FvH4_4g27660

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012396
Physical Location & Seq
Forward (+)
28552458 .. 28558049
5592 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27660.t1

Sequence Viewer

Length: 1209 bp
ATGGGAAGAAAGCTCGACGCTTTGTTTGGTAGGAGCAAGACCTCTAAGTTGAAACCAGTCCTCGCTCTTGCTCTCTCCCGCCTTGTCGTCCTCAAAACGCAGCGTCAGGCCAGGTGCAGCCAGTCCCGCTCCGATGTGCTCCAGCTCCTCCAGCTCGGCCACCATGACCGAGCTCTTATTCGAGTTGAGCAGGTGATCAAGGAACAGAACATGCTGGATGTGTATGTCATGATCGAGCGGTATTGCAACCTCTTGATCGAAAGAGTTCACCTCATTGAACAAGAGAGGGAATGTCCAGAGGAATTGAGGGAGGCAGCATCAAGTATGCTGTATGCTGCTTCGAGATGTGGAGAATTCCCAGAGCTTCAAGAGATTCGTAGTATTCTCAGCGCTCGCTTTGGCAAGGAATTCACTGCTCGTTCCATTGAATTGCGCAACAATTGCGGAGTCAGCCTTATCATGACCCAAAAACTGTCGACTAGGATGCCATCCAGGGAGAGCAGAATGAAAGTGCTTAAAGAAATTGCCTTTGATAATTCCATTGTTCTGAACCTAGAAGAAACTTCTGGCTCCGTTGAGGATAAGAAGGTCCAGACACCACCACACCCACAGGCTAGTTCAAGTGGCACGGCAGGATCAGCAGAAGTTCTGCAGGCTTTTAAAGAGGAGACGGAAAAAGATGATAAGTACTCTAATAAGAAATACAAGGATGTGGCTCATGCAGCTCAAGCTGCTTTTGAGTCTGCAGCTTATGCAGCGGCAGCTGCAAGAGCAGCCGTGGAACTGTCCAGATCTGACACTCATGATTCTGATGATCAAAACAGTCCTGGTCCTAAACGAGGCAAATTATCAAGCAGATATATATCCTCAAAAACTGAATTCAGATCACAGAATGAGCACATCACTGTGCAGAATCAGGCTGAAGTGAAGAGGTCAGCATCTTCAAGTTCAGATTCAGAAGGTGATGTATCAGAGGTGACCCCTCCAGTGTCCTCGGATGCCACAGGCCAGCCCAGTCATTCAGGGAAGGATACAATTTTTGATGATAGTGAAGATGAACAAAGTACCAAGGCCCCATCTCCTAAGGGAGTTCCTTCAAGGGTTCAATCTGGGATGAAGGTCGAGCCGGAGGGATCATCTAGAAGGCTAACATTAAACTTAGAGAAGGGACCATTCTCTCTGAGAAACAGACGGGTCTTTGGACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

403

Amino Acids

44.54

Weight (kDa)

8.46

Isoelectric Point (pI)

