MD13G1042100.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010463
Physical Location & Seq
Forward (+)
2867499 .. 2871241
3743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1042100.v1.1.491

Sequence Viewer

Length: 786 bp
ATGATCGCGTGGGTCTATGAGGAGTTTCTTTTAGTGAAAAGTGAAAACGGCTTCCATTGCTTTGCAATTCGCAGTGCGTCCAAAGAAAGTTGTCACTTTTTGTTCAGTCTCACTCTTTTTTGTACTCAACTTTCCTACAGAGAGAGAGAGAGAGGGAGACGGGAGAGGAAGGAAGCCATGGGAGAAGGAAAGGGTTCGACTTTGGTGCACCTGCTCGTGGTGGTGCTGAGCTTAGTAGCCTTCGGGTTCGCCATTGCTGCCGAGAGACAAAGAAGTGTGAAGACAAGGGTTTTTAAGGATGTCGTGGAATTGGGCGAAATACGTACAGATGTTGGAACGAATGAAACGTACTGTGTGTATAATTCAGATGTTGCAACTGGTTATGGAGTAGGCGGTTTCTTATTTCTTCTCTCAGGCGAATCACTCCTCATGGGTGTCACGAAATGCATGTGTTTTGGCAGACCCTTGGCCCCTGGTGGAAATCGAGCTTGGTCCATTATATATTTTATTTCATCATGGACAACTTTCCTGGTTGCGGAAGCTTGTCTCATTGCCGGTGCTACAAAAAATGCGTATCACACCAAGTACCGGGAAATGATATATGCTCAGAACTTCTCCTGTGAAGCATTGCGAAAAGGCGTTTTCATTTCTGGAGCAGTATTTGTGGTTGCGACTATGATTCTCAACGTGTATTACTACATGTACTTCACCAAGGCAACTGCTGCTCAGGCAGCTCACAAAGCAAATCGTTCGAGCTCCACTGTCGGGATGACTGGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

262

Amino Acids

29.02

Weight (kDa)

