Rh7DG014100

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006366
Physical Location & Seq
Forward (+)
1174577 .. 1185114
10538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG014100.1

Sequence Viewer

Length: 3501 bp
ATGAGATACTTATTATGTCTAATGGTTTTTAAGAACCAGGTGCCCCAGCTGTTAAAGCACTCAAAGGGTGCTCCTAAAGATCATTTACGCGACACTCTGGTTCAAAAATTGGGGGTTATATTGAATCTTGCCAAATCGCTTACTTTCCCTCTGCCACTTTTAGCAGTAGCTCACCAACATCTTATTCTTGGGTCTTCAGATGGTAACACAGATGATCAAGATGCCACATTGATTAAGGTTTGGGAAAAGAAACTTGGAGTAAAAATTTCAGATGCAGCAAATGCTGAGACTTACATCCCTGAGCAATTGGCGAGCCATATTGTTGCTAAATCAGATATGGTGAAGCGGATTGGATTCATTGGTCTTGGAGCAATGGGATTCGGCATGGCAACTCAACTTCTAAAATCAAACTTTTGTGTTCTTGGTTATGATGTATATAAACCAACACAAACGCGATTTGCCAATGCTGGTGGCTTGATTGGAAGCTCTCCAGCAGAAGTATGTAAAGATGTTGATGTTCTGGTAATAATGGTCACAAATGAAGCTCAAGCTGAGAGCGCTTTATTTGGAGATTATGGTGCTATTTCAGCACTTCCATCTGGAGCTTCTATTATCCTTTCATCCACTGTTTCTCCTGGATTTGTGAGCCGAATAGATCAACGCTTACAAAATGAAGGAAAGGATCTGAAGTTGGTGGATGCTCCTGTTTCTGGTGGTGTTGTGAGAGCCGGCAATGGGACACTTACGATCATGGCATCTGGCACAGATGAAGCTCTTAAGAGTACTGGTTCAGTCCTCTCAGCATTAAGCGAGAAGCTTTATATTATCAAAGGAGGCTGTGGAGCTGGAAGTGGTGTAAAGATGGTTAATCAATTGCTAGCTGGAGTTCATATAGCATCGGGAGCTGAGGCAATGGCGTTTGGAGCTCGGTTGGGTCTTAATACAAGGATGTTATTTGATTTCATCACAAATAGTGAGGGATCATCCTGGATGTTTGAAAATCGCGTTCCACACATGCTGGACAATGATTATACACCACTTTCTGCGCTTGATATCTTTGTGAAAGATTTGGGAATTGTTACTCACGAATGTTCAATTCGAAAGGTTCCACTTCATGTATCGACTATTGCTCATCAACTATTCCTATCAGGTTCTGCTGCAGGCTGGGGTCGACAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTCACAGGCGTTAAGGTAGAAGGAAAACTTCCGGCTGTTAAGAAAGACTTTGTATTGCAATCTCTCCCCGCTGAATGGCCATTAGATCCCATAGGTGAAATACAGAAACTAAATCAGGACACATCAAAGACTTTGGTTGTTCTTGATGATGATCCTACTGGAACTCAAACTGTGCATGATATTGAGGTCTTAACTGAATGGACTGTTGAATCACTAATTGAGCAGTTCAGAAAAAGCCCGAAATGCTTTTTCATTTTGACCAACTCCAGGGCAATGAGCTCTGACAAGGCCACCACATTAATCAAAGACATCTGCACAAACTTGCATACTGCAGCCAAATCAGTTCAGGACGCTGATTATACTGTAGTTTTGAGAGGTGATTCGACTTTGCGTGGTCATTTCCCAGAGGAAGCTGATGCTGCTGTTTCAGTATTGGGTGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGCGGGCGTTATACCATCGGAGATATACATTATGTGGCTGATACTGATCAGCTTATCCCTGCAGCAGATACAGGATTTGCTAAAGATGCTGCTTTTGGCTACAAATCTTCGAATCTTCGCGAGTGGGTTGAGGAGAAAACAGCTGGTCGCATACCAGCCAGCAGTGTCGCATCCATTTCTATTCAACTGCTGAGAGAAGGTGGGCCAGATGCTGTTTGTGAGCATCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAATGCTGCTAGCGAAAGAGATATGGCTGTTTTTGCAGCTGGAATGATCAAGGCAGAACTGAAAGGCAAGCAATTCTTATGCCGCACTGCTGCTAGTTTTGTTTCTGCAAGGATTGGAATTATTCCCAAAGCTCCAATTTTGCCCAAGGATCTAGGAATAAACAAGGAACGGAATGGTGGTCTCATAGTTGTTGGATCATATGTTGCAAAGACGACAAAACAGGTAGAAGAGCTTAAGTTACAATGTGGCCAGATTCTGAGAAGCATTGAGATTTCGGTTGCTAAAGTTGCAATGAGGTCTGCAGAAGAGAGGGAGGAGGAAATCAGTACAGCAGCTGAGATGGCTGATATTTTTCTTGCAGCTCAGAATGACACACTAATAGTAACCAGTCGGGAGCTTATTACCGGCAAAAGTCCCTCTGAGAGTTTGGAAATCAACTTCAAAGTTAGCTCTGCACTGGTGGAAATAGTTAGGCGAATAACTAAAAGACCTCGTTACATCCTTGCAAAGGGTGGAATCACATCTTCTGACCTTGCTACTAAAGCCCTTGAAGCAAAATGTGCTAAAATAGTGGGACAAGCCTTGCCTGGTGTTCCCTTATGGCAGTTGGGTCCTGAAAGTAGGCATCTGGGAGTTCCATACATTGTTTTTCCCGGTAATGTTGGTGACAGTGGAGCATTGGCCGAGCTAGTGAAATCCTGGGCTCGTCCAATCAGATTTTCAACGAAAGAGCTTCTTCTGAACGCAGAAAAGGGTGGATATGCTGTCGGAGCATTCAATGTTTATAATTTAGAAGGAGTAGAGGCAGTTGTTGCTGCAGCTGAGGAACAACAGAGTCCTGCCATATTACAGATCCATCCCGGTGCCTTGAAGCAAGGAGGCATGCCGTTGATTGCTTGTTGCATCTCTGCTGCTGAACAAGCCAGTGTACCCATAACTGTACACTTTGATCATGGAACTTCAAAGCAAGATCTGGTGACAGCTCTTGAGTTGGGATTTGATTCTGTCATGGTAGATGGTTCACATCTTTCCTTTACAGAAAATGTTTCGTACACTAGGTTCATAACATTCTTGGCTCACTCAAAAGGCTTGCTAGTAGAAGCTGAACTAGGGAGATTGTCAGGGACAGAAGATGATTTGACTGTTGAAGATTATGAAGCAAGGCTGACCGATGTTAAGCAGGCTCAAGAGTTCATCGATGAAACTGGTATAGATGCTTTGGCAGTGTGTATCGGAAATGTCCATGGTAAATACCCTGCAAGTGGCCCAAATCTGAGACTTGACTTGCTTAAGGAATTGCACGCGTTGAGTTCAAAGAAAGGAGTCTTTCTGGTGCTTCATGGAGCTTCTGGTGTGCCCAAGGAACTTGTAAAGGGATGCATAGAGCTAGGTGTGAGAAAGTTCAATGTAAATACCGAGGTACGGAAAGCTTATATGGATTCCCTTAGCAACTCAAAGAAAGATCTGATCCATGTTATGGAGTCAGCAAAACTAGCCATGAAAGCAGTGATCGCAGAGAAGATGATTCTATTTGGCTCAGCAGGAAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1166

Amino Acids

125.05

Weight (kDa)

6.03

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 116 - 274 4.8e-36 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 281 - 401 7.1e-29 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 448 - 685 4.4e-58 Sugar-binding N-terminal domain
