RLG00000006588

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012396
Physical Location & Seq
Reverse (-)
8737296 .. 8738780
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006588

Sequence Viewer

Length: 1215 bp
ATGGGAAGAAAGCTCGACGCTTTGCTTGGAAGGAGCCAGAAGACCGCAAAGTTGAAACCACTCCTCAGTCTTGCTCTCTCCCGCGTCACCGTCCTCAAAACCCAGCGTCAGGCCAGGTGTAGCCAGTCCCGCTCCGATGTGCTCCAGCTCCTCCAGCTCGGTAACCATGACCGAGCTCTTCTTCGAGTTGAGCATGTGATCAAGGAGCAGAACATGCTGGATGTGTATGTCATGATCGAGCGTTATTGCAACCTCTTGATCGAAAGAGTTCACCTCATTGAACAAGAAAGGGAGTGTCCAGAGGAATTGAGGGAGGCAGCATCGAGTATGCTTTATGCTGCTTCGAGATGTGGAGAATTCCCGGAGCTTCAAGAGATTCGTAACATTCTCAGTGCTCGTTTTGGCAAGGAATTCACCGCTCGTTCCATTGAACTGCGCAACAATTGCGGAGTCAACCTTACCATGATCCAAAAACTGTCGACTAGGATGCCAACTAGGGAGAGCAGAATGAAAGTGCTTAAAGAAATTGCTTTCGATAATTCCATTGTTCTGAACCTAGAAGACACTTCTGGTTCCGTTGAGGATAAGGTCCAGACACCACCACACCCACTTGCCAGTTCAAGTGGCACGGCTGGGTCAGCAGAAGTTTTGCAGGCTTTTAAAGAAGAGACGGAAAAAGATGATAAGTATTCTCAGAAGAAATACAAGGATGTGGCTCATGCAGCTCAGGCTGCTTTTGAGTCTGCAGCTTATGCAGCAGCAGCTGCAAGAGCAGCCGTGGAACTGTCCAGATCCGAAACTCATGATTCTGATGACCAAAACAGTCCTGGTCCTAAACGAGGCAAGTTATCTAGCAGATACGAGTCTTCAAAAACTGAATTTGGATCACAGAATGAGCACATCACTGTTCAGAATCAAGCTGAAGAATTGAAGAGGTCAGCATCTTCTTCAAGTGCAGAGTCAGGAGGTGACACACTGGAGGTGACTCCTCCAATGTCCTCCGATGCCATAGGCCAGCCCAGTCATTCAGGAAAGGTCACAGTTTTTGATGATAGTGACAATGAAGAAAGTACCATGGCCTTATCTCCAGAGAAAGTTCCTTCAAGGGTTCAATCTGGCACAAAGGTCGAGCCAGGGGGATCATCTAGAAGGCTAACATTAAACCTGGAGAAAGGACCATTCTCTCTGAGAAATAGATGGGTCCGTGGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

405

Amino Acids

44.69

Weight (kDa)

7.13

Isoelectric Point (pI)

