FvH4_5g17450

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006366
Physical Location & Seq
Reverse (-)
9977534 .. 9990742
13209 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17450.t1

Sequence Viewer

Length: 4116 bp
ATGGCGTTTCGAGGTGTTGTTGGGTTTGTGGGCTTGGATGACCTCAGCTTGGAGTTGGCTTCTTCACTTCTCCGCTGCCGTTACAAAGTTCAAGCTTTTGAGACATATGAACCATTGATAAATGAGTTTCTAAAGCTAGGTGGGACCAGATGTGGCAGCCCAAAGGAGGTAGGGAAAGATGTTTCAGCTTTGATTCTGTTAACTTCTCAGGCAGATCAAATTAATGACGCAACGATAGGAATGCAGAAGGATACTGTTCTCATTTTTAATTCAACGTTGTTACCATTGTACATTAAGAATTTGCAGACGTGTTTCACAGCTGATTATAAGCCAGCTTATGTTGTAGATGTATATGCCACGAAGGCAGTATCTGATAGTTTAAATGGAAAAATCATGATTGCTTCTTCAGGAAGTTCAGACGCCATTTTAAAGGCACGACCAGTTCTCTCTGCAATGTGTGAAAAGCTTTATGTTTTTGAGGGTGAAGTTGGTGCTGGAAGCAAAATTAAGATGGTTAAGGAACTGCTTGAGGGTATTCATCTTGTTGCTTCTTTGGAAGCTATTTCTCTTGGTACCAAAGCTGGTGTTCATCCGTGGATAATCTATGATATCATATCTAATGCGGCCGGAAACTCATGGGTGTTCAAGAACCATATGCCTCAGCTTTTGAAGCCCTTAAAGGGTGCTCCTGAAGATCATTTACCCAACACTTTTGCTCAAAATATGAGAAATATATTGGATCTCGCCAAATCGCTTACTTTCCCTCTGCCACTTTTAGCAGTAGCTCACCAACAACTTATTCTGGGGTCTTCAGATGGTAACATAGATGATCAAGATGACACATTGATTAAGATCTGGGAAAAGAAACTCGGAGTAAAAATTTCAGATGCATCAAATACTGAAACTTACATCCCTGAGGAATTGGCGAGCCATATTGTTGCTAAATCAGATATGGTGAAGCGGATTGGATTCATTGGTCTTGGAGCAATGGGATTCGGCATGGCAACTCAGCTTCTAAAATCAAACTTTTGTGTTCTTGGTTATGATGTATATAAACCAACCCTAACTCAATTTGCCAATGCTGGTGGCTTGATCGGAAGCTCTCCAGCAGAAGTTTGTAAAGATGTTGATGTTCTGGTAATGATGGTGACAAATGAAACTCAAGCTGAGAGTGCTTTATTTGGAGATTTTGGTGCTGTTTCAGCACTTCCGTCTGGAGCGTCTATTATTCTTTCGTCCACTGTTTCTCCTGGATTTGTGAGCCGACTAGATCAACGCTTTCAAAATGAAGGAAAGAATCTGAAGTTGGTGGATGCTCCTGTTTCTGGTGGTGTTGTGAGAGCCTCCTTGGGGACACTTACGATCATAGCATCTGGCACAGATGAAGCTCTGAAGAGTACTGGTTCAGTCCTCTCAGCATTAAGCGAGAAGCTTTATGTTATAAAAGGGGGCTGCGGGGCAGGAAGTGGCGTAAAGATGGTTAATCAATTGCTTGCTGGGGTTCACATAGCGTCGGGAGCTGAGGCAATGGCATTTGGAGCTCGATTGGGTCTTAATACAAGAATGTTGTTTGATTTCATCACAAATAGTGAGGGATCATCCTGGATGTTTGAAAATCGTGTTCCACACATGCTGGACAATGATTATACACCACTTTCTGCGCTTGATATCTTTGTAAAAGATTTGGGAATTGTTACTCATGAGTCTTCAATTCGAAATGTTCCACTTCATGTTTCGACTATTGCTCATCAACTATTCCTTTCAGGTTCTGCTGCAGGCTGGGGTCGGCAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTCACGGGCGTTAAGGTAGAAGGAAAACTTCCGGCTGTTAAGAAGGACTTTTTATTGCAATCTCTCCCAGCTGAATGGCCGTTAGATCCCATTGGTGAAATACACAAACTAAATCAGGACACATCAAAAACTTTGGTTGTTCTTGATGATGATCCGACTGGAACTCAAACTGTGCATGATATTGAGGTCTTAACTGAATGGACTGTTGAATCACTAATTGAGCAGTTCAGAAAAAGCTCGAAATGCTTTTTCATTTTGACCAACTCCAGGGCACTGAGCTCTGACAAGGCTACCATATTAATAAAAGAAATCTGCACAAACTTGCACACTGCAGCCAAATCAGTACAGTACGCTGATTATACTGTAGTTTTGAGAGGTGATTCGACTTTGCGTGGTCATTTCCCAGAGGAAGCTGATGCTGCTGTTTCAGTATTGGGTGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGCGGGCGTTATACGATCGGAGATATACACTATGTGGCAGATTCTGATGAGCTTATCCCTGCAGCGGATACAGGATTTGCTAAAGATGCTGCCTTTGGCTACAAATCTTCAAATCTTCGCGAGTGGGTTGAGGAGAAAACAGCTGGTCGCATACCAGCCAGCAGTGTTGCTTCCATTTCTATTCAACTGCTGAGAGAAGGTGGACCAGATGCAGTTTGTGAGCATCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAACGCTGCTAGCGAAAGAGATATGGCAGTTTTTGCAGCTGGAATGATCAAGGCAGAACTTAAAGGCAAGCAATTCTTATGCCGCACCGCTGCTAGTTTTGTTTCTGCAAGGATTGGAATTATTCCCAAAGCTCCAATCTTGCCTAGGGATCTAGGAATAAACAAGGAACATAATGGTGGTCTAATAGTTGTTGGATCATATGTTGCAAAGACAACAAGACAGGTTGAAGAGCTTAAGTTACAATGCGGTCAGATTCTGAGAAACATTGAGGTTTCGGTTGCTAAAGTTGCCATGAGGTCTGCAGAAGAGAGGGAGGAGGAAATCAGCACAGCAGCTGAGATGGCAGATATTTTTCTTGCAGCTCAGAACGACACTTTAATAGTAACCAGTCGGGAGCTTATTACGGGCAAAAGTCCCTCTGAGAGTTTGGAAATCAACTTCAAAGTAAGCTCTGCACTGGTGGAAATAGTTAGGCGAATAACAAAAAGACCTCGTTACATCCTTGCAAAGGGTGGAATCACATCTTCTGACCTTGCTACGAAAGCCCTTGAAGCAAAATGTGCCAAAATAGTGGGACAAGCCTTGCCTGGTGTTCCCTTATGGCAGTTGGGTCCTGAAAGTAGGCACCTGGGAGTTCCATACATTGTTTTTCCTGGTAACGTTGGCGACAGTGGAGCATTGGCGGAGCTAGTGAAATCTTGGGCTCGTCCAGTCAAATTTTCGACGAAAGAGCTTCTTCTGAACGCAGAAAAGGGTGGATATGCTGTTGGAGCATTCAATGTTTATAACTTAGAAGGAGTAGAGGCAGTTGTTTCTGCAGCAGAGGAACAACAGAGTCCTGCCATATTACAGATCCATCCCGGTGCCTTGAAGCAAGGAGGCAGGCCATTGATTGCTTGTTGTATCTCTGCTGCTGAACAAGCCAGCGTACCCATAACTGTACACTTTGATCATGGAACTTCGAAGCAAGATCTGGTGACAGCTCTTGAGTTGGGATTTAATTCTGTCATGGTAGATGGTTCACATCTTTCCTTTAGAGAAAATGTTTCGTACACAAAGTTCATATCACTCTTAGCTCACTCAAAAGGCTTGCTAGTAGAAGCTGAACTAGGGAGATTGTCAGGGACGGAAGATGATTTGACTGTTGAAGATTATGAAGCAAGGCTAACCGATGTTAAGCAGGCTCAAGAGTTCATCGATGAAACCGGTATAGATGCTTTGGCAGTTTGTATTGGAAATGTCCATGGTAAATACCCCGCAAGTGGCCCAAATCTGAGACTTGATTTGCTCAAGGATTTGCATGCTTTGAGTTCAAAGAAAGGAGTCTTCCTGGTGCTTCATGGAGCTTCTGGTGTGCCCGAGGAACTTGTAAAGAGATGCATAGAGCTAGGTGTGAGAAAGTTCAATGTAAATACTGAGGTACGGAAAGCTTACATGGATTCCCTGAACAACCCCAAGAAAGACCTGGTTCATGTAATGGAGTCGGCAAAACAAGCCATGAAAGCAGTGATTGCAGAGAAGATGATTCTATTTGGCTCAGCAGGAAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1372

Amino Acids

147.36

Weight (kDa)

5.79

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 7 - 157 7.5e-06 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 164 - 284 1.7e-17 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 321 - 480 4.3e-34 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 486 - 606 2e-28 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 653 - 890 1.9e-57 Sugar-binding N-terminal domain
