Rh4BG346500

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010463
Physical Location & Seq
Forward (+)
50897226 .. 50901162
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG346500.1

Sequence Viewer

Length: 576 bp
ATGGGAGAAGGAAAAGGCTCGACTCTGGTGCACCTTCTGGTCGTTGTTCTCAGCTTAGTGGCTTTTGGGTTCGCCATTGCCGCCGAGAGACGAAGAAGTGTGGGTGTAATATATAAGGATGAACGAACAAATGAAACTTACTGTGTCTATAACTCAGATGTTGCTACTGGTTATGGAGTGGGCGGCTTCTTGTTTCTTCTCTCGGGGGAATCCCTACTCATGGGTGTCACGAAGTGCATGTGTTTTGGAAGACCCTTAGCCCCTGGCGGAAATCGAGCTTGGACCATTATATATTTTGTCTCATCATTGGTAACTTTTCTGATTGCAGAATCATGCTTGATTGCTGGTGCTACAAAAAATGCCTATCACACCAAGTACCGGGGGATGATATATGCTCAGAACTTCTCCTGTGACGCATTGCGAAAAGGCGTTTTTATTGCTGGAGCAGTATTTGTGGTTGCAACAATGGTCCTCAATGTCTATTACTACATGTACTTCACCAAGGCAATCACTACTCAGGCAGCTCACAAAGCAAAGCGTACAAGCTCAAACATCGGGATGGCTGGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

191

Amino Acids

20.71

Weight (kDa)

9.4

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 63 - 156 1.7e-24 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 81, 183, 267
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 3 cut(s) 377, 494, 541
AfiI CCNNNNNNNGG 2 cut(s) 220, 378
AflIII ACRYGT 1 cut(s) 489
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 4 cut(s) 54, 278, 524, 546
AluI AGCT 4 cut(s) 54, 278, 524, 546
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 82, 304
Alw44I GTGCAC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 202
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 282, 469
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 202
AvaII GGWCC 2 cut(s) 282, 469
BaeGI GKGCMC 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 533
BccI CCATC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 82, 304
BisI GCNGC 3 cut(s) 81, 184, 522
BlsI GCNGC 3 cut(s) 82, 185, 523
Bme1390I CCNGG 2 cut(s) 264, 380
Bme18I GGWCC 2 cut(s) 282, 469
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 2 cut(s) 282, 469
BmrFI CCNGG 2 cut(s) 264, 380
BpiI GAAGAC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 462
Bpu10I CCTNAGC 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 262, 379, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 378
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 2 cut(s) 75, 416
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 262, 379, 501
BseGI GGATG 3 cut(s) 124, 390, 564
BseLI CCNNNNNNNGG 2 cut(s) 220, 378
BseMI GCAATG 2 cut(s) 75, 416
BseMII CTCAG 4 cut(s) 64, 168, 410, 530
BseNI ACTGG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 1 cut(s) 533
BseYI CCCAGC 1 cut(s) 563
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 220, 378
BsmAI GTCTC 2 cut(s) 82, 304
BsmBI CGTCTC 1 cut(s) 82
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 33
BspACI CCGC 3 cut(s) 81, 183, 267
BspCNI CTCAG 4 cut(s) 63, 167, 409, 529
BsrDI GCAATG 2 cut(s) 75, 416
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 262, 379, 501
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 143
BstDEI CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
BstF5I GGATG 3 cut(s) 124, 390, 564
BstMAI GTCTC 2 cut(s) 82, 304
BstMWI GCNNNNNNNGC 3 cut(s) 80, 530, 569
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 2 cut(s) 241, 493
BstSCI CCNGG 2 cut(s) 262, 378
BstSLI GKGCMC 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 256
BtsCI GGATG 3 cut(s) 124, 390, 564
Cfr13I GGNCC 2 cut(s) 282, 469
CseI GACGC 1 cut(s) 422
Csp6I GTAC 3 cut(s) 376, 493, 540
CviAII CATG 4 cut(s) 220, 238, 333, 490
CviJI RGCY 9 cut(s) 18, 54, 62, 186, 260, 278, 524, 546, 563
CviKI_1 RGCY 9 cut(s) 18, 54, 62, 186, 260, 278, 524, 546, 563
CviQI GTAC 3 cut(s) 376, 493, 540
DdeI CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
DraIII CACNNNGTG 1 cut(s) 234
EciI GGCGGA 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 282, 469
Eco88I CYCGRG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 4 cut(s) 223, 241, 336, 493
FatI CATG 4 cut(s) 219, 237, 332, 489
Fnu4HI GCNGC 3 cut(s) 81, 184, 522
FokI GGATG 3 cut(s) 131, 397, 571
Fsp4HI GCNGC 3 cut(s) 81, 184, 522
GluI GCNGC 3 cut(s) 81, 184, 522
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 462
HapII CCGG 1 cut(s) 379
HgaI GACGC 1 cut(s) 422
Hin1II CATG 4 cut(s) 223, 241, 336, 493
HinfI GANTC 3 cut(s) 22, 209, 329
HpaII CCGG 1 cut(s) 379
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 157, 321, 399
Hpy188III TCNNGA 2 cut(s) 229, 556
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 5 cut(s) 31, 237, 326, 461, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 530, 569
HpyF3I CTNAG 6 cut(s) 50, 55, 154, 256, 396, 516
Hsp92II CATG 4 cut(s) 223, 241, 336, 493
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 1 cut(s) 533
MaeIII GTNAC 3 cut(s) 226, 310, 410
MboII GAAGA 3 cut(s) 105, 188, 261
MhlI GDGCHC 1 cut(s) 33
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 1 cut(s) 482
Mph1103I ATGCAT 1 cut(s) 574
MslI CAYNNNNRTG 1 cut(s) 557
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 264, 380
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 80, 530, 569
NciI CCSGG 1 cut(s) 380
NlaIII CATG 4 cut(s) 223, 241, 336, 493
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 2 cut(s) 226, 410
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 2 cut(s) 241, 493
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 2 cut(s) 209, 329
PkrI GCNGC 3 cut(s) 82, 185, 523
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PscI ACATGT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 1 cut(s) 262
PspPI GGNCC 2 cut(s) 282, 469
RsaI GTAC 3 cut(s) 377, 494, 541
RsaNI GTAC 3 cut(s) 376, 493, 540
RseI CAYNNNNRTG 1 cut(s) 557
SatI GCNGC 3 cut(s) 81, 184, 522
Sau96I GGNCC 2 cut(s) 282, 469
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 264, 380
SduI GDGCHC 1 cut(s) 33
SetI ASST 5 cut(s) 36, 56, 280, 526, 548
SinI GGWCC 2 cut(s) 282, 469
SmiMI CAYNNNNRTG 1 cut(s) 557
SsiI CCGC 3 cut(s) 81, 183, 267
StyD4I CCNGG 2 cut(s) 262, 378
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 2 cut(s) 20, 274
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 83, 186
TfiI GAWTC 2 cut(s) 209, 329
TseFI GTSAC 2 cut(s) 226, 410
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 226, 410
TspDTI ATGAA 2 cut(s) 135, 147
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 282, 469
XceI RCATGY 2 cut(s) 241, 493
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.