FvH4_6g29390

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004812
Physical Location & Seq
Reverse (-)
22660239 .. 22670712
10474 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29390.t6

Sequence Viewer

Length: 4998 bp
ATGGCCCCAAAATCAGGCAGAGGCAAAAACAACAAGGCCAAGTCTGATAAGAAGAAGAAGGAAGAGAAGGTTCCTAGTGTTCTTGACATCACTATCATTACTCCATATGATACACAAGTTATTCTCAAGGGTATCTCCACTGACAAGATACTCGATGTTAAAAGACTACTGGCTGTGAATGTAGAGACATGTCATCTTACAAACTATTCCTTGTCTCATGAAGTGAAAGGGAAGAGACTGAATGAAAGGGTGGAGGTGGTTAGTCTGAAACCCTGTCTTCTGAAAATGGTTGAAGAGGACTACACCGAAGAAGCCGAAGCAGTTGCGCACGTGCGGCGTCTGCTGGACCTGGTGGCCTGCACGACCCGATTCGCCAAGCCCAAACGCTCCGCTTCCGCCCCGGACTCCAAGTCCAAGAAGACCGCCGCCGCCACCACCAAGCCACTCACCAAAAGCTCCGCCCCACCCTCACCGTCCGATGGCGCAACACCCGCGCAATCCGAGCCGTCCGTCTCGGCGATCTCGGAGAGCCTCGGGATGGTCGCCATTCACCCGACCCCGAAGCTCTCCGACTTCTACGACTTCTTCTCTTTCTCTCACCTCACGCCTCCCATTCTCCATTTACGGAGGTGTGACGTGGACGACACGCGCGACGGTGACTATTTTCAAATGCAGATTAAGATTTGCAATGGGAAGCAAATACAAGTGGTTGCATCACTGAAAGGGTTCTACACAGTGGGAAAGCAGTTTTTACAAAGCCACTCATTGGTGGATCTTCTGCAACAGCTCAGTAGAGCTTTTGCTAATGCATATGAATCCTTGATGAAAGCTTTTGTAGATCATAACAAGTTTGGTGACCTTCCATATGGTTTTCGAGCAAACACATGGCTTGTTCCTCCATCTGTTGCCGAGTCTCCATCAATTTTTCCATCCCTGCCAACTGAAGATGAAAATTGGGGTGGAAATGGTGGAGGTCAGGGAAGAAATGGTGAATATGATCATCAGCAATGGGCTACTGATTTTGCAATTTTGGCTTGCATGCCTTGCAAAACTGAGGAGGAGAGGGTTGTTCGAGACCGAAAAGCATTTTTGCTTCATACTAGATTCATTGATGTAGCAATCTTCAAAGCTGCTGCAGCAATACGTGGTATAATAGATTCTACTGTGAATGCAGAAGAGACAACCAATTGTTCTCCGGGTTCTGTTCTTTATGATGAACGTGTCGGAGACTTGTCAATTGTTGTAAAACGTGATATAACAGATCCATTGTCAAACTCTGAAGTAAAAGTTAGTGGTGATCATCTATGCAGTACCAAGGAAGTTGCTCAGAGGTGTTTACTCAAGGGTTTAACTTCGGATGAGAGTGTGGTTGTTCATGATACTCCCTCCTTATGTGTGGTCAATGTGAGGCACTGTGGATACATTGCAACAGTTAAAGTTGTTGGTAAAATAAAGAAGGGAAGCTATGAGGCTAAAGATATTGACATTGAAGATCAACCAGATGGAGGAGCCAATTCTCTTAATCTCAATAGCCTCAGGGTTCTGCTTCAAAAGTTCAACACTGAATCAGTGGATAACTCTAATTCAGATGGTTTAAAAAATTCAAGGTCTCTGGTTCGAAGAGTAATAAAAGAGAGCTTGACAAAGTTAGAGGACGAGCCTGCCAATTCTGAGAGGTCTATTAGATGGGAACTTGGTTCTTGCTGGTTGCAACATTTACTTAAGCAGGAAACACCAGTAAACAATTCTGATAGTCCTGAGGATGATAATGAGGTGGCTGCACCAGTTGTTAAAGGTCTAGGGAAGCAATTTAAATTTTTGAAGAAGAGGGAAAAGAAAACAAGTACTGAAGCTGGAACAAATGATGAGGAAGATATAGATGCTAGTGCTCTCAGTCTAAATGGGGAATCAGATAAATTAGAGCTAAATAATGGGGGGAGCCACGAAATCAGCAGCAATGAGGCAGAATTGAAGACTTTAATTTCAGAAGAAGCCTATTTGCGTCTGAAAGAAAGTGGAACTAATCTTCATCTAAAGTCAGCGGATGAGCTAATGAAGATGGCATACAAATATTATGATGAAGTTGCCTTGCCAAAGCTGGTAACAGACTTTGGATCACTTGAACTTTCTCCAGTTGACGGCCGTACGCTGACAGATTTCATGCATTTAAGGGGGCTGAAAATGCAGTCTTTGGGTCGTGTGGTTGAACTTTCGGAGAAACTTCCTCATATACAGTCTCTTTGTATTCATGAGATGATTACTCGAGCTTTCAAGCATGTGGTTGAAGCAGTGGTGGCTTCTGTTGGCAAAATAACTGACTTGTCTGCGGCAATAGCTGCAACCCTGAATTTTCTACTTGGAGGCTCTGGGATGGATGATGATGTCCTTAAATTGCAATGGTTACGAATATTCCTGGCCCGAAAATTTGGTTGGTCATTGAAAGATGAATTTCAGCACTTGAGGAAGTTGTCAATTCTCCGAGGACTTTGCCGTAAGGTTGGGCTGGAGTTGGCCCCACGAGATTATGACATGGAATGTCATAACCCTTTCAGCAAATATGACATTATTAGCATGGTTCCAGTGTGTAAGCATGTAGCATGCTCTTCAGCTGATGGACGAAATCTGTTGGAGTCATCTAAAATTGCTCTTGATAAAGGAAAGTTAGATGATGCTGTACATTACGGAACAAAGGCACTAGCGAAGATGATAGCTGTCTGTGGTCACTATCATCGAGTTACTGCAAGTGCTTACAGCCTTTTAGCAGTAGTTCTTTACCACACGGGTGATTTTAATCAGGCAACCATTTATCAGCAGAAGGCTTTAGCTATTAATGAAAGGGAACTTGGGCTGGACCATCCTGATACCATGAAAAGCTATGGGGATCTTTCTGTTTTTTACTACCGTCTTCAATACATCGAATTGGCTTTGAAGTATGTAAATCGTGCATTGTTTCTTCTTCATTTTACATGTGGACTATCTCACCCTAACACAGCTGCGACATATATAAATGTGGCTATGATGGAGGAAGGCATGGGAAATGTCCATGTTGCTCTCAGATACCTACATGAAGCTCTCAAGTGCAACCAGAGACTATTAGGAGCAGATCACATACAGACTGCTGCAAGCTATCATGCCATAGCCATAGCTCTTTCATTGATGGAAGCATATTCTCTAAGCGTGCAACATGAGCAAACAACACTCAAGATACTTCAAGACAAACTTGGACCTGAGGATCTTCGTACTCAGGATGCAGCAGCATGGCTTGAATATTTTGAGTCTAAGTCTCTAGAGCAGCAAGAAGCAGCACGAAATGGATCTCCAAAGCCTGATGCCTTGATTGCAAGCAAAGGTCACCTTAGTGTGTCAGATCTCCTGGATTACATAAGCCCTGATCAAGATTCAAAAGTAAATGATGCACATAGGAAGCAGCGACGTGCAAAGGTTCATCAATCGAGTGATACTATCTATCAGGAACATCAAGATGCGGTAGGTGAAGATGACTTGCCAAGTGATGGACTTGAAAACACTATGGTCTTGATAGATGATAATACTGAAGTAGTTGAGGAAAGATGGGTTCATCAGGAACTGGAGGAAAAAGAACATGTCTCTAGAAATGGTTTGTCAGTTACCGGTGTAACAGCCGAAGAAACAACTTCAGATGAAGGCTGGCAAGAAGCCAATTCAAAGGGGAGGTCTGGAAATACCACTTCTGGGCGGAAGTTCAGTCGAAGGAGGCCAGATAGTGAATCTTCACACTTCAGAGAAAGCAAATATTCGAGGGAAGTTAAATCCTCATCACAGACTGCAGCTGCGAAGAGCTTTCTAAATGATTCATCCTCATCAAAGCAATCAAAGGTTCGTACTGTGAGTACTGGAGAGGACTCAGTTAGACTTCAGTCCAAGAATACTGTTTCTAAAGTCTCCACTACTCCAGCGACTCTTACGAACTTGACTTCAAAATCTGTATCTTACAAACAAGTAGCTTTGGCACCTCCAGGTACTGTCCTAAAGTCATTGCTGGATAAAGTTGACAACCTAAATGTGGAAAAATCTGAAAAAAAGGTGTGTAACCCTCCACCTGAGACATTGAAAACTGAAGAAAGCATAGGGGTAGTTGAATTCACTCCAAAGGACGAGACCGAAGGAACACATGCAAGTTCAACCCCATCAGAAGATACAGGCCCTGAAACTGTGGAAGAAAGGTCTGCCGAAAAAAATGGAAGTAAGCTATCGGCTGCAGCAGAACCATTCAATCCAAGACCACACACCATGGCTCACCCTCTAAATCCAGTTGCTGTTACAAGTGTATATGATGTGAGAGCCAGTCAAGCAATGCTTTCTGCACCTGTGCTACCCCCAGTAGCTGCCAGAGTTCCTTGTGGTCCTCGGTCACCTTTGTATTATAAAACCAACTATTCTTTTCGCATGAGACAAGGTGTTCAAAAGTTTCAAAGTCCCCTTTCAGAAAGAAGTGGATCTGGACCTCCAAGAATCATGAATCCACATGCTCCAGAGTTTGTTCCCGGGAGAAGTTTGCCAGCAGATTACATGGATGAATATGCAGAATATGCAACTGATTCAAATTCTTCATTTGAAATGAATAGAGCTGAAGAGATGGATGAGAAATCAAATGGTAAAGCCGAGAGAAAGAGCATTTCTGAGTCTGAGAAGTCAGAGCTTGCAAGGCAGATATTGCTGAGCTTTATCGTAAAATCTGTCCAGCACAACAAGGATTCGGAAAGTGAAAGTAAGCCGGAAAATCATTCTGATGCTGTAGAGAATGACAGTGCAATTATAAAAATTCACTACGGAAATGAAGGAAAAACGAACTTGGTTTCTCAATCCAGTGACTGTGATCAATCAAAAACAATGGATGTGGATACAAATGAAGTTGTTGATAGTGAAGGATTTACAGTTGTGACAAAGAGGAGAAGAAGTCGGCAGCAGTTCAGAAGTGAGTTGTACAACCAGCAATCCATCTCAGCTTCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1666

