AT1G18270

Ketose-bisphosphate aldolase class-II family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006366
Physical Location & Seq
Reverse (-)
6283370 .. 6293993
10624 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18270.2

Sequence Viewer

Length: 4122 bp
ATGAGTGGCGTGGTTGGGTTTGTTGGTCTTGATTCTTTCAGCTTCGAATTAGCTTCTTCGCTTCTTCGTTCTGGGTTTAAGGTTCAAGCATTCGAGATAAGTACAGAATTAGTGGAGAAGTTCATTGAATTGGGAGGACACAAATGTGACAGCCCTGCAGATGTTGGGAAAGCTGCAGCAGCCGTTGTGGTTGTACTATCCCACCCTGACCAAATTCAAGATGTCATTTTTGGTGATGAGGGTGTTATGAAAGGTCTACAGAAGGACGCTGTATTGCTTTTGTCTTCGACAATATCGACTTTGCAACTCCAGAAACTAGAGAAACAACTTACAGAGAAAAGAGAGCAGATTTTTGTAGTTGATGCCTATGTTTTAAAAGGGATGTCTGAGCTTCTTGATGGAAAACTAATGATAATAGCATCTGGAAGGTCAGATTCGATCACAAGAGCACAACCCTATCTTACTGCAATGTGTCAAAATCTGTACACCTTTGAGGGAGAAATTGGTGCTGGAAGTAAAGTTAAAATGGTGAATGAGTTGCTTGAGGGCATACATCTTGTTGCTGCTGTGGAGGCTATATCTTTAGGTTCTCAGGCGGGTGTGCATCCATGGATACTTTATGACATAATTTCCAATGCTGCTGGAAATTCATGGATTTACAAGAATCATATTCCGTTGTTGCTAAAAGATGACATAGAGGGTCGCTTCCTCGATGTTCTCTCTCAGAATCTGGCCATTGTTGAAGATAAAGCCAAATCGCTCCCATTTCCTGTCCCACTTTTGGCAGTTGCACGCCAACAACTAATCTCTGGAATATCACAGATGCAGGGAGATGATACAGCTACTTCATTGGCTAAGATCTCGGAAAAAGTACTTGGTGTTGGCATATTAGAAGCAGCCAATAGAGAACTTTATAAGCCAGAGGATTTGGCCAAAGAAATTACTACTCAAGCAAAGCCTGTGAATAGAATTGGTTTTATTGGTCTTGGAGCAATGGGTTTTGGCATGGCAGCACACCTGTTGAAATCAAATTTTTCTGTCTGTGGTTATGACGTATACAAGCCAACACTTGTCAGATTTGAGAATGCCGGGGGATTGGCGGCAAATTCTCCAGCTGAAGTGACGAAAGATGTAGATGTTCTTGTAATTATGGTAACAAACGAGGTTCAGGCTGAGGATGTCTTGTATGGACATCTTGGAGCGGTTGAAGCTATACCATCTGGAGCAACTGTTGTACTTGCGTCAACAGTATCTCCCGCGTTTGTTAGCCAACTTGAAAGGCGTCTAGAAAATGAGGGAAAAGACCTAAAGCTAGTTGATGCTCCTGTGTCTGGTGGTGTCAAGAGGGCTGCCATGGGGGAGCTTACGATAATGGCTTCAGGTACCGATGAAGCACTTAAGTCTGCGGGACTTGTGTTATCAGCATTAAGTGAGAAGCTCTACGTGATTAAAGGAGGTTGTGGGGCAGGAAGTGGTGTAAAAATGGTCAATCAATTGCTTGCGGGAGTTCATATCGCATCAGCTGCCGAGGCAATGGCATTTGGAGCTCGGTTAGGTCTGAATACTCGAAAGCTATTCAATGTTATTTCCAACAGCGGTGGAACATCTTGGATGTTTGAAAATCGTGTTCCGCACATGCTGGATAATGATTACACACCATACTCTGCTCTTGATATTTTTGTCAAGGATTTGGGAATTGTTACCCGAGAAGGTTCATCCCGTAAAGTTCCACTCCATATATCAACAGTGGCACACCAGCTATTCTTAGCCGGCTCTGCTGCGGGCTGGGGACGGATAGATGATGCTGGAGTGGTGAAGGTTTATGAAACCCTTGCTGGAATTAAAGTCGAAGGGAGACTTCCTGTTCTGAAGAAACAAGACCTACTGAAATCTCTTCCAGCTGAATGGCCTTCAGACCCTACAACTGACATACATAGACTAAATATGGGAAACTCGAAGACATTGGTTGTTCTTGATGATGATCCAACTGGCACACAAACTGTTCATGACGTAGAGGTTTTAACTGAATGGAGTGTTGAATCTATTTCTGAGCAGTTTAGAAAAAAGCCTGCATGCTTCTTCATTTTGACAAATTCAAGGTCCCTGAGCCCTGAGAAGGCTAGTGAATTGATCAAAGACATCTGTTCCAACCTATGTGCTGCATCAAAAGAAGTTGGCAATGCTGACTACACGATTGTTCTGAGGGGTGATTCAACATTACGTGGTCATTTTCCTCAGGAAGCAGATGCTGCTGTCTCAATACTTGGGGAAATGGATGCATGGATTATTTGCCCCTTCTTCCTTCAAGGGGGACGTTATACTATTGATGATGTGCATTATGTTGCGGATTCTGATAGGCTCGTTCCTGCAGGAGAGACAGAGTTTGCCAAAGATGCATCCTTTGGATATAAATCCTCAAATCTCCGTGAGTGGGTTGAGGAGAAAACAGCTGGCGTCATTCCGGCTAACAGCGTTCAGTCGATCTCAATTCAGCTTTTAAGAAAAGGTGGCCCTGATGCAGTGTGTGAGTTTCTGTGCAGTTTAAAGAAGGGGTCTACATGTATTGTCAACGCCGCTAGTGAAAGAGACATGGCTGTATTTGCAGCCGGGATGATCCAGGCAGAACTGAAAGGGAGATCCTTTTTATGCCGCACTGCTGCTAGCTTTGTGTCTGCTCTAATAGGGATTATTCCAAAAGATCCTGTGTTGCCAAAAGATTTCGAATCTAACAAAGAAAGCAGTGGTGCACTAATAGTTGTAGGCTCATATGTCCCGAAAACAACAAAGCAGGTTGAAGAACTTCAATCACAACATAACCAGAATTTAAGGAGCATCGAGATCTCTGTAGAAAAAGTTGCTCTCAAGTCATCTGAAGTGAGAGATGAAGAAATCAGAAGAGCAGTTGAGATGGCCGATGCTTTCCTTCGGGCTGGCAGGGAGACTCTAATTATGAGCAGTCGTGAACTCATCACGGGAAAAACATCTTCGGAAAGTCTTGATATTAACAGCAAAGTGAGCTCCGCTCTGGTTGAAGTAGTGAGCCAGATATCGACCAGACCTCGTTATATTCTTGCTAAGGGTGGAATTACGTCATCAGACACAGCAACGAAAGCCCTGAAAGCACGACGTGCACTTGTGATTGGTCAAGCCCTTGCTGGTGTTCCAGTATGGAAGTTAGGTCCAGAAAGTAGACATCCAGGGGTCCCCTACATTGTTTTCCCTGGTAATGTTGGTAACAGCACAGCGCTTGCAGAAGTTGTGAAGTCTTGGTCAGTGGTAGCAGGACGGTCGACAAAAGAGCTGCTTCTGAATGCAGAAAAAGGTGGATATGCAGTTGGAGCATTTAACGTGTATAACCTGGAAGGAATAGAAGCTGTTGTAGCGGCCGCAGAGGAAGAAAATAGTCCTGCTATATTGCAGGTCCATCCAGGTGCCTTTAAACAAGGAGGCATTCCTTTGGTTTCATGTTGCATCTCTGCAGCTGAACAAGCCAGGGTTCCAATATCCGTACACTTTGATCATGGAACTACAAAGCATGAGCTATTAGAAGCTCTTGAGCTAGGACTAGATTCAGTGATGGTAGATGGTTCTCATCTTTCCTTCACAGAAAATTTATCCTACACGAAATCCATAACAGAGTTGGCCCGTTCCAAAAACATTATGGTGGAAGCTGAACTAGGCCGACTCTCAGGAACAGAGGATGGCTTGACTGTTGAAGACTATGAAGCAAAGCTCACCAACGTTAACCAGGCTCAGGAATTTATGGAAACTGGTATAGATGCTTTGGCTGTGTGTATAGGAAATGTCCATGGAAAGTACCCCAAGTCCGGTCCAAACCTCAAGCTTGATTTACTTAAGGAACTGCACGCCTTAAGCTCGAAGAAAGGCGTTTTTCTTGTTCTTCACGGAGCTTCTGGTTTGTCTGAGAACCTTATCAAGGAATGCATTGAGAATGGAGTGAGGAAGTTCAACGTGAACACAGAAGTAAGAACGGCTTACATGGAAGCTCTTAGCTCTGGGAAGAAGACCGACATTGTTGATGTCATGTCCGCAACAAAAGCAGCCATGAAAGCTGTGATCGCAGATAAGATCCGTCTCTTTGGCTCTGCCGGAAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1373

Amino Acids

147.04

Weight (kDa)

5.6

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 5 - 163 3e-22 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 170 - 287 3.6e-20 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 324 - 483 2.4e-35 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 489 - 609 3.3e-30 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 656 - 894 1.7e-58 Sugar-binding N-terminal domain
NBD_C PF17042 918 - 1084 1.8e-36 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1098 - 1373 7.9e-91 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
