AT1G13380

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010463
Physical Location & Seq
Reverse (-)
4588994 .. 4590501
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G13380.1

Sequence Viewer

Length: 567 bp
ATGGGAGAAGGGAAGGCCTCCACTCTGGTTTTCATCCTCGTCGTCGCTCTTAGTTTAGTAGCCTTCGGCTTCTCCATCGCCGCCGAGCGCCGCCGCAGCATCGGGAAATCTATTCAAGACCCAATAACAAACACAACGTTCTGCGTTTATGACTCTGATGTAGCCACTGGTTATGGAGTTGGAGCTTTCCTTTTCCTCCTCTCTAGTGAATCATTGCTTATGAGTGTTACCAAATGCATGTGTTTTGGAAGACCCTTAGCCCCTGGAAGTGACCGTGCTTGGTCTATTATCTATTTCATCTCTTCGTGGATGACATTTCTGGTAGCAGAAGCTTGTGTAATAGCAGGAGCGACCAAGAACGCATACCACACAAAGTACTTAAGTTCGCAAACCTTTTCGTGTGCATCATTGCGTAAAGGAATTTTCATTGCAGGTGCAGTCTTTATAGTTGCTACTATGGTTCTTAATGTCTACTACTACATGTATTTTACCAAGTCCGTCTCTTCGCCGCCTGCTCACAAAGCCAACCGCTCTAGTTCCAATATTGGTATGGCGGGTTATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

188

Amino Acids

20.26

Weight (kDa)

9.26

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 62 - 153 1.3e-24 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 422
Acc36I ACCTGC 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 531
AccI GTMKAC 1 cut(s) 471
AciI CCGC 6 cut(s) 81, 91, 94, 509, 529, 554
AclI AACGTT 1 cut(s) 137
AcsI RAATTY 1 cut(s) 420
AfaI GTAC 1 cut(s) 377
AflII CTTAAG 1 cut(s) 379
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 1 cut(s) 116
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 2 cut(s) 185, 332
AluI AGCT 2 cut(s) 185, 332
Alw26I GTCTC 1 cut(s) 505
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 1 cut(s) 420
AspLEI GCGC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 83
BciT130I CCWGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 2 cut(s) 204, 534
BfoI RGCGCY 1 cut(s) 91
BfrI CTTAAG 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 5 cut(s) 81, 91, 94, 97, 509
BlsI GCNGC 5 cut(s) 82, 92, 95, 98, 510
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 108, 413
BpiI GAAGAC 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 256
BsaJI CCNNGG 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 3 cut(s) 212, 407, 426
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 2 cut(s) 33, 315
BseMI GCAATG 3 cut(s) 212, 407, 426
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 1 cut(s) 108
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 505
BsmBI CGTCTC 1 cut(s) 505
BsnI GGCC 1 cut(s) 17
BspACI CCGC 6 cut(s) 81, 91, 94, 509, 529, 554
BspANI GGCC 1 cut(s) 17
BspMI ACCTGC 1 cut(s) 422
BspTI CTTAAG 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 531
BsrDI GCAATG 3 cut(s) 212, 407, 426
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 2 cut(s) 307, 508
BstAFI CTTAAG 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 2 cut(s) 50, 256
BstF5I GGATG 2 cut(s) 33, 315
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstMAI GTCTC 1 cut(s) 505
BstMWI GCNNNNNNNGC 3 cut(s) 96, 521, 560
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 2 cut(s) 241, 484
BstSCI CCNGG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 17
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 2 cut(s) 33, 315
BtsIMutI CAGTG 1 cut(s) 165
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 513
CfoI GCGC 1 cut(s) 90
Csp6I GTAC 1 cut(s) 376
CviAII CATG 2 cut(s) 238, 481
CviJI RGCY 8 cut(s) 17, 62, 69, 164, 185, 260, 332, 524
CviKI_1 RGCY 8 cut(s) 17, 62, 69, 164, 185, 260, 332, 524
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 50, 256
Eam1104I CTCTTC 2 cut(s) 307, 508
EarI CTCTTC 2 cut(s) 307, 508
Eco147I AGGCCT 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 262
EcoT22I ATGCAT 2 cut(s) 239, 565
Esp3I CGTCTC 1 cut(s) 505
FaeI CATG 2 cut(s) 241, 484
FatI CATG 2 cut(s) 237, 480
FauI CCCGC 1 cut(s) 547
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 5 cut(s) 81, 91, 94, 97, 509
FokI GGATG 2 cut(s) 20, 322
Fsp4HI GCNGC 5 cut(s) 81, 91, 94, 97, 509
FspBI CTAG 2 cut(s) 204, 534
GlaI GCGC 1 cut(s) 89
GluI GCNGC 5 cut(s) 81, 91, 94, 97, 509
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 1 cut(s) 17
HhaI GCGC 1 cut(s) 90
Hin1II CATG 2 cut(s) 241, 484
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 2 cut(s) 152, 209
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 2 cut(s) 103, 116
Hpy8I GTNNAC 1 cut(s) 472
Hpy99I CGWCG 2 cut(s) 44, 47
HpyAV CCTTC 2 cut(s) 7, 73
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 5 cut(s) 237, 404, 431, 437, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 521, 560
HpyF3I CTNAG 2 cut(s) 50, 256
HpySE526I ACGT 1 cut(s) 137
Hsp92II CATG 2 cut(s) 241, 484
HspAI GCGC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 182, 347
LpnPI CCDG 8 cut(s) 11, 153, 249, 276, 305, 330, 417, 525
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 2 cut(s) 108, 413
MaeI CTAG 2 cut(s) 204, 534
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 2 cut(s) 226, 269
MbiI CCGCTC 1 cut(s) 531
MboII GAAGA 3 cut(s) 261, 294, 495
MluCI AATT 1 cut(s) 420
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 4 cut(s) 28, 47, 206, 209
Mph1103I ATGCAT 2 cut(s) 239, 565
MseI TTAA 2 cut(s) 380, 465
MspCI CTTAAG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 96, 521, 560
NlaIII CATG 2 cut(s) 241, 484
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 269
NsiI ATGCAT 2 cut(s) 239, 565
NspI RCATGY 2 cut(s) 241, 484
PaqCI CACCTGC 1 cut(s) 422
PceI AGGCCT 1 cut(s) 17
PciI ACATGT 1 cut(s) 480
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 5 cut(s) 82, 92, 95, 98, 510
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PscI ACATGT 1 cut(s) 480
Psp1406I AACGTT 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SaqAI TTAA 2 cut(s) 380, 465
SatI GCNGC 5 cut(s) 81, 91, 94, 97, 509
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 264
SetI ASST 5 cut(s) 140, 187, 334, 395, 436
SfaNI GCATC 2 cut(s) 108, 413
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 1 cut(s) 420
SseBI AGGCCT 1 cut(s) 17
SsiI CCGC 6 cut(s) 81, 91, 94, 509, 529, 554
SspI AATATT 1 cut(s) 544
SspMI CTAG 2 cut(s) 204, 534
StuI AGGCCT 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 1 cut(s) 140
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 375
TauI GCSGC 4 cut(s) 83, 93, 96, 511
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 380, 465
Tru9I TTAA 2 cut(s) 380, 465
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 3 cut(s) 22, 286, 415
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 1 cut(s) 172
Vha464I CTTAAG 1 cut(s) 379
XapI RAATTY 1 cut(s) 420
XceI RCATGY 2 cut(s) 241, 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 471
XspI CTAG 2 cut(s) 204, 534
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 2 cut(s) 239, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.