Amino Acids

184.26

Weight (kDa)

6.08

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 47 - 118 2.6e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 709 - 789 2.2e-16 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 882 - 945 1.2e-08 Tetratricopeptide repeat
TPR_12 PF13424 913 - 983 6.7e-11 Tetratricopeptide repeat
TPR_10 PF13374 913 - 954 2.5e-10 Tetratricopeptide repeat
TPR_12 PF13424 997 - 1071 3.5e-11 Tetratricopeptide repeat
TPR_10 PF13374 997 - 1037 3e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4404, 4796
Acc16I TGCGCA 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 3988
AccB7I CCANNNNNTGG 2 cut(s) 766, 3512
AccII CGCG 3 cut(s) 494, 649, 651
AclWI GGATC 7 cut(s) 780, 1256, 2122, 2889, 3240, 3322, 4483
AcoI YGGCCR 1 cut(s) 2140
AcsI RAATTY 8 cut(s) 1600, 1814, 2347, 2423, 2447, 4118, 4582, 4800
AcvI CACGTG 1 cut(s) 331
AcyI GRCGYC 1 cut(s) 337
AfaI GTAC 9 cut(s) 1312, 1846, 2146, 2676, 3241, 3862, 3871, 4000, 4964
AfiI CCNNNNNNNGG 9 cut(s) 14, 479, 538, 766, 1505, 2138, 3512, 3711, 4042
AflII CTTAAG 1 cut(s) 1721
AflIII ACRYGT 4 cut(s) 188, 1219, 2966, 3601
AgeI ACCGGT 1 cut(s) 3629
AhdI GACNNNNNGTC 1 cut(s) 409
AjiI CACGTC 2 cut(s) 637, 3434
AjnI CCWGG 4 cut(s) 348, 2412, 3372, 3994
AjuI GAANNNNNNNTTGG 4 cut(s) 2413, 2445, 2826, 2858
AleI CACNNNNGTG 2 cut(s) 2781, 3357
Alw21I GWGCWC 1 cut(s) 1891
AlwI GGATC 7 cut(s) 780, 1256, 2122, 2889, 3240, 3322, 4483
AlwNI CAGNNNCTG 7 cut(s) 3586, 4001, 4184, 4295, 4364, 4851, 4988
Ama87I CYCGRG 3 cut(s) 533, 2262, 4523
AoxI GGCC 8 cut(s) 3, 36, 354, 2140, 2415, 2511, 3734, 4180
ApoI RAATTY 8 cut(s) 1600, 1814, 2347, 2423, 2447, 4118, 4582, 4800
ArsI GACNNNNNNTTYG 2 cut(s) 3677, 3709
AseI ATTAAT 1 cut(s) 2829
AsiGI ACCGGT 1 cut(s) 3629
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 4 cut(s) 328, 485, 496, 651
AspS9I GGNCC 9 cut(s) 4, 346, 2416, 2512, 2851, 3224, 4181, 4382, 4481
AsuC2I CCSGG 4 cut(s) 401, 1197, 4524, 4525
AsuII TTCGAA 1 cut(s) 1618
AvaI CYCGRG 3 cut(s) 533, 2262, 4523
AvaII GGWCC 5 cut(s) 346, 2851, 3224, 4382, 4481
AxyI CCTNAGG 3 cut(s) 1535, 1758, 3228
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BanI GGYRCC 1 cut(s) 3988
BarI GAAGNNNNNNTAC 2 cut(s) 2048, 2080
BauI CACGAG 1 cut(s) 2517
BbrPI CACGTG 1 cut(s) 331
BbsI GAAGAC 4 cut(s) 269, 425, 1979, 2897
