Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012371

Physical Location & Seq

Genomic Coordinates Reverse (-)

12428611 .. 12432706

Gene Structure 4096 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1066300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGGCTGCGATCTTAGCTCGGAAATCTCTCCAGGCTCTCCGCGCCAGACAGCTCGTTGTACCAGGCCAAGCATTGCAGAGTTCTTCCTACTATGGACTGCAATCCAGTTCACAACACTTCTCAACCATAAAAGAGGATGAGGAAAGAGAGCAACTCGCAAAGGAGATTTCCAAGGACTGGAGTACTGTTTTTGATCGAAGCATAAACACATTGTTCCTCACTGAAATGGTTCGAGGTCTGATGCTTACGCTTAAGTACTTTTTTGAGAAAAAAGTTACTATCAATTATCCTTTTGAGAAGGGCCCTTTGAGTCCTCGTTTTCGTGGTGAACATGCCCTACGGCGATATCCTACGGGAGAAGAGCGTTGCATTGCATGCAAGCTTTGTGAAGCTATCTGTCCTGCACAAGCAATAACAATTGAGGCTGAGGAACGAGAAGATGGTAGCCGTAGGACTACAAGGTATGACATTGACATGACAAAGTGCATCTACTGTGGATTCTGTCAAGAGGCATGTCCCGTTGATGCCATTGTCGAAGGACCAAACTTTGAATTCTCAACAGAGACTCATGAGGAGCTTCTTTACGACAAGGAAAAGCTGCTTGAGAATGGAGATCGTTGGGAGACCGAGATTGCTGAGAACCTCAGATCCGAAAGCCTTTACCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005747 GO:0016020 GO:0019866 GO:0030964 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045271 GO:0046872 GO:0070469 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016

Pfam Domains

Protein Families

Fer4

Protein Analysis

223

Amino Acids

25.65

Weight (kDa)

5.24

Isoelectric Point (pI)

