Rroxscaffold_5G00376380

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010463
Physical Location & Seq
Forward (+)
57089111 .. 57093763
4653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376380.1

Sequence Viewer

Length: 633 bp
ATGATCTCTTCCTTCTGCTACTACCCTTCCCACATCATTGAAAAACTCGACGAAGAGAACCACCGCGAGTTGAAACCTATAACTGCAAGGCACCGGCTTACAGCATCTGATTCAGGAGATGGGAGAAGGAAAAGGCTCGACTCTGGTGCACCTTCTGGTCGTCGTTCTCAGCTTAGTGGCTTTTGGGTTCGCCATTGCCGCCGAGAGACGAAGAAGTGTGTGACTGCTGACTGTGGTGTAATATATAAGGATGAACGAACAAATGAAACTTACTGTGTCTATAACTCAGATGTTGCTACTGGTTATGGAGTGGGCGGCTTCTTGTTTCTTCTCTCGGGGGAATCCCTACTCATGGGTGTCACGAAGGTAACTTTTCTGATTGCAGAATCATGCTTGATTGCTGGTGCTACAAAAAATGCCTATCACACCAAGTACCGGGGGATGATATATGCTCAGAACTTCTCCTGTGACGCACTGCGAAAAGGCGTTTTTATTGCTGGAGCAGTATTTGTGGTTGCAACAATGGTCCTCAACGTCTATTACTACATGTACTTCACCAAGGCAATCACTACTCAGGCAGCTCACAAAGCGAAGCGTACAAGCTCAAACATTGGGATGGCTGGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

210

Amino Acids

23.39

Weight (kDa)

