AT1G16700

NADH dehydrogenase ubiquinone iron-sulfur protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012371
Physical Location & Seq
Forward (+)
5709624 .. 5712043
2420 bp
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UTR
Exon/CDS
Intron
AT1G16700.1

Sequence Viewer

Length: 669 bp
ATGGCTTCGCTACTAGCTCGCAGGTCGTTCAGTGCTCTCCGAGCTCGACATCTCGCTTTTTCAGGGCAAGGTTTACAGGGATCTCATCTCTGTGGGCTACAGTCCCGTGCTATATCTTATGGAAGCAACAAAGATGATGAAGAAGCTGAGCAGCTTGCTAAAGAGATCTCCAAGGACTGGAGTACTGTCTTTGAACGGAGCATGAACACCCTATTTCTCACTGAAATGGTCAGGGGTTTGTCACTGACCCTCAAGTACTTCTTTGATCCAAAAGTTACAATCAATTATCCTTTTGAGAAGGGTCCATTGAGCCCTCGTTTCCGTGGTGAACACGCTCTTCGAAGGTATCCAACTGGGGAAGAACGCTGCATTGCCTGCAAACTCTGCGAAGCTGTATGTCCAGCTCAAGCAATCACAATAGAGGCAGAGGAGCGGGAAGATGGAAGCCGCAGAACCACTAGGTATGATATCGACATGACGAAATGCATCTATTGTGGTTTCTGCCAAGAAGCTTGCCCTGTTGATGCAATCGTGGAAGGACCAAACTTTGAGTTTGCAACCGAGACACATGAGGAACTTCTGTATGACAAAGAGAAGCTTCTTGAGAATGGAGATAGGTGGGAGACAGAGATTGCAGAGAATCTGAGGTCGGAGAGCCTCTACCGCTGA

Protein Analysis

222

Amino Acids

25.38

Weight (kDa)