NBD_C PF17042 710 - 876 4.9e-34 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 890 - 1165 8.1e-92 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2733
AccB1I GGYRCC 2 cut(s) 40, 2810
AccB7I CCANNNNNTGG 1 cut(s) 3424
AccI GTMKAC 1 cut(s) 1171
AccII CGCG 5 cut(s) 90, 454, 1005, 1806, 3251
AciI CCGC 4 cut(s) 346, 1278, 1689, 2029
AcoI YGGCCR 3 cut(s) 1286, 2194, 2628
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 180, 707
AfaI GTAC 6 cut(s) 784, 2275, 2877, 2889, 2999, 3369
AfeI AGCGCT 1 cut(s) 559
AfiI CCNNNNNNNGG 8 cut(s) 710, 735, 1284, 1476, 2455, 3209, 3369, 3424
AflII CTTAAG 3 cut(s) 776, 2180, 3236
AflIII ACRYGT 1 cut(s) 3249
AjnI CCWGG 6 cut(s) 36, 634, 986, 1475, 2533, 2645
AjuI GAANNNNNNNTTGG 6 cut(s) 168, 200, 237, 269, 2650, 2682
Alw21I GWGCWC 3 cut(s) 73, 928, 1490
Alw26I GTCTC 4 cut(s) 281, 1199, 2132, 3217
AlwNI CAGNNNCTG 4 cut(s) 1154, 1898, 2203, 2282
Aor51HI AGCGCT 1 cut(s) 559
AoxI GGCC 6 cut(s) 1286, 1497, 1889, 2194, 2628, 3211
ApoI RAATTY 1 cut(s) 264
AseI ATTAAT 1 cut(s) 1508
AspLEI GCGC 2 cut(s) 560, 1048
AspS9I GGNCC 3 cut(s) 1889, 2558, 3212
AsuC2I CCSGG 2 cut(s) 2601, 2808
AsuHPI GGTGA 7 cut(s) 164, 352, 1316, 1598, 1658, 2624, 2935
AsuII TTCGAA 2 cut(s) 1099, 1796
AsuNHI GCTAGC 2 cut(s) 877, 1955
AvaII GGWCC 1 cut(s) 2558
BaeGI GKGCMC 2 cut(s) 45, 3306
BalI TGGCCA 2 cut(s) 1288, 2196
BanI GGYRCC 2 cut(s) 40, 2810
BanII GRGCYC 3 cut(s) 928, 1490, 2653
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 3 cut(s) 73, 928, 1490
BbvCI CCTCAGC 2 cut(s) 906, 2769
BccI CCATC 8 cut(s) 194, 604, 856, 1645, 1709, 2281, 2811, 2957
BceAI ACGGC 1 cut(s) 2818
BciT130I CCWGG 6 cut(s) 38, 636, 988, 1477, 2535, 2647
BclI TGATCA 4 cut(s) 214, 1732, 1992, 2896
BcnI CCSGG 2 cut(s) 2601, 2808
BcoDI GTCTC 4 cut(s) 281, 1199, 2132, 3217
BfmI CTRYAG 6 cut(s) 1158, 1539, 1572, 1746, 2247, 2763
BfoI RGCGCY 1 cut(s) 561
BfrI CTTAAG 3 cut(s) 776, 2180, 3236
BglII AGATCT 2 cut(s) 2917, 3409
BlpI GCTNAGC 1 cut(s) 3484
BmcAI AGTACT 1 cut(s) 784
Bme1390I CCNGG 8 cut(s) 38, 636, 988, 1477, 2535, 2601, 2647, 2808
Bme18I GGWCC 1 cut(s) 2558
BmgT120I GGNCC 3 cut(s) 1889, 2558, 3212
