Rh7BG013900

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006366
Physical Location & Seq
Forward (+)
892936 .. 906316
13381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG013900.1

Sequence Viewer

Length: 3762 bp
ATGGCGTTTCATGGTGTTGTTGGGTTTGTGGGATTGGATGGCTTGAGCTTGGACTTGGCTTCTTCACTTCTCCGCAGCGGTTACAGAGTTCAAGCATTTGAGACATATGAACCTTTGATAAATGAGTTTTTAAAGCTAGGGGGAACAAGATGTAGCAGCCCAAAGGAGGCAGGAAAAGATGTTTCAGCTTTGGTTCTGTTAACTTCTCAGGCAGATCAAATTAATGATGCAACATCAGGATTGCAGAACGATACTGTTCTCATTTTTAATTCGACAATGTTACCGTTGTACATTAAGAATTTGCAGACGTGTTTCACAGCTGATTATAAGCCAGCTTATGTTGTAGATGTATATGCCATGAAGGCAGTATCTGATAGTTTAAATGGAAAAATCATGATTGCTTCTTCAGGAAGTTCAAATGCCATTTTAAAGGCACGACCAGTTCTCTCTGCAATGTGTGAAAAGCTTTATGTTTTTGAGGGTGAAGTTGGTGCTGGAAGCAAAATTAAGATGGTTAAGGAACTGCTTGAGGGTATTCATCTTGTTGCATCTTTGGAAGCTATTTCTCTTGGTACCAAAGCTGGTATTCATCCGTGGATAATCTATGATATCATATCTAATGCGGCCGGAAACTCATGGGTTTTTAAGAACCAGGTGCCACAGCTGTTAAAGCACTCAAAGGGTGCTCCTAAAGATCATTTACGCGACACTCTGGTTCAAAAATTGGGGGTTATATTGAATCTTGCCAAATCACTTACTTTCCCTCTGCCACTTTTAGCAGTAGCTCACCAACAACTTATTCTTGGGTCTTCAGATGGTAACACAGATGATCAAGATGCCACATTGATTAAGGTTTGGGAAAAGAAACTTGGAGTAAAAATTTCAGATGCAGCAAATGCTGAGACTTACATCCCTGAGCAATTGGCGAGCCATATTGTTGCTAAATCAGATATGGTGAAGCGGATTGGATTCATTGGTCTTGGAGCAATGGGATTCGGCATGGCAACTCAACTTCTAAAATCAAACTTTTGTGTTCTTGGTTATGATGTATATAAACCAACACTAACGCAATTTGCCAATGCTGGTGGCTTGATTGGAAGCTCTCCAGCAGAAGTATGTAAAGATGTTGATGTTCTGGTAATAATGGTCACAAATGAAGCTCAAGCTGAGAGCGCTTTATTTGGAGATTTTGGTGCTATTTCAGCACTTCCATCTGGAGCTTCTATTATCCTTTCATCCACTGTTTCTCCTGGATTTGTGAGCCGACTAGATCAACGCTTACAAAATGAAGGAAAGGATTTGAAGTTGGTGGATGCTCCTGTTTCTGGTGGTGTTGTGAGAGCTGGCAATGGGACACTTACGATCATGGCATCTGGCACAGATGAAGCTCTTAAGAGTACTGGTTCAGTCCTCTCAGCATTAAGCGAGAAGCTTTATATTATCAAAGGAGGCTGTGGAGCTGGAAGTGGTGTAAAGATGGTTAATCAATTGCTAGCTGGAGTTCATATAGCATCGGGAGCTGAGGCAATGGCGTTTGGAGCTCGGTTGGGTCTTAATACAAGGATGTTATTTGATTTCATCACAAATAGTGAGGGATCATCCTGGATGTTTGAAAATCGTGTTCCACACATGCTGGACAATGATTATACACCACTTTCTGCGCTTGATATCTTTGTGAAAGATTTGGGAATTGTTACTCACGAATGTTCAATTCGAAAGGTTCCACTTCATGTATCGACTATTGCTCATCAACTATTCCTATCAGGTTCTGCTGCAGGCTGGGGTCGACAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTCACAGGCGTTAAGGTAGAAGGAAAACTTCCGGCTGTTAAGAAAGACTTTGTATTGCAATCTCTCCCAGCTGAATGGCCATTAGATCCCATAGGTGAAATACAGAAACTAAATCAGGACACATCAAAGACTTTGGTTGTTCTTGATGATGATCCGACTGGAACTCAAACTGTGCATGATATTGAGGTCTTAACTGAATGGACTGTTGAATCACTAATTGAGCAGTTCAGAAAAAGCCCGAAATGCTTTTTCATTTTGACCAACTCCAGGGCAATGAGCTCTGACAAGGCCACCACATTAATCAAAGACATCTGCACAAACTTGCATACTGCAGCCAAATCAGTTCAGGACGCTGATTATACTGTAGTTTTGAGAGGTGATTCGACTTTGCGTGGTCATTTCCCAGAGGAAGCTGATGCTGCTGTTTCAGTATTGGGTGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGCGGGCGTTATACCATCGGAGATATACATTATGTGGCTGATACTGATCAGCTTATCCCTGCAGCAGATACAGGATTTGCTAAAGATGCTGCTTTTGGCTACAAATCTTCGAATCTTCGCGAGTGGGTTGAGGAGAAAACAGCTGGTCGCATACCAGCCAGCAGTGTCGCATCCATTTCTATTCAACTGCTGAGAGAAGGTGGGCCAGATGCTGTTTGTGAGCATCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAATGCTGCTAGCGAAAGAGATATGGCTGTTTTTGCAGCTGGAATGATCAAGGCAGAACTGAAAGGCAAGCAATTCTTATGCCGCACTGCTGCTAGTTTTGTTTCTGCAAGGATTGGAATTATTCCCAAAGCTCCAATTTTGCCCAAGGATCTAGGAATAAACAAGGAACGGAATGGTGGTCTCATAGTTGTTGGATCATATGTTGCAAAGACGACAAAACAGGTAGAAGAGCTTAAGTTACAATGTGGCCAGATTCTGAGAAGCATTGAGGTTTCGGTTGCTAAAGTTGCAATGAAGTCTGCAGAAGAGAGGGAGGAGGAAATCAGTACAGCAGCTGAGATGGCTGATATTTTTCTTGAAGCTCAGAATGACACACTAATAGTAACCAGTCGGGAGCTTATTACCGGCAAAAGTCCCTCTGAGAGTTTGGAAATCAACTTCAAAGTTAGCTCTGCACTGGTGGAAATAGTTAGGCGAATAACTAAAAGACCTCGTTACATCCTTGCAAAGGGTGGAATCACATCTTCTGACCTTGCTACTAAAGCCCTTGAAGCAAAATGTGCTAAAATAGTGGGACAAGCCTTGCCTGGTGTTCCCTTATGGCAGTTGGGTCCTGAAAGTAGGCATCTGGGAGTTCCATACATTGTTTTTCCCGGTAATGTTGGTGACAGTGGAGCATTGGCCGAGCTAGTGAAATCCTGGGCTCGTCCAATCAGATTTTCAACGAAAGAGCTTCTTCTGAACGCAGAAAAGGGTGGATATGCTGTTGGAGCATTCAATGTTTATAATTTAGAAGGAGTAGAGGCAGTTGTTGCTGCAGCGGAGGAACAACAGAGTCCTGCCATATTACAGATCCATCCCGGTGCCTTGAAGCAAGGAGGCATGCCGTTGATTGCTTGTTGCATCTCTGCTGCTGAACAAGCCAGTGTACCCATAACTGTACACTTTGATCATGGAACTTCAAAGCAAGATCTGGTGACAGCTCTTGAGTTGGGATTCGATTCTGTCATGGTAGATGGTTCACATCTTTCCTTTACAGAAAATGTTTCTTACACTAGGTTCATAACATTCTTGGCTCACTCAAAAGGCTTGCTAGTAGAAGCTGAACTAGGGAGATTGTCAGGGACAGAAGATGATTTGACTGTTGAAGATTATGAAGCAAGGCTGACCGATGTTAAGCAGGTTATTTTTTCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1253

Amino Acids

134.16

Weight (kDa)

5.56

