Rw2G030300

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004812
Physical Location & Seq
Reverse (-)
49587677 .. 49597441
9765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030300.1

Sequence Viewer

Length: 4944 bp
ATGGCCCCAAAATCAGGCAGAGGCAAAAACAACAAGGCCAAGTCTGACAAGAAGAAGAAGGAAGATAAGGTTCCTAGTGTTCTTGACATCACTGTCATTACTCCATATGACACACAAGTTATTCTTAAGGGTATCTCCACTGACAAGATACTTGATGCTAAAAGACTACTGGCTGTGAATGTAGAGACGTGTCATCTTACAAACTATTCCTTGTCTCATGAAGTGAAAGGGAAGAGACTGAATGAAAGGGTGGAGGTGGTTAGTCTGAAACCCTGTCTTCTGAAAATGGTTGAAGAGGACTATAGAGATGAAGCAGAAGCTGTTGCCCACGTGCGGCGTCTGCTGGACCTGGTAGCCTGCACGACCCGATTCGCCAAGCCCAAACGGTCCGCTGCCGCCCCGGACTCCAAGTCCAAGAAGAGCGGCACCAAACCCGTCAACAAGAACTCCGGCCCATCCTCACCGTCCGTTTCGGCTGTCTCGGAGAGCCTCGGGATGGTCGCCATTCACCCGACCCCCAAGCTCTCCGACTTCTACGACTTCTTCTCTTTCTCACACCTCACCCCTCCCATTCTCCATTTACGGAGGTGTGACGTGGACGACGCACAGGAGAGGCGCGACGGTGACTATTTTCACATGCAGATTAAGATTTGCAATGGGAAGCAAGTACAAGTGGTTGCATCAGTAAAAGGGTTCCACACAGTGGGAAAGCAGTTTTTACAAAGCCACTCATTGGTGGATCTTCTGCAACAGCTCAGTAGAGCTTTTGCTAATGCATATGAATCCTTGATGAAAGCTTTTGTAGAGCATAACAAGTTTGGTGACCTTCCATATGGTTTTCGAGCAAACACATGGCTTGTTCCTCCATCTGTTGCCGAGTCTCCATCAATTTTTCCATCTCTGCCGACTGAAGATGAAAATTGGGGAGGAAATGGTGGAGGCCAGGGAAGAAATGGTGAATATGATCGTCGACCATGGGCTACTGACTTTGCAATTTTGGCTTGCCTGCCTTGCAAAACCGAGGAGGAGAGGGTTGTTCGAGACCGAAAAGCATTTTTGCTTCATAGTAAATTCATTGATGTATCGGTCTTTAAAGCTGCTGCTGCAATACGTGGTATAATAAATTCTACTGTGAATGGAAAGGAGACAACCAATTGTTCTCCGGGCTCTGTTCTTTATGAGGAGCGTGTTGGAGACTTGTCCATTGTTGTAAAACATGATATAACAGATCCATTGTCAAAGTCCGAAGTAATAGTTAGTGGTGATCATCCATGCAGTACCCGGGAAGTTGCTCAGAGGTGTTTACTCAAGGGTTTAACTTCTGATGAGAGTGTGGTTGTTCATGATACACCCTCCTTGGGTGTGGTCAATGTAAGGCACTGTGGATACATTGCAACGGTTAAAGTTGTTGGTAAAATAAAGAAGGGAAGCTGTGAGGCTAAAGATATTGACATTGAAGATCAACCAGATGGAGGAGCCAATTCTCTTAATGTGAACAGCCTGAGGGTTCTGCTTCAAAAGTTCAACACTGAATCAGTGGATCACTCTAATTCAGATAGTTTAGAAACTTCTAGGTGTCTGGTTCAAAGAGTATTAAAAGAGAGCTTGGCGAAGTTAGAGGATGAGCCTGCCAATTCTGAGGGGTCTATCAGATGGGAACTTGGTTCTTGCTGGTTGCAACATCTACAAAAGCAGGAAACACCAGTAAACAATTCTGATAGTCCTGAGGATGATATTGAGGCGGCTGAACCAGTTGTTAAAGGTCTAGGGAAGCAATTTAAATTTTTGAAGAAGAGGGAAAAGAAAACAAGTACTGAAGCTGGAACATATGATGAGGAAGACATTGATGCTTATTCTTTGAGTCTAAATGGGGGATCAGATAAATTAGAGGTAAACAATGGCGAGAGTAATGAGATCAGCAATGAGGCAGAATTGAAGACTTTAATTTCAGAAGAAGCCTATTTGCGCCTGAAAGAAGCTGGAACTGGTCTTCATCTAAAGTCAGCAGATGAGCTTATCAAGATGGCGCACAAATATTATGATGAAGTTGCCTTGCCAAAGCTGGTAACAGACTTTGGATCGCTTGAACTCTCTCCAGTTGATGGCCGTACGCTGACAGATTTCATGCATTTAAGGGGGCTGAAAATGCGCTCTTTGGGTCGAGTGGTTGAGCTTTCGGAGAAACTTCCTCATATACAGTCTCTTTGTATTCATGAGATGATTACTCGAGCTTTCAAGCATGTGCTTGAAGCAGTCATTGCTTCTGTTGGCAAAATAACTGACTTGTCTGCGGCAATAGCTGCAACCCTAAATTTTCTACTTGGAGGCTCTGCAATGGATGATGATGTCCTCAAATTGCAATGGCTACGAATATTCCTGGCCCGAAAATTTGGTTGGGCACTGAAAGATGAATTTCAGCACTTGAGGAAGTTATCAATTCTCCGAGGACTTTGCCATAAGGTTGGGCTGGAGTTGGCCCCAAGAGATTATGACATGGAATGCCATAATCCTTTCAGCATATACGACATTATTAGCATGGTTCCAGTGTGTAAGCATGTAGCATGTTCTTCAGCTGATGGACGAAATTTGTTGGAGTCATCTAAAATTGCTCTTGATAAAGGAAAGTTAGAGGATGCTGTACATTTCGGAACAAAGGCACTAGCAAAGATGATAGCTGTCTGTGGCCCCTATCATCGAGTTACTGCAAGTGCTTATAGCCTTTTAGCAGTAGTCCTTTACCACACGGGTGACTTTAATCAGGCAACCATTTATCAGCAGAAGGCTTTAGCTATTAATGAAAGGGAACTTGGGCTGGACCATCCAGATACCATGAAAAGCTATGGGGATCTTTCCGTTTTTTACTACCGTCTTCAATACATCGAATTGGCTTTGAAGTATGTAAATCGTGCGTTGTTTCTTCTTCATTTTACATGTGGACTATCTCACCCTAACACAGCTGCGACATACATAAATGTGGCTATGATGGAGGAGGGCATGGGAAATGTCCATGTTGCTCTCAGATACCTACATGAAGCTTTGAAGTGTAACCAGAGACTATTAGGAGCAGATCACATACAGACTGCTGCAAGCTATCATGCCATAGCCATAGCTCTTTCATTGATGGAAGCATATTCTCTAAGTGTGCAACATGAGCAAACAACACTTAGGATACTTCAAGACAAACTTGGACCTGAGGATCTTCGTACTCAGGATGCAGCAGCATGGCTTGAATATTTTGAATCTAAATCTCTAGAGCAGCAAGAAGCAGCACGAAATGGATCTCCAAAGCCTGATGCCTTGATTGCAAGCAAAGGTCACCTTAGTGTGTCAGATCTCCTTGACTACATTAGCCCTGATCAAGATTCAAAAGTAAATGATGCACATCGGAAGCAGCGACGTGCAAAGGTTTATCAGTCTAGTGATACCATCTATGAGGCACATCAAGATGTAATAGCTGACGATGACTTGCCAAGTGATGGCCTTGAAAACCCTATGATTTTGATAGATAATAATACTGAAGTAGTTGAGGAAAGATGGGTTCATCAGGGACTGGAGGAAAAAGATGATGTCTCTAGAAATGGTTTGTCAGTTGCAAGTGTAACTGCCGAAGAAACAACTTCAGATGAAGGATGGCAAGAAGCCAATTCAAAGGGGAGGTCTGGGAATACCGCTACTGGGCGGAGGTTCAGTAGAAAGAGGCCAGACAGTGAATCCAAATACTCGAGGGAAGTCAAATCCTCATCACAGACTGCAGCTGTGAAGAGCTTTCTAAATGATTCATCTTCATCAAAGCAATCAAAGGTTCGTACTGTGAGTACTGGAGAGGATTCAGTTAGACTCCAGTCCAAAACTACTGTTTCTAAAGTCTTCTCTACTCCAGCCACTCTTACGAACTTGACTTCAAAATCTGTATCTTACAAACAAGTAGCTTTGGCACCTCCAGGTACTGTTCTAAAGTCATTGCTGGATAAAGTTGACGACCTAAATGTGGAAGAATCTGAAACAAAGGAGTGTAACCCCCCACCTGAGACATTGAAAACTGAAGAAAGCATAGGTGTAGTTGAACTCATTCCAAATGATGAGAACGAAGGAACACATGCAAGTTCAACCCAGTTGGAAGAAACAGGCCCTGAAACTCTGGAAGAAAGGTCTGCAGAGAAAAATGGAAGTAAGCTGTCAGCTGCAGCAGAACCATTCAATCCAAGACCACTCACCATGACTCACCCTCTGAATCCAGTTGCTGTTACAAGTGTATATGACGTGAGAGCCAGTCAAGCAATGCTTTCTGCGCCTGTGCTCCCCCCAGTAGCTGCCAGAGTTCCTTGTGGTCCTCGGTCACCTTTGTATTATAAAACCAATTATTCTTTTCGCTTGAGGCAAGGTGTTCAAAAATTTCGAAGTCCCCTTTCAGAAAGAAGTGGATCAGGGCCTCCAAAAATCATGAATCCACATGCTCCAGAGTTTATTCCTGGGAGAACTTTGCAAGCAGATTACATGAATGAATATGCAGAATTTCCAACTGAAGCAAATTCCTCGTTTGAAATGAATAGGGCTGAAGAGACGGATGAAAAATCGAATGGTAAAGCTGAGAGAAAGAGCATCTCTGAGTCTGAGAAGTCAGAGCTTGCAAGGCAGATATTGCTGAGCTTCATAGTAAAATCTGTCCAGCACAACAAGGATTCTGAAAGTGAAAGTAAGCAGGAAAATAATTTAGATGCCGTAGAGAAGGACAGTGCAATTATAAAGATTCACTATGGAAATGAAGGAAAGAAGGACTTAGTTTCTCAATCCAGTGACTATGATCAATCAAAAACAACGGATGTAGATACAAATGAAGTTGGTGATAGTGAAGGATTTACAGTTGTGACAAAGAGGAGAAGAAGCCGGCAGCAGTTCAGAAGTGAGTTGTACAATCAGCAATCCATCTCGGCTTCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1647

Amino Acids

182.3

Weight (kDa)

6.03

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 47 - 118 3.8e-07 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 697 - 779 1.5e-18 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 870 - 932 2.5e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 872 - 971 2.4e-06 Nephrocystin-3 TPR domain
TPR_12 PF13424 901 - 971 6.7e-11 Tetratricopeptide repeat
TPR_10 PF13374 901 - 942 2.5e-10 Tetratricopeptide repeat
TPR_12 PF13424 985 - 1059 3.3e-11 Tetratricopeptide repeat
TPR_10 PF13374 985 - 1025 2.9e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4350, 4742
AasI GACNNNNNNGTC 1 cut(s) 92
AccB1I GGYRCC 2 cut(s) 425, 3934
AccB7I CCANNNNNTGG 4 cut(s) 703, 733, 2102, 3476
AccBSI CCGCTC 1 cut(s) 423
AccI GTMKAC 1 cut(s) 970
AccII CGCG 1 cut(s) 618
AciI CCGC 8 cut(s) 334, 390, 396, 423, 1742, 2291, 3669, 3679
AclWI GGATC 9 cut(s) 747, 1223, 1548, 1882, 2086, 2853, 3204, 3286, 4429
AcoI YGGCCR 1 cut(s) 2104
AcuI CTGAAG 8 cut(s) 930, 1836, 2553, 3537, 3603, 4062, 4542, 4575
AcvI CACGTG 1 cut(s) 331
AcyI GRCGYC 1 cut(s) 337
AdeI CACNNNGTG 1 cut(s) 703
AflII CTTAAG 1 cut(s) 125
AflIII ACRYGT 2 cut(s) 188, 2930
AhdI GACNNNNNGTC 1 cut(s) 409
AjiI CACGTC 4 cut(s) 189, 595, 3398, 4261
AjnI CCWGG 5 cut(s) 348, 942, 2376, 3940, 4468
AjuI GAANNNNNNNTTGG 4 cut(s) 2377, 2409, 2790, 2822
AleI CACNNNNGTG 2 cut(s) 2745, 3321
Alw21I GWGCWC 1 cut(s) 4299
AlwI GGATC 9 cut(s) 747, 1223, 1548, 1882, 2086, 2853, 3204, 3286, 4429
AlwNI CAGNNNCTG 6 cut(s) 320, 3550, 3947, 4130, 4241, 4310
Ama87I CYCGRG 4 cut(s) 491, 1281, 2226, 3721
ArsI GACNNNNNNTTYG 2 cut(s) 3641, 3673
AseI ATTAAT 1 cut(s) 2793
Asp700I GAANNNNTTC 1 cut(s) 4068
AspLEI GCGC 5 cut(s) 618, 1968, 2029, 2151, 4291
AsuC2I CCSGG 4 cut(s) 401, 1164, 1282, 1283
AsuII TTCGAA 1 cut(s) 4396
AvaI CYCGRG 4 cut(s) 491, 1281, 2226, 3721
AvaII GGWCC 5 cut(s) 346, 387, 2815, 3188, 4328
AxyI CCTNAGG 3 cut(s) 1502, 1725, 3192
BaeGI GKGCMC 1 cut(s) 2401