Acc36I ACCTGC 2 cut(s) 2779, 3409
Acc65I GGTACC 1 cut(s) 1382
AccB1I GGYRCC 2 cut(s) 1382, 3431
AccBSI CCGCTC 2 cut(s) 1202, 3023
AccI GTMKAC 5 cut(s) 256, 1056, 2555, 3190, 3290
AccII CGCG 1 cut(s) 1259
AclI AACGTT 1 cut(s) 3741
AclWI GGATC 5 cut(s) 1976, 2608, 2631, 2693, 4084
AcoI YGGCCR 4 cut(s) 732, 930, 2910, 3384
AcsI RAATTY 8 cut(s) 213, 646, 1030, 1105, 2092, 2821, 3610, 3758
AcuI CTGAAG 5 cut(s) 1137, 1362, 1889, 1896, 2892
AcyI GRCGYC 2 cut(s) 1282, 2454
AdeI CACNNNGTG 1 cut(s) 3128
AfaI GTAC 8 cut(s) 103, 195, 485, 873, 1236, 1384, 3510, 3818
AfeI AGCGCT 1 cut(s) 3246
AfiI CCNNNNNNNGG 8 cut(s) 781, 1331, 2116, 2308, 2431, 3754, 3827, 3937
AflII CTTAAG 3 cut(s) 1397, 3854, 3871
AflIII ACRYGT 2 cut(s) 2558, 3348
AjiI CACGTC 1 cut(s) 3128
AjnI CCWGG 7 cut(s) 2616, 3196, 3220, 3357, 3427, 3491, 3747
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
AleI CACNNNNGTG 1 cut(s) 144
Alw21I GWGCWC 5 cut(s) 451, 1549, 2749, 3020, 3133
Alw26I GTCTC 6 cut(s) 1849, 2260, 2369, 2580, 2933, 4100
Alw44I GTGCAC 2 cut(s) 2745, 3129
AlwI GGATC 5 cut(s) 1976, 2608, 2631, 2693, 4084
AlwNI CAGNNNCTG 2 cut(s) 730, 2249
Ama87I CYCGRG 1 cut(s) 1704
Aor51HI AGCGCT 1 cut(s) 3246
AoxI GGCC 8 cut(s) 732, 930, 1907, 2509, 2910, 3384, 3642, 3679
ApaLI GTGCAC 2 cut(s) 2745, 3129
ApoI RAATTY 8 cut(s) 213, 646, 1030, 1105, 2092, 2821, 3610, 3758
ArsI GACNNNNNNTTYG 2 cut(s) 2084, 2116
Asp700I GAANNNNTTC 1 cut(s) 2799
Asp718I GGTACC 1 cut(s) 1382
AspLEI GCGC 1 cut(s) 3247
AspS9I GGNCC 7 cut(s) 2100, 2510, 3179, 3202, 3421, 3643, 3830
AsuC2I CCSGG 2 cut(s) 1090, 2608
AsuHPI GGTGA 5 cut(s) 245, 541, 1825, 2219, 3727
AsuII TTCGAA 2 cut(s) 45, 2721
AsuNHI GCTAGC 1 cut(s) 2660
AvaI CYCGRG 1 cut(s) 1704
AvaII GGWCC 5 cut(s) 2100, 3179, 3202, 3421, 3830
AxyI CCTNAGG 1 cut(s) 2235
BaeGI GKGCMC 2 cut(s) 2749, 3133
BalI TGGCCA 2 cut(s) 734, 932
BanI GGYRCC 2 cut(s) 1382, 3431
BanII GRGCYC 3 cut(s) 1549, 2111, 3020
BbsI GAAGAC 4 cut(s) 276, 1964, 3723, 4031
Bbv12I GWGCWC 5 cut(s) 451, 1549, 2749, 3020, 3133
BbvCI CCTCAGC 1 cut(s) 1173
BccI CCATC 7 cut(s) 392, 1225, 2902, 3432, 3571, 3578, 3695
BceAI ACGGC 2 cut(s) 167, 4008
BcgI CGANNNNNNTGC 2 cut(s) 3270, 3304
BciT130I CCWGG 7 cut(s) 2618, 3198, 3222, 3359, 3429, 3493, 3749
BciVI GTATCC 1 cut(s) 606
BclI TGATCA 2 cut(s) 2130, 3517
BcnI CCSGG 2 cut(s) 1090, 2608
BcoDI GTCTC 6 cut(s) 1849, 2260, 2369, 2580, 2933, 4100
BfaI CTAG 9 cut(s) 317, 1286, 1313, 2121, 2577, 2661, 3560, 3566, 3677
BfmI CTRYAG 6 cut(s) 156, 174, 257, 2367, 2844, 3477
BfoI RGCGCY 1 cut(s) 3248
