AT1G16520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012031
Physical Location & Seq
Forward (+)
5648636 .. 5651167
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16520.1

Sequence Viewer

Length: 978 bp
ATGGGAGACGACCAGCTAGATTTCGAGCTCCCAGAAGAGGTTTTGTCGGTGATTCCGATGGACCCTTTTGAGCAGCTGGATCTCGCGAGGAAGATTACATCAATGGCGATAGCTTCAAGGGTGTCGAATCTGGACTCTGAGGTGGTTGAATTGAGACAGAAGCTTCTGGGTAAGGAAAGTGTTGTCCGTGAGCTTGAGGAGAAAGCGTCTCGCCTGGAGAGAGACTGCCGCGAGGCTGATTCGAGATTGAAGGTCGTTCTCGAAGATAATATGAACCTGACAAAGGAGAAGGATTCACTGGCAATGACTGTAACGAAACTCACCCGTGATTTGGCTAAGCTGGAGACATTCAAGCGGCAGTTAATCAAATCCCTGAGCGATGAGAGTGGTCCGCAAACAGAACCTGTTGATATTAGGACATGCGACCAGCCAGGATCATATCCAGGCAAAGATGGAAGGATCAATGCACACTCAATTAAACAAGCTTACAGTGGATCTACAGACACGAACAATCCAGTTGTTGAAGCTTCAAAATACACTGGAAACAAATTTTCAATGACATCATACATATCTCCACGTCTTACCCCAACCGCAACACCAAAGATCATCTCCACAAGTGTGTCTCCTCGAGGCTATTCTGCAGCAGGTTCTCCTAAAAGAACATCAGGTGCAGTTTCTCCCACAAAGGCAACACTCTGGTACCCGTCAAGTCAGCAATCATCGGCTGCGAATTCTCCTCCTCGCAACCGCACACTCCCAGCTCGTACACCGCGGATGGATGGTAAAGAATTCTTCCGGCAAGCCAGGAGCCGATTATCCTATGAACAATTCAGTTCCTTCTTAGCTAACATCAAAGAGCTCAACGCCCAGAAGCAAACCCGAGAGGAAACTCTGAGGAAAGCAGATGAGATATTTGGGGAAGAGAACAAAGATCTTTATTTATCTTTCCAAGGTCTCCTTAACAGAAACATGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

36.43

Weight (kDa)

8.87

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 257 - 323 1.5e-35 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 627
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 699
AccB1I GGYRCC 1 cut(s) 699
AccII CGCG 3 cut(s) 86, 231, 772
AciI CCGC 7 cut(s) 229, 355, 392, 591, 748, 770, 772
AclWI GGATC 4 cut(s) 87, 442, 467, 502
AcsI RAATTY 3 cut(s) 548, 730, 788
AfaI GTAC 2 cut(s) 701, 766
AfiI CCNNNNNNNGG 3 cut(s) 37, 283, 331
AgsI TTSAA 7 cut(s) 117, 149, 250, 352, 524, 531, 555
AjiI CACGTC 1 cut(s) 578
AjnI CCWGG 4 cut(s) 213, 430, 442, 803
AleI CACNNNNGTG 1 cut(s) 617
Alw21I GWGCWC 2 cut(s) 30, 861
Alw26I GTCTC 6 cut(s) 148, 213, 216, 338, 627, 959
AlwI GGATC 4 cut(s) 87, 442, 467, 502
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 2 cut(s) 627, 879
ApeKI GCWGC 3 cut(s) 73, 641, 725
ApoI RAATTY 3 cut(s) 548, 730, 788
Asp718I GGTACC 1 cut(s) 699
AspS9I GGNCC 2 cut(s) 61, 389
AsuHPI GGTGA 2 cut(s) 61, 313
AvaI CYCGRG 2 cut(s) 627, 879
AvaII GGWCC 2 cut(s) 61, 389
BaeI ACNNNNGTAYC 2 cut(s) 683, 716
BanI GGYRCC 1 cut(s) 699
BanII GRGCYC 2 cut(s) 30, 861
Bbv12I GWGCWC 2 cut(s) 30, 861
BbvI GCAGC 3 cut(s) 85, 653, 712
BccI CCATC 4 cut(s) 52, 446, 769, 773
BciT130I CCWGG 4 cut(s) 215, 432, 444, 805
BcoDI GTCTC 6 cut(s) 148, 213, 216, 338, 627, 959
BfaI CTAG 1 cut(s) 17
BfmI CTRYAG 2 cut(s) 498, 639
BfuAI ACCTGC 1 cut(s) 635
BglII AGATCT 1 cut(s) 931
