MD14G1237000.v1.1

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006366
Physical Location & Seq
Reverse (-)
31673817 .. 31685182
11366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1237000.v1.1.491

Sequence Viewer

Length: 4110 bp
ATGGCGTCCAAGGGAGCTGTTCGGTTCGTCGGCTTGGATGCCTTGAGCTTGGACTTGGCTTCCTCTCTCCTCCGCAGTGGCTACAAAGTCCAAGCTTTCGAGATGGATGGGAATTTGATTAGTGAGTTTTTGAAGCTTGGAGGAACCAGATGTGGCGGCCCAGAGGAGGCAGGAAAAGATGTGGCAGCTTTGGTTGTATTAGCAGATCAAGTAAATGATGCAACTTTCAGTGATGAGGGTGCATCGAAAGGATTTCAGAAGGATACTGTTGTCATACTTCGTTCAACAATATTACCATCGTCTATTCAGAATCTAAAGACGCGTTTCACAGATACAGCTGATCTTGTAGATATATATGCCACAAAGGGAGTATCTGATGGTTTAAATGGAAAGATCATTATTGCGTCTTCCGGAGGTTCAGAAACCATTTTAAAATCACGGCCAGTTCTCTCTGCAATGTGCGATAAGCTTTATGTTTTTGAGGGTGAAGTTGGCGCTGGAAGCAAAATTAGGATGATTAAGGAACTGCTTGAGGGTATTCATCTTGTTGCTTCTTTGGAGGCTATTTCTCTTGGCACCAAAGCTGGTATTCATCCGTGGATAATCTACGATATCATATCTAATGCTGCGGGAAACTCATGGGTTTTCAAGAACCATATTCCTCAGTTTTTAAGGGGTGCTGCCAAAGATGATTTGAATACTCTTGTTCAAAACTTGAGGGTTATATTGGATCTGGCCAAGTCGCTTAGCTTTCCTCTCCCGCTTTTAGCAGTTGCTCATCAGCAACTTATTCTTGGGTCTCCAAGTGATAACACAGATGATGAAGATGCTACATTGATTAAGGTTTGGGAAAAGAAACTTGGAGTGAGAATTTTAGATGCAGCAAATGCTGAGACTTACATCCCTGAGGAATTGGCAAGCCACACTGTTGCTAAATCATATACCGTCAACCGAGTTGGATTCATTGGTCTTGGAGCAATGGGATTTGGAATGGCAACTCACCTTTTAAAAGCTAACTTTTCTGTTTGTGGTTATGATGTATATAAACCAACACTGACTCGTTTTGCCAATGCTGGTGGCTCGATTGGAAGCTCTCCAGCAGAAGTATGTAAAGATGTTGATGTGCTGGTAATAATGGTCACAAATGAAGCTCAAGCTGAGAGTGCTTTGTATGGAGACTTTGGTGCTATTTCAGCACTTCCGTCTGGAGCTTCTATTATCCTTTCATCCACTGTTTCTCCTGGATTTGTGAGCCGACTAGAACACCGCTTGCAAAATGAAGGAAAGAATTTCAAGTTGGTGGATGCTCCTGTTTCTGGTGGGGTTGTGAGAGCCTCCATGGGGGCACTTACGATTATGGCATCCGGTACAGATGAAGCTCTTAAGAGTGCTGGTGCAGTCCTCTCAGCATTAAGCGAGAAGCTTTATGTTATCAAAGGAGGCTGTGGGGCTGGAAGTGGTGTAAAGATGGTTAATCAATTGCTTGCTGGAGTTCATATAGCGTCGGGAGCTGAAGCAATGGCTTTTGGAGCTCGTTTGGGTCTTAATACAAGGATGTTGTTTGATTTTATCACAAATAGTGAGGGAACATCCTGGATGTTTGAAAATCGTGTTCCACACATGCTCGACAATGATTATACTCTGCATTCTGCACTTGATATTTTTGTGAAGGATCTGGGAATTGTTTCTCAAGATTGTTCAGTTCGAAAAATTCCACTTCATATATCGACTGTTGCTCATCAACTCTTCCTGTCAGGTTCTGCTGCAGGTTGGGGTCGGCAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTAACGGGTGTTAAGGTAGAAGCAAAACTTCCGGTTCTTAAGAAGGACGTTGTCTTGAAGTCTCTCCCAGTTGAGTGGCCAGTAGATCCCACTGGTGAAATACAGAGACTAAATCAGGACAGTTCAAAGACTTTGGTTGTTCTTGATGACGATCCAACTGGAACTCAAACTGTTCATGATATTGAGGTATTGACGGAATGGACCGTTGAATCACTAACGGAGCAGTTCAGAAAAGGTCCTAAATGCTTTTTCATTTTGACCAACTCCAGGGCCTTGAGTTCTGAGAAGGCTACTGCATTAATTAAAGACATCTGCACAAACCTGAGTACTGCAGCCAAATCAGTTGAGAATGCTGATTATACAGTAGTTTTGAGAGGCGATTCAACTTTGCGTGGCCATTTCCCAGAGGAAGCTGATGCCGCTGTTTCAGTACTGGGCGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGAGGGCGTTATACTGTTGGAGATATACACTATGTTGCAGATTCGGATCAGCTTATCCCTGCAGGGGATACAGGATTCGCTAAAGATGCTGCATTTGGCTACAAATCTTCAAACCTTCGCGAGTGGGTTGAGGAGAAAACAGCAGGCCGCATACCAGCCAGTAGTGTTACATCTATTTCTATCCAAATTCTAAGAAAAGGTGGGCCAGATGCCGTTTGTGAGCGTCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAACGCAGCTAGTGACAGGGACATGGCTGTTTTTGCAGCTGGAATGATCAAGGCGGAACTGAAAGGCAAGCGCTTCTTATGTCGCACTGCTGCTAGCTTTGTTTCTGCCAGGATTGGAATTATCCCCAAAGCTCCAATTCTGCCCAAGGATCTAGGAATAAACAAGGAAAATAACGGAGGTCTCATAGTTGTCGGGTCATATGTTCCCAAAACGACAAAACAGGTGGAAGAGCTTAAACTACAGTGCAGCCAGATTTTGAGAAGCATTGAGGTTTCGGTTGCTAAAGTAGCCATGAGTTCTACAGAAGAGAGGGAGGAGGAAATCAGTAGAGCAGCTGAGATGGCAGATATTTTCCTTACAGCTCGGAAGGACACTCTAATAATGACCAGTCGGGAGCTTATTACCGGAAAAACTCCCTCTGAGAGTTTGGAAATCAATTTCAAAGTTAGCTCGGCACTGGTGGAAATAGTTAGACGCATAACTACAAGACCACGTTACATTCTTGCAAAGGGTGGAATCACTTCATCTGACCTTGCTACGAAAGCTCTTGAAGCAAAATGTGCAAAAATAGTGGGACAAGCCCTGGCTGGTGTTCCCTTATGGCAGCTAGGTCCTGAGAGTAGGCATCTGGGAGTTCCATACATTGTTTTTCCTGGTAATGTTGGTGACGAAAGAGCGTTGGCGGAGGTCGTGAAGTCCTGGGCTCGTCCAGTCAGACTTTCATCAACCAAAGAGCTTCTTCTGAATGCAGAAAAGGGTGGATATGCCGTAGGAGCATTCAATGTATATAATTTGGAAGGAGTAGAGGCAGTTGTTGCTGCAGCAGAGGAAGAACAAAGTCCGGCTATATTACAGATCCATCCAGGTGCCTTGAAGCAAGGAGGGATCCCGTTGGTTGCATGTTGTATCGCTGCTGCAGAACAAGCCAGTGTTCCCATAACTGTACACTTTGACCACGGAACTTCAAAGCAAGATCTGGTGGAAGCTCTTGAGTTGGGATTTGATTCTGTCATGGCGGATGGTTCACATCTATCATTTACCGAGAATGTTTCATACACCAAGTTTGTCGCATTCTTTGCTCACTCAAAAGGCGTGCTAGTAGAAGCGGAACTGGGGAGATTGTCGGGGACAGAAGATGATTTAACCGTCGAAGATTATGAAGCAAGGCTGACTGATGTTAAGCAGGCTGAGGAGTTCATTGATAAAACCGGGATAGATGCTTTGGCAGTCTGTATTGGAAATGTGCACGGAAAGTACCCTGCAAGTGGCCCGAATCTGAGACTCGATCTGCTTAAGGATCTGTATGCTTTGAGTTCGAAGAAAGGAGTCCTCCTGGTGCTTCATGGCGCTTCTGGTTTGTCGAAGGGACTTATAAAGGAATGCATAGAGCATGGTGTGAGGAAGTTCAATGTAAACACCGAGGTGCGAAAAGCTTACACGGATTCCCTTAGCAACTCGAAGAAAGACCTGGTCCACGTTATGGCGTCGGCGAAGGAAGCCATGAAAGCAGTGGTTGCAGAGAAGATGCATCTATTTGGTTCAGCAGGAAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1370

