MD17G1215200.v1.1

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004812
Physical Location & Seq
Forward (+)
26215510 .. 26226611
11102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1215200.v1.1.491

Sequence Viewer

Length: 4980 bp
ATGGCACCAAAGTCAGGGAGAGGCAAGAACAGGTCCAAGTCAGACAAGAAGAAGAAAGAAGAGAAGGTTCCCAGTGTTCTTGACATCACTGTCATTACCCCATATGACACACAAGTCATTCTCAAGGGCATCTCCACTGACAAGATACTTGATGTGAGAAAATTACTCGCTGTAAATGTGGAGACATGCCATCTTACAAACTATTCCCTCTCTCATGAAGTCAAGGGGAAAAAATTGAATGAAAAGGTGGAAGTCGTTAGCCTGAAACCTTGTGTTTTAAAAATGGTTGAAGAGGACTACAGCGATGAAGCCCAGGCTGAGGCTCACGTGCGGCGATTGCTGGACCTGGTGGCCTGCACGACCCGATTCGCCAAGCCCAAACGGTCCGCCACTAACCCGGACTCCAAGTCTAAGAAGAACGGCAATAGGCCTCGGACCAGGACATCGGGCCCTCCATCTCCGTCCGATGCGGGCGATGGCCGTCCGACTTCACCGCATTCCGAACCGTCCTTTTCGGCGATCTCGGAGAGCCTAGGGATGGTCGCCATTCATCCGACGCCGAAGCTCTCCGACTTCTATGAGTTCTTCGCTTTCTCTCACCTCTCCCCTCCAATTCTCCATTTGAAGAGATGCAACTTGGAGGATGTACATGAGAGGCGCGATGGTGACTATTTTCAAATTCAGATTAAGATTTGCAATGGGAAGCAAGTACAAGTTGTTGCATCTGTCAAAGGGTTTTACACTGTGGGAAAGCAGTTTTTACAAAGCCACTCGCTCGTGGATCTTCTGCAGCAGTTCAGCAGAGCTTTTGCAAATGCATATGAATCCTTGACGAAGGCTTTTGTAGAACATAACAAGTTTGGCGATCTTCCATATGGTTTCCGAGCAAATACATGGCTTGTCCCTCCATCAGTTGCTGAGTCTCCATCGGAATTTCCACCCCTGCCAACCGAAGATGAAAATTGGGGTGGAAATGGTGGAGGCCAGGGAAGAAATGGCGAATATGATCTTCGACCATGGGCTACAGATTTTGCAATACTGGCTTCCCTTCCTTGCAAAACTGAAGAAGAGAGGGTTATTCGAGACCGGAAGGCATTTTTGCTTCATGGTAGATTTATTGACGTTGCAGTCCTTAAAGCTGCTTCAGCAATACGCGGTGTAATAGACTCCAATATGAATGCAAAGGAGACACTGAAATGTTCTCAAGGCTCCGTTCTTTTCGAGGATCACATTGGAGACTTGTCCATTGTTGTAAAACGTGATACAACAGATGCATGGTCAAACTCTGATTTGTCTACCAAGGAGGCTGCGCAGAGGCTTTTACTTAAGGGGTTAACTTCTGATGAGAGCGTAGTTGTTCGTGATACTTCGTCCTTGGGTGAAGTCAATGTAAGGCACTGTGGATATACTGCAACGATTAAAGTTGTCGGTAACATAAAGAATGGCAACCGCGAGGCTAAAGATATTGATATTGAAGATCAACCAGATGGAGGAGCTAATTCTCTCAATGTTAACAGCTTGAGGGTTCTGCTTCAAAAGTCTAAAACTGAATCATTGGCTCCCAGTTTGAATGGTTTAGAAACTTCTACGTGTCTGGTTCGGAAAGTAATAAAAGAGAGCTTGAAAAATCTAGAAAATGAGCCTGCCAGTTTGGAAAGATCTATCAGATGGGAGCTTGGTTCTTGCTGGGTGCAACATTTACAAAAGCAGGAAACCGCTGCGGTTAACAACTCTGATAGTCCTAAGGCTGATAAAGAGGCTGAACCGATTGTGAAAGGTCTGGGAAAGCAATTTAAACTTTTGAAGAAGAGGGAAAAGAAAACAATTGGAGAAAGCAGAACATATGACGAGGAAGAAATTGATTCTAGTGAAAGCCGAACAGTAGAGCTAGAGAATGGAGATATCGATGATGAGCCAGAATTGAAACAGTTAATTTCAGAAGAAGCCTTTTTGCGCCTCAAAGAAACGGGAACCAATCTTCACCTAAAGTGTGCAGATGAGCTTATCAAGATGGCACACAAATATTATGATGAAATTGCCTTGCCAAAGCTGGTAACAGACTTCGGATCACTTGAACTTTCTCCAGTTGATGGCCGCACATTGACAGACTTCATGCATTTAAGGGGACTGAAGATGCGCTCTTTGGGTTGTGTGGTTGAACTGTCAGAGAATCTTCCTCATATACGGTCACTTTGTATCCATGAAATGGTTACTCGAGCTTTCAAGCACTTGCTTGAAGTAGTCATTTCCCGTGTTGACAAAATAACTGACTTATCTGCGGCAATAGCATCAACCTTAAATTTTCTACTCAGAGGTTCTGTGGTGGAAGATGACGCCCTCAGATTGCAATGGTTACGATTATTTCTATCCAAAAGATTTGGTTGGACACTGAAAGATGAATTTCCGCACTTGAGGAAGTTATCAATTCTCCGAGGACTTTGTCATAAGGTTGGTCTAGAGTTGGCCCCAAAAGATTATGACATGGATTTCCAAAACCCTTTCAGCAAATACGACATTATTAGCATGGTTCCAGTGTGTAAGCATGTAACGTGCTCTTCAGCCGATGGGCGAAATTTGTTGGAGTCATCTAAAATTGCTCTTGATAAAGGAAAGCTAGAGGACGCTGTTAATTTTGGAACAAAGGCACTAGCAAAGATGATAGCTGTTTGCGGTCCTTATCATCGAGTTACAGCAAGTGCTTACAGCCTTGTAGCAGTAGTTCTTTACCACACCGGTGATTTCAATCAGGCGACCATTTATCAGCAGAAGGCTCTGGCTATAAACGAAAGGGAACTTGGGCTTGACCATCCAGATACCATGAAAAGCTATGGGGATCTGTCCGTTTTCTATTACCGTCTTCAATACATCGAATTGGCTTTGAAGTACGTAAATCGTGCTTTGTTTCTTCTTCATTTTACGTGTGGTCTATCTCACCCTAACACAGCTGCAACATACATTAATGTGGCTATGATGGAAGAGGGCATGGGAAATGTCCATGTTGCTCTCAGATACCTGCACGAAGCTCTCAAGTGTAATCAGAGACTGTTAGGAGCAGATCATATACAGACTGCTGCAAGCTATCATGCCATAGCCATAGCTCTCTCATTGATGGAAGCATATTCCCTCAGTGTGCAGCATGAGCAAACAACACTTAAGATACTTCAGGCCAAACTTGGACCGGATGATCTTCGTACTCAGGACGCAGTTGCATGGCTTGAATATTTTGAATCTAAATCTCTGGAGCAGCAAGAAGCAGCCCGAAATGGATTTCCGAAGCCAGATGCGTTAATTGCAAGCAAAGGTCACCTTAGTGTATCAGATCTCCTAGATTTCATTACTCCTGATCAAGATTCGAAAGTAAATGATGCACATAGGAAGCAGCGCCGCACAAAGGTACATCAGTCGAGTGATAATATCCCTCCGGCACACCAAAATGCAATAGCTTATGATGACTTGCCAAATCATGGCGTGGAGAACACTACAATTTTGATAGATGATAAAACTGATGCAGTTGAGGACAGATCCGTTCATCAGGACCTGGAGGAGAAGGGTGATATTTCTACAAAAGGTTTGTCAGTTACGAGCATAACTGTTGAAGAAACAACTTCAGATGAAGGCTGGCAAGAAGCTAATTCGAAGGGTCGGTCTGGAAGTACTGCTACTGGCCGGAAGTTTGGTCGAAGAAGGCCAGAGAGTGAATATTCAAATTTTAGAGAAAGCAAATACCGGAGGGATGTCATTTCTCCACCACGGACTTCAGCTCCTAAGAGTTTTCCGAGTGATTTGTATTCACCAAAGCAGTCAAAGGTTCGTACTCTGAGTGCAGGAGAAGATTCAGTTAAACTTCAGTCCAAAATTTCTGTTTCTAAAGTCCCCTCTATTCCAGCAACTACGAATTTGGCTTCGAAATCTGTATCTTACAAAGAAGTAGCTTTGGCACCTCCAGGTACGGTTCTAAGGTCACTGCACGATAAAGTTGAAAGCTTAAGTGTGGAAAATGCTGAAGCCGAGACATGCAATACTCCCGCTGAGACTTTGAAATTTGAGGAAAGCATAGGTAACTCTGCGGTTGAAGTGATTCCAAATGACGGGGAGGAAGGAACAGGTCTTGCAAGTGCATCCCAATCAGAAGATACAGGCCCTGAAATTGTAGAGGAAAGGTCGGGAGAAAAAAATGGAAGTAAGCTTTCAGCAGCTGCTGAACCTTACAATCCAAGACCACTAACAACTACTCTTCCTCTAAATCCGGTTGCTGTTACAAGTCTATACGATGTGAGAGACAGTCAAGTAATGCTTTCAGCGCCAGTGCTTCCCCCAGTAGCTGCTAGAGTTCCTTGTGGTCCTCGGTCGTCACTGTATTACAAAACCAATTATTCCTTCCGCTTGAGACAAGGTGTTCAGAAATTTCAAAGATCTGTTACAGAAAGAGGGGGTTCTGGTCCTCCAAGAATCATGAACCCGCATGCTCCTGAGTTTGTTCCCGGGAAAACTTGGCATGTGGATACTCTAGACGAATGTGCATATGTTGCAACTGAATCAAATCCTTCGCTTGAAGTTAGTGGGGCTGCAGAGGAGGAACGACAAGAAATTTCCAGTAGCAAAGCTGAAGATGGTATCTTGAGAAAGAGCATCTCCGATTCTGAGAAATCCGAGCTTGCAAGGCAGATGTTGCTCAGCTTCATAGTAAAATCCGTGCAGCAGAACAAGGATCCTGCAAATGAAAGTAAGCAAGGGAATCAATCAGATGCCATTGAAGATGACAGTGCGATAATAAAAATTCACTATGGAGATGAAGGAAAGAGGGACTTGGTTTCTGAATCCGGTGGCTCGGAGCAACCAACAGTAACGGATGTAAAGAGTAAGGAAGGGGGGGACGCTGAAGGATTTACACTTGTGACGAACAGGAGAAGAAGCCGGCAGCTGAGAAGCGGGGTAGCGGGGTTGTACAATCAGCAATCTATCTCGGCTTCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1660

