RLG00000005461

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006366
Physical Location & Seq
Reverse (-)
67830851 .. 67843777
12927 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005461

Sequence Viewer

Length: 4116 bp
ATGGCGTTTCCTGGTGTTGTTGGGTTTGTGGGATTGGATGACTTGAGCTTGGACTTGGCTTCTTCACTTGTCCGCAGCGGTTACAGAGTTCAAGCATTTGAGACATATGAACCTTTGATAAATGAGCTTTTAAAGCTAGGGGGAACAAGATGTAGCAGCCCAAAGGAGGCAGGAAAAGATGTTTCAGCTTTGGTTCTGTTAACTTCTCAGGCAGATCAAATTAATGATGCAACGTCAGGATTGCAGAACGATACTGTTCTCATTTTTAATTCAACAATGTTACCGTTGTACATTAAGAATTTGCAGACGTCTTTCACAGCTGATTGTAAGCCAGCTTATGTTGTAGATGTATATGCCATGAAGGCAGTATCTGATAGTTTAAATGGAAAAATCATGATTGCTTCTTCAGGAAGTTCAAATGCCATTTTAAAGGCACGACCGGTTCTCTCTGCAATGTGTGAAAAGCTTTATGTTTTTGAGGGTGAAGTTGGTGCTGGAAGCAAACTTAAGATGGTAAAGGAACTGCTTGAGGGTATTCATCTTGTTGCATCTTTGGAAGCTATTTCTCTTGGTACCAAAGCTGGTATTCATCCGTGGATAATCTATGATATCATATCTAATGCGGCCGGAAACTCATGGGTTTTTAAGAACCAGGTGCCCCAGTTGTTAAAGCACTCAAAGGGTGCTCCTAAAGATCATTTATGCGACACTCTGGTTCAAAAATTGGGGGTTATATTGAATCTTGCCAAATCGCTTACTTTCCCTCTGCCACTTTTAGCAGTAGCTCACCAACATCTTATTCTTGGGTCTTCAGATGGTAACACAGATGATCAAGATGCCACATTGATTAAGGTTTGGGAAAAGAAACTTGGAGTAAAAATTTCAGATGCAGCAAATGCTGAGACTTACATTCCTGAGCAATTGGCGAGCCATATTGTTGCTAAATCAGATATGGTGAAGCGGATTGGATTCATTGGTCTTGGAGCAATGGGATTCGGCATGGCAACTCAACTTCTAAAATCAAACTTTTGTGTTCTTGGTTATGATGTATATAAACCAACACTAACGCGATTTGCCAATGCTGGTGGCTTGATTGGAAGCTCTCCAGCAGAAGTATGTAAAGATGTCGATGTTCTGGTGATAATGGTCACAAATGAAGCTCAAGCTGAGAGCGCTTTATTTGGAGATTTTGGTGCTATTTCAGCACTTCCATCTGGAGCTTCTATTATCCTTTCATCCACTGTTTCTCCTGGATTTGTGAGCCAACTAGATCAACGCTTACAAAATGAAGGAAAGGATCTGAAGTTGGTGGATGCTCCTGTTTCTGGTGGTGTTGTGAGAGCCGGCAATGGGACACTTACGATCATGGCATCTGGCACAGATGAAGCTCTTAAGAGTACTGGTTCAGTCCTCTCAGCATTAAGCGAGAAGCTTTATATTATCAAAGGAGGCTGTGGAGCTGGAAGTGGTGTAAAGATGGTTAATCAATTGCTAGCTGGAGTTCATATAGCATCGGGAGCTGAGGCAATGGCATTTGGAGCTCGGTTGGGTCTTAATACAAGGATGTTATTTGATTTCATCACAAATAGTGAGGGATCATCCTGGATGTTTGAAAATCGTGTTCCACACATGCTGGACAATGATTATACACCACTTTCTGCGCTTGATATCTTTGTGAAAGATTTGGGAATTGTTACTCACGAATGTTCAATTCGAAAGGTTCCACTTCATGTATCAACTATTGCTCATCAACTATTCCTATCAGGTTCTGCTGCAGGCTGGGGTCGACAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTCACAGGCGTTAAGGTAGAAGGAAAACTTCCGGCTGTTAAGAAAGACTTTGTATTGCAATCTCTCCCAGCTGAGTGGCCATTAGATCCCATAGGTGAAATACAGAAACTAAATCAGGACACATCAAAGACTTTGGTTGTTCTTGATGATGATCCGACTGGAACTCAAACTGTGCATGATATTGAGGTCTTAACTGAATGGACTGTTGAATCACTAATTGAGCAGTTCAGAAAAAGCCCGAAATGCTTTTTCATTTTGACCAACTCCAGGGCAATGAGCTCTGACAAGGCCACCACATTAATCAAAGACATCTGCACAAACTTGCATACTGCAGCCAAATCAGTTCAGGACGCTGATTATACTGTAGTTTTGAGAGGTGATTCGACTTTGCGTGGTCATTTCCCAGAGGCAACTGATGCTGCTGTTTCAGTATTGGGTGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGCGGGCGTTATACCATCGGAGACATACATTATGTGGCTGACACTGATCAGCTTATCCCTGCAGCAGATACAGGATTTGCTAAAGATGCTGCTTTTGGCTACAAATCTTCGAATCTTCGTGAGTGGGTTGAGGAGAAAACAGCTGGTCGCATACCAGCCAGCAGTGTCGCATCCATTTCTATTCAACTGCTGAGAGAAGGTGGGCCAGATGCTGTTTGTGAGCATCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAATGCTGCTAGCGAAAGAGATATGGCTGTTTTTGCAGCTGGAATGATCAAGGCAGAACTAAAAGGCAAGAAATTCTTATGCCGCACTGCTGCTAGTTTTGTTTCTGCAAGGATTGGAATTATTCCCAAAGCTCCAATTTTGCCCAAGGATATAGGAATAAACAAGGAACAGAATGGTGGTCTCATAGTTGTTGGATCATATGTTGCAAAGACGACAAAACAGGTAGAAGAGCTTAGGTTACAATGTGGCCAGATTCTGAGAAGCATTGAGGTTTCGGTTGCTAAAGTTGCAATGCAGTCTGCAGAAGAGAGGGAGGAGGAAATCAGTACAGCAGCTGAGATGGCTGATATTTTTCTTGCAGCTCAGAATGACACACTAATAGTAACCAGTCGGGGGCTTATTACCGGCAAAACTCCCTCTGAGAGTTTGGAAATCAACTTCAAAGTTAGCTCTGCATTGGTGGAAATAGTTAGGCGAATAACTAAAAGACCTCGTTACATCCTTGCAAAGGGTGGAATCACATCTTCTGACCTTGCTACTAAAGCCCTTGAAGCAAAATGTGCTAAAATAGTGGGACAAGCCTTGCCTGGTGTTCCCTTATGGCAGTTGGGTCCTGAAAGTAGGCATCTGGGAGTTCCATACATTGTTTTTCCCGGTAATGTTGGTGACAGTGGAGCATTGGCCGAGCTAGTGAAATCCTGGGCTCGTCCAATCAGATTTTCAACAAAAGAGCTTCTTCTGAACGCAGAAAAGGGTGGATATGCTGTTGGAGCATTCAATGTTTACAATTTAGAAGGAGTAGAGGCAGTTGTTGCTGCAGCGGAGGAACAACAGAGTCCTGCCATATTACAGATCCATCCCAGTGCCTTGAAGCAAGGAGGCATGCCGTTGATTGCTTGTTGCATCTCTGCTGCTGAACAAGCCAGTGTACCCATAACTGTACACTTTGATCATGGAACTTCAAAGCAAGATCTGGTGACAGCTCTTGAGTTGGGATTTGATTCTGTCATGGTAGATGGTTCACATCTTTCCTTTACAGAAAATGTTTTGTACACTAGGTTCATAACATTCTTGGCTCACTCAAAAGGCTTGCTAGTAGAAGCTGAACTAGGGAGATTGTCAGGGACAGAAGATGATCTGACTGTTGAAGATTATGAAGCAAGGCTGACCGATGTTAAGCAGGCTCAAGAGTTCATCGATGAAACTGGTATAGATGCTTTGGCAGTGTGTATCGGAAATGTCCATGGTAAATACCCTGCAAGTGGCCCAAATCTGAGACTTGACTTGCTTAAGGAATTGCACGCGTTGAGTTCAAGGAAAGGAGTCTTTCTGGTGCTTCATGGAGCTTCTGGTGTGCCCAAGGAACTTGTAAAGGGATGCATAGAGCTAGGTGTGAGAAAGTTCAATGTAAATACCGAGGTACGGAAAGCTTATATGGATTCCCTTAGCAACTCAAAGAAAGATCTGGTCCATGTTATGGAGTCAGCAAAACTAGCCATGAAAGCAGTGATTGCAGAGAAGATGATTCTATTTGGCTCAGCAGGAAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1372

Amino Acids

146.52

Weight (kDa)

5.83

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 7 - 157 2.4e-07 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 164 - 285 4.2e-18 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 321 - 479 8.4e-36 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 486 - 606 8.8e-29 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 653 - 890 9.8e-58 Sugar-binding N-terminal domain