66.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 1e-53 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 181
Acc16I TGCGCA 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 181
AccBSI CCGCTC 2 cut(s) 129, 238
AccI GTMKAC 1 cut(s) 476
AciI CCGC 5 cut(s) 79, 127, 238, 444, 758
AclWI GGATC 2 cut(s) 643, 1141
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 3 cut(s) 353, 407, 878
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 2 cut(s) 689, 1066
AfeI AGCGCT 1 cut(s) 391
AfiI CCNNNNNNNGG 1 cut(s) 839
AgsI TTSAA 8 cut(s) 52, 278, 368, 428, 621, 945, 1098, 1106
AjnI CCWGG 3 cut(s) 110, 491, 826
AluBI AGCT 9 cut(s) 13, 145, 154, 173, 364, 725, 731, 749, 764
AluI AGCT 9 cut(s) 13, 145, 154, 173, 364, 725, 731, 749, 764
Alw21I GWGCWC 3 cut(s) 141, 175, 900
Alw26I GTCTC 1 cut(s) 662
AlwI GGATC 2 cut(s) 643, 1141
Aor51HI AGCGCT 1 cut(s) 391
AoxI GGCC 4 cut(s) 108, 157, 1006, 1071
ApoI RAATTY 3 cut(s) 353, 407, 878
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 392, 435
AspS9I GGNCC 4 cut(s) 589, 830, 1072, 1169
AsuHPI GGTGA 4 cut(s) 205, 260, 974, 988
AvaII GGWCC 3 cut(s) 589, 830, 1169
AxyI CCTNAGG 1 cut(s) 1083
BanII GRGCYC 1 cut(s) 175
Bbv12I GWGCWC 3 cut(s) 141, 175, 900
BccI CCATC 2 cut(s) 496, 1084
BceAI ACGGC 2 cut(s) 645, 761
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BciT130I CCWGG 3 cut(s) 112, 493, 828
BciVI GTATCC 1 cut(s) 1024
BclI TGATCA 2 cut(s) 195, 814
BcoDI GTCTC 1 cut(s) 662
BfaI CTAG 4 cut(s) 480, 554, 615, 1140
BfmI CTRYAG 2 cut(s) 650, 744
BfoI RGCGCY 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 181
BfuI GTATCC 1 cut(s) 1024
BglII AGATCT 1 cut(s) 791
BmcAI AGTACT 1 cut(s) 689
Bme1390I CCNGG 3 cut(s) 112, 493, 828
Bme18I GGWCC 3 cut(s) 589, 830, 1169
BmgT120I GGNCC 4 cut(s) 589, 830, 1072, 1169
BmiI GGNNCC 3 cut(s) 571, 1074, 1170
BmrFI CCNGG 3 cut(s) 112, 493, 828
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 4 cut(s) 326, 474, 947, 988
BmuI ACTGGG 1 cut(s) 1008
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 3 cut(s) 125, 134, 969
BpuEI CTTGAG 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 862
BsaJI CCNNGG 4 cut(s) 492, 777, 993, 1068
Bsc4I CCNNNNNNNGG 1 cut(s) 839
Bse1I ACTGG 4 cut(s) 56, 121, 986, 1014
Bse21I CCTNAGG 1 cut(s) 1083
Bse8I GATNNNNATC 1 cut(s) 862
BseBI CCWGG 3 cut(s) 112, 493, 828
BseDI CCNNGG 4 cut(s) 492, 777, 993, 1068
BseGI GGATG 6 cut(s) 223, 488, 489, 715, 1003, 1119
BseJI GATNNNNATC 1 cut(s) 862
BseLI CCNNNNNNNGG 1 cut(s) 839
BseMII CTCAG 2 cut(s) 400, 1172
BseNI ACTGG 4 cut(s) 56, 121, 986, 1014
BseRI GAGGAG 2 cut(s) 137, 680
BsgI GTGCAG 2 cut(s) 136, 929
BshFI GGCC 4 cut(s) 110, 159, 1008, 1073
BsiHKAI GWGCWC 3 cut(s) 141, 175, 900
BsiSI CCGG 1 cut(s) 1127
BslFI GGGAC 2 cut(s) 109, 1182
BslI CCNNNNNNNGG 1 cut(s) 839
BsmAI GTCTC 1 cut(s) 662
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 2 cut(s) 109, 1182
BsnI GGCC 4 cut(s) 110, 159, 1008, 1073
Bsp1286I GDGCHC 3 cut(s) 141, 175, 900
Bsp143I GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
BspACI CCGC 5 cut(s) 79, 127, 238, 444, 758
BspANI GGCC 4 cut(s) 110, 159, 1008, 1073
BspCNI CTCAG 2 cut(s) 399, 1173
BspHI TCATGA 3 cut(s) 228, 459, 802
BspLI GGNNCC 3 cut(s) 571, 1074, 1170
BspMAI CTGCAG 2 cut(s) 654, 748
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 2 cut(s) 643, 1141
BsrBI CCGCTC 2 cut(s) 129, 238
BsrI ACTGG 4 cut(s) 56, 121, 986, 1014