Amino Acids

183.38

Weight (kDa)

6.24

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 46 - 117 8.7e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 693 - 774 5.9e-17 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 866 - 929 1e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 868 - 967 9.6e-06 Nephrocystin-3 TPR domain
TPR_12 PF13424 897 - 967 2.7e-10 Tetratricopeptide repeat
TPR_10 PF13374 897 - 938 3e-09 Tetratricopeptide repeat
TPR_12 PF13424 981 - 1055 5.7e-11 Tetratricopeptide repeat
TPR_10 PF13374 981 - 1021 3e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 89, 113, 1127
Acc16I TGCGCA 1 cut(s) 1311
Acc36I ACCTGC 1 cut(s) 3021
AccB1I GGYRCC 2 cut(s) 4, 3937
AccB7I CCANNNNNTGG 3 cut(s) 2090, 2206, 3464
AccI GTMKAC 1 cut(s) 1295
AccII CGCG 3 cut(s) 660, 1155, 1452
AclWI GGATC 7 cut(s) 789, 1233, 2074, 2841, 3516, 4703, 4716
AcoI YGGCCR 3 cut(s) 478, 2092, 3664
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 2 cut(s) 557, 2334
AfaI GTAC 9 cut(s) 648, 711, 2885, 3193, 3396, 3655, 3814, 3949, 4946
AfiI CCNNNNNNNGG 9 cut(s) 14, 319, 538, 1490, 2090, 2206, 3391, 3464, 4088
AflII CTTAAG 3 cut(s) 1325, 3152, 3985
AflIII ACRYGT 2 cut(s) 1589, 2918
AgeI ACCGGT 1 cut(s) 2732
AhdI GACNNNNNGTC 1 cut(s) 406
AjnI CCWGG 6 cut(s) 312, 345, 437, 984, 3537, 3943
AjuI GAANNNNNNNTTGG 4 cut(s) 2778, 2810, 4361, 4393
AleI CACNNNNGTG 2 cut(s) 2733, 3309
Alw21I GWGCWC 1 cut(s) 2555
AlwI GGATC 7 cut(s) 789, 1233, 2074, 2841, 3516, 4703, 4716
AlwNI CAGNNNCTG 7 cut(s) 917, 3043, 3538, 4142, 4196, 4199, 4322
Ama87I CYCGRG 2 cut(s) 2214, 4481
ApaI GGGCCC 1 cut(s) 452
ArsI GACNNNNNNTTYG 4 cut(s) 3557, 3589, 3629, 3661
AseI ATTAAT 1 cut(s) 2958
AsiGI ACCGGT 1 cut(s) 2732
Asp700I GAANNNNTTC 1 cut(s) 4077
AspA2I CCTAGG 1 cut(s) 532
AspLEI GCGC 6 cut(s) 660, 1312, 1956, 2139, 3384, 4303
AsuC2I CCSGG 3 cut(s) 398, 4482, 4483
AsuII TTCGAA 3 cut(s) 3353, 3635, 3905
AvaI CYCGRG 2 cut(s) 2214, 4481
AvaII GGWCC 9 cut(s) 33, 343, 384, 435, 2672, 3176, 3535, 4340, 4439
AvrII CCTAGG 1 cut(s) 532
AxyI CCTNAGG 1 cut(s) 1743
BaeGI GKGCMC 1 cut(s) 452
BaeI ACNNNNGTAYC 2 cut(s) 3386, 3419
BamHI GGATCC 1 cut(s) 4708
BanI GGYRCC 2 cut(s) 4, 3937
BanII GRGCYC 1 cut(s) 452
BarI GAAGNNNNNNTAC 2 cut(s) 3643, 3675
BauI CACGAG 1 cut(s) 776
