MD10G1203900.v1.1

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012031
Physical Location & Seq
Reverse (-)
30298939 .. 30302682
3744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1203900.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGTCGCAGGGCAGTGGAACCGTTGTCGTCGTCGGCGGTGGTGGTGGGGGTGACGGGGGGCTGGAGTTTCAGCTGCCGGACGAGATCTTATCGGTTATACCGAGCGACCCGTACGACCAGCTGGATCTGGCGCGGAAGATTACGTCCATGGCCATAGCGTCTAGGGTTTCGAAGCTGGAGTCTGAGATGGGGAAAATGAGGCAGAAGCTCCAGGACAAAGATAGGGTTGTGTTTGAGCTGGAGGAAAGGGTTTCCCGCCTCCAGCACGCCAACCACGAGGCTGAGTCTCGCCTGAGAATTGCCCTGGATGAGAATTTGAAGGTTTCCAAGGAACGAGATTCATTGGCATTGACTACAAAAAAGCTCAGCCGTGATTTAGCAAAGTTGGAGACATTTAAGAGACAACTAATGCAGTCACTGAATGATGATAACGGATCCCCAGCTGAAACTGTTGATATTGGCACAGTTCCTAAGGCATATCCTGAAAAGGATGAGGAGACAAATGTCTACACTGGACAATATTCCTCAAGTGGTTCCACAGATAAGGGAGCAACTGATGAAGCCTTGAAGAATTCTGGTCAAGGATTTTCTCTGACTCCATATATCACTCCGCGGCTTACTCCAACTGGAACTCCAAAGATTATTTCCACAAGTGGGTCACCTAGAGGGTATTCTGCCATTTCTTCTCCTCAGCAATCCTCTGGTGCCACATCTCCCCAAAAACCCGCCTATAAAGAACGGAGTTCATTTTCTTCATGGTACTCGTCAAGCCAGCAGTCATCAGCAGCAAACTCTCCACCTCGTGGACGTTCACATTCAGGGCGTACTCCTAGAATTGACGGAAAAGAGTTCTTTCGTCAAGCCAGGAGCCGTCTATCATATGAGCAGTTCAGTGCATTTCTGGCTAACATCAAGGAACTAAATGCTCATAAGCAAACCAGAGAGGAAACTTTAAGGAAAGCAGAAGAAATATTCGGGACTGATAACAAAGATCTTTATTTATCATTCCAAGGACTGCTTAACCGCAATATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

346

Amino Acids

37.96

Weight (kDa)

