FvH4_3g15540

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012031
Physical Location & Seq
Forward (+)
9718139 .. 9722470
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15540.t1

Sequence Viewer

Length: 1023 bp
ATGTCGTCGGAGGGCAGCAGAAGAGTGGGTGGAGGAGGGCCGGACTTCGATTTGCCGGATGAGATACTGGCGGTGATTCCGAGCGACCCGTACGAGCAGCTGGATCTGGCGAGGAAGATCACGTCGATGGCGATAGCGGCGAGGGTTTCGAATCTGGAGGCGGAGGTGGGGAGGCTGAGGATGAAGCTGGGCGAGAAAGATAGGGTTATATTTGAGCTTGAGGAGACGGCGTCGCGTCTGCAACGTGTTAATCATGATGCTGACTCGAGGTTGGGGATTGCTCTGGAGGAGAATATGAGGATTTCCAAAGAACGAGATTCGCTGGCAACGACTGCTAAGAAGCTCAGCCGTGACTTGGCCAAATTGGAAACATTTAAGAGGCAACTGATGCAATCTCTAAATGATGATAATTCATCTCCAGCCAGGACTGTTGATATCGGCACCTGTGATCAAGCAGTTCCAAAGGCATATCCAGATGAGGAGACAAATGGCTACTCAGCACAATATTCGTCAACTGGTTCTACTAATATGGGGAACACAACTGATGAAGCCTTGAGGAATGGTGAGCAAAATTTTTCATTGACTCCCTATATCACACCTCGACTTACTCCAACTGCTACTCCAAAGGTCTTTTCGACAACTGGGTCACCTAGAGCATATTCTACCATCCCATCTCCGCAGCAGACCTCTGGTGCCACATCTCCCACAAAATCATATGATGGACGGAATTCAGTGTCTTCTTGGTACCCATCAAGCCAGCAGTCATCAGCAGCTAACTCTCCACCTCATGGACGACCATTGTCAGGGCGGACTCCTCGAATTGATGGAAAGGAGTTCTTTCGTCAGGCCAGGAGCCGCCTATCATATGAGCAGTTCAGTGCATTCCTGGCTAACATCAAGGAGCTAAATGCTAAAAAGCAAACCCGGGAGGAAACTCTGAGGAAAGCAGAGGAGATCTTTGGGACGGATAACAAAGATCTGTATTTATCATTTCAAGGATTGCTAAACCGCAACATACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

37.61

Weight (kDa)

