Rh5BG181000

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012031
Physical Location & Seq
Forward (+)
19845014 .. 19849131
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG181000.1

Sequence Viewer

Length: 1032 bp
ATGTCGCAGGGAAGCGGAAGCGTCGGCGGCGGAGGAGGGGGGATGGATTTTCAATTGCCGGACGAGATCTTGGCGGTGATTCCGGGGGACCCGTACGAGCAGCTGGATCTGGCGAGGAAGATCACGTCGATGGCGATAGCGGCTAGGGTTTCGAATCTGGAGGCGGAGGTCGGGAGGCTGGGGCAGAAGCTGAGCGAGAAAGATAGGGTTATTTCTGACCTCGAGGAAAGAGCCTCGCGCCTCCAGCATGTTAACCACGACGCCGACTCGCGGTTGAGAATTGCGCTAGAGGAGAATATGAGGCTTTCCAAAGAACGAGATTCAGTGGCAATGACTGCTGAGAAGCTTAGCCGTGACTTGGCAAAGTTGGAAACATTTAAGAGACAACTGATGCAGTCTCTAAACGATGATAATTCATCTCCAGCCAAAACTGTTGATATTGGCACCTGTGATCAATCGGTTCCAAAGGCATATCCAGATAAGGATGAGGAGACAAATGGCTACTCAGCCCAATATTCGTCAACTGGTTCTACTGATATGGGGAACACAACTGATGAAGCCTCGAGGAATGCTGGGCAAAAGTTTTCATTGACTCCCTATATCACACCTCGTCTTACTCCAACTGGAACTCCAAAGATCTTTTCGACAAGTGGGTCACCTAGAGCATATTCTACCATCCCATCTCCTCAGCAGACCTCTGGTGCCACATCTCCCACAAAATCATATGATGGACGGAATTCAGTGTCTTCATGGTACGCATCAAGCCAGCAGTCATCAGCAGCAAACTCTCCACCTCGTGGACGCCCACTGTCAGGGCGTACTCCTCGAATTGATGGAAAGGAGTTCTTTCGTCAAGCCAGGAGCCGCCTATCATATGAGCAGTTCAGTGCATTTCTGGCTAACATCAAGGAGCTAAATGCTCAAAAGCAAACCCGGGAGGAAACTCTAAGGAAAGCAGAGGAGATCTTTGGGACAGATAACAAAGATCTATATTTATCATTTCAAGGATTGCTTAGCCGCAACATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

343

Amino Acids

37.61

Weight (kDa)