NBD_C PF17042 915 - 1081 7.3e-34 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1095 - 1370 5.7e-91 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 311
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 2 cut(s) 572, 655
AccB7I CCANNNNNTGG 1 cut(s) 4039
AccI GTMKAC 1 cut(s) 1786
AccII CGCG 2 cut(s) 1069, 3866
AciI CCGC 7 cut(s) 73, 78, 623, 961, 2304, 2644, 3383
AclWI GGATC 8 cut(s) 1305, 1603, 1904, 1970, 2285, 2554, 2764, 3409
AcoI YGGCCR 4 cut(s) 624, 1901, 2809, 3243
AcsI RAATTY 3 cut(s) 298, 879, 2633
AcuI CTGAAG 3 cut(s) 390, 795, 1322
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 8 cut(s) 290, 574, 1399, 2890, 3492, 3504, 3614, 3984
AfeI AGCGCT 1 cut(s) 1174
AfiI CCNNNNNNNGG 9 cut(s) 166, 1325, 1350, 2091, 2955, 3070, 3824, 3984, 4039
AflII CTTAAG 3 cut(s) 506, 1391, 3851
AflIII ACRYGT 1 cut(s) 3864
AgeI ACCGGT 1 cut(s) 439
AjnI CCWGG 7 cut(s) 10, 651, 1249, 1601, 2090, 3148, 3260
AjuI GAANNNNNNNTTGG 6 cut(s) 783, 815, 852, 884, 3265, 3297
Alw21I GWGCWC 3 cut(s) 688, 1543, 2105
Alw26I GTCTC 6 cut(s) 95, 896, 1814, 2316, 2747, 3832
AlwI GGATC 8 cut(s) 1305, 1603, 1904, 1970, 2285, 2554, 2764, 3409
AlwNI CAGNNNCTG 5 cut(s) 371, 1769, 2513, 2818, 2897
Aor51HI AGCGCT 1 cut(s) 1174
AoxI GGCC 7 cut(s) 624, 1901, 2112, 2504, 2809, 3243, 3826
ApoI RAATTY 3 cut(s) 298, 879, 2633
AseI ATTAAT 2 cut(s) 222, 2123
AsiGI ACCGGT 1 cut(s) 439
Asp718I GGTACC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 1175, 1663
AspS9I GGNCC 4 cut(s) 2504, 3173, 3827, 4030
AsuC2I CCSGG 1 cut(s) 3216
AsuHPI GGTGA 9 cut(s) 494, 779, 967, 1150, 1931, 2213, 2273, 3239, 3550
AsuII TTCGAA 2 cut(s) 1714, 2411
AsuNHI GCTAGC 2 cut(s) 1492, 2570
AvaII GGWCC 2 cut(s) 3173, 4030
BaeGI GKGCMC 2 cut(s) 660, 3921
BalI TGGCCA 2 cut(s) 1903, 2811
BanI GGYRCC 2 cut(s) 572, 655
BanII GRGCYC 3 cut(s) 1543, 2105, 3268
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 3 cut(s) 688, 1543, 2105
BbvCI CCTCAGC 1 cut(s) 1521
BccI CCATC 9 cut(s) 505, 809, 1219, 1471, 2260, 2324, 2896, 3426, 3572
BceAI ACGGC 1 cut(s) 3433
BciT130I CCWGG 7 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3262
BclI TGATCA 4 cut(s) 829, 2347, 2607, 3511
BcnI CCSGG 1 cut(s) 3216
BcoDI GTCTC 6 cut(s) 95, 896, 1814, 2316, 2747, 3832
BfmI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
BfoI RGCGCY 1 cut(s) 1176