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 7 - 157 6.2e-07 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 164 - 285 2.9e-18 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 321 - 479 4.2e-36 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 486 - 606 7.8e-29 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 653 - 890 5e-58 Sugar-binding N-terminal domain
NBD_C PF17042 915 - 1081 4.1e-34 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1095 - 1249 1.4e-48 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 327, 3348
Acc36I ACCTGC 1 cut(s) 3733
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 3 cut(s) 572, 655, 3425
AccI GTMKAC 1 cut(s) 1786
AccII CGCG 2 cut(s) 705, 2421
AciI CCGC 7 cut(s) 73, 78, 623, 961, 2304, 2644, 3383
AclWI GGATC 8 cut(s) 1603, 1904, 1970, 2285, 2554, 2718, 2764, 3409
AcoI YGGCCR 4 cut(s) 624, 1901, 2809, 3243
AcsI RAATTY 2 cut(s) 298, 879
AcuI CTGAAG 2 cut(s) 390, 795
AfaI GTAC 6 cut(s) 290, 574, 1399, 2890, 3492, 3504
AfeI AGCGCT 1 cut(s) 1174
AfiI CCNNNNNNNGG 4 cut(s) 166, 1325, 2091, 3070
AflII CTTAAG 2 cut(s) 1391, 2795
AflIII ACRYGT 1 cut(s) 308
AjiI CACGTC 1 cut(s) 309
AjnI CCWGG 6 cut(s) 651, 1249, 1601, 2090, 3148, 3260
AjuI GAANNNNNNNTTGG 6 cut(s) 783, 815, 852, 884, 3265, 3297
Alw21I GWGCWC 3 cut(s) 688, 1543, 2105
Alw26I GTCTC 4 cut(s) 95, 896, 1814, 2747
AlwI GGATC 8 cut(s) 1603, 1904, 1970, 2285, 2554, 2718, 2764, 3409
AlwNI CAGNNNCTG 5 cut(s) 371, 1769, 2513, 2818, 2897
Aor51HI AGCGCT 1 cut(s) 1174
AoxI GGCC 6 cut(s) 624, 1901, 2112, 2504, 2809, 3243
ApoI RAATTY 2 cut(s) 298, 879
AseI ATTAAT 2 cut(s) 222, 2123
Asp718I GGTACC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 1175, 1663
AspS9I GGNCC 2 cut(s) 2504, 3173
AsuC2I CCSGG 2 cut(s) 3216, 3423
AsuHPI GGTGA 8 cut(s) 494, 779, 967, 1931, 2213, 2273, 3239, 3550
AsuII TTCGAA 2 cut(s) 1714, 2411
AsuNHI GCTAGC 2 cut(s) 1492, 2570
AvaII GGWCC 1 cut(s) 3173
BalI TGGCCA 2 cut(s) 1903, 2811
BanI GGYRCC 3 cut(s) 572, 655, 3425
BanII GRGCYC 3 cut(s) 1543, 2105, 3268
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 3 cut(s) 688, 1543, 2105
BbvCI CCTCAGC 1 cut(s) 1521
BceAI ACGGC 1 cut(s) 3433
BciT130I CCWGG 6 cut(s) 653, 1251, 1603, 2092, 3150, 3262
BclI TGATCA 4 cut(s) 829, 2347, 2607, 3511
