Rw5G016670

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012031
Physical Location & Seq
Forward (+)
21159579 .. 21162994
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016670.1

Sequence Viewer

Length: 1029 bp
ATGTCGCAGGGAAGCGGAAGCGTCGGCGGCGGAGGGGGGATGGATTTTCAGTTGCCGGACGAGATCTTGGCGGTGATTCCGAGCGACCCGTACGAGCAGCTGGATCTGGCGCGGAAGATCACGTCGATGGCGATAGCGGCTAGGGTTTCGAATCTGGAGGCGGAGGTCGGGAGGCTGAGGCAGAAGCTGAGCGAGAAAGATAGGGTTATTTCTAACCTCGAGGAAAGAGCCTCGCGCCTCCAGCATGTTAACCACGACGCCGATTCGCGGTTGAGAATTGCGCTGGAGGAGAATATGAGGCTGTCTAAAGAACGAGATTCAGTGGCAATGACCGCTAAGAAGCTTAGCCGTGACTTGGCAAAGTTGGAAACATTTAAGAGACAACTGATGCAGTCTCTAAATGATGATAATTCATCTCCAGCCAAAACTGTTGATATTGGCACCTGTGATCAATCGGTTCCAAAGGCATATCCAGATAAGGATGAGGAGACAAATGGCTACTCAGCCCAATATTCGTCAACTGGTTCTACTGATATGGGGAACACAACTGATGAAGCCTTGAGGAATGCTGGGCAAAAGTTTTCGTTGACTCCCTATATCACACCTCGGCTTACTCCAACTGGAACTCCAAAGATCTTTTCGACAAGTGGGTCACCTAGAGCATATTCTACCATCCCATCTCCTCAGCAGACCTCTGGTGCCACATCTCCCACAAAATCATATGATGGACGGAATTCAGTGTCTTCATGGTACCCATCAAGCCAGCAGTCGTCAGCAGCAAACTCTCCACCTCGTGGACGCCCACTGTCAGGGCGTACTCCTCGAATTGATGGAAAGGAGTTCTTTCGTCAAGCCAGGAGCCGCCTATCATATGAGCAGTTCAGTGCATTTCTGGCTAACATCAAGGAGCTAAATGCTCAAAAGCAAACCCGGGAGGAAACTCTAAGGAAAGCAGAGGAGATCTTTGGAACAGATAACAAAGATCTATATTTATCATTTCAAGGATTGCTTAGCCGCAACATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

37.73

Weight (kDa)