BanI GGYRCC 2 cut(s) 425, 3934
BanII GRGCYC 1 cut(s) 1169
BbrPI CACGTG 1 cut(s) 331
BbsI GAAGAC 6 cut(s) 269, 1845, 1943, 1982, 2861, 3859
Bbv12I GWGCWC 1 cut(s) 4299
BceAI ACGGC 2 cut(s) 2091, 4703
BcgI CGANNNNNNTGC 4 cut(s) 2433, 2467, 4515, 4549
BciT130I CCWGG 5 cut(s) 350, 944, 2378, 3942, 4470
BciVI GTATCC 2 cut(s) 1379, 3162
BclI TGATCA 3 cut(s) 1264, 3355, 4801
BcnI CCSGG 4 cut(s) 401, 1164, 1282, 1283
BfaI CTAG 7 cut(s) 75, 1572, 1766, 2660, 3251, 3417, 3573
BfmI CTRYAG 4 cut(s) 301, 3750, 4152, 4182
BfrI CTTAAG 1 cut(s) 125
BfuI GTATCC 2 cut(s) 1379, 3162
BglII AGATCT 1 cut(s) 3331
BlpI GCTNAGC 1 cut(s) 4643
BmcAI AGTACT 2 cut(s) 1813, 3817
Bme1390I CCNGG 9 cut(s) 350, 401, 944, 1164, 1282, 1283, 2378, 3942, 4470
Bme18I GGWCC 5 cut(s) 346, 387, 2815, 3188, 4328
BmeRI GACNNNNNGTC 1 cut(s) 409
BmeT110I CYCGRG 4 cut(s) 491, 1281, 2226, 3721
BmgBI CACGTC 4 cut(s) 189, 595, 3398, 4261
BmiI GGNNCC 9 cut(s) 6, 72, 427, 695, 1477, 2478, 2541, 2686, 3936
BmrFI CCNGG 9 cut(s) 350, 401, 944, 1164, 1282, 1283, 2378, 3942, 4470
BmrI ACTGGG 3 cut(s) 3684, 4105, 4298
BmsI GCATC 9 cut(s) 145, 689, 1837, 2623, 3202, 3283, 3367, 4608, 4705
BmuI ACTGGG 3 cut(s) 3684, 4105, 4298
BoxI GACNNNNGTC 1 cut(s) 3841
BpiI GAAGAC 6 cut(s) 269, 1845, 1943, 1982, 2861, 3859
BpmI CTGGAG 8 cut(s) 2079, 2489, 3572, 3824, 3840, 3861, 3924, 4440
Bpu1102I GCTNAGC 1 cut(s) 4643
Bpu14I TTCGAA 1 cut(s) 4396
BpuEI CTTGAG 3 cut(s) 1292, 2443, 4393
BpuMI CCSGG 4 cut(s) 401, 1164, 1282, 1283
BsaAI YACGTR 2 cut(s) 331, 1112
BsaBI GATNNNNATC 1 cut(s) 3381
BsaHI GRCGYC 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 1035
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 3673, 3703
Bse118I RCCGGY 1 cut(s) 4884
Bse21I CCTNAGG 3 cut(s) 1502, 1725, 3192
Bse3DI GCAATG 8 cut(s) 661, 1389, 1927, 2256, 2340, 2366, 3959, 4284
Bse8I GATNNNNATC 1 cut(s) 3381
BseBI CCWGG 5 cut(s) 350, 944, 2378, 3942, 4470
BseJI GATNNNNATC 1 cut(s) 3381
BseMI GCAATG 8 cut(s) 661, 1389, 1927, 2256, 2340, 2366, 3959, 4284
BseRI GAGGAG 6 cut(s) 1037, 1040, 1196, 1488, 3002, 4888
BseSI GKGCMC 1 cut(s) 2401
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 618
BshNI GGYRCC 2 cut(s) 425, 3934
BsiHKAI GWGCWC 1 cut(s) 4299
BsiHKCI CYCGRG 4 cut(s) 491, 1281, 2226, 3721
BsiSI CCGG 5 cut(s) 401, 450, 1163, 1282, 4885
BsiWI CGTACG 1 cut(s) 2108
BslFI GGGAC 2 cut(s) 3561, 4386
BsmBI CGTCTC 2 cut(s) 179, 4553
BsmFI GGGAC 2 cut(s) 3561, 4386