BbrPI CACGTG 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 2849
Bbv12I GWGCWC 1 cut(s) 2555
BbvCI CCTCAGC 1 cut(s) 318
BceAI ACGGC 2 cut(s) 436, 465
BciT130I CCWGG 6 cut(s) 314, 347, 439, 986, 3539, 3945
BciVI GTATCC 2 cut(s) 2207, 4495
BclI TGATCA 1 cut(s) 3343
BcnI CCSGG 3 cut(s) 398, 4482, 4483
BfmI CTRYAG 4 cut(s) 298, 788, 1023, 4566
BfoI RGCGCY 2 cut(s) 3385, 4304
BfrI CTTAAG 3 cut(s) 1325, 3152, 3985
BfuAI ACCTGC 1 cut(s) 3021
BfuI GTATCC 2 cut(s) 2207, 4495
BglII AGATCT 3 cut(s) 1658, 3319, 4412
BlnI CCTAGG 1 cut(s) 532
BlpI GCTNAGC 1 cut(s) 4673
BmcAI AGTACT 1 cut(s) 3655
Bme1390I CCNGG 9 cut(s) 314, 347, 398, 439, 986, 3539, 3945, 4482, 4483
Bme18I GGWCC 9 cut(s) 33, 343, 384, 435, 2672, 3176, 3535, 4340, 4439
BmeRI GACNNNNNGTC 1 cut(s) 406
BmeT110I CYCGRG 2 cut(s) 2214, 4481
BmrFI CCNGG 9 cut(s) 314, 347, 398, 439, 986, 3539, 3945, 4482, 4483
BmrI ACTGGG 3 cut(s) 66, 1557, 4310
BmuI ACTGGG 3 cut(s) 66, 1557, 4310
BpiI GAAGAC 1 cut(s) 2849
BpmI CTGGAG 4 cut(s) 2067, 3260, 3560, 3927
Bpu10I CCTNAGC 1 cut(s) 318
Bpu1102I GCTNAGC 1 cut(s) 4673
Bpu14I TTCGAA 3 cut(s) 3353, 3635, 3905
BpuEI CTTGAG 7 cut(s) 107, 1188, 1540, 2431, 3011, 4405, 4639
BpuMI CCSGG 3 cut(s) 398, 4482, 4483
Bsa29I ATCGAT 1 cut(s) 1905
BsaAI YACGTR 4 cut(s) 328, 1590, 2887, 2919
BsaBI GATNNNNATC 1 cut(s) 2742
BsaHI GRCGYC 2 cut(s) 557, 2334
BsaI GGTCTC 1 cut(s) 1077
BsaWI WCCGGW 6 cut(s) 1086, 2732, 3178, 3726, 4246, 4820
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 3745, 3775
Bsc4I CCNNNNNNNGG 9 cut(s) 14, 319, 538, 1490, 2090, 2206, 3391, 3464, 4088
Bse118I RCCGGY 2 cut(s) 2732, 4914
Bse21I CCTNAGG 1 cut(s) 1743
Bse3DI GCAATG 2 cut(s) 703, 2354
Bse8I GATNNNNATC 1 cut(s) 2742
BseBI CCWGG 6 cut(s) 314, 347, 439, 986, 3539, 3945
BseCI ATCGAT 1 cut(s) 1905
BseGI GGATG 8 cut(s) 543, 550, 649, 2806, 3187, 3739, 4118, 4855
BseJI GATNNNNATC 1 cut(s) 2742
BseLI CCNNNNNNNGG 9 cut(s) 14, 319, 538, 1490, 2090, 2206, 3391, 3464, 4088
BseMI GCAATG 2 cut(s) 703, 2354
BseRI GAGGAG 3 cut(s) 1506, 3557, 4586
BseSI GKGCMC 1 cut(s) 452
BseYI CCCAGC 1 cut(s) 1686
BsgI GTGCAG 7 cut(s) 340, 2013, 2999, 3152, 3843, 3950, 4715
Bsh1236I CGCG 3 cut(s) 660, 1155, 1452
Bsh1285I CGRYCG 1 cut(s) 4349
BshNI GGYRCC 2 cut(s) 4, 3937
BshTI ACCGGT 1 cut(s) 2732
BshVI ATCGAT 1 cut(s) 1905
BsiEI CGRYCG 1 cut(s) 4349
BsiHKAI GWGCWC 1 cut(s) 2555
BsiHKCI CYCGRG 2 cut(s) 2214, 4481
BslFI GGGAC 5 cut(s) 887, 2139, 3857, 4817, 4886
BslI CCNNNNNNNGG 9 cut(s) 14, 319, 538, 1490, 2090, 2206, 3391, 3464, 4088
