RchiOBHm_Chr2g0089361

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010463
Physical Location & Seq
Reverse (-)
3531686 .. 3532199
514 bp
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UTR
Exon/CDS
Intron
PRQ46466

Sequence Viewer

Length: 417 bp
ATGGGAGAAGGAAAAGGCTCGACTCTGGTGCACCTTCTGGTCGTCGTTCTCAGCTTAGTGGCTTTTGGGTTCGCCATTGCCGCTGAGAGACGAAGAAGTGTGGGTGTAATATATAAGGATGGACGAACAAATGAAACTTACTGTGTCTGTAACTCAGATGTTGCTACTGGTTATGGAGTGGGCGGCTTCTTGTTTCTTCTCTCGGGGGAATCCCTACTCATGGGTGTCACGAAGTGCATGTGTTTTGGAAGACCCTTAGCCCCTGGCGGAAATCGAGCTTGGGCCATTATTTATTTTGTCTCATCATTGGTAACTTTTCTGATTGCAGAATCATGCTTGATTGGTGGTGCTACAAAAAATGCCTATCACACCAAGTACCGGGGGATGATATATGCTCAGAACTTTTCCTGTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

138

Amino Acids

14.77

Weight (kDa)

8.99

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 63 - 137 4.2e-17 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 81, 183, 267
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 2 cut(s) 377, 412
AfiI CCNNNNNNNGG 2 cut(s) 220, 378
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 2 cut(s) 54, 278
AluI AGCT 2 cut(s) 54, 278
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 82, 304
Alw44I GTGCAC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 1 cut(s) 282
ApaLI GTGCAC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 282
AsuC2I CCSGG 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 1 cut(s) 113
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 82, 304
BisI GCNGC 2 cut(s) 81, 184
BlsI GCNGC 2 cut(s) 82, 185
Bme1390I CCNGG 2 cut(s) 264, 380
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 282
BmrFI CCNGG 2 cut(s) 264, 380
BpiI GAAGAC 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 262, 379
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 378
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 262, 379
BseGI GGATG 2 cut(s) 124, 390
BseLI CCNNNNNNNGG 2 cut(s) 220, 378
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 4 cut(s) 64, 75, 168, 410
BseNI ACTGG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 33
BshFI GGCC 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 220, 378
BsmAI GTCTC 2 cut(s) 82, 304
BsmBI CGTCTC 1 cut(s) 82
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 33
BspACI CCGC 3 cut(s) 81, 183, 267
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 4 cut(s) 63, 76, 167, 409
BsrDI GCAATG 1 cut(s) 75
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 262, 379
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 414
BstDEI CTNAG 6 cut(s) 50, 55, 84, 154, 256, 396
BstF5I GGATG 2 cut(s) 124, 390
BstMAI GTCTC 2 cut(s) 82, 304
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 2 cut(s) 262, 378
BstSLI GKGCMC 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 284
BtsCI GGATG 2 cut(s) 124, 390
Cfr13I GGNCC 1 cut(s) 282
Csp6I GTAC 2 cut(s) 376, 411
CviAII CATG 3 cut(s) 220, 238, 333
CviJI RGCY 7 cut(s) 18, 54, 62, 186, 260, 278, 284
CviKI_1 RGCY 7 cut(s) 18, 54, 62, 186, 260, 278, 284
CviQI GTAC 2 cut(s) 376, 411
DdeI CTNAG 6 cut(s) 50, 55, 84, 154, 256, 396
DraIII CACNNNGTG 1 cut(s) 234
EciI GGCGGA 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 223, 241, 336
FaiI YATR 8 cut(s) 112, 114, 174, 221, 239, 334, 391, 393
FatI CATG 3 cut(s) 219, 237, 332
Fnu4HI GCNGC 2 cut(s) 81, 184
FokI GGATG 2 cut(s) 131, 397
Fsp4HI GCNGC 2 cut(s) 81, 184
GluI GCNGC 2 cut(s) 81, 184
HaeIII GGCC 1 cut(s) 284
HapII CCGG 1 cut(s) 379
Hin1II CATG 3 cut(s) 223, 241, 336
HinfI GANTC 3 cut(s) 22, 209, 329
HpaII CCGG 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 157, 321, 399
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 3 cut(s) 31, 237, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 6 cut(s) 50, 55, 84, 154, 256, 396
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 3 cut(s) 223, 241, 336
LpnPI CCDG 6 cut(s) 11, 23, 153, 249, 276, 392
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 3 cut(s) 149, 226, 310
MboII GAAGA 3 cut(s) 105, 188, 261
MhlI GDGCHC 1 cut(s) 33
MlyI GAGTC 1 cut(s) 16
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 264, 380
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 1 cut(s) 80
NciI CCSGG 1 cut(s) 380
NlaIII CATG 3 cut(s) 223, 241, 336
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 241
PfeI GAWTC 2 cut(s) 209, 329
PkrI GCNGC 2 cut(s) 82, 185
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspPI GGNCC 1 cut(s) 282
RsaI GTAC 2 cut(s) 377, 412
RsaNI GTAC 2 cut(s) 376, 411
SatI GCNGC 2 cut(s) 81, 184
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 264, 380
SduI GDGCHC 1 cut(s) 33
SetI ASST 4 cut(s) 36, 56, 280, 416
SsiI CCGC 3 cut(s) 81, 183, 267
StyD4I CCNGG 2 cut(s) 262, 378
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 1 cut(s) 416
TaqI TCGA 2 cut(s) 20, 274
TauI GCSGC 2 cut(s) 83, 186
TfiI GAWTC 2 cut(s) 209, 329
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 147
VneI GTGCAC 1 cut(s) 29
XceI RCATGY 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.