61.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer4_7	PF12838	122 - 176	1.3e-12	4Fe-4S dicluster domain
Fer4_9	PF13187	122 - 177	1.2e-07	4Fe-4S dicluster domain
Fer4	PF00037	156 - 177	2.4e-08	4Fe-4S binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012371)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16700 AT1G79010
fragaria_vesca	FvH4_5g29280
malus_domestica	MD05G1066300.v1.1 MD10G1076300.v1.1
prunus_persica	Prupe.8G096900_v2.0.a1 Prupe.8G096900_v2.0.a1
pyrus_communis	pycom05g05750 pycom10g05970
rosa_chinensis	RchiOBHm_Chr7g0222141
rosa_laevigata	RLG00000002089
rosa_multiflora	Rmu_sc0006951.1_g000002
rosa_roxburghii	Rroxscaffold_3G00237380
rosa_rugosa	Rorug07G0205700
rosa_samantha	Rh7AG346900 Rh7BG336800 Rh7CG364200 Rh7DG344900
rosa_wichuraiana	Rw7G029380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	177
AccII	CGCG	1 cut(s)	42
AciI	CCGC	2 cut(s)	40, 664
AclWI	GGATC	1 cut(s)	642
AcsI	RAATTY	1 cut(s)	551
AfaI	GTAC	3 cut(s)	60, 184, 257
AfiI	CCNNNNNNNGG	1 cut(s)	177
AflII	CTTAAG	1 cut(s)	251
AgsI	TTSAA	1 cut(s)	551
AjnI	CCWGG	2 cut(s)	30, 61
AloI	GAACNNNNNNTCC	2 cut(s)	632, 664
AluBI	AGCT	6 cut(s)	17, 52, 382, 392, 577, 598
AluI	AGCT	6 cut(s)	17, 52, 382, 392, 577, 598
Alw26I	GTCTC	2 cut(s)	557, 617
AlwI	GGATC	1 cut(s)	642
AoxI	GGCC	2 cut(s)	64, 301
ApaI	GGGCCC	1 cut(s)	305
ApeKI	GCWGC	2 cut(s)	5, 598
ApoI	RAATTY	1 cut(s)	551
Asp700I	GAANNNNTTC	1 cut(s)	228
AspLEI	GCGC	1 cut(s)	44
AspS9I	GGNCC	3 cut(s)	301, 302, 539
AsuHPI	GGTGA	1 cut(s)	338
AvaII	GGWCC	1 cut(s)	539
BaeGI	GKGCMC	1 cut(s)	305
BanII	GRGCYC	1 cut(s)	305
BbvCI	CCTCAGC	1 cut(s)	426
BbvI	GCAGC	1 cut(s)	585
BccI	CCATC	1 cut(s)	434
BceAI	ACGGC	2 cut(s)	356, 432
BciT130I	CCWGG	2 cut(s)	32, 63
BcoDI	GTCTC	2 cut(s)	557, 617
BfrI	CTTAAG	1 cut(s)	251
BisI	GCNGC	2 cut(s)	6, 599
BlsI	GCNGC	2 cut(s)	7, 600
BmcAI	AGTACT	2 cut(s)	184, 257
Bme1390I	CCNGG	2 cut(s)	32, 63
Bme18I	GGWCC	1 cut(s)	539
BmgT120I	GGNCC	3 cut(s)	301, 302, 539
BmiI	GGNNCC	1 cut(s)	303
BmrFI	CCNGG	2 cut(s)	32, 63
BmsI	GCATC	3 cut(s)	231, 495, 514
BplI	GAGNNNNNCTC	2 cut(s)	138, 170
BpmI	CTGGAG	2 cut(s)	14, 199
Bpu10I	CCTNAGC	1 cut(s)	426
BpuEI	CTTGAG	1 cut(s)	623
BsaI	GGTCTC	1 cut(s)	617
BsaJI	CCNNGG	1 cut(s)	171
Bsc4I	CCNNNNNNNGG	1 cut(s)	177
Bse1I	ACTGG	2 cut(s)	105, 182
Bse3DI	GCAATG	2 cut(s)	71, 369
BseBI	CCWGG	2 cut(s)	32, 63
BseDI	CCNNGG	1 cut(s)	171
BseGI	GGATG	1 cut(s)	142
BseLI	CCNNNNNNNGG	1 cut(s)	177
BseMI	GCAATG	2 cut(s)	71, 369
BseMII	CTCAG	3 cut(s)	417, 627, 658
BseNI	ACTGG	2 cut(s)	105, 182
BseRI	GAGGAG	1 cut(s)	587
BseSI	GKGCMC	1 cut(s)	305
BseXI	GCAGC	1 cut(s)	585
BsgI	GTGCAG	1 cut(s)	387
Bsh1236I	CGCG	1 cut(s)	42
BshFI	GGCC	2 cut(s)	66, 303
BslFI	GGGAC	1 cut(s)	501
BslI	CCNNNNNNNGG	1 cut(s)	177
BsmAI	GTCTC	2 cut(s)	557, 617
BsmFI	GGGAC	1 cut(s)	501
BsnI	GGCC	2 cut(s)	66, 303
Bso31I	GGTCTC	1 cut(s)	617