BfrI CTTAAG 3 cut(s) 1397, 3854, 3871
BfuAI ACCTGC 2 cut(s) 2779, 3409
BfuI GTATCC 1 cut(s) 606
BglII AGATCT 2 cut(s) 858, 2838
BmcAI AGTACT 1 cut(s) 873
Bme1390I CCNGG 9 cut(s) 1090, 2608, 2618, 3198, 3222, 3359, 3429, 3493, 3749
Bme18I GGWCC 5 cut(s) 2100, 3179, 3202, 3421, 3830
BmeT110I CYCGRG 1 cut(s) 1704
BmgBI CACGTC 1 cut(s) 3128
BmgT120I GGNCC 7 cut(s) 2100, 2510, 3179, 3202, 3421, 3643, 3830
BmiI GGNNCC 6 cut(s) 1384, 2102, 3203, 3204, 3433, 3498
BmrFI CCNGG 9 cut(s) 1090, 2608, 2618, 3198, 3222, 3359, 3429, 3493, 3749
BmtI GCTAGC 1 cut(s) 2664
BpiI GAAGAC 4 cut(s) 276, 1964, 3723, 4031
BplI GAGNNNNNCTC 2 cut(s) 3007, 3039
BpmI CTGGAG 4 cut(s) 293, 1095, 1242, 1827
Bpu10I CCTNAGC 4 cut(s) 1173, 2105, 3075, 3753
Bpu14I TTCGAA 2 cut(s) 45, 2721
BpuEI CTTGAG 5 cut(s) 563, 933, 2846, 3575, 3824
BpuMI CCSGG 2 cut(s) 1090, 2608
BsaAI YACGTR 2 cut(s) 1444, 2222
BsaBI GATNNNNATC 2 cut(s) 1980, 2410
BsaHI GRCGYC 2 cut(s) 1282, 2454
BsaJI CCNNGG 8 cut(s) 608, 1089, 1353, 1527, 3197, 3220, 3492, 3808
BsaWI WCCGGW 1 cut(s) 3827
BsaXI ACNNNNNCTCC 4 cut(s) 1215, 1237, 1245, 1267
Bsc4I CCNNNNNNNGG 8 cut(s) 781, 1331, 2116, 2308, 2431, 3754, 3827, 3937
Bse118I RCCGGY 1 cut(s) 1769
Bse1I ACTGG 3 cut(s) 1993, 3164, 3775
Bse21I CCTNAGG 1 cut(s) 2235
Bse3DI GCAATG 4 cut(s) 474, 999, 1539, 2185
Bse8I GATNNNNATC 2 cut(s) 1980, 2410
BseBI CCWGG 7 cut(s) 2618, 3198, 3222, 3359, 3429, 3493, 3749
BseDI CCNNGG 8 cut(s) 608, 1089, 1353, 1527, 3197, 3220, 3492, 3808
BseJI GATNNNNATC 2 cut(s) 1980, 2410
BseLI CCNNNNNNNGG 8 cut(s) 781, 1331, 2116, 2308, 2431, 3754, 3827, 3937
BseMI GCAATG 4 cut(s) 474, 999, 1539, 2185
BseNI ACTGG 3 cut(s) 1993, 3164, 3775
BseRI GAGGAG 1 cut(s) 2453
BseSI GKGCMC 2 cut(s) 2749, 3133
BseX3I CGGCCG 1 cut(s) 3384
BseYI CCCAGC 1 cut(s) 1785
BsgI GTGCAG 2 cut(s) 2557, 3848
Bsh1236I CGCG 1 cut(s) 1259
Bsh1285I CGRYCG 2 cut(s) 3290, 3387
BshFI GGCC 8 cut(s) 734, 932, 1909, 2511, 2912, 3386, 3644, 3681
BshNI GGYRCC 2 cut(s) 1382, 3431
BsiEI CGRYCG 2 cut(s) 3290, 3387
BsiHKAI GWGCWC 5 cut(s) 451, 1549, 2749, 3020, 3133
BsiHKCI CYCGRG 1 cut(s) 1704
BsiSI CCGG 6 cut(s) 1089, 1770, 2462, 2607, 3828, 4110
BslFI GGGAC 7 cut(s) 758, 1422, 1803, 2086, 2325, 2756, 3188
BslI CCNNNNNNNGG 8 cut(s) 781, 1331, 2116, 2308, 2431, 3754, 3827, 3937
BsmAI GTCTC 6 cut(s) 1849, 2260, 2369, 2580, 2933, 4100
BsmBI CGTCTC 1 cut(s) 4100
BsmFI GGGAC 7 cut(s) 758, 1422, 1803, 2086, 2325, 2756, 3188
BsmI GAATGC 5 cut(s) 89, 1090, 3316, 3450, 3947
BsnI GGCC 8 cut(s) 734, 932, 1909, 2511, 2912, 3386, 3644, 3681