BisI GCNGC 5 cut(s) 74, 229, 356, 642, 726
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 5 cut(s) 75, 230, 357, 643, 727
Bme1390I CCNGG 4 cut(s) 215, 432, 444, 805
Bme18I GGWCC 2 cut(s) 61, 389
BmeT110I CYCGRG 2 cut(s) 627, 879
BmgBI CACGTC 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 61, 389
BmiI GGNNCC 3 cut(s) 63, 701, 809
BmrFI CCNGG 4 cut(s) 215, 432, 444, 805
BpmI CTGGAG 2 cut(s) 236, 362
Bpu10I CCTNAGC 1 cut(s) 374
Bpu1102I GCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 215
BsaI GGTCTC 1 cut(s) 959
BsaJI CCNNGG 2 cut(s) 770, 949
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 283, 331
Bse1I ACTGG 3 cut(s) 303, 515, 544
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 4 cut(s) 215, 432, 444, 805
BseDI CCNNGG 2 cut(s) 770, 949
BseGI GGATG 2 cut(s) 780, 784
BseLI CCNNNNNNNGG 3 cut(s) 37, 283, 331
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 129, 365, 884
BseNI ACTGG 3 cut(s) 303, 515, 544
BseRI GAGGAG 4 cut(s) 212, 615, 726, 729
BseXI GCAGC 3 cut(s) 85, 653, 712
BseYI CCCAGC 1 cut(s) 757
BsgI GTGCAG 1 cut(s) 690
Bsh1236I CGCG 3 cut(s) 86, 231, 772
BshNI GGYRCC 1 cut(s) 699
BsiHKAI GWGCWC 2 cut(s) 30, 861
BsiHKCI CYCGRG 2 cut(s) 627, 879
BsiSI CCGG 1 cut(s) 796
BslI CCNNNNNNNGG 3 cut(s) 37, 283, 331
BsmAI GTCTC 6 cut(s) 148, 213, 216, 338, 627, 959
BsmBI CGTCTC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 959
BsoBI CYCGRG 2 cut(s) 627, 879
Bsp1286I GDGCHC 2 cut(s) 30, 861
Bsp143I GATC 6 cut(s) 79, 434, 459, 494, 603, 931
Bsp1720I GCTNAGC 1 cut(s) 336
Bsp68I TCGCGA 1 cut(s) 86
BspACI CCGC 7 cut(s) 229, 355, 392, 591, 748, 770, 772
BspCNI CTCAG 3 cut(s) 130, 366, 885
BspFNI CGCG 3 cut(s) 86, 231, 772
BspLI GGNNCC 3 cut(s) 63, 701, 809
BspMAI CTGCAG 1 cut(s) 643
BspMI ACCTGC 1 cut(s) 635
BspPI GGATC 4 cut(s) 87, 442, 467, 502
BspT107I GGYRCC 1 cut(s) 699
BspTNI GGTCTC 1 cut(s) 959
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 3 cut(s) 303, 515, 544
BssECI CCNNGG 2 cut(s) 770, 949
BssMI GATC 6 cut(s) 79, 434, 459, 494, 603, 931
BssT1I CCWWGG 1 cut(s) 949
Bst2UI CCWGG 4 cut(s) 215, 432, 444, 805
Bst4CI ACNGT 2 cut(s) 310, 491
Bst6I CTCTTC 2 cut(s) 30, 915
BstC8I GCNNGC 1 cut(s) 801
BstDEI CTNAG 5 cut(s) 138, 336, 374, 841, 893
BstDSI CCRYGG 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 780, 784
BstFNI CGCG 3 cut(s) 86, 231, 772
BstKTI GATC 6 cut(s) 82, 437, 462, 497, 606, 934
BstMAI GTCTC 6 cut(s) 148, 213, 216, 338, 627, 959
BstMBI GATC 6 cut(s) 79, 434, 459, 494, 603, 931
BstNI CCWGG 4 cut(s) 215, 432, 444, 805
BstNSI RCATGY 2 cut(s) 423, 973
BstSCI CCNGG 4 cut(s) 213, 430, 442, 803
BstSFI CTRYAG 2 cut(s) 498, 639
BstUI CGCG 3 cut(s) 86, 231, 772
BstV1I GCAGC 3 cut(s) 85, 653, 712