9.37

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 118 - 175 3.9e-13 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 90
AccII CGCG 1 cut(s) 66
AciI CCGC 3 cut(s) 64, 199, 315
AfaI GTAC 3 cut(s) 434, 551, 598
AfiI CCNNNNNNNGG 2 cut(s) 352, 435
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 2 cut(s) 41, 73
AluBI AGCT 3 cut(s) 172, 581, 603
AluI AGCT 3 cut(s) 172, 581, 603
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 1 cut(s) 200
Alw44I GTGCAC 1 cut(s) 147
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 334
ApaLI GTGCAC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 578
AspS9I GGNCC 1 cut(s) 526
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 1 cut(s) 547
AvaI CYCGRG 1 cut(s) 334
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 1 cut(s) 590
BccI CCATC 2 cut(s) 113, 610
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 200
BisI GCNGC 3 cut(s) 199, 316, 579
BlsI GCNGC 3 cut(s) 200, 317, 580
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 1 cut(s) 113
BpmI CTGGAG 1 cut(s) 519
BpuMI CCSGG 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 436, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 435
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 436, 558
BseGI GGATG 3 cut(s) 256, 447, 621
BseLI CCNNNNNNNGG 2 cut(s) 352, 435
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 4 cut(s) 182, 300, 467, 587
BseNI ACTGG 1 cut(s) 304
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 1 cut(s) 590
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 66
BshNI GGYRCC 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 151
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 2 cut(s) 94, 436
BslI CCNNNNNNNGG 2 cut(s) 352, 435
BsmAI GTCTC 1 cut(s) 200
BsmBI CGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 3 cut(s) 64, 199, 315
BspCNI CTCAG 4 cut(s) 181, 299, 466, 586
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 436, 558
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 558
Bst4CI ACNGT 2 cut(s) 233, 275
Bst6I CTCTTC 2 cut(s) 13, 48
BstDEI CTNAG 5 cut(s) 168, 173, 286, 453, 573
BstF5I GGATG 3 cut(s) 256, 447, 621
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 3 cut(s) 198, 587, 626
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 151
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 590
BtsCI GGATG 3 cut(s) 256, 447, 621
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 473
CaiI CAGNNNCTG 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 526
CseI GACGC 1 cut(s) 479
Csp6I GTAC 3 cut(s) 433, 550, 597
CviAII CATG 3 cut(s) 352, 390, 547
CviJI RGCY 8 cut(s) 97, 136, 172, 180, 318, 581, 603, 620
CviKI_1 RGCY 8 cut(s) 97, 136, 172, 180, 318, 581, 603, 620
CviQI GTAC 3 cut(s) 433, 550, 597
DdeI CTNAG 5 cut(s) 168, 173, 286, 453, 573
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 13, 48
EarI CTCTTC 2 cut(s) 13, 48
Eco130I CCWWGG 1 cut(s) 558
Eco47I GGWCC 1 cut(s) 526
Eco88I CYCGRG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 3 cut(s) 355, 393, 550
FatI CATG 3 cut(s) 351, 389, 546
Fnu4HI GCNGC 3 cut(s) 199, 316, 579
FokI GGATG 3 cut(s) 263, 454, 628
Fsp4HI GCNGC 3 cut(s) 199, 316, 579
GluI GCNGC 3 cut(s) 199, 316, 579
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 519
HapII CCGG 2 cut(s) 94, 436
HgaI GACGC 1 cut(s) 479
Hin1II CATG 3 cut(s) 355, 393, 550
HinfI GANTC 4 cut(s) 110, 140, 341, 386
HpaII CCGG 2 cut(s) 94, 436
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 4 cut(s) 109, 289, 378, 456
Hpy188III TCNNGA 2 cut(s) 114, 361
Hpy8I GTNNAC 1 cut(s) 149
Hpy99I CGWCG 2 cut(s) 53, 165
HpyAV CCTTC 5 cut(s) 22, 36, 120, 162, 358
HpyCH4III ACNGT 2 cut(s) 233, 275
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 5 cut(s) 86, 149, 383, 518, 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 587, 626
HpyF3I CTNAG 5 cut(s) 168, 173, 286, 453, 573
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 3 cut(s) 355, 393, 550
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 500
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 1 cut(s) 113
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 4 cut(s) 220, 358, 367, 467
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 3 cut(s) 65, 223, 320
MhlI GDGCHC 1 cut(s) 151
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 1 cut(s) 539
Mph1103I ATGCAT 1 cut(s) 631
MslI CAYNNNNRTG 1 cut(s) 614
MspI CCGG 2 cut(s) 94, 436
MspR9I CCNGG 1 cut(s) 437
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 198, 587, 626
NciI CCSGG 1 cut(s) 437
NdeII GATC 1 cut(s) 3
NlaIII CATG 3 cut(s) 355, 393, 550
NlaIV GGNNCC 1 cut(s) 92
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 3 cut(s) 220, 358, 467
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 1 cut(s) 550
PciI ACATGT 1 cut(s) 546
PfeI GAWTC 3 cut(s) 110, 341, 386
PkrI GCNGC 3 cut(s) 200, 317, 580
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PscI ACATGT 1 cut(s) 546
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 107
RsaI GTAC 3 cut(s) 434, 551, 598
RsaNI GTAC 3 cut(s) 433, 550, 597
RseI CAYNNNNRTG 1 cut(s) 614
SatI GCNGC 3 cut(s) 199, 316, 579
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 1 cut(s) 526
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 1 cut(s) 151
SetI ASST 7 cut(s) 79, 154, 174, 369, 537, 583, 605
SfaNI GCATC 1 cut(s) 113
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 614
SsiI CCGC 3 cut(s) 64, 199, 315
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 2 cut(s) 233, 275
TaiI ACGT 1 cut(s) 537
TaqI TCGA 2 cut(s) 48, 138
TatI WGTACW 1 cut(s) 549
TauI GCSGC 2 cut(s) 201, 318
TfiI GAWTC 3 cut(s) 110, 341, 386
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 3 cut(s) 220, 358, 467
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 3 cut(s) 220, 358, 467
TspDTI ATGAA 2 cut(s) 267, 279
TspRI CASTG 1 cut(s) 480
VneI GTGCAC 1 cut(s) 147
VpaK11BI GGWCC 1 cut(s) 526
XceI RCATGY 1 cut(s) 550
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.