Prupe.4G138000_v2.0.a1

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012031
Physical Location & Seq
Forward (+)
7673929 .. 7680706
6778 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G138000.2

Sequence Viewer

Length: 1050 bp
ATGTCGCAGGGCAGTGAAGGCGCAGGAGGTGCTGGTGGTGGTGGTGATGGCGGCGGGCTCGACTTTCAGCTACCGGAGGAGATCTTATCGGTAATACCGACCGATCCATACGATCAGCTGGATCTGGCGAGAAAGATCACATCCATGGCCATAGCCTCCAGAGTTTCCAAGCTCGAGTCGGAGATGGGGAAAATGAGGCAGAAACTCCAGGACAAAGATAGGCTTGTATTTGAGCTTGAGGAAAGGCTCTCGCGCCTCCAACATGCAAACCACGAGGCTGACTCCCGCTTGAGAATTGCCCTGGATGAGAATGTGAAGCTTTCCAAAGAACGAGATTCACTGGCAGTGACTGCAAAAAAGCTCAACCGTGACTTGGCAAAGTTGGAGACATTTAAGAGACAACTAATGCAGTCTCTAAATGATGATAATGCATCCCCAACTGAAACTGTTGATATTGGCACATGTGACCAATCAGTTCCAAAGGCATATCCTGATAAGGATGAGGAGACAAATGGCTACACACTACAATATTCCTCAAGTGGTTCCACAGATAAGGGGAATACAACTGATGAAGCCTTGAAGACTTCCGGGCAAAGATTTTCTGTGACTCCATATATCACACCGCGTCTTACTCCAACTGGAACTCCAAAGATTATTTCCACAAGTGGGTCACCTAGAGGGTATTCTGCTATTTCATCTCCTCTGCAATCCTCTGGTGCCACATCTCCCACAAAACCCCACTATGAAGGACGGACGTCACTGTCTTCATGGTACTCATCAAGCCAGCAGTCATCAGCAGCAAACTCTCCACCTCGCGGACGTCCACTTCCAGGGCGCACTCCTCGAATTGATGGAAAGGAGTTCTTTCGTCAAGCCAGGAGTCGCCTGTCATATGAGCAGTTCAGTGCATTTCTGGCTAACATCAAGGAACTAAATGCTCATAAGCAAACCCGAGAGGAAACTCTAAGGAAAGCGGAAGAGATATTTGGGACTGATAACAAAGATCTTTATTTATCATTCCAAGGATTGCTTAACCGCAACATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

350

Amino Acids

38.43

Weight (kDa)

7.77

Isoelectric Point (pI)

52.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 760
AatII GACGTC 2 cut(s) 758, 823
AccB1I GGYRCC 1 cut(s) 716
AccII CGCG 3 cut(s) 253, 625, 816
AciI CCGC 7 cut(s) 51, 54, 286, 623, 816, 974, 1036
AclWI GGATC 2 cut(s) 98, 129
AcoI YGGCCR 1 cut(s) 147
AcyI GRCGYC 2 cut(s) 755, 820
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 4 cut(s) 373, 666, 815, 830
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 1 cut(s) 580
AjnI CCWGG 4 cut(s) 207, 300, 829, 875
AjuI GAANNNNNNNTTGG 4 cut(s) 640, 672, 969, 1001
AluBI AGCT 6 cut(s) 70, 118, 172, 235, 319, 361
AluI AGCT 6 cut(s) 70, 118, 172, 235, 319, 361
Alw26I GTCTC 4 cut(s) 380, 391, 417, 500
AlwI GGATC 2 cut(s) 98, 129
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 2 cut(s) 173, 951
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 797
AspLEI GCGC 3 cut(s) 23, 255, 837
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 2 cut(s) 56, 663
AvaI CYCGRG 2 cut(s) 173, 951
BalI TGGCCA 1 cut(s) 149
BanI GGYRCC 1 cut(s) 716
BanII GRGCYC 1 cut(s) 60
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 587, 756
BbvI GCAGC 1 cut(s) 809
BccI CCATC 3 cut(s) 41, 178, 845
BciT130I CCWGG 4 cut(s) 209, 302, 831, 877
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 4 cut(s) 380, 391, 417, 500
BfaI CTAG 2 cut(s) 675, 1048
BglII AGATCT 2 cut(s) 81, 1003
BisI GCNGC 2 cut(s) 52, 798
BlsI GCNGC 2 cut(s) 53, 799
Bme1390I CCNGG 5 cut(s) 209, 302, 589, 831, 877
BmeT110I CYCGRG 2 cut(s) 173, 951
BmiI GGNNCC 2 cut(s) 544, 718
BmrFI CCNGG 5 cut(s) 209, 302, 589, 831, 877
BmsI GCATC 1 cut(s) 440
BoxI GACNNNNGTC 1 cut(s) 754
BpiI GAAGAC 2 cut(s) 587, 756
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BpmI CTGGAG 2 cut(s) 142, 191
BpuEI CTTGAG 3 cut(s) 257, 310, 520
BpuMI CCSGG 1 cut(s) 589
BsaHI GRCGYC 2 cut(s) 755, 820
BsaJI CCNNGG 4 cut(s) 144, 300, 830, 1021
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 4 cut(s) 373, 666, 815, 830
Bse1I ACTGG 2 cut(s) 345, 643
BseBI CCWGG 4 cut(s) 209, 302, 831, 877
BseDI CCNNGG 4 cut(s) 144, 300, 830, 1021
BseGI GGATG 4 cut(s) 140, 310, 431, 505
BseLI CCNNNNNNNGG 4 cut(s) 373, 666, 815, 830
BseNI ACTGG 2 cut(s) 345, 643
BseRI GAGGAG 4 cut(s) 92, 518, 690, 831
BseXI GCAGC 1 cut(s) 809
Bsh1236I CGCG 3 cut(s) 253, 625, 816
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 149
BshNI GGYRCC 1 cut(s) 716
BsiEI CGRYCG 1 cut(s) 102
BsiHKCI CYCGRG 2 cut(s) 173, 951
BsiSI CCGG 2 cut(s) 74, 588
BslFI GGGAC 1 cut(s) 1003
BslI CCNNNNNNNGG 4 cut(s) 373, 666, 815, 830
BsmAI GTCTC 4 cut(s) 380, 391, 417, 500
BsmFI GGGAC 1 cut(s) 1003
BsnI GGCC 1 cut(s) 149
BsoBI CYCGRG 2 cut(s) 173, 951
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 7 cut(s) 51, 54, 286, 623, 816, 974, 1036
BspANI GGCC 1 cut(s) 149
BspFNI CGCG 3 cut(s) 253, 625, 816
BspLI GGNNCC 2 cut(s) 544, 718
BspPI GGATC 2 cut(s) 98, 129
BspT107I GGYRCC 1 cut(s) 716
BsrI ACTGG 2 cut(s) 345, 643
BssECI CCNNGG 4 cut(s) 144, 300, 830, 1021
BssMI GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
BssNI GRCGYC 2 cut(s) 755, 820
BssSI CACGAG 1 cut(s) 272
BssT1I CCWWGG 2 cut(s) 144, 1021
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 4 cut(s) 209, 302, 831, 877