NBD_C PF17042 915 - 1081 1.1e-33 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1095 - 1370 9.4e-93 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 327, 1442, 3348
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 3 cut(s) 572, 3186, 3425
AccII CGCG 1 cut(s) 2421
AclI AACGTT 2 cut(s) 275, 3222
AclWI GGATC 9 cut(s) 747, 1603, 1904, 1970, 2285, 2554, 2718, 2764, 3409
AcoI YGGCCR 2 cut(s) 624, 1901
AcsI RAATTY 3 cut(s) 298, 879, 3278
AcuI CTGAAG 5 cut(s) 390, 711, 795, 1322, 1412
AcyI GRCGYC 1 cut(s) 420
AdeI CACNNNGTG 1 cut(s) 2335
AfaI GTAC 9 cut(s) 290, 574, 1399, 2169, 2174, 3492, 3504, 3614, 3984
AfiI CCNNNNNNNGG 9 cut(s) 49, 166, 680, 1325, 1350, 2091, 2365, 3070, 3824
AflII CTTAAG 1 cut(s) 2795
AflIII ACRYGT 1 cut(s) 308
AgeI ACCGGT 1 cut(s) 3767
AjiI CACGTC 1 cut(s) 309
AjnI CCWGG 8 cut(s) 1249, 1601, 2090, 3148, 3189, 3214, 3891, 4026
AjuI GAANNNNNNNTTGG 4 cut(s) 783, 815, 1289, 1321
Alw21I GWGCWC 3 cut(s) 688, 1543, 2105
Alw26I GTCTC 3 cut(s) 95, 1814, 3832
AlwI GGATC 9 cut(s) 747, 1603, 1904, 1970, 2285, 2554, 2718, 2764, 3409
AlwNI CAGNNNCTG 5 cut(s) 371, 1769, 2345, 2818, 2897
Ama87I CYCGRG 1 cut(s) 3920
AoxI GGCC 4 cut(s) 624, 1901, 3446, 3826
ApoI RAATTY 3 cut(s) 298, 879, 3278
AseI ATTAAT 2 cut(s) 222, 2123
AsiGI ACCGGT 1 cut(s) 3767
Asp718I GGTACC 1 cut(s) 572
AspA2I CCTAGG 1 cut(s) 2705
AspLEI GCGC 1 cut(s) 1663
AspS9I GGNCC 4 cut(s) 144, 2504, 3173, 3827
AsuC2I CCSGG 1 cut(s) 3423
AsuHPI GGTGA 8 cut(s) 494, 779, 967, 1159, 1931, 2213, 2273, 3550
AsuII TTCGAA 2 cut(s) 1714, 3524
AsuNHI GCTAGC 1 cut(s) 2570
AvaI CYCGRG 1 cut(s) 3920
AvaII GGWCC 3 cut(s) 144, 2504, 3173
AvrII CCTAGG 1 cut(s) 2705
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 2 cut(s) 2098, 3921
BanI GGYRCC 3 cut(s) 572, 3186, 3425
BanII GRGCYC 3 cut(s) 1543, 2105, 3268
BarI GAAGNNNNNNTAC 2 cut(s) 684, 716
BbsI GAAGAC 3 cut(s) 801, 1698, 3880
Bbv12I GWGCWC 3 cut(s) 688, 1543, 2105
BbvCI CCTCAGC 3 cut(s) 44, 660, 1521
BccI CCATC 8 cut(s) 505, 809, 1138, 1471, 2260, 2896, 3426, 3572
BceAI ACGGC 2 cut(s) 63, 1888
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 8 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3893, 4028
BciVI GTATCC 2 cut(s) 244, 2362
BclI TGATCA 3 cut(s) 829, 2607, 3511
BcnI CCSGG 1 cut(s) 3423
BcoDI GTCTC 3 cut(s) 95, 1814, 3832
BfmI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
BfrI CTTAAG 1 cut(s) 2795