BcnI CCSGG 2 cut(s) 3216, 3423
BcoDI GTCTC 4 cut(s) 95, 896, 1814, 2747
BfmI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
BfoI RGCGCY 1 cut(s) 1176
BfrI CTTAAG 2 cut(s) 1391, 2795
BfuAI ACCTGC 1 cut(s) 3733
BglI GCCNNNNNGGC 1 cut(s) 362
BglII AGATCT 1 cut(s) 3532
BmcAI AGTACT 1 cut(s) 1399
Bme1390I CCNGG 8 cut(s) 653, 1251, 1603, 2092, 3150, 3216, 3262, 3423
Bme18I GGWCC 1 cut(s) 3173
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 2 cut(s) 2504, 3173
BmiI GGNNCC 5 cut(s) 574, 657, 1722, 3174, 3427
BmrFI CCNGG 8 cut(s) 653, 1251, 1603, 2092, 3150, 3216, 3262, 3423
BmtI GCTAGC 2 cut(s) 1496, 2574
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 4 cut(s) 1089, 1236, 1518, 2074
Bpu10I CCTNAGC 2 cut(s) 915, 1521
Bpu14I TTCGAA 2 cut(s) 1714, 2411
BpuEI CTTGAG 4 cut(s) 64, 548, 1146, 3569
BpuMI CCSGG 2 cut(s) 3216, 3423
BsaBI GATNNNNATC 2 cut(s) 1974, 2346
BsaI GGTCTC 1 cut(s) 2747
BsaJI CCNNGG 4 cut(s) 593, 2091, 2706, 3261
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 1325, 2091, 3070
Bse118I RCCGGY 1 cut(s) 2966
Bse1I ACTGG 6 cut(s) 440, 1405, 1987, 2949, 3024, 3486
Bse3DI GCAATG 6 cut(s) 459, 993, 1354, 1533, 2103, 2859
Bse8I GATNNNNATC 2 cut(s) 1974, 2346
BseBI CCWGG 6 cut(s) 653, 1251, 1603, 2092, 3150, 3262
BseDI CCNNGG 4 cut(s) 593, 2091, 2706, 3261
BseJI GATNNNNATC 2 cut(s) 1974, 2346
BseLI CCNNNNNNNGG 4 cut(s) 166, 1325, 2091, 3070
BseMI GCAATG 6 cut(s) 459, 993, 1354, 1533, 2103, 2859
BseNI ACTGG 6 cut(s) 440, 1405, 1987, 2949, 3024, 3486
BseRI GAGGAG 2 cut(s) 2447, 2891
BseX3I CGGCCG 1 cut(s) 624
BseYI CCCAGC 2 cut(s) 1779, 1891
BsgI GTGCAG 2 cut(s) 2122, 3000
Bsh1236I CGCG 2 cut(s) 705, 2421
Bsh1285I CGRYCG 1 cut(s) 627
BshFI GGCC 6 cut(s) 626, 1903, 2114, 2506, 2811, 3245
BshNI GGYRCC 3 cut(s) 572, 655, 3425
BsiEI CGRYCG 1 cut(s) 627
BsiHKAI GWGCWC 3 cut(s) 688, 1543, 2105
BsiSI CCGG 5 cut(s) 627, 1856, 2967, 3216, 3423
BslFI GGGAC 4 cut(s) 1366, 2961, 3150, 3700
BslI CCNNNNNNNGG 4 cut(s) 166, 1325, 2091, 3070
BsmAI GTCTC 4 cut(s) 95, 896, 1814, 2747
BsmFI GGGAC 4 cut(s) 1366, 2961, 3150, 3700
BsmI GAATGC 1 cut(s) 3335
