MD05G1222300.v1.1

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012031
Physical Location & Seq
Reverse (-)
35485858 .. 35489844
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1222300.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGTCGCAGGGCAGTGGAAGCGTCGGCGGCGGTGGAGGTGGCGGTGGGCTCGACTTTCAGCTGCCGGACGAGATCTTATCGGTGATACCGAGCGACCCGTACGACCAGCTGGATCTGGCGCGGAAGATCACGTCGATGGCCATAGCGTCTAGGGTTTCGAAGCTGGAGTCTGAGATGGGGAAGATGAGGCAGAAGCTGCAGGACAAAGATAGGGTTGTGTTTGAGCTCGAGGAAAGGGTCTCGCGCCTCCAGCACGCCAACCACGAGGCTGAGTCCCGCCTGAGAATTTCCTTGGATGAGAATTTGAAGCTTTCCAAAGAGCGAGATTCGTTGGCATTGACTACTAAAAAGCTCAGCCGTGACTTATCAAAGTTGGAGACATTTAAGAGACAACTAATGCAGTCACTAAATGATGATAATGGATCTCCAGCTGAAACTGTTGATATTGGCACTGTTCCGAAGGCATATCCTGATAAGGATGAGGAGACAAATGGCTACATAGGACAATATTCCTCAAGTGGTTCCACAGATAAGGGAGCAACTGATGAAGTCTTGAAGAATTCCACTCAAGGATTTTCTATAACTCCATATATCACTCCGCGGCTTACTCCAACTGGAACTCCAAAGATTATTTCCACAAGTGGGTCACCTAGAGGGTATTCTGCCATTTCTTCTCCTCAGCAATCATCTGGTGCCACATCTCCCCAAAAACCCGCCTATAAAGAACGGACTTCATTCTCTTCATGGTACTCATCAAGCCAGCAGTCATCAGCAGCAAACTCTCCACCTCGTGGGCGTTCACATTCAGGGCGCACTCCTCGAATTGATGGAAAAGAGTTCTTTCGTCAAGCCAGGAGTCGTTTATCATATGAGCAGTTCAGTGCATTTCTGGCTAACATCAAGGAACTAAATGCTCATAAACAAACCAGAGAGGAAACTTTAAGGAAAGCAGAAGAAATATTCGGGACTGACAACAAAGACCTATATTTATCATTCCAAGGGTTGCTTAGCCGCAATATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

37.58

Weight (kDa)

9.0

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 273 - 339 8.3e-36 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 692
AccB7I CCANNNNNTGG 1 cut(s) 791
AccII CGCG 3 cut(s) 121, 244, 601
AciI CCGC 9 cut(s) 27, 30, 42, 121, 277, 599, 601, 714, 1012
AclWI GGATC 2 cut(s) 120, 430
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 3 cut(s) 285, 301, 559
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 2 cut(s) 101, 749
AfiI CCNNNNNNNGG 2 cut(s) 642, 791
AgsI TTSAA 2 cut(s) 307, 556
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 1 cut(s) 851
AjuI GAANNNNNNNTTGG 2 cut(s) 616, 648
AluBI AGCT 8 cut(s) 61, 109, 163, 196, 226, 310, 352, 431
AluI AGCT 8 cut(s) 61, 109, 163, 196, 226, 310, 352, 431
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 4 cut(s) 244, 371, 382, 479
AlwI GGATC 2 cut(s) 120, 430
AlwNI CAGNNNCTG 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 3 cut(s) 61, 196, 773
ApoI RAATTY 3 cut(s) 285, 301, 559
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 3 cut(s) 121, 246, 813
AsuHPI GGTGA 2 cut(s) 94, 639
AsuII TTCGAA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 227
BalI TGGCCA 1 cut(s) 140
BanI GGYRCC 1 cut(s) 692
BanII GRGCYC 2 cut(s) 51, 228
BauI CACGAG 2 cut(s) 263, 789
Bbv12I GWGCWC 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 3 cut(s) 48, 183, 785
BccI CCATC 3 cut(s) 130, 169, 821
BceAI ACGGC 1 cut(s) 342
BciT130I CCWGG 1 cut(s) 853
BcoDI GTCTC 4 cut(s) 244, 371, 382, 479
BfaI CTAG 3 cut(s) 150, 651, 1024