Amino Acids

146.19

Weight (kDa)

5.86

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 8 - 158 3.4e-09 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 165 - 282 3.4e-17 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 318 - 477 1.8e-36 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 483 - 603 3.8e-27 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 650 - 887 3e-58 Sugar-binding N-terminal domain
NBD_C PF17042 912 - 1078 2.8e-35 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1093 - 1368 3.4e-93 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3926
Acc36I ACCTGC 1 cut(s) 1757
AccB1I GGYRCC 2 cut(s) 575, 3419
AccB7I CCANNNNNTGG 1 cut(s) 4033
AccII CGCG 2 cut(s) 322, 2412
AccIII TCCGGA 1 cut(s) 410
AcoI YGGCCR 4 cut(s) 440, 735, 1892, 2209
AcsI RAATTY 5 cut(s) 112, 870, 1288, 1710, 2478
AcuI CTGAAG 1 cut(s) 1533
AcyI GRCGYC 2 cut(s) 5, 4037
AfaI GTAC 5 cut(s) 1369, 2143, 2247, 3498, 3809
AfeI AGCGCT 1 cut(s) 2624
AfiI CCNNNNNNNGG 7 cut(s) 166, 1316, 1341, 2082, 2356, 3818, 4033
AflII CTTAAG 3 cut(s) 1382, 1853, 3845
AflIII ACRYGT 1 cut(s) 320
AjuI GAANNNNNNNTTGG 4 cut(s) 843, 875, 1280, 1312
AleI CACNNNNGTG 1 cut(s) 3974
Alw21I GWGCWC 2 cut(s) 1534, 3801
Alw26I GTCTC 8 cut(s) 804, 887, 1170, 1805, 1881, 1915, 2738, 3826
Alw44I GTGCAC 1 cut(s) 3797
AlwNI CAGNNNCTG 1 cut(s) 1760
Aor13HI TCCGGA 1 cut(s) 410
Aor51HI AGCGCT 1 cut(s) 2624
AoxI GGCC 9 cut(s) 157, 440, 735, 1892, 2085, 2209, 2437, 2495, 3820
ApaLI GTGCAC 1 cut(s) 3797
ApoI RAATTY 5 cut(s) 112, 870, 1288, 1710, 2478
AseI ATTAAT 1 cut(s) 2114
Asp700I GAANNNNTTC 2 cut(s) 1684, 3073
AspLEI GCGC 3 cut(s) 497, 2625, 3902
AspS9I GGNCC 8 cut(s) 158, 2016, 2051, 2085, 2495, 3164, 3821, 4024
AsuC2I CCSGG 1 cut(s) 3763
AsuHPI GGTGA 4 cut(s) 497, 992, 1922, 3230
AsuII TTCGAA 2 cut(s) 1705, 3869
AsuNHI GCTAGC 1 cut(s) 2645
AvaII GGWCC 4 cut(s) 2016, 2051, 3164, 4024
AxyI CCTNAGG 1 cut(s) 906
BaeGI GKGCMC 2 cut(s) 1348, 3801
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BalI TGGCCA 3 cut(s) 737, 1894, 2211
BamHI GGATCC 1 cut(s) 3438
BanI GGYRCC 2 cut(s) 575, 3419
BanII GRGCYC 2 cut(s) 1534, 3259
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 2 cut(s) 1534, 3801
BbvCI CCTCAGC 1 cut(s) 3741