BsnI GGCC 6 cut(s) 626, 1903, 2114, 2506, 2811, 3245
Bso31I GGTCTC 1 cut(s) 2747
Bsp119I TTCGAA 2 cut(s) 1714, 2411
Bsp1286I GDGCHC 4 cut(s) 688, 1543, 2105, 3268
Bsp1407I TGTACA 2 cut(s) 288, 3502
Bsp68I TCGCGA 1 cut(s) 2421
BspACI CCGC 7 cut(s) 73, 78, 623, 961, 2304, 2644, 3383
BspANI GGCC 6 cut(s) 626, 1903, 2114, 2506, 2811, 3245
BspFNI CGCG 2 cut(s) 705, 2421
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 5 cut(s) 574, 657, 1722, 3174, 3427
BspMAI CTGCAG 5 cut(s) 1777, 2158, 2365, 2866, 3382
BspMI ACCTGC 1 cut(s) 3733
BspOI GCTAGC 2 cut(s) 1496, 2574
BspPI GGATC 8 cut(s) 1603, 1904, 1970, 2285, 2554, 2718, 2764, 3409
BspQI GCTCTTC 1 cut(s) 2784
BspT104I TTCGAA 2 cut(s) 1714, 2411
BspT107I GGYRCC 3 cut(s) 572, 655, 3425
BspTI CTTAAG 2 cut(s) 1391, 2795
BspTNI GGTCTC 1 cut(s) 2747
BsrDI GCAATG 6 cut(s) 459, 993, 1354, 1533, 2103, 2859
BsrFI RCCGGY 1 cut(s) 2966
BsrGI TGTACA 2 cut(s) 288, 3502
BsrI ACTGG 6 cut(s) 440, 1405, 1987, 2949, 3024, 3486
BssAI RCCGGY 1 cut(s) 2966
BssECI CCNNGG 4 cut(s) 593, 2091, 2706, 3261
BssT1I CCWWGG 1 cut(s) 2706
Bst2UI CCWGG 6 cut(s) 653, 1251, 1603, 2092, 3150, 3262
Bst4CI ACNGT 9 cut(s) 256, 285, 1243, 1996, 2029, 2188, 3233, 3502, 3706
Bst6I CTCTTC 2 cut(s) 2784, 2862
BstAFI CTTAAG 2 cut(s) 1391, 2795
BstAPI GCANNNNNTGC 3 cut(s) 896, 3122, 3374
BstAUI TGTACA 2 cut(s) 288, 3502
BstBI TTCGAA 2 cut(s) 1714, 2411
BstDSI CCRYGG 1 cut(s) 593
BstENI CCTNNNNNAGG 1 cut(s) 3068
BstFNI CGCG 2 cut(s) 705, 2421
BstH2I RGCGCY 1 cut(s) 1176
BstHHI GCGC 2 cut(s) 1175, 1663
BstMAI GTCTC 4 cut(s) 95, 896, 1814, 2747
BstMCI CGRYCG 1 cut(s) 627
BstNI CCWGG 6 cut(s) 653, 1251, 1603, 2092, 3150, 3262
BstNSI RCATGY 3 cut(s) 1633, 2556, 3448
BstSCI CCNGG 8 cut(s) 651, 1249, 1601, 2090, 3148, 3214, 3260, 3421
BstSFI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
BstUI CGCG 2 cut(s) 705, 2421
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 4 cut(s) 1909, 2710, 3414, 3532
BstXI CCANNNNNNTGG 1 cut(s) 1899
BstYI RGATCY 4 cut(s) 1909, 2710, 3414, 3532
BstZI CGGCCG 1 cut(s) 624
BsuRI GGCC 6 cut(s) 626, 1903, 2114, 2506, 2811, 3245
BtgI CCRYGG 1 cut(s) 593
BtrI CACGTC 1 cut(s) 309