BsmI GAATGC 1 cut(s) 2504
Bso31I GGTCTC 1 cut(s) 1035
BsoBI CYCGRG 4 cut(s) 491, 1281, 2226, 3721
Bsp119I TTCGAA 1 cut(s) 4396
Bsp1286I GDGCHC 3 cut(s) 1169, 2401, 4299
Bsp1407I TGTACA 2 cut(s) 2638, 4908
Bsp1720I GCTNAGC 1 cut(s) 4643
Bsp19I CCATGG 1 cut(s) 974
BspACI CCGC 8 cut(s) 334, 390, 396, 423, 1742, 2291, 3669, 3679
BspFNI CGCG 1 cut(s) 618
BspHI TCATGA 4 cut(s) 217, 1342, 2212, 4440
BspLI GGNNCC 9 cut(s) 6, 72, 427, 695, 1477, 2478, 2541, 2686, 3936
BspMAI CTGCAG 3 cut(s) 3754, 4156, 4186
BspPI GGATC 9 cut(s) 747, 1223, 1548, 1882, 2086, 2853, 3204, 3286, 4429
BspQI GCTCTTC 2 cut(s) 413, 3755
BspT104I TTCGAA 1 cut(s) 4396
BspT107I GGYRCC 2 cut(s) 425, 3934
BspTI CTTAAG 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 1035
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 8 cut(s) 661, 1389, 1927, 2256, 2340, 2366, 3959, 4284
BsrFI RCCGGY 1 cut(s) 4884
BsrGI TGTACA 2 cut(s) 2638, 4908
BssAI RCCGGY 1 cut(s) 4884
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 2 cut(s) 974, 1356
Bst2UI CCWGG 5 cut(s) 350, 944, 2378, 3942, 4470
Bst6I CTCTTC 6 cut(s) 227, 288, 413, 1787, 3755, 4551
BstACI GRCGYC 1 cut(s) 337
BstAFI CTTAAG 1 cut(s) 125
BstAPI GCANNNNNTGC 3 cut(s) 2258, 2300, 4639
BstAUI TGTACA 2 cut(s) 2638, 4908
BstBAI YACGTR 2 cut(s) 331, 1112
BstBI TTCGAA 1 cut(s) 4396
BstC8I GCNNGC 9 cut(s) 358, 1003, 1007, 1629, 3088, 3307, 4485, 4626, 4886
BstDSI CCRYGG 1 cut(s) 974
BstEII GGTNACC 3 cut(s) 821, 3314, 4335
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 5 cut(s) 618, 1968, 2029, 2151, 4291
BstNI CCWGG 5 cut(s) 350, 944, 2378, 3942, 4470
BstNSI RCATGY 7 cut(s) 640, 2243, 2558, 2565, 2934, 4100, 4454
BstPAI GACNNNNGTC 1 cut(s) 3841
BstPI GGTNACC 3 cut(s) 821, 3314, 4335
BstSCI CCNGG 9 cut(s) 348, 399, 942, 1162, 1280, 1281, 2376, 3940, 4468
BstSFI CTRYAG 4 cut(s) 301, 3750, 4152, 4182
BstSLI GKGCMC 1 cut(s) 2401
BstUI CGCG 1 cut(s) 618
BstV2I GAAGAC 6 cut(s) 269, 1845, 1943, 1982, 2861, 3859
BstX2I RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
BstXI CCANNNNNNTGG 1 cut(s) 2462
BstYI RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
Bsu36I CCTNAGG 3 cut(s) 1502, 1725, 3192
BsuI GTATCC 2 cut(s) 1379, 3162
BtgI CCRYGG 1 cut(s) 974
BtrI CACGTC 4 cut(s) 189, 595, 3398, 4261
Cac8I GCNNGC 9 cut(s) 358, 1003, 1007, 1629, 3088, 3307, 4485, 4626, 4886
CaiI CAGNNNCTG 6 cut(s) 320, 3550, 3947, 4130, 4241, 4310
