 1182
FbaI TGATCA 1 cut(s) 820
FokI GGATG 4 cut(s) 236, 502, 611, 728
FspBI CTAG 5 cut(s) 257, 486, 774, 795, 1218
FspI TGCGCA 1 cut(s) 440
GlaI GCGC 3 cut(s) 110, 178, 440
GsuI CTGGAG 2 cut(s) 140, 1173
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 3 cut(s) 116, 611, 1001
HapII CCGG 3 cut(s) 365, 612, 834
HhaI GCGC 3 cut(s) 111, 179, 441
Hin1II CATG 4 cut(s) 200, 238, 902, 1148
Hin6I GCGC 3 cut(s) 109, 177, 439
HinP1I GCGC 3 cut(s) 109, 177, 439
HincII GTYRAC 3 cut(s) 457, 483, 930
HindII GTYRAC 3 cut(s) 457, 483, 930
HpaII CCGG 3 cut(s) 365, 612, 834
HphI GGTGA 3 cut(s) 158, 926, 979
Hpy166II GTNNAC 6 cut(s) 457, 483, 580, 604, 842, 930
Hpy188III TCNNGA 8 cut(s) 235, 348, 365, 374, 463, 809, 815, 1161
Hpy8I GTNNAC 6 cut(s) 457, 483, 580, 604, 842, 930
HpyAV CCTTC 7 cut(s) 24, 508, 1009, 1098, 1105, 1158, 1189
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 569
HpyCH4V TGCA 9 cut(s) 252, 472, 536, 646, 656, 728, 752, 787, 1139
HpyF3I CTNAG 4 cut(s) 126, 311, 982, 1211
HpySE526I ACGT 1 cut(s) 569
Hsp92II CATG 4 cut(s) 200, 238, 902, 1148
HspAI GCGC 3 cut(s) 109, 177, 439
Kpn2I TCCGGA 1 cut(s) 364
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 2 cut(s) 156, 367
Lsp1109I GCAGC 9 cut(s) 520, 740, 749, 757, 764, 773, 791, 907, 1151
LweI GCATC 6 cut(s) 7, 459, 480, 715, 757, 976
MaeI CTAG 5 cut(s) 257, 486, 774, 795, 1218
MaeII ACGT 1 cut(s) 569
MaeIII GTNAC 3 cut(s) 35, 164, 1147
MalI GATC 8 cut(s) 136, 174, 263, 813, 822, 1005, 1102, 1159
MboI GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
MboII GAAGA 8 cut(s) 18, 173, 176, 564, 575, 671, 927, 930
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 2 cut(s) 811, 1157
MhlI GDGCHC 2 cut(s) 125, 522
MluCI AATT 5 cut(s) 413, 445, 528, 917, 1083
MlyI GAGTC 4 cut(s) 43, 462, 755, 1127
MmeI TCCRAC 1 cut(s) 201
MroI TCCGGA 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 5 cut(s) 99, 204, 696, 876, 920
MslI CAYNNNNRTG 2 cut(s) 434, 467
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 3 cut(s) 365, 612, 834
MspR9I CCNGG 2 cut(s) 113, 834
MunI CAATTG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 2 cut(s) 177, 189
NciI CCSGG 1 cut(s) 834
NdeII GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
NlaIII CATG 4 cut(s) 200, 238, 902, 1148
NlaIV GGNNCC 2 cut(s) 630, 1200
NmuCI GTSAC 3 cut(s) 35, 164, 1147
NsbI TGCGCA 1 cut(s) 440
NspI RCATGY 2 cut(s) 200, 1148
PagI TCATGA 1 cut(s) 234
PciI ACATGT 1 cut(s) 1144
PciSI GCTCTTC 1 cut(s) 186
PctI GAATGC 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 6 cut(s) 379, 803, 869, 890, 950, 970
PfoI TCCNGGA 1 cut(s) 832
PleI GAGTC 4 cut(s) 42, 461, 754, 1126
PpsI GAGTC 4 cut(s) 42, 461, 754, 1126
PscI ACATGT 1 cut(s) 1144
Psp6I CCWGG 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 630, 1200
PspPI GGNCC 2 cut(s) 629, 1199
PstI CTGCAG 4 cut(s) 538, 658, 754, 1141
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 2 cut(s) 811, 1157
RseI CAYNNNNRTG 2 cut(s) 434, 467
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 5 cut(s) 99, 204, 696, 876, 920
Sau3AI GATC 8 cut(s) 134, 172, 261, 811, 820, 1003, 1100, 1157
Sau96I GGNCC 2 cut(s) 629, 1199
SchI GAGTC 4 cut(s) 43, 462, 755, 1127
ScrFI CCNGG 2 cut(s) 113, 834
SduI GDGCHC 2 cut(s) 125, 522
SfaNI GCATC 6 cut(s) 7, 459, 480, 715, 757, 976
SfcI CTRYAG 4 cut(s) 534, 654, 750, 1137
SinI GGWCC 2 cut(s) 629, 1199
SmiMI CAYNNNNRTG 2 cut(s) 434, 467
SmlI CTYRAG 3 cut(s) 98, 732, 807
SmoI CTYRAG 3 cut(s) 98, 732, 807
Sse9I AATT 5 cut(s) 413, 445, 528, 917, 1083
SsiI CCGC 6 cut(s) 48, 85, 341, 386, 1115, 1175
SspMI CTAG 5 cut(s) 257, 486, 774, 795, 1218
StyD4I CCNGG 2 cut(s) 111, 832
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 572
TasI AATT 5 cut(s) 413, 445, 528, 917, 1083
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 6 cut(s) 379, 803, 869, 890, 950, 970
Tru1I TTAA 5 cut(s) 99, 204, 696, 876, 920
Tru9I TTAA 5 cut(s) 99, 204, 696, 876, 920
TscAI CASTG 3 cut(s) 400, 789, 1156
TseFI GTSAC 3 cut(s) 35, 164, 1147
TseI GCWGC 9 cut(s) 533, 728, 737, 752, 761, 770, 779, 895, 1139
Tsp45I GTSAC 3 cut(s) 35, 164, 1147
TspDTI ATGAA 4 cut(s) 263, 321, 527, 882
TspRI CASTG 3 cut(s) 400, 789, 1156
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 629, 1199
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 2 cut(s) 413, 1083
XceI RCATGY 2 cut(s) 200, 1148
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 5 cut(s) 257, 486, 774, 795, 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.