BssECI CCNNGG 4 cut(s) 492, 777, 993, 1068
BssMI GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
BssT1I CCWWGG 1 cut(s) 1068
Bst2UI CCWGG 3 cut(s) 112, 493, 828
Bst4CI ACNGT 4 cut(s) 474, 786, 824, 907
Bst6I CTCTTC 1 cut(s) 923
BstAPI GCANNNNNTGC 2 cut(s) 441, 752
BstC8I GCNNGC 3 cut(s) 394, 654, 1010
BstDEI CTNAG 5 cut(s) 45, 386, 1083, 1159, 1181
BstDSI CCRYGG 1 cut(s) 777
BstEII GGTNACC 1 cut(s) 976
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 6 cut(s) 223, 488, 489, 715, 1003, 1119
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 2 cut(s) 392, 435
BstKTI GATC 8 cut(s) 198, 234, 258, 638, 794, 817, 887, 1136
BstMAI GTCTC 1 cut(s) 662
BstMBI GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
BstNI CCWGG 3 cut(s) 112, 493, 828
BstNSI RCATGY 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 976
BstSCI CCNGG 3 cut(s) 110, 491, 826
BstSFI CTRYAG 2 cut(s) 650, 744
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
Bsu36I CCTNAGG 1 cut(s) 1083
BsuI GTATCC 1 cut(s) 1024
BsuRI GGCC 4 cut(s) 110, 159, 1008, 1073
BtgI CCRYGG 1 cut(s) 777
BtsCI GGATG 6 cut(s) 223, 488, 489, 715, 1003, 1119
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 4 cut(s) 411, 903, 993, 1204
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 3 cut(s) 394, 654, 1010
CciI TCATGA 3 cut(s) 228, 459, 802
CfoI GCGC 2 cut(s) 392, 435
Cfr13I GGNCC 4 cut(s) 589, 830, 1072, 1169
CseI GACGC 2 cut(s) 26, 92
Csp6I GTAC 2 cut(s) 688, 1065
CviAII CATG 6 cut(s) 164, 211, 229, 460, 719, 803
CviQI GTAC 2 cut(s) 688, 1065
DdeI CTNAG 5 cut(s) 45, 386, 1083, 1159, 1181
DpnI GATC 8 cut(s) 197, 233, 257, 637, 793, 816, 886, 1135
DpnII GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
DraI TTTAAA 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 923
EarI CTCTTC 1 cut(s) 923
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 1068
Eco24I GRGCYC 1 cut(s) 175
Eco47I GGWCC 3 cut(s) 589, 830, 1169
Eco47III AGCGCT 1 cut(s) 391
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 942
Eco81I CCTNAGG 1 cut(s) 1083
Eco91I GGTNACC 1 cut(s) 976
EcoICRI GAGCTC 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoO109I RGGNCCY 1 cut(s) 1072
EcoO65I GGTNACC 1 cut(s) 976
EcoRI GAATTC 3 cut(s) 353, 407, 878
EcoRII CCWGG 3 cut(s) 110, 491, 826
EcoT14I CCWWGG 1 cut(s) 1068
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 1068
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 6 cut(s) 167, 214, 232, 463, 722, 806
FaqI GGGAC 2 cut(s) 109, 1182
FatI CATG 6 cut(s) 163, 210, 228, 459, 718, 802
FauI CCCGC 2 cut(s) 86, 134
FbaI TGATCA 2 cut(s) 195, 814
FblI GTMKAC 1 cut(s) 476
FokI GGATG 6 cut(s) 230, 475, 496, 722, 1010, 1126
FriOI GRGCYC 1 cut(s) 175
FspBI CTAG 4 cut(s) 480, 554, 615, 1140
FspI TGCGCA 1 cut(s) 434
GlaI GCGC 2 cut(s) 391, 434
GsuI CTGGAG 3 cut(s) 125, 134, 969
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 4 cut(s) 110, 159, 1008, 1073
HapII CCGG 1 cut(s) 1127
HgaI GACGC 2 cut(s) 26, 92
HhaI GCGC 2 cut(s) 392, 435
Hin1II CATG 6 cut(s) 167, 214, 232, 463, 722, 806
Hin6I GCGC 2 cut(s) 390, 433
HinP1I GCGC 2 cut(s) 390, 433
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HinfI GANTC 6 cut(s) 373, 447, 740, 806, 913, 953
HpaII CCGG 1 cut(s) 1127
HphI GGTGA 4 cut(s) 205, 260, 974, 988
Hpy166II GTNNAC 2 cut(s) 268, 477
Hpy8I GTNNAC 2 cut(s) 268, 477
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 7 cut(s) 580, 953, 1021, 1104, 1111, 1137, 1159