BmiI GGNNCC 4 cut(s) 42, 1107, 2559, 2812
BmrFI CCNGG 8 cut(s) 38, 636, 988, 1477, 2535, 2601, 2647, 2808
BmtI GCTAGC 2 cut(s) 881, 1959
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 4 cut(s) 474, 621, 903, 1459
Bpu10I CCTNAGC 4 cut(s) 300, 906, 2769, 3392
Bpu1102I GCTNAGC 1 cut(s) 3484
Bpu14I TTCGAA 2 cut(s) 1099, 1796
BpuEI CTTGAG 3 cut(s) 531, 2954, 3117
BpuMI CCSGG 2 cut(s) 2601, 2808
Bsa29I ATCGAT 1 cut(s) 3144
BsaBI GATNNNNATC 2 cut(s) 1359, 1731
BsaI GGTCTC 1 cut(s) 2132
BsaJI CCNNGG 6 cut(s) 1476, 2091, 2646, 3190, 3306, 3363
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 8 cut(s) 710, 735, 1284, 1476, 2455, 3209, 3369, 3424
Bse118I RCCGGY 2 cut(s) 728, 2351
Bse1I ACTGG 6 cut(s) 790, 1372, 2334, 2409, 2871, 3157
Bse3DI GCAATG 5 cut(s) 378, 739, 918, 1488, 2244
Bse8I GATNNNNATC 2 cut(s) 1359, 1731
BseBI CCWGG 6 cut(s) 38, 636, 988, 1477, 2535, 2647
BseCI ATCGAT 1 cut(s) 3144
BseDI CCNNGG 6 cut(s) 1476, 2091, 2646, 3190, 3306, 3363
BseJI GATNNNNATC 2 cut(s) 1359, 1731
BseLI CCNNNNNNNGG 8 cut(s) 710, 735, 1284, 1476, 2455, 3209, 3369, 3424
BseMI GCAATG 5 cut(s) 378, 739, 918, 1488, 2244
BseNI ACTGG 6 cut(s) 790, 1372, 2334, 2409, 2871, 3157
BseRI GAGGAG 2 cut(s) 1832, 2276
BseSI GKGCMC 2 cut(s) 45, 3306
BseYI CCCAGC 2 cut(s) 45, 1164
BsgI GTGCAG 2 cut(s) 1507, 2385
Bsh1236I CGCG 5 cut(s) 90, 454, 1005, 1806, 3251
BshFI GGCC 6 cut(s) 1288, 1499, 1891, 2196, 2630, 3213
BshNI GGYRCC 2 cut(s) 40, 2810
BshVI ATCGAT 1 cut(s) 3144
BsiHKAI GWGCWC 3 cut(s) 73, 928, 1490
BsiSI CCGG 5 cut(s) 729, 1241, 2352, 2601, 2808
BslFI GGGAC 4 cut(s) 751, 2346, 2535, 3085
BslI CCNNNNNNNGG 8 cut(s) 710, 735, 1284, 1476, 2455, 3209, 3369, 3424
BsmAI GTCTC 4 cut(s) 281, 1199, 2132, 3217
BsmFI GGGAC 4 cut(s) 751, 2346, 2535, 3085
BsmI GAATGC 1 cut(s) 2720
BsnI GGCC 6 cut(s) 1288, 1499, 1891, 2196, 2630, 3213
Bso31I GGTCTC 1 cut(s) 2132
Bsp119I TTCGAA 2 cut(s) 1099, 1796
Bsp1286I GDGCHC 6 cut(s) 45, 73, 928, 1490, 2653, 3306
Bsp1407I TGTACA 1 cut(s) 2887
Bsp1720I GCTNAGC 1 cut(s) 3484
Bsp19I CCATGG 1 cut(s) 3190
Bsp68I TCGCGA 1 cut(s) 1806