BsmFI GGGAC 5 cut(s) 887, 2139, 3857, 4817, 4886
BsmI GAATGC 2 cut(s) 496, 1183
Bso31I GGTCTC 1 cut(s) 1077
BsoBI CYCGRG 2 cut(s) 2214, 4481
Bsp119I TTCGAA 3 cut(s) 3353, 3635, 3905
Bsp120I GGGCCC 1 cut(s) 448
Bsp1286I GDGCHC 2 cut(s) 452, 2555
Bsp1407I TGTACA 2 cut(s) 646, 4944
Bsp1720I GCTNAGC 1 cut(s) 4673
Bsp19I CCATGG 1 cut(s) 1016
BspDI ATCGAT 1 cut(s) 1905
BspFNI CGCG 3 cut(s) 660, 1155, 1452
BspHI TCATGA 2 cut(s) 214, 4452
BspMAI CTGCAG 2 cut(s) 792, 4570
BspMI ACCTGC 1 cut(s) 3021
BspPI GGATC 7 cut(s) 789, 1233, 2074, 2841, 3516, 4703, 4716
BspQI GCTCTTC 1 cut(s) 2560
BspT104I TTCGAA 3 cut(s) 3353, 3635, 3905
BspT107I GGYRCC 2 cut(s) 4, 3937
BspTI CTTAAG 3 cut(s) 1325, 3152, 3985
BspTNI GGTCTC 1 cut(s) 1077
BsrDI GCAATG 2 cut(s) 703, 2354
BsrFI RCCGGY 2 cut(s) 2732, 4914
BsrGI TGTACA 2 cut(s) 646, 4944
BssAI RCCGGY 2 cut(s) 2732, 4914
BssNI GRCGYC 2 cut(s) 557, 2334
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 4 cut(s) 532, 1016, 1299, 1374
Bst2BI CACGAG 1 cut(s) 776
Bst2UI CCWGG 6 cut(s) 314, 347, 439, 986, 3539, 3945
Bst6I CTCTTC 8 cut(s) 54, 285, 620, 1062, 1802, 2560, 2970, 4239
BstACI GRCGYC 2 cut(s) 557, 2334
BstAFI CTTAAG 3 cut(s) 1325, 3152, 3985
BstAPI GCANNNNNTGC 2 cut(s) 4526, 4669
BstAUI TGTACA 2 cut(s) 646, 4944
BstBAI YACGTR 4 cut(s) 328, 1590, 2887, 2919
BstBI TTCGAA 3 cut(s) 3353, 3635, 3905
BstC8I GCNNGC 9 cut(s) 355, 472, 1644, 3076, 3295, 3620, 4464, 4656, 4916
BstDSI CCRYGG 2 cut(s) 1016, 3749
BstEII GGTNACC 1 cut(s) 3302
BstF5I GGATG 8 cut(s) 543, 550, 649, 2806, 3187, 3739, 4118, 4855
BstFNI CGCG 3 cut(s) 660, 1155, 1452
BstH2I RGCGCY 2 cut(s) 3385, 4304
BstHHI GCGC 6 cut(s) 660, 1312, 1956, 2139, 3384, 4303
BstMCI CGRYCG 1 cut(s) 4349
BstNI CCWGG 6 cut(s) 314, 347, 439, 986, 3539, 3945
BstNSI RCATGY 5 cut(s) 189, 2546, 4017, 4466, 4499
BstPI GGTNACC 1 cut(s) 3302
BstSCI CCNGG 9 cut(s) 312, 345, 396, 437, 984, 3537, 3943, 4480, 4481
BstSFI CTRYAG 4 cut(s) 298, 788, 1023, 4566
BstSLI GKGCMC 1 cut(s) 452
BstSNI TACGTA 1 cut(s) 2887
BstUI CGCG 3 cut(s) 660, 1155, 1452
BstV2I GAAGAC 1 cut(s) 2849
BstX2I RGATCY 7 cut(s) 781, 1658, 2833, 3319, 3521, 4412, 4708
BstYI RGATCY 7 cut(s) 781, 1658, 2833, 3319, 3521, 4412, 4708
Bsu15I ATCGAT 1 cut(s) 1905
Bsu36I CCTNAGG 1 cut(s) 1743
BsuI GTATCC 2 cut(s) 2207, 4495
BsuTUI ATCGAT 1 cut(s) 1905
BtgI CCRYGG 2 cut(s) 1016, 3749
BtgZI GCGATG 3 cut(s) 318, 489, 675
BtsCI GGATG 8 cut(s) 543, 550, 649, 2806, 3187, 3739, 4118, 4855
BtsI GCAGTG 1 cut(s) 3962
BveI ACCTGC 1 cut(s) 3021
