RLG00000006614

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010463
Physical Location & Seq
Reverse (-)
9030452 .. 9033585
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006614

Sequence Viewer

Length: 663 bp
ATGGGAGAAGGAAAAGGCTCGACTCTGGTGCACCTTCTGGTCGTCGTTCTCAGCTTAGTGGCTTTTGGGTTCGCCATTGCCGCCGAGAGACGAAGAAGTGTGGGTGTAATATATAAGGATGAACGAACAAATGAAACTTACTGTGTCTATAACTCAGATGTCGCTACTGGTTATGGAGTGGGCGGCTTCTTGTTTCTTCTCTCGGGGGAGTCCTTACTCATGAGTGTCACGAAGTGCATGTGTTTTGGAAGACCCTTAGCCCCTGGTGGAAATCGAGCTTGGACCATCATATATTTTGTCTCATCATTGGTTACTTTTCTGATTGCAGAATCATGCTTGATTGCTGGTGCTACAAAAAATGCATATCACACCAAGTACCGGGGGATGATATATGCTCAGAACTTCTCCTGTGACGCATTGCGAAAAGGCGTTTTTATTGCTGGAGCAGTATTTGTGGTTGCAACAATGGTCCTCAACGTCTATTACTACATGTACTTCACCAAGGCAATCACTACTCAGGCAGCTCACAAAGCAAAGCGTACAAGCTCAAACATCGGGATGGCTGGAGTATGGGATGCCTATGTGATTGCAGACTTGGGGCGGTTCAGGATAGAATTGGGTGTTCCTTTGCAAATGGCCACATACTGCCAGGGGCACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

221

Amino Acids

24.04

Weight (kDa)