BspACI CCGC 4 cut(s) 346, 1278, 1689, 2029
BspANI GGCC 6 cut(s) 1288, 1499, 1891, 2196, 2630, 3213
BspDI ATCGAT 1 cut(s) 3144
BspFNI CGCG 5 cut(s) 90, 454, 1005, 1806, 3251
BspLI GGNNCC 4 cut(s) 42, 1107, 2559, 2812
BspMAI CTGCAG 5 cut(s) 1162, 1543, 1750, 2251, 2767
BspOI GCTAGC 2 cut(s) 881, 1959
BspQI GCTCTTC 1 cut(s) 2169
BspT104I TTCGAA 2 cut(s) 1099, 1796
BspT107I GGYRCC 2 cut(s) 40, 2810
BspTI CTTAAG 3 cut(s) 776, 2180, 3236
BspTNI GGTCTC 1 cut(s) 2132
BsrDI GCAATG 5 cut(s) 378, 739, 918, 1488, 2244
BsrFI RCCGGY 2 cut(s) 728, 2351
BsrGI TGTACA 1 cut(s) 2887
BsrI ACTGG 6 cut(s) 790, 1372, 2334, 2409, 2871, 3157
BssAI RCCGGY 2 cut(s) 728, 2351
BssECI CCNNGG 6 cut(s) 1476, 2091, 2646, 3190, 3306, 3363
BssT1I CCWWGG 3 cut(s) 2091, 3190, 3306
Bst2UI CCWGG 6 cut(s) 38, 636, 988, 1477, 2535, 2647
Bst4CI ACNGT 7 cut(s) 628, 1381, 1414, 1573, 2618, 2887, 3091
Bst6I CTCTTC 2 cut(s) 2169, 2247
BstAFI CTTAAG 3 cut(s) 776, 2180, 3236
BstAPI GCANNNNNTGC 3 cut(s) 281, 2507, 2759
BstAUI TGTACA 1 cut(s) 2887
BstBI TTCGAA 2 cut(s) 1099, 1796
BstDSI CCRYGG 1 cut(s) 3190
BstENI CCTNNNNNAGG 1 cut(s) 2453
BstFNI CGCG 5 cut(s) 90, 454, 1005, 1806, 3251
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 2 cut(s) 560, 1048
BstMAI GTCTC 4 cut(s) 281, 1199, 2132, 3217
BstNI CCWGG 6 cut(s) 38, 636, 988, 1477, 2535, 2647
BstNSI RCATGY 3 cut(s) 1018, 1941, 2833
BstSCI CCNGG 8 cut(s) 36, 634, 986, 1475, 2533, 2599, 2645, 2806
BstSFI CTRYAG 6 cut(s) 1158, 1539, 1572, 1746, 2247, 2763
BstSLI GKGCMC 2 cut(s) 45, 3306
BstUI CGCG 5 cut(s) 90, 454, 1005, 1806, 3251
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 6 cut(s) 682, 1294, 2095, 2799, 2917, 3409
BstYI RGATCY 6 cut(s) 682, 1294, 2095, 2799, 2917, 3409
Bsu15I ATCGAT 1 cut(s) 3144
BsuRI GGCC 6 cut(s) 1288, 1499, 1891, 2196, 2630, 3213
BsuTUI ATCGAT 1 cut(s) 3144
BtgI CCRYGG 1 cut(s) 3190
BtsI GCAGTG 4 cut(s) 1855, 2031, 3177, 3459
BtsIMutI CAGTG 8 cut(s) 624, 1855, 2031, 2402, 2623, 2878, 3177, 3459
BtuMI TCGCGA 1 cut(s) 1806
CaiI CAGNNNCTG 4 cut(s) 1154, 1898, 2203, 2282
CfoI GCGC 2 cut(s) 560, 1048
Cfr10I RCCGGY 2 cut(s) 728, 2351
Cfr13I GGNCC 3 cut(s) 1889, 2558, 3212
ClaI ATCGAT 1 cut(s) 3144
CseI GACGC 1 cut(s) 1568
CsiI ACCWGGT 1 cut(s) 36
Csp6I GTAC 6 cut(s) 783, 2274, 2876, 2888, 2998, 3368
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviQI GTAC 6 cut(s) 783, 2274, 2876, 2888, 2998, 3368
EaeI YGGCCR 3 cut(s) 1286, 2194, 2628
Eam1104I CTCTTC 2 cut(s) 2169, 2247
EarI CTCTTC 2 cut(s) 2169, 2247
Ecl136II GAGCTC 2 cut(s) 926, 1488
Eco130I CCWWGG 3 cut(s) 2091, 3190, 3306
Eco24I GRGCYC 3 cut(s) 928, 1490, 2653
Eco31I GGTCTC 1 cut(s) 2132
Eco32I GATATC 1 cut(s) 1054
Eco47I GGWCC 1 cut(s) 2558
Eco47III AGCGCT 1 cut(s) 559
Eco53kI GAGCTC 2 cut(s) 926, 1488
Eco57I CTGAAG 2 cut(s) 180, 707
EcoICRI GAGCTC 2 cut(s) 926, 1488
EcoNI CCTNNNNNAGG 1 cut(s) 2453
EcoO109I RGGNCCY 1 cut(s) 2558
EcoRII CCWGG 6 cut(s) 36, 634, 986, 1475, 2533, 2645
EcoRV GATATC 1 cut(s) 1054
EcoT14I CCWWGG 3 cut(s) 2091, 3190, 3306
EcoT22I ATGCAT 3 cut(s) 1660, 1943, 3329
EcoT38I GRGCYC 3 cut(s) 928, 1490, 2653
ErhI CCWWGG 3 cut(s) 2091, 3190, 3306
FalI AAGNNNNNCTT 8 cut(s) 1221, 1253, 1241, 1273, 2006, 2038, 2667, 2699
FaqI GGGAC 4 cut(s) 751, 2346, 2535, 3085
FauI CCCGC 2 cut(s) 1285, 1682
FauNDI CATATG 1 cut(s) 2146
FbaI TGATCA 4 cut(s) 214, 1732, 1992, 2896
FblI GTMKAC 1 cut(s) 1171
FriOI GRGCYC 3 cut(s) 928, 1490, 2653
GlaI GCGC 2 cut(s) 559, 1047
GsaI CCCAGC 2 cut(s) 49, 1168
GsuI CTGGAG 4 cut(s) 474, 621, 903, 1459
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 6 cut(s) 1288, 1499, 1891, 2196, 2630, 3213
HapII CCGG 5 cut(s) 729, 1241, 2352, 2601, 2808
HgaI GACGC 1 cut(s) 1568
HhaI GCGC 2 cut(s) 560, 1048
Hin6I GCGC 2 cut(s) 558, 1046
HinP1I GCGC 2 cut(s) 558, 1046
HincII GTYRAC 1 cut(s) 1172
HindII GTYRAC 1 cut(s) 1172
HindIII AAGCTT 2 cut(s) 815, 3375
HpaII CCGG 5 cut(s) 729, 1241, 2352, 2601, 2808
HphI GGTGA 7 cut(s) 164, 352, 1316, 1598, 1658, 2624, 2935
Hpy166II GTNNAC 5 cut(s) 1172, 2876, 2890, 2969, 3000
Hpy8I GTNNAC 5 cut(s) 1172, 2876, 2890, 2969, 3000
HpyAV CCTTC 5 cut(s) 668, 1223, 1877, 1921, 2735
HpyCH4III ACNGT 7 cut(s) 628, 1381, 1414, 1573, 2618, 2887, 3091