BfmI CTRYAG 1 cut(s) 197
BglII AGATCT 1 cut(s) 72
BisI GCNGC 6 cut(s) 28, 62, 197, 602, 774, 1012
BlpI GCTNAGC 2 cut(s) 353, 1007
BlsI GCNGC 6 cut(s) 29, 63, 198, 603, 775, 1013
Bme1390I CCNGG 1 cut(s) 853
BmeT110I CYCGRG 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 132
BmiI GGNNCC 2 cut(s) 523, 694
BmrFI CCNGG 1 cut(s) 853
BpmI CTGGAG 3 cut(s) 185, 233, 411
Bpu10I CCTNAGC 1 cut(s) 678
Bpu1102I GCTNAGC 2 cut(s) 353, 1007
Bpu14I TTCGAA 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 499, 552
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 3 cut(s) 291, 599, 997
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bsc4I CCNNNNNNNGG 2 cut(s) 642, 791
Bse1I ACTGG 1 cut(s) 619
BseBI CCWGG 1 cut(s) 853
BseDI CCNNGG 3 cut(s) 291, 599, 997
BseGI GGATG 2 cut(s) 301, 484
BseLI CCNNNNNNNGG 2 cut(s) 642, 791
BseMII CTCAG 5 cut(s) 162, 261, 272, 367, 692
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 3 cut(s) 497, 666, 807
BseXI GCAGC 3 cut(s) 48, 183, 785
Bsh1236I CGCG 3 cut(s) 121, 244, 601
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 1 cut(s) 65
BsiWI CGTACG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 259, 979
BslI CCNNNNNNNGG 2 cut(s) 642, 791
BsmAI GTCTC 4 cut(s) 244, 371, 382, 479
BsmFI GGGAC 2 cut(s) 259, 979
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 51, 228
Bsp143I GATC 4 cut(s) 72, 112, 126, 422
Bsp1720I GCTNAGC 2 cut(s) 353, 1007
BspACI CCGC 9 cut(s) 27, 30, 42, 121, 277, 599, 601, 714, 1012
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 5 cut(s) 163, 262, 273, 366, 691
BspFNI CGCG 3 cut(s) 121, 244, 601
BspLI GGNNCC 2 cut(s) 523, 694
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 2 cut(s) 120, 430
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 244
BsrI ACTGG 1 cut(s) 619
BssECI CCNNGG 3 cut(s) 291, 599, 997
BssMI GATC 4 cut(s) 72, 112, 126, 422
BssSI CACGAG 2 cut(s) 263, 789
BssT1I CCWWGG 2 cut(s) 291, 997
Bst2BI CACGAG 2 cut(s) 263, 789
Bst2UI CCWGG 1 cut(s) 853
Bst4CI ACNGT 2 cut(s) 439, 454
Bst6I CTCTTC 1 cut(s) 745
BstAPI GCANNNNNTGC 1 cut(s) 196
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 255, 761
BstDEI CTNAG 6 cut(s) 171, 270, 281, 353, 678, 1007
BstDSI CCRYGG 1 cut(s) 599
BstEII GGTNACC 1 cut(s) 645
BstF5I GGATG 2 cut(s) 301, 484
BstFNI CGCG 3 cut(s) 121, 244, 601
BstHHI GCGC 3 cut(s) 121, 246, 813
BstKTI GATC 4 cut(s) 75, 115, 129, 425
BstMAI GTCTC 4 cut(s) 244, 371, 382, 479
BstMBI GATC 4 cut(s) 72, 112, 126, 422
BstMWI GCNNNNNNNGC 5 cut(s) 18, 27, 196, 250, 890
BstNI CCWGG 1 cut(s) 853
BstPI GGTNACC 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 851
BstSFI CTRYAG 1 cut(s) 197
BstUI CGCG 3 cut(s) 121, 244, 601
BstV1I GCAGC 3 cut(s) 48, 183, 785
BstX2I RGATCY 3 cut(s) 72, 112, 422
BstYI RGATCY 3 cut(s) 72, 112, 422
BsuRI GGCC 1 cut(s) 140
BtgI CCRYGG 1 cut(s) 599