Cac8I GCNNGC 9 cut(s) 355, 472, 1644, 3076, 3295, 3620, 4464, 4656, 4916
CaiI CAGNNNCTG 7 cut(s) 917, 3043, 3538, 4142, 4196, 4199, 4322
CciI TCATGA 2 cut(s) 214, 4452
CfoI GCGC 6 cut(s) 660, 1312, 1956, 2139, 3384, 4303
Cfr10I RCCGGY 2 cut(s) 2732, 4914
Cfr9I CCCGGG 1 cut(s) 4481
ClaI ATCGAT 1 cut(s) 1905
CpoI CGGWCCG 1 cut(s) 384
CseI GACGC 5 cut(s) 565, 2342, 2630, 3209, 4883
CsiI ACCWGGT 1 cut(s) 345
Csp6I GTAC 9 cut(s) 647, 710, 2884, 3192, 3395, 3654, 3813, 3948, 4945
CspAI ACCGGT 1 cut(s) 2732
CspI CGGWCCG 1 cut(s) 384
CviQI GTAC 9 cut(s) 647, 710, 2884, 3192, 3395, 3654, 3813, 3948, 4945
DraI TTTAAA 2 cut(s) 279, 1795
DrdI GACNNNNNNGTC 3 cut(s) 89, 113, 1127
DriI GACNNNNNGTC 1 cut(s) 406
DseDI GACNNNNNNGTC 3 cut(s) 89, 113, 1127
EaeI YGGCCR 3 cut(s) 478, 2092, 3664
Eam1104I CTCTTC 8 cut(s) 54, 285, 620, 1062, 1802, 2560, 2970, 4239
Eam1105I GACNNNNNGTC 1 cut(s) 406
EarI CTCTTC 8 cut(s) 54, 285, 620, 1062, 1802, 2560, 2970, 4239
EciI GGCGGA 1 cut(s) 376
Eco105I TACGTA 1 cut(s) 2887
Eco130I CCWWGG 4 cut(s) 532, 1016, 1299, 1374
Eco147I AGGCCT 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 452
Eco31I GGTCTC 1 cut(s) 1077
Eco32I GATATC 1 cut(s) 1903
Eco47I GGWCC 9 cut(s) 33, 343, 384, 435, 2672, 3176, 3535, 4340, 4439
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 1743
Eco88I CYCGRG 2 cut(s) 2214, 4481
Eco91I GGTNACC 1 cut(s) 3302
EcoO109I RGGNCCY 3 cut(s) 449, 3535, 4139
EcoO65I GGTNACC 1 cut(s) 3302
EcoRII CCWGG 6 cut(s) 312, 345, 437, 984, 3537, 3943
EcoRV GATATC 1 cut(s) 1903
EcoT14I CCWWGG 4 cut(s) 532, 1016, 1299, 1374
EcoT22I ATGCAT 3 cut(s) 820, 1276, 2118
EcoT38I GRGCYC 1 cut(s) 452
ErhI CCWWGG 4 cut(s) 532, 1016, 1299, 1374
FalI AAGNNNNNCTT 6 cut(s) 3291, 3323, 4278, 4310, 4790, 4822
FaqI GGGAC 5 cut(s) 887, 2139, 3857, 4817, 4886
FauI CCCGC 5 cut(s) 463, 4033, 4467, 4922, 4930
FauNDI CATATG 5 cut(s) 103, 820, 874, 1843, 4522
FbaI TGATCA 1 cut(s) 3343
FblI GTMKAC 1 cut(s) 1295
FokI GGATG 8 cut(s) 537, 550, 656, 2793, 3194, 3746, 4105, 4862
FriOI GRGCYC 1 cut(s) 452
FspI TGCGCA 1 cut(s) 1311
GlaI GCGC 6 cut(s) 659, 1311, 1955, 2138, 3383, 4302
GsaI CCCAGC 1 cut(s) 1690
GsuI CTGGAG 4 cut(s) 2067, 3260, 3560, 3927
HaeII RGCGCY 2 cut(s) 3385, 4304
HgaI GACGC 5 cut(s) 565, 2342, 2630, 3209, 4883
HhaI GCGC 6 cut(s) 660, 1312, 1956, 2139, 3384, 4303
Hin1I GRCGYC 2 cut(s) 557, 2334
Hin6I GCGC 6 cut(s) 658, 1310, 1954, 2137, 3382, 4301
HinP1I GCGC 6 cut(s) 658, 1310, 1954, 2137, 3382, 4301
HincII GTYRAC 4 cut(s) 1335, 1513, 1726, 2257