BtsI GCAGTG 2 cut(s) 2470, 2646
BtsIMutI CAGTG 6 cut(s) 1239, 2470, 2646, 3017, 3238, 3493
BtuMI TCGCGA 1 cut(s) 2421
BveI ACCTGC 1 cut(s) 3733
CaiI CAGNNNCTG 5 cut(s) 371, 1769, 2513, 2818, 2897
CciI TCATGA 1 cut(s) 393
CfoI GCGC 2 cut(s) 1175, 1663
Cfr10I RCCGGY 1 cut(s) 2966
Cfr13I GGNCC 2 cut(s) 2504, 3173
CseI GACGC 1 cut(s) 2183
CsiI ACCWGGT 1 cut(s) 651
Csp6I GTAC 6 cut(s) 289, 573, 1398, 2889, 3491, 3503
CspCI CAANNNNNGTGG 2 cut(s) 1066, 1101
CviQI GTAC 6 cut(s) 289, 573, 1398, 2889, 3491, 3503
DraI TTTAAA 3 cut(s) 132, 381, 429
EaeI YGGCCR 4 cut(s) 624, 1901, 2809, 3243
EagI CGGCCG 1 cut(s) 624
Eam1104I CTCTTC 2 cut(s) 2784, 2862
EarI CTCTTC 2 cut(s) 2784, 2862
Ecl136II GAGCTC 2 cut(s) 1541, 2103
EclXI CGGCCG 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 2706
Eco24I GRGCYC 3 cut(s) 1543, 2105, 3268
Eco31I GGTCTC 1 cut(s) 2747
Eco32I GATATC 2 cut(s) 610, 1669
Eco47I GGWCC 1 cut(s) 3173
Eco47III AGCGCT 1 cut(s) 1174
Eco52I CGGCCG 1 cut(s) 624
Eco53kI GAGCTC 2 cut(s) 1541, 2103
Eco57I CTGAAG 2 cut(s) 390, 795
EcoICRI GAGCTC 2 cut(s) 1541, 2103
EcoNI CCTNNNNNAGG 1 cut(s) 3068
EcoO109I RGGNCCY 1 cut(s) 3173
EcoRII CCWGG 6 cut(s) 651, 1249, 1601, 2090, 3148, 3260
EcoRV GATATC 2 cut(s) 610, 1669
EcoT14I CCWWGG 1 cut(s) 2706
EcoT22I ATGCAT 2 cut(s) 2275, 2558
EcoT38I GRGCYC 3 cut(s) 1543, 2105, 3268
ErhI CCWWGG 1 cut(s) 2706
FalI AAGNNNNNCTT 8 cut(s) 1836, 1868, 1856, 1888, 2621, 2653, 3282, 3314
FaqI GGGAC 4 cut(s) 1366, 2961, 3150, 3700
FauI CCCGC 1 cut(s) 2297
FauNDI CATATG 2 cut(s) 106, 2761
FbaI TGATCA 4 cut(s) 829, 2347, 2607, 3511
FblI GTMKAC 1 cut(s) 1786
FriOI GRGCYC 3 cut(s) 1543, 2105, 3268
GlaI GCGC 2 cut(s) 1174, 1662
GsaI CCCAGC 2 cut(s) 1783, 1895
GsuI CTGGAG 4 cut(s) 1089, 1236, 1518, 2074
HaeII RGCGCY 1 cut(s) 1176
HaeIII GGCC 6 cut(s) 626, 1903, 2114, 2506, 2811, 3245
HapII CCGG 5 cut(s) 627, 1856, 2967, 3216, 3423
HgaI GACGC 1 cut(s) 2183
HhaI GCGC 2 cut(s) 1175, 1663
Hin6I GCGC 2 cut(s) 1173, 1661
HinP1I GCGC 2 cut(s) 1173, 1661
HincII GTYRAC 2 cut(s) 201, 1787
HindII GTYRAC 2 cut(s) 201, 1787
HindIII AAGCTT 2 cut(s) 464, 1430
HpaI GTTAAC 1 cut(s) 201