Bbv12I GWGCWC 1 cut(s) 1891
BceAI ACGGC 4 cut(s) 490, 2127, 2155, 2475
BcgI CGANNNNNNTGC 4 cut(s) 305, 339, 2469, 2503
BciT130I CCWGG 4 cut(s) 350, 2414, 3374, 3996
BciVI GTATCC 2 cut(s) 1412, 4873
BclI TGATCA 4 cut(s) 997, 1297, 3391, 4855
BcnI CCSGG 4 cut(s) 401, 1197, 4524, 4525
BfaI CTAG 7 cut(s) 75, 1101, 1799, 1884, 2696, 3287, 3609
BfmI CTRYAG 4 cut(s) 1134, 3804, 4236, 4773
BfrI CTTAAG 1 cut(s) 1721
BfuI GTATCC 2 cut(s) 1412, 4873
BglII AGATCT 1 cut(s) 3367
BlpI GCTNAGC 1 cut(s) 4697
BmcAI AGTACT 2 cut(s) 1846, 3871
Bme1390I CCNGG 8 cut(s) 350, 401, 1197, 2414, 3374, 3996, 4524, 4525
Bme18I GGWCC 5 cut(s) 346, 2851, 3224, 4382, 4481
BmeRI GACNNNNNGTC 1 cut(s) 409
BmeT110I CYCGRG 3 cut(s) 533, 2262, 4523
BmgBI CACGTC 2 cut(s) 637, 3434
BmgT120I GGNCC 9 cut(s) 4, 346, 2416, 2512, 2851, 3224, 4181, 4382, 4481
BmiI GGNNCC 7 cut(s) 6, 72, 1510, 1940, 2514, 2577, 3990
BmrFI CCNGG 8 cut(s) 350, 401, 1197, 2414, 3374, 3996, 4524, 4525
BmrI ACTGGG 1 cut(s) 4352
BmsI GCATC 8 cut(s) 722, 1870, 2659, 3238, 3319, 3403, 3472, 4759
BmuI ACTGGG 1 cut(s) 4352
BoxI GACNNNNGTC 1 cut(s) 3895
BpiI GAAGAC 4 cut(s) 269, 425, 1979, 2897
BpmI CTGGAG 7 cut(s) 2115, 2525, 3608, 3894, 3915, 3978, 4494
Bpu1102I GCTNAGC 1 cut(s) 4697
Bpu14I TTCGAA 1 cut(s) 1618
BpuEI CTTGAG 5 cut(s) 110, 1325, 2479, 3059, 3185
BpuMI CCSGG 4 cut(s) 401, 1197, 4524, 4525
BsaAI YACGTR 2 cut(s) 331, 1145
BsaBI GATNNNNATC 1 cut(s) 2790
BsaHI GRCGYC 1 cut(s) 337
BsaI GGTCTC 3 cut(s) 1068, 1614, 4130
BsaJI CCNNGG 7 cut(s) 399, 532, 1314, 2479, 4269, 4385, 4523
BsaWI WCCGGW 1 cut(s) 3629
BsaXI ACNNNNNCTCC 2 cut(s) 4921, 4951
Bsc4I CCNNNNNNNGG 9 cut(s) 14, 479, 538, 766, 1505, 2138, 3512, 3711, 4042
Bse118I RCCGGY 1 cut(s) 3629
Bse21I CCTNAGG 3 cut(s) 1535, 1758, 3228
Bse3DI GCAATG 7 cut(s) 694, 1013, 1422, 1963, 2402, 4013, 4338
Bse8I GATNNNNATC 1 cut(s) 2790
BseBI CCWGG 4 cut(s) 350, 2414, 3374, 3996
BseDI CCNNGG 7 cut(s) 399, 532, 1314, 2479, 4269, 4385, 4523
BseJI GATNNNNATC 1 cut(s) 2790
BseLI CCNNNNNNNGG 9 cut(s) 14, 479, 538, 766, 1505, 2138, 3512, 3711, 4042
BseMI GCAATG 7 cut(s) 694, 1013, 1422, 1963, 2402, 4013, 4338
BseRI GAGGAG 4 cut(s) 1070, 1073, 1521, 4942
BseX3I CGGCCG 1 cut(s) 2140
BsgI GTGCAG 3 cut(s) 343, 1764, 4326
Bsh1236I CGCG 3 cut(s) 494, 649, 651
Bsh1285I CGRYCG 1 cut(s) 2143
BshFI GGCC 8 cut(s) 5, 38, 356, 2142, 2417, 2513, 3736, 4182
BshNI GGYRCC 1 cut(s) 3988
BshTI ACCGGT 1 cut(s) 3629
BsiEI CGRYCG 1 cut(s) 2143
BsiHKAI GWGCWC 1 cut(s) 1891
BsiHKCI CYCGRG 3 cut(s) 533, 2262, 4523