HindII GTYRAC 4 cut(s) 1335, 1513, 1726, 2257
HindIII AAGCTT 2 cut(s) 3982, 4184
HpaI GTTAAC 3 cut(s) 1335, 1513, 1726
Hpy166II GTNNAC 5 cut(s) 1296, 1335, 1513, 1726, 2257
Hpy8I GTNNAC 5 cut(s) 1296, 1335, 1513, 1726, 2257
Hpy99I CGWCG 1 cut(s) 559
HpyCH4IV ACGT 7 cut(s) 327, 1122, 1258, 1589, 2549, 2886, 2918
HpySE526I ACGT 7 cut(s) 327, 1122, 1258, 1589, 2549, 2886, 2918
Hsp92I GRCGYC 2 cut(s) 557, 2334
HspAI GCGC 6 cut(s) 658, 1310, 1954, 2137, 3382, 4301
KroI GCCGGC 1 cut(s) 4914
KroNI GCCGGC 1 cut(s) 4916
Ksp22I TGATCA 1 cut(s) 3343
KspAI GTTAAC 3 cut(s) 1335, 1513, 1726
LguI GCTCTTC 1 cut(s) 2560
LmnI GCTCC 9 cut(s) 1214, 1493, 1564, 1672, 3050, 3241, 3766, 4471, 4831
MabI ACCWGGT 1 cut(s) 345
MaeII ACGT 7 cut(s) 327, 1122, 1258, 1589, 2549, 2886, 2918
MfeI CAATTG 1 cut(s) 1824
MflI RGATCY 7 cut(s) 781, 1658, 2833, 3319, 3521, 4412, 4708
MhlI GDGCHC 2 cut(s) 452, 2555
MlyI GAGTC 4 cut(s) 395, 929, 1160, 2591
MmeI TCCRAC 5 cut(s) 509, 578, 594, 2363, 2559
Mph1103I ATGCAT 3 cut(s) 820, 1276, 2118
MroNI GCCGGC 1 cut(s) 4914
MroXI GAANNNNTTC 1 cut(s) 4077
MslI CAYNNNNRTG 5 cut(s) 1195, 2733, 2960, 3309, 3432
MspA1I CMGCKG 5 cut(s) 1718, 2945, 4028, 4196, 4921
MspCI CTTAAG 3 cut(s) 1325, 3152, 3985
MspR9I CCNGG 9 cut(s) 314, 347, 398, 439, 986, 3539, 3945, 4482, 4483
MunI CAATTG 1 cut(s) 1824
Mva1269I GAATGC 2 cut(s) 496, 1183
MvaI CCWGG 6 cut(s) 314, 347, 439, 986, 3539, 3945
MvnI CGCG 3 cut(s) 660, 1155, 1452
NaeI GCCGGC 1 cut(s) 4916
NciI CCSGG 3 cut(s) 398, 4482, 4483
NcoI CCATGG 1 cut(s) 1016
NdeI CATATG 5 cut(s) 103, 820, 874, 1843, 4522
NgoMIV GCCGGC 1 cut(s) 4914
NmeAIII GCCGAG 2 cut(s) 4033, 4943
NmuCI GTSAC 6 cut(s) 665, 2187, 3302, 3960, 4350, 4894
NsbI TGCGCA 1 cut(s) 1311
NsiI ATGCAT 3 cut(s) 820, 1276, 2118
NspI RCATGY 5 cut(s) 189, 2546, 4017, 4466, 4499
NspV TTCGAA 3 cut(s) 3353, 3635, 3905
OliI CACNNNNGTG 2 cut(s) 2733, 3309
PaeI GCATGC 1 cut(s) 4466
PaeR7I CTCGAG 1 cut(s) 2214
PagI TCATGA 2 cut(s) 214, 4452
PceI AGGCCT 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 2560
PctI GAATGC 2 cut(s) 496, 1183
PdiI GCCGGC 1 cut(s) 4916
PdmI GAANNNNTTC 1 cut(s) 4077
PflFI GACNNNGTC 1 cut(s) 2439
PflMI CCANNNNNTGG 3 cut(s) 2090, 2206, 3464
PinAI ACCGGT 1 cut(s) 2732
PleI GAGTC 4 cut(s) 395, 928, 1160, 2590
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 4 cut(s) 395, 928, 1160, 2590
Ppu21I YACGTR 4 cut(s) 328, 1590, 2887, 2919
PpuMI RGGWCCY 1 cut(s) 3535
PshBI ATTAAT 1 cut(s) 2958
Psp5II RGGWCCY 1 cut(s) 3535
Psp6I CCWGG 6 cut(s) 312, 345, 437, 984, 3537, 3943