8.81

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 277 - 343 8.5e-36 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 704
AccB7I CCANNNNNTGG 1 cut(s) 803
AccI GTMKAC 1 cut(s) 507
AccII CGCG 2 cut(s) 133, 613
AciI CCGC 7 cut(s) 36, 133, 256, 611, 613, 726, 1024
AclWI GGATC 3 cut(s) 132, 429, 442
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 2 cut(s) 313, 571
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 3 cut(s) 113, 761, 826
AfiI CCNNNNNNNGG 2 cut(s) 654, 803
AgsI TTSAA 2 cut(s) 319, 568
AjnI CCWGG 3 cut(s) 210, 303, 863
AjuI GAANNNNNNNTTGG 2 cut(s) 628, 660
AluBI AGCT 7 cut(s) 73, 121, 175, 208, 238, 364, 443
AluI AGCT 7 cut(s) 73, 121, 175, 208, 238, 364, 443
Alw26I GTCTC 4 cut(s) 291, 383, 394, 491
AlwI GGATC 3 cut(s) 132, 429, 442
AlwNI CAGNNNCTG 1 cut(s) 418
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 73, 785
ApoI RAATTY 2 cut(s) 313, 571
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AspLEI GCGC 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 62, 651
AsuII TTCGAA 1 cut(s) 170
AxyI CCTNAGG 1 cut(s) 471
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 1 cut(s) 434
BanI GGYRCC 1 cut(s) 704
BauI CACGAG 2 cut(s) 275, 801
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 2 cut(s) 60, 797
BccI CCATC 1 cut(s) 181
BceAI ACGGC 2 cut(s) 354, 855
BciT130I CCWGG 3 cut(s) 212, 305, 865
BcoDI GTCTC 4 cut(s) 291, 383, 394, 491
BfaI CTAG 4 cut(s) 162, 663, 831, 1036
BglII AGATCT 2 cut(s) 84, 991
BisI GCNGC 3 cut(s) 74, 614, 786
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 3 cut(s) 75, 615, 787
Bme1390I CCNGG 3 cut(s) 212, 305, 865
BmiI GGNNCC 5 cut(s) 19, 436, 535, 706, 869
BmrFI CCNGG 3 cut(s) 212, 305, 865
BoxI GACNNNNGTC 1 cut(s) 503
BpmI CTGGAG 5 cut(s) 83, 194, 197, 245, 260
Bpu10I CCTNAGC 1 cut(s) 690
Bpu1102I GCTNAGC 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 511
BsaJI CCNNGG 5 cut(s) 147, 303, 327, 611, 1009
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 654, 803
Bse1I ACTGG 2 cut(s) 517, 631
Bse21I CCTNAGG 1 cut(s) 471
BseBI CCWGG 3 cut(s) 212, 305, 865
BseDI CCNNGG 5 cut(s) 147, 303, 327, 611, 1009
BseGI GGATG 2 cut(s) 313, 496
BseLI CCNNNNNNNGG 2 cut(s) 654, 803
BseMII CTCAG 5 cut(s) 174, 273, 284, 379, 704
BseNI ACTGG 2 cut(s) 517, 631
BseRI GAGGAG 2 cut(s) 509, 678
BseXI GCAGC 2 cut(s) 60, 797
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 2 cut(s) 133, 613
BshFI GGCC 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 704
BsiSI CCGG 1 cut(s) 77
BsiWI CGTACG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 991
BslI CCNNNNNNNGG 2 cut(s) 654, 803
BsmAI GTCTC 4 cut(s) 291, 383, 394, 491
BsmFI GGGAC 1 cut(s) 991
BsnI GGCC 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 170
Bsp143I GATC 4 cut(s) 84, 124, 434, 991
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 7 cut(s) 36, 133, 256, 611, 613, 726, 1024
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 5 cut(s) 175, 274, 285, 378, 703
BspFNI CGCG 2 cut(s) 133, 613
BspLI GGNNCC 5 cut(s) 19, 436, 535, 706, 869
BspPI GGATC 3 cut(s) 132, 429, 442
BspT104I TTCGAA 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 704