BsiSI CCGG 5 cut(s) 401, 1196, 3630, 4524, 4754
BsiWI CGTACG 1 cut(s) 2144
BslFI GGGAC 1 cut(s) 4440
BslI CCNNNNNNNGG 9 cut(s) 14, 479, 538, 766, 1505, 2138, 3512, 3711, 4042
BsmBI CGTCTC 1 cut(s) 517
BsmFI GGGAC 1 cut(s) 4440
BsmI GAATGC 1 cut(s) 1174
BsnI GGCC 8 cut(s) 5, 38, 356, 2142, 2417, 2513, 3736, 4182
Bso31I GGTCTC 3 cut(s) 1068, 1614, 4130
BsoBI CYCGRG 3 cut(s) 533, 2262, 4523
Bsp119I TTCGAA 1 cut(s) 1618
Bsp1286I GDGCHC 1 cut(s) 1891
Bsp1407I TGTACA 2 cut(s) 2674, 4962
Bsp1720I GCTNAGC 1 cut(s) 4697
Bsp19I CCATGG 1 cut(s) 4269
BspANI GGCC 8 cut(s) 5, 38, 356, 2142, 2417, 2513, 3736, 4182
BspFNI CGCG 3 cut(s) 494, 649, 651
BspHI TCATGA 4 cut(s) 217, 1375, 2248, 4494
BspLI GGNNCC 7 cut(s) 6, 72, 1510, 1940, 2514, 2577, 3990
BspMAI CTGCAG 3 cut(s) 1138, 3808, 4240
BspPI GGATC 7 cut(s) 780, 1256, 2122, 2889, 3240, 3322, 4483
BspQI GCTCTTC 2 cut(s) 2608, 3809
BspT104I TTCGAA 1 cut(s) 1618
BspT107I GGYRCC 1 cut(s) 3988
BspTI CTTAAG 1 cut(s) 1721
BspTNI GGTCTC 3 cut(s) 1068, 1614, 4130
BsrDI GCAATG 7 cut(s) 694, 1013, 1422, 1963, 2402, 4013, 4338
BsrFI RCCGGY 1 cut(s) 3629
BsrGI TGTACA 2 cut(s) 2674, 4962
BssAI RCCGGY 1 cut(s) 3629
BssECI CCNNGG 7 cut(s) 399, 532, 1314, 2479, 4269, 4385, 4523
BssNI GRCGYC 1 cut(s) 337
BssSI CACGAG 1 cut(s) 2517
BssT1I CCWWGG 2 cut(s) 1314, 4269
Bst2BI CACGAG 1 cut(s) 2517
Bst2UI CCWGG 4 cut(s) 350, 2414, 3374, 3996
Bst6I CTCTTC 9 cut(s) 57, 227, 288, 1170, 1615, 1820, 2608, 3809, 4605
BstACI GRCGYC 1 cut(s) 337
BstAFI CTTAAG 1 cut(s) 1721
BstAPI GCANNNNNTGC 4 cut(s) 1044, 2336, 4568, 4693
BstAUI TGTACA 2 cut(s) 2674, 4962
BstBAI YACGTR 2 cut(s) 331, 1145
BstBI TTCGAA 1 cut(s) 1618
BstDSI CCRYGG 1 cut(s) 4269
BstEII GGTNACC 3 cut(s) 854, 3350, 4389
BstFNI CGCG 3 cut(s) 494, 649, 651
BstHHI GCGC 4 cut(s) 328, 485, 496, 651
BstMCI CGRYCG 1 cut(s) 2143
BstNI CCWGG 4 cut(s) 350, 2414, 3374, 3996
BstNSI RCATGY 9 cut(s) 192, 1042, 2279, 2594, 2601, 2970, 3605, 4154, 4508
BstPAI GACNNNNGTC 1 cut(s) 3895
BstPI GGTNACC 3 cut(s) 854, 3350, 4389
BstSCI CCNGG 8 cut(s) 348, 399, 1195, 2412, 3372, 3994, 4522, 4523
BstSFI CTRYAG 4 cut(s) 1134, 3804, 4236, 4773
BstUI CGCG 3 cut(s) 494, 649, 651
BstV2I GAAGAC 4 cut(s) 269, 425, 1979, 2897
BstX2I RGATCY 7 cut(s) 772, 1261, 2881, 3232, 3314, 3367, 4475
BstYI RGATCY 7 cut(s) 772, 1261, 2881, 3232, 3314, 3367, 4475
BstZI CGGCCG 1 cut(s) 2140
Bsu36I CCTNAGG 3 cut(s) 1535, 1758, 3228
BsuI GTATCC 2 cut(s) 1412, 4873
BsuRI GGCC 8 cut(s) 5, 38, 356, 2142, 2417, 2513, 3736, 4182
BtgI CCRYGG 1 cut(s) 4269
BtrI CACGTC 2 cut(s) 637, 3434