PspCI CACGTG 1 cut(s) 328
PspEI GGTNACC 1 cut(s) 3302
PspFI CCCAGC 1 cut(s) 1686
PspGI CCWGG 6 cut(s) 312, 345, 437, 984, 3537, 3943
PspOMI GGGCCC 1 cut(s) 448
PspPPI RGGWCCY 1 cut(s) 3535
PspXI VCTCGAGB 1 cut(s) 2214
PsrI GAACNNNNNNTAC 2 cut(s) 2703, 2735
PstI CTGCAG 2 cut(s) 792, 4570
PstNI CAGNNNCTG 7 cut(s) 917, 3043, 3538, 4142, 4196, 4199, 4322
PsuI RGATCY 7 cut(s) 781, 1658, 2833, 3319, 3521, 4412, 4708
PsyI GACNNNGTC 1 cut(s) 2439
PvuII CAGCTG 3 cut(s) 2945, 4196, 4921
RsaI GTAC 9 cut(s) 648, 711, 2885, 3193, 3396, 3655, 3814, 3949, 4946
RsaNI GTAC 9 cut(s) 647, 710, 2884, 3192, 3395, 3654, 3813, 3948, 4945
RseI CAYNNNNRTG 5 cut(s) 1195, 2733, 2960, 3309, 3432
Rsr2I CGGWCCG 1 cut(s) 384
RsrII CGGWCCG 1 cut(s) 384
SapI GCTCTTC 1 cut(s) 2560
ScaI AGTACT 1 cut(s) 3655
SchI GAGTC 4 cut(s) 395, 929, 1160, 2591
ScrFI CCNGG 9 cut(s) 314, 347, 398, 439, 986, 3539, 3945, 4482, 4483
SduI GDGCHC 2 cut(s) 452, 2555
SexAI ACCWGGT 1 cut(s) 345
SfcI CTRYAG 4 cut(s) 298, 788, 1023, 4566
Sfr274I CTCGAG 1 cut(s) 2214
SfuI TTCGAA 3 cut(s) 3353, 3635, 3905
SgrAI CRCCGGYG 1 cut(s) 2732
SinI GGWCC 9 cut(s) 33, 343, 384, 435, 2672, 3176, 3535, 4340, 4439
SlaI CTCGAG 1 cut(s) 2214
SmaI CCCGGG 1 cut(s) 4483
SmiMI CAYNNNNRTG 5 cut(s) 1195, 2733, 2960, 3309, 3432
SnaBI TACGTA 1 cut(s) 2887
SphI GCATGC 1 cut(s) 4466
SseBI AGGCCT 1 cut(s) 430
SspI AATATT 3 cut(s) 2024, 3221, 3701
StuI AGGCCT 1 cut(s) 430
StyD4I CCNGG 9 cut(s) 312, 345, 396, 437, 984, 3537, 3943, 4480, 4481
StyI CCWWGG 4 cut(s) 532, 1016, 1299, 1374
TaiI ACGT 7 cut(s) 330, 1125, 1261, 1592, 2552, 2889, 2921
TaqII GACCGA 2 cut(s) 3633, 4335
TatI WGTACW 4 cut(s) 646, 709, 3653, 4944
TauI GCSGC 4 cut(s) 334, 2097, 2282, 3387
TseFI GTSAC 6 cut(s) 665, 2187, 3302, 3960, 4350, 4894
Tsp45I GTSAC 6 cut(s) 665, 2187, 3302, 3960, 4350, 4894
TspGWI ACGGA 7 cut(s) 450, 1201, 2830, 3514, 3766, 4681, 4862
TspMI CCCGGG 1 cut(s) 4481
Tth111I GACNNNGTC 1 cut(s) 2439
Van91I CCANNNNNTGG 3 cut(s) 2090, 2206, 3464
Vha464I CTTAAG 3 cut(s) 1325, 3152, 3985
VpaK11BI GGWCC 9 cut(s) 33, 343, 384, 435, 2672, 3176, 3535, 4340, 4439
VspI ATTAAT 1 cut(s) 2958
XbaI TCTAGA 3 cut(s) 1630, 2455, 4507
XceI RCATGY 5 cut(s) 189, 2546, 4017, 4466, 4499
XcmI CCANNNNNNNNNTGG 1 cut(s) 992
XhoI CTCGAG 1 cut(s) 2214
XmaI CCCGGG 1 cut(s) 4481
XmaJI CCTAGG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 1295
XmnI GAANNNNTTC 1 cut(s) 4077
ZrmI AGTACT 1 cut(s) 3655
Zsp2I ATGCAT 3 cut(s) 820, 1276, 2118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.