BccI CCATC 9 cut(s) 97, 101, 304, 371, 1462, 2251, 2887, 3420, 3566
BceAI ACGGC 3 cut(s) 455, 2489, 3305
BcgI CGANNNNNNTGC 2 cut(s) 2243, 2277
BciVI GTATCC 2 cut(s) 256, 2353
BclI TGATCA 1 cut(s) 2598
BcnI CCSGG 1 cut(s) 3763
BcoDI GTCTC 8 cut(s) 804, 887, 1170, 1805, 1881, 1915, 2738, 3826
BfaI CTAG 6 cut(s) 1259, 2562, 2646, 2705, 3161, 3650
BfmI CTRYAG 7 cut(s) 1764, 2145, 2352, 2792, 2853, 3372, 3468
BfoI RGCGCY 3 cut(s) 498, 2626, 3903
BfrI CTTAAG 3 cut(s) 1382, 1853, 3845
BfuAI ACCTGC 1 cut(s) 1757
BfuI GTATCC 2 cut(s) 256, 2353
BglII AGATCT 1 cut(s) 3526
BlpI GCTNAGC 1 cut(s) 746
BmcAI AGTACT 2 cut(s) 2143, 2247
Bme18I GGWCC 4 cut(s) 2016, 2051, 3164, 4024
BmgT120I GGNCC 8 cut(s) 158, 2016, 2051, 2085, 2495, 3164, 3821, 4024
BmiI GGNNCC 4 cut(s) 145, 577, 3421, 3440
BmrI ACTGGG 3 cut(s) 1877, 2258, 3674
BmtI GCTAGC 1 cut(s) 2649
BmuI ACTGGG 3 cut(s) 1877, 2258, 3674
BpiI GAAGAC 1 cut(s) 399
BpmI CTGGAG 4 cut(s) 1080, 1227, 1509, 2065
Bpu10I CCTNAGC 2 cut(s) 3741, 4001
Bpu1102I GCTNAGC 1 cut(s) 746
Bpu14I TTCGAA 2 cut(s) 1705, 3869
BpuEI CTTGAG 7 cut(s) 64, 551, 736, 1137, 1674, 2110, 3563
BpuMI CCSGG 1 cut(s) 3763
BsaBI GATNNNNATC 2 cut(s) 1965, 2337
BsaHI GRCGYC 2 cut(s) 5, 4037
BsaI GGTCTC 2 cut(s) 804, 2738
BsaJI CCNNGG 9 cut(s) 9, 596, 1338, 2082, 2697, 3135, 3252, 3508, 3972
BsaWI WCCGGW 4 cut(s) 410, 1364, 1846, 2957
BsaXI ACNNNNNCTCC 2 cut(s) 1222, 1252
Bsc4I CCNNNNNNNGG 7 cut(s) 166, 1316, 1341, 2082, 2356, 3818, 4033
Bse21I CCTNAGG 1 cut(s) 906
Bse3DI GCAATG 3 cut(s) 462, 984, 1524
Bse8I GATNNNNATC 2 cut(s) 1965, 2337
BseAI TCCGGA 1 cut(s) 410
BseDI CCNNGG 9 cut(s) 9, 596, 1338, 2082, 2697, 3135, 3252, 3508, 3972
BseJI GATNNNNATC 2 cut(s) 1965, 2337
BseLI CCNNNNNNNGG 7 cut(s) 166, 1316, 1341, 2082, 2356, 3818, 4033
BseMI GCAATG 3 cut(s) 462, 984, 1524
BseRI GAGGAG 5 cut(s) 59, 179, 2438, 2882, 3758
BseSI GKGCMC 2 cut(s) 1348, 3801
BsgI GTGCAG 4 cut(s) 1416, 1635, 2113, 2818
Bsh1236I CGCG 2 cut(s) 322, 2412
BshFI GGCC 9 cut(s) 159, 442, 737, 1894, 2087, 2211, 2439, 2497, 3822
BshNI GGYRCC 2 cut(s) 575, 3419
BsiHKAI GWGCWC 2 cut(s) 1534, 3801
BsiSI CCGG 6 cut(s) 411, 1365, 1847, 2958, 3395, 3762
BslFI GGGAC 4 cut(s) 2585, 3141, 3694, 3933