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 2 cut(s) 301, 484
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 3 cut(s) 19, 450, 886
Cac8I GCNNGC 2 cut(s) 255, 761
CaiI CAGNNNCTG 1 cut(s) 196
CfoI GCGC 3 cut(s) 121, 246, 813
Cfr42I CCGCGG 1 cut(s) 602
CseI GACGC 2 cut(s) 10, 135
Csp6I GTAC 2 cut(s) 100, 748
CviAII CATG 1 cut(s) 744
CviQI GTAC 2 cut(s) 100, 748
DdeI CTNAG 6 cut(s) 171, 270, 281, 353, 678, 1007
DpnI GATC 4 cut(s) 74, 114, 128, 424
DpnII GATC 4 cut(s) 72, 112, 126, 422
DraIII CACNNNGTG 1 cut(s) 791
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 745
EarI CTCTTC 1 cut(s) 745
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 291, 997
Eco24I GRGCYC 2 cut(s) 51, 228
Eco31I GGTCTC 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 227
Eco91I GGTNACC 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 645
EcoRI GAATTC 1 cut(s) 559
EcoRII CCWGG 1 cut(s) 851
EcoT14I CCWWGG 2 cut(s) 291, 997
EcoT38I GRGCYC 2 cut(s) 51, 228
ErhI CCWWGG 2 cut(s) 291, 997
FaeI CATG 1 cut(s) 747
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FaqI GGGAC 2 cut(s) 259, 979
FatI CATG 1 cut(s) 743
FauI CCCGC 2 cut(s) 284, 721
FauNDI CATATG 1 cut(s) 868
Fnu4HI GCNGC 6 cut(s) 28, 62, 197, 602, 774, 1012
FokI GGATG 2 cut(s) 308, 491
FriOI GRGCYC 2 cut(s) 51, 228
Fsp4HI GCNGC 6 cut(s) 28, 62, 197, 602, 774, 1012
FspBI CTAG 3 cut(s) 150, 651, 1024
GlaI GCGC 3 cut(s) 120, 245, 812
GluI GCNGC 6 cut(s) 28, 62, 197, 602, 774, 1012
GsuI CTGGAG 3 cut(s) 185, 233, 411
HaeIII GGCC 1 cut(s) 140
HapII CCGG 1 cut(s) 65
HgaI GACGC 2 cut(s) 10, 135
HhaI GCGC 3 cut(s) 121, 246, 813
Hin1II CATG 1 cut(s) 747
Hin6I GCGC 3 cut(s) 119, 244, 811
HinP1I GCGC 3 cut(s) 119, 244, 811
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 4 cut(s) 167, 272, 326, 856
HpaII CCGG 1 cut(s) 65
HphI GGTGA 2 cut(s) 94, 639
Hpy166II GTNNAC 1 cut(s) 800
Hpy188I TCNGA 2 cut(s) 172, 459
Hpy188III TCNNGA 3 cut(s) 470, 553, 964
Hpy8I GTNNAC 1 cut(s) 800
Hpy99I CGWCG 2 cut(s) 26, 136
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 2 cut(s) 439, 454
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 3 cut(s) 199, 400, 884
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 27, 196, 250, 890
HpyF3I CTNAG 6 cut(s) 171, 270, 281, 353, 678, 1007
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 1 cut(s) 747
HspAI GCGC 3 cut(s) 119, 244, 811
KspI CCGCGG 1 cut(s) 602
Kzo9I GATC 4 cut(s) 72, 112, 126, 422
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 3 cut(s) 48, 183, 785
MaeI CTAG 3 cut(s) 150, 651, 1024
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 359, 402, 645
MalI GATC 4 cut(s) 74, 114, 128, 424
MboI GATC 4 cut(s) 72, 112, 126, 422
MboII GAAGA 6 cut(s) 136, 193, 568, 663, 732, 965
MflI RGATCY 3 cut(s) 72, 112, 422
MhlI GDGCHC 2 cut(s) 51, 228