BsrI ACTGG 2 cut(s) 517, 631
BssECI CCNNGG 5 cut(s) 147, 303, 327, 611, 1009
BssMI GATC 4 cut(s) 84, 124, 434, 991
BssSI CACGAG 2 cut(s) 275, 801
BssT1I CCWWGG 3 cut(s) 147, 327, 1009
Bst2BI CACGAG 2 cut(s) 275, 801
Bst2UI CCWGG 3 cut(s) 212, 305, 865
Bst4CI ACNGT 3 cut(s) 22, 451, 466
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 267, 773
BstDEI CTNAG 6 cut(s) 183, 282, 293, 365, 471, 690
BstDSI CCRYGG 2 cut(s) 147, 611
BstEII GGTNACC 1 cut(s) 657
BstF5I GGATG 2 cut(s) 313, 496
BstFNI CGCG 2 cut(s) 133, 613
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 4 cut(s) 87, 127, 437, 994
BstMAI GTCTC 4 cut(s) 291, 383, 394, 491
BstMBI GATC 4 cut(s) 84, 124, 434, 991
BstMWI GCNNNNNNNGC 1 cut(s) 902
BstNI CCWGG 3 cut(s) 212, 305, 865
BstPAI GACNNNNGTC 1 cut(s) 503
BstPI GGTNACC 1 cut(s) 657
BstSCI CCNGG 3 cut(s) 210, 303, 863
BstUI CGCG 2 cut(s) 133, 613
BstV1I GCAGC 2 cut(s) 60, 797
BstX2I RGATCY 4 cut(s) 84, 124, 434, 991
BstYI RGATCY 4 cut(s) 84, 124, 434, 991
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 2 cut(s) 147, 611
BtsCI GGATG 2 cut(s) 313, 496
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 4 cut(s) 19, 416, 510, 898
Cac8I GCNNGC 2 cut(s) 267, 773
CaiI CAGNNNCTG 1 cut(s) 418
CfoI GCGC 1 cut(s) 133
Cfr42I CCGCGG 1 cut(s) 614
CseI GACGC 1 cut(s) 147
Csp6I GTAC 3 cut(s) 112, 760, 825
CviAII CATG 2 cut(s) 148, 756
CviQI GTAC 3 cut(s) 112, 760, 825
DdeI CTNAG 6 cut(s) 183, 282, 293, 365, 471, 690
DpnI GATC 4 cut(s) 86, 126, 436, 993
DpnII GATC 4 cut(s) 84, 124, 434, 991
DraIII CACNNNGTG 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 147, 327, 1009
Eco81I CCTNAGG 1 cut(s) 471
Eco91I GGTNACC 1 cut(s) 657
EcoO65I GGTNACC 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 571
EcoRII CCWGG 3 cut(s) 210, 303, 863
EcoT14I CCWWGG 3 cut(s) 147, 327, 1009
ErhI CCWWGG 3 cut(s) 147, 327, 1009
FaeI CATG 2 cut(s) 151, 759
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 1 cut(s) 991
FatI CATG 2 cut(s) 147, 755
FauI CCCGC 2 cut(s) 263, 733
FauNDI CATATG 1 cut(s) 880
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 74, 614, 786
FokI GGATG 2 cut(s) 320, 503
Fsp4HI GCNGC 3 cut(s) 74, 614, 786
FspBI CTAG 4 cut(s) 162, 663, 831, 1036
GlaI GCGC 1 cut(s) 132
GluI GCNGC 3 cut(s) 74, 614, 786
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 5 cut(s) 83, 194, 197, 245, 260
HaeIII GGCC 1 cut(s) 152
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 147
HhaI GCGC 1 cut(s) 133
Hin1II CATG 2 cut(s) 151, 759
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HinfI GANTC 4 cut(s) 179, 284, 338, 595
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 62, 651
Hpy166II GTNNAC 3 cut(s) 508, 806, 812
Hpy188I TCNGA 2 cut(s) 184, 594
Hpy188III TCNNGA 2 cut(s) 482, 976
Hpy8I GTNNAC 3 cut(s) 508, 806, 812
Hpy99I CGWCG 2 cut(s) 32, 35
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 3 cut(s) 22, 451, 466
HpyCH4IV ACGT 2 cut(s) 143, 808
HpyCH4V TGCA 2 cut(s) 412, 896
HpyF10VI GCNNNNNNNGC 1 cut(s) 902
HpyF3I CTNAG 6 cut(s) 183, 282, 293, 365, 471, 690
HpySE526I ACGT 2 cut(s) 143, 808
Hsp92II CATG 2 cut(s) 151, 759