7.76

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 272 - 338 2.8e-35 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 643
Acc65I GGTACC 1 cut(s) 744
AccB1I GGYRCC 3 cut(s) 440, 692, 744
AccB7I CCANNNNNTGG 1 cut(s) 788
AccII CGCG 1 cut(s) 235
AciI CCGC 7 cut(s) 71, 137, 161, 677, 808, 856, 1009
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 1 cut(s) 357
AcsI RAATTY 2 cut(s) 571, 727
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 2 cut(s) 92, 746
AfiI CCNNNNNNNGG 3 cut(s) 355, 788, 803
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 1 cut(s) 995
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 3 cut(s) 422, 848, 885
AjuI GAANNNNNNNTTGG 2 cut(s) 616, 648
AluBI AGCT 6 cut(s) 100, 187, 217, 343, 773, 904
AluI AGCT 6 cut(s) 100, 187, 217, 343, 773, 904
Alw26I GTCTC 2 cut(s) 218, 476
AlwI GGATC 1 cut(s) 111
Ama87I CYCGRG 2 cut(s) 265, 924
AoxI GGCC 3 cut(s) 38, 357, 846
ApeKI GCWGC 4 cut(s) 15, 97, 679, 770
ApoI RAATTY 2 cut(s) 571, 727
Asp718I GGTACC 1 cut(s) 744
AspS9I GGNCC 1 cut(s) 38
AsuC2I CCSGG 2 cut(s) 925, 926
AsuHPI GGTGA 3 cut(s) 85, 575, 639
AsuII TTCGAA 1 cut(s) 149
AvaI CYCGRG 2 cut(s) 265, 924
BalI TGGCCA 1 cut(s) 359
BanI GGYRCC 3 cut(s) 440, 692, 744
BbsI GAAGAC 1 cut(s) 729
BbvCI CCTCAGC 1 cut(s) 176
BbvI GCAGC 4 cut(s) 27, 109, 691, 782
BccI CCATC 6 cut(s) 121, 674, 679, 713, 757, 818
BceAI ACGGC 2 cut(s) 243, 333
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciT130I CCWGG 3 cut(s) 424, 850, 887
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 2 cut(s) 925, 926
BcoDI GTCTC 2 cut(s) 218, 476
BfaI CTAG 1 cut(s) 651
BglII AGATCT 2 cut(s) 954, 976
BisI GCNGC 6 cut(s) 16, 98, 138, 680, 771, 856
BlpI GCTNAGC 1 cut(s) 344
BlsI GCNGC 6 cut(s) 17, 99, 139, 681, 772, 857
Bme1390I CCNGG 5 cut(s) 424, 850, 887, 925, 926
BmeT110I CYCGRG 2 cut(s) 265, 924
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 4 cut(s) 442, 694, 746, 854
BmrFI CCNGG 5 cut(s) 424, 850, 887, 925, 926
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 2 cut(s) 247, 378
BmuI ACTGGG 1 cut(s) 651
BoxI GACNNNNGTC 1 cut(s) 799
BpiI GAAGAC 1 cut(s) 729
BpmI CTGGAG 3 cut(s) 176, 305, 402
Bpu10I CCTNAGC 1 cut(s) 176
Bpu1102I GCTNAGC 1 cut(s) 344
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 239, 574
BpuMI CCSGG 2 cut(s) 925, 926
BsaHI GRCGYC 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 924
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 604, 634
Bsc4I CCNNNNNNNGG 3 cut(s) 355, 788, 803
Bse1I ACTGG 3 cut(s) 72, 520, 646
BseBI CCWGG 3 cut(s) 424, 850, 887
BseDI CCNNGG 1 cut(s) 924
BseGI GGATG 3 cut(s) 64, 186, 666
BseLI CCNNNNNNNGG 3 cut(s) 355, 788, 803
BseMII CTCAG 4 cut(s) 167, 358, 510, 929
BseNI ACTGG 3 cut(s) 72, 520, 646
BseRI GAGGAG 6 cut(s) 48, 236, 302, 494, 804, 965
BseXI GCAGC 4 cut(s) 27, 109, 691, 782
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 3 cut(s) 40, 359, 848
BshNI GGYRCC 3 cut(s) 440, 692, 744
BsiHKCI CYCGRG 2 cut(s) 265, 924
BsiSI CCGG 3 cut(s) 41, 56, 925
BsiWI CGTACG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 976
BslI CCNNNNNNNGG 3 cut(s) 355, 788, 803
BsmAI GTCTC 2 cut(s) 218, 476
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 976
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 3 cut(s) 40, 359, 848
BsoBI CYCGRG 2 cut(s) 265, 924
Bsp119I TTCGAA 1 cut(s) 149
Bsp143I GATC 5 cut(s) 103, 117, 448, 954, 976
Bsp1720I GCTNAGC 1 cut(s) 344
BspACI CCGC 7 cut(s) 71, 137, 161, 677, 808, 856, 1009