HpyCH4III ACNGT 4 cut(s) 474, 786, 824, 907
HpyCH4V TGCA 8 cut(s) 117, 246, 652, 722, 746, 755, 767, 910
HpyF3I CTNAG 5 cut(s) 45, 386, 1083, 1159, 1181
Hsp92II CATG 6 cut(s) 167, 214, 232, 463, 722, 806
HspAI GCGC 2 cut(s) 390, 433
Ksp22I TGATCA 2 cut(s) 195, 814
Kzo9I GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
LmnI GCTCC 5 cut(s) 33, 134, 144, 150, 575
LweI GCATC 4 cut(s) 326, 474, 947, 988
MaeI CTAG 4 cut(s) 480, 554, 615, 1140
MaeIII GTNAC 1 cut(s) 976
MalI GATC 8 cut(s) 197, 233, 257, 637, 793, 816, 886, 1135
MbiI CCGCTC 2 cut(s) 129, 238
MboI GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
MboII GAAGA 5 cut(s) 18, 569, 933, 940, 1064
MfeI CAATTG 1 cut(s) 439
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 3 cut(s) 141, 175, 900
MlyI GAGTC 2 cut(s) 456, 749
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 3 cut(s) 516, 660, 1154
MslI CAYNNNNRTG 1 cut(s) 905
MspA1I CMGCKG 2 cut(s) 758, 764
MspI CCGG 1 cut(s) 1127
MspR9I CCNGG 3 cut(s) 112, 493, 828
MunI CAATTG 1 cut(s) 439
MvaI CCWGG 3 cut(s) 112, 493, 828
NdeII GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
NlaIII CATG 6 cut(s) 167, 214, 232, 463, 722, 806
NlaIV GGNNCC 3 cut(s) 571, 1074, 1170
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 976
NsbI TGCGCA 1 cut(s) 434
NspI RCATGY 1 cut(s) 214
PagI TCATGA 3 cut(s) 228, 459, 802
PaqCI CACCTGC 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 4 cut(s) 373, 806, 913, 953
PleI GAGTC 2 cut(s) 455, 748
PpsI GAGTC 2 cut(s) 455, 748
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 3 cut(s) 110, 491, 826
PspEI GGTNACC 1 cut(s) 976
PspGI CCWGG 3 cut(s) 110, 491, 826
PspN4I GGNNCC 3 cut(s) 571, 1074, 1170
PspPI GGNCC 4 cut(s) 589, 830, 1072, 1169
PstI CTGCAG 2 cut(s) 654, 748
PsuI RGATCY 1 cut(s) 791
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 2 cut(s) 689, 1066
RsaNI GTAC 2 cut(s) 688, 1065
RseI CAYNNNNRTG 1 cut(s) 905
SacI GAGCTC 1 cut(s) 175
SalI GTCGAC 1 cut(s) 475
SaqAI TTAA 3 cut(s) 516, 660, 1154
Sau3AI GATC 8 cut(s) 195, 231, 255, 635, 791, 814, 884, 1133
Sau96I GGNCC 4 cut(s) 589, 830, 1072, 1169
ScaI AGTACT 1 cut(s) 689
SchI GAGTC 2 cut(s) 456, 749
ScrFI CCNGG 3 cut(s) 112, 493, 828
SduI GDGCHC 3 cut(s) 141, 175, 900
SfaNI GCATC 4 cut(s) 326, 474, 947, 988
SfcI CTRYAG 2 cut(s) 650, 744
SinI GGWCC 3 cut(s) 589, 830, 1169
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
SsiI CCGC 5 cut(s) 79, 127, 238, 444, 758
SspMI CTAG 4 cut(s) 480, 554, 615, 1140
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 3 cut(s) 110, 491, 826
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 4 cut(s) 474, 786, 824, 907
TaqI TCGA 7 cut(s) 15, 181, 234, 258, 341, 476, 1122
TaqII GACCGA 1 cut(s) 183
TatI WGTACW 1 cut(s) 687
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 4 cut(s) 373, 806, 913, 953
Tru1I TTAA 3 cut(s) 516, 660, 1154
Tru9I TTAA 3 cut(s) 516, 660, 1154
TscAI CASTG 3 cut(s) 418, 910, 993
TseFI GTSAC 1 cut(s) 976
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 3 cut(s) 521, 1071, 1130
TspGWI ACGGA 2 cut(s) 562, 686
TspRI CASTG 3 cut(s) 418, 910, 993
VpaK11BI GGWCC 3 cut(s) 589, 830, 1169
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 3 cut(s) 353, 407, 878
XbaI TCTAGA 1 cut(s) 1139
XceI RCATGY 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 4 cut(s) 480, 554, 615, 1140
ZrmI AGTACT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.