pycom05g20500

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012031
Physical Location & Seq
Reverse (-)
23286156 .. 23290342
4187 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20500.10

Sequence Viewer

Length: 1386 bp
ATGCTCTTATGGGACAAAAAATGTGTTTTGGTGGGCCTTTATTGCAAAATGGGGGTTTGTTTTGGGCTCAAATACGATTTGAGCTTTATATGTTTCAGGCCTAATATTTGTTTGGATTTTGGAGGGAAACCCACGCAATTTTCGAAAAAAAGACGGCGACCCCGGAAAAAGGGAAAGGCCAGAGAGTGGGAATTTATTCAAATGAAACTTATTGAAATTAAAAAAATAAATAATAATTGTCGTTGTGCCCAATGTGATGTTCGAAGAAGGAAAATAATACAATCGAATCAAACCAACGGCCACAATCACTGCGCGGAGACTCGAAGCTCGGAGGAGGAGTCAGCTATGTCGCAGGGCAGTGGAAGCGTCGGTGGTGGTGGAGGTGGCGGTGGGCTCGACTTTCACCTGCCGGACGGGATCTTGTCGGTGATACCGAGCGACCCGTACGACCAGCTGGATCTGGCGCGGAAGATCACGTCGATGGCCATAGCGTCTAGGGTTTCGCAGCTGGAGTCTGAGATGGGGAAGATGAGGCAGAAGCTGCAGGACAAAGATAGGGTTGTGTTTGAGCTCGAGGAAAGGGTCTCGCGCCTCCAGCACGCCAACCACGAGGCTGAGTCCCGCCTGAGAATTTCTCTGGATGAGAATTTGAAGCTTTCCAAAGAGCGAGATTCGTTGGCATTGACTACTAAAAAGCTCAGCCGTGACTTATCAAAGTTGGAGACATTTAAGAGACAACTAATGCAGTCGCTAAATGATGATAATGGATCTCCAGCTGAAACTGTTGATATTGGCACTGTTCCAAAGGCATATCCTGATAAGGATGAGGAGACAAATGGCTACATAGGACAATATTCCTCAAGTGGTTCCACAGATAAGGGAGCAACTGATGAAGTCTTGAAGAATTCCAGTCAAGGATTTTCTATAACTCCATATATCACTCCGCGGCTTACTCCAACTGGAACTCCAAAGATTATTTCCACAAGTGGGTCACCTAGAGGGTATTCTGCCATTTCTTCTCCTCAGCAATCATCTGGTGCCACATCTCCCCAAAAATCCGCCTATAAAGAACGGACTTCATTCTCTTCATGGTACTCATCAAGCCAGCAGTCATCAGCAGCAAACTCTCCACCTCGTGGACGTTCACATTCAGGGCGCACTCCTCGAATTGACGGAAAAGAGTTCTTTCGTCAGGCCAGGAGTCGTTTATCATATGAGCAGTTCAGTGCATTTCTGGCTAAAATCAAGGAACGAAATGCTCATAAGCAAACCAGAGAGATAATAATTGATCCCTTCGTTAGGCTGTGGGTCGAGCTTGTTCTTGGCTGCTTTGGTCCTTTGGATGGAAGGTTCATGGCTTGTCCTCTGTGGCATATCATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

51.64

Weight (kDa)

9.48

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16520
fragaria_vesca FvH4_3g15540
malus_domestica MD05G1222300.v1.1 MD10G1203900.v1.1
prunus_persica Prupe.4G138000_v2.0.a1 Prupe.4G138000_v2.0.a1
pyrus_communis pycom05g20500 pycom10g17450
rosa_chinensis RchiOBHm_Chr5g0026171
rosa_laevigata RLG00000032909
rosa_multiflora Rmu_sc0002909.1_g000014
rosa_roxburghii Rroxscaffold_1G00053140
rosa_rugosa Rorug05G0091500.1 Rorug05G0091600
rosa_samantha Rh5AG184200 Rh5BG181000 Rh5CG200000 Rh5DG182600
rosa_wichuraiana Rw5G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 1037
AccB7I CCANNNNNTGG 2 cut(s) 186, 1136
AccII CGCG 4 cut(s) 314, 466, 589, 946
AciI CCGC 7 cut(s) 314, 387, 466, 622, 944, 946, 1059
AclWI GGATC 4 cut(s) 425, 465, 775, 1283
AcoI YGGCCR 2 cut(s) 298, 483
AcsI RAATTY 4 cut(s) 191, 630, 646, 904
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 2 cut(s) 446, 1094
AfiI CCNNNNNNNGG 6 cut(s) 169, 186, 987, 1136, 1299, 1343
AgsI TTSAA 4 cut(s) 200, 215, 652, 901
AjiI CACGTC 1 cut(s) 477
AjnI CCWGG 1 cut(s) 1196
AjuI GAANNNNNNNTTGG 4 cut(s) 243, 275, 961, 993
AloI GAACNNNNNNTCC 2 cut(s) 1334, 1366
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 5 cut(s) 311, 589, 716, 727, 824
AlwI GGATC 4 cut(s) 425, 465, 775, 1283
AlwNI CAGNNNCTG 1 cut(s) 541
Ama87I CYCGRG 1 cut(s) 572
AoxI GGCC 6 cut(s) 34, 98, 177, 298, 483, 1194
ApeKI GCWGC 4 cut(s) 505, 541, 1118, 1326
ApoI RAATTY 4 cut(s) 191, 630, 646, 904
Asp700I GAANNNNTTC 1 cut(s) 195
AspLEI GCGC 4 cut(s) 314, 466, 591, 1158
AspS9I GGNCC 2 cut(s) 34, 1334
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 395, 439, 984
AsuII TTCGAA 2 cut(s) 143, 262
AvaI CYCGRG 1 cut(s) 572
AvaII GGWCC 1 cut(s) 1334
BaeGI GKGCMC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 485
BanI GGYRCC 1 cut(s) 1037
BanII GRGCYC 3 cut(s) 69, 396, 573
BauI CACGAG 2 cut(s) 608, 1134
Bbv12I GWGCWC 1 cut(s) 573
BbvCI CCTCAGC 1 cut(s) 1023
BbvI GCAGC 4 cut(s) 517, 528, 1130, 1313
BccI CCATC 3 cut(s) 475, 514, 1337
BceAI ACGGC 3 cut(s) 170, 313, 687
BciT130I CCWGG 1 cut(s) 1198
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 5 cut(s) 311, 589, 716, 727, 824
BfaI CTAG 2 cut(s) 495, 996
BfmI CTRYAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 414
BisI GCNGC 5 cut(s) 506, 542, 947, 1119, 1327
BlpI GCTNAGC 1 cut(s) 698
BlsI GCNGC 5 cut(s) 507, 543, 948, 1120, 1328
Bme1390I CCNGG 2 cut(s) 163, 1198
Bme18I GGWCC 1 cut(s) 1334
BmeT110I CYCGRG 1 cut(s) 572
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 34, 1334
BmiI GGNNCC 2 cut(s) 868, 1039
BmrFI CCNGG 2 cut(s) 163, 1198
BplI GAGNNNNNCTC 2 cut(s) 619, 651
BpmI CTGGAG 3 cut(s) 530, 578, 756
Bpu10I CCTNAGC 1 cut(s) 1023
Bpu1102I GCTNAGC 1 cut(s) 698
Bpu14I TTCGAA 2 cut(s) 143, 262
BpuEI CTTGAG 1 cut(s) 844
BpuMI CCSGG 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 589
BsaJI CCNNGG 2 cut(s) 161, 944
BsaXI ACNNNNNCTCC 4 cut(s) 323, 353, 949, 979
Bsc4I CCNNNNNNNGG 6 cut(s) 169, 186, 987, 1136, 1299, 1343
Bse1I ACTGG 2 cut(s) 909, 964
BseBI CCWGG 1 cut(s) 1198
BseDI CCNNGG 2 cut(s) 161, 944
BseGI GGATG 3 cut(s) 646, 829, 1348
BseLI CCNNNNNNNGG 6 cut(s) 169, 186, 987, 1136, 1299, 1343
BseMII CTCAG 5 cut(s) 507, 606, 617, 712, 1037
BseNI ACTGG 2 cut(s) 909, 964
BseRI GAGGAG 5 cut(s) 347, 350, 842, 1011, 1152
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 4 cut(s) 517, 528, 1130, 1313
Bsh1236I CGCG 4 cut(s) 314, 466, 589, 946
BshFI GGCC 6 cut(s) 36, 100, 179, 300, 485, 1196
BshNI GGYRCC 1 cut(s) 1037
BsiHKAI GWGCWC 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 572
BsiSI CCGG 2 cut(s) 163, 410
BsiWI CGTACG 1 cut(s) 444
BslFI GGGAC 2 cut(s) 26, 604
BslI CCNNNNNNNGG 6 cut(s) 169, 186, 987, 1136, 1299, 1343
BsmAI GTCTC 5 cut(s) 311, 589, 716, 727, 824
BsmFI GGGAC 2 cut(s) 26, 604
BsnI GGCC 6 cut(s) 36, 100, 179, 300, 485, 1196
Bso31I GGTCTC 1 cut(s) 589
BsoBI CYCGRG 1 cut(s) 572
Bsp119I TTCGAA 2 cut(s) 143, 262
Bsp1286I GDGCHC 4 cut(s) 69, 250, 396, 573
Bsp143I GATC 5 cut(s) 417, 457, 471, 767, 1288
Bsp1720I GCTNAGC 1 cut(s) 698
BspACI CCGC 7 cut(s) 314, 387, 466, 622, 944, 946, 1059
BspANI GGCC 6 cut(s) 36, 100, 179, 300, 485, 1196
BspCNI CTCAG 5 cut(s) 508, 607, 618, 711, 1036
BspFNI CGCG 4 cut(s) 314, 466, 589, 946
BspLI GGNNCC 2 cut(s) 868, 1039
BspMAI CTGCAG 1 cut(s) 546
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 4 cut(s) 425, 465, 775, 1283
BspT104I TTCGAA 2 cut(s) 143, 262
BspT107I GGYRCC 1 cut(s) 1037
BspTNI GGTCTC 1 cut(s) 589
BsrI ACTGG 2 cut(s) 909, 964
BssECI CCNNGG 2 cut(s) 161, 944
BssMI GATC 5 cut(s) 417, 457, 471, 767, 1288
BssSI CACGAG 2 cut(s) 608, 1134
Bst2BI CACGAG 2 cut(s) 608, 1134
Bst2UI CCWGG 1 cut(s) 1198
Bst4CI ACNGT 2 cut(s) 784, 799
Bst6I CTCTTC 1 cut(s) 1090
BstAPI GCANNNNNTGC 1 cut(s) 541
BstBI TTCGAA 2 cut(s) 143, 262
BstC8I GCNNGC 2 cut(s) 600, 1106
BstDEI CTNAG 5 cut(s) 516, 615, 626, 698, 1023
BstDSI CCRYGG 1 cut(s) 944
BstEII GGTNACC 1 cut(s) 990
BstENI CCTNNNNNAGG 1 cut(s) 1297
BstF5I GGATG 3 cut(s) 646, 829, 1348
BstFNI CGCG 4 cut(s) 314, 466, 589, 946
BstHHI GCGC 4 cut(s) 314, 466, 591, 1158
BstKTI GATC 5 cut(s) 420, 460, 474, 770, 1291
BstMAI GTCTC 5 cut(s) 311, 589, 716, 727, 824
BstMBI GATC 5 cut(s) 417, 457, 471, 767, 1288
BstMWI GCNNNNNNNGC 5 cut(s) 42, 363, 541, 595, 1235
BstNI CCWGG 1 cut(s) 1198
BstPI GGTNACC 1 cut(s) 990
BstSCI CCNGG 2 cut(s) 161, 1196
BstSFI CTRYAG 1 cut(s) 542
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 4 cut(s) 314, 466, 589, 946
BstV1I GCAGC 4 cut(s) 517, 528, 1130, 1313
BstX2I RGATCY 3 cut(s) 417, 457, 767
BstYI RGATCY 3 cut(s) 417, 457, 767
BsuRI GGCC 6 cut(s) 36, 100, 179, 300, 485, 1196
BtgI CCRYGG 1 cut(s) 944
BtrI CACGTC 1 cut(s) 477
BtsCI GGATG 3 cut(s) 646, 829, 1348
BtsI GCAGTG 2 cut(s) 307, 364
BtsIMutI CAGTG 4 cut(s) 307, 364, 795, 1231
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 600, 1106
CaiI CAGNNNCTG 1 cut(s) 541
CfoI GCGC 4 cut(s) 314, 466, 591, 1158
Cfr13I GGNCC 2 cut(s) 34, 1334
Cfr42I CCGCGG 1 cut(s) 947
CseI GACGC 2 cut(s) 355, 480
Csp6I GTAC 2 cut(s) 445, 1093
CviAII CATG 2 cut(s) 1089, 1354
CviQI GTAC 2 cut(s) 445, 1093
DdeI CTNAG 5 cut(s) 516, 615, 626, 698, 1023
DpnI GATC 5 cut(s) 419, 459, 473, 769, 1290
DpnII GATC 5 cut(s) 417, 457, 471, 767, 1288
DraIII CACNNNGTG 1 cut(s) 1136
EaeI YGGCCR 2 cut(s) 298, 483
Eam1104I CTCTTC 1 cut(s) 1090
EarI CTCTTC 1 cut(s) 1090
EciI GGCGGA 1 cut(s) 1048
Ecl136II GAGCTC 1 cut(s) 571
Eco147I AGGCCT 1 cut(s) 100
Eco24I GRGCYC 3 cut(s) 69, 396, 573
Eco31I GGTCTC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 1334
Eco53kI GAGCTC 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 572
Eco91I GGTNACC 1 cut(s) 990
EcoICRI GAGCTC 1 cut(s) 571
EcoNI CCTNNNNNAGG 1 cut(s) 1297
EcoO65I GGTNACC 1 cut(s) 990
EcoRI GAATTC 1 cut(s) 904
EcoRII CCWGG 1 cut(s) 1196
EcoT38I GRGCYC 3 cut(s) 69, 396, 573
FaeI CATG 2 cut(s) 1092, 1357
FaqI GGGAC 2 cut(s) 26, 604
FatI CATG 2 cut(s) 1088, 1353
FauI CCCGC 1 cut(s) 629
FauNDI CATATG 1 cut(s) 1213
Fnu4HI GCNGC 5 cut(s) 506, 542, 947, 1119, 1327
FokI GGATG 3 cut(s) 653, 836, 1355
FriOI GRGCYC 3 cut(s) 69, 396, 573
Fsp4HI GCNGC 5 cut(s) 506, 542, 947, 1119, 1327
FspBI CTAG 2 cut(s) 495, 996
GlaI GCGC 4 cut(s) 313, 465, 590, 1157
GluI GCNGC 5 cut(s) 506, 542, 947, 1119, 1327
GsuI CTGGAG 3 cut(s) 530, 578, 756
HaeIII GGCC 6 cut(s) 36, 100, 179, 300, 485, 1196
HapII CCGG 2 cut(s) 163, 410
HgaI GACGC 2 cut(s) 355, 480
HhaI GCGC 4 cut(s) 314, 466, 591, 1158
Hin1II CATG 2 cut(s) 1092, 1357
Hin6I GCGC 4 cut(s) 312, 464, 589, 1156
HinP1I GCGC 4 cut(s) 312, 464, 589, 1156
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 7 cut(s) 286, 319, 338, 512, 617, 671, 1201
HpaII CCGG 2 cut(s) 163, 410
HphI GGTGA 3 cut(s) 395, 439, 984
Hpy166II GTNNAC 2 cut(s) 1139, 1145
Hpy188I TCNGA 2 cut(s) 331, 517
Hpy188III TCNNGA 3 cut(s) 638, 815, 898
Hpy8I GTNNAC 2 cut(s) 1139, 1145
Hpy99I CGWCG 2 cut(s) 371, 481
HpyAV CCTTC 3 cut(s) 261, 1303, 1341
HpyCH4III ACNGT 2 cut(s) 784, 799
HpyCH4IV ACGT 2 cut(s) 476, 1141
HpyCH4V TGCA 4 cut(s) 45, 544, 745, 1229
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 363, 541, 595, 1235
HpyF3I CTNAG 5 cut(s) 516, 615, 626, 698, 1023
HpySE526I ACGT 2 cut(s) 476, 1141
Hsp92II CATG 2 cut(s) 1092, 1357
HspAI GCGC 4 cut(s) 312, 464, 589, 1156
KspI CCGCGG 1 cut(s) 947
Kzo9I GATC 5 cut(s) 417, 457, 471, 767, 1288
LmnI GCTCC 1 cut(s) 881
Lsp1109I GCAGC 4 cut(s) 517, 528, 1130, 1313
MaeI CTAG 2 cut(s) 495, 996
MaeII ACGT 2 cut(s) 476, 1141
MaeIII GTNAC 2 cut(s) 704, 990
MalI GATC 5 cut(s) 419, 459, 473, 769, 1290
MboI GATC 5 cut(s) 417, 457, 471, 767, 1288
MboII GAAGA 6 cut(s) 276, 481, 538, 913, 1008, 1077
MflI RGATCY 3 cut(s) 417, 457, 767
MhlI GDGCHC 4 cut(s) 69, 250, 396, 573
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 9 cut(s) 137, 191, 216, 235, 630, 646, 904, 1167, 1284
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 5 cut(s) 313, 347, 521, 626, 1210
MmeI TCCRAC 2 cut(s) 699, 980
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 195
MscI TGGCCA 1 cut(s) 485
MseI TTAA 2 cut(s) 219, 729
MslI CAYNNNNRTG 1 cut(s) 479
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 4 cut(s) 454, 508, 776, 946
MspI CCGG 2 cut(s) 163, 410
MspR9I CCNGG 2 cut(s) 163, 1198
MvaI CCWGG 1 cut(s) 1198
MvnI CGCG 4 cut(s) 314, 466, 589, 946
MwoI GCNNNNNNNGC 5 cut(s) 42, 363, 541, 595, 1235
NciI CCSGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 1213
NdeII GATC 5 cut(s) 417, 457, 471, 767, 1288
NlaIII CATG 2 cut(s) 1092, 1357
NlaIV GGNNCC 2 cut(s) 868, 1039
NmuCI GTSAC 2 cut(s) 704, 990
NspV TTCGAA 2 cut(s) 143, 262
PaeR7I CTCGAG 1 cut(s) 572
PaqCI CACCTGC 1 cut(s) 414
PceI AGGCCT 1 cut(s) 100
PcsI WCGNNNNNNNCGW 3 cut(s) 140, 431, 606
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 286, 671
Pfl23II CGTACG 1 cut(s) 444
PflMI CCANNNNNTGG 2 cut(s) 186, 1136
PkrI GCNGC 5 cut(s) 507, 543, 948, 1120, 1328