BspANI GGCC 3 cut(s) 40, 359, 848
BspCNI CTCAG 4 cut(s) 168, 357, 509, 930
BspFNI CGCG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 4 cut(s) 442, 694, 746, 854
BspPI GGATC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 149
BspT107I GGYRCC 3 cut(s) 440, 692, 744
BsrI ACTGG 3 cut(s) 72, 520, 646
BssECI CCNNGG 1 cut(s) 924
BssMI GATC 5 cut(s) 103, 117, 448, 954, 976
BssNI GRCGYC 1 cut(s) 230
Bst2UI CCWGG 3 cut(s) 424, 850, 887
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 2 cut(s) 332, 388
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 324, 758
BstDEI CTNAG 5 cut(s) 176, 336, 344, 496, 938
BstEII GGTNACC 1 cut(s) 645
BstF5I GGATG 3 cut(s) 64, 186, 666
BstFNI CGCG 1 cut(s) 235
BstKTI GATC 5 cut(s) 106, 120, 451, 957, 979
BstMAI GTCTC 2 cut(s) 218, 476
BstMBI GATC 5 cut(s) 103, 117, 448, 954, 976
BstMWI GCNNNNNNNGC 4 cut(s) 137, 332, 388, 887
BstNI CCWGG 3 cut(s) 424, 850, 887
BstPAI GACNNNNGTC 1 cut(s) 799
BstPI GGTNACC 1 cut(s) 645
BstSCI CCNGG 5 cut(s) 422, 848, 885, 923, 924
BstUI CGCG 1 cut(s) 235
BstV1I GCAGC 4 cut(s) 27, 109, 691, 782
BstV2I GAAGAC 1 cut(s) 729
BstX2I RGATCY 3 cut(s) 103, 954, 976
BstYI RGATCY 3 cut(s) 103, 954, 976
BsuRI GGCC 3 cut(s) 40, 359, 848
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 3 cut(s) 64, 186, 666
BtsIMutI CAGTG 2 cut(s) 738, 883
Cac8I GCNNGC 2 cut(s) 324, 758
CciI TCATGA 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 38
Cfr9I CCCGGG 1 cut(s) 924
CseI GACGC 2 cut(s) 219, 224
Csp6I GTAC 2 cut(s) 91, 745
CviAII CATG 2 cut(s) 254, 788
CviQI GTAC 2 cut(s) 91, 745
DdeI CTNAG 5 cut(s) 176, 336, 344, 496, 938
DpnI GATC 5 cut(s) 105, 119, 450, 956, 978
DpnII GATC 5 cut(s) 103, 117, 448, 954, 976
DrdI GACNNNNNNGTC 1 cut(s) 643
DseDI GACNNNNNNGTC 1 cut(s) 643
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 2 cut(s) 176, 823
Eco32I GATATC 1 cut(s) 436
Eco88I CYCGRG 2 cut(s) 265, 924
Eco91I GGTNACC 1 cut(s) 645
EcoO65I GGTNACC 1 cut(s) 645
EcoRI GAATTC 1 cut(s) 727
EcoRII CCWGG 3 cut(s) 422, 848, 885
EcoRV GATATC 1 cut(s) 436
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 2 cut(s) 257, 791
FalI AAGNNNNNCTT 2 cut(s) 821, 853
FaqI GGGAC 1 cut(s) 976
FatI CATG 2 cut(s) 253, 787
FauNDI CATATG 2 cut(s) 715, 865
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 6 cut(s) 16, 98, 138, 680, 771, 856
FokI GGATG 3 cut(s) 71, 193, 653
Fsp4HI GCNGC 6 cut(s) 16, 98, 138, 680, 771, 856
FspBI CTAG 1 cut(s) 651
GluI GCNGC 6 cut(s) 16, 98, 138, 680, 771, 856
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 3 cut(s) 176, 305, 402
HaeIII GGCC 3 cut(s) 40, 359, 848
HapII CCGG 3 cut(s) 41, 56, 925
HgaI GACGC 2 cut(s) 219, 224
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 2 cut(s) 257, 791
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HinfI GANTC 6 cut(s) 76, 151, 263, 317, 583, 811
HpaII CCGG 3 cut(s) 41, 56, 925
HphI GGTGA 3 cut(s) 85, 575, 639
Hpy166II GTNNAC 1 cut(s) 513
Hpy188I TCNGA 3 cut(s) 10, 81, 939
Hpy188III TCNNGA 4 cut(s) 155, 254, 284, 473
Hpy8I GTNNAC 1 cut(s) 513
Hpy99I CGWCG 3 cut(s) 10, 127, 235
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 2 cut(s) 122, 244
HpyCH4V TGCA 3 cut(s) 241, 391, 881
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 332, 388, 887
HpyF3I CTNAG 5 cut(s) 176, 336, 344, 496, 938
HpySE526I ACGT 2 cut(s) 122, 244
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 2 cut(s) 257, 791
KpnI GGTACC 1 cut(s) 748
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 5 cut(s) 103, 117, 448, 954, 976