BslI CCNNNNNNNGG 7 cut(s) 166, 1316, 1341, 2082, 2356, 3818, 4033
BsmAI GTCTC 8 cut(s) 804, 887, 1170, 1805, 1881, 1915, 2738, 3826
BsmFI GGGAC 4 cut(s) 2585, 3141, 3694, 3933
BsmI GAATGC 6 cut(s) 1645, 2170, 3304, 3329, 3623, 3938
BsnI GGCC 9 cut(s) 159, 442, 737, 1894, 2087, 2211, 2439, 2497, 3822
Bso31I GGTCTC 2 cut(s) 804, 2738
Bsp119I TTCGAA 2 cut(s) 1705, 3869
Bsp1286I GDGCHC 4 cut(s) 1348, 1534, 3259, 3801
Bsp13I TCCGGA 1 cut(s) 410
Bsp1407I TGTACA 1 cut(s) 3496
Bsp1720I GCTNAGC 1 cut(s) 746
Bsp19I CCATGG 1 cut(s) 1338
Bsp68I TCGCGA 1 cut(s) 2412
BspANI GGCC 9 cut(s) 159, 442, 737, 1894, 2087, 2211, 2439, 2497, 3822
BspEI TCCGGA 1 cut(s) 410
BspFNI CGCG 2 cut(s) 322, 2412
BspHI TCATGA 1 cut(s) 1990
BspLI GGNNCC 4 cut(s) 145, 577, 3421, 3440
BspMAI CTGCAG 5 cut(s) 1768, 2149, 2356, 3376, 3472
BspMI ACCTGC 1 cut(s) 1757
BspOI GCTAGC 1 cut(s) 2649
BspQI GCTCTTC 1 cut(s) 2775
BspT104I TTCGAA 2 cut(s) 1705, 3869
BspT107I GGYRCC 2 cut(s) 575, 3419
BspTI CTTAAG 3 cut(s) 1382, 1853, 3845
BspTNI GGTCTC 2 cut(s) 804, 2738
BsrDI GCAATG 3 cut(s) 462, 984, 1524
BsrGI TGTACA 1 cut(s) 3496
BssECI CCNNGG 9 cut(s) 9, 596, 1338, 2082, 2697, 3135, 3252, 3508, 3972
BssNI GRCGYC 2 cut(s) 5, 4037
BssT1I CCWWGG 3 cut(s) 9, 1338, 2697
Bst6I CTCTTC 3 cut(s) 1751, 2775, 2853
BstACI GRCGYC 2 cut(s) 5, 4037
BstAFI CTTAAG 3 cut(s) 1382, 1853, 3845
BstAPI GCANNNNNTGC 5 cut(s) 887, 3113, 3368, 3629, 4067
BstAUI TGTACA 1 cut(s) 3496
BstBI TTCGAA 2 cut(s) 1705, 3869
BstC8I GCNNGC 8 cut(s) 919, 1271, 1485, 2437, 2621, 2647, 3647, 3738
BstDSI CCRYGG 3 cut(s) 596, 1338, 3508
BstFNI CGCG 2 cut(s) 322, 2412
BstH2I RGCGCY 3 cut(s) 498, 2626, 3903
BstHHI GCGC 3 cut(s) 497, 2625, 3902
BstMAI GTCTC 8 cut(s) 804, 887, 1170, 1805, 1881, 1915, 2738, 3826
BstNSI RCATGY 3 cut(s) 1624, 2547, 3456
BstSFI CTRYAG 7 cut(s) 1764, 2145, 2352, 2792, 2853, 3372, 3468
BstSLI GKGCMC 2 cut(s) 1348, 3801
BstUI CGCG 2 cut(s) 322, 2412
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 8 cut(s) 730, 1672, 1900, 2701, 3408, 3438, 3526, 3850
BstXI CCANNNNNNTGG 1 cut(s) 1890
BstYI RGATCY 8 cut(s) 730, 1672, 1900, 2701, 3408, 3438, 3526, 3850
Bsu36I CCTNAGG 1 cut(s) 906
BsuI GTATCC 2 cut(s) 256, 2353
BsuRI GGCC 9 cut(s) 159, 442, 737, 1894, 2087, 2211, 2439, 2497, 3822