9.06

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 133 - 227 2.4e-25 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 219
AccII CGCG 1 cut(s) 8
AciI CCGC 2 cut(s) 393, 536
AfaI GTAC 5 cut(s) 124, 325, 350, 587, 704
AfiI CCNNNNNNNGG 4 cut(s) 217, 535, 588, 765
AflIII ACRYGT 2 cut(s) 687, 699
AjnI CCWGG 2 cut(s) 472, 528
AjuI GAANNNNNNNTTGG 2 cut(s) 472, 504
AluBI AGCT 5 cut(s) 231, 488, 542, 734, 756
AluI AGCT 5 cut(s) 231, 488, 542, 734, 756
Alw21I GWGCWC 2 cut(s) 210, 758
Alw26I GTCTC 4 cut(s) 113, 151, 259, 551
Alw44I GTGCAC 1 cut(s) 206
AoxI GGCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 206
ApeKI GCWGC 3 cut(s) 257, 722, 731
Asp700I GAANNNNTTC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 469, 492
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 700
AvaII GGWCC 1 cut(s) 492
BaeGI GKGCMC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 758
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 2 cut(s) 210, 758
BbvI GCAGC 3 cut(s) 244, 709, 743
BceAI ACGGC 1 cut(s) 64
BcgI CGANNNNNNTGC 4 cut(s) 187, 221, 732, 766
BciT130I CCWGG 2 cut(s) 474, 530
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 4 cut(s) 113, 151, 259, 551
BfmI CTRYAG 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 219
BisI GCNGC 3 cut(s) 258, 723, 732
BlpI GCTNAGC 1 cut(s) 227
BlsI GCNGC 3 cut(s) 259, 724, 733
Bme1390I CCNGG 3 cut(s) 474, 530, 590
Bme18I GGWCC 1 cut(s) 492
BmgT120I GGNCC 2 cut(s) 469, 492
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 3 cut(s) 474, 530, 590
BmrI ACTGGG 1 cut(s) 783
BmuI ACTGGG 1 cut(s) 783
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 726
Bpu1102I GCTNAGC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 323
BsaJI CCNNGG 4 cut(s) 177, 465, 472, 711
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 535, 588, 765
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 2 cut(s) 382, 778
Bse3DI GCAATG 4 cut(s) 55, 252, 549, 626
BseBI CCWGG 2 cut(s) 474, 530
BseDI CCNNGG 4 cut(s) 177, 465, 472, 711
BseGI GGATG 2 cut(s) 304, 774
BseLI CCNNNNNNNGG 4 cut(s) 217, 535, 588, 765
BseMI GCAATG 4 cut(s) 55, 252, 549, 626
BseMII CTCAG 4 cut(s) 218, 426, 620, 740
BseNI ACTGG 2 cut(s) 382, 778
BseRI GAGGAG 2 cut(s) 35, 416
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 3 cut(s) 244, 709, 743
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 470
BsiHKAI GWGCWC 2 cut(s) 210, 758
BsiSI CCGG 2 cut(s) 555, 589
BslI CCNNNNNNNGG 4 cut(s) 217, 535, 588, 765
BsmAI GTCTC 4 cut(s) 113, 151, 259, 551
BsmBI CGTCTC 1 cut(s) 151
BsnI GGCC 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 210, 758
Bsp143I GATC 1 cut(s) 3
Bsp1720I GCTNAGC 1 cut(s) 227
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 2 cut(s) 393, 536
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 4 cut(s) 219, 425, 619, 739
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 471
BspMI ACCTGC 1 cut(s) 219
BsrDI GCAATG 4 cut(s) 55, 252, 549, 626
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 2 cut(s) 382, 778
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 4 cut(s) 177, 465, 472, 711
BssMI GATC 1 cut(s) 3
BssSI CACGAG 1 cut(s) 215
BssT1I CCWWGG 3 cut(s) 177, 465, 711
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 2 cut(s) 474, 530
Bst4CI ACNGT 2 cut(s) 353, 763
BstAPI GCANNNNNTGC 1 cut(s) 722
BstBAI YACGTR 1 cut(s) 323
BstDEI CTNAG 5 cut(s) 227, 232, 412, 606, 726
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 304, 774
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 4 cut(s) 113, 151, 259, 551
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 6 cut(s) 57, 257, 722, 728, 731, 740
BstNI CCWGG 2 cut(s) 474, 530
BstNSI RCATGY 2 cut(s) 451, 703
BstSCI CCNGG 3 cut(s) 472, 528, 588
BstSFI CTRYAG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 210
BstSNI TACGTA 1 cut(s) 323
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 244, 709, 743
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 2 cut(s) 304, 774
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 759
BveI ACCTGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 2 cut(s) 469, 492
CseI GACGC 1 cut(s) 66
Csp6I GTAC 5 cut(s) 123, 324, 349, 586, 703
CviAII CATG 5 cut(s) 178, 430, 448, 516, 700