BfrI CTTAAG 3 cut(s) 506, 1391, 3851
BglI GCCNNNNNGGC 1 cut(s) 362
BglII AGATCT 2 cut(s) 3532, 4024
BlpI GCTNAGC 1 cut(s) 4099
BmcAI AGTACT 1 cut(s) 1399
Bme1390I CCNGG 8 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3216, 3262
Bme18I GGWCC 2 cut(s) 3173, 4030
BmgT120I GGNCC 4 cut(s) 2504, 3173, 3827, 4030
BmiI GGNNCC 4 cut(s) 574, 657, 1722, 3174
BmrFI CCNGG 8 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3216, 3262
BmrI ACTGGG 2 cut(s) 655, 3417
BmtI GCTAGC 2 cut(s) 1496, 2574
BmuI ACTGGG 2 cut(s) 655, 3417
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 4 cut(s) 1089, 1236, 1518, 2074
Bpu10I CCTNAGC 4 cut(s) 915, 1521, 2795, 4007
Bpu1102I GCTNAGC 1 cut(s) 4099
Bpu14I TTCGAA 2 cut(s) 1714, 2411
BpuEI CTTGAG 5 cut(s) 64, 548, 1146, 3569, 3732
BpuMI CCSGG 1 cut(s) 3216
Bsa29I ATCGAT 1 cut(s) 3759
BsaBI GATNNNNATC 1 cut(s) 1974
BsaHI GRCGYC 1 cut(s) 308
BsaI GGTCTC 1 cut(s) 2747
BsaJI CCNNGG 7 cut(s) 593, 2091, 2706, 3261, 3805, 3921, 3978
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 9 cut(s) 166, 1325, 1350, 2091, 2955, 3070, 3824, 3984, 4039
Bse118I RCCGGY 3 cut(s) 439, 1343, 2966
Bse1I ACTGG 7 cut(s) 661, 1405, 1987, 2949, 3423, 3486, 3772
Bse3DI GCAATG 6 cut(s) 459, 993, 1354, 1533, 2103, 2859
Bse8I GATNNNNATC 1 cut(s) 1974
BseBI CCWGG 7 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3262
BseCI ATCGAT 1 cut(s) 3759
BseDI CCNNGG 7 cut(s) 593, 2091, 2706, 3261, 3805, 3921, 3978
BseJI GATNNNNATC 1 cut(s) 1974
BseLI CCNNNNNNNGG 9 cut(s) 166, 1325, 1350, 2091, 2955, 3070, 3824, 3984, 4039
BseMI GCAATG 6 cut(s) 459, 993, 1354, 1533, 2103, 2859
BseNI ACTGG 7 cut(s) 661, 1405, 1987, 2949, 3423, 3486, 3772
BseRI GAGGAG 2 cut(s) 2447, 2891
BseSI GKGCMC 2 cut(s) 660, 3921
BseX3I CGGCCG 1 cut(s) 624
BseYI CCCAGC 2 cut(s) 1779, 1891
BsgI GTGCAG 1 cut(s) 2122
Bsh1236I CGCG 2 cut(s) 1069, 3866
Bsh1285I CGRYCG 2 cut(s) 440, 627
BshFI GGCC 7 cut(s) 626, 1903, 2114, 2506, 2811, 3245, 3828
BshNI GGYRCC 2 cut(s) 572, 655
BshTI ACCGGT 1 cut(s) 439
BshVI ATCGAT 1 cut(s) 3759
BsiEI CGRYCG 2 cut(s) 440, 627
BsiHKAI GWGCWC 3 cut(s) 688, 1543, 2105
BsiSI CCGG 6 cut(s) 440, 627, 1344, 1856, 2967, 3216
BslFI GGGAC 3 cut(s) 1366, 3150, 3700
BslI CCNNNNNNNGG 9 cut(s) 166, 1325, 1350, 2091, 2955, 3070, 3824, 3984, 4039
BsmAI GTCTC 6 cut(s) 95, 896, 1814, 2316, 2747, 3832
BsmFI GGGAC 3 cut(s) 1366, 3150, 3700
BsmI GAATGC 1 cut(s) 3335
BsnI GGCC 7 cut(s) 626, 1903, 2114, 2506, 2811, 3245, 3828
