AT1G15290

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004812
Physical Location & Seq
Reverse (-)
5256870 .. 5265155
8286 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15290.1

Sequence Viewer

Length: 4827 bp
ATGGCACCGAGGTCAAGCAAAGGGAAATCAAACAACAAAGGGAAAGGAGGAGATAAGAAGAAGAGAGACGATAAACTGTTGGCTCCGAGTCTGGTTGAAATCACAGTCACCACTCCGTACGAAACTCAAGTGATACTCAAGGGAGTTTCTACTGACAAAATCATTGACGTGAGAAGGCTTTTAGCATCTCACGTTGAGACTTGCCATTTCACCAACTATTCTCTCTCCCATAAGGTGAAGGGGCATAAATTGAATGACAACATCCAAGTACTTTCCCTCAAGCCTTGTTTTCTCCGCATGATTCCAGAGGAGTATCTAGAGGAAAGTCAGGCGTTGACTCAGGTACGGAGAGTCATCGACATTGTAGCATGCACCACCAGGTTCTTCTCCAAATCTCCCAACAAATCCATCGTTGCCGGCAATGCCAATCCGACACCGGCGCCGGACGGCCTCGACATGGTCGCTATCCACACCACACCCAAACTTTCTCAGTTCTACGAGTTCTTCTCCATTCACCACCTATCTCCTCCGATTCTTCATTTGAAGAAAGTTGATGGTGAAGAAGCTGGAGAGAAGCGTGATGGTGACTATTTTGGACTTAAGGTTAAGATTTGCAATGGCAAGGTTATACATGTGATTGCTTCTGTAAAAGGGTTCTTCGCCGTTGGAAAACAGCTCTCACATTGTCATTCCATTGTTGATTTGCTCCAGAATGTCAGCAATGCTTTTGCCAAGGCATATGAATCTTTAATGAAAGCTTTCACTGATCGGAATAAGTTTGGCAACCTCCCGTTTGGATTACGCTCAAACACATGGCTTGTACCTTCTCCTGTTTCTGAGTCTGCTTCTCCTTTACCAACAGAGGATGAACATTGGGGCGGGAATGGTGGAGGTCAGGGGAGAAATGGTGAATATGATCATCGACCTTGGGCGGCTGAGTTTTCTGTATTAGCTACGCTTCCTTGCAAGACTGAGGAGGAGAGAGTTATTCGAGATAAGAAAGCTTTTTTGCTTCATAGCCAGTTTATTGATACCTCAGTGCAAAGAGCTGTTAGAGCCATTTGCAACGTGATGGATACTAATCAACAAACAAGTGGGACTACTGACTTACCTGCTGGCTCGATCCTTCTTGAGGATCATGTTGGAGATTTGTCTATTGTTGTAAAACGTGATATAGCCAGCCTTGACTCAAAGCCTGAAGCTACATTTCAAAATGATGCATTTGTTCTATCATCCGAGGAACTTGCTGAAAGGAATTTGCTAAAAGGGATTACAGCTGATGAGAGTGTGATTGTTCATGATACTCCGGCTTTAGGCAAAGTTATTGTAAGACAGTGTGGATATACTGCGGTGGTAAATGTCAAGGGTCAAACTCAGAAGGCAATGTCTGATTTCCGTGATATTTTAATCGATGACCTGCCGGATGGAGGAGCTAATGCTCTTAACCTTAACAGCTTACGGGTAGAGTTTCACAGGCCCCATAGTGTTGGAACATCTGTAGAGAACCAGCCCACACAGCTTGATTGGGATGATTTAGAATCTTATAGATGTATTATACAGGAGTTAGTAAAAATTAACTTAACAAAGTTAGAAGAAACAAGGGTATCTTCTGTAAGACCCATCAGATGGGAACTCGGTTCTACCTGGGTTCAGCATCTACAGAAGAAAGAAACAGACGTCTGTGGAAAGCCTGCAACTAATGATGAAACTGAACTTTCAGTTAAAGGCTTGGGAAAACAATTTAAAGATCTAAAGAGCAAAAGTAAGAAATCAGAAAATATAAGTGCCGTGAATGAAAAAGATACCAGACTGCACGAGTTAAATGAGGAGGATGATCTAGGGCAGAAAAGCATTGATGGACTCTTTACTGAACTGAAGGAACTGCTTTCTGAAGAAGCTTTCTCTCGCCTAAAAGAAACTGGAACTGGTCTCCATTTAAAGTCAAAAGAAGAGCTTACCAATATGGCATATGGTTACTATGATGAAATTGCTTTGCCAAGGCTGGTAGCAGATTTCGGGTCACTGGAGCTTTCTCCAGTTGATGGTCGCACTTTGACTGATTTCATGCATATAAGAGGACTCCAAATGCGTTCTCTAGGACATGTGGCTAAACTTGCTGAGAAACTTCCTCATATACAGTCCCTCTGTATTCATGAGATGATTACAAGAGCTTTCAAGCATCTTCTTAGGGCGGTTATTGCATCTGTTAATAATATGGCAGAGCTACCTGTGGCGGTAGCTGCTTCATTAAATTTTATGCTTGGCCGCCGTGAATTGGAAGGGTGCGATAGAATCCCCGGTGAAGAGTACTGTCTCAGATTACAGTGGCTACAGAAATTTCTCTCCAGAAAATTTGGTTGGATACAGAAAGATGAGTTCCATCATTTAAAAAAGTTTTCTATCCTCCGAGGACTCTGCCAGAAGGTTGGTTTAGAACTAGTTTCAAGAGATTTTGACTTCGATTCTCCAAATCCTTTCATGAGCTCTGACATTATTGGATTGGTACCTGTGTGCAAGCATGTGCTGTGTATTTCATCTGACGGAAGGACACTCTTGGAGTCATCAAAACTTGCTCTTGACAAAGGAAAACTAGATGATGCTGTGAGCTATGGAACAAAGGCATTAGTCAAGATGATTGCTGTCTGTGGGCCTTATCATAGAAATACTGCATGTGCGTACAGTCTTCTCGCAGTTGTACTATACCACACTGGCGATTTTAACCAGGCAACAATATATCAGCAGAAGGCTCTAGATATAAATGAGAGAGAACTTGGTCTCGACCATCCCGACACTATGAAAAGCTATGGGGATCTTTCTGTATTTTACTATCGTCTTCAACATTTTGAATTGGCACTAAAATATGTGAACCGGGCGTTGTTTCTTCTTCACTTCACTTGTGGGCTATCTCACCCAAATACTGCTGCCACATATATCAATGTGGCTATGATGGAAAAAGAAGTAGGGAATGATCATCTAGCTCTAAGGTACCTGCATGAAGCTCTAAAGAGCAATAAAAGATTGCTAGGAGCTGACCATATTCAGACTGCGGCAAGCTATCATGCAATAGCAGTGGCTCTCTCTTTCATGGAAGCACATTCATTGAGTGTGCAACATGAACAAACTACACTTCAGATACTTACTGCAAAACTTGGAGCAGATGACCTTCGTACACAGGATGCTGCTGCATGGCTCGAATATTTTGAATCAAGAGCCATAGAGCAGCAGGAAGCAGGCCGAAATGGAATCCCAAAGCCTGATGCTTCCATCGCGAGCAAAGGCCACCTTAGTGTATCAGATCTCCTGGACTATATAAGTTCTGATCCAGACACTAAGGGAAATGTTGCTCACAGGAAACATCGGCGTGCCAGGATATTACAGGTCAATGATAAGGTTGCATCAGCTGATGATGACGCCCATAGAGTTGCTTCACAGATTGACATTGTTACATGGAACAATGTGGCAGAAGCGGATGTCACTAAGAGTAGGAGCGAAGTAAATGATCCTGATACAGTTGTTGATAAGACCAATATTGAGACAGGTGATATTGTAGTACACAGGCTAAATGTAGACAGGCAAACTGTGGAAGAGTCAACTTTAGACGAGGGCTGGCAAGAGGCATACTCAAAGGGGAGATCCGGAAATGGTGCTGGAAGGAAATCCAGACAAAGGCAGCCTGATCTTATGAAAAAGAGAATGTTGCTTAATAAACATCACAACAGGAATCAGGATGTTCAGCAACAGAATATATATTCTCCCTTGCAGAAAACAAGCAAAGGACCCTCCCTCTCTAAGTCATCACCCCGTAGGGCTCTAAAGAATGCAGAGATAGATGTCAGTACAAATACAACTAAACCACAGTTAAAGGCTTCTGGTGCTGCTGCTGTGACCAGTACTACTTTGGCCTCAAAGTCTCTCTCATACAAAGAGGTTGCTTTGGCACCACCAGGTACAGTTCTGAAGCCAATGTTAGAAAAATTAGAACTGAATTTGGAGAGAACTGAAACTCAGATATACAGGACTTCAAGTGCGTCATCTGGAGAAGAAAGCAAGTCCGACACTGTAATGTTGGATTTGCCAATCGAAGGCACAGAGCTTCACTGTGAGAAACAAGAATCACAAGAAAGTGCTGAGTCAGTGGAAAACTTAACGTCAGAGTCTGAAGGGGATCTGGGGTCCTATCGTGGGAAAAAAACTTCTGATATAAGCCGGACAAAGCTATCAGCCTCCGCAGAGCCTTACAACCCTGGAGGTTTCTTGGTTATTGATCTGCAAAGCTCAGCAGCAACAATAGGCAGCTACCCAATTATGGTTGCAGACCCTATTTCCTGGGCTGTAGTTTCATGTGGGATTCACTCACCACCATATTATAGTGCTATTCACTCAAATGGAGTTGGGACGCCAAGAAGCATGAATCCAGATGCACCAGAGTTTGTTCCGAGAAGATCCTTGCAGAATAGCTCTCAACATGCAGGTGAAGATGCAAGTGTATCCGTCGACTCCAGCAGTTGCCTAAAGGCTGAGAAAGATGCAGTTGATTTGAAGAAGCGAGAGCTTGCCAGCTTCATAGTAAAGTCATCACAGAAAGAGGTTCCGGCTGCTCTTAGCAAAACGAGCCCCGAAGCTGAGTCAGGAGGTACCTCCGAAAAGGATAGTGCAGTAACCGAGATAGTTTATAGTAGAGAAGAGGAGAATGGAGCGAATGCAAATGAGACAAATGGAGGTGAAGGGTTTGTGATTGTGGCAAAGAAAAGGAGAAGGAAGAACAAGGTGCGACTTACAAATGTTGCGGCTGGGCTGTACCATCAACCGTCGTCAGTTTGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1608

Amino Acids

177.63

Weight (kDa)

6.55

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 677 - 757 2.7e-18 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 857 - 921 5e-09 Tetratricopeptide repeat
TPR_NPHP3 PF24885 860 - 959 1.3e-06 Nephrocystin-3 TPR domain
TPR_12 PF13424 889 - 959 1.9e-13 Tetratricopeptide repeat
TPR_10 PF13374 892 - 930 2.4e-08 Tetratricopeptide repeat
TPR_12 PF13424 973 - 1047 7.1e-09 Tetratricopeptide repeat
TPR_10 PF13374 973 - 1013 1.2e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4460
AatII GACGTC 1 cut(s) 1680
Acc36I ACCTGC 4 cut(s) 1120, 1425, 2997, 4460
Acc65I GGTACC 3 cut(s) 2503, 2985, 4634
AccB1I GGYRCC 6 cut(s) 4, 439, 2503, 2985, 3937, 4634
AccB7I CCANNNNNTGG 2 cut(s) 1626, 2042
AccI GTMKAC 2 cut(s) 3567, 4494
AccII CGCG 1 cut(s) 3269
AccIII TCCGGA 1 cut(s) 3635
AclWI GGATC 8 cut(s) 1117, 1143, 2817, 3314, 3494, 3627, 4173, 4437
AcoI YGGCCR 1 cut(s) 2263
AcsI RAATTY 5 cut(s) 1255, 2251, 2336, 2351, 3985
AcuI CTGAAG 6 cut(s) 1218, 1895, 1911, 3113, 3977, 4179
AcyI GRCGYC 4 cut(s) 440, 1677, 3411, 4397
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 2 cut(s) 631, 2101
AhlI ACTAGT 1 cut(s) 2437
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 8 cut(s) 377, 1643, 2721, 3300, 3365, 3943, 4243, 4325
AjuI GAANNNNNNNTTGG 2 cut(s) 2341, 2373
AleI CACNNNNGTG 1 cut(s) 3285
Alw21I GWGCWC 1 cut(s) 2486
Alw26I GTCTC 8 cut(s) 60, 191, 1934, 2318, 2780, 3527, 3915, 4703
AlwI GGATC 8 cut(s) 1117, 1143, 2817, 3314, 3494, 3627, 4173, 4437
AlwNI CAGNNNCTG 1 cut(s) 4157
Aor13HI TCCGGA 1 cut(s) 3635
AoxI GGCC 7 cut(s) 448, 1475, 2263, 2648, 3232, 3277, 3900
ApoI RAATTY 5 cut(s) 1255, 2251, 2336, 2351, 3985
Asp700I GAANNNNTTC 1 cut(s) 758
Asp718I GGTACC 3 cut(s) 2503, 2985, 4634