6.87

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 275 - 341 2.3e-35 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 652
AbsI CCTCGAGG 2 cut(s) 221, 562
AccB1I GGYRCC 2 cut(s) 443, 701
AccB7I CCANNNNNTGG 1 cut(s) 797
AccII CGCG 2 cut(s) 238, 271
AciI CCGC 9 cut(s) 15, 27, 30, 74, 140, 164, 271, 865, 1018
AclWI GGATC 1 cut(s) 114
AcsI RAATTY 1 cut(s) 736
AcyI GRCGYC 2 cut(s) 261, 802
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 3 cut(s) 95, 755, 820
AfiI CCNNNNNNNGG 4 cut(s) 270, 358, 797, 812
AgsI TTSAA 2 cut(s) 53, 1004
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 1 cut(s) 857
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
AluBI AGCT 4 cut(s) 103, 190, 346, 913
AluI AGCT 4 cut(s) 103, 190, 346, 913
Alw26I GTCTC 3 cut(s) 376, 402, 485
AlwI GGATC 1 cut(s) 114
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 3 cut(s) 221, 562, 933
ApeKI GCWGC 2 cut(s) 100, 779
ApoI RAATTY 1 cut(s) 736
AspLEI GCGC 2 cut(s) 240, 286
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 3 cut(s) 84, 934, 935
AsuHPI GGTGA 2 cut(s) 88, 648
AsuII TTCGAA 1 cut(s) 152
AvaI CYCGRG 3 cut(s) 221, 562, 933
AvaII GGWCC 1 cut(s) 88
BanI GGYRCC 2 cut(s) 443, 701
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 738
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 2 cut(s) 112, 791
BccI CCATC 6 cut(s) 37, 124, 683, 688, 722, 827
BceAI ACGGC 1 cut(s) 336
BciT130I CCWGG 1 cut(s) 859
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 3 cut(s) 84, 934, 935
BcoDI GTCTC 3 cut(s) 376, 402, 485
BfaI CTAG 4 cut(s) 144, 287, 660, 1030
BglII AGATCT 4 cut(s) 66, 636, 963, 985
BisI GCNGC 6 cut(s) 28, 101, 141, 780, 865, 1018
BlpI GCTNAGC 3 cut(s) 191, 347, 1013
BlsI GCNGC 6 cut(s) 29, 102, 142, 781, 866, 1019
Bme1390I CCNGG 4 cut(s) 84, 859, 934, 935
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 3 cut(s) 221, 562, 933
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 6 cut(s) 89, 90, 445, 462, 703, 863
BmrFI CCNGG 4 cut(s) 84, 859, 934, 935
BmsI GCATC 2 cut(s) 381, 767
BpiI GAAGAC 1 cut(s) 738
BpmI CTGGAG 3 cut(s) 179, 227, 405
Bpu10I CCTNAGC 1 cut(s) 687
Bpu1102I GCTNAGC 3 cut(s) 191, 347, 1013
Bpu14I TTCGAA 1 cut(s) 152
BpuMI CCSGG 3 cut(s) 84, 934, 935
BsaHI GRCGYC 2 cut(s) 261, 802
BsaJI CCNNGG 2 cut(s) 83, 933
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 358, 797, 812
Bse1I ACTGG 2 cut(s) 529, 628
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 859
BseDI CCNNGG 2 cut(s) 83, 933
BseGI GGATG 3 cut(s) 48, 490, 675
BseLI CCNNNNNNNGG 4 cut(s) 270, 358, 797, 812
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 4 cut(s) 182, 330, 519, 701
BseNI ACTGG 2 cut(s) 529, 628
BseRI GAGGAG 6 cut(s) 48, 305, 503, 675, 813, 974
BseXI GCAGC 2 cut(s) 112, 791
BseYI CCCAGC 2 cut(s) 178, 572
Bsh1236I CGCG 2 cut(s) 238, 271
BshNI GGYRCC 2 cut(s) 443, 701
BsiHKCI CYCGRG 3 cut(s) 221, 562, 933
BsiSI CCGG 3 cut(s) 59, 83, 934
BsiWI CGTACG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 101, 985
BslI CCNNNNNNNGG 4 cut(s) 270, 358, 797, 812
BsmAI GTCTC 3 cut(s) 376, 402, 485
BsmFI GGGAC 2 cut(s) 101, 985
BsmI GAATGC 1 cut(s) 574
BsoBI CYCGRG 3 cut(s) 221, 562, 933
Bsp119I TTCGAA 1 cut(s) 152
Bsp143I GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
Bsp1720I GCTNAGC 3 cut(s) 191, 347, 1013
BspACI CCGC 9 cut(s) 15, 27, 30, 74, 140, 164, 271, 865, 1018
BspCNI CTCAG 4 cut(s) 183, 331, 518, 700
BspFNI CGCG 2 cut(s) 238, 271
BspLI GGNNCC 6 cut(s) 89, 90, 445, 462, 703, 863
BspPI GGATC 1 cut(s) 114
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 2 cut(s) 443, 701
