FvH4_4g27350

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010463
Physical Location & Seq
Forward (+)
28384353 .. 28387903
3551 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27350.t1

Sequence Viewer

Length: 576 bp
ATGGGAGAAGGAAAGGGCTCGACTCTGGTGCACCTTCTGGTCGTCGTTCTCAGCTTAGTCGCTTTTGGGTTCGCCATTGCCGCCGAGAGACGAAGAAGTGTGGGTGAAATACATAAGGATGAACAAACAAATCAAACTTACTGTGTCTACAACTCAGATGTTGCCACTGGTTATGGAGTGGGTGGGTTCTTGTTTCTTCTGTCAGGGGAATCACTACTCATGGGTGTCACGAAGTGCATGTGTTTTGGAAGACCCTTAGCCCCTGGTGGAAATCGAGCTTGGAGCATTATATATTTTGTTTCATCATTGGTAACTTTTTTGATTGCAGAATCATGCTTGATTGCTGGTGCTACAAAAAATGCCTATCACACCAAGTACCGGGAAATGATATATGCTCAGAACTTCTCCTGTGATGCATTGCGGAAAGGTGTTTTTATTGCTGGAGCAGTGTTTGTGGTTGCAACAATGGTCCTCAATGTCTATTACTACATCTACTTCACCAAGGCAACAGCTACTCAGGTAGCTCACAAAGCCAAGCGTACAAGCTCAAACATTGGGATGGCTGGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

192

Amino Acids

20.71

Weight (kDa)

9.21

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 63 - 156 1.3e-24 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 147
AciI CCGC 2 cut(s) 81, 421
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 2 cut(s) 377, 541
AfiI CCNNNNNNNGG 1 cut(s) 378
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 5 cut(s) 54, 278, 512, 524, 546
AluI AGCT 5 cut(s) 54, 278, 512, 524, 546
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 82
Alw44I GTGCAC 1 cut(s) 29
ApaLI GTGCAC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 116, 490
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 82
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 2 cut(s) 264, 380
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 264, 380
BmsI GCATC 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 462
Bpu10I CCTNAGC 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 262, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 2 cut(s) 75, 416
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 262, 501
BseGI GGATG 2 cut(s) 124, 564
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMI GCAATG 2 cut(s) 75, 416
BseMII CTCAG 4 cut(s) 64, 168, 410, 530
BseNI ACTGG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 33
BseYI CCCAGC 1 cut(s) 563
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 20, 33
BspACI CCGC 2 cut(s) 81, 421
BspCNI CTCAG 4 cut(s) 63, 167, 409, 529
BsrDI GCAATG 2 cut(s) 75, 416
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 262, 501
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 143
BstDEI CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
BstF5I GGATG 2 cut(s) 124, 564
BstMAI GTCTC 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 80, 530, 569
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 2 cut(s) 262, 378
BstSLI GKGCMC 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 256
BtsCI GGATG 2 cut(s) 124, 564
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 165, 453
Cfr13I GGNCC 1 cut(s) 469
Csp6I GTAC 2 cut(s) 376, 540
CviAII CATG 3 cut(s) 220, 238, 333
CviJI RGCY 9 cut(s) 18, 54, 260, 278, 512, 524, 533, 546, 563
CviKI_1 RGCY 9 cut(s) 18, 54, 260, 278, 512, 524, 533, 546, 563
CviQI GTAC 2 cut(s) 376, 540
DdeI CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
DraIII CACNNNGTG 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 2 cut(s) 418, 574
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 223, 241, 336
FatI CATG 3 cut(s) 219, 237, 332
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 2 cut(s) 131, 571
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 81
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 462
HapII CCGG 1 cut(s) 379
Hin1II CATG 3 cut(s) 223, 241, 336
HinfI GANTC 3 cut(s) 22, 209, 329
HpaII CCGG 1 cut(s) 379
HphI GGTGA 2 cut(s) 116, 490
Hpy166II GTNNAC 2 cut(s) 31, 148
Hpy188I TCNGA 2 cut(s) 157, 399
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 2 cut(s) 31, 148
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 6 cut(s) 31, 237, 326, 416, 461, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 530, 569
HpyF3I CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
Hsp92II CATG 3 cut(s) 223, 241, 336
LmnI GCTCC 2 cut(s) 282, 443
LweI GCATC 1 cut(s) 403
MaeIII GTNAC 2 cut(s) 226, 310
MboII GAAGA 3 cut(s) 105, 188, 261
MhlI GDGCHC 2 cut(s) 20, 33
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 1 cut(s) 482
Mph1103I ATGCAT 2 cut(s) 418, 574
MslI CAYNNNNRTG 2 cut(s) 117, 557
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 264, 380
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 80, 530, 569
NciI CCSGG 1 cut(s) 380
NlaIII CATG 3 cut(s) 223, 241, 336
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 2 cut(s) 418, 574
NspI RCATGY 1 cut(s) 241
PfeI GAWTC 2 cut(s) 209, 329
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 1 cut(s) 262
PspPI GGNCC 1 cut(s) 469
RsaI GTAC 2 cut(s) 377, 541
RsaNI GTAC 2 cut(s) 376, 540
RseI CAYNNNNRTG 2 cut(s) 117, 557
SatI GCNGC 1 cut(s) 81
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 264, 380
SduI GDGCHC 2 cut(s) 20, 33
SetI ASST 8 cut(s) 36, 56, 280, 430, 514, 522, 526, 548
SfaNI GCATC 1 cut(s) 403
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 117, 557
SsiI CCGC 2 cut(s) 81, 421
StyD4I CCNGG 2 cut(s) 262, 378
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 2 cut(s) 20, 274
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 209, 329
TscAI CASTG 2 cut(s) 172, 453
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 135, 291
TspRI CASTG 2 cut(s) 172, 453
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 469
XceI RCATGY 1 cut(s) 241
XmiI GTMKAC 1 cut(s) 147
Zsp2I ATGCAT 2 cut(s) 418, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.