BfuI GTATCC 2 cut(s) 244, 2362
BglI GCCNNNNNGGC 1 cut(s) 362
BglII AGATCT 2 cut(s) 852, 3532
BlnI CCTAGG 1 cut(s) 2705
BlpI GCTNAGC 1 cut(s) 4099
BmcAI AGTACT 1 cut(s) 1399
Bme1390I CCNGG 9 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3423, 3893, 4028
Bme18I GGWCC 3 cut(s) 144, 2504, 3173
BmeT110I CYCGRG 1 cut(s) 3920
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 4 cut(s) 144, 2504, 3173, 3827
BmiI GGNNCC 5 cut(s) 145, 574, 3174, 3188, 3427
BmrFI CCNGG 9 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3423, 3893, 4028
BmtI GCTAGC 1 cut(s) 2574
BpiI GAAGAC 3 cut(s) 801, 1698, 3880
BpmI CTGGAG 3 cut(s) 1089, 1236, 2074
Bpu10I CCTNAGC 3 cut(s) 44, 660, 1521
Bpu1102I GCTNAGC 1 cut(s) 4099
Bpu14I TTCGAA 2 cut(s) 1714, 3524
BpuEI CTTGAG 5 cut(s) 548, 1146, 3569, 3732, 3836
BpuMI CCSGG 1 cut(s) 3423
Bsa29I ATCGAT 1 cut(s) 3759
BsaBI GATNNNNATC 2 cut(s) 851, 1974
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 7 cut(s) 593, 1347, 2091, 2705, 3190, 3805, 3921
BsaWI WCCGGW 1 cut(s) 3767
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 9 cut(s) 49, 166, 680, 1325, 1350, 2091, 2365, 3070, 3824
Bse118I RCCGGY 1 cut(s) 3767
Bse1I ACTGG 6 cut(s) 440, 1405, 1987, 2949, 3024, 3272
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 3 cut(s) 459, 993, 1533
Bse8I GATNNNNATC 2 cut(s) 851, 1974
BseBI CCWGG 8 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3893, 4028
BseCI ATCGAT 1 cut(s) 3759
BseDI CCNNGG 7 cut(s) 593, 1347, 2091, 2705, 3190, 3805, 3921
BseGI GGATG 9 cut(s) 43, 589, 909, 1318, 1598, 1611, 2275, 3060, 3418
BseJI GATNNNNATC 2 cut(s) 851, 1974
BseLI CCNNNNNNNGG 9 cut(s) 49, 166, 680, 1325, 1350, 2091, 2365, 3070, 3824
BseMI GCAATG 3 cut(s) 459, 993, 1533
BseNI ACTGG 6 cut(s) 440, 1405, 1987, 2949, 3024, 3272
BseRI GAGGAG 2 cut(s) 2447, 2891
BseSI GKGCMC 2 cut(s) 2098, 3921
BseX3I CGGCCG 1 cut(s) 624
BseYI CCCAGC 3 cut(s) 1496, 1779, 1891
BsgI GTGCAG 2 cut(s) 2122, 3000
Bsh1236I CGCG 1 cut(s) 2421
Bsh1285I CGRYCG 2 cut(s) 627, 2319
BshFI GGCC 4 cut(s) 626, 1903, 3448, 3828
BshNI GGYRCC 3 cut(s) 572, 3186, 3425
BshTI ACCGGT 1 cut(s) 3767
BshVI ATCGAT 1 cut(s) 3759
BsiEI CGRYCG 2 cut(s) 627, 2319
BsiHKAI GWGCWC 3 cut(s) 688, 1543, 2105
BsiHKCI CYCGRG 1 cut(s) 3920
BsiSI CCGG 4 cut(s) 627, 1856, 3423, 3768
BslFI GGGAC 5 cut(s) 157, 1366, 2961, 3150, 3700
BslI CCNNNNNNNGG 9 cut(s) 49, 166, 680, 1325, 1350, 2091, 2365, 3070, 3824
