Rw7G001130

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006366
Physical Location & Seq
Forward (+)
934968 .. 947873
12906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001130.1

Sequence Viewer

Length: 4179 bp
ATGGCGTTTCATGGTGTTGTTGGGTTTGTGGGATTGGATGACTTGAGCTTGGACTTGGCTTCTTCACTTCTCCGCAGCGGTTACAGAGTTCAAGCATTTGAGACATATGAACCTTTGATAAATGAGTTTTTAAAGCTAGGGGGAACAAGATGTAGCAGCCCAAAGGAGGGGGAAAGGCTTGGGACACTGATATTCCTATTTCTCTGTCTGTCTCTTCTATGGCTTGCAGATGTTTCAGCTTTGGTTCTGTTAACTTCTCAGGCAGATCAAATTAATGATGCAACGTCAGGTAATGATGCTGCTTTTAAAAACGATACTGTTCTCATTTTTAATTCAACAATGTTACCATTGTACATTAAGAATTTGCAGACGTGTTTCACAGCTGATTATAAGCCAGCTTATGTTGTAGATGTATATGCCATGAAGGCAGTATCTGATAGTTTAAATGGAAAAATCATGATTGCTTCTTCAGGAAGTTCAAATGCCATTTTAAAGGCACGACCAGTTCTCTCTGCAATGTGTGAAAAGCTTTATGTTTTTGAGGGTGAAGTTGGTGCTGGAAGCAAAATTAAGATGGTTAAGGAACTGCTTGAGGGTATTCATCTTGTTGCATCTTTGGAAGCTATTTCTCTTGGTACCAAAGCTGGTATTCATCCGTGGATAATCTATGATATCATATCTAATGCGGCCGGAAACTCATGGGTTTTTAAGAACCAGGTGCCCCAGCTGTTAGAGCACTCAAAGGGTGCTCCTAAAGATCATTTACGCGACACTCTGGTTCAAAAATTGGGGGTTATATTGAATCTTGCCAAATCGCTTACTTTCCCTCTGCCACTTTTAGCAGTAGCTCACCAACATCTTATTCTTGGGTCTTCAGATGGTAACACAGATGATCAAGATGCCACATTGATTAAGGTTTGGGAAAAGAAACTTGGAGTAAAAATTTCAGATGCAGCAAATGCTGAGACTTACATCCCTGAGCAATTGGCGAGCCATATTGTTGCTAAATCAGATATGGTGAAGCGGATTGGATTