Rroxscaffold_1G00053140

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012031
Physical Location & Seq
Reverse (-)
73999987 .. 74004272
4286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053140.1

Sequence Viewer

Length: 990 bp
ATGGATTTTCAATTGCCGGACGAGATCTTGGCGGTGATTCCGGGGGACCCGTACGAGCAGCTGGATCTGGCGAGGAAGATCACGTCGATGGCGATAGCGGCTAGGGTTTCGAATCTGGAGGCGGAGGTGGGGAGGCTGAGGCAGAAGCTGAGCGAGAAAGATAGGGTTATTTTTGACCTCGAGGAACGAGCCTCGCGCCTCCAGCATGTTAACCACGACGCCGACTCGCGGTTGAGAATTGCGCTAGAGGAGAATATGAGGCTTTCCAAAGAACGAGATTCAGTGGCAATGACTGCTAAGAAGCTTAGCCGTGACTTGGCAAAGTTGGAAACATTTAAGAGACAACTGATGCAGTCTCTAAATGATGATAATTCATCTCCAGCCAAAACTGTTGATATTGGCACCTGTGATCAATCGGTTCCAAAGGCATATCCAGATAAGGATGAGGAGACAAATGGCTACTCAGCCCAATATTCGTCAACTGGTTCTACTGATATGGGGAACACAACTGATGAAGCCTTGAGGAATGCTGGGCAAAAGTTTTCGTTGACTCCCTATATCACACCTCGGCTTACTCCAACTGGAACACCAAAGATCTTTTCGACAAGTGGGTCACCTACAGCATATTCTACCATCCCATCTCCTCAGCAGACCTCCGGTGCCACATCTCCCACAAAATCATATGATGGACGGAATTCAGTGTCTTCATGGTACCCATCAAGCCAGCAGTCATCAGCAGCAAACTCTCCACCTCGTGGACGCCCACTGTCAGGTCGTACTCCTCGAATTGATGGAAAGGAGTTCTTTCGTCAAGCCAGGAGCCGCCTATCATATGAGCAGTTCAGTGCATTTCTGGCTAACATCAAGGAGCTAAATGCTCAAAAGCAAACCCGGGAGGAAACTCTAAGGAAAGCAGAGGAGATCTTTGGGACAGATAACAAAGATCTATATTTATCATTTCAAGGATTGCTTAGCCGCAACATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

36.69

Weight (kDa)

8.46

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 261 - 327 2.2e-35 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 610
AbsI CCTCGAGG 1 cut(s) 179
Acc65I GGTACC 1 cut(s) 711
AccB1I GGYRCC 3 cut(s) 401, 659, 711
AccB7I CCANNNNNTGG 1 cut(s) 755
AccII CGCG 2 cut(s) 196, 229
AciI CCGC 6 cut(s) 32, 98, 122, 229, 823, 976
AclWI GGATC 1 cut(s) 72
AcsI RAATTY 1 cut(s) 694
AcyI GRCGYC 2 cut(s) 219, 760
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 3 cut(s) 53, 713, 778
AfiI CCNNNNNNNGG 4 cut(s) 228, 316, 755, 770
AgsI TTSAA 2 cut(s) 11, 962
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 1 cut(s) 815
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 4 cut(s) 61, 148, 304, 871
AluI AGCT 4 cut(s) 61, 148, 304, 871
Alw26I GTCTC 3 cut(s) 334, 360, 443
AlwI GGATC 1 cut(s) 72
AlwNI CAGNNNCTG 1 cut(s) 148
Ama87I CYCGRG 2 cut(s) 179, 891
ApeKI GCWGC 2 cut(s) 58, 737
ApoI RAATTY 1 cut(s) 694
Asp718I GGTACC 1 cut(s) 711
AspLEI GCGC 2 cut(s) 198, 244
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 3 cut(s) 42, 892, 893
AsuHPI GGTGA 2 cut(s) 46, 606
AsuII TTCGAA 1 cut(s) 110
AvaI CYCGRG 2 cut(s) 179, 891
AvaII GGWCC 1 cut(s) 46
BanI GGYRCC 3 cut(s) 401, 659, 711
BauI CACGAG 1 cut(s) 753
BbsI GAAGAC 1 cut(s) 696
BbvCI CCTCAGC 2 cut(s) 137, 645
BbvI GCAGC 2 cut(s) 70, 749
BccI CCATC 6 cut(s) 82, 641, 646, 680, 724, 785
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 817
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 3 cut(s) 42, 892, 893
BcoDI GTCTC 3 cut(s) 334, 360, 443
BfaI CTAG 3 cut(s) 102, 245, 988
BfmI CTRYAG 1 cut(s) 618
BglII AGATCT 4 cut(s) 24, 594, 921, 943
BisI GCNGC 5 cut(s) 59, 99, 738, 823, 976
BlpI GCTNAGC 3 cut(s) 149, 305, 971
BlsI GCNGC 5 cut(s) 60, 100, 739, 824, 977
Bme1390I CCNGG 4 cut(s) 42, 817, 892, 893
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 2 cut(s) 179, 891
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 7 cut(s) 47, 48, 403, 420, 661, 713, 821
BmrFI CCNGG 4 cut(s) 42, 817, 892, 893
BmsI GCATC 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 696
BpmI CTGGAG 3 cut(s) 137, 185, 363
Bpu10I CCTNAGC 2 cut(s) 137, 645
Bpu1102I GCTNAGC 3 cut(s) 149, 305, 971
Bpu14I TTCGAA 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 541
BpuMI CCSGG 3 cut(s) 42, 892, 893
BsaHI GRCGYC 2 cut(s) 219, 760
BsaJI CCNNGG 3 cut(s) 41, 566, 891
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 316, 755, 770
Bse1I ACTGG 2 cut(s) 487, 586
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 817
BseDI CCNNGG 3 cut(s) 41, 566, 891
BseGI GGATG 2 cut(s) 448, 633
BseLI CCNNNNNNNGG 4 cut(s) 228, 316, 755, 770
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 4 cut(s) 128, 140, 477, 659
BseNI ACTGG 2 cut(s) 487, 586
BseRI GAGGAG 5 cut(s) 263, 461, 633, 771, 932
BseXI GCAGC 2 cut(s) 70, 749
BseYI CCCAGC 1 cut(s) 530
Bsh1236I CGCG 2 cut(s) 196, 229
BshNI GGYRCC 3 cut(s) 401, 659, 711
BsiHKCI CYCGRG 2 cut(s) 179, 891
BsiSI CCGG 4 cut(s) 17, 41, 657, 892
BsiWI CGTACG 1 cut(s) 51
BslFI GGGAC 2 cut(s) 59, 943
BslI CCNNNNNNNGG 4 cut(s) 228, 316, 755, 770
BsmAI GTCTC 3 cut(s) 334, 360, 443
BsmFI GGGAC 2 cut(s) 59, 943
BsmI GAATGC 1 cut(s) 532
BsoBI CYCGRG 2 cut(s) 179, 891
Bsp119I TTCGAA 1 cut(s) 110
Bsp143I GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
Bsp1720I GCTNAGC 3 cut(s) 149, 305, 971
BspACI CCGC 6 cut(s) 32, 98, 122, 229, 823, 976
BspCNI CTCAG 4 cut(s) 129, 141, 476, 658
BspFNI CGCG 2 cut(s) 196, 229
BspLI GGNNCC 7 cut(s) 47, 48, 403, 420, 661, 713, 821
BspPI GGATC 1 cut(s) 72
BspT104I TTCGAA 1 cut(s) 110