Bst4CI ACNGT 3 cut(s) 368, 448, 762
Bst6I CTCTTC 1 cut(s) 972
BstACI GRCGYC 2 cut(s) 755, 820
BstAPI GCANNNNNTGC 2 cut(s) 29, 350
BstC8I GCNNGC 2 cut(s) 56, 785
BstDEI CTNAG 1 cut(s) 965
BstDSI CCRYGG 1 cut(s) 144
BstEII GGTNACC 1 cut(s) 669
BstF5I GGATG 4 cut(s) 140, 310, 431, 505
BstFNI CGCG 3 cut(s) 253, 625, 816
BstHHI GCGC 3 cut(s) 23, 255, 837
BstKTI GATC 6 cut(s) 84, 106, 115, 124, 138, 1006
BstMAI GTCTC 4 cut(s) 380, 391, 417, 500
BstMBI GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 4 cut(s) 18, 29, 350, 914
BstNI CCWGG 4 cut(s) 209, 302, 831, 877
BstNSI RCATGY 2 cut(s) 266, 465
BstPAI GACNNNNGTC 1 cut(s) 754
BstPI GGTNACC 1 cut(s) 669
BstSCI CCNGG 5 cut(s) 207, 300, 587, 829, 875
BstUI CGCG 3 cut(s) 253, 625, 816
BstV1I GCAGC 1 cut(s) 809
BstV2I GAAGAC 2 cut(s) 587, 756
BstX2I RGATCY 3 cut(s) 81, 121, 1003
BstYI RGATCY 3 cut(s) 81, 121, 1003
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 4 cut(s) 140, 310, 431, 505
BtsI GCAGTG 2 cut(s) 19, 351
BtsIMutI CAGTG 5 cut(s) 19, 338, 351, 758, 910
Cac8I GCNNGC 2 cut(s) 56, 785
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 3 cut(s) 23, 255, 837
CseI GACGC 1 cut(s) 614
Csp6I GTAC 1 cut(s) 772
CviAII CATG 4 cut(s) 145, 263, 462, 768
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 1 cut(s) 965
DpnI GATC 6 cut(s) 83, 105, 114, 123, 137, 1005
DpnII GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
DrdI GACNNNNNNGTC 1 cut(s) 760
DseDI GACNNNNNNGTC 1 cut(s) 760
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Eco130I CCWWGG 2 cut(s) 144, 1021
Eco24I GRGCYC 1 cut(s) 60
Eco88I CYCGRG 2 cut(s) 173, 951
Eco91I GGTNACC 1 cut(s) 669
EcoO65I GGTNACC 1 cut(s) 669
EcoRII CCWGG 4 cut(s) 207, 300, 829, 875
EcoT14I CCWWGG 2 cut(s) 144, 1021
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 144, 1021
FaeI CATG 4 cut(s) 148, 266, 465, 771
FalI AAGNNNNNCTT 6 cut(s) 207, 239, 848, 880, 1014, 1046
FaqI GGGAC 1 cut(s) 1003
FatI CATG 4 cut(s) 144, 262, 461, 767
FauI CCCGC 2 cut(s) 47, 293
FauNDI CATATG 1 cut(s) 892
Fnu4HI GCNGC 2 cut(s) 52, 798
FokI GGATG 4 cut(s) 127, 317, 418, 512
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 2 cut(s) 52, 798
FspBI CTAG 2 cut(s) 675, 1048
GlaI GCGC 3 cut(s) 22, 254, 836
GluI GCNGC 2 cut(s) 52, 798
GsuI CTGGAG 2 cut(s) 142, 191
HaeIII GGCC 1 cut(s) 149
HapII CCGG 2 cut(s) 74, 588
HgaI GACGC 1 cut(s) 614
HhaI GCGC 3 cut(s) 23, 255, 837
Hin1I GRCGYC 2 cut(s) 755, 820
Hin1II CATG 4 cut(s) 148, 266, 465, 771
Hin6I GCGC 3 cut(s) 21, 253, 835
HinP1I GCGC 3 cut(s) 21, 253, 835
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 5 cut(s) 176, 281, 335, 607, 880
HpaII CCGG 2 cut(s) 74, 588
HphI GGTGA 2 cut(s) 56, 663
Hpy166II GTNNAC 1 cut(s) 824
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 2 cut(s) 159, 491
Hpy8I GTNNAC 1 cut(s) 824
HpyAV CCTTC 2 cut(s) 11, 740
HpyCH4III ACNGT 3 cut(s) 368, 448, 762
HpyCH4IV ACGT 2 cut(s) 755, 820
HpyCH4V TGCA 6 cut(s) 266, 353, 409, 431, 706, 908
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 29, 350, 914
HpyF3I CTNAG 1 cut(s) 965
HpySE526I ACGT 2 cut(s) 755, 820
Hsp92I GRCGYC 2 cut(s) 755, 820
Hsp92II CATG 4 cut(s) 148, 266, 465, 771
HspAI GCGC 3 cut(s) 21, 253, 835