BtgI CCRYGG 3 cut(s) 596, 1338, 3508
BtsI GCAGTG 3 cut(s) 82, 2637, 4068
BtuMI TCGCGA 1 cut(s) 2412
BveI ACCTGC 1 cut(s) 1757
Cac8I GCNNGC 8 cut(s) 919, 1271, 1485, 2437, 2621, 2647, 3647, 3738
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 1 cut(s) 1990
CfoI GCGC 3 cut(s) 497, 2625, 3902
Cfr13I GGNCC 8 cut(s) 158, 2016, 2051, 2085, 2495, 3164, 3821, 4024
CseI GACGC 6 cut(s) 328, 393, 1491, 2504, 3036, 4026
CsiI ACCWGGT 1 cut(s) 4020
Csp6I GTAC 5 cut(s) 1368, 2142, 2246, 3497, 3808
CspCI CAANNNNNGTGG 6 cut(s) 1057, 1092, 2757, 2792, 3105, 3140
CviQI GTAC 5 cut(s) 1368, 2142, 2246, 3497, 3808
DraI TTTAAA 3 cut(s) 384, 432, 1008
EaeI YGGCCR 4 cut(s) 440, 735, 1892, 2209
Eam1104I CTCTTC 3 cut(s) 1751, 2775, 2853
EarI CTCTTC 3 cut(s) 1751, 2775, 2853
EciI GGCGGA 3 cut(s) 2621, 3251, 3584
Ecl136II GAGCTC 1 cut(s) 1532
Eco130I CCWWGG 3 cut(s) 9, 1338, 2697
Eco24I GRGCYC 2 cut(s) 1534, 3259
Eco31I GGTCTC 2 cut(s) 804, 2738
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 4 cut(s) 2016, 2051, 3164, 4024
Eco47III AGCGCT 1 cut(s) 2624
Eco53kI GAGCTC 1 cut(s) 1532
Eco57I CTGAAG 1 cut(s) 1533
Eco81I CCTNAGG 1 cut(s) 906
EcoICRI GAGCTC 1 cut(s) 1532
EcoO109I RGGNCCY 3 cut(s) 2051, 2085, 3164
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 3 cut(s) 9, 1338, 2697
EcoT22I ATGCAT 4 cut(s) 2266, 2549, 3938, 4083
EcoT38I GRGCYC 2 cut(s) 1534, 3259
ErhI CCWWGG 3 cut(s) 9, 1338, 2697
FalI AAGNNNNNCTT 6 cut(s) 1827, 1859, 2612, 2644, 3276, 3308
FaqI GGGAC 4 cut(s) 2585, 3141, 3694, 3933
FauI CCCGC 2 cut(s) 622, 768
FauNDI CATATG 1 cut(s) 2752
FbaI TGATCA 1 cut(s) 2598
FriOI GRGCYC 2 cut(s) 1534, 3259
FspBI CTAG 6 cut(s) 1259, 2562, 2646, 2705, 3161, 3650
GlaI GCGC 3 cut(s) 496, 2624, 3901
GsuI CTGGAG 4 cut(s) 1080, 1227, 1509, 2065
HaeII RGCGCY 3 cut(s) 498, 2626, 3903
HaeIII GGCC 9 cut(s) 159, 442, 737, 1894, 2087, 2211, 2439, 2497, 3822
HapII CCGG 6 cut(s) 411, 1365, 1847, 2958, 3395, 3762
HgaI GACGC 6 cut(s) 328, 393, 1491, 2504, 3036, 4026
HhaI GCGC 3 cut(s) 497, 2625, 3902
Hin1I GRCGYC 2 cut(s) 5, 4037
Hin6I GCGC 3 cut(s) 495, 2623, 3900
HinP1I GCGC 3 cut(s) 495, 2623, 3900
HincII GTYRAC 2 cut(s) 949, 2554
HindII GTYRAC 2 cut(s) 949, 2554
HindIII AAGCTT 5 cut(s) 93, 134, 467, 1421, 3984
