Rw4G029510

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010463
Physical Location & Seq
Forward (+)
56182016 .. 56186345
4330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029510.1

Sequence Viewer

Length: 639 bp
ATGTCAGTACAGCTTACTTTCCATATGCAAACTGGATTTAAGGATTTTTGGTTTAACTTTGTTTGGCAAATCTATGTTGACATTTTGGGATTAAGCTGGATTGTGGTGCTTTATGGTCTCAAATTTCTGTACCATCAATTTAAAGTAACCTTTGTTTCCTGGCAGGGTGTAATATATAAGGATGAACGAACAAATGAAACTTACTGTGTCTATAACTCAGATGTTGCTACTGGTTATGGAGTGGGCGGCTTCTTGTTTCTTCTCTCAGGGGAATCCCTACTCATGGGTGTCACGAAGTGCATGTGTTTTGGAAGACCCTTAGCCCCTGGCGGAAATCGAGCTTGGGCCATTATTTATTTTGTCTCATCATTGGTAACTTTTCTGATTGCAGAATCATGCTTGATTGCTGGTGCTACAAAAAATGCCTATCACACCAAGTACCGGGGGATGATATATGCTCAGAACTTCTCCTGTGACGCATTGCGAAAAGGCGTTTTTATTGCTGGAGCAGTATTTGTGGTTGCAACAATGGTCCTCAACGTCTATTACTACATGTACTTCACCAAGGCAATCACTACTCAGGCAGCTCACAAAGCAAAGCGTACAAGCTCAAACATCGGGATGGCTGGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

212

Amino Acids

23.88

Weight (kDa)

9.22

Isoelectric Point (pI)