BsoBI CYCGRG 1 cut(s) 1704
Bsp119I TTCGAA 2 cut(s) 45, 2721
Bsp1286I GDGCHC 6 cut(s) 451, 1549, 2111, 2749, 3020, 3133
Bsp1407I TGTACA 1 cut(s) 483
Bsp19I CCATGG 3 cut(s) 608, 1353, 3808
BspANI GGCC 8 cut(s) 734, 932, 1909, 2511, 2912, 3386, 3644, 3681
BspFNI CGCG 1 cut(s) 1259
BspHI TCATGA 1 cut(s) 2005
BspLI GGNNCC 6 cut(s) 1384, 2102, 3203, 3204, 3433, 3498
BspMAI CTGCAG 4 cut(s) 160, 178, 2371, 3481
BspMI ACCTGC 2 cut(s) 2779, 3409
BspOI GCTAGC 1 cut(s) 2664
BspPI GGATC 5 cut(s) 1976, 2608, 2631, 2693, 4084
BspQI GCTCTTC 1 cut(s) 2890
BspT104I TTCGAA 2 cut(s) 45, 2721
BspT107I GGYRCC 2 cut(s) 1382, 3431
BspTI CTTAAG 3 cut(s) 1397, 3854, 3871
BsrBI CCGCTC 2 cut(s) 1202, 3023
BsrDI GCAATG 4 cut(s) 474, 999, 1539, 2185
BsrFI RCCGGY 1 cut(s) 1769
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 3 cut(s) 1993, 3164, 3775
BssAI RCCGGY 1 cut(s) 1769
BssECI CCNNGG 8 cut(s) 608, 1089, 1353, 1527, 3197, 3220, 3492, 3808
BssNAI GTATAC 1 cut(s) 1057
BssNI GRCGYC 2 cut(s) 1282, 2454
BssT1I CCWWGG 3 cut(s) 608, 1353, 3808
Bst1107I GTATAC 1 cut(s) 1057
Bst2UI CCWGG 7 cut(s) 2618, 3198, 3222, 3359, 3429, 3493, 3749
Bst4CI ACNGT 6 cut(s) 1231, 1249, 1747, 2002, 3288, 3712
Bst6I CTCTTC 2 cut(s) 1899, 2890
BstACI GRCGYC 2 cut(s) 1282, 2454
BstAFI CTTAAG 3 cut(s) 1397, 3854, 3871
BstAPI GCANNNNNTGC 3 cut(s) 1523, 2249, 3128
BstAUI TGTACA 1 cut(s) 483
BstBAI YACGTR 2 cut(s) 1444, 2222
BstBI TTCGAA 2 cut(s) 45, 2721
BstDSI CCRYGG 3 cut(s) 608, 1353, 3808
BstENI CCTNNNNNAGG 1 cut(s) 3935
BstFNI CGCG 1 cut(s) 1259
BstH2I RGCGCY 1 cut(s) 3248
BstHHI GCGC 1 cut(s) 3247
BstMAI GTCTC 6 cut(s) 1849, 2260, 2369, 2580, 2933, 4100
BstMCI CGRYCG 2 cut(s) 3290, 3387
BstNI CCWGG 7 cut(s) 2618, 3198, 3222, 3359, 3429, 3493, 3749
BstNSI RCATGY 3 cut(s) 1639, 2076, 2562
BstSCI CCNGG 9 cut(s) 1088, 2606, 2616, 3196, 3220, 3357, 3427, 3491, 3747
BstSFI CTRYAG 6 cut(s) 156, 174, 257, 2367, 2844, 3477
BstSLI GKGCMC 2 cut(s) 2749, 3133
BstUI CGCG 1 cut(s) 1259
BstV2I GAAGAC 4 cut(s) 276, 1964, 3723, 4031
BstX2I RGATCY 5 cut(s) 858, 2636, 2698, 2838, 4089
BstXI CCANNNNNNTGG 1 cut(s) 1905
BstYI RGATCY 5 cut(s) 858, 2636, 2698, 2838, 4089
BstZ17I GTATAC 1 cut(s) 1057
BstZI CGGCCG 1 cut(s) 3384
Bsu36I CCTNAGG 1 cut(s) 2235
BsuI GTATCC 1 cut(s) 606
BsuRI GGCC 8 cut(s) 734, 932, 1909, 2511, 2912, 3386, 3644, 3681
BtgI CCRYGG 3 cut(s) 608, 1353, 3808
BtrI CACGTC 1 cut(s) 3128
BtsI GCAGTG 3 cut(s) 2526, 2652, 2746
BtsIMutI CAGTG 6 cut(s) 1752, 2526, 2652, 2746, 3279, 3579
BveI ACCTGC 2 cut(s) 2779, 3409
CaiI CAGNNNCTG 2 cut(s) 730, 2249