5.22

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer4 PF00037 120 - 139 5.3e-06 4Fe-4S binding domain
Fer4_10 PF13237 120 - 173 3.7e-06 4Fe-4S dicluster domain
Fer4_7 PF12838 122 - 176 6.4e-13 4Fe-4S dicluster domain
Fer4_9 PF13187 122 - 177 6.5e-08 4Fe-4S dicluster domain
Fer4 PF00037 156 - 177 2.4e-08 4Fe-4S binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16700 AT1G79010
fragaria_vesca FvH4_5g29280
malus_domestica MD05G1066300.v1.1 MD10G1076300.v1.1
prunus_persica Prupe.8G096900_v2.0.a1 Prupe.8G096900_v2.0.a1
pyrus_communis pycom05g05750 pycom10g05970
rosa_chinensis RchiOBHm_Chr7g0222141
rosa_laevigata RLG00000002089
rosa_multiflora Rmu_sc0006951.1_g000002
rosa_roxburghii Rroxscaffold_3G00237380
rosa_rugosa Rorug07G0205700
rosa_samantha Rh7AG346900 Rh7BG336800 Rh7CG364200 Rh7DG344900
rosa_wichuraiana Rw7G029380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 12
AccB7I CCANNNNNTGG 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 433
AciI CCGC 3 cut(s) 433, 448, 664
AclWI GGATC 2 cut(s) 88, 260
AfaI GTAC 2 cut(s) 184, 257
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 8 cut(s) 17, 44, 146, 154, 392, 404, 512, 598
AluI AGCT 8 cut(s) 17, 44, 146, 154, 392, 404, 512, 598
Alw21I GWGCWC 2 cut(s) 37, 46
Alw26I GTCTC 2 cut(s) 557, 617
AlwI GGATC 2 cut(s) 88, 260
ApeKI GCWGC 2 cut(s) 151, 366
AspS9I GGNCC 2 cut(s) 302, 539
AsuHPI GGTGA 1 cut(s) 338
AsuII TTCGAA 1 cut(s) 340
AvaII GGWCC 2 cut(s) 302, 539
BanII GRGCYC 2 cut(s) 46, 314
Bbv12I GWGCWC 2 cut(s) 37, 46
BbvI GCAGC 2 cut(s) 163, 353
BccI CCATC 1 cut(s) 434
BciVI GTATCC 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 557, 617
BfaI CTAG 2 cut(s) 14, 459
BfmI CTRYAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 12
BfuI GTATCC 1 cut(s) 357
BglII AGATCT 1 cut(s) 165
BisI GCNGC 3 cut(s) 152, 367, 448
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 3 cut(s) 153, 368, 449
BmcAI AGTACT 2 cut(s) 184, 257
Bme18I GGWCC 2 cut(s) 302, 539
BmgT120I GGNCC 2 cut(s) 302, 539
BmiI GGNNCC 1 cut(s) 303
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 495, 514
BmuI ACTGGG 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 199
Bpu1102I GCTNAGC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 340
BpuEI CTTGAG 3 cut(s) 236, 390, 623
BsaJI CCNNGG 2 cut(s) 171, 322
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 182, 358
Bse3DI GCAATG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 171, 322
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 138, 635
BseNI ACTGG 2 cut(s) 182, 358
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 2 cut(s) 163, 353
BsiHKAI GWGCWC 2 cut(s) 37, 46
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 2 cut(s) 557, 617
BsmFI GGGAC 1 cut(s) 88
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 3 cut(s) 37, 46, 314
Bsp143I GATC 3 cut(s) 80, 165, 265
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 3 cut(s) 433, 448, 664
BspCNI CTCAG 2 cut(s) 139, 636
BspLI GGNNCC 1 cut(s) 303
BspMI ACCTGC 1 cut(s) 12
BspPI GGATC 2 cut(s) 88, 260
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 340
BsrBI CCGCTC 1 cut(s) 433
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 2 cut(s) 182, 358
BssECI CCNNGG 2 cut(s) 171, 322
BssMI GATC 3 cut(s) 80, 165, 265
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 102, 187
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 2 cut(s) 375, 384
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 4 cut(s) 19, 156, 376, 514
BstDEI CTNAG 2 cut(s) 147, 644
BstDSI CCRYGG 1 cut(s) 322
BstKTI GATC 3 cut(s) 83, 168, 268
BstMAI GTCTC 2 cut(s) 557, 617
BstMBI GATC 3 cut(s) 80, 165, 265
BstMWI GCNNNNNNNGC 4 cut(s) 41, 375, 384, 663
BstSFI CTRYAG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 163, 353
BstX2I RGATCY 2 cut(s) 80, 165
BstYI RGATCY 2 cut(s) 80, 165
BsuI GTATCC 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 322
BtsIMutI CAGTG 3 cut(s) 37, 219, 242
BveI ACCTGC 1 cut(s) 12