LmnI GCTCC 2 cut(s) 852, 901
Lsp1109I GCAGC 4 cut(s) 27, 109, 691, 782
LweI GCATC 2 cut(s) 247, 378
MaeI CTAG 1 cut(s) 651
MaeII ACGT 2 cut(s) 122, 244
MaeIII GTNAC 2 cut(s) 350, 645
MalI GATC 5 cut(s) 105, 119, 450, 956, 978
MboI GATC 5 cut(s) 103, 117, 448, 954, 976
MboII GAAGA 3 cut(s) 33, 127, 729
MflI RGATCY 3 cut(s) 103, 954, 976
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 5 cut(s) 362, 409, 571, 727, 819
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 3 cut(s) 257, 577, 805
MmeI TCCRAC 1 cut(s) 635
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 2 cut(s) 249, 375
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 3 cut(s) 41, 56, 925
MspR9I CCNGG 5 cut(s) 424, 850, 887, 925, 926
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 3 cut(s) 424, 850, 887
MvnI CGCG 1 cut(s) 235
MwoI GCNNNNNNNGC 4 cut(s) 137, 332, 388, 887
NciI CCSGG 2 cut(s) 925, 926
NdeI CATATG 2 cut(s) 715, 865
NdeII GATC 5 cut(s) 103, 117, 448, 954, 976
NlaIII CATG 2 cut(s) 257, 791
NlaIV GGNNCC 4 cut(s) 442, 694, 746, 854
NmuCI GTSAC 2 cut(s) 350, 645
NspV TTCGAA 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 265
PagI TCATGA 1 cut(s) 253
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 326
PctI GAATGC 1 cut(s) 881
PfeI GAWTC 3 cut(s) 76, 151, 317
Pfl23II CGTACG 1 cut(s) 90
PflFI GACNNNGTC 1 cut(s) 229
PflMI CCANNNNNTGG 1 cut(s) 788
PkrI GCNGC 6 cut(s) 17, 99, 139, 681, 772, 857
PleI GAGTC 3 cut(s) 257, 577, 805
PpsI GAGTC 3 cut(s) 257, 577, 805
PshAI GACNNNNGTC 1 cut(s) 799
Psp6I CCWGG 3 cut(s) 422, 848, 885
PspEI GGTNACC 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 3 cut(s) 422, 848, 885
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 4 cut(s) 442, 694, 746, 854
PspPI GGNCC 1 cut(s) 38
PspXI VCTCGAGB 1 cut(s) 265
PsuI RGATCY 3 cut(s) 103, 954, 976
PsyI GACNNNGTC 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 2 cut(s) 92, 746
RsaNI GTAC 2 cut(s) 91, 745
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 2 cut(s) 249, 375
SatI GCNGC 6 cut(s) 16, 98, 138, 680, 771, 856
Sau3AI GATC 5 cut(s) 103, 117, 448, 954, 976
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 3 cut(s) 257, 577, 805
ScrFI CCNGG 5 cut(s) 424, 850, 887, 925, 926
SfaNI GCATC 2 cut(s) 247, 378
Sfr274I CTCGAG 1 cut(s) 265
SfuI TTCGAA 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 265
SmaI CCCGGG 1 cut(s) 926
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 3 cut(s) 218, 265, 553
SmoI CTYRAG 3 cut(s) 218, 265, 553
Sse9I AATT 5 cut(s) 362, 409, 571, 727, 819
SsiI CCGC 7 cut(s) 71, 137, 161, 677, 808, 856, 1009
SspI AATATT 1 cut(s) 506
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 5 cut(s) 422, 848, 885, 923, 924
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 2 cut(s) 125, 247
TaqI TCGA 7 cut(s) 48, 125, 149, 266, 601, 635, 817
TasI AATT 5 cut(s) 362, 409, 571, 727, 819
TauI GCSGC 2 cut(s) 140, 858
TfiI GAWTC 3 cut(s) 76, 151, 317
Tru1I TTAA 2 cut(s) 249, 375
Tru9I TTAA 2 cut(s) 249, 375
TscAI CASTG 2 cut(s) 738, 883
TseFI GTSAC 2 cut(s) 350, 645
TseI GCWGC 4 cut(s) 15, 97, 679, 770
Tsp45I GTSAC 2 cut(s) 350, 645
TspDTI ATGAA 4 cut(s) 197, 402, 561, 567
TspGWI ACGGA 2 cut(s) 739, 980
TspMI CCCGGG 1 cut(s) 924
TspRI CASTG 2 cut(s) 738, 883
Tth111I GACNNNGTC 1 cut(s) 229
Van91I CCANNNNNTGG 1 cut(s) 788
XapI RAATTY 2 cut(s) 571, 727
XhoI CTCGAG 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 924
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.