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 2 cut(s) 529, 628
BssECI CCNNGG 2 cut(s) 83, 933
BssMI GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
BssNI GRCGYC 2 cut(s) 261, 802
BssSI CACGAG 1 cut(s) 795
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 1 cut(s) 859
Bst4CI ACNGT 2 cut(s) 433, 810
BstACI GRCGYC 2 cut(s) 261, 802
BstAPI GCANNNNNTGC 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 767
BstDEI CTNAG 7 cut(s) 191, 339, 347, 505, 687, 947, 1013
BstEII GGTNACC 1 cut(s) 654
BstF5I GGATG 3 cut(s) 48, 490, 675
BstFNI CGCG 2 cut(s) 238, 271
BstHHI GCGC 2 cut(s) 240, 286
BstKTI GATC 7 cut(s) 69, 109, 123, 454, 639, 966, 988
BstMAI GTCTC 3 cut(s) 376, 402, 485
BstMBI GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
BstMWI GCNNNNNNNGC 5 cut(s) 27, 140, 244, 335, 896
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 1 cut(s) 251
BstPI GGTNACC 1 cut(s) 654
BstSCI CCNGG 4 cut(s) 82, 857, 932, 933
BstUI CGCG 2 cut(s) 238, 271
BstV1I GCAGC 2 cut(s) 112, 791
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 5 cut(s) 66, 106, 636, 963, 985
BstYI RGATCY 5 cut(s) 66, 106, 636, 963, 985
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 3 cut(s) 48, 490, 675
BtsIMutI CAGTG 4 cut(s) 330, 747, 806, 892
Cac8I GCNNGC 1 cut(s) 767
CaiI CAGNNNCTG 1 cut(s) 190
CfoI GCGC 2 cut(s) 240, 286
Cfr13I GGNCC 1 cut(s) 88
Cfr9I CCCGGG 1 cut(s) 933
CseI GACGC 3 cut(s) 10, 269, 810
Csp6I GTAC 3 cut(s) 94, 754, 819
CviAII CATG 2 cut(s) 248, 750
CviQI GTAC 3 cut(s) 94, 754, 819
DdeI CTNAG 7 cut(s) 191, 339, 347, 505, 687, 947, 1013
DpnI GATC 7 cut(s) 68, 108, 122, 453, 638, 965, 987
DpnII GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
DraIII CACNNNGTG 1 cut(s) 797
DrdI GACNNNNNNGTC 1 cut(s) 652
DseDI GACNNNNNNGTC 1 cut(s) 652
EciI GGCGGA 2 cut(s) 45, 179
Eco47I GGWCC 1 cut(s) 88
Eco88I CYCGRG 3 cut(s) 221, 562, 933
Eco91I GGTNACC 1 cut(s) 654
EcoO109I RGGNCCY 1 cut(s) 88
EcoO65I GGTNACC 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 736
EcoRII CCWGG 1 cut(s) 857
FaeI CATG 2 cut(s) 251, 753
FalI AAGNNNNNCTT 4 cut(s) 830, 862, 996, 1028
FaqI GGGAC 2 cut(s) 101, 985
FatI CATG 2 cut(s) 247, 749
FauNDI CATATG 2 cut(s) 724, 874
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 6 cut(s) 28, 101, 141, 780, 865, 1018
FokI GGATG 3 cut(s) 55, 497, 662
Fsp4HI GCNGC 6 cut(s) 28, 101, 141, 780, 865, 1018
FspBI CTAG 4 cut(s) 144, 287, 660, 1030
GlaI GCGC 2 cut(s) 239, 285
GluI GCNGC 6 cut(s) 28, 101, 141, 780, 865, 1018
GsaI CCCAGC 2 cut(s) 182, 576
GsuI CTGGAG 3 cut(s) 179, 227, 405
HapII CCGG 3 cut(s) 59, 83, 934
HgaI GACGC 3 cut(s) 10, 269, 810
HhaI GCGC 2 cut(s) 240, 286
Hin1I GRCGYC 2 cut(s) 261, 802
Hin1II CATG 2 cut(s) 251, 753
Hin6I GCGC 2 cut(s) 238, 284
HinP1I GCGC 2 cut(s) 238, 284
HincII GTYRAC 2 cut(s) 253, 522
HindII GTYRAC 2 cut(s) 253, 522
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 5 cut(s) 79, 154, 266, 320, 592
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 3 cut(s) 59, 83, 934
HphI GGTGA 2 cut(s) 88, 648
Hpy166II GTNNAC 3 cut(s) 253, 522, 800
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 3 cut(s) 158, 172, 476
Hpy8I GTNNAC 3 cut(s) 253, 522, 800
Hpy99I CGWCG 3 cut(s) 26, 130, 263
HpyCH4III ACNGT 2 cut(s) 433, 810
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 394, 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 140, 244, 335, 896
HpyF3I CTNAG 7 cut(s) 191, 339, 347, 505, 687, 947, 1013
HpySE526I ACGT 1 cut(s) 125
Hsp92I GRCGYC 2 cut(s) 261, 802
Hsp92II CATG 2 cut(s) 251, 753