CciI TCATGA 4 cut(s) 217, 1342, 2212, 4440
CfoI GCGC 5 cut(s) 618, 1968, 2029, 2151, 4291
Cfr10I RCCGGY 1 cut(s) 4884
Cfr9I CCCGGG 1 cut(s) 1281
CpoI CGGWCCG 1 cut(s) 387
CseI GACGC 2 cut(s) 326, 611
CsiI ACCWGGT 1 cut(s) 348
CspI CGGWCCG 1 cut(s) 387
DraI TTTAAA 2 cut(s) 1093, 1780
DraIII CACNNNGTG 1 cut(s) 703
DrdI GACNNNNNNGTC 1 cut(s) 92
DriI GACNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 2104
Eam1104I CTCTTC 6 cut(s) 227, 288, 413, 1787, 3755, 4551
Eam1105I GACNNNNNGTC 1 cut(s) 409
EarI CTCTTC 6 cut(s) 227, 288, 413, 1787, 3755, 4551
EciI GGCGGA 1 cut(s) 3694
Eco130I CCWWGG 2 cut(s) 974, 1356
Eco24I GRGCYC 1 cut(s) 1169
Eco31I GGTCTC 1 cut(s) 1035
Eco47I GGWCC 5 cut(s) 346, 387, 2815, 3188, 4328
Eco57I CTGAAG 8 cut(s) 930, 1836, 2553, 3537, 3603, 4062, 4542, 4575
Eco72I CACGTG 1 cut(s) 331
Eco81I CCTNAGG 3 cut(s) 1502, 1725, 3192
Eco88I CYCGRG 4 cut(s) 491, 1281, 2226, 3721
Eco91I GGTNACC 3 cut(s) 821, 3314, 4335
EcoO109I RGGNCCY 2 cut(s) 4127, 4427
EcoO65I GGTNACC 3 cut(s) 821, 3314, 4335
EcoRII CCWGG 5 cut(s) 348, 942, 2376, 3940, 4468
EcoT14I CCWWGG 2 cut(s) 974, 1356
EcoT22I ATGCAT 2 cut(s) 778, 2130
EcoT38I GRGCYC 1 cut(s) 1169
ErhI CCWWGG 2 cut(s) 974, 1356
Esp3I CGTCTC 2 cut(s) 179, 4553
FaqI GGGAC 2 cut(s) 3561, 4386
FauNDI CATATG 4 cut(s) 106, 778, 832, 1828
FbaI TGATCA 3 cut(s) 1264, 3355, 4801
FblI GTMKAC 1 cut(s) 970
FriOI GRGCYC 1 cut(s) 1169
FspBI CTAG 7 cut(s) 75, 1572, 1766, 2660, 3251, 3417, 3573
GlaI GCGC 5 cut(s) 617, 1967, 2028, 2150, 4290
GsuI CTGGAG 8 cut(s) 2079, 2489, 3572, 3824, 3840, 3861, 3924, 4440
HapII CCGG 5 cut(s) 401, 450, 1163, 1282, 4885
HgaI GACGC 2 cut(s) 326, 611
HhaI GCGC 5 cut(s) 618, 1968, 2029, 2151, 4291
Hin1I GRCGYC 1 cut(s) 337
Hin6I GCGC 5 cut(s) 616, 1966, 2027, 2149, 4289
HinP1I GCGC 5 cut(s) 616, 1966, 2027, 2149, 4289
HincII GTYRAC 3 cut(s) 439, 971, 3976
HindII GTYRAC 3 cut(s) 439, 971, 3976
HindIII AAGCTT 2 cut(s) 795, 3033
HpaII CCGG 5 cut(s) 401, 450, 1163, 1282, 4885
Hpy166II GTNNAC 9 cut(s) 439, 598, 971, 1304, 1495, 1708, 1894, 2936, 3976
Hpy8I GTNNAC 9 cut(s) 439, 598, 971, 1304, 1495, 1708, 1894, 2936, 3976
Hpy99I CGWCG 4 cut(s) 605, 623, 972, 3399
HpyCH4IV ACGT 6 cut(s) 188, 330, 594, 1111, 3397, 4260
HpySE526I ACGT 6 cut(s) 188, 330, 594, 1111, 3397, 4260
Hsp92I GRCGYC 1 cut(s) 337
HspAI GCGC 5 cut(s) 616, 1966, 2027, 2149, 4289
KroI GCCGGC 1 cut(s) 4884
KroNI GCCGGC 1 cut(s) 4886