HpaI GTTAAC 1 cut(s) 2554
HpaII CCGG 6 cut(s) 411, 1365, 1847, 2958, 3395, 3762
HphI GGTGA 4 cut(s) 497, 992, 1922, 3230
Hpy166II GTNNAC 7 cut(s) 949, 2554, 3499, 3578, 3799, 3967, 4027
Hpy8I GTNNAC 7 cut(s) 949, 2554, 3499, 3578, 3799, 3967, 4027
Hpy99I CGWCG 4 cut(s) 32, 1507, 3704, 4042
HpyCH4IV ACGT 3 cut(s) 1863, 3046, 4029
HpySE526I ACGT 3 cut(s) 1863, 3046, 4029
Hsp92I GRCGYC 2 cut(s) 5, 4037
HspAI GCGC 3 cut(s) 495, 2623, 3900
Kpn2I TCCGGA 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 2598
KspAI GTTAAC 1 cut(s) 2554
LguI GCTCTTC 1 cut(s) 2775
MabI ACCWGGT 1 cut(s) 4020
MaeI CTAG 6 cut(s) 1259, 2562, 2646, 2705, 3161, 3650
MaeII ACGT 3 cut(s) 1863, 3046, 4029
MaeIII GTNAC 5 cut(s) 1138, 2458, 2564, 3047, 3218
MfeI CAATTG 1 cut(s) 1478
MflI RGATCY 8 cut(s) 730, 1672, 1900, 2701, 3408, 3438, 3526, 3850
MhlI GDGCHC 4 cut(s) 1348, 1534, 3259, 3801
MlsI TGGCCA 3 cut(s) 737, 1894, 2211
MluI ACGCGT 1 cut(s) 320
MluNI TGGCCA 3 cut(s) 737, 1894, 2211
MlyI GAGTC 3 cut(s) 1051, 3828, 3888
MmeI TCCRAC 3 cut(s) 937, 1994, 2290
Mox20I TGGCCA 3 cut(s) 737, 1894, 2211
Mph1103I ATGCAT 4 cut(s) 2266, 2549, 3938, 4083
MroI TCCGGA 1 cut(s) 410
MroXI GAANNNNTTC 2 cut(s) 1684, 3073
MscI TGGCCA 3 cut(s) 737, 1894, 2211
MslI CAYNNNNRTG 2 cut(s) 3417, 3974
Msp20I TGGCCA 3 cut(s) 737, 1894, 2211
MspA1I CMGCKG 4 cut(s) 338, 2237, 2591, 2888
MspCI CTTAAG 3 cut(s) 1382, 1853, 3845
MspI CCGG 6 cut(s) 411, 1365, 1847, 2958, 3395, 3762
MunI CAATTG 1 cut(s) 1478
Mva1269I GAATGC 6 cut(s) 1645, 2170, 3304, 3329, 3623, 3938
MvnI CGCG 2 cut(s) 322, 2412
NciI CCSGG 1 cut(s) 3763
NcoI CCATGG 1 cut(s) 1338
NdeI CATATG 1 cut(s) 2752
NheI GCTAGC 1 cut(s) 2645
NlaIV GGNNCC 4 cut(s) 145, 577, 3421, 3440
NmeAIII GCCGAG 1 cut(s) 2984
NmuCI GTSAC 3 cut(s) 1138, 2564, 3218
NruI TCGCGA 1 cut(s) 2412
NsiI ATGCAT 4 cut(s) 2266, 2549, 3938, 4083
NspI RCATGY 3 cut(s) 1624, 2547, 3456
NspV TTCGAA 2 cut(s) 1705, 3869
OliI CACNNNNGTG 1 cut(s) 3974
PacI TTAATTAA 1 cut(s) 2118
PagI TCATGA 1 cut(s) 1990
PciSI GCTCTTC 1 cut(s) 2775
PctI GAATGC 6 cut(s) 1645, 2170, 3304, 3329, 3623, 3938
PdmI GAANNNNTTC 2 cut(s) 1684, 3073
PflFI GACNNNGTC 2 cut(s) 1865, 4022
PflMI CCANNNNNTGG 1 cut(s) 4033
PfoI TCCNGGA 2 cut(s) 1240, 1592