HspAI GCGC 2 cut(s) 238, 284
KflI GGGWCCC 1 cut(s) 88
Ksp22I TGATCA 1 cut(s) 451
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
LmnI GCTCC 2 cut(s) 861, 910
Lsp1109I GCAGC 2 cut(s) 112, 791
LweI GCATC 2 cut(s) 381, 767
MaeI CTAG 4 cut(s) 144, 287, 660, 1030
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 2 cut(s) 353, 654
MalI GATC 7 cut(s) 68, 108, 122, 453, 638, 965, 987
MboI GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
MboII GAAGA 2 cut(s) 130, 738
MfeI CAATTG 1 cut(s) 53
MflI RGATCY 5 cut(s) 66, 106, 636, 963, 985
MluCI AATT 5 cut(s) 53, 279, 412, 736, 828
MlyI GAGTC 2 cut(s) 260, 586
MmeI TCCRAC 2 cut(s) 348, 644
MseI TTAA 2 cut(s) 252, 378
MslI CAYNNNNRTG 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 3 cut(s) 59, 83, 934
MspR9I CCNGG 4 cut(s) 84, 859, 934, 935
MunI CAATTG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 2 cut(s) 238, 271
MwoI GCNNNNNNNGC 5 cut(s) 27, 140, 244, 335, 896
NciI CCSGG 3 cut(s) 84, 934, 935
NdeI CATATG 2 cut(s) 724, 874
NdeII GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
NlaIII CATG 2 cut(s) 251, 753
NlaIV GGNNCC 6 cut(s) 89, 90, 445, 462, 703, 863
NmuCI GTSAC 2 cut(s) 353, 654
NspI RCATGY 1 cut(s) 251
NspV TTCGAA 1 cut(s) 152
PaeR7I CTCGAG 2 cut(s) 221, 562
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 3 cut(s) 79, 154, 320
Pfl23II CGTACG 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 6 cut(s) 29, 102, 142, 781, 866, 1019
PleI GAGTC 2 cut(s) 260, 586
PpsI GAGTC 2 cut(s) 260, 586
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 857
PspEI GGTNACC 1 cut(s) 654
PspFI CCCAGC 2 cut(s) 178, 572
PspGI CCWGG 1 cut(s) 857
PspLI CGTACG 1 cut(s) 93
PspN4I GGNNCC 6 cut(s) 89, 90, 445, 462, 703, 863
PspPI GGNCC 1 cut(s) 88
PspPPI RGGWCCY 1 cut(s) 88
PspXI VCTCGAGB 2 cut(s) 221, 562
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 5 cut(s) 66, 106, 636, 963, 985
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 3 cut(s) 95, 755, 820
RsaNI GTAC 3 cut(s) 94, 754, 819
RseI CAYNNNNRTG 1 cut(s) 128
SaqAI TTAA 2 cut(s) 252, 378
SatI GCNGC 6 cut(s) 28, 101, 141, 780, 865, 1018
Sau3AI GATC 7 cut(s) 66, 106, 120, 451, 636, 963, 985
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 2 cut(s) 260, 586
ScrFI CCNGG 4 cut(s) 84, 859, 934, 935
SfaNI GCATC 2 cut(s) 381, 767
Sfr274I CTCGAG 2 cut(s) 221, 562
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 1 cut(s) 88
SlaI CTCGAG 2 cut(s) 221, 562
SmaI CCCGGG 1 cut(s) 935
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 2 cut(s) 221, 562
SmoI CTYRAG 2 cut(s) 221, 562
Sse9I AATT 5 cut(s) 53, 279, 412, 736, 828
SsiI CCGC 9 cut(s) 15, 27, 30, 74, 140, 164, 271, 865, 1018
SspI AATATT 1 cut(s) 515
SspMI CTAG 4 cut(s) 144, 287, 660, 1030
StyD4I CCNGG 4 cut(s) 82, 857, 932, 933
TaaI ACNGT 2 cut(s) 433, 810
TaiI ACGT 1 cut(s) 128
TaqI TCGA 6 cut(s) 128, 152, 222, 563, 644, 826
TasI AATT 5 cut(s) 53, 279, 412, 736, 828
TauI GCSGC 4 cut(s) 30, 143, 867, 1020
TfiI GAWTC 3 cut(s) 79, 154, 320
Tru1I TTAA 2 cut(s) 252, 378
Tru9I TTAA 2 cut(s) 252, 378
TscAI CASTG 4 cut(s) 330, 747, 813, 892
TseFI GTSAC 2 cut(s) 353, 654
TseI GCWGC 2 cut(s) 100, 779
Tsp45I GTSAC 2 cut(s) 353, 654
TspDTI ATGAA 4 cut(s) 405, 570, 576, 738
TspGWI ACGGA 1 cut(s) 748
TspMI CCCGGG 1 cut(s) 933
TspRI CASTG 4 cut(s) 330, 747, 813, 892
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 736
XceI RCATGY 1 cut(s) 251
XhoI CTCGAG 2 cut(s) 221, 562
XmaI CCCGGG 1 cut(s) 933
XspI CTAG 4 cut(s) 144, 287, 660, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.