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 4 cut(s) 1476, 2648, 3776, 4173
AsuC2I CCSGG 2 cut(s) 2298, 2870
AvaII GGWCC 2 cut(s) 3776, 4173
BaeI ACNNNNGTAYC 8 cut(s) 1023, 1056, 1587, 1620, 2495, 2528, 3498, 3531
BanI GGYRCC 6 cut(s) 4, 439, 2503, 2985, 3937, 4634
BanII GRGCYC 3 cut(s) 2486, 3811, 4616
BauI CACGAG 1 cut(s) 1814
BbsI GAAGAC 2 cut(s) 2675, 2825
Bbv12I GWGCWC 1 cut(s) 2486
BceAI ACGGC 4 cut(s) 463, 647, 1772, 2253
BcgI CGANNNNNNTGC 4 cut(s) 1226, 1260, 2397, 2431
BciT130I CCWGG 8 cut(s) 379, 1645, 2723, 3302, 3367, 3945, 4245, 4327
BciVI GTATCC 3 cut(s) 1069, 2355, 4498
BclI TGATCA 2 cut(s) 916, 2968
BcnI CCSGG 2 cut(s) 2298, 2870
BcoDI GTCTC 8 cut(s) 60, 191, 1934, 2318, 2780, 3527, 3915, 4703
BcuI ACTAGT 1 cut(s) 2437
BfaI CTAG 8 cut(s) 317, 1838, 2096, 2438, 2591, 2750, 2975, 3023
BfmI CTRYAG 4 cut(s) 1497, 1658, 2330, 4332
BfoI RGCGCY 1 cut(s) 443
BfrI CTTAAG 1 cut(s) 599
BfuAI ACCTGC 4 cut(s) 1120, 1425, 2997, 4460
BfuI GTATCC 3 cut(s) 1069, 2355, 4498
BglII AGATCT 2 cut(s) 1747, 3295
BlpI GCTNAGC 1 cut(s) 4276
BmcAI AGTACT 3 cut(s) 270, 2309, 3892
Bme18I GGWCC 2 cut(s) 3776, 4173
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 4 cut(s) 1476, 2648, 3776, 4173
BpiI GAAGAC 2 cut(s) 2675, 2825
BplI GAGNNNNNCTC 4 cut(s) 491, 523, 3605, 3637
BpmI CTGGAG 8 cut(s) 588, 692, 2019, 2045, 2329, 4056, 4266, 4483
Bpu1102I GCTNAGC 1 cut(s) 4276
BpuEI CTTGAG 4 cut(s) 111, 122, 263, 1151
BpuMI CCSGG 2 cut(s) 2298, 2870
Bsa29I ATCGAT 1 cut(s) 1410
BsaBI GATNNNNATC 1 cut(s) 1080
BsaHI GRCGYC 4 cut(s) 440, 1677, 3411, 4397
BsaI GGTCTC 2 cut(s) 1934, 2780
BsaWI WCCGGW 1 cut(s) 3635
BsaXI ACNNNNNCTCC 2 cut(s) 892, 922
Bse118I RCCGGY 2 cut(s) 416, 436
Bse1I ACTGG 7 cut(s) 1021, 1924, 1930, 2028, 2036, 2713, 3888
Bse3DI GCAATG 4 cut(s) 427, 622, 727, 1389
Bse8I GATNNNNATC 1 cut(s) 1080
BseAI TCCGGA 1 cut(s) 3635
BseBI CCWGG 8 cut(s) 379, 1645, 2723, 3302, 3367, 3945, 4245, 4327
BseCI ATCGAT 1 cut(s) 1410
BseJI GATNNNNATC 1 cut(s) 1080
BseMI GCAATG 4 cut(s) 427, 622, 727, 1389
BseNI ACTGG 7 cut(s) 1021, 1924, 1930, 2028, 2036, 2713, 3888
BseRI GAGGAG 8 cut(s) 63, 323, 516, 989, 992, 1443, 1841, 4700
BseYI CCCAGC 1 cut(s) 4790
BsgI GTGCAG 2 cut(s) 1796, 4674
Bsh1236I CGCG 1 cut(s) 3269
BshFI GGCC 7 cut(s) 450, 1477, 2265, 2650, 3234, 3279, 3902