Bso31I GGTCTC 1 cut(s) 2747
Bsp119I TTCGAA 2 cut(s) 1714, 2411
Bsp1286I GDGCHC 6 cut(s) 660, 688, 1543, 2105, 3268, 3921
Bsp1407I TGTACA 3 cut(s) 288, 3502, 3612
Bsp1720I GCTNAGC 1 cut(s) 4099
Bsp19I CCATGG 1 cut(s) 3805
BspACI CCGC 7 cut(s) 73, 78, 623, 961, 2304, 2644, 3383
BspANI GGCC 7 cut(s) 626, 1903, 2114, 2506, 2811, 3245, 3828
BspDI ATCGAT 1 cut(s) 3759
BspFNI CGCG 2 cut(s) 1069, 3866
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 4 cut(s) 574, 657, 1722, 3174
BspMAI CTGCAG 5 cut(s) 1777, 2158, 2365, 2866, 3382
BspOI GCTAGC 2 cut(s) 1496, 2574
BspPI GGATC 8 cut(s) 1305, 1603, 1904, 1970, 2285, 2554, 2764, 3409
BspQI GCTCTTC 1 cut(s) 2784
BspT104I TTCGAA 2 cut(s) 1714, 2411
BspT107I GGYRCC 2 cut(s) 572, 655
BspTI CTTAAG 3 cut(s) 506, 1391, 3851
BspTNI GGTCTC 1 cut(s) 2747
BsrDI GCAATG 6 cut(s) 459, 993, 1354, 1533, 2103, 2859
BsrFI RCCGGY 3 cut(s) 439, 1343, 2966
BsrGI TGTACA 3 cut(s) 288, 3502, 3612
BsrI ACTGG 7 cut(s) 661, 1405, 1987, 2949, 3423, 3486, 3772
BssAI RCCGGY 3 cut(s) 439, 1343, 2966
BssECI CCNNGG 7 cut(s) 593, 2091, 2706, 3261, 3805, 3921, 3978
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 3 cut(s) 2706, 3805, 3921
Bst2UI CCWGG 7 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3262
Bst4CI ACNGT 9 cut(s) 256, 285, 1243, 1996, 2029, 2188, 3233, 3502, 3706
Bst6I CTCTTC 2 cut(s) 2784, 2862
BstACI GRCGYC 1 cut(s) 308
BstAFI CTTAAG 3 cut(s) 506, 1391, 3851
BstAPI GCANNNNNTGC 5 cut(s) 896, 2240, 3122, 3374, 4073
BstAUI TGTACA 3 cut(s) 288, 3502, 3612
BstBI TTCGAA 2 cut(s) 1714, 2411
BstDSI CCRYGG 2 cut(s) 593, 3805
BstENI CCTNNNNNAGG 1 cut(s) 3068
BstFNI CGCG 2 cut(s) 1069, 3866
BstH2I RGCGCY 1 cut(s) 1176
BstHHI GCGC 2 cut(s) 1175, 1663
BstMAI GTCTC 6 cut(s) 95, 896, 1814, 2316, 2747, 3832
BstMCI CGRYCG 2 cut(s) 440, 627
BstNI CCWGG 7 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3262
BstNSI RCATGY 3 cut(s) 1633, 2556, 3448
BstSCI CCNGG 8 cut(s) 10, 651, 1249, 1601, 2090, 3148, 3214, 3260
BstSFI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
BstSLI GKGCMC 2 cut(s) 660, 3921
BstUI CGCG 2 cut(s) 1069, 3866
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 5 cut(s) 1297, 1909, 3414, 3532, 4024
BstXI CCANNNNNNTGG 1 cut(s) 1899
BstYI RGATCY 5 cut(s) 1297, 1909, 3414, 3532, 4024
BstZI CGGCCG 1 cut(s) 624
Bsu15I ATCGAT 1 cut(s) 3759
BsuRI GGCC 7 cut(s) 626, 1903, 2114, 2506, 2811, 3245, 3828