BsmAI GTCTC 3 cut(s) 95, 1814, 3832
BsmFI GGGAC 5 cut(s) 157, 1366, 2961, 3150, 3700
BsmI GAATGC 2 cut(s) 246, 3335
BsnI GGCC 4 cut(s) 626, 1903, 3448, 3828
BsoBI CYCGRG 1 cut(s) 3920
Bsp119I TTCGAA 2 cut(s) 1714, 3524
Bsp1286I GDGCHC 6 cut(s) 688, 1543, 2098, 2105, 3268, 3921
Bsp1407I TGTACA 2 cut(s) 288, 3502
Bsp1720I GCTNAGC 1 cut(s) 4099
Bsp19I CCATGG 1 cut(s) 3805
Bsp68I TCGCGA 1 cut(s) 2421
BspANI GGCC 4 cut(s) 626, 1903, 3448, 3828
BspDI ATCGAT 1 cut(s) 3759
BspFNI CGCG 1 cut(s) 2421
BspHI TCATGA 2 cut(s) 393, 1699
BspLI GGNNCC 5 cut(s) 145, 574, 3174, 3188, 3427
BspMAI CTGCAG 5 cut(s) 1777, 2158, 2365, 2866, 3382
BspOI GCTAGC 1 cut(s) 2574
BspPI GGATC 9 cut(s) 747, 1603, 1904, 1970, 2285, 2554, 2718, 2764, 3409
BspQI GCTCTTC 1 cut(s) 2784
BspT104I TTCGAA 2 cut(s) 1714, 3524
BspT107I GGYRCC 3 cut(s) 572, 3186, 3425
BspTI CTTAAG 1 cut(s) 2795
BsrDI GCAATG 3 cut(s) 459, 993, 1533
BsrFI RCCGGY 1 cut(s) 3767
BsrGI TGTACA 2 cut(s) 288, 3502
BsrI ACTGG 6 cut(s) 440, 1405, 1987, 2949, 3024, 3272
BssAI RCCGGY 1 cut(s) 3767
BssECI CCNNGG 7 cut(s) 593, 1347, 2091, 2705, 3190, 3805, 3921
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 3 cut(s) 1347, 2705, 3805
Bst2UI CCWGG 8 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3893, 4028
Bst4CI ACNGT 9 cut(s) 256, 1243, 1996, 2029, 2172, 2188, 3233, 3502, 3706
Bst6I CTCTTC 3 cut(s) 1388, 2784, 2862
BstACI GRCGYC 1 cut(s) 420
BstAFI CTTAAG 1 cut(s) 2795
BstAPI GCANNNNNTGC 3 cut(s) 2594, 3122, 4073
BstAUI TGTACA 2 cut(s) 288, 3502
BstBI TTCGAA 2 cut(s) 1714, 3524
BstDSI CCRYGG 2 cut(s) 593, 3805
BstENI CCTNNNNNAGG 1 cut(s) 3068
BstF5I GGATG 9 cut(s) 43, 589, 909, 1318, 1598, 1611, 2275, 3060, 3418
BstFNI CGCG 1 cut(s) 2421
BstHHI GCGC 1 cut(s) 1663
BstMAI GTCTC 3 cut(s) 95, 1814, 3832
BstMCI CGRYCG 2 cut(s) 627, 2319
BstNI CCWGG 8 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3893, 4028
BstNSI RCATGY 3 cut(s) 1633, 2556, 3866
BstSCI CCNGG 9 cut(s) 1249, 1601, 2090, 3148, 3189, 3214, 3421, 3891, 4026
BstSFI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
BstSLI GKGCMC 2 cut(s) 2098, 3921
BstUI CGCG 1 cut(s) 2421
BstV2I GAAGAC 3 cut(s) 801, 1698, 3880
BstX2I RGATCY 6 cut(s) 739, 852, 1909, 2710, 3414, 3532
BstXI CCANNNNNNTGG 2 cut(s) 1899, 3133
BstYI RGATCY 6 cut(s) 739, 852, 1909, 2710, 3414, 3532
BstZI CGGCCG 1 cut(s) 624
Bsu15I ATCGAT 1 cut(s) 3759
Bsu36I CCTNAGG 1 cut(s) 915
BsuI GTATCC 2 cut(s) 244, 2362