BstX2I RGATCY 3 cut(s) 79, 494, 931
BstYI RGATCY 3 cut(s) 79, 494, 931
BtgI CCRYGG 1 cut(s) 770
BtgZI GCGATG 1 cut(s) 393
BtrI CACGTC 1 cut(s) 578
BtsCI GGATG 2 cut(s) 780, 784
BtsIMutI CAGTG 3 cut(s) 296, 496, 537
BtuMI TCGCGA 1 cut(s) 86
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 801
CaiI CAGNNNCTG 1 cut(s) 404
Cfr13I GGNCC 2 cut(s) 61, 389
Cfr42I CCGCGG 1 cut(s) 773
CseI GACGC 1 cut(s) 195
Csp6I GTAC 2 cut(s) 700, 765
CviAII CATG 2 cut(s) 420, 970
CviQI GTAC 2 cut(s) 700, 765
DdeI CTNAG 5 cut(s) 138, 336, 374, 841, 893
DpnI GATC 6 cut(s) 81, 436, 461, 496, 605, 933
DpnII GATC 6 cut(s) 79, 434, 459, 494, 603, 931
Eam1104I CTCTTC 2 cut(s) 30, 915
EarI CTCTTC 2 cut(s) 30, 915
Ecl136II GAGCTC 2 cut(s) 28, 859
Eco130I CCWWGG 1 cut(s) 949
Eco24I GRGCYC 2 cut(s) 30, 861
Eco31I GGTCTC 1 cut(s) 959
Eco47I GGWCC 2 cut(s) 61, 389
Eco53kI GAGCTC 2 cut(s) 28, 859
Eco88I CYCGRG 2 cut(s) 627, 879
EcoICRI GAGCTC 2 cut(s) 28, 859
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoRI GAATTC 2 cut(s) 730, 788
EcoRII CCWGG 4 cut(s) 213, 430, 442, 803
EcoT14I CCWWGG 1 cut(s) 949
EcoT38I GRGCYC 2 cut(s) 30, 861
ErhI CCWWGG 1 cut(s) 949
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 2 cut(s) 423, 973
FaiI YATR 7 cut(s) 272, 421, 439, 565, 569, 822, 971
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FatI CATG 2 cut(s) 419, 969
Fnu4HI GCNGC 5 cut(s) 74, 229, 356, 642, 726
FokI GGATG 2 cut(s) 787, 791
FriOI GRGCYC 2 cut(s) 30, 861
Fsp4HI GCNGC 5 cut(s) 74, 229, 356, 642, 726
FspBI CTAG 1 cut(s) 17
GluI GCNGC 5 cut(s) 74, 229, 356, 642, 726
GsaI CCCAGC 1 cut(s) 761
GsuI CTGGAG 2 cut(s) 236, 362
HapII CCGG 1 cut(s) 796
HgaI GACGC 1 cut(s) 195
Hin1II CATG 2 cut(s) 423, 973
HindIII AAGCTT 3 cut(s) 161, 483, 525
HinfI GANTC 5 cut(s) 52, 127, 134, 239, 293
HpaII CCGG 1 cut(s) 796
HphI GGTGA 2 cut(s) 61, 313
Hpy166II GTNNAC 1 cut(s) 767
Hpy188I TCNGA 3 cut(s) 57, 139, 894
Hpy188III TCNNGA 4 cut(s) 85, 131, 243, 260
Hpy8I GTNNAC 1 cut(s) 767
HpyAV CCTTC 4 cut(s) 244, 283, 450, 847
HpyCH4III ACNGT 2 cut(s) 310, 491
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 3 cut(s) 467, 641, 671
HpyF3I CTNAG 5 cut(s) 138, 336, 374, 841, 893
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 2 cut(s) 423, 973
KpnI GGTACC 1 cut(s) 703
KspI CCGCGG 1 cut(s) 773
Kzo9I GATC 6 cut(s) 79, 434, 459, 494, 603, 931
LmnI GCTCC 2 cut(s) 33, 807
Lsp1109I GCAGC 3 cut(s) 85, 653, 712
MaeI CTAG 1 cut(s) 17
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 1 cut(s) 310
MalI GATC 6 cut(s) 81, 436, 461, 496, 605, 933
MboI GATC 6 cut(s) 79, 434, 459, 494, 603, 931
MboII GAAGA 5 cut(s) 47, 103, 275, 784, 932
MflI RGATCY 3 cut(s) 79, 494, 931