25.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 84 - 177 8.8e-25 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 246, 330
AcsI RAATTY 1 cut(s) 122
AdeI CACNNNGTG 1 cut(s) 297
AfaI GTAC 5 cut(s) 9, 131, 440, 557, 604
AfiI CCNNNNNNNGG 2 cut(s) 283, 441
AflIII ACRYGT 1 cut(s) 552
AjnI CCWGG 2 cut(s) 158, 325
AjuI GAANNNNNNNTTGG 2 cut(s) 325, 357
AluBI AGCT 5 cut(s) 13, 96, 341, 587, 609
AluI AGCT 5 cut(s) 13, 96, 341, 587, 609
Alw26I GTCTC 2 cut(s) 122, 367
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 345, 532
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 1 cut(s) 553
AvaII GGWCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 596
BccI CCATC 2 cut(s) 141, 616
BciT130I CCWGG 2 cut(s) 160, 327
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 2 cut(s) 122, 367
BisI GCNGC 2 cut(s) 247, 585
BlsI GCNGC 2 cut(s) 248, 586
Bme1390I CCNGG 3 cut(s) 160, 327, 443
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 2 cut(s) 345, 532
BmrFI CCNGG 3 cut(s) 160, 327, 443
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 1 cut(s) 525
Bpu10I CCTNAGC 1 cut(s) 319
BpuMI CCSGG 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 325, 442, 564
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 441
Bse1I ACTGG 2 cut(s) 37, 235
Bse3DI GCAATG 1 cut(s) 479
BseBI CCWGG 2 cut(s) 160, 327
BseDI CCNNGG 3 cut(s) 325, 442, 564
BseGI GGATG 3 cut(s) 187, 453, 627
BseLI CCNNNNNNNGG 2 cut(s) 283, 441
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 4 cut(s) 231, 279, 473, 593
BseNI ACTGG 2 cut(s) 37, 235
BseXI GCAGC 1 cut(s) 596
BseYI CCCAGC 1 cut(s) 626
BshFI GGCC 1 cut(s) 347
BsiSI CCGG 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 283, 441
BsmAI GTCTC 2 cut(s) 122, 367
BsnI GGCC 1 cut(s) 347
Bso31I GGTCTC 1 cut(s) 122
BspACI CCGC 2 cut(s) 246, 330
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 4 cut(s) 230, 278, 472, 592
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 479
BsrI ACTGG 2 cut(s) 37, 235
BssECI CCNNGG 3 cut(s) 325, 442, 564
BssT1I CCWWGG 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 160, 327
Bst4CI ACNGT 1 cut(s) 206
BstDEI CTNAG 5 cut(s) 217, 265, 319, 459, 579
BstF5I GGATG 3 cut(s) 187, 453, 627
BstMAI GTCTC 2 cut(s) 122, 367
BstMWI GCNNNNNNNGC 2 cut(s) 593, 632
BstNI CCWGG 2 cut(s) 160, 327
BstNSI RCATGY 2 cut(s) 304, 556
BstSCI CCNGG 3 cut(s) 158, 325, 441
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 1 cut(s) 319
BsuRI GGCC 1 cut(s) 347
BtsCI GGATG 3 cut(s) 187, 453, 627
Cfr13I GGNCC 2 cut(s) 345, 532
CseI GACGC 1 cut(s) 485
Csp6I GTAC 5 cut(s) 8, 130, 439, 556, 603
CviAII CATG 4 cut(s) 283, 301, 396, 553
CviJI RGCY 9 cut(s) 13, 96, 249, 323, 341, 347, 587, 609, 626
CviKI_1 RGCY 9 cut(s) 13, 96, 249, 323, 341, 347, 587, 609, 626
CviQI GTAC 5 cut(s) 8, 130, 439, 556, 603
DdeI CTNAG 5 cut(s) 217, 265, 319, 459, 579
DraI TTTAAA 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 297
EciI GGCGGA 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 564
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 532
EcoRII CCWGG 2 cut(s) 158, 325
EcoT14I CCWWGG 1 cut(s) 564
EcoT22I ATGCAT 1 cut(s) 637
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 4 cut(s) 286, 304, 399, 556
FatI CATG 4 cut(s) 282, 300, 395, 552
FauNDI CATATG 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 247, 585
FokI GGATG 3 cut(s) 194, 460, 634
Fsp4HI GCNGC 2 cut(s) 247, 585
GluI GCNGC 2 cut(s) 247, 585
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 442
HgaI GACGC 1 cut(s) 485
Hin1II CATG 4 cut(s) 286, 304, 399, 556
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 2 cut(s) 272, 392
HpaII CCGG 1 cut(s) 442
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 220, 384, 462
Hpy188III TCNNGA 2 cut(s) 292, 619
Hpy8I GTNNAC 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 5 cut(s) 28, 300, 389, 524, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 593, 632
HpyF3I CTNAG 5 cut(s) 217, 265, 319, 459, 579
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 4 cut(s) 286, 304, 399, 556
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 1 cut(s) 596
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 4 cut(s) 145, 289, 373, 473
MboII GAAGA 2 cut(s) 251, 324
MluCI AATT 2 cut(s) 122, 137
MnlI CCTC 1 cut(s) 545
Mph1103I ATGCAT 1 cut(s) 637
MseI TTAA 4 cut(s) 39, 54, 92, 141
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 1 cut(s) 442
MspR9I CCNGG 3 cut(s) 160, 327, 443
MvaI CCWGG 2 cut(s) 160, 327
MwoI GCNNNNNNNGC 2 cut(s) 593, 632
NciI CCSGG 1 cut(s) 443
NdeI CATATG 1 cut(s) 24
NlaIII CATG 4 cut(s) 286, 304, 399, 556
NmuCI GTSAC 2 cut(s) 289, 473
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 2 cut(s) 304, 556
PciI ACATGT 1 cut(s) 552
PfeI GAWTC 2 cut(s) 272, 392
PkrI GCNGC 2 cut(s) 248, 586
PscI ACATGT 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 158, 325
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 2 cut(s) 158, 325
PspPI GGNCC 2 cut(s) 345, 532
RsaI GTAC 5 cut(s) 9, 131, 440, 557, 604
RsaNI GTAC 5 cut(s) 8, 130, 439, 556, 603
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 4 cut(s) 39, 54, 92, 141
SatI GCNGC 2 cut(s) 247, 585
Sau96I GGNCC 2 cut(s) 345, 532
ScrFI CCNGG 3 cut(s) 160, 327, 443
SetI ASST 7 cut(s) 15, 98, 152, 343, 543, 589, 611
SinI GGWCC 1 cut(s) 532
SmiMI CAYNNNNRTG 1 cut(s) 620
Sse9I AATT 2 cut(s) 122, 137
SsiI CCGC 2 cut(s) 246, 330
StyD4I CCNGG 3 cut(s) 158, 325, 441
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 1 cut(s) 543
TaqI TCGA 1 cut(s) 337
TasI AATT 2 cut(s) 122, 137
TatI WGTACW 2 cut(s) 7, 555
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 2 cut(s) 272, 392
Tru1I TTAA 4 cut(s) 39, 54, 92, 141
Tru9I TTAA 4 cut(s) 39, 54, 92, 141
TseFI GTSAC 2 cut(s) 289, 473
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 2 cut(s) 289, 473
TspDTI ATGAA 2 cut(s) 198, 210
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 1 cut(s) 122
XceI RCATGY 2 cut(s) 304, 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.