HspAI GCGC 2 cut(s) 558, 1046
KroI GCCGGC 1 cut(s) 728
KroNI GCCGGC 1 cut(s) 730
Ksp22I TGATCA 4 cut(s) 214, 1732, 1992, 2896
LguI GCTCTTC 1 cut(s) 2169
MabI ACCWGGT 1 cut(s) 36
MaeIII GTNAC 8 cut(s) 203, 532, 1078, 2184, 2329, 2441, 2612, 2923
MfeI CAATTG 2 cut(s) 305, 872
MflI RGATCY 6 cut(s) 682, 1294, 2095, 2799, 2917, 3409
MhlI GDGCHC 6 cut(s) 45, 73, 928, 1490, 2653, 3306
MlsI TGGCCA 2 cut(s) 1288, 2196
MluI ACGCGT 1 cut(s) 3249
MluNI TGGCCA 2 cut(s) 1288, 2196
MlyI GAGTC 3 cut(s) 2791, 3279, 3437
MmeI TCCRAC 2 cut(s) 2119, 2695
Mox20I TGGCCA 2 cut(s) 1288, 2196
Mph1103I ATGCAT 3 cut(s) 1660, 1943, 3329
MroNI GCCGGC 1 cut(s) 728
MscI TGGCCA 2 cut(s) 1288, 2196
MslI CAYNNNNRTG 1 cut(s) 2808
Msp20I TGGCCA 2 cut(s) 1288, 2196
MspA1I CMGCKG 6 cut(s) 49, 1280, 1829, 1985, 2282, 2768
MspCI CTTAAG 3 cut(s) 776, 2180, 3236
MspI CCGG 5 cut(s) 729, 1241, 2352, 2601, 2808
MspR9I CCNGG 8 cut(s) 38, 636, 988, 1477, 2535, 2601, 2647, 2808
MunI CAATTG 2 cut(s) 305, 872
Mva1269I GAATGC 1 cut(s) 2720
MvaI CCWGG 6 cut(s) 38, 636, 988, 1477, 2535, 2647
MvnI CGCG 5 cut(s) 90, 454, 1005, 1806, 3251
NaeI GCCGGC 1 cut(s) 730
NciI CCSGG 2 cut(s) 2601, 2808
NcoI CCATGG 1 cut(s) 3190
NdeI CATATG 1 cut(s) 2146
NgoMIV GCCGGC 1 cut(s) 728
NheI GCTAGC 2 cut(s) 877, 1955
NlaIV GGNNCC 4 cut(s) 42, 1107, 2559, 2812
NmeAIII GCCGAG 1 cut(s) 2656
NmuCI GTSAC 3 cut(s) 532, 2612, 2923
NruI TCGCGA 1 cut(s) 1806
NsiI ATGCAT 3 cut(s) 1660, 1943, 3329
NspI RCATGY 3 cut(s) 1018, 1941, 2833
NspV TTCGAA 2 cut(s) 1099, 1796
PaeI GCATGC 1 cut(s) 2833
PciSI GCTCTTC 1 cut(s) 2169
PctI GAATGC 1 cut(s) 2720
PdiI GCCGGC 1 cut(s) 730
PflMI CCANNNNNTGG 1 cut(s) 3424
PfoI TCCNGGA 2 cut(s) 634, 986
PleI GAGTC 3 cut(s) 2790, 3278, 3436
PpsI GAGTC 3 cut(s) 2790, 3278, 3436
PpuMI RGGWCCY 1 cut(s) 2558
PshBI ATTAAT 1 cut(s) 1508
PsiI TTATAA 1 cut(s) 2733
Psp124BI GAGCTC 2 cut(s) 928, 1490
Psp5II RGGWCCY 1 cut(s) 2558
Psp6I CCWGG 6 cut(s) 36, 634, 986, 1475, 2533, 2645
PspFI CCCAGC 2 cut(s) 45, 1164
PspGI CCWGG 6 cut(s) 36, 634, 986, 1475, 2533, 2645
PspN4I GGNNCC 4 cut(s) 42, 1107, 2559, 2812