8.98

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 274 - 340 2.3e-35 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 649
AbsI CCTCGAGG 1 cut(s) 218
Acc65I GGTACC 1 cut(s) 750
AccB1I GGYRCC 3 cut(s) 440, 698, 750
AccB7I CCANNNNNTGG 1 cut(s) 794
AccII CGCG 3 cut(s) 112, 235, 268
AclWI GGATC 1 cut(s) 111
AcsI RAATTY 1 cut(s) 733
AcyI GRCGYC 2 cut(s) 258, 799
AdeI CACNNNGTG 1 cut(s) 794
AfaI GTAC 3 cut(s) 92, 752, 817
AfiI CCNNNNNNNGG 4 cut(s) 267, 355, 794, 809
AgsI TTSAA 1 cut(s) 1001
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 1 cut(s) 854
AjuI GAANNNNNNNTTGG 2 cut(s) 622, 654
AluBI AGCT 4 cut(s) 100, 187, 343, 910
AluI AGCT 4 cut(s) 100, 187, 343, 910
Alw26I GTCTC 3 cut(s) 373, 399, 482
AlwI GGATC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 2 cut(s) 218, 930
ApeKI GCWGC 2 cut(s) 97, 776
ApoI RAATTY 1 cut(s) 733
Asp718I GGTACC 1 cut(s) 750
AspLEI GCGC 3 cut(s) 112, 237, 283
AsuC2I CCSGG 2 cut(s) 931, 932
AsuHPI GGTGA 2 cut(s) 85, 645
AsuII TTCGAA 1 cut(s) 149
AvaI CYCGRG 2 cut(s) 218, 930
BanI GGYRCC 3 cut(s) 440, 698, 750
BauI CACGAG 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 735
BbvCI CCTCAGC 2 cut(s) 176, 684
BbvI GCAGC 2 cut(s) 109, 788
BccI CCATC 7 cut(s) 34, 121, 680, 685, 719, 763, 824
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 856
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 2 cut(s) 931, 932
BcoDI GTCTC 3 cut(s) 373, 399, 482
BfaI CTAG 3 cut(s) 141, 657, 1027
BglII AGATCT 4 cut(s) 63, 633, 960, 982
BisI GCNGC 6 cut(s) 28, 98, 138, 777, 862, 1015
BlpI GCTNAGC 3 cut(s) 188, 344, 1010
BlsI GCNGC 6 cut(s) 29, 99, 139, 778, 863, 1016
Bme1390I CCNGG 3 cut(s) 856, 931, 932
BmeT110I CYCGRG 2 cut(s) 218, 930
BmgBI CACGTC 1 cut(s) 123
BmiI GGNNCC 5 cut(s) 442, 459, 700, 752, 860
BmrFI CCNGG 3 cut(s) 856, 931, 932
BmsI GCATC 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 4 cut(s) 176, 224, 305, 402
Bpu10I CCTNAGC 2 cut(s) 176, 684
Bpu1102I GCTNAGC 3 cut(s) 188, 344, 1010
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 580
BpuMI CCSGG 2 cut(s) 931, 932
BsaHI GRCGYC 2 cut(s) 258, 799
BsaJI CCNNGG 2 cut(s) 605, 930
BsaXI ACNNNNNCTCC 2 cut(s) 610, 640
Bsc4I CCNNNNNNNGG 4 cut(s) 267, 355, 794, 809
Bse1I ACTGG 2 cut(s) 526, 625
Bse3DI GCAATG 1 cut(s) 333
BseBI CCWGG 1 cut(s) 856
BseDI CCNNGG 2 cut(s) 605, 930
BseGI GGATG 3 cut(s) 45, 487, 672
BseLI CCNNNNNNNGG 4 cut(s) 267, 355, 794, 809
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 4 cut(s) 167, 179, 516, 698
BseNI ACTGG 2 cut(s) 526, 625
BseRI GAGGAG 5 cut(s) 302, 500, 672, 810, 971
BseXI GCAGC 2 cut(s) 109, 788
BseYI CCCAGC 1 cut(s) 569
Bsh1236I CGCG 3 cut(s) 112, 235, 268
BshNI GGYRCC 3 cut(s) 440, 698, 750
BsiHKCI CYCGRG 2 cut(s) 218, 930
BsiSI CCGG 2 cut(s) 56, 931
BsiWI CGTACG 1 cut(s) 90
BslI CCNNNNNNNGG 4 cut(s) 267, 355, 794, 809
BsmAI GTCTC 3 cut(s) 373, 399, 482
BsmI GAATGC 1 cut(s) 571
BsoBI CYCGRG 2 cut(s) 218, 930
Bsp119I TTCGAA 1 cut(s) 149
Bsp143I GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
Bsp1720I GCTNAGC 3 cut(s) 188, 344, 1010
BspCNI CTCAG 4 cut(s) 168, 180, 515, 697
BspFNI CGCG 3 cut(s) 112, 235, 268
BspLI GGNNCC 5 cut(s) 442, 459, 700, 752, 860
BspPI GGATC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 149
BspT107I GGYRCC 3 cut(s) 440, 698, 750
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 2 cut(s) 526, 625