PleI GAGTC 3 cut(s) 1051, 3828, 3887
PpsI GAGTC 3 cut(s) 1051, 3828, 3887
PpuMI RGGWCCY 2 cut(s) 2051, 3164
PshBI ATTAAT 1 cut(s) 2114
PsiI TTATAA 1 cut(s) 3926
Psp124BI GAGCTC 1 cut(s) 1534
Psp5II RGGWCCY 2 cut(s) 2051, 3164
PspN4I GGNNCC 4 cut(s) 145, 577, 3421, 3440
PspPI GGNCC 8 cut(s) 158, 2016, 2051, 2085, 2495, 3164, 3821, 4024
PspPPI RGGWCCY 2 cut(s) 2051, 3164
PstI CTGCAG 5 cut(s) 1768, 2149, 2356, 3376, 3472
PstNI CAGNNNCTG 1 cut(s) 1760
PsuI RGATCY 8 cut(s) 730, 1672, 1900, 2701, 3408, 3438, 3526, 3850
PsyI GACNNNGTC 2 cut(s) 1865, 4022
PvuII CAGCTG 3 cut(s) 338, 2591, 2888
RruI TCGCGA 1 cut(s) 2412
RsaI GTAC 5 cut(s) 1369, 2143, 2247, 3498, 3809
RsaNI GTAC 5 cut(s) 1368, 2142, 2246, 3497, 3808
RseI CAYNNNNRTG 2 cut(s) 3417, 3974
SacI GAGCTC 1 cut(s) 1534
SapI GCTCTTC 1 cut(s) 2775
Sau96I GGNCC 8 cut(s) 158, 2016, 2051, 2085, 2495, 3164, 3821, 4024
SbfI CCTGCAGG 1 cut(s) 2356
ScaI AGTACT 2 cut(s) 2143, 2247
SchI GAGTC 3 cut(s) 1051, 3828, 3888
SdaI CCTGCAGG 1 cut(s) 2356
SduI GDGCHC 4 cut(s) 1348, 1534, 3259, 3801
SexAI ACCWGGT 1 cut(s) 4020
SfcI CTRYAG 7 cut(s) 1764, 2145, 2352, 2792, 2853, 3372, 3468
SfuI TTCGAA 2 cut(s) 1705, 3869
SinI GGWCC 4 cut(s) 2016, 2051, 3164, 4024
SmiMI CAYNNNNRTG 2 cut(s) 3417, 3974
Sse8387I CCTGCAGG 1 cut(s) 2356
SspI AATATT 1 cut(s) 291
SspMI CTAG 6 cut(s) 1259, 2562, 2646, 2705, 3161, 3650
SstI GAGCTC 1 cut(s) 1534
StyI CCWWGG 3 cut(s) 9, 1338, 2697
TaiI ACGT 3 cut(s) 1866, 3049, 4032
TatI WGTACW 3 cut(s) 2141, 2245, 3496
TauI GCSGC 3 cut(s) 159, 2237, 2442
TseFI GTSAC 3 cut(s) 1138, 2564, 3218
Tsp45I GTSAC 3 cut(s) 1138, 2564, 3218
TspGWI ACGGA 8 cut(s) 585, 1191, 2024, 2048, 2742, 3525, 3816, 4007
Tth111I GACNNNGTC 2 cut(s) 1865, 4022
Van91I CCANNNNNTGG 1 cut(s) 4033
Vha464I CTTAAG 3 cut(s) 1382, 1853, 3845
VneI GTGCAC 1 cut(s) 3797
VpaK11BI GGWCC 4 cut(s) 2016, 2051, 3164, 4024
VspI ATTAAT 1 cut(s) 2114
XapI RAATTY 5 cut(s) 112, 870, 1288, 1710, 2478
XceI RCATGY 3 cut(s) 1624, 2547, 3456
XcmI CCANNNNNNNNNTGG 1 cut(s) 4060
XmnI GAANNNNTTC 2 cut(s) 1684, 3073
XspI CTAG 6 cut(s) 1259, 2562, 2646, 2705, 3161, 3650
ZrmI AGTACT 2 cut(s) 2143, 2247
Zsp2I ATGCAT 4 cut(s) 2266, 2549, 3938, 4083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.