BspT107I GGYRCC 3 cut(s) 401, 659, 711
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 2 cut(s) 487, 586
BssECI CCNNGG 3 cut(s) 41, 566, 891
BssMI GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
BssNI GRCGYC 2 cut(s) 219, 760
BssSI CACGAG 1 cut(s) 753
Bst2BI CACGAG 1 cut(s) 753
Bst2UI CCWGG 1 cut(s) 817
Bst4CI ACNGT 2 cut(s) 391, 768
BstACI GRCGYC 2 cut(s) 219, 760
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 725
BstDEI CTNAG 8 cut(s) 137, 149, 297, 305, 463, 645, 905, 971
BstEII GGTNACC 1 cut(s) 612
BstF5I GGATG 2 cut(s) 448, 633
BstFNI CGCG 2 cut(s) 196, 229
BstHHI GCGC 2 cut(s) 198, 244
BstKTI GATC 7 cut(s) 27, 67, 81, 412, 597, 924, 946
BstMAI GTCTC 3 cut(s) 334, 360, 443
BstMBI GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
BstMWI GCNNNNNNNGC 4 cut(s) 98, 202, 293, 854
BstNI CCWGG 1 cut(s) 817
BstNSI RCATGY 1 cut(s) 209
BstPI GGTNACC 1 cut(s) 612
BstSCI CCNGG 4 cut(s) 40, 815, 890, 891
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 2 cut(s) 196, 229
BstV1I GCAGC 2 cut(s) 70, 749
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 5 cut(s) 24, 64, 594, 921, 943
BstYI RGATCY 5 cut(s) 24, 64, 594, 921, 943
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 448, 633
BtsIMutI CAGTG 4 cut(s) 288, 705, 764, 850
Cac8I GCNNGC 1 cut(s) 725
CaiI CAGNNNCTG 1 cut(s) 148
CfoI GCGC 2 cut(s) 198, 244
Cfr13I GGNCC 1 cut(s) 46
Cfr9I CCCGGG 1 cut(s) 891
CseI GACGC 2 cut(s) 227, 768
Csp6I GTAC 3 cut(s) 52, 712, 777
CviAII CATG 2 cut(s) 206, 708
CviQI GTAC 3 cut(s) 52, 712, 777
DdeI CTNAG 8 cut(s) 137, 149, 297, 305, 463, 645, 905, 971
DpnI GATC 7 cut(s) 26, 66, 80, 411, 596, 923, 945
DpnII GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
DraIII CACNNNGTG 1 cut(s) 755
DrdI GACNNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 610
EciI GGCGGA 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 46
Eco88I CYCGRG 2 cut(s) 179, 891
Eco91I GGTNACC 1 cut(s) 612
EcoO109I RGGNCCY 1 cut(s) 46
EcoO65I GGTNACC 1 cut(s) 612
EcoRI GAATTC 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 815
FaeI CATG 2 cut(s) 209, 711
FalI AAGNNNNNCTT 4 cut(s) 788, 820, 954, 986
FaqI GGGAC 2 cut(s) 59, 943
FatI CATG 2 cut(s) 205, 707
FauNDI CATATG 2 cut(s) 682, 832
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 5 cut(s) 59, 99, 738, 823, 976
FokI GGATG 2 cut(s) 455, 620
Fsp4HI GCNGC 5 cut(s) 59, 99, 738, 823, 976
FspBI CTAG 3 cut(s) 102, 245, 988
GlaI GCGC 2 cut(s) 197, 243
GluI GCNGC 5 cut(s) 59, 99, 738, 823, 976
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 3 cut(s) 137, 185, 363
HapII CCGG 4 cut(s) 17, 41, 657, 892
HgaI GACGC 2 cut(s) 227, 768
HhaI GCGC 2 cut(s) 198, 244
Hin1I GRCGYC 2 cut(s) 219, 760
Hin1II CATG 2 cut(s) 209, 711
Hin6I GCGC 2 cut(s) 196, 242
HinP1I GCGC 2 cut(s) 196, 242
HincII GTYRAC 3 cut(s) 211, 480, 549
HindII GTYRAC 3 cut(s) 211, 480, 549
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 5 cut(s) 37, 112, 224, 278, 550
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 4 cut(s) 17, 41, 657, 892
HphI GGTGA 2 cut(s) 46, 606
Hpy166II GTNNAC 4 cut(s) 211, 480, 549, 758
Hpy188III TCNNGA 2 cut(s) 116, 434
Hpy8I GTNNAC 4 cut(s) 211, 480, 549, 758
Hpy99I CGWCG 2 cut(s) 88, 221
HpyCH4III ACNGT 2 cut(s) 391, 768
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 2 cut(s) 352, 848
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 202, 293, 854
HpyF3I CTNAG 8 cut(s) 137, 149, 297, 305, 463, 645, 905, 971
HpySE526I ACGT 1 cut(s) 83
Hsp92I GRCGYC 2 cut(s) 219, 760
Hsp92II CATG 2 cut(s) 209, 711
HspAI GCGC 2 cut(s) 196, 242