62.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 19 - 182 1.4e-54 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 437
AccBSI CCGCTC 2 cut(s) 132, 419
AccI GTMKAC 1 cut(s) 479
AccII CGCG 1 cut(s) 84
AciI CCGC 5 cut(s) 45, 82, 130, 417, 447
AclWI GGATC 4 cut(s) 460, 786, 892, 1147
AcoI YGGCCR 1 cut(s) 1207
AcsI RAATTY 3 cut(s) 356, 410, 878
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 1 cut(s) 1072
AfiI CCNNNNNNNGG 2 cut(s) 109, 839
AjnI CCWGG 4 cut(s) 113, 826, 1132, 1164
AluBI AGCT 9 cut(s) 13, 148, 157, 176, 367, 725, 749, 764, 920
AluI AGCT 9 cut(s) 13, 148, 157, 176, 367, 725, 749, 764, 920
Alw21I GWGCWC 4 cut(s) 144, 178, 397, 900
Alw26I GTCTC 1 cut(s) 662
AlwI GGATC 4 cut(s) 460, 786, 892, 1147
AlwNI CAGNNNCTG 1 cut(s) 764
AoxI GGCC 4 cut(s) 111, 1012, 1077, 1207
ApoI RAATTY 3 cut(s) 356, 410, 878
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 4 cut(s) 589, 830, 1175, 1201
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 5 cut(s) 79, 263, 406, 980, 994
AvaII GGWCC 4 cut(s) 589, 830, 1175, 1201
BalI TGGCCA 1 cut(s) 1209
BanII GRGCYC 1 cut(s) 178
BbsI GAAGAC 3 cut(s) 47, 567, 858
Bbv12I GWGCWC 4 cut(s) 144, 178, 397, 900
BccI CCATC 1 cut(s) 1191
BceAI ACGGC 2 cut(s) 645, 761
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 1108, 1142
BciT130I CCWGG 4 cut(s) 115, 828, 1134, 1166
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 662
BfaI CTAG 5 cut(s) 483, 495, 557, 852, 1146
BfmI CTRYAG 1 cut(s) 744
Bme1390I CCNGG 5 cut(s) 115, 362, 828, 1134, 1166
Bme18I GGWCC 4 cut(s) 589, 830, 1175, 1201
BmgT120I GGNCC 4 cut(s) 589, 830, 1175, 1201
BmiI GGNNCC 3 cut(s) 35, 574, 1202
BmrFI CCNGG 5 cut(s) 115, 362, 828, 1134, 1166
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 4 cut(s) 329, 477, 950, 994
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 3 cut(s) 47, 567, 858
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 5 cut(s) 128, 137, 998, 1071, 1187
Bpu10I CCTNAGC 1 cut(s) 726
BpuMI CCSGG 1 cut(s) 362
BsaJI CCNNGG 4 cut(s) 777, 1074, 1133, 1204
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 839
Bse1I ACTGG 4 cut(s) 124, 615, 981, 1020
BseBI CCWGG 4 cut(s) 115, 828, 1134, 1166
BseDI CCNNGG 4 cut(s) 777, 1074, 1133, 1204
BseGI GGATG 3 cut(s) 226, 492, 715
BseLI CCNNNNNNNGG 2 cut(s) 109, 839
BseMII CTCAG 5 cut(s) 79, 403, 707, 740, 1178
BseNI ACTGG 4 cut(s) 124, 615, 981, 1020
BseRI GAGGAG 3 cut(s) 53, 140, 978
BseYI CCCAGC 2 cut(s) 102, 632
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 113, 1014, 1079, 1209
BsiHKAI GWGCWC 4 cut(s) 144, 178, 397, 900
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 109, 839
BsmAI GTCTC 1 cut(s) 662
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 4 cut(s) 113, 1014, 1079, 1209
Bsp1286I GDGCHC 4 cut(s) 144, 178, 397, 900
Bsp143I GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
Bsp19I CCATGG 1 cut(s) 1074
BspACI CCGC 5 cut(s) 45, 82, 130, 417, 447
BspANI GGCC 4 cut(s) 113, 1014, 1079, 1209
BspCNI CTCAG 5 cut(s) 78, 402, 706, 739, 1179
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 2 cut(s) 231, 802
BspLI GGNNCC 3 cut(s) 35, 574, 1202
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 4 cut(s) 460, 786, 892, 1147
BspQI GCTCTTC 1 cut(s) 183
BsrBI CCGCTC 2 cut(s) 132, 419
BsrI ACTGG 4 cut(s) 124, 615, 981, 1020
BssECI CCNNGG 4 cut(s) 777, 1074, 1133, 1204
BssMI GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
BssT1I CCWWGG 1 cut(s) 1074
Bst2UI CCWGG 4 cut(s) 115, 828, 1134, 1166
Bst4CI ACNGT 6 cut(s) 91, 477, 786, 824, 907, 1042