HpaII CCGG 5 cut(s) 627, 1856, 2967, 3216, 3423
HphI GGTGA 8 cut(s) 494, 779, 967, 1931, 2213, 2273, 3239, 3550
Hpy166II GTNNAC 5 cut(s) 201, 1787, 3491, 3505, 3584
Hpy8I GTNNAC 5 cut(s) 201, 1787, 3491, 3505, 3584
HpyAV CCTTC 6 cut(s) 355, 1283, 1838, 2492, 2536, 3350
HpyCH4III ACNGT 9 cut(s) 256, 285, 1243, 1996, 2029, 2188, 3233, 3502, 3706
HpyCH4IV ACGT 1 cut(s) 308
HpySE526I ACGT 1 cut(s) 308
HspAI GCGC 2 cut(s) 1173, 1661
KpnI GGTACC 1 cut(s) 576
Ksp22I TGATCA 4 cut(s) 829, 2347, 2607, 3511
KspAI GTTAAC 1 cut(s) 201
LguI GCTCTTC 1 cut(s) 2784
MabI ACCWGGT 1 cut(s) 651
MaeII ACGT 1 cut(s) 308
MfeI CAATTG 2 cut(s) 920, 1487
MflI RGATCY 4 cut(s) 1909, 2710, 3414, 3532
MhlI GDGCHC 4 cut(s) 688, 1543, 2105, 3268
MlsI TGGCCA 2 cut(s) 1903, 2811
MluNI TGGCCA 2 cut(s) 1903, 2811
MlyI GAGTC 1 cut(s) 3406
MmeI TCCRAC 3 cut(s) 2003, 2734, 3310
Mox20I TGGCCA 2 cut(s) 1903, 2811
Mph1103I ATGCAT 2 cut(s) 2275, 2558
MscI TGGCCA 2 cut(s) 1903, 2811
MslI CAYNNNNRTG 1 cut(s) 3423
Msp20I TGGCCA 2 cut(s) 1903, 2811
MspA1I CMGCKG 8 cut(s) 78, 320, 664, 1895, 2444, 2600, 2897, 3383
MspCI CTTAAG 2 cut(s) 1391, 2795
MspI CCGG 5 cut(s) 627, 1856, 2967, 3216, 3423
MspR9I CCNGG 8 cut(s) 653, 1251, 1603, 2092, 3150, 3216, 3262, 3423
MunI CAATTG 2 cut(s) 920, 1487
Mva1269I GAATGC 1 cut(s) 3335
MvaI CCWGG 6 cut(s) 653, 1251, 1603, 2092, 3150, 3262
MvnI CGCG 2 cut(s) 705, 2421
NciI CCSGG 2 cut(s) 3216, 3423
NdeI CATATG 2 cut(s) 106, 2761
NheI GCTAGC 2 cut(s) 1492, 2570
NlaIV GGNNCC 5 cut(s) 574, 657, 1722, 3174, 3427
NmeAIII GCCGAG 1 cut(s) 3271
NmuCI GTSAC 3 cut(s) 1147, 3227, 3538
NruI TCGCGA 1 cut(s) 2421
NsiI ATGCAT 2 cut(s) 2275, 2558
NspI RCATGY 3 cut(s) 1633, 2556, 3448
NspV TTCGAA 2 cut(s) 1714, 2411
PaeI GCATGC 1 cut(s) 3448
PagI TCATGA 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 2784
PctI GAATGC 1 cut(s) 3335
PfoI TCCNGGA 2 cut(s) 1249, 1601
PleI GAGTC 1 cut(s) 3405
PpsI GAGTC 1 cut(s) 3405
PpuMI RGGWCCY 1 cut(s) 3173
PshBI ATTAAT 2 cut(s) 222, 2123
PsiI TTATAA 2 cut(s) 327, 3348
Psp124BI GAGCTC 2 cut(s) 1543, 2105
Psp5II RGGWCCY 1 cut(s) 3173
Psp6I CCWGG 6 cut(s) 651, 1249, 1601, 2090, 3148, 3260