Ksp22I TGATCA 3 cut(s) 1264, 3355, 4801
LguI GCTCTTC 2 cut(s) 413, 3755
LmnI GCTCC 5 cut(s) 1183, 1475, 3062, 4302, 4459
LweI GCATC 9 cut(s) 145, 689, 1837, 2623, 3202, 3283, 3367, 4608, 4705
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 7 cut(s) 75, 1572, 1766, 2660, 3251, 3417, 3573
MaeII ACGT 6 cut(s) 188, 330, 594, 1111, 3397, 4260
MbiI CCGCTC 1 cut(s) 423
MfeI CAATTG 1 cut(s) 1153
MflI RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
MhlI GDGCHC 3 cut(s) 1169, 2401, 4299
MlyI GAGTC 7 cut(s) 398, 887, 1870, 2603, 3831, 4213, 4616
MmeI TCCRAC 5 cut(s) 552, 1171, 2571, 4095, 4541
Mph1103I ATGCAT 2 cut(s) 778, 2130
MroNI GCCGGC 1 cut(s) 4884
MroXI GAANNNNTTC 1 cut(s) 4068
MslI CAYNNNNRTG 4 cut(s) 2745, 2972, 3321, 3444
MspA1I CMGCKG 5 cut(s) 392, 2573, 2957, 3755, 4181
MspCI CTTAAG 1 cut(s) 125
MspI CCGG 5 cut(s) 401, 450, 1163, 1282, 4885
MspR9I CCNGG 9 cut(s) 350, 401, 944, 1164, 1282, 1283, 2378, 3942, 4470
MunI CAATTG 1 cut(s) 1153
Mva1269I GAATGC 1 cut(s) 2504
MvaI CCWGG 5 cut(s) 350, 944, 2378, 3942, 4470
MvnI CGCG 1 cut(s) 618
NaeI GCCGGC 1 cut(s) 4886
NciI CCSGG 4 cut(s) 401, 1164, 1282, 1283
NcoI CCATGG 1 cut(s) 974
NdeI CATATG 4 cut(s) 106, 778, 832, 1828
NgoMIV GCCGGC 1 cut(s) 4884
NlaIV GGNNCC 9 cut(s) 6, 72, 427, 695, 1477, 2478, 2541, 2686, 3936
NmeAIII GCCGAG 2 cut(s) 901, 4907
NmuCI GTSAC 8 cut(s) 590, 623, 821, 2747, 3314, 4335, 4793, 4864
NsiI ATGCAT 2 cut(s) 778, 2130
NspI RCATGY 7 cut(s) 640, 2243, 2558, 2565, 2934, 4100, 4454
NspV TTCGAA 1 cut(s) 4396
OliI CACNNNNGTG 2 cut(s) 2745, 3321
PaeR7I CTCGAG 2 cut(s) 2226, 3721
PagI TCATGA 4 cut(s) 217, 1342, 2212, 4440
PciI ACATGT 1 cut(s) 2930
PciSI GCTCTTC 2 cut(s) 413, 3755
PcsI WCGNNNNNNNCGW 1 cut(s) 3391
PctI GAATGC 1 cut(s) 2504
PdiI GCCGGC 1 cut(s) 4886
PdmI GAANNNNTTC 1 cut(s) 4068
Pfl23II CGTACG 1 cut(s) 2108
PflMI CCANNNNNTGG 4 cut(s) 703, 733, 2102, 3476
PleI GAGTC 7 cut(s) 398, 886, 1869, 2602, 3831, 4213, 4615
PmaCI CACGTG 1 cut(s) 331
PmlI CACGTG 1 cut(s) 331
PpsI GAGTC 7 cut(s) 398, 886, 1869, 2602, 3831, 4213, 4615
Ppu21I YACGTR 2 cut(s) 331, 1112
PscI ACATGT 1 cut(s) 2930
PshAI GACNNNNGTC 1 cut(s) 3841
PshBI ATTAAT 1 cut(s) 2793
PsiI TTATAA 2 cut(s) 4350, 4742
Psp6I CCWGG 5 cut(s) 348, 942, 2376, 3940, 4468
PspCI CACGTG 1 cut(s) 331
PspEI GGTNACC 3 cut(s) 821, 3314, 4335
PspGI CCWGG 5 cut(s) 348, 942, 2376, 3940, 4468
PspLI CGTACG 1 cut(s) 2108