KflI GGGWCCC 1 cut(s) 46
KpnI GGTACC 1 cut(s) 715
Ksp22I TGATCA 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
LmnI GCTCC 2 cut(s) 819, 868
Lsp1109I GCAGC 2 cut(s) 70, 749
LweI GCATC 1 cut(s) 339
MaeI CTAG 3 cut(s) 102, 245, 988
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 311, 612
MalI GATC 7 cut(s) 26, 66, 80, 411, 596, 923, 945
MboI GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
MboII GAAGA 2 cut(s) 88, 696
MfeI CAATTG 1 cut(s) 11
MflI RGATCY 5 cut(s) 24, 64, 594, 921, 943
MluCI AATT 5 cut(s) 11, 237, 370, 694, 786
MlyI GAGTC 2 cut(s) 218, 544
MmeI TCCRAC 2 cut(s) 306, 602
MseI TTAA 2 cut(s) 210, 336
MslI CAYNNNNRTG 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 4 cut(s) 17, 41, 657, 892
MspR9I CCNGG 4 cut(s) 42, 817, 892, 893
MunI CAATTG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 1 cut(s) 817
MvnI CGCG 2 cut(s) 196, 229
MwoI GCNNNNNNNGC 4 cut(s) 98, 202, 293, 854
NciI CCSGG 3 cut(s) 42, 892, 893
NdeI CATATG 2 cut(s) 682, 832
NdeII GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
NlaIII CATG 2 cut(s) 209, 711
NlaIV GGNNCC 7 cut(s) 47, 48, 403, 420, 661, 713, 821
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 311, 612
NspI RCATGY 1 cut(s) 209
NspV TTCGAA 1 cut(s) 110
PaeR7I CTCGAG 1 cut(s) 179
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 781
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 3 cut(s) 37, 112, 278
Pfl23II CGTACG 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 5 cut(s) 60, 100, 739, 824, 977
PleI GAGTC 2 cut(s) 218, 544
PpsI GAGTC 2 cut(s) 218, 544
PpuMI RGGWCCY 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 815
PspEI GGTNACC 1 cut(s) 612
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 1 cut(s) 815
PspLI CGTACG 1 cut(s) 51
PspN4I GGNNCC 7 cut(s) 47, 48, 403, 420, 661, 713, 821
PspPI GGNCC 1 cut(s) 46
PspPPI RGGWCCY 1 cut(s) 46
PspXI VCTCGAGB 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 148
PsuI RGATCY 5 cut(s) 24, 64, 594, 921, 943
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 3 cut(s) 53, 713, 778
RsaNI GTAC 3 cut(s) 52, 712, 777
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 2 cut(s) 210, 336
SatI GCNGC 5 cut(s) 59, 99, 738, 823, 976
Sau3AI GATC 7 cut(s) 24, 64, 78, 409, 594, 921, 943
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 2 cut(s) 218, 544
ScrFI CCNGG 4 cut(s) 42, 817, 892, 893
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 179
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 179
SmaI CCCGGG 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 179, 520
SmoI CTYRAG 2 cut(s) 179, 520
Sse9I AATT 5 cut(s) 11, 237, 370, 694, 786
SsiI CCGC 6 cut(s) 32, 98, 122, 229, 823, 976
SspI AATATT 1 cut(s) 473
SspMI CTAG 3 cut(s) 102, 245, 988
StyD4I CCNGG 4 cut(s) 40, 815, 890, 891
TaaI ACNGT 2 cut(s) 391, 768
TaiI ACGT 1 cut(s) 86
TaqI TCGA 5 cut(s) 86, 110, 180, 602, 784
TasI AATT 5 cut(s) 11, 237, 370, 694, 786
TauI GCSGC 3 cut(s) 101, 825, 978
TfiI GAWTC 3 cut(s) 37, 112, 278
Tru1I TTAA 2 cut(s) 210, 336
Tru9I TTAA 2 cut(s) 210, 336
TscAI CASTG 4 cut(s) 288, 705, 771, 850
TseFI GTSAC 2 cut(s) 311, 612
TseI GCWGC 2 cut(s) 58, 737
Tsp45I GTSAC 2 cut(s) 311, 612
TspDTI ATGAA 3 cut(s) 363, 528, 696
TspGWI ACGGA 1 cut(s) 706
TspMI CCCGGG 1 cut(s) 891
TspRI CASTG 4 cut(s) 288, 705, 771, 850
Van91I CCANNNNNTGG 1 cut(s) 755
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 694
XceI RCATGY 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 179
XmaI CCCGGG 1 cut(s) 891
XspI CTAG 3 cut(s) 102, 245, 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.