PleI GAGTC 5 cut(s) 313, 346, 520, 625, 1209
PpsI GAGTC 5 cut(s) 313, 346, 520, 625, 1209
Psp124BI GAGCTC 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 1196
PspEI GGTNACC 1 cut(s) 990
PspGI CCWGG 1 cut(s) 1196
PspLI CGTACG 1 cut(s) 444
PspN4I GGNNCC 2 cut(s) 868, 1039
PspPI GGNCC 2 cut(s) 34, 1334
PspXI VCTCGAGB 1 cut(s) 572
PstI CTGCAG 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 541
PsuI RGATCY 3 cut(s) 417, 457, 767
PvuII CAGCTG 3 cut(s) 454, 508, 776
RsaI GTAC 2 cut(s) 446, 1094
RsaNI GTAC 2 cut(s) 445, 1093
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 573
SacII CCGCGG 1 cut(s) 947
SaqAI TTAA 2 cut(s) 219, 729
SatI GCNGC 5 cut(s) 506, 542, 947, 1119, 1327
Sau3AI GATC 5 cut(s) 417, 457, 471, 767, 1288
Sau96I GGNCC 2 cut(s) 34, 1334
SchI GAGTC 5 cut(s) 313, 347, 521, 626, 1210
ScrFI CCNGG 2 cut(s) 163, 1198
SduI GDGCHC 4 cut(s) 69, 250, 396, 573
SfcI CTRYAG 1 cut(s) 542
Sfr274I CTCGAG 1 cut(s) 572
Sfr303I CCGCGG 1 cut(s) 947
SfuI TTCGAA 2 cut(s) 143, 262
SgrBI CCGCGG 1 cut(s) 947
SinI GGWCC 1 cut(s) 1334
SlaI CTCGAG 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 2 cut(s) 572, 859
SmoI CTYRAG 2 cut(s) 572, 859
Sse9I AATT 9 cut(s) 137, 191, 216, 235, 630, 646, 904, 1167, 1284
SseBI AGGCCT 1 cut(s) 100
SsiI CCGC 7 cut(s) 314, 387, 466, 622, 944, 946, 1059
SspI AATATT 2 cut(s) 106, 854
SspMI CTAG 2 cut(s) 495, 996
SstI GAGCTC 1 cut(s) 573
StuI AGGCCT 1 cut(s) 100
StyD4I CCNGG 2 cut(s) 161, 1196
TaaI ACNGT 2 cut(s) 784, 799
TaiI ACGT 2 cut(s) 479, 1144
TaqI TCGA 9 cut(s) 143, 262, 284, 322, 396, 479, 573, 1165, 1311
TasI AATT 9 cut(s) 137, 191, 216, 235, 630, 646, 904, 1167, 1284
TauI GCSGC 1 cut(s) 949
TfiI GAWTC 2 cut(s) 286, 671
Tru1I TTAA 2 cut(s) 219, 729
Tru9I TTAA 2 cut(s) 219, 729
TscAI CASTG 4 cut(s) 314, 364, 802, 1231
TseFI GTSAC 2 cut(s) 704, 990
TseI GCWGC 4 cut(s) 505, 541, 1118, 1326
Tsp45I GTSAC 2 cut(s) 704, 990
TspDTI ATGAA 5 cut(s) 218, 906, 1068, 1077, 1342
TspGWI ACGGA 2 cut(s) 1087, 1188
TspRI CASTG 4 cut(s) 314, 364, 802, 1231
Van91I CCANNNNNTGG 2 cut(s) 186, 1136
VpaK11BI GGWCC 1 cut(s) 1334
XagI CCTNNNNNAGG 1 cut(s) 1297
XapI RAATTY 4 cut(s) 191, 630, 646, 904
XhoI CTCGAG 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 2 cut(s) 495, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.