PspN4I GGNNCC 9 cut(s) 6, 72, 427, 695, 1477, 2478, 2541, 2686, 3936
PspXI VCTCGAGB 2 cut(s) 2226, 3721
PstI CTGCAG 3 cut(s) 3754, 4156, 4186
PstNI CAGNNNCTG 6 cut(s) 320, 3550, 3947, 4130, 4241, 4310
PsuI RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
PvuII CAGCTG 4 cut(s) 2573, 2957, 3755, 4181
RseI CAYNNNNRTG 4 cut(s) 2745, 2972, 3321, 3444
Rsr2I CGGWCCG 1 cut(s) 387
RsrII CGGWCCG 1 cut(s) 387
SalI GTCGAC 1 cut(s) 969
SapI GCTCTTC 2 cut(s) 413, 3755
ScaI AGTACT 2 cut(s) 1813, 3817
SchI GAGTC 7 cut(s) 398, 887, 1870, 2603, 3831, 4213, 4616
ScrFI CCNGG 9 cut(s) 350, 401, 944, 1164, 1282, 1283, 2378, 3942, 4470
SduI GDGCHC 3 cut(s) 1169, 2401, 4299
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 9 cut(s) 145, 689, 1837, 2623, 3202, 3283, 3367, 4608, 4705
SfcI CTRYAG 4 cut(s) 301, 3750, 4152, 4182
Sfr274I CTCGAG 2 cut(s) 2226, 3721
SfuI TTCGAA 1 cut(s) 4396
SinI GGWCC 5 cut(s) 346, 387, 2815, 3188, 4328
SlaI CTCGAG 2 cut(s) 2226, 3721
SmaI CCCGGG 1 cut(s) 1283
SmiI ATTTAAAT 1 cut(s) 1780
SmiMI CAYNNNNRTG 4 cut(s) 2745, 2972, 3321, 3444
SmlI CTYRAG 6 cut(s) 125, 1307, 2226, 2422, 3721, 4372
SmoI CTYRAG 6 cut(s) 125, 1307, 2226, 2422, 3721, 4372
SsiI CCGC 8 cut(s) 334, 390, 396, 423, 1742, 2291, 3669, 3679
SspI AATATT 3 cut(s) 2036, 2373, 3233
SspMI CTAG 7 cut(s) 75, 1572, 1766, 2660, 3251, 3417, 3573
StyD4I CCNGG 9 cut(s) 348, 399, 942, 1162, 1280, 1281, 2376, 3940, 4468
StyI CCWWGG 2 cut(s) 974, 1356
SwaI ATTTAAAT 1 cut(s) 1780
TaiI ACGT 6 cut(s) 191, 333, 597, 1114, 3400, 4263
TaqII GACCGA 3 cut(s) 1059, 1075, 4323
TatI WGTACW 5 cut(s) 667, 1811, 2638, 3815, 4908
TauI GCSGC 5 cut(s) 337, 398, 426, 1745, 2294
TseFI GTSAC 8 cut(s) 590, 623, 821, 2747, 3314, 4335, 4793, 4864
Tsp45I GTSAC 8 cut(s) 590, 623, 821, 2747, 3314, 4335, 4793, 4864
TspGWI ACGGA 5 cut(s) 457, 598, 2842, 4577, 4832
TspMI CCCGGG 1 cut(s) 1281
Van91I CCANNNNNTGG 4 cut(s) 703, 733, 2102, 3476
Vha464I CTTAAG 1 cut(s) 125
VpaK11BI GGWCC 5 cut(s) 346, 387, 2815, 3188, 4328
VspI ATTAAT 1 cut(s) 2793
XbaI TCTAGA 2 cut(s) 3250, 3572
XceI RCATGY 7 cut(s) 640, 2243, 2558, 2565, 2934, 4100, 4454
XcmI CCANNNNNNNNNTGG 1 cut(s) 950
XhoI CTCGAG 2 cut(s) 2226, 3721
XmaI CCCGGG 1 cut(s) 1281
XmiI GTMKAC 1 cut(s) 970
XmnI GAANNNNTTC 1 cut(s) 4068
XspI CTAG 7 cut(s) 75, 1572, 1766, 2660, 3251, 3417, 3573
ZrmI AGTACT 2 cut(s) 1813, 3817
Zsp2I ATGCAT 2 cut(s) 778, 2130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.