BssECI CCNNGG 2 cut(s) 605, 930
BssMI GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
BssNI GRCGYC 2 cut(s) 258, 799
BssSI CACGAG 1 cut(s) 792
Bst2BI CACGAG 1 cut(s) 792
Bst2UI CCWGG 1 cut(s) 856
Bst4CI ACNGT 2 cut(s) 430, 807
BstACI GRCGYC 2 cut(s) 258, 799
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 764
BstDEI CTNAG 8 cut(s) 176, 188, 336, 344, 502, 684, 944, 1010
BstEII GGTNACC 1 cut(s) 651
BstF5I GGATG 3 cut(s) 45, 487, 672
BstFNI CGCG 3 cut(s) 112, 235, 268
BstHHI GCGC 3 cut(s) 112, 237, 283
BstKTI GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
BstMAI GTCTC 3 cut(s) 373, 399, 482
BstMBI GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
BstMWI GCNNNNNNNGC 5 cut(s) 27, 137, 241, 332, 893
BstNI CCWGG 1 cut(s) 856
BstNSI RCATGY 1 cut(s) 248
BstPI GGTNACC 1 cut(s) 651
BstSCI CCNGG 3 cut(s) 854, 929, 930
BstUI CGCG 3 cut(s) 112, 235, 268
BstV1I GCAGC 2 cut(s) 109, 788
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 5 cut(s) 63, 103, 633, 960, 982
BstYI RGATCY 5 cut(s) 63, 103, 633, 960, 982
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 3 cut(s) 45, 487, 672
BtsIMutI CAGTG 4 cut(s) 327, 744, 803, 889
Cac8I GCNNGC 1 cut(s) 764
CaiI CAGNNNCTG 1 cut(s) 187
CfoI GCGC 3 cut(s) 112, 237, 283
Cfr9I CCCGGG 1 cut(s) 930
CseI GACGC 3 cut(s) 10, 266, 807
Csp6I GTAC 3 cut(s) 91, 751, 816
CviAII CATG 2 cut(s) 245, 747
CviQI GTAC 3 cut(s) 91, 751, 816
DdeI CTNAG 8 cut(s) 176, 188, 336, 344, 502, 684, 944, 1010
DpnI GATC 7 cut(s) 65, 105, 119, 450, 635, 962, 984
DpnII GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
DraIII CACNNNGTG 1 cut(s) 794
DrdI GACNNNNNNGTC 1 cut(s) 649
DseDI GACNNNNNNGTC 1 cut(s) 649
EciI GGCGGA 2 cut(s) 45, 176
Eco88I CYCGRG 2 cut(s) 218, 930
Eco91I GGTNACC 1 cut(s) 651
EcoO65I GGTNACC 1 cut(s) 651
EcoRI GAATTC 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 854
FaeI CATG 2 cut(s) 248, 750
FalI AAGNNNNNCTT 4 cut(s) 827, 859, 993, 1025
FatI CATG 2 cut(s) 244, 746
FauNDI CATATG 2 cut(s) 721, 871
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 6 cut(s) 28, 98, 138, 777, 862, 1015
FokI GGATG 3 cut(s) 52, 494, 659
Fsp4HI GCNGC 6 cut(s) 28, 98, 138, 777, 862, 1015
FspBI CTAG 3 cut(s) 141, 657, 1027
GlaI GCGC 3 cut(s) 111, 236, 282
GluI GCNGC 6 cut(s) 28, 98, 138, 777, 862, 1015
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 4 cut(s) 176, 224, 305, 402
HapII CCGG 2 cut(s) 56, 931
HgaI GACGC 3 cut(s) 10, 266, 807
HhaI GCGC 3 cut(s) 112, 237, 283
Hin1I GRCGYC 2 cut(s) 258, 799
Hin1II CATG 2 cut(s) 248, 750
Hin6I GCGC 3 cut(s) 110, 235, 281
HinP1I GCGC 3 cut(s) 110, 235, 281
HincII GTYRAC 3 cut(s) 250, 519, 588
HindII GTYRAC 3 cut(s) 250, 519, 588
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 5 cut(s) 76, 151, 263, 317, 589
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 2 cut(s) 56, 931
HphI GGTGA 2 cut(s) 85, 645
Hpy166II GTNNAC 4 cut(s) 250, 519, 588, 797
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 3 cut(s) 155, 169, 473
Hpy8I GTNNAC 4 cut(s) 250, 519, 588, 797
Hpy99I CGWCG 3 cut(s) 26, 127, 260
HpyCH4III ACNGT 2 cut(s) 430, 807
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 2 cut(s) 391, 887
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 137, 241, 332, 893
HpyF3I CTNAG 8 cut(s) 176, 188, 336, 344, 502, 684, 944, 1010