BsuTUI ATCGAT 1 cut(s) 3759
BtgI CCRYGG 2 cut(s) 593, 3805
BtsI GCAGTG 4 cut(s) 2470, 2646, 3792, 4074
BtsIMutI CAGTG 9 cut(s) 1239, 2343, 2470, 2646, 3238, 3430, 3493, 3792, 4074
CaiI CAGNNNCTG 5 cut(s) 371, 1769, 2513, 2818, 2897
CciI TCATGA 1 cut(s) 393
CfoI GCGC 2 cut(s) 1175, 1663
Cfr10I RCCGGY 3 cut(s) 439, 1343, 2966
Cfr13I GGNCC 4 cut(s) 2504, 3173, 3827, 4030
ClaI ATCGAT 1 cut(s) 3759
CseI GACGC 1 cut(s) 2183
CsiI ACCWGGT 1 cut(s) 651
Csp6I GTAC 8 cut(s) 289, 573, 1398, 2889, 3491, 3503, 3613, 3983
CspAI ACCGGT 1 cut(s) 439
CspCI CAANNNNNGTGG 2 cut(s) 1066, 1101
CviQI GTAC 8 cut(s) 289, 573, 1398, 2889, 3491, 3503, 3613, 3983
DraI TTTAAA 3 cut(s) 132, 381, 429
EaeI YGGCCR 4 cut(s) 624, 1901, 2809, 3243
EagI CGGCCG 1 cut(s) 624
Eam1104I CTCTTC 2 cut(s) 2784, 2862
EarI CTCTTC 2 cut(s) 2784, 2862
Ecl136II GAGCTC 2 cut(s) 1541, 2103
EclXI CGGCCG 1 cut(s) 624
Eco130I CCWWGG 3 cut(s) 2706, 3805, 3921
Eco24I GRGCYC 3 cut(s) 1543, 2105, 3268
Eco31I GGTCTC 1 cut(s) 2747
Eco32I GATATC 2 cut(s) 610, 1669
Eco47I GGWCC 2 cut(s) 3173, 4030
Eco47III AGCGCT 1 cut(s) 1174
Eco52I CGGCCG 1 cut(s) 624
Eco53kI GAGCTC 2 cut(s) 1541, 2103
Eco57I CTGAAG 3 cut(s) 390, 795, 1322
EcoICRI GAGCTC 2 cut(s) 1541, 2103
EcoNI CCTNNNNNAGG 1 cut(s) 3068
EcoO109I RGGNCCY 1 cut(s) 3173
EcoRII CCWGG 7 cut(s) 10, 651, 1249, 1601, 2090, 3148, 3260
EcoRV GATATC 2 cut(s) 610, 1669
EcoT14I CCWWGG 3 cut(s) 2706, 3805, 3921
EcoT22I ATGCAT 3 cut(s) 2275, 2558, 3944
EcoT38I GRGCYC 3 cut(s) 1543, 2105, 3268
ErhI CCWWGG 3 cut(s) 2706, 3805, 3921
FalI AAGNNNNNCTT 8 cut(s) 1836, 1868, 1856, 1888, 2621, 2653, 3282, 3314
FaqI GGGAC 3 cut(s) 1366, 3150, 3700
FauI CCCGC 1 cut(s) 2297
FauNDI CATATG 2 cut(s) 106, 2761
FbaI TGATCA 4 cut(s) 829, 2347, 2607, 3511
FblI GTMKAC 1 cut(s) 1786
FriOI GRGCYC 3 cut(s) 1543, 2105, 3268
GlaI GCGC 2 cut(s) 1174, 1662
GsaI CCCAGC 2 cut(s) 1783, 1895
GsuI CTGGAG 4 cut(s) 1089, 1236, 1518, 2074
HaeII RGCGCY 1 cut(s) 1176
HaeIII GGCC 7 cut(s) 626, 1903, 2114, 2506, 2811, 3245, 3828
HapII CCGG 6 cut(s) 440, 627, 1344, 1856, 2967, 3216
HgaI GACGC 1 cut(s) 2183
HhaI GCGC 2 cut(s) 1175, 1663
Hin1I GRCGYC 1 cut(s) 308
Hin6I GCGC 2 cut(s) 1173, 1661
HinP1I GCGC 2 cut(s) 1173, 1661
HincII GTYRAC 2 cut(s) 201, 1787
HindII GTYRAC 2 cut(s) 201, 1787
HindIII AAGCTT 3 cut(s) 464, 1430, 3990
HpaI GTTAAC 1 cut(s) 201
HpaII CCGG 6 cut(s) 440, 627, 1344, 1856, 2967, 3216