BsuRI GGCC 4 cut(s) 626, 1903, 3448, 3828
BsuTUI ATCGAT 1 cut(s) 3759
BtgI CCRYGG 2 cut(s) 593, 3805
BtrI CACGTC 1 cut(s) 309
BtsCI GGATG 9 cut(s) 43, 589, 909, 1318, 1598, 1611, 2275, 3060, 3418
BtsI GCAGTG 3 cut(s) 2151, 2470, 4074
BtsIMutI CAGTG 7 cut(s) 1239, 2096, 2151, 2470, 3017, 3238, 4074
BtuMI TCGCGA 1 cut(s) 2421
CaiI CAGNNNCTG 5 cut(s) 371, 1769, 2345, 2818, 2897
CciI TCATGA 2 cut(s) 393, 1699
CfoI GCGC 1 cut(s) 1663
Cfr10I RCCGGY 1 cut(s) 3767
Cfr13I GGNCC 4 cut(s) 144, 2504, 3173, 3827
ClaI ATCGAT 1 cut(s) 3759
CseI GACGC 4 cut(s) 236, 428, 1209, 1500
CsiI ACCWGGT 1 cut(s) 4026
Csp6I GTAC 9 cut(s) 289, 573, 1398, 2168, 2173, 3491, 3503, 3613, 3983
CspAI ACCGGT 1 cut(s) 3767
CspCI CAANNNNNGTGG 2 cut(s) 1066, 1101
CviQI GTAC 9 cut(s) 289, 573, 1398, 2168, 2173, 3491, 3503, 3613, 3983
DraI TTTAAA 2 cut(s) 381, 429
DraIII CACNNNGTG 1 cut(s) 2335
EaeI YGGCCR 2 cut(s) 624, 1901
EagI CGGCCG 1 cut(s) 624
Eam1104I CTCTTC 3 cut(s) 1388, 2784, 2862
EarI CTCTTC 3 cut(s) 1388, 2784, 2862
EciI GGCGGA 1 cut(s) 3260
Ecl136II GAGCTC 2 cut(s) 1541, 2103
EclXI CGGCCG 1 cut(s) 624
Eco130I CCWWGG 3 cut(s) 1347, 2705, 3805
Eco24I GRGCYC 3 cut(s) 1543, 2105, 3268
Eco32I GATATC 2 cut(s) 610, 1669
Eco47I GGWCC 3 cut(s) 144, 2504, 3173
Eco52I CGGCCG 1 cut(s) 624
Eco53kI GAGCTC 2 cut(s) 1541, 2103
Eco57I CTGAAG 5 cut(s) 390, 711, 795, 1322, 1412
Eco81I CCTNAGG 1 cut(s) 915
Eco88I CYCGRG 1 cut(s) 3920
EcoICRI GAGCTC 2 cut(s) 1541, 2103
EcoNI CCTNNNNNAGG 1 cut(s) 3068
EcoO109I RGGNCCY 1 cut(s) 3173
EcoRII CCWGG 8 cut(s) 1249, 1601, 2090, 3148, 3189, 3214, 3891, 4026
EcoRV GATATC 2 cut(s) 610, 1669
EcoT14I CCWWGG 3 cut(s) 1347, 2705, 3805
EcoT22I ATGCAT 4 cut(s) 892, 2275, 2558, 3944
EcoT38I GRGCYC 3 cut(s) 1543, 2105, 3268
ErhI CCWWGG 3 cut(s) 1347, 2705, 3805
FalI AAGNNNNNCTT 8 cut(s) 1836, 1868, 1856, 1888, 2621, 2653, 3282, 3314
FaqI GGGAC 5 cut(s) 157, 1366, 2961, 3150, 3700
FauI CCCGC 3 cut(s) 1448, 2297, 3826
FauNDI CATATG 3 cut(s) 106, 654, 2761
FbaI TGATCA 3 cut(s) 829, 2607, 3511
FokI GGATG 9 cut(s) 50, 576, 896, 1325, 1585, 1618, 2282, 3047, 3405
FriOI GRGCYC 3 cut(s) 1543, 2105, 3268
GlaI GCGC 1 cut(s) 1662
GsaI CCCAGC 3 cut(s) 1500, 1783, 1895
GsuI CTGGAG 3 cut(s) 1089, 1236, 2074
HaeIII GGCC 4 cut(s) 626, 1903, 3448, 3828
HapII CCGG 4 cut(s) 627, 1856, 3423, 3768
HgaI GACGC 4 cut(s) 236, 428, 1209, 1500
HhaI GCGC 1 cut(s) 1663
Hin1I GRCGYC 1 cut(s) 420
Hin6I GCGC 1 cut(s) 1661
HinP1I GCGC 1 cut(s) 1661