BtsI GCAGTG 1 cut(s) 2295
CaiI CAGNNNCTG 7 cut(s) 3586, 4001, 4184, 4295, 4364, 4851, 4988
CciI TCATGA 4 cut(s) 217, 1375, 2248, 4494
CfoI GCGC 4 cut(s) 328, 485, 496, 651
Cfr10I RCCGGY 1 cut(s) 3629
Cfr13I GGNCC 9 cut(s) 4, 346, 2416, 2512, 2851, 3224, 4181, 4382, 4481
Cfr9I CCCGGG 1 cut(s) 4523
CseI GACGC 2 cut(s) 326, 1991
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 9 cut(s) 1311, 1845, 2145, 2675, 3240, 3861, 3870, 3999, 4963
CspAI ACCGGT 1 cut(s) 3629
CspCI CAANNNNNGTGG 2 cut(s) 4857, 4892
CviQI GTAC 9 cut(s) 1311, 1845, 2145, 2675, 3240, 3861, 3870, 3999, 4963
DraI TTTAAA 2 cut(s) 1596, 1813
DriI GACNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 2140
EagI CGGCCG 1 cut(s) 2140
Eam1104I CTCTTC 9 cut(s) 57, 227, 288, 1170, 1615, 1820, 2608, 3809, 4605
Eam1105I GACNNNNNGTC 1 cut(s) 409
EarI CTCTTC 9 cut(s) 57, 227, 288, 1170, 1615, 1820, 2608, 3809, 4605
EciI GGCGGA 3 cut(s) 385, 448, 3730
EclXI CGGCCG 1 cut(s) 2140
Eco130I CCWWGG 2 cut(s) 1314, 4269
Eco31I GGTCTC 3 cut(s) 1068, 1614, 4130
Eco47I GGWCC 5 cut(s) 346, 2851, 3224, 4382, 4481
Eco52I CGGCCG 1 cut(s) 2140
Eco72I CACGTG 1 cut(s) 331
Eco81I CCTNAGG 3 cut(s) 1535, 1758, 3228
Eco88I CYCGRG 3 cut(s) 533, 2262, 4523
Eco91I GGTNACC 3 cut(s) 854, 3350, 4389
EcoO109I RGGNCCY 1 cut(s) 4181
EcoO65I GGTNACC 3 cut(s) 854, 3350, 4389
EcoRI GAATTC 1 cut(s) 4118
EcoRII CCWGG 4 cut(s) 348, 2412, 3372, 3994
EcoT14I CCWWGG 2 cut(s) 1314, 4269
EcoT22I ATGCAT 2 cut(s) 811, 2166
ErhI CCWWGG 2 cut(s) 1314, 4269
Esp3I CGTCTC 1 cut(s) 517
FalI AAGNNNNNCTT 6 cut(s) 3204, 3236, 3339, 3371, 4320, 4352
FaqI GGGAC 1 cut(s) 4440
FauI CCCGC 1 cut(s) 499
FauNDI CATATG 3 cut(s) 106, 811, 865
FbaI TGATCA 4 cut(s) 997, 1297, 3391, 4855
FspBI CTAG 7 cut(s) 75, 1101, 1799, 1884, 2696, 3287, 3609
FspI TGCGCA 1 cut(s) 327
GlaI GCGC 4 cut(s) 327, 484, 495, 650
GsuI CTGGAG 7 cut(s) 2115, 2525, 3608, 3894, 3915, 3978, 4494
HaeIII GGCC 8 cut(s) 5, 38, 356, 2142, 2417, 2513, 3736, 4182
HapII CCGG 5 cut(s) 401, 1196, 3630, 4524, 4754
HgaI GACGC 2 cut(s) 326, 1991
HhaI GCGC 4 cut(s) 328, 485, 496, 651
Hin1I GRCGYC 1 cut(s) 337
Hin6I GCGC 4 cut(s) 326, 483, 494, 649
HinP1I GCGC 4 cut(s) 326, 483, 494, 649
HincII GTYRAC 2 cut(s) 2137, 4030
HindII GTYRAC 2 cut(s) 2137, 4030
HindIII AAGCTT 1 cut(s) 828
HpaII CCGG 5 cut(s) 401, 1196, 3630, 4524, 4754
Hpy166II GTNNAC 6 cut(s) 640, 1337, 1741, 2137, 2972, 4030
Hpy8I GTNNAC 6 cut(s) 640, 1337, 1741, 2137, 2972, 4030
Hpy99I CGWCG 2 cut(s) 656, 3435
HpyCH4IV ACGT 6 cut(s) 330, 636, 1144, 1219, 1249, 3433