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 4 cut(s) 285, 301, 559, 822
MluNI TGGCCA 1 cut(s) 140
MlyI GAGTC 3 cut(s) 176, 281, 865
MmeI TCCRAC 2 cut(s) 354, 635
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 2 cut(s) 384, 941
MslI CAYNNNNRTG 1 cut(s) 134
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 4 cut(s) 61, 109, 431, 601
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 853
MvaI CCWGG 1 cut(s) 853
MvnI CGCG 3 cut(s) 121, 244, 601
MwoI GCNNNNNNNGC 5 cut(s) 18, 27, 196, 250, 890
NdeI CATATG 1 cut(s) 868
NdeII GATC 4 cut(s) 72, 112, 126, 422
NlaIII CATG 1 cut(s) 747
NlaIV GGNNCC 2 cut(s) 523, 694
NmuCI GTSAC 3 cut(s) 359, 402, 645
NspV TTCGAA 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 227
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 261
PfeI GAWTC 1 cut(s) 326
Pfl23II CGTACG 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 791
PkrI GCNGC 6 cut(s) 29, 63, 198, 603, 775, 1013
PleI GAGTC 3 cut(s) 175, 280, 864
PpsI GAGTC 3 cut(s) 175, 280, 864
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 851
PspEI GGTNACC 1 cut(s) 645
PspGI CCWGG 1 cut(s) 851
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 523, 694
PspXI VCTCGAGB 1 cut(s) 227
PstI CTGCAG 1 cut(s) 201
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 3 cut(s) 72, 112, 422
PvuII CAGCTG 3 cut(s) 61, 109, 431
RsaI GTAC 2 cut(s) 101, 749
RsaNI GTAC 2 cut(s) 100, 748
RseI CAYNNNNRTG 1 cut(s) 134
SacI GAGCTC 1 cut(s) 228
SacII CCGCGG 1 cut(s) 602
SaqAI TTAA 2 cut(s) 384, 941
SatI GCNGC 6 cut(s) 28, 62, 197, 602, 774, 1012
Sau3AI GATC 4 cut(s) 72, 112, 126, 422
SchI GAGTC 3 cut(s) 176, 281, 865
ScrFI CCNGG 1 cut(s) 853
SduI GDGCHC 2 cut(s) 51, 228
SfcI CTRYAG 1 cut(s) 197
Sfr274I CTCGAG 1 cut(s) 227
Sfr303I CCGCGG 1 cut(s) 602
SfuI TTCGAA 1 cut(s) 158
SgrBI CCGCGG 1 cut(s) 602
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 3 cut(s) 227, 514, 567
SmoI CTYRAG 3 cut(s) 227, 514, 567
Sse9I AATT 4 cut(s) 285, 301, 559, 822
SsiI CCGC 9 cut(s) 27, 30, 42, 121, 277, 599, 601, 714, 1012
SspI AATATT 2 cut(s) 509, 960
SspMI CTAG 3 cut(s) 150, 651, 1024
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 851
StyI CCWWGG 2 cut(s) 291, 997
TaaI ACNGT 2 cut(s) 439, 454
TaiI ACGT 1 cut(s) 134
TaqI TCGA 5 cut(s) 51, 134, 158, 228, 820
TasI AATT 4 cut(s) 285, 301, 559, 822
TauI GCSGC 3 cut(s) 30, 604, 1014
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 384, 941
Tru9I TTAA 2 cut(s) 384, 941
TscAI CASTG 3 cut(s) 19, 457, 886
TseFI GTSAC 3 cut(s) 359, 402, 645
TseI GCWGC 3 cut(s) 61, 196, 773
Tsp45I GTSAC 3 cut(s) 359, 402, 645
TspDTI ATGAA 3 cut(s) 561, 723, 732
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 3 cut(s) 19, 457, 886
Van91I CCANNNNNTGG 1 cut(s) 791
XapI RAATTY 3 cut(s) 285, 301, 559
XhoI CTCGAG 1 cut(s) 227
XspI CTAG 3 cut(s) 150, 651, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.