HincII GTYRAC 2 cut(s) 201, 2563
HindII GTYRAC 2 cut(s) 201, 2563
HindIII AAGCTT 4 cut(s) 93, 464, 1430, 3990
HpaI GTTAAC 2 cut(s) 201, 2563
HpaII CCGG 4 cut(s) 627, 1856, 3423, 3768
HphI GGTGA 8 cut(s) 494, 779, 967, 1159, 1931, 2213, 2273, 3550
Hpy166II GTNNAC 8 cut(s) 201, 1239, 1504, 2504, 2563, 3505, 3584, 3615
Hpy8I GTNNAC 8 cut(s) 201, 1239, 1504, 2504, 2563, 3505, 3584, 3615
Hpy99I CGWCG 2 cut(s) 1516, 3289
HpyAV CCTTC 8 cut(s) 241, 355, 1283, 1838, 1861, 2492, 2536, 3350
HpyCH4III ACNGT 9 cut(s) 256, 1243, 1996, 2029, 2172, 2188, 3233, 3502, 3706
HpyCH4IV ACGT 3 cut(s) 275, 308, 3222
HpySE526I ACGT 3 cut(s) 275, 308, 3222
Hsp92I GRCGYC 1 cut(s) 420
HspAI GCGC 1 cut(s) 1661
KpnI GGTACC 1 cut(s) 576
Ksp22I TGATCA 3 cut(s) 829, 2607, 3511
KspAI GTTAAC 2 cut(s) 201, 2563
LguI GCTCTTC 1 cut(s) 2784
MabI ACCWGGT 1 cut(s) 4026
MaeII ACGT 3 cut(s) 275, 308, 3222
MfeI CAATTG 1 cut(s) 1487
MflI RGATCY 6 cut(s) 739, 852, 1909, 2710, 3414, 3532
MhlI GDGCHC 6 cut(s) 688, 1543, 2098, 2105, 3268, 3921
MlyI GAGTC 4 cut(s) 1712, 3406, 3894, 4052
MmeI TCCRAC 3 cut(s) 2003, 2734, 3310
Mph1103I ATGCAT 4 cut(s) 892, 2275, 2558, 3944
MslI CAYNNNNRTG 2 cut(s) 2736, 3423
MspA1I CMGCKG 8 cut(s) 75, 320, 1895, 2366, 2444, 2600, 2651, 2897
MspCI CTTAAG 1 cut(s) 2795
MspI CCGG 4 cut(s) 627, 1856, 3423, 3768
MspR9I CCNGG 9 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3423, 3893, 4028
MunI CAATTG 1 cut(s) 1487
Mva1269I GAATGC 2 cut(s) 246, 3335
MvaI CCWGG 8 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3893, 4028
MvnI CGCG 1 cut(s) 2421
NciI CCSGG 1 cut(s) 3423
NcoI CCATGG 1 cut(s) 3805
NdeI CATATG 3 cut(s) 106, 654, 2761
NheI GCTAGC 1 cut(s) 2570
NlaIV GGNNCC 5 cut(s) 145, 574, 3174, 3188, 3427
NmuCI GTSAC 2 cut(s) 1147, 3538
NruI TCGCGA 1 cut(s) 2421
NsiI ATGCAT 4 cut(s) 892, 2275, 2558, 3944
NspI RCATGY 3 cut(s) 1633, 2556, 3866
NspV TTCGAA 2 cut(s) 1714, 3524
PaeI GCATGC 1 cut(s) 3866
PagI TCATGA 2 cut(s) 393, 1699
PciSI GCTCTTC 1 cut(s) 2784
PcsI WCGNNNNNNNCGW 1 cut(s) 2571
PctI GAATGC 2 cut(s) 246, 3335
PfoI TCCNGGA 2 cut(s) 1249, 1601
PinAI ACCGGT 1 cut(s) 3767
Ple19I CGATCG 1 cut(s) 2319
PleI GAGTC 4 cut(s) 1711, 3405, 3893, 4051
PpsI GAGTC 4 cut(s) 1711, 3405, 3893, 4051
PpuMI RGGWCCY 1 cut(s) 3173
PshBI ATTAAT 2 cut(s) 222, 2123
PsiI TTATAA 3 cut(s) 327, 1442, 3348
Psp124BI GAGCTC 2 cut(s) 1543, 2105
Psp1406I AACGTT 2 cut(s) 275, 3222
Psp5II RGGWCCY 1 cut(s) 3173