9.22

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 63 - 156 2.3e-24 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 654
AciI CCGC 3 cut(s) 81, 183, 601
AcoI YGGCCR 1 cut(s) 636
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 3 cut(s) 377, 494, 541
AfiI CCNNNNNNNGG 1 cut(s) 378
AflIII ACRYGT 1 cut(s) 489
AjnI CCWGG 2 cut(s) 262, 648
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 4 cut(s) 54, 278, 524, 546
AluI AGCT 4 cut(s) 54, 278, 524, 546
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 82, 304
Alw44I GTGCAC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 1 cut(s) 636
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 282, 469
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 202
AvaII GGWCC 2 cut(s) 282, 469
BaeGI GKGCMC 2 cut(s) 33, 657
BalI TGGCCA 1 cut(s) 638
BanI GGYRCC 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 533
BccI CCATC 2 cut(s) 293, 553
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 2 cut(s) 264, 650
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 82, 304
BisI GCNGC 3 cut(s) 81, 184, 522
BlsI GCNGC 3 cut(s) 82, 185, 523
Bme1390I CCNGG 3 cut(s) 264, 380, 650
Bme18I GGWCC 2 cut(s) 282, 469
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 2 cut(s) 282, 469
BmiI GGNNCC 1 cut(s) 656
BmrFI CCNGG 3 cut(s) 264, 380, 650
BmsI GCATC 1 cut(s) 565
BpiI GAAGAC 1 cut(s) 256
BpmI CTGGAG 2 cut(s) 462, 585
Bpu10I CCTNAGC 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 380
BsaJI CCNNGG 4 cut(s) 262, 379, 501, 649
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 2 cut(s) 75, 416
BseBI CCWGG 2 cut(s) 264, 650
BseDI CCNNGG 4 cut(s) 262, 379, 501, 649
BseGI GGATG 4 cut(s) 124, 390, 564, 580
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMI GCAATG 2 cut(s) 75, 416
BseMII CTCAG 4 cut(s) 64, 168, 410, 530
BseNI ACTGG 1 cut(s) 172
BseSI GKGCMC 2 cut(s) 33, 657
BseXI GCAGC 1 cut(s) 533
BshFI GGCC 1 cut(s) 638
BshNI GGYRCC 1 cut(s) 654
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 2 cut(s) 82, 304
BsmBI CGTCTC 1 cut(s) 82
BsnI GGCC 1 cut(s) 638
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 2 cut(s) 33, 657
BspACI CCGC 3 cut(s) 81, 183, 601
BspANI GGCC 1 cut(s) 638
BspCNI CTCAG 4 cut(s) 63, 167, 409, 529
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 656
BspT107I GGYRCC 1 cut(s) 654
BsrDI GCAATG 2 cut(s) 75, 416
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 4 cut(s) 262, 379, 501, 649
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 2 cut(s) 264, 650
Bst4CI ACNGT 1 cut(s) 143
BstDEI CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
BstF5I GGATG 4 cut(s) 124, 390, 564, 580
BstMAI GTCTC 2 cut(s) 82, 304
BstMWI GCNNNNNNNGC 2 cut(s) 80, 530
BstNI CCWGG 2 cut(s) 264, 650
BstNSI RCATGY 2 cut(s) 241, 493
BstSCI CCNGG 3 cut(s) 262, 378, 648
BstSLI GKGCMC 2 cut(s) 33, 657
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 638
BtsCI GGATG 4 cut(s) 124, 390, 564, 580
CciI TCATGA 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 282, 469
CseI GACGC 1 cut(s) 422
Csp6I GTAC 3 cut(s) 376, 493, 540
CviAII CATG 4 cut(s) 220, 238, 333, 490
CviQI GTAC 3 cut(s) 376, 493, 540
DdeI CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
DraIII CACNNNGTG 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 282, 469
Eco88I CYCGRG 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 262, 648
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 4 cut(s) 223, 241, 336, 493
FatI CATG 4 cut(s) 219, 237, 332, 489
Fnu4HI GCNGC 3 cut(s) 81, 184, 522
FokI GGATG 4 cut(s) 131, 397, 571, 587
Fsp4HI GCNGC 3 cut(s) 81, 184, 522
GluI GCNGC 3 cut(s) 81, 184, 522
GsuI CTGGAG 2 cut(s) 462, 585
HaeIII GGCC 1 cut(s) 638
HapII CCGG 1 cut(s) 379
HgaI GACGC 1 cut(s) 422
Hin1II CATG 4 cut(s) 223, 241, 336, 493
HinfI GANTC 3 cut(s) 22, 209, 329
HpaII CCGG 1 cut(s) 379
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 157, 321, 399
Hpy188III TCNNGA 4 cut(s) 220, 229, 556, 607
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 7 cut(s) 31, 237, 326, 362, 461, 590, 631
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 530
HpyF3I CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 4 cut(s) 223, 241, 336, 493
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 1 cut(s) 565
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 3 cut(s) 226, 310, 410
MboII GAAGA 3 cut(s) 105, 188, 261
MhlI GDGCHC 2 cut(s) 33, 657
MlsI TGGCCA 1 cut(s) 638
MluCI AATT 1 cut(s) 614
MluNI TGGCCA 1 cut(s) 638
MlyI GAGTC 2 cut(s) 16, 218
MnlI CCTC 1 cut(s) 482
Mox20I TGGCCA 1 cut(s) 638
Mph1103I ATGCAT 1 cut(s) 364
MscI TGGCCA 1 cut(s) 638
MslI CAYNNNNRTG 1 cut(s) 557
Msp20I TGGCCA 1 cut(s) 638
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 3 cut(s) 264, 380, 650
MvaI CCWGG 2 cut(s) 264, 650
MwoI GCNNNNNNNGC 2 cut(s) 80, 530
NciI CCSGG 1 cut(s) 380
NlaIII CATG 4 cut(s) 223, 241, 336, 493
NlaIV GGNNCC 1 cut(s) 656
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 2 cut(s) 226, 410
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 2 cut(s) 241, 493
PagI TCATGA 1 cut(s) 219
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 1 cut(s) 329
PkrI GCNGC 3 cut(s) 82, 185, 523
PleI GAGTC 2 cut(s) 16, 217
PpsI GAGTC 2 cut(s) 16, 217
PscI ACATGT 1 cut(s) 489
Psp6I CCWGG 2 cut(s) 262, 648
PspGI CCWGG 2 cut(s) 262, 648
PspN4I GGNNCC 1 cut(s) 656
PspPI GGNCC 2 cut(s) 282, 469
RsaI GTAC 3 cut(s) 377, 494, 541
RsaNI GTAC 3 cut(s) 376, 493, 540
RseI CAYNNNNRTG 1 cut(s) 557
SatI GCNGC 3 cut(s) 81, 184, 522
Sau96I GGNCC 2 cut(s) 282, 469
SchI GAGTC 2 cut(s) 16, 218
ScrFI CCNGG 3 cut(s) 264, 380, 650
SduI GDGCHC 2 cut(s) 33, 657
SetI ASST 6 cut(s) 36, 56, 280, 480, 526, 548
SfaNI GCATC 1 cut(s) 565
SinI GGWCC 2 cut(s) 282, 469
SmiMI CAYNNNNRTG 1 cut(s) 557
Sse9I AATT 1 cut(s) 614
SsiI CCGC 3 cut(s) 81, 183, 601
StyD4I CCNGG 3 cut(s) 262, 378, 648
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 1 cut(s) 480
TaqI TCGA 2 cut(s) 20, 274
TasI AATT 1 cut(s) 614
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 83, 186
TfiI GAWTC 1 cut(s) 329
TseFI GTSAC 2 cut(s) 226, 410
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 226, 410
TspDTI ATGAA 2 cut(s) 135, 147
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 282, 469
XceI RCATGY 2 cut(s) 241, 493
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.