pycom16g03680

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010463
Physical Location & Seq
Forward (+)
2288820 .. 2291179
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03680.1

Sequence Viewer

Length: 576 bp
ATGGGAGAAGGCAAGGGCTCGACTCTGGTGCACCTGCTTGTGGTGGTTCTGAGCTTAGTGGCCTTTGGGTTCGCCATTGCTGCTGAGAGACAAAGAAGTGTGGGCGAAATACGTACAGATATTCAAACAAATCAAACGTACTGTGTGTATAATTCAGATGTCGCAACTGGTTATGGAGTAGGCGGTTTCTTGTTTCTTCTCTCAGGTGAATCACTCCTCATGGGTGTCACGAAGTGCATGTGTTTTGGCAGACCCTTGGCCCCTGGTGGAAATCGAGCTTGGTCCATTATATATTTTGTTTCATCATGGGCAACTTTTCTGGTTGCAGAAGCTTGTCTAATTGCTGGTGCTACAAAAAATGCCTATCACACCAAGTACCGGGGGATGATATATGCTCAGAACTTCTCCTGTGAAGCATTGCGAAAAGGTGTTTTCATTTCTGGAGCAGTATTTGTGGTTGCAACTATGATTCTCAACGTGTATTACTACATGTACTTCACCAAGGCAACTGCTGCTCAGGCAACTCATAAAGCAAATCGTTCGAGCTCAACTGTCGGGATGACTGGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

192

Amino Acids

20.61

Weight (kDa)