BshNI GGYRCC 6 cut(s) 4, 439, 2503, 2985, 3937, 4634
BshVI ATCGAT 1 cut(s) 1410
BsiHKAI GWGCWC 1 cut(s) 2486
BsiWI CGTACG 1 cut(s) 117
BslFI GGGAC 3 cut(s) 1111, 2125, 4408
BsmAI GTCTC 8 cut(s) 60, 191, 1934, 2318, 2780, 3527, 3915, 4703
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 3 cut(s) 1111, 2125, 4408
BsmI GAATGC 2 cut(s) 3823, 4705
BsnI GGCC 7 cut(s) 450, 1477, 2265, 2650, 3234, 3279, 3902
Bso31I GGTCTC 2 cut(s) 1934, 2780
Bsp1286I GDGCHC 3 cut(s) 2486, 3811, 4616
Bsp13I TCCGGA 1 cut(s) 3635
Bsp1720I GCTNAGC 1 cut(s) 4276
Bsp68I TCGCGA 1 cut(s) 3269
BspANI GGCC 7 cut(s) 450, 1477, 2265, 2650, 3234, 3279, 3902
BspDI ATCGAT 1 cut(s) 1410
BspEI TCCGGA 1 cut(s) 3635
BspFNI CGCG 1 cut(s) 3269
BspHI TCATGA 3 cut(s) 1297, 2152, 2478
BspMI ACCTGC 4 cut(s) 1120, 1425, 2997, 4460
BspPI GGATC 8 cut(s) 1117, 1143, 2817, 3314, 3494, 3627, 4173, 4437
BspQI GCTCTTC 1 cut(s) 1944
BspT107I GGYRCC 6 cut(s) 4, 439, 2503, 2985, 3937, 4634
BspTI CTTAAG 1 cut(s) 599
BspTNI GGTCTC 2 cut(s) 1934, 2780
BsrDI GCAATG 4 cut(s) 427, 622, 727, 1389
BsrFI RCCGGY 2 cut(s) 416, 436
BsrI ACTGG 7 cut(s) 1021, 1924, 1930, 2028, 2036, 2713, 3888
BssAI RCCGGY 2 cut(s) 416, 436
BssNI GRCGYC 4 cut(s) 440, 1677, 3411, 4397
BssSI CACGAG 1 cut(s) 1814
BssT1I CCWWGG 3 cut(s) 732, 926, 1997
Bst2BI CACGAG 1 cut(s) 1814
Bst2UI CCWGG 8 cut(s) 379, 1645, 2723, 3302, 3367, 3945, 4245, 4327
Bst6I CTCTTC 5 cut(s) 56, 1944, 2298, 3579, 4677
BstACI GRCGYC 4 cut(s) 440, 1677, 3411, 4397
BstAFI CTTAAG 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstFNI CGCG 1 cut(s) 3269
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 1 cut(s) 442
BstMAI GTCTC 8 cut(s) 60, 191, 1934, 2318, 2780, 3527, 3915, 4703
BstMWI GCNNNNNNNGC 9 cut(s) 422, 1055, 1516, 2114, 2198, 2240, 3266, 3872, 4611
BstNI CCWGG 8 cut(s) 379, 1645, 2723, 3302, 3367, 3945, 4245, 4327
BstNSI RCATGY 6 cut(s) 372, 635, 2105, 2522, 2673, 4469
BstSFI CTRYAG 4 cut(s) 1497, 1658, 2330, 4332
BstUI CGCG 1 cut(s) 3269
BstV2I GAAGAC 2 cut(s) 2675, 2825
BstX2I RGATCY 6 cut(s) 1747, 2809, 3295, 3632, 4165, 4442
BstXI CCANNNNNNTGG 2 cut(s) 1487, 2426
BstYI RGATCY 6 cut(s) 1747, 2809, 3295, 3632, 4165, 4442
Bsu15I ATCGAT 1 cut(s) 1410
BsuI GTATCC 3 cut(s) 1069, 2355, 4498