HphI GGTGA 9 cut(s) 494, 779, 967, 1150, 1931, 2213, 2273, 3239, 3550
Hpy166II GTNNAC 7 cut(s) 201, 1787, 3346, 3491, 3505, 3584, 3615
Hpy8I GTNNAC 7 cut(s) 201, 1787, 3346, 3491, 3505, 3584, 3615
HpyAV CCTTC 6 cut(s) 355, 1283, 1838, 2492, 2536, 3350
HpyCH4III ACNGT 9 cut(s) 256, 285, 1243, 1996, 2029, 2188, 3233, 3502, 3706
HpyCH4IV ACGT 2 cut(s) 233, 308
HpySE526I ACGT 2 cut(s) 233, 308
Hsp92I GRCGYC 1 cut(s) 308
HspAI GCGC 2 cut(s) 1173, 1661
KpnI GGTACC 1 cut(s) 576
KroI GCCGGC 1 cut(s) 1343
KroNI GCCGGC 1 cut(s) 1345
Ksp22I TGATCA 4 cut(s) 829, 2347, 2607, 3511
KspAI GTTAAC 1 cut(s) 201
LguI GCTCTTC 1 cut(s) 2784
MabI ACCWGGT 1 cut(s) 651
MaeII ACGT 2 cut(s) 233, 308
MfeI CAATTG 2 cut(s) 920, 1487
MflI RGATCY 5 cut(s) 1297, 1909, 3414, 3532, 4024
MhlI GDGCHC 6 cut(s) 660, 688, 1543, 2105, 3268, 3921
MlsI TGGCCA 2 cut(s) 1903, 2811
MluI ACGCGT 1 cut(s) 3864
MluNI TGGCCA 2 cut(s) 1903, 2811
MlyI GAGTC 3 cut(s) 3406, 3894, 4052
MmeI TCCRAC 3 cut(s) 2003, 2734, 3310
Mox20I TGGCCA 2 cut(s) 1903, 2811
Mph1103I ATGCAT 3 cut(s) 2275, 2558, 3944
MroNI GCCGGC 1 cut(s) 1343
MscI TGGCCA 2 cut(s) 1903, 2811
MslI CAYNNNNRTG 1 cut(s) 3423
Msp20I TGGCCA 2 cut(s) 1903, 2811
MspA1I CMGCKG 7 cut(s) 78, 320, 1895, 2444, 2600, 2897, 3383
MspCI CTTAAG 3 cut(s) 506, 1391, 3851
MspI CCGG 6 cut(s) 440, 627, 1344, 1856, 2967, 3216
MspR9I CCNGG 8 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3216, 3262
MunI CAATTG 2 cut(s) 920, 1487
Mva1269I GAATGC 1 cut(s) 3335
MvaI CCWGG 7 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3262
MvnI CGCG 2 cut(s) 1069, 3866
NaeI GCCGGC 1 cut(s) 1345
NciI CCSGG 1 cut(s) 3216
NcoI CCATGG 1 cut(s) 3805
NdeI CATATG 2 cut(s) 106, 2761
NgoMIV GCCGGC 1 cut(s) 1343
NheI GCTAGC 2 cut(s) 1492, 2570
NlaIV GGNNCC 4 cut(s) 574, 657, 1722, 3174
NmeAIII GCCGAG 1 cut(s) 3271
NmuCI GTSAC 3 cut(s) 1147, 3227, 3538
NsiI ATGCAT 3 cut(s) 2275, 2558, 3944
NspI RCATGY 3 cut(s) 1633, 2556, 3448
NspV TTCGAA 2 cut(s) 1714, 2411
PaeI GCATGC 1 cut(s) 3448
PagI TCATGA 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 2784
PctI GAATGC 1 cut(s) 3335
PdiI GCCGGC 1 cut(s) 1345
PflMI CCANNNNNTGG 1 cut(s) 4039
PfoI TCCNGGA 2 cut(s) 1249, 1601
PinAI ACCGGT 1 cut(s) 439
PleI GAGTC 3 cut(s) 3405, 3893, 4051
PpsI GAGTC 3 cut(s) 3405, 3893, 4051
PpuMI RGGWCCY 1 cut(s) 3173
PshBI ATTAAT 2 cut(s) 222, 2123
Psp124BI GAGCTC 2 cut(s) 1543, 2105
Psp5II RGGWCCY 1 cut(s) 3173