PspFI CCCAGC 2 cut(s) 1779, 1891
PspGI CCWGG 6 cut(s) 651, 1249, 1601, 2090, 3148, 3260
PspN4I GGNNCC 5 cut(s) 574, 657, 1722, 3174, 3427
PspPI GGNCC 2 cut(s) 2504, 3173
PspPPI RGGWCCY 1 cut(s) 3173
PstI CTGCAG 5 cut(s) 1777, 2158, 2365, 2866, 3382
PstNI CAGNNNCTG 5 cut(s) 371, 1769, 2513, 2818, 2897
PsuI RGATCY 4 cut(s) 1909, 2710, 3414, 3532
PvuII CAGCTG 6 cut(s) 320, 664, 1895, 2444, 2600, 2897
RruI TCGCGA 1 cut(s) 2421
RsaI GTAC 6 cut(s) 290, 574, 1399, 2890, 3492, 3504
RsaNI GTAC 6 cut(s) 289, 573, 1398, 2889, 3491, 3503
RseI CAYNNNNRTG 1 cut(s) 3423
SacI GAGCTC 2 cut(s) 1543, 2105
SalI GTCGAC 1 cut(s) 1785
SapI GCTCTTC 1 cut(s) 2784
Sau96I GGNCC 2 cut(s) 2504, 3173
ScaI AGTACT 1 cut(s) 1399
SchI GAGTC 1 cut(s) 3406
ScrFI CCNGG 8 cut(s) 653, 1251, 1603, 2092, 3150, 3216, 3262, 3423
SduI GDGCHC 4 cut(s) 688, 1543, 2105, 3268
SexAI ACCWGGT 1 cut(s) 651
SfcI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
SfuI TTCGAA 2 cut(s) 1714, 2411
SinI GGWCC 1 cut(s) 3173
SmiMI CAYNNNNRTG 1 cut(s) 3423
SmlI CTYRAG 6 cut(s) 43, 527, 1161, 1391, 2795, 3548
SmoI CTYRAG 6 cut(s) 43, 527, 1161, 1391, 2795, 3548
SphI GCATGC 1 cut(s) 3448
SsiI CCGC 7 cut(s) 73, 78, 623, 961, 2304, 2644, 3383
SstI GAGCTC 2 cut(s) 1543, 2105
StyD4I CCNGG 8 cut(s) 651, 1249, 1601, 2090, 3148, 3214, 3260, 3421
StyI CCWWGG 1 cut(s) 2706
TaaI ACNGT 9 cut(s) 256, 285, 1243, 1996, 2029, 2188, 3233, 3502, 3706
TaiI ACGT 1 cut(s) 311
TaqI TCGA 7 cut(s) 272, 1714, 1736, 1786, 2207, 2411, 3561
TaqII GACCGA 1 cut(s) 3746
TatI WGTACW 4 cut(s) 288, 1397, 2888, 3502
TauI GCSGC 2 cut(s) 626, 2646
TscAI CASTG 6 cut(s) 1246, 2470, 2653, 3024, 3238, 3493
TseFI GTSAC 3 cut(s) 1147, 3227, 3538
Tsp45I GTSAC 3 cut(s) 1147, 3227, 3538
TspGWI ACGGA 2 cut(s) 582, 2746
TspRI CASTG 6 cut(s) 1246, 2470, 2653, 3024, 3238, 3493
Vha464I CTTAAG 2 cut(s) 1391, 2795
VpaK11BI GGWCC 1 cut(s) 3173
VspI ATTAAT 2 cut(s) 222, 2123
XagI CCTNNNNNAGG 1 cut(s) 3068
XapI RAATTY 2 cut(s) 298, 879
XceI RCATGY 3 cut(s) 1633, 2556, 3448
XmiI GTMKAC 1 cut(s) 1786
ZrmI AGTACT 1 cut(s) 1399
Zsp2I ATGCAT 2 cut(s) 2275, 2558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.