HpySE526I ACGT 6 cut(s) 330, 636, 1144, 1219, 1249, 3433
Hsp92I GRCGYC 1 cut(s) 337
HspAI GCGC 4 cut(s) 326, 483, 494, 649
Ksp22I TGATCA 4 cut(s) 997, 1297, 3391, 4855
LguI GCTCTTC 2 cut(s) 2608, 3809
LmnI GCTCC 6 cut(s) 392, 461, 1508, 1938, 3098, 4513
LweI GCATC 8 cut(s) 722, 1870, 2659, 3238, 3319, 3403, 3472, 4759
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 7 cut(s) 75, 1101, 1799, 1884, 2696, 3287, 3609
MaeII ACGT 6 cut(s) 330, 636, 1144, 1219, 1249, 3433
MfeI CAATTG 2 cut(s) 1186, 1236
MflI RGATCY 7 cut(s) 772, 1261, 2881, 3232, 3314, 3367, 4475
MhlI GDGCHC 1 cut(s) 1891
MlyI GAGTC 7 cut(s) 398, 920, 2639, 3284, 3875, 3931, 4670
MmeI TCCRAC 3 cut(s) 594, 1204, 2607
Mph1103I ATGCAT 2 cut(s) 811, 2166
MroXI GAANNNNTTC 1 cut(s) 725
MslI CAYNNNNRTG 4 cut(s) 2781, 3357, 3480, 4767
MspA1I CMGCKG 4 cut(s) 2042, 2609, 2993, 3809
MspCI CTTAAG 1 cut(s) 1721
MspI CCGG 5 cut(s) 401, 1196, 3630, 4524, 4754
MspR9I CCNGG 8 cut(s) 350, 401, 1197, 2414, 3374, 3996, 4524, 4525
MunI CAATTG 2 cut(s) 1186, 1236
Mva1269I GAATGC 1 cut(s) 1174
MvaI CCWGG 4 cut(s) 350, 2414, 3374, 3996
MvnI CGCG 3 cut(s) 494, 649, 651
NciI CCSGG 4 cut(s) 401, 1197, 4524, 4525
NcoI CCATGG 1 cut(s) 4269
NdeI CATATG 3 cut(s) 106, 811, 865
NlaIV GGNNCC 7 cut(s) 6, 72, 1510, 1940, 2514, 2577, 3990
NmeAIII GCCGAG 3 cut(s) 494, 934, 4666
NmuCI GTSAC 8 cut(s) 632, 656, 854, 2720, 3350, 4389, 4847, 4918
NsbI TGCGCA 1 cut(s) 327
NsiI ATGCAT 2 cut(s) 811, 2166
NspI RCATGY 9 cut(s) 192, 1042, 2279, 2594, 2601, 2970, 3605, 4154, 4508
NspV TTCGAA 1 cut(s) 1618
OliI CACNNNNGTG 2 cut(s) 2781, 3357
PaeI GCATGC 2 cut(s) 1042, 2601
PaeR7I CTCGAG 1 cut(s) 2262
PagI TCATGA 4 cut(s) 217, 1375, 2248, 4494
PciI ACATGT 3 cut(s) 188, 2966, 3601
PciSI GCTCTTC 2 cut(s) 2608, 3809
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PctI GAATGC 1 cut(s) 1174
PdmI GAANNNNTTC 1 cut(s) 725
Pfl23II CGTACG 1 cut(s) 2144
PflMI CCANNNNNTGG 2 cut(s) 766, 3512
PfoI TCCNGGA 1 cut(s) 3372
PinAI ACCGGT 1 cut(s) 3629
PleI GAGTC 7 cut(s) 398, 919, 2638, 3283, 3875, 3931, 4669
PmaCI CACGTG 1 cut(s) 331
PmlI CACGTG 1 cut(s) 331
PpsI GAGTC 7 cut(s) 398, 919, 2638, 3283, 3875, 3931, 4669
Ppu21I YACGTR 2 cut(s) 331, 1145
PscI ACATGT 3 cut(s) 188, 2966, 3601
PshAI GACNNNNGTC 1 cut(s) 3895
PshBI ATTAAT 1 cut(s) 2829
PsiI TTATAA 2 cut(s) 4404, 4796
Psp6I CCWGG 4 cut(s) 348, 2412, 3372, 3994
PspCI CACGTG 1 cut(s) 331
PspEI GGTNACC 3 cut(s) 854, 3350, 4389
PspGI CCWGG 4 cut(s) 348, 2412, 3372, 3994
PspLI CGTACG 1 cut(s) 2144
PspN4I GGNNCC 7 cut(s) 6, 72, 1510, 1940, 2514, 2577, 3990