CviJI RGCY 9 cut(s) 51, 176, 231, 239, 470, 488, 542, 734, 756
CviKI_1 RGCY 9 cut(s) 51, 176, 231, 239, 470, 488, 542, 734, 756
CviQI GTAC 5 cut(s) 123, 324, 349, 586, 703
DdeI CTNAG 5 cut(s) 227, 232, 412, 606, 726
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 756
Eco105I TACGTA 1 cut(s) 323
Eco130I CCWWGG 3 cut(s) 177, 465, 711
Eco24I GRGCYC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 756
EcoICRI GAGCTC 1 cut(s) 756
EcoRII CCWGG 2 cut(s) 472, 528
EcoT14I CCWWGG 3 cut(s) 177, 465, 711
EcoT22I ATGCAT 2 cut(s) 449, 784
EcoT38I GRGCYC 1 cut(s) 758
ErhI CCWWGG 3 cut(s) 177, 465, 711
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 5 cut(s) 181, 433, 451, 519, 703
FatI CATG 5 cut(s) 177, 429, 447, 515, 699
Fnu4HI GCNGC 3 cut(s) 258, 723, 732
FokI GGATG 2 cut(s) 311, 781
FriOI GRGCYC 1 cut(s) 758
Fsp4HI GCNGC 3 cut(s) 258, 723, 732
GluI GCNGC 3 cut(s) 258, 723, 732
GsuI CTGGAG 1 cut(s) 672
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 555, 589
HgaI GACGC 1 cut(s) 66
Hin1II CATG 5 cut(s) 181, 433, 451, 519, 703
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 2 cut(s) 419, 679
HpaII CCGG 2 cut(s) 555, 589
HphI GGTGA 1 cut(s) 700
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 367, 609
Hpy188III TCNNGA 3 cut(s) 439, 651, 766
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 3 cut(s) 163, 179, 250
HpyCH4III ACNGT 2 cut(s) 353, 763
HpyCH4IV ACGT 3 cut(s) 322, 347, 687
HpyCH4V TGCA 5 cut(s) 65, 208, 374, 447, 782
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 257, 722, 728, 731, 740
HpyF3I CTNAG 5 cut(s) 227, 232, 412, 606, 726
HpySE526I ACGT 3 cut(s) 322, 347, 687
Hsp92II CATG 5 cut(s) 181, 433, 451, 519, 703
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 2 cut(s) 653, 761
Lsp1109I GCAGC 3 cut(s) 244, 709, 743
MaeII ACGT 3 cut(s) 322, 347, 687
MaeIII GTNAC 2 cut(s) 92, 436
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 2 cut(s) 292, 398
MhlI GDGCHC 2 cut(s) 210, 758
MluCI AATT 3 cut(s) 66, 308, 361
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 4 cut(s) 13, 146, 159, 437
Mph1103I ATGCAT 2 cut(s) 449, 784
MroXI GAANNNNTTC 1 cut(s) 193
MseI TTAA 1 cut(s) 294
MspI CCGG 2 cut(s) 555, 589
MspR9I CCNGG 3 cut(s) 474, 530, 590
MvaI CCWGG 2 cut(s) 474, 530
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 6 cut(s) 57, 257, 722, 728, 731, 740
NciI CCSGG 1 cut(s) 590
NcoI CCATGG 1 cut(s) 177
NdeII GATC 1 cut(s) 3
NlaIII CATG 5 cut(s) 181, 433, 451, 519, 703
NlaIV GGNNCC 1 cut(s) 471
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 92, 436
NsiI ATGCAT 2 cut(s) 449, 784
NspI RCATGY 2 cut(s) 451, 703
PaqCI CACCTGC 1 cut(s) 219
PciI ACATGT 1 cut(s) 699
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PdmI GAANNNNTTC 1 cut(s) 193
PfeI GAWTC 2 cut(s) 419, 679
PkrI GCNGC 3 cut(s) 259, 724, 733
Ppu21I YACGTR 1 cut(s) 323
PscI ACATGT 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 758
Psp6I CCWGG 2 cut(s) 472, 528
PspGI CCWGG 2 cut(s) 472, 528
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 2 cut(s) 469, 492
RsaI GTAC 5 cut(s) 124, 325, 350, 587, 704
RsaNI GTAC 5 cut(s) 123, 324, 349, 586, 703
SacI GAGCTC 1 cut(s) 758
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 258, 723, 732
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 2 cut(s) 469, 492
ScrFI CCNGG 3 cut(s) 474, 530, 590
SduI GDGCHC 2 cut(s) 210, 758
SetI ASST 9 cut(s) 213, 233, 325, 350, 490, 544, 690, 736, 758
SfcI CTRYAG 1 cut(s) 136
SinI GGWCC 1 cut(s) 492
SnaBI TACGTA 1 cut(s) 323
Sse9I AATT 3 cut(s) 66, 308, 361
SsiI CCGC 2 cut(s) 393, 536
SstI GAGCTC 1 cut(s) 758
StyD4I CCNGG 3 cut(s) 472, 528, 588
StyI CCWWGG 3 cut(s) 177, 465, 711
TaaI ACNGT 2 cut(s) 353, 763
TaiI ACGT 3 cut(s) 325, 350, 690
TaqI TCGA 3 cut(s) 197, 484, 752
TasI AATT 3 cut(s) 66, 308, 361
TatI WGTACW 2 cut(s) 122, 702
TfiI GAWTC 2 cut(s) 419, 679
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 79, 766
TseFI GTSAC 2 cut(s) 92, 436
TseI GCWGC 3 cut(s) 257, 722, 731
Tsp45I GTSAC 2 cut(s) 92, 436
TspDTI ATGAA 3 cut(s) 357, 501, 634
TspRI CASTG 2 cut(s) 79, 766
VneI GTGCAC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 492
XceI RCATGY 2 cut(s) 451, 703
XmnI GAANNNNTTC 1 cut(s) 193
Zsp2I ATGCAT 2 cut(s) 449, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.