9.24

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 63 - 157 1.7e-25 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 42
Acc36I ACCTGC 1 cut(s) 42
AciI CCGC 1 cut(s) 183
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 4 cut(s) 115, 140, 377, 494
AfiI CCNNNNNNNGG 2 cut(s) 40, 378
AflIII ACRYGT 2 cut(s) 477, 489
AgsI TTSAA 1 cut(s) 125
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 4 cut(s) 54, 278, 332, 546
AluI AGCT 4 cut(s) 54, 278, 332, 546
Alw21I GWGCWC 2 cut(s) 33, 548
Alw26I GTCTC 1 cut(s) 82
Alw44I GTGCAC 1 cut(s) 29
AoxI GGCC 2 cut(s) 60, 258
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 2 cut(s) 80, 512
AspS9I GGNCC 2 cut(s) 259, 282
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 218, 490
AvaII GGWCC 1 cut(s) 282
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 2 cut(s) 20, 548
Bbv12I GWGCWC 2 cut(s) 33, 548
BbvI GCAGC 2 cut(s) 67, 499
BcgI CGANNNNNNTGC 4 cut(s) 10, 44, 522, 556
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 82
BfuAI ACCTGC 1 cut(s) 42
BisI GCNGC 2 cut(s) 81, 513
BlsI GCNGC 2 cut(s) 82, 514
Bme1390I CCNGG 2 cut(s) 264, 380
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 2 cut(s) 259, 282
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 2 cut(s) 264, 380
BmrI ACTGGG 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 573
BpmI CTGGAG 1 cut(s) 462
Bpu10I CCTNAGC 1 cut(s) 516
BpuMI CCSGG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 4 cut(s) 255, 262, 379, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 378
Bse1I ACTGG 2 cut(s) 172, 568
Bse3DI GCAATG 2 cut(s) 75, 416
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 255, 262, 379, 501
BseGI GGATG 2 cut(s) 390, 564
BseLI CCNNNNNNNGG 2 cut(s) 40, 378
BseMI GCAATG 2 cut(s) 75, 416
BseMII CTCAG 5 cut(s) 41, 75, 216, 410, 530
BseNI ACTGG 2 cut(s) 172, 568
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 2 cut(s) 67, 499
BshFI GGCC 2 cut(s) 62, 260
BsiHKAI GWGCWC 2 cut(s) 33, 548
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 40, 378
BsmAI GTCTC 1 cut(s) 82
BsnI GGCC 2 cut(s) 62, 260
Bsp1286I GDGCHC 3 cut(s) 20, 33, 548
BspACI CCGC 1 cut(s) 183
BspANI GGCC 2 cut(s) 62, 260
BspCNI CTCAG 5 cut(s) 42, 76, 215, 409, 529
BspLI GGNNCC 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 75, 416
BsrI ACTGG 2 cut(s) 172, 568
BssECI CCNNGG 4 cut(s) 255, 262, 379, 501
BssT1I CCWWGG 2 cut(s) 255, 501
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 143, 553
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 113
BstDEI CTNAG 6 cut(s) 50, 55, 84, 202, 396, 516
BstF5I GGATG 2 cut(s) 390, 564
BstMAI GTCTC 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 80, 512, 518
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 2 cut(s) 241, 493
BstSCI CCNGG 2 cut(s) 262, 378
BstSLI GKGCMC 1 cut(s) 33
BstSNI TACGTA 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 67, 499
BsuRI GGCC 2 cut(s) 62, 260
BtsCI GGATG 2 cut(s) 390, 564
BveI ACCTGC 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 259, 282
Csp6I GTAC 4 cut(s) 114, 139, 376, 493
CviAII CATG 4 cut(s) 220, 238, 306, 490
CviJI RGCY 7 cut(s) 18, 54, 62, 260, 278, 332, 546
CviKI_1 RGCY 7 cut(s) 18, 54, 62, 260, 278, 332, 546
CviQI GTAC 4 cut(s) 114, 139, 376, 493
DdeI CTNAG 6 cut(s) 50, 55, 84, 202, 396, 516
DraIII CACNNNGTG 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 546
Eco105I TACGTA 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 255, 501
Eco24I GRGCYC 2 cut(s) 20, 548
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 255, 501
EcoT22I ATGCAT 1 cut(s) 574
EcoT38I GRGCYC 2 cut(s) 20, 548
ErhI CCWWGG 2 cut(s) 255, 501
FaeI CATG 4 cut(s) 223, 241, 309, 493
FatI CATG 4 cut(s) 219, 237, 305, 489
Fnu4HI GCNGC 2 cut(s) 81, 513
FokI GGATG 2 cut(s) 397, 571
FriOI GRGCYC 2 cut(s) 20, 548
Fsp4HI GCNGC 2 cut(s) 81, 513
GluI GCNGC 2 cut(s) 81, 513
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 2 cut(s) 62, 260
HapII CCGG 1 cut(s) 379
Hin1II CATG 4 cut(s) 223, 241, 309, 493
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 3 cut(s) 22, 209, 469
HpaII CCGG 1 cut(s) 379
HphI GGTGA 2 cut(s) 218, 490
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 51, 157, 399
Hpy188III TCNNGA 3 cut(s) 229, 441, 556
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4III ACNGT 2 cut(s) 143, 553
HpyCH4IV ACGT 3 cut(s) 112, 137, 477
HpyCH4V TGCA 5 cut(s) 31, 237, 326, 461, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 512, 518
HpyF3I CTNAG 6 cut(s) 50, 55, 84, 202, 396, 516
HpySE526I ACGT 3 cut(s) 112, 137, 477
Hsp92II CATG 4 cut(s) 223, 241, 309, 493
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 2 cut(s) 67, 499
MaeII ACGT 3 cut(s) 112, 137, 477
MaeIII GTNAC 1 cut(s) 226
MboII GAAGA 1 cut(s) 188
MhlI GDGCHC 3 cut(s) 20, 33, 548
MluCI AATT 2 cut(s) 151, 339
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 1 cut(s) 227
Mph1103I ATGCAT 1 cut(s) 574
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 264, 380
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 80, 512, 518
NciI CCSGG 1 cut(s) 380
NlaIII CATG 4 cut(s) 223, 241, 309, 493
NlaIV GGNNCC 1 cut(s) 261
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 2 cut(s) 241, 493
PaqCI CACCTGC 1 cut(s) 42
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 2 cut(s) 209, 469
PkrI GCNGC 2 cut(s) 82, 514
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 113
PscI ACATGT 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 2 cut(s) 259, 282
RsaI GTAC 4 cut(s) 115, 140, 377, 494
RsaNI GTAC 4 cut(s) 114, 139, 376, 493
SacI GAGCTC 1 cut(s) 548
SatI GCNGC 2 cut(s) 81, 513
Sau96I GGNCC 2 cut(s) 259, 282
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 264, 380
SduI GDGCHC 3 cut(s) 20, 33, 548
SinI GGWCC 1 cut(s) 282
SnaBI TACGTA 1 cut(s) 113
Sse9I AATT 2 cut(s) 151, 339
SsiI CCGC 1 cut(s) 183
SstI GAGCTC 1 cut(s) 548
StyD4I CCNGG 2 cut(s) 262, 378
StyI CCWWGG 2 cut(s) 255, 501
TaaI ACNGT 2 cut(s) 143, 553
TaiI ACGT 3 cut(s) 115, 140, 480
TaqI TCGA 3 cut(s) 20, 274, 542
TasI AATT 2 cut(s) 151, 339
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 2 cut(s) 209, 469
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 80, 512
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 291, 424
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 282
XceI RCATGY 2 cut(s) 241, 493
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.