Psp6I CCWGG 7 cut(s) 10, 651, 1249, 1601, 2090, 3148, 3260
PspFI CCCAGC 2 cut(s) 1779, 1891
PspGI CCWGG 7 cut(s) 10, 651, 1249, 1601, 2090, 3148, 3260
PspN4I GGNNCC 4 cut(s) 574, 657, 1722, 3174
PspPI GGNCC 4 cut(s) 2504, 3173, 3827, 4030
PspPPI RGGWCCY 1 cut(s) 3173
PsrI GAACNNNNNNTAC 2 cut(s) 3605, 3637
PstI CTGCAG 5 cut(s) 1777, 2158, 2365, 2866, 3382
PstNI CAGNNNCTG 5 cut(s) 371, 1769, 2513, 2818, 2897
PsuI RGATCY 5 cut(s) 1297, 1909, 3414, 3532, 4024
PvuII CAGCTG 5 cut(s) 320, 1895, 2444, 2600, 2897
RsaI GTAC 8 cut(s) 290, 574, 1399, 2890, 3492, 3504, 3614, 3984
RsaNI GTAC 8 cut(s) 289, 573, 1398, 2889, 3491, 3503, 3613, 3983
RseI CAYNNNNRTG 1 cut(s) 3423
SacI GAGCTC 2 cut(s) 1543, 2105
SalI GTCGAC 1 cut(s) 1785
SapI GCTCTTC 1 cut(s) 2784
Sau96I GGNCC 4 cut(s) 2504, 3173, 3827, 4030
ScaI AGTACT 1 cut(s) 1399
SchI GAGTC 3 cut(s) 3406, 3894, 4052
ScrFI CCNGG 8 cut(s) 12, 653, 1251, 1603, 2092, 3150, 3216, 3262
SduI GDGCHC 6 cut(s) 660, 688, 1543, 2105, 3268, 3921
SexAI ACCWGGT 1 cut(s) 651
SfcI CTRYAG 6 cut(s) 1773, 2154, 2187, 2361, 2862, 3378
SfuI TTCGAA 2 cut(s) 1714, 2411
SinI GGWCC 2 cut(s) 3173, 4030
SmiMI CAYNNNNRTG 1 cut(s) 3423
SmlI CTYRAG 8 cut(s) 43, 506, 527, 1161, 1391, 3548, 3747, 3851
SmoI CTYRAG 8 cut(s) 43, 506, 527, 1161, 1391, 3548, 3747, 3851
SphI GCATGC 1 cut(s) 3448
SsiI CCGC 7 cut(s) 73, 78, 623, 961, 2304, 2644, 3383
SstI GAGCTC 2 cut(s) 1543, 2105
StyD4I CCNGG 8 cut(s) 10, 651, 1249, 1601, 2090, 3148, 3214, 3260
StyI CCWWGG 3 cut(s) 2706, 3805, 3921
TaaI ACNGT 9 cut(s) 256, 285, 1243, 1996, 2029, 2188, 3233, 3502, 3706
TaiI ACGT 2 cut(s) 236, 311
TaqI TCGA 6 cut(s) 1128, 1714, 1786, 2207, 2411, 3759
TaqII GACCGA 1 cut(s) 3746
TatI WGTACW 5 cut(s) 288, 1397, 2888, 3502, 3612
TauI GCSGC 2 cut(s) 626, 2646
TscAI CASTG 9 cut(s) 1246, 2350, 2470, 2653, 3238, 3430, 3493, 3792, 4074
TseFI GTSAC 3 cut(s) 1147, 3227, 3538
Tsp45I GTSAC 3 cut(s) 1147, 3227, 3538
TspGWI ACGGA 2 cut(s) 582, 4000
TspRI CASTG 9 cut(s) 1246, 2350, 2470, 2653, 3238, 3430, 3493, 3792, 4074
Van91I CCANNNNNTGG 1 cut(s) 4039
Vha464I CTTAAG 3 cut(s) 506, 1391, 3851
VpaK11BI GGWCC 2 cut(s) 3173, 4030
VspI ATTAAT 2 cut(s) 222, 2123
XagI CCTNNNNNAGG 1 cut(s) 3068
XapI RAATTY 3 cut(s) 298, 879, 2633
XceI RCATGY 3 cut(s) 1633, 2556, 3448
XmiI GTMKAC 1 cut(s) 1786
ZraI GACGTC 1 cut(s) 309
ZrmI AGTACT 1 cut(s) 1399
Zsp2I ATGCAT 3 cut(s) 2275, 2558, 3944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.