BsuRI GGCC 7 cut(s) 450, 1477, 2265, 2650, 3234, 3279, 3902
BsuTUI ATCGAT 1 cut(s) 1410
BtgZI GCGATG 1 cut(s) 3250
BtrI CACGTC 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 3075
BtuMI TCGCGA 1 cut(s) 3269
BveI ACCTGC 4 cut(s) 1120, 1425, 2997, 4460
CaiI CAGNNNCTG 1 cut(s) 4157
CciI TCATGA 3 cut(s) 1297, 2152, 2478
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 2 cut(s) 416, 436
Cfr13I GGNCC 4 cut(s) 1476, 2648, 3776, 4173
ClaI ATCGAT 1 cut(s) 1410
CseI GACGC 3 cut(s) 3419, 4017, 4405
CsiI ACCWGGT 2 cut(s) 377, 3943
CspCI CAANNNNNGTGG 4 cut(s) 1501, 1536, 3051, 3086
DinI GGCGCC 1 cut(s) 441
DraI TTTAAA 3 cut(s) 1744, 1938, 2388
EaeI YGGCCR 1 cut(s) 2263
Eam1104I CTCTTC 5 cut(s) 56, 1944, 2298, 3579, 4677
EarI CTCTTC 5 cut(s) 56, 1944, 2298, 3579, 4677
Ecl136II GAGCTC 1 cut(s) 2484
Eco130I CCWWGG 3 cut(s) 732, 926, 1997
Eco24I GRGCYC 3 cut(s) 2486, 3811, 4616
Eco31I GGTCTC 2 cut(s) 1934, 2780
Eco47I GGWCC 2 cut(s) 3776, 4173
Eco53kI GAGCTC 1 cut(s) 2484
Eco57I CTGAAG 6 cut(s) 1218, 1895, 1911, 3113, 3977, 4179
EcoICRI GAGCTC 1 cut(s) 2484
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoO109I RGGNCCY 3 cut(s) 1476, 3776, 4173
EcoRII CCWGG 8 cut(s) 377, 1643, 2721, 3300, 3365, 3943, 4243, 4325
EcoT14I CCWWGG 3 cut(s) 732, 926, 1997
EcoT22I ATGCAT 2 cut(s) 1222, 2070
EcoT38I GRGCYC 3 cut(s) 2486, 3811, 4616
EgeI GGCGCC 1 cut(s) 441
EheI GGCGCC 1 cut(s) 441
ErhI CCWWGG 3 cut(s) 732, 926, 1997
Esp3I CGTCTC 1 cut(s) 60
FalI AAGNNNNNCTT 6 cut(s) 1591, 1623, 1938, 1970, 3267, 3299
FaqI GGGAC 3 cut(s) 1111, 2125, 4408
FauI CCCGC 1 cut(s) 872
FauNDI CATATG 2 cut(s) 739, 1969
FbaI TGATCA 2 cut(s) 916, 2968
FblI GTMKAC 2 cut(s) 3567, 4494
FriOI GRGCYC 3 cut(s) 2486, 3811, 4616
FspBI CTAG 8 cut(s) 317, 1838, 2096, 2438, 2591, 2750, 2975, 3023
GlaI GCGC 1 cut(s) 441
GsaI CCCAGC 1 cut(s) 4794
GsuI CTGGAG 8 cut(s) 588, 692, 2019, 2045, 2329, 4056, 4266, 4483
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 7 cut(s) 450, 1477, 2265, 2650, 3234, 3279, 3902
HgaI GACGC 3 cut(s) 3419, 4017, 4405
HhaI GCGC 1 cut(s) 442
Hin1I GRCGYC 4 cut(s) 440, 1677, 3411, 4397
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 3 cut(s) 336, 3591, 4495
HindII GTYRAC 3 cut(s) 336, 3591, 4495
HindIII AAGCTT 3 cut(s) 756, 1002, 1896
Hpy166II GTNNAC 7 cut(s) 336, 2866, 3170, 3553, 3568, 3591, 4495