PspPI GGNCC 3 cut(s) 1889, 2558, 3212
PspPPI RGGWCCY 1 cut(s) 2558
PsrI GAACNNNNNNTAC 2 cut(s) 2990, 3022
PstI CTGCAG 5 cut(s) 1162, 1543, 1750, 2251, 2767
PstNI CAGNNNCTG 4 cut(s) 1154, 1898, 2203, 2282
PsuI RGATCY 6 cut(s) 682, 1294, 2095, 2799, 2917, 3409
PvuII CAGCTG 5 cut(s) 49, 1829, 1985, 2282, 2768
RruI TCGCGA 1 cut(s) 1806
RsaI GTAC 6 cut(s) 784, 2275, 2877, 2889, 2999, 3369
RsaNI GTAC 6 cut(s) 783, 2274, 2876, 2888, 2998, 3368
RseI CAYNNNNRTG 1 cut(s) 2808
SacI GAGCTC 2 cut(s) 928, 1490
SalI GTCGAC 1 cut(s) 1170
SapI GCTCTTC 1 cut(s) 2169
Sau96I GGNCC 3 cut(s) 1889, 2558, 3212
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 3 cut(s) 2791, 3279, 3437
ScrFI CCNGG 8 cut(s) 38, 636, 988, 1477, 2535, 2601, 2647, 2808
SduI GDGCHC 6 cut(s) 45, 73, 928, 1490, 2653, 3306
SexAI ACCWGGT 1 cut(s) 36
SfcI CTRYAG 6 cut(s) 1158, 1539, 1572, 1746, 2247, 2763
SfuI TTCGAA 2 cut(s) 1099, 1796
SinI GGWCC 1 cut(s) 2558
SmiMI CAYNNNNRTG 1 cut(s) 2808
SmlI CTYRAG 6 cut(s) 546, 776, 2180, 2933, 3132, 3236
SmoI CTYRAG 6 cut(s) 546, 776, 2180, 2933, 3132, 3236
SphI GCATGC 1 cut(s) 2833
SsiI CCGC 4 cut(s) 346, 1278, 1689, 2029
SstI GAGCTC 2 cut(s) 928, 1490
StyD4I CCNGG 8 cut(s) 36, 634, 986, 1475, 2533, 2599, 2645, 2806
StyI CCWWGG 3 cut(s) 2091, 3190, 3306
TaaI ACNGT 7 cut(s) 628, 1381, 1414, 1573, 2618, 2887, 3091
TaqI TCGA 6 cut(s) 1099, 1121, 1171, 1592, 1796, 3144
TaqII GACCGA 1 cut(s) 3131
TatI WGTACW 3 cut(s) 782, 2273, 2887
TauI GCSGC 1 cut(s) 2031
TscAI CASTG 8 cut(s) 631, 1855, 2038, 2409, 2623, 2878, 3177, 3459
TseFI GTSAC 3 cut(s) 532, 2612, 2923
Tsp45I GTSAC 3 cut(s) 532, 2612, 2923
TspGWI ACGGA 2 cut(s) 2131, 3385
TspRI CASTG 8 cut(s) 631, 1855, 2038, 2409, 2623, 2878, 3177, 3459
Van91I CCANNNNNTGG 1 cut(s) 3424
Vha464I CTTAAG 3 cut(s) 776, 2180, 3236
VpaK11BI GGWCC 1 cut(s) 2558
VspI ATTAAT 1 cut(s) 1508
XagI CCTNNNNNAGG 1 cut(s) 2453
XapI RAATTY 1 cut(s) 264
XceI RCATGY 3 cut(s) 1018, 1941, 2833
XmiI GTMKAC 1 cut(s) 1171
ZrmI AGTACT 1 cut(s) 784
Zsp2I ATGCAT 3 cut(s) 1660, 1943, 3329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.