CciI TCATGA 1 cut(s) 2005
CciNI GCGGCCGC 1 cut(s) 3384
CfoI GCGC 1 cut(s) 3247
Cfr10I RCCGGY 1 cut(s) 1769
Cfr13I GGNCC 7 cut(s) 2100, 2510, 3179, 3202, 3421, 3643, 3830
CseI GACGC 4 cut(s) 275, 1230, 1271, 2443
Csp6I GTAC 8 cut(s) 102, 194, 484, 872, 1235, 1383, 3509, 3817
CspCI CAANNNNNGTGG 2 cut(s) 1579, 1614
CviQI GTAC 8 cut(s) 102, 194, 484, 872, 1235, 1383, 3509, 3817
DraI TTTAAA 3 cut(s) 375, 2544, 3439
DraIII CACNNNGTG 1 cut(s) 3128
EaeI YGGCCR 4 cut(s) 732, 930, 2910, 3384
EagI CGGCCG 1 cut(s) 3384
Eam1104I CTCTTC 2 cut(s) 1899, 2890
EarI CTCTTC 2 cut(s) 1899, 2890
Ecl136II GAGCTC 2 cut(s) 1547, 3018
EclXI CGGCCG 1 cut(s) 3384
Eco130I CCWWGG 3 cut(s) 608, 1353, 3808
Eco24I GRGCYC 3 cut(s) 1549, 2111, 3020
Eco32I GATATC 1 cut(s) 3048
Eco47I GGWCC 5 cut(s) 2100, 3179, 3202, 3421, 3830
Eco47III AGCGCT 1 cut(s) 3246
Eco52I CGGCCG 1 cut(s) 3384
Eco53kI GAGCTC 2 cut(s) 1547, 3018
Eco57I CTGAAG 5 cut(s) 1137, 1362, 1889, 1896, 2892
Eco81I CCTNAGG 1 cut(s) 2235
Eco88I CYCGRG 1 cut(s) 1704
EcoICRI GAGCTC 2 cut(s) 1547, 3018
EcoNI CCTNNNNNAGG 1 cut(s) 3935
EcoO109I RGGNCCY 2 cut(s) 2100, 3202
EcoRII CCWGG 7 cut(s) 2616, 3196, 3220, 3357, 3427, 3491, 3747
EcoRV GATATC 1 cut(s) 3048
EcoT14I CCWWGG 3 cut(s) 608, 1353, 3808
EcoT22I ATGCAT 3 cut(s) 2281, 2398, 3947
EcoT38I GRGCYC 3 cut(s) 1549, 2111, 3020
ErhI CCWWGG 3 cut(s) 608, 1353, 3808
Esp3I CGTCTC 1 cut(s) 4100
FalI AAGNNNNNCTT 6 cut(s) 1842, 1874, 3288, 3320, 3979, 4011
FaqI GGGAC 7 cut(s) 758, 1422, 1803, 2086, 2325, 2756, 3188
FauI CCCGC 5 cut(s) 589, 1264, 1399, 1495, 1774
FauNDI CATATG 1 cut(s) 2767
FbaI TGATCA 2 cut(s) 2130, 3517
FblI GTMKAC 5 cut(s) 256, 1056, 2555, 3190, 3290
FriOI GRGCYC 3 cut(s) 1549, 2111, 3020
FspBI CTAG 9 cut(s) 317, 1286, 1313, 2121, 2577, 2661, 3560, 3566, 3677
GlaI GCGC 1 cut(s) 3246
GsaI CCCAGC 1 cut(s) 1789
GsuI CTGGAG 4 cut(s) 293, 1095, 1242, 1827
HaeII RGCGCY 1 cut(s) 3248
HaeIII GGCC 8 cut(s) 734, 932, 1909, 2511, 2912, 3386, 3644, 3681
HapII CCGG 6 cut(s) 1089, 1770, 2462, 2607, 3828, 4110
HgaI GACGC 4 cut(s) 275, 1230, 1271, 2443
HhaI GCGC 1 cut(s) 3247
Hin1I GRCGYC 2 cut(s) 1282, 2454
Hin6I GCGC 1 cut(s) 3245
HinP1I GCGC 1 cut(s) 3245
HincII GTYRAC 4 cut(s) 1245, 2569, 3291, 3745
HindII GTYRAC 4 cut(s) 1245, 2569, 3291, 3745
HindIII AAGCTT 2 cut(s) 3842, 4116
HpaI GTTAAC 1 cut(s) 3745
HpaII CCGG 6 cut(s) 1089, 1770, 2462, 2607, 3828, 4110
HphI GGTGA 5 cut(s) 245, 541, 1825, 2219, 3727
Hpy99I CGWCG 1 cut(s) 3129
HpyCH4III ACNGT 6 cut(s) 1231, 1249, 1747, 2002, 3288, 3712