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 2 cut(s) 258, 799
Hsp92II CATG 2 cut(s) 248, 750
HspAI GCGC 3 cut(s) 110, 235, 281
KpnI GGTACC 1 cut(s) 754
Ksp22I TGATCA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 250
Kzo9I GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
LmnI GCTCC 2 cut(s) 858, 907
Lsp1109I GCAGC 2 cut(s) 109, 788
LweI GCATC 1 cut(s) 378
MaeI CTAG 3 cut(s) 141, 657, 1027
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 2 cut(s) 350, 651
MalI GATC 7 cut(s) 65, 105, 119, 450, 635, 962, 984
MboI GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
MboII GAAGA 2 cut(s) 127, 735
MflI RGATCY 5 cut(s) 63, 103, 633, 960, 982
MluCI AATT 4 cut(s) 276, 409, 733, 825
MlyI GAGTC 1 cut(s) 583
MmeI TCCRAC 2 cut(s) 345, 641
MseI TTAA 2 cut(s) 249, 375
MslI CAYNNNNRTG 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 2 cut(s) 56, 931
MspR9I CCNGG 3 cut(s) 856, 931, 932
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 1 cut(s) 856
MvnI CGCG 3 cut(s) 112, 235, 268
MwoI GCNNNNNNNGC 5 cut(s) 27, 137, 241, 332, 893
NciI CCSGG 2 cut(s) 931, 932
NdeI CATATG 2 cut(s) 721, 871
NdeII GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
NlaIII CATG 2 cut(s) 248, 750
NlaIV GGNNCC 5 cut(s) 442, 459, 700, 752, 860
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 2 cut(s) 350, 651
NspI RCATGY 1 cut(s) 248
NspV TTCGAA 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 4 cut(s) 76, 151, 263, 317
Pfl23II CGTACG 1 cut(s) 90
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 6 cut(s) 29, 99, 139, 778, 863, 1016
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 854
PspEI GGTNACC 1 cut(s) 651
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 1 cut(s) 854
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 442, 459, 700, 752, 860
PspXI VCTCGAGB 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 5 cut(s) 63, 103, 633, 960, 982
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 3 cut(s) 92, 752, 817
RsaNI GTAC 3 cut(s) 91, 751, 816
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 2 cut(s) 249, 375
SatI GCNGC 6 cut(s) 28, 98, 138, 777, 862, 1015
Sau3AI GATC 7 cut(s) 63, 103, 117, 448, 633, 960, 982
SchI GAGTC 1 cut(s) 583
ScrFI CCNGG 3 cut(s) 856, 931, 932
SfaNI GCATC 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 218
SmaI CCCGGG 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 218, 559
SmoI CTYRAG 2 cut(s) 218, 559
Sse9I AATT 4 cut(s) 276, 409, 733, 825
SspI AATATT 1 cut(s) 512
SspMI CTAG 3 cut(s) 141, 657, 1027
StyD4I CCNGG 3 cut(s) 854, 929, 930
TaaI ACNGT 2 cut(s) 430, 807
TaiI ACGT 1 cut(s) 125
TaqI TCGA 5 cut(s) 125, 149, 219, 641, 823
TasI AATT 4 cut(s) 276, 409, 733, 825
TauI GCSGC 4 cut(s) 30, 140, 864, 1017
TfiI GAWTC 4 cut(s) 76, 151, 263, 317
Tru1I TTAA 2 cut(s) 249, 375
Tru9I TTAA 2 cut(s) 249, 375
TscAI CASTG 4 cut(s) 327, 744, 810, 889
TseFI GTSAC 2 cut(s) 350, 651
TseI GCWGC 2 cut(s) 97, 776
Tsp45I GTSAC 2 cut(s) 350, 651
TspDTI ATGAA 3 cut(s) 402, 567, 735
TspGWI ACGGA 1 cut(s) 745
TspMI CCCGGG 1 cut(s) 930
TspRI CASTG 4 cut(s) 327, 744, 810, 889
Van91I CCANNNNNTGG 1 cut(s) 794
XapI RAATTY 1 cut(s) 733
XceI RCATGY 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 218
XmaI CCCGGG 1 cut(s) 930
XspI CTAG 3 cut(s) 141, 657, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.