Bst6I CTCTTC 3 cut(s) 183, 660, 926
BstAPI GCANNNNNTGC 4 cut(s) 214, 444, 752, 764
BstC8I GCNNGC 2 cut(s) 654, 1016
BstDEI CTNAG 5 cut(s) 65, 389, 693, 726, 1187
BstDSI CCRYGG 3 cut(s) 777, 1074, 1204
BstEII GGTNACC 1 cut(s) 161
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 3 cut(s) 226, 492, 715
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 7 cut(s) 201, 237, 261, 468, 794, 887, 1142
BstMAI GTCTC 1 cut(s) 662
BstMBI GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
BstNI CCWGG 4 cut(s) 115, 828, 1134, 1166
BstNSI RCATGY 2 cut(s) 197, 217
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 5 cut(s) 113, 360, 826, 1132, 1164
BstSFI CTRYAG 1 cut(s) 744
BstUI CGCG 1 cut(s) 84
BstV2I GAAGAC 3 cut(s) 47, 567, 858
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
BsuRI GGCC 4 cut(s) 113, 1014, 1079, 1209
BtgI CCRYGG 3 cut(s) 777, 1074, 1204
BtsCI GGATG 3 cut(s) 226, 492, 715
BtsIMutI CAGTG 3 cut(s) 397, 903, 974
Cac8I GCNNGC 2 cut(s) 654, 1016
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 2 cut(s) 231, 802
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 4 cut(s) 589, 830, 1175, 1201
CseI GACGC 3 cut(s) 26, 73, 95
Csp6I GTAC 1 cut(s) 1071
CviAII CATG 8 cut(s) 167, 194, 214, 232, 463, 719, 803, 1075
CviQI GTAC 1 cut(s) 1071
DdeI CTNAG 5 cut(s) 65, 389, 693, 726, 1187
DpnI GATC 7 cut(s) 200, 236, 260, 467, 793, 886, 1141
DpnII GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
DraI TTTAAA 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 1207
Eam1104I CTCTTC 3 cut(s) 183, 660, 926
EarI CTCTTC 3 cut(s) 183, 660, 926
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 1074
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 4 cut(s) 589, 830, 1175, 1201
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 942
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoO65I GGTNACC 1 cut(s) 161
EcoRI GAATTC 2 cut(s) 356, 410
EcoRII CCWGG 4 cut(s) 113, 826, 1132, 1164
EcoT14I CCWWGG 1 cut(s) 1074
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 1074
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 8 cut(s) 170, 197, 217, 235, 466, 722, 806, 1078
FaqI GGGAC 1 cut(s) 112
FatI CATG 8 cut(s) 166, 193, 213, 231, 462, 718, 802, 1074
FauI CCCGC 2 cut(s) 89, 137
FbaI TGATCA 1 cut(s) 198
FblI GTMKAC 1 cut(s) 479
FokI GGATG 3 cut(s) 233, 499, 722
FriOI GRGCYC 1 cut(s) 178
FspBI CTAG 5 cut(s) 483, 495, 557, 852, 1146
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 1 cut(s) 437
GsaI CCCAGC 2 cut(s) 106, 636
GsuI CTGGAG 5 cut(s) 128, 137, 998, 1071, 1187
HaeIII GGCC 4 cut(s) 113, 1014, 1079, 1209
HapII CCGG 1 cut(s) 362
HgaI GACGC 3 cut(s) 26, 73, 95
HhaI GCGC 1 cut(s) 438
Hin1II CATG 8 cut(s) 170, 197, 217, 235, 466, 722, 806, 1078
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HincII GTYRAC 2 cut(s) 454, 480
HindII GTYRAC 2 cut(s) 454, 480
HinfI GANTC 8 cut(s) 376, 450, 740, 806, 863, 913, 959, 985
HpaII CCGG 1 cut(s) 362
HphI GGTGA 5 cut(s) 79, 263, 406, 980, 994
Hpy166II GTNNAC 3 cut(s) 271, 454, 480
Hpy188I TCNGA 8 cut(s) 136, 552, 696, 796, 811, 912, 1003, 1188
Hpy8I GTNNAC 3 cut(s) 271, 454, 480
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 3 cut(s) 24, 1110, 1143
HpyCH4III ACNGT 6 cut(s) 91, 477, 786, 824, 907, 1042
HpyCH4V TGCA 7 cut(s) 249, 652, 722, 746, 755, 767, 956
HpyF3I CTNAG 5 cut(s) 65, 389, 693, 726, 1187
Hsp92II CATG 8 cut(s) 170, 197, 217, 235, 466, 722, 806, 1078
HspAI GCGC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 6 cut(s) 33, 137, 147, 153, 205, 364