Hsp92I GRCGYC 2 cut(s) 1282, 2454
HspAI GCGC 1 cut(s) 3245
KflI GGGWCCC 1 cut(s) 3202
KpnI GGTACC 1 cut(s) 1386
KroI GCCGGC 1 cut(s) 1769
KroNI GCCGGC 1 cut(s) 1771
Ksp22I TGATCA 2 cut(s) 2130, 3517
KspAI GTTAAC 1 cut(s) 3745
LguI GCTCTTC 1 cut(s) 2890
MaeI CTAG 9 cut(s) 317, 1286, 1313, 2121, 2577, 2661, 3560, 3566, 3677
MaeIII GTNAC 5 cut(s) 146, 1120, 1153, 1699, 3233
MbiI CCGCTC 2 cut(s) 1202, 3023
MfeI CAATTG 1 cut(s) 1493
MflI RGATCY 5 cut(s) 858, 2636, 2698, 2838, 4089
MhlI GDGCHC 6 cut(s) 451, 1549, 2111, 2749, 3020, 3133
MlsI TGGCCA 2 cut(s) 734, 932
MluNI TGGCCA 2 cut(s) 734, 932
MlyI GAGTC 2 cut(s) 2935, 3678
MmeI TCCRAC 4 cut(s) 1614, 2009, 2172, 3316
Mox20I TGGCCA 2 cut(s) 734, 932
Mph1103I ATGCAT 3 cut(s) 2281, 2398, 3947
MroNI GCCGGC 1 cut(s) 1769
MroXI GAANNNNTTC 1 cut(s) 2799
MscI TGGCCA 2 cut(s) 734, 932
MslI CAYNNNNRTG 3 cut(s) 144, 3429, 3662
Msp20I TGGCCA 2 cut(s) 734, 932
MspA1I CMGCKG 6 cut(s) 1115, 1523, 1596, 1901, 2450, 3482
MspCI CTTAAG 3 cut(s) 1397, 3854, 3871
MspI CCGG 6 cut(s) 1089, 1770, 2462, 2607, 3828, 4110
MspR9I CCNGG 9 cut(s) 1090, 2608, 2618, 3198, 3222, 3359, 3429, 3493, 3749
MunI CAATTG 1 cut(s) 1493
Mva1269I GAATGC 5 cut(s) 89, 1090, 3316, 3450, 3947
MvaI CCWGG 7 cut(s) 2618, 3198, 3222, 3359, 3429, 3493, 3749
MvnI CGCG 1 cut(s) 1259
NaeI GCCGGC 1 cut(s) 1771
NciI CCSGG 2 cut(s) 1090, 2608
NcoI CCATGG 3 cut(s) 608, 1353, 3808
NdeI CATATG 1 cut(s) 2767
NgoMIV GCCGGC 1 cut(s) 1769
NheI GCTAGC 1 cut(s) 2660
NlaIV GGNNCC 6 cut(s) 1384, 2102, 3203, 3204, 3433, 3498
NmeAIII GCCGAG 1 cut(s) 1552
NmuCI GTSAC 2 cut(s) 146, 1120
NotI GCGGCCGC 1 cut(s) 3384
NsiI ATGCAT 3 cut(s) 2281, 2398, 3947
NspI RCATGY 3 cut(s) 1639, 2076, 2562
NspV TTCGAA 2 cut(s) 45, 2721
OliI CACNNNNGTG 1 cut(s) 144
PaeI GCATGC 1 cut(s) 2076
PagI TCATGA 1 cut(s) 2005
PciI ACATGT 1 cut(s) 2558
PciSI GCTCTTC 1 cut(s) 2890
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PctI GAATGC 5 cut(s) 89, 1090, 3316, 3450, 3947
PdiI GCCGGC 1 cut(s) 1771
PdmI GAANNNNTTC 1 cut(s) 2799
PfeI GAWTC 9 cut(s) 32, 434, 664, 727, 2039, 2210, 2348, 2723, 3569
PleI GAGTC 2 cut(s) 2935, 3678
PpsI GAGTC 2 cut(s) 2935, 3678
Ppu21I YACGTR 2 cut(s) 1444, 2222
PpuMI RGGWCCY 2 cut(s) 2100, 3202
PscI ACATGT 1 cut(s) 2558
PsiI TTATAA 1 cut(s) 915
Psp124BI GAGCTC 2 cut(s) 1549, 3020
Psp1406I AACGTT 1 cut(s) 3741
Psp5II RGGWCCY 2 cut(s) 2100, 3202
Psp6I CCWGG 7 cut(s) 2616, 3196, 3220, 3357, 3427, 3491, 3747
PspFI CCCAGC 1 cut(s) 1785
PspGI CCWGG 7 cut(s) 2616, 3196, 3220, 3357, 3427, 3491, 3747