Bsp120I	GGGCCC	1 cut(s)	301
Bsp1286I	GDGCHC	1 cut(s)	305
Bsp143I	GATC	4 cut(s)	9, 193, 613, 647
BspACI	CCGC	2 cut(s)	40, 664
BspANI	GGCC	2 cut(s)	66, 303
BspCNI	CTCAG	3 cut(s)	418, 628, 657
BspFNI	CGCG	1 cut(s)	42
BspHI	TCATGA	1 cut(s)	568
BspLI	GGNNCC	1 cut(s)	303
BspPI	GGATC	1 cut(s)	642
BspQI	GCTCTTC	1 cut(s)	354
BspTI	CTTAAG	1 cut(s)	251
BspTNI	GGTCTC	1 cut(s)	617
BsrDI	GCAATG	2 cut(s)	71, 369
BsrI	ACTGG	2 cut(s)	105, 182
BssECI	CCNNGG	1 cut(s)	171
BssMI	GATC	4 cut(s)	9, 193, 613, 647
BssT1I	CCWWGG	1 cut(s)	171
Bst2UI	CCWGG	2 cut(s)	32, 63
Bst4CI	ACNGT	2 cut(s)	187, 494
Bst6I	CTCTTC	1 cut(s)	354
BstAFI	CTTAAG	1 cut(s)	251
BstAPI	GCANNNNNTGC	1 cut(s)	375
BstC8I	GCNNGC	2 cut(s)	376, 380
BstDEI	CTNAG	4 cut(s)	13, 426, 636, 644
BstF5I	GGATG	1 cut(s)	142
BstFNI	CGCG	1 cut(s)	42
BstHHI	GCGC	1 cut(s)	44
BstKTI	GATC	4 cut(s)	12, 196, 616, 650
BstMAI	GTCTC	2 cut(s)	557, 617
BstMBI	GATC	4 cut(s)	9, 193, 613, 647
BstMWI	GCNNNNNNNGC	4 cut(s)	14, 41, 375, 663
BstNI	CCWGG	2 cut(s)	32, 63
BstNSI	RCATGY	3 cut(s)	335, 378, 516
BstSCI	CCNGG	2 cut(s)	30, 61
BstSLI	GKGCMC	1 cut(s)	305
BstUI	CGCG	1 cut(s)	42
BstV1I	GCAGC	1 cut(s)	585
BstX2I	RGATCY	1 cut(s)	647
BstYI	RGATCY	1 cut(s)	647
BsuRI	GGCC	2 cut(s)	66, 303
BtsCI	GGATG	1 cut(s)	142
BtsIMutI	CAGTG	1 cut(s)	219
Cac8I	GCNNGC	2 cut(s)	376, 380
CciI	TCATGA	1 cut(s)	568
CfoI	GCGC	1 cut(s)	44
Cfr13I	GGNCC	3 cut(s)	301, 302, 539
Csp6I	GTAC	3 cut(s)	59, 183, 256
CviAII	CATG	5 cut(s)	332, 375, 475, 513, 569
CviQI	GTAC	3 cut(s)	59, 183, 256
DdeI	CTNAG	4 cut(s)	13, 426, 636, 644
DpnI	GATC	4 cut(s)	11, 195, 615, 649
DpnII	GATC	4 cut(s)	9, 193, 613, 647
Eam1104I	CTCTTC	1 cut(s)	354
EarI	CTCTTC	1 cut(s)	354
Eco130I	CCWWGG	1 cut(s)	171
Eco24I	GRGCYC	1 cut(s)	305
Eco31I	GGTCTC	1 cut(s)	617
Eco32I	GATATC	1 cut(s)	347
Eco47I	GGWCC	1 cut(s)	539
EcoO109I	RGGNCCY	2 cut(s)	301, 302
EcoRI	GAATTC	1 cut(s)	551
EcoRII	CCWGG	2 cut(s)	30, 61
EcoRV	GATATC	1 cut(s)	347
EcoT14I	CCWWGG	1 cut(s)	171
EcoT38I	GRGCYC	1 cut(s)	305
ErhI	CCWWGG	1 cut(s)	171
FaeI	CATG	5 cut(s)	335, 378, 478, 516, 572
FaiI	YATR	9 cut(s)	93, 128, 203, 333, 376, 465, 476, 514, 570
FaqI	GGGAC	1 cut(s)	501
FatI	CATG	5 cut(s)	331, 374, 474, 512, 568
Fnu4HI	GCNGC	2 cut(s)	6, 599
FokI	GGATG	1 cut(s)	149
FriOI	GRGCYC	1 cut(s)	305
Fsp4HI	GCNGC	2 cut(s)	6, 599
GlaI	GCGC	1 cut(s)	43
GluI	GCNGC	2 cut(s)	6, 599
GsuI	CTGGAG	2 cut(s)	14, 199
HaeIII	GGCC	2 cut(s)	66, 303
HhaI	GCGC	1 cut(s)	44
Hin1II	CATG	5 cut(s)	335, 378, 478, 516, 572
Hin6I	GCGC	1 cut(s)	42
HinP1I	GCGC	1 cut(s)	42
HindIII	AAGCTT	1 cut(s)	380
HinfI	GANTC	3 cut(s)	310, 498, 565
HphI	GGTGA	1 cut(s)	338
Hpy166II	GTNNAC	2 cut(s)	110, 329
Hpy188I	TCNGA	4 cut(s)	21, 240, 647, 652
Hpy188III	TCNNGA	2 cut(s)	506, 569
Hpy8I	GTNNAC	2 cut(s)	110, 329
HpyAV	CCTTC	2 cut(s)	292, 530
HpyCH4III	ACNGT	2 cut(s)	187, 494
HpyCH4V	TGCA	7 cut(s)	76, 100, 369, 374, 378, 404, 486