HspAI GCGC 1 cut(s) 131
KspI CCGCGG 1 cut(s) 614
Kzo9I GATC 4 cut(s) 84, 124, 434, 991
LmnI GCTCC 3 cut(s) 213, 548, 867
Lsp1109I GCAGC 2 cut(s) 60, 797
MaeI CTAG 4 cut(s) 162, 663, 831, 1036
MaeII ACGT 2 cut(s) 143, 808
MaeIII GTNAC 3 cut(s) 50, 414, 657
MalI GATC 4 cut(s) 86, 126, 436, 993
MboI GATC 4 cut(s) 84, 124, 434, 991
MboII GAAGA 5 cut(s) 148, 580, 675, 744, 977
MflI RGATCY 4 cut(s) 84, 124, 434, 991
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 4 cut(s) 297, 313, 571, 834
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 3 cut(s) 188, 293, 589
MmeI TCCRAC 2 cut(s) 366, 647
Mox20I TGGCCA 1 cut(s) 152
MscI TGGCCA 1 cut(s) 152
MseI TTAA 3 cut(s) 396, 953, 1020
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 4 cut(s) 73, 121, 443, 613
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 3 cut(s) 212, 305, 865
MvaI CCWGG 3 cut(s) 212, 305, 865
MvnI CGCG 2 cut(s) 133, 613
MwoI GCNNNNNNNGC 1 cut(s) 902
NcoI CCATGG 1 cut(s) 147
NdeI CATATG 1 cut(s) 880
NdeII GATC 4 cut(s) 84, 124, 434, 991
NlaIII CATG 2 cut(s) 151, 759
NlaIV GGNNCC 5 cut(s) 19, 436, 535, 706, 869
NmuCI GTSAC 3 cut(s) 50, 414, 657
NspV TTCGAA 1 cut(s) 170
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 273
PfeI GAWTC 1 cut(s) 338
Pfl23II CGTACG 1 cut(s) 111
PflMI CCANNNNNTGG 1 cut(s) 803
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 3 cut(s) 75, 615, 787
PleI GAGTC 3 cut(s) 187, 292, 589
PpsI GAGTC 3 cut(s) 187, 292, 589
PshAI GACNNNNGTC 1 cut(s) 503
Psp6I CCWGG 3 cut(s) 210, 303, 863
PspEI GGTNACC 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 3 cut(s) 210, 303, 863
PspLI CGTACG 1 cut(s) 111
PspN4I GGNNCC 5 cut(s) 19, 436, 535, 706, 869
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 4 cut(s) 84, 124, 434, 991
PvuII CAGCTG 3 cut(s) 73, 121, 443
RsaI GTAC 3 cut(s) 113, 761, 826
RsaNI GTAC 3 cut(s) 112, 760, 825
SacII CCGCGG 1 cut(s) 614
SaqAI TTAA 3 cut(s) 396, 953, 1020
SatI GCNGC 3 cut(s) 74, 614, 786
Sau3AI GATC 4 cut(s) 84, 124, 434, 991
SchI GAGTC 3 cut(s) 188, 293, 589
ScrFI CCNGG 3 cut(s) 212, 305, 865
Sfr303I CCGCGG 1 cut(s) 614
SfuI TTCGAA 1 cut(s) 170
SgrBI CCGCGG 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
Sse9I AATT 4 cut(s) 297, 313, 571, 834
SsiI CCGC 7 cut(s) 36, 133, 256, 611, 613, 726, 1024
SspI AATATT 2 cut(s) 521, 972
SspMI CTAG 4 cut(s) 162, 663, 831, 1036
StyD4I CCNGG 3 cut(s) 210, 303, 863
StyI CCWWGG 3 cut(s) 147, 327, 1009
TaaI ACNGT 3 cut(s) 22, 451, 466
TaiI ACGT 2 cut(s) 146, 811
TaqI TCGA 1 cut(s) 170
TasI AATT 4 cut(s) 297, 313, 571, 834
TauI GCSGC 1 cut(s) 616
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 3 cut(s) 396, 953, 1020
Tru9I TTAA 3 cut(s) 396, 953, 1020
TscAI CASTG 4 cut(s) 19, 423, 517, 898
TseFI GTSAC 3 cut(s) 50, 414, 657
TseI GCWGC 2 cut(s) 73, 785
Tsp45I GTSAC 3 cut(s) 50, 414, 657
TspDTI ATGAA 4 cut(s) 330, 573, 735, 744
TspGWI ACGGA 3 cut(s) 447, 754, 855
TspRI CASTG 4 cut(s) 19, 423, 517, 898
Van91I CCANNNNNTGG 1 cut(s) 803
XapI RAATTY 2 cut(s) 313, 571
XmiI GTMKAC 1 cut(s) 507
XspI CTAG 4 cut(s) 162, 663, 831, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.