Hpy8I GTNNAC 7 cut(s) 336, 2866, 3170, 3553, 3568, 3591, 4495
Hpy99I CGWCG 2 cut(s) 4496, 4813
HpyCH4IV ACGT 6 cut(s) 168, 192, 1068, 1168, 1677, 4148
HpyF10VI GCNNNNNNNGC 9 cut(s) 422, 1055, 1516, 2114, 2198, 2240, 3266, 3872, 4611
HpySE526I ACGT 6 cut(s) 168, 192, 1068, 1168, 1677, 4148
Hsp92I GRCGYC 4 cut(s) 440, 1677, 3411, 4397
HspAI GCGC 1 cut(s) 440
KasI GGCGCC 1 cut(s) 439
Kpn2I TCCGGA 1 cut(s) 3635
KpnI GGTACC 3 cut(s) 2507, 2989, 4638
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Ksp22I TGATCA 2 cut(s) 916, 2968
LguI GCTCTTC 1 cut(s) 1944
LmnI GCTCC 8 cut(s) 88, 711, 1430, 2026, 3026, 3152, 3486, 4694
MabI ACCWGGT 2 cut(s) 377, 3943
MaeI CTAG 8 cut(s) 317, 1838, 2096, 2438, 2591, 2750, 2975, 3023
MaeII ACGT 6 cut(s) 168, 192, 1068, 1168, 1677, 4148
MaeIII GTNAC 8 cut(s) 106, 584, 1973, 2019, 3442, 3472, 3883, 4657
MflI RGATCY 6 cut(s) 1747, 2809, 3295, 3632, 4165, 4442
MhlI GDGCHC 3 cut(s) 2486, 3811, 4616
Mly113I GGCGCC 1 cut(s) 440
MmeI TCCRAC 7 cut(s) 455, 646, 1123, 1468, 2339, 4047, 4077
Mph1103I ATGCAT 2 cut(s) 1222, 2070
MroI TCCGGA 1 cut(s) 3635
MroNI GCCGGC 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 758
MslI CAYNNNNRTG 6 cut(s) 167, 3285, 3360, 4061, 4383, 4470
MspA1I CMGCKG 2 cut(s) 1277, 3401
MspCI CTTAAG 1 cut(s) 599
Mva1269I GAATGC 2 cut(s) 3823, 4705
MvaI CCWGG 8 cut(s) 379, 1645, 2723, 3302, 3367, 3945, 4245, 4327
MvnI CGCG 1 cut(s) 3269
MwoI GCNNNNNNNGC 9 cut(s) 422, 1055, 1516, 2114, 2198, 2240, 3266, 3872, 4611
NaeI GCCGGC 1 cut(s) 418
NarI GGCGCC 1 cut(s) 440
NciI CCSGG 2 cut(s) 2298, 2870
NdeI CATATG 2 cut(s) 739, 1969
NgoMIV GCCGGC 1 cut(s) 416
NmuCI GTSAC 5 cut(s) 106, 584, 2019, 3472, 3883
NruI TCGCGA 1 cut(s) 3269
NsiI ATGCAT 2 cut(s) 1222, 2070
NspI RCATGY 6 cut(s) 372, 635, 2105, 2522, 2673, 4469
OliI CACNNNNGTG 1 cut(s) 3285
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 3 cut(s) 1297, 2152, 2478
PaqCI CACCTGC 1 cut(s) 4460
PciI ACATGT 2 cut(s) 631, 2101
PciSI GCTCTTC 1 cut(s) 1944
PcsI WCGNNNNNNNCGW 1 cut(s) 2784
PctI GAATGC 2 cut(s) 3823, 4705
PdiI GCCGGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 758
Pfl23II CGTACG 1 cut(s) 117
PflFI GACNNNGTC 1 cut(s) 458
PflMI CCANNNNNTGG 2 cut(s) 1626, 2042
PfoI TCCNGGA 1 cut(s) 3300
PluTI GGCGCC 1 cut(s) 443