LweI GCATC 4 cut(s) 329, 477, 950, 994
MaeI CTAG 5 cut(s) 483, 495, 557, 852, 1146
MaeIII GTNAC 7 cut(s) 85, 161, 380, 968, 982, 1036, 1055
MalI GATC 7 cut(s) 200, 236, 260, 467, 793, 886, 1141
MbiI CCGCTC 2 cut(s) 132, 419
MboI GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
MfeI CAATTG 1 cut(s) 442
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 4 cut(s) 144, 178, 397, 900
MlsI TGGCCA 1 cut(s) 1209
MluCI AATT 8 cut(s) 305, 356, 410, 442, 525, 538, 878, 926
MluNI TGGCCA 1 cut(s) 1209
MlyI GAGTC 5 cut(s) 459, 749, 872, 968, 979
Mox20I TGGCCA 1 cut(s) 1209
MroXI GAANNNNTTC 1 cut(s) 267
MscI TGGCCA 1 cut(s) 1209
MseI TTAA 3 cut(s) 519, 660, 1160
Msp20I TGGCCA 1 cut(s) 1209
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 5 cut(s) 115, 362, 828, 1134, 1166
MunI CAATTG 1 cut(s) 442
MvaI CCWGG 4 cut(s) 115, 828, 1134, 1166
MvnI CGCG 1 cut(s) 84
NciI CCSGG 1 cut(s) 362
NcoI CCATGG 1 cut(s) 1074
NdeII GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
NlaIII CATG 8 cut(s) 170, 197, 217, 235, 466, 722, 806, 1078
NlaIV GGNNCC 3 cut(s) 35, 574, 1202
NmuCI GTSAC 5 cut(s) 85, 968, 982, 1036, 1055
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 2 cut(s) 197, 217
PagI TCATGA 2 cut(s) 231, 802
PciSI GCTCTTC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 3 cut(s) 376, 806, 913
PfoI TCCNGGA 1 cut(s) 360
PleI GAGTC 5 cut(s) 458, 748, 871, 967, 979
PpsI GAGTC 5 cut(s) 458, 748, 871, 967, 979
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 4 cut(s) 113, 826, 1132, 1164
PspEI GGTNACC 1 cut(s) 161
PspFI CCCAGC 2 cut(s) 102, 632
PspGI CCWGG 4 cut(s) 113, 826, 1132, 1164
PspN4I GGNNCC 3 cut(s) 35, 574, 1202
PspPI GGNCC 4 cut(s) 589, 830, 1175, 1201
PstI CTGCAG 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 1 cut(s) 791
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 1 cut(s) 1072
RsaNI GTAC 1 cut(s) 1071
SacI GAGCTC 1 cut(s) 178
SalI GTCGAC 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 3 cut(s) 519, 660, 1160
Sau3AI GATC 7 cut(s) 198, 234, 258, 465, 791, 884, 1139
Sau96I GGNCC 4 cut(s) 589, 830, 1175, 1201
SchI GAGTC 5 cut(s) 459, 749, 872, 968, 979
ScrFI CCNGG 5 cut(s) 115, 362, 828, 1134, 1166
SduI GDGCHC 4 cut(s) 144, 178, 397, 900
SfaNI GCATC 4 cut(s) 329, 477, 950, 994
SfcI CTRYAG 1 cut(s) 744
SinI GGWCC 4 cut(s) 589, 830, 1175, 1201
Sse9I AATT 8 cut(s) 305, 356, 410, 442, 525, 538, 878, 926
SsiI CCGC 5 cut(s) 45, 82, 130, 417, 447
SspMI CTAG 5 cut(s) 483, 495, 557, 852, 1146
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 5 cut(s) 113, 360, 826, 1132, 1164
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 6 cut(s) 91, 477, 786, 824, 907, 1042
TaqI TCGA 9 cut(s) 15, 184, 237, 261, 323, 344, 479, 534, 1128
TaqII GACCGA 1 cut(s) 186
TasI AATT 8 cut(s) 305, 356, 410, 442, 525, 538, 878, 926
TfiI GAWTC 3 cut(s) 376, 806, 913
Tru1I TTAA 3 cut(s) 519, 660, 1160
Tru9I TTAA 3 cut(s) 519, 660, 1160
TscAI CASTG 3 cut(s) 397, 910, 981
TseFI GTSAC 5 cut(s) 85, 968, 982, 1036, 1055
Tsp45I GTSAC 5 cut(s) 85, 968, 982, 1036, 1055
TspDTI ATGAA 2 cut(s) 524, 1077
TspGWI ACGGA 3 cut(s) 565, 686, 1193
TspRI CASTG 3 cut(s) 397, 910, 981
VpaK11BI GGWCC 4 cut(s) 589, 830, 1175, 1201
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 3 cut(s) 356, 410, 878
XbaI TCTAGA 1 cut(s) 1145
XceI RCATGY 2 cut(s) 197, 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 824
XmiI GTMKAC 1 cut(s) 479
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 5 cut(s) 483, 495, 557, 852, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.