Kzo9I GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
Lsp1109I GCAGC 1 cut(s) 809
LweI GCATC 1 cut(s) 440
MaeI CTAG 2 cut(s) 675, 1048
MaeII ACGT 2 cut(s) 755, 820
MaeIII GTNAC 6 cut(s) 346, 368, 464, 604, 669, 756
MalI GATC 6 cut(s) 83, 105, 114, 123, 137, 1005
MboI GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
MboII GAAGA 3 cut(s) 592, 756, 989
MflI RGATCY 3 cut(s) 81, 121, 1003
MhlI GDGCHC 1 cut(s) 60
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 2 cut(s) 294, 846
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 4 cut(s) 185, 275, 601, 889
MmeI TCCRAC 4 cut(s) 159, 283, 363, 659
Mox20I TGGCCA 1 cut(s) 149
Mph1103I ATGCAT 1 cut(s) 433
MscI TGGCCA 1 cut(s) 149
MseI TTAA 2 cut(s) 393, 1032
MslI CAYNNNNRTG 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 2 cut(s) 74, 588
MspR9I CCNGG 5 cut(s) 209, 302, 589, 831, 877
MvaI CCWGG 4 cut(s) 209, 302, 831, 877
MvnI CGCG 3 cut(s) 253, 625, 816
MwoI GCNNNNNNNGC 4 cut(s) 18, 29, 350, 914
NciI CCSGG 1 cut(s) 589
NcoI CCATGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 892
NdeII GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
NlaIII CATG 4 cut(s) 148, 266, 465, 771
NlaIV GGNNCC 2 cut(s) 544, 718
NmuCI GTSAC 6 cut(s) 346, 368, 464, 604, 669, 756
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 2 cut(s) 266, 465
PaeR7I CTCGAG 1 cut(s) 173
PciI ACATGT 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PfeI GAWTC 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 2 cut(s) 53, 799
PleI GAGTC 4 cut(s) 184, 275, 601, 888
PpsI GAGTC 4 cut(s) 184, 275, 601, 888
PscI ACATGT 1 cut(s) 461
PshAI GACNNNNGTC 1 cut(s) 754
Psp6I CCWGG 4 cut(s) 207, 300, 829, 875
PspEI GGTNACC 1 cut(s) 669
PspGI CCWGG 4 cut(s) 207, 300, 829, 875
PspN4I GGNNCC 2 cut(s) 544, 718
PspXI VCTCGAGB 1 cut(s) 173
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 3 cut(s) 81, 121, 1003
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 2 cut(s) 393, 1032
SatI GCNGC 2 cut(s) 52, 798
Sau3AI GATC 6 cut(s) 81, 103, 112, 121, 135, 1003
SchI GAGTC 4 cut(s) 185, 275, 601, 889
ScrFI CCNGG 5 cut(s) 209, 302, 589, 831, 877
SduI GDGCHC 1 cut(s) 60
SfaNI GCATC 1 cut(s) 440
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 4 cut(s) 173, 236, 289, 535
SmoI CTYRAG 4 cut(s) 173, 236, 289, 535
Sse9I AATT 2 cut(s) 294, 846
SsiI CCGC 7 cut(s) 51, 54, 286, 623, 816, 974, 1036
SspI AATATT 1 cut(s) 530
SspMI CTAG 2 cut(s) 675, 1048
StyD4I CCNGG 5 cut(s) 207, 300, 587, 829, 875
StyI CCWWGG 2 cut(s) 144, 1021
TaaI ACNGT 3 cut(s) 368, 448, 762
TaiI ACGT 2 cut(s) 758, 823
TaqI TCGA 3 cut(s) 60, 174, 844
TaqII GACCGA 1 cut(s) 116
TasI AATT 2 cut(s) 294, 846
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 2 cut(s) 393, 1032
Tru9I TTAA 2 cut(s) 393, 1032
TscAI CASTG 5 cut(s) 19, 345, 351, 765, 910
TseFI GTSAC 6 cut(s) 346, 368, 464, 604, 669, 756
TseI GCWGC 1 cut(s) 797
Tsp45I GTSAC 6 cut(s) 346, 368, 464, 604, 669, 756
TspDTI ATGAA 4 cut(s) 585, 684, 756, 759
TspGWI ACGGA 1 cut(s) 766
TspRI CASTG 5 cut(s) 19, 345, 351, 765, 910
XceI RCATGY 2 cut(s) 266, 465
XhoI CTCGAG 1 cut(s) 173
XspI CTAG 2 cut(s) 675, 1048
ZraI GACGTC 2 cut(s) 756, 821
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.