PpuMI RGGWCCY 2 cut(s) 3776, 4173
PscI ACATGT 2 cut(s) 631, 2101
Psp124BI GAGCTC 1 cut(s) 2486
Psp5II RGGWCCY 2 cut(s) 3776, 4173
Psp6I CCWGG 8 cut(s) 377, 1643, 2721, 3300, 3365, 3943, 4243, 4325
PspFI CCCAGC 1 cut(s) 4790
PspGI CCWGG 8 cut(s) 377, 1643, 2721, 3300, 3365, 3943, 4243, 4325
PspLI CGTACG 1 cut(s) 117
PspPI GGNCC 4 cut(s) 1476, 2648, 3776, 4173
PspPPI RGGWCCY 2 cut(s) 3776, 4173
PstNI CAGNNNCTG 1 cut(s) 4157
PsuI RGATCY 6 cut(s) 1747, 2809, 3295, 3632, 4165, 4442
PsyI GACNNNGTC 1 cut(s) 458
PvuII CAGCTG 2 cut(s) 1277, 3401
RruI TCGCGA 1 cut(s) 3269
RseI CAYNNNNRTG 6 cut(s) 167, 3285, 3360, 4061, 4383, 4470
SacI GAGCTC 1 cut(s) 2486
SalI GTCGAC 1 cut(s) 4493
SapI GCTCTTC 1 cut(s) 1944
Sau96I GGNCC 4 cut(s) 1476, 2648, 3776, 4173
ScaI AGTACT 3 cut(s) 270, 2309, 3892
SduI GDGCHC 3 cut(s) 2486, 3811, 4616
SexAI ACCWGGT 2 cut(s) 377, 3943
SfcI CTRYAG 4 cut(s) 1497, 1658, 2330, 4332
SfoI GGCGCC 1 cut(s) 441
SgrAI CRCCGGYG 1 cut(s) 436
SinI GGWCC 2 cut(s) 3776, 4173
SmiMI CAYNNNNRTG 6 cut(s) 167, 3285, 3360, 4061, 4383, 4470
SmlI CTYRAG 5 cut(s) 126, 137, 278, 599, 1130
SmoI CTYRAG 5 cut(s) 126, 137, 278, 599, 1130
SpeI ACTAGT 1 cut(s) 2437
SphI GCATGC 1 cut(s) 372
SspDI GGCGCC 1 cut(s) 439
SspI AATATT 2 cut(s) 3197, 3529
SspMI CTAG 8 cut(s) 317, 1838, 2096, 2438, 2591, 2750, 2975, 3023
SstI GAGCTC 1 cut(s) 2486
StyI CCWWGG 3 cut(s) 732, 926, 1997
TaiI ACGT 6 cut(s) 171, 195, 1071, 1171, 1680, 4151
TatI WGTACW 6 cut(s) 268, 2307, 2695, 3550, 3836, 3890
TauI GCSGC 4 cut(s) 935, 2268, 3050, 4790
TseFI GTSAC 5 cut(s) 106, 584, 2019, 3472, 3883
Tsp45I GTSAC 5 cut(s) 106, 584, 2019, 3472, 3883
TspGWI ACGGA 5 cut(s) 105, 361, 1385, 2556, 4480
Tth111I GACNNNGTC 1 cut(s) 458
Van91I CCANNNNNTGG 2 cut(s) 1626, 2042
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 3776, 4173
XagI CCTNNNNNAGG 1 cut(s) 1130
XapI RAATTY 5 cut(s) 1255, 2251, 2336, 2351, 3985
XbaI TCTAGA 2 cut(s) 316, 2749
XceI RCATGY 6 cut(s) 372, 635, 2105, 2522, 2673, 4469
XcmI CCANNNNNNNNNTGG 1 cut(s) 3895
XmiI GTMKAC 2 cut(s) 3567, 4494
XmnI GAANNNNTTC 1 cut(s) 758
XspI CTAG 8 cut(s) 317, 1838, 2096, 2438, 2591, 2750, 2975, 3023
ZraI GACGTC 1 cut(s) 1678
ZrmI AGTACT 3 cut(s) 270, 2309, 3892
Zsp2I ATGCAT 2 cut(s) 1222, 2070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.