PspN4I GGNNCC 6 cut(s) 1384, 2102, 3203, 3204, 3433, 3498
PspPI GGNCC 7 cut(s) 2100, 2510, 3179, 3202, 3421, 3643, 3830
PspPPI RGGWCCY 2 cut(s) 2100, 3202
PstI CTGCAG 4 cut(s) 160, 178, 2371, 3481
PstNI CAGNNNCTG 2 cut(s) 730, 2249
PsuI RGATCY 5 cut(s) 858, 2636, 2698, 2838, 4089
PvuII CAGCTG 5 cut(s) 1115, 1523, 1901, 2450, 3482
RsaI GTAC 8 cut(s) 103, 195, 485, 873, 1236, 1384, 3510, 3818
RsaNI GTAC 8 cut(s) 102, 194, 484, 872, 1235, 1383, 3509, 3817
RseI CAYNNNNRTG 3 cut(s) 144, 3429, 3662
SacI GAGCTC 2 cut(s) 1549, 3020
SalI GTCGAC 1 cut(s) 3289
SapI GCTCTTC 1 cut(s) 2890
Sau96I GGNCC 7 cut(s) 2100, 2510, 3179, 3202, 3421, 3643, 3830
SbfI CCTGCAGG 1 cut(s) 2371
ScaI AGTACT 1 cut(s) 873
SchI GAGTC 2 cut(s) 2935, 3678
ScrFI CCNGG 9 cut(s) 1090, 2608, 2618, 3198, 3222, 3359, 3429, 3493, 3749
SdaI CCTGCAGG 1 cut(s) 2371
SduI GDGCHC 6 cut(s) 451, 1549, 2111, 2749, 3020, 3133
SfcI CTRYAG 6 cut(s) 156, 174, 257, 2367, 2844, 3477
SfuI TTCGAA 2 cut(s) 45, 2721
SinI GGWCC 5 cut(s) 2100, 3179, 3202, 3421, 3830
SmiMI CAYNNNNRTG 3 cut(s) 144, 3429, 3662
SmlI CTYRAG 8 cut(s) 542, 948, 1397, 2861, 3554, 3839, 3854, 3871
SmoI CTYRAG 8 cut(s) 542, 948, 1397, 2861, 3554, 3839, 3854, 3871
SphI GCATGC 1 cut(s) 2076
Sse8387I CCTGCAGG 1 cut(s) 2371
SspMI CTAG 9 cut(s) 317, 1286, 1313, 2121, 2577, 2661, 3560, 3566, 3677
SstI GAGCTC 2 cut(s) 1549, 3020
StyD4I CCNGG 9 cut(s) 1088, 2606, 2616, 3196, 3220, 3357, 3427, 3491, 3747
StyI CCWWGG 3 cut(s) 608, 1353, 3808
TaaI ACNGT 6 cut(s) 1231, 1249, 1747, 2002, 3288, 3712
TaqII GACCGA 1 cut(s) 4043
TatI WGTACW 5 cut(s) 101, 193, 483, 871, 1234
TauI GCSGC 5 cut(s) 1103, 2576, 2652, 3386, 3389
TfiI GAWTC 9 cut(s) 32, 434, 664, 727, 2039, 2210, 2348, 2723, 3569
TscAI CASTG 6 cut(s) 1752, 2526, 2659, 2746, 3279, 3579
TseFI GTSAC 2 cut(s) 146, 1120
Tsp45I GTSAC 2 cut(s) 146, 1120
TspGWI ACGGA 6 cut(s) 663, 1807, 2414, 3496, 3921, 4082
TspRI CASTG 6 cut(s) 1752, 2526, 2659, 2746, 3279, 3579
Vha464I CTTAAG 3 cut(s) 1397, 3854, 3871
VneI GTGCAC 2 cut(s) 2745, 3129
VpaK11BI GGWCC 5 cut(s) 2100, 3179, 3202, 3421, 3830
XagI CCTNNNNNAGG 1 cut(s) 3935
XapI RAATTY 8 cut(s) 213, 646, 1030, 1105, 2092, 2821, 3610, 3758
XbaI TCTAGA 1 cut(s) 1285
XceI RCATGY 3 cut(s) 1639, 2076, 2562
XcmI CCANNNNNNNNNTGG 2 cut(s) 3637, 3658
XmiI GTMKAC 5 cut(s) 256, 1056, 2555, 3190, 3290
XmnI GAANNNNTTC 1 cut(s) 2799
XspI CTAG 9 cut(s) 317, 1286, 1313, 2121, 2577, 2661, 3560, 3566, 3677
ZrmI AGTACT 1 cut(s) 873
Zsp2I ATGCAT 3 cut(s) 2281, 2398, 3947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.