Psp6I CCWGG 8 cut(s) 1249, 1601, 2090, 3148, 3189, 3214, 3891, 4026
PspFI CCCAGC 3 cut(s) 1496, 1779, 1891
PspGI CCWGG 8 cut(s) 1249, 1601, 2090, 3148, 3189, 3214, 3891, 4026
PspN4I GGNNCC 5 cut(s) 145, 574, 3174, 3188, 3427
PspPI GGNCC 4 cut(s) 144, 2504, 3173, 3827
PspPPI RGGWCCY 1 cut(s) 3173
PsrI GAACNNNNNNTAC 2 cut(s) 3605, 3637
PstI CTGCAG 5 cut(s) 1777, 2158, 2365, 2866, 3382
PstNI CAGNNNCTG 5 cut(s) 371, 1769, 2345, 2818, 2897
PsuI RGATCY 6 cut(s) 739, 852, 1909, 2710, 3414, 3532
PvuI CGATCG 1 cut(s) 2319
PvuII CAGCTG 5 cut(s) 320, 1895, 2444, 2600, 2897
RruI TCGCGA 1 cut(s) 2421
RsaI GTAC 9 cut(s) 290, 574, 1399, 2169, 2174, 3492, 3504, 3614, 3984
RsaNI GTAC 9 cut(s) 289, 573, 1398, 2168, 2173, 3491, 3503, 3613, 3983
RseI CAYNNNNRTG 2 cut(s) 2736, 3423
SacI GAGCTC 2 cut(s) 1543, 2105
SapI GCTCTTC 1 cut(s) 2784
Sau96I GGNCC 4 cut(s) 144, 2504, 3173, 3827
ScaI AGTACT 1 cut(s) 1399
SchI GAGTC 4 cut(s) 1712, 3406, 3894, 4052
ScrFI CCNGG 9 cut(s) 1251, 1603, 2092, 3150, 3191, 3216, 3423, 3893, 4028
SduI GDGCHC 6 cut(s) 688, 1543, 2098, 2105, 3268, 3921
SexAI ACCWGGT 1 cut(s) 4026
SfcI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
SfuI TTCGAA 2 cut(s) 1714, 3524
SinI GGWCC 3 cut(s) 144, 2504, 3173
SmiMI CAYNNNNRTG 2 cut(s) 2736, 3423
SmlI CTYRAG 6 cut(s) 527, 1161, 2795, 3548, 3747, 3851
SmoI CTYRAG 6 cut(s) 527, 1161, 2795, 3548, 3747, 3851
SphI GCATGC 1 cut(s) 3866
SstI GAGCTC 2 cut(s) 1543, 2105
StyD4I CCNGG 9 cut(s) 1249, 1601, 2090, 3148, 3189, 3214, 3421, 3891, 4026
StyI CCWWGG 3 cut(s) 1347, 2705, 3805
TaaI ACNGT 9 cut(s) 256, 1243, 1996, 2029, 2172, 2188, 3233, 3502, 3706
TaiI ACGT 3 cut(s) 278, 311, 3225
TaqI TCGA 9 cut(s) 10, 1543, 1714, 1736, 2063, 2207, 3284, 3524, 3759
TatI WGTACW 4 cut(s) 288, 1397, 2167, 3502
TauI GCSGC 2 cut(s) 626, 2646
TscAI CASTG 7 cut(s) 1246, 2103, 2158, 2470, 3024, 3238, 4074
TseFI GTSAC 2 cut(s) 1147, 3538
Tsp45I GTSAC 2 cut(s) 1147, 3538
TspGWI ACGGA 4 cut(s) 582, 1200, 3704, 4000
TspRI CASTG 7 cut(s) 1246, 2103, 2158, 2470, 3024, 3238, 4074
Vha464I CTTAAG 1 cut(s) 2795
VpaK11BI GGWCC 3 cut(s) 144, 2504, 3173
VspI ATTAAT 2 cut(s) 222, 2123
XagI CCTNNNNNAGG 1 cut(s) 3068
XapI RAATTY 3 cut(s) 298, 879, 3278
XceI RCATGY 3 cut(s) 1633, 2556, 3866
XcmI CCANNNNNNNNNTGG 1 cut(s) 4024
XmaJI CCTAGG 1 cut(s) 2705
ZrmI AGTACT 1 cut(s) 1399
Zsp2I ATGCAT 4 cut(s) 892, 2275, 2558, 3944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.