Cac8I GCNNGC 4 cut(s) 19, 156, 376, 514
Cfr13I GGNCC 2 cut(s) 302, 539
Csp6I GTAC 2 cut(s) 183, 256
CviAII CATG 3 cut(s) 202, 475, 569
CviQI GTAC 2 cut(s) 183, 256
DdeI CTNAG 2 cut(s) 147, 644
DpnI GATC 3 cut(s) 82, 167, 267
DpnII GATC 3 cut(s) 80, 165, 265
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 2 cut(s) 46, 314
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 2 cut(s) 302, 539
Eco53kI GAGCTC 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 44
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 171
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 2 cut(s) 46, 314
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 3 cut(s) 205, 478, 572
FaiI YATR 8 cut(s) 113, 120, 203, 397, 465, 476, 570, 585
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 582, 614
FaqI GGGAC 1 cut(s) 88
FatI CATG 3 cut(s) 201, 474, 568
FauI CCCGC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 152, 367, 448
FriOI GRGCYC 2 cut(s) 46, 314
Fsp4HI GCNGC 3 cut(s) 152, 367, 448
FspBI CTAG 2 cut(s) 14, 459
GluI GCNGC 3 cut(s) 152, 367, 448
GsuI CTGGAG 1 cut(s) 199
Hin1II CATG 3 cut(s) 205, 478, 572
HindIII AAGCTT 2 cut(s) 510, 596
HinfI GANTC 1 cut(s) 640
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 2 cut(s) 74, 329
Hpy188I TCNGA 3 cut(s) 41, 645, 652
Hpy188III TCNNGA 1 cut(s) 602
Hpy8I GTNNAC 2 cut(s) 74, 329
HpyAV CCTTC 3 cut(s) 292, 336, 530
HpyCH4III ACNGT 2 cut(s) 102, 187
HpyCH4V TGCA 6 cut(s) 369, 378, 486, 527, 557, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 375, 384, 663
HpyF3I CTNAG 2 cut(s) 147, 644
Hsp92II CATG 3 cut(s) 205, 478, 572
Kzo9I GATC 3 cut(s) 80, 165, 265
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 198, 430
LpnPI CCDG 9 cut(s) 7, 48, 62, 163, 217, 339, 388, 414, 531
Lsp1109I GCAGC 2 cut(s) 163, 353
LweI GCATC 2 cut(s) 495, 514
MaeI CTAG 2 cut(s) 14, 459
MaeIII GTNAC 2 cut(s) 240, 274
MalI GATC 3 cut(s) 82, 167, 267
MbiI CCGCTC 1 cut(s) 433
MboI GATC 3 cut(s) 80, 165, 265
MboII GAAGA 4 cut(s) 152, 329, 371, 449
MflI RGATCY 2 cut(s) 80, 165
MhlI GDGCHC 3 cut(s) 37, 46, 314
MluCI AATT 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 374, 630
MnlI CCTC 7 cut(s) 260, 324, 415, 421, 565, 639, 668
Mph1103I ATGCAT 1 cut(s) 488
MslI CAYNNNNRTG 2 cut(s) 90, 224
MspA1I CMGCKG 1 cut(s) 666
MwoI GCNNNNNNNGC 4 cut(s) 41, 375, 384, 663
NdeII GATC 3 cut(s) 80, 165, 265
NlaIII CATG 3 cut(s) 205, 478, 572
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 488
NspV TTCGAA 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 640
PflMI CCANNNNNTGG 1 cut(s) 177
PkrI GCNGC 3 cut(s) 153, 368, 449
Psp124BI GAGCTC 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 2 cut(s) 302, 539
PsuI RGATCY 2 cut(s) 80, 165
RsaI GTAC 2 cut(s) 184, 257
RsaNI GTAC 2 cut(s) 183, 256
RseI CAYNNNNRTG 2 cut(s) 90, 224
SacI GAGCTC 1 cut(s) 46
SapI GCTCTTC 1 cut(s) 342
SatI GCNGC 3 cut(s) 152, 367, 448
Sau3AI GATC 3 cut(s) 80, 165, 265
Sau96I GGNCC 2 cut(s) 302, 539
ScaI AGTACT 2 cut(s) 184, 257
SduI GDGCHC 3 cut(s) 37, 46, 314
SfaNI GCATC 2 cut(s) 495, 514
SfcI CTRYAG 1 cut(s) 98
SfuI TTCGAA 1 cut(s) 340
SinI GGWCC 2 cut(s) 302, 539
SmiMI CAYNNNNRTG 2 cut(s) 90, 224
SmlI CTYRAG 3 cut(s) 251, 405, 602
SmoI CTYRAG 3 cut(s) 251, 405, 602
Sse9I AATT 1 cut(s) 283
SsiI CCGC 3 cut(s) 433, 448, 664
SspMI CTAG 2 cut(s) 14, 459
SstI GAGCTC 1 cut(s) 46
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 102, 187
TaqI TCGA 3 cut(s) 46, 340, 471
TasI AATT 1 cut(s) 283
TatI WGTACW 2 cut(s) 182, 255
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 1 cut(s) 640
TscAI CASTG 3 cut(s) 37, 226, 249
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 2 cut(s) 151, 366
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 2 cut(s) 153, 218
TspGWI ACGGA 2 cut(s) 211, 311
TspRI CASTG 3 cut(s) 37, 226, 249
Van91I CCANNNNNTGG 1 cut(s) 177
VpaK11BI GGWCC 2 cut(s) 302, 539
XspI CTAG 2 cut(s) 14, 459
ZrmI AGTACT 2 cut(s) 184, 257
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.