PspPI GGNCC 9 cut(s) 4, 346, 2416, 2512, 2851, 3224, 4181, 4382, 4481
PspXI VCTCGAGB 1 cut(s) 2262
PstI CTGCAG 3 cut(s) 1138, 3808, 4240
PstNI CAGNNNCTG 7 cut(s) 3586, 4001, 4184, 4295, 4364, 4851, 4988
PsuI RGATCY 7 cut(s) 772, 1261, 2881, 3232, 3314, 3367, 4475
PvuII CAGCTG 3 cut(s) 2609, 2993, 3809
RsaI GTAC 9 cut(s) 1312, 1846, 2146, 2676, 3241, 3862, 3871, 4000, 4964
RsaNI GTAC 9 cut(s) 1311, 1845, 2145, 2675, 3240, 3861, 3870, 3999, 4963
RseI CAYNNNNRTG 4 cut(s) 2781, 3357, 3480, 4767
SapI GCTCTTC 2 cut(s) 2608, 3809
Sau96I GGNCC 9 cut(s) 4, 346, 2416, 2512, 2851, 3224, 4181, 4382, 4481
ScaI AGTACT 2 cut(s) 1846, 3871
SchI GAGTC 7 cut(s) 398, 920, 2639, 3284, 3875, 3931, 4670
ScrFI CCNGG 8 cut(s) 350, 401, 1197, 2414, 3374, 3996, 4524, 4525
SduI GDGCHC 1 cut(s) 1891
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 8 cut(s) 722, 1870, 2659, 3238, 3319, 3403, 3472, 4759
SfcI CTRYAG 4 cut(s) 1134, 3804, 4236, 4773
Sfr274I CTCGAG 1 cut(s) 2262
SfuI TTCGAA 1 cut(s) 1618
SinI GGWCC 5 cut(s) 346, 2851, 3224, 4382, 4481
SlaI CTCGAG 1 cut(s) 2262
SmaI CCCGGG 1 cut(s) 4525
SmiI ATTTAAAT 1 cut(s) 1813
SmiMI CAYNNNNRTG 4 cut(s) 2781, 3357, 3480, 4767
SmlI CTYRAG 7 cut(s) 125, 1340, 1721, 2262, 2458, 3074, 3200
SmoI CTYRAG 7 cut(s) 125, 1340, 1721, 2262, 2458, 3074, 3200
SphI GCATGC 2 cut(s) 1042, 2601
SspI AATATT 4 cut(s) 2072, 2409, 3269, 3773
SspMI CTAG 7 cut(s) 75, 1101, 1799, 1884, 2696, 3287, 3609
StyD4I CCNGG 8 cut(s) 348, 399, 1195, 2412, 3372, 3994, 4522, 4523
StyI CCWWGG 2 cut(s) 1314, 4269
SwaI ATTTAAAT 1 cut(s) 1813
TaiI ACGT 6 cut(s) 333, 639, 1147, 1222, 1252, 3436
TaqII GACCGA 3 cut(s) 1092, 4154, 4377
TatI WGTACW 4 cut(s) 1844, 2674, 3869, 4962
TauI GCSGC 4 cut(s) 337, 428, 431, 2330
TseFI GTSAC 8 cut(s) 632, 656, 854, 2720, 3350, 4389, 4847, 4918
Tsp45I GTSAC 8 cut(s) 632, 656, 854, 2720, 3350, 4389, 4847, 4918
TspGWI ACGGA 4 cut(s) 499, 640, 2697, 4824
TspMI CCCGGG 1 cut(s) 4523
Van91I CCANNNNNTGG 2 cut(s) 766, 3512
Vha464I CTTAAG 1 cut(s) 1721
VpaK11BI GGWCC 5 cut(s) 346, 2851, 3224, 4382, 4481
VspI ATTAAT 1 cut(s) 2829
XapI RAATTY 8 cut(s) 1600, 1814, 2347, 2423, 2447, 4118, 4582, 4800
XbaI TCTAGA 2 cut(s) 3286, 3608
XceI RCATGY 9 cut(s) 192, 1042, 2279, 2594, 2601, 2970, 3605, 4154, 4508
XhoI CTCGAG 1 cut(s) 2262
XmaI CCCGGG 1 cut(s) 4523
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 7 cut(s) 75, 1101, 1799, 1884, 2696, 3287, 3609
ZrmI AGTACT 2 cut(s) 1846, 3871
Zsp2I ATGCAT 2 cut(s) 811, 2166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.