HpyF10VI	GCNNNNNNNGC	4 cut(s)	14, 41, 375, 663
HpyF3I	CTNAG	4 cut(s)	13, 426, 636, 644
Hsp92II	CATG	5 cut(s)	335, 378, 478, 516, 572
HspAI	GCGC	1 cut(s)	42
Kzo9I	GATC	4 cut(s)	9, 193, 613, 647
LguI	GCTCTTC	1 cut(s)	354
LmnI	GCTCC	1 cut(s)	574
LpnPI	CCDG	8 cut(s)	17, 44, 48, 58, 75, 118, 163, 414
Lsp1109I	GCAGC	1 cut(s)	585
LweI	GCATC	3 cut(s)	231, 495, 514
MaeIII	GTNAC	1 cut(s)	274
MalI	GATC	4 cut(s)	11, 195, 615, 649
MboI	GATC	4 cut(s)	9, 193, 613, 647
MboII	GAAGA	3 cut(s)	75, 371, 449
MfeI	CAATTG	1 cut(s)	417
MflI	RGATCY	1 cut(s)	647
MhlI	GDGCHC	1 cut(s)	305
MluCI	AATT	3 cut(s)	283, 417, 551
MlyI	GAGTC	2 cut(s)	319, 559
MroXI	GAANNNNTTC	1 cut(s)	228
MseI	TTAA	1 cut(s)	252
MslI	CAYNNNNRTG	2 cut(s)	224, 473
MspA1I	CMGCKG	1 cut(s)	666
MspCI	CTTAAG	1 cut(s)	251
MspR9I	CCNGG	2 cut(s)	32, 63
MunI	CAATTG	1 cut(s)	417
MvaI	CCWGG	2 cut(s)	32, 63
MvnI	CGCG	1 cut(s)	42
MwoI	GCNNNNNNNGC	4 cut(s)	14, 41, 375, 663
NdeII	GATC	4 cut(s)	9, 193, 613, 647
NlaIII	CATG	5 cut(s)	335, 378, 478, 516, 572
NlaIV	GGNNCC	1 cut(s)	303
NspI	RCATGY	3 cut(s)	335, 378, 516
PaeI	GCATGC	1 cut(s)	378
PagI	TCATGA	1 cut(s)	568
PciSI	GCTCTTC	1 cut(s)	354
PdmI	GAANNNNTTC	1 cut(s)	228
PfeI	GAWTC	1 cut(s)	498
PflMI	CCANNNNNTGG	1 cut(s)	177
PkrI	GCNGC	2 cut(s)	7, 600
PleI	GAGTC	2 cut(s)	318, 559
PpsI	GAGTC	2 cut(s)	318, 559
Psp6I	CCWGG	2 cut(s)	30, 61
PspGI	CCWGG	2 cut(s)	30, 61
PspN4I	GGNNCC	1 cut(s)	303
PspOMI	GGGCCC	1 cut(s)	301
PspPI	GGNCC	3 cut(s)	301, 302, 539
PsrI	GAACNNNNNNTAC	2 cut(s)	321, 353
PsuI	RGATCY	1 cut(s)	647
RsaI	GTAC	3 cut(s)	60, 184, 257
RsaNI	GTAC	3 cut(s)	59, 183, 256
RseI	CAYNNNNRTG	2 cut(s)	224, 473
SapI	GCTCTTC	1 cut(s)	354
SaqAI	TTAA	1 cut(s)	252
SatI	GCNGC	2 cut(s)	6, 599
Sau3AI	GATC	4 cut(s)	9, 193, 613, 647
Sau96I	GGNCC	3 cut(s)	301, 302, 539
ScaI	AGTACT	2 cut(s)	184, 257
SchI	GAGTC	2 cut(s)	319, 559
ScrFI	CCNGG	2 cut(s)	32, 63
SduI	GDGCHC	1 cut(s)	305
SetI	ASST	9 cut(s)	19, 54, 238, 384, 394, 464, 579, 600, 645
SfaNI	GCATC	3 cut(s)	231, 495, 514
SinI	GGWCC	1 cut(s)	539
SmiMI	CAYNNNNRTG	2 cut(s)	224, 473
SmlI	CTYRAG	2 cut(s)	251, 602
SmoI	CTYRAG	2 cut(s)	251, 602
SphI	GCATGC	1 cut(s)	378
Sse9I	AATT	3 cut(s)	283, 417, 551
SsiI	CCGC	2 cut(s)	40, 664
StyD4I	CCNGG	2 cut(s)	30, 61
StyI	CCWWGG	1 cut(s)	171
TaaI	ACNGT	2 cut(s)	187, 494
TaqI	TCGA	3 cut(s)	196, 232, 534
TaqII	GACCGA	1 cut(s)	641
TasI	AATT	3 cut(s)	283, 417, 551
TatI	WGTACW	2 cut(s)	182, 255
TfiI	GAWTC	1 cut(s)	498
Tru1I	TTAA	1 cut(s)	252
Tru9I	TTAA	1 cut(s)	252
TscAI	CASTG	1 cut(s)	226
TseI	GCWGC	2 cut(s)	5, 598
TspRI	CASTG	1 cut(s)	226
Van91I	CCANNNNNTGG	1 cut(s)	177
Vha464I	CTTAAG	1 cut(s)	251
VpaK11BI	GGWCC	1 cut(s)	539
XapI	RAATTY	1 cut(s)	551
XceI	RCATGY	3 cut(s)	335, 378, 516
XmnI	GAANNNNTTC	1 cut(s)	228
ZrmI	AGTACT	2 cut(s)	184, 257

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.