MhlI GDGCHC 2 cut(s) 30, 861
MluCI AATT 6 cut(s) 149, 474, 548, 730, 788, 827
MlyI GAGTC 1 cut(s) 128
MseI TTAA 4 cut(s) 362, 477, 960, 976
MslI CAYNNNNRTG 1 cut(s) 617
MspA1I CMGCKG 2 cut(s) 76, 772
MspI CCGG 1 cut(s) 796
MspR9I CCNGG 4 cut(s) 215, 432, 444, 805
MvaI CCWGG 4 cut(s) 215, 432, 444, 805
MvnI CGCG 3 cut(s) 86, 231, 772
NdeII GATC 6 cut(s) 79, 434, 459, 494, 603, 931
NlaIII CATG 2 cut(s) 423, 973
NlaIV GGNNCC 3 cut(s) 63, 701, 809
NruI TCGCGA 1 cut(s) 86
NspI RCATGY 2 cut(s) 423, 973
OliI CACNNNNGTG 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PfeI GAWTC 4 cut(s) 52, 127, 239, 293
PkrI GCNGC 5 cut(s) 75, 230, 357, 643, 727
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Psp124BI GAGCTC 2 cut(s) 30, 861
Psp6I CCWGG 4 cut(s) 213, 430, 442, 803
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 4 cut(s) 213, 430, 442, 803
PspN4I GGNNCC 3 cut(s) 63, 701, 809
PspPI GGNCC 2 cut(s) 61, 389
PspXI VCTCGAGB 1 cut(s) 627
PstI CTGCAG 1 cut(s) 643
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 3 cut(s) 79, 494, 931
PvuII CAGCTG 1 cut(s) 76
RruI TCGCGA 1 cut(s) 86
RsaI GTAC 2 cut(s) 701, 766
RsaNI GTAC 2 cut(s) 700, 765
RseI CAYNNNNRTG 1 cut(s) 617
SacI GAGCTC 2 cut(s) 30, 861
SacII CCGCGG 1 cut(s) 773
SaqAI TTAA 4 cut(s) 362, 477, 960, 976
SatI GCNGC 5 cut(s) 74, 229, 356, 642, 726
Sau3AI GATC 6 cut(s) 79, 434, 459, 494, 603, 931
Sau96I GGNCC 2 cut(s) 61, 389
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 4 cut(s) 215, 432, 444, 805
SduI GDGCHC 2 cut(s) 30, 861
SfcI CTRYAG 2 cut(s) 498, 639
Sfr274I CTCGAG 1 cut(s) 627
Sfr303I CCGCGG 1 cut(s) 773
SgrBI CCGCGG 1 cut(s) 773
SinI GGWCC 2 cut(s) 61, 389
SlaI CTCGAG 1 cut(s) 627
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 194, 627
SmoI CTYRAG 2 cut(s) 194, 627
Sse9I AATT 6 cut(s) 149, 474, 548, 730, 788, 827
SsiI CCGC 7 cut(s) 229, 355, 392, 591, 748, 770, 772
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 2 cut(s) 30, 861
StyD4I CCNGG 4 cut(s) 213, 430, 442, 803
StyI CCWWGG 1 cut(s) 949
TaaI ACNGT 2 cut(s) 310, 491
TaiI ACGT 1 cut(s) 580
TaqI TCGA 5 cut(s) 24, 125, 242, 261, 628
TasI AATT 6 cut(s) 149, 474, 548, 730, 788, 827
TauI GCSGC 2 cut(s) 231, 358
TfiI GAWTC 4 cut(s) 52, 127, 239, 293
Tru1I TTAA 4 cut(s) 362, 477, 960, 976
Tru9I TTAA 4 cut(s) 362, 477, 960, 976
TscAI CASTG 3 cut(s) 303, 496, 544
TseI GCWGC 3 cut(s) 73, 641, 725
TspDTI ATGAA 2 cut(s) 287, 837
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 3 cut(s) 303, 496, 544
VpaK11BI GGWCC 2 cut(s) 61, 389
XagI CCTNNNNNAGG 1 cut(s) 281
XapI RAATTY 3 cut(s) 548, 730, 788
XceI RCATGY 2 cut(s) 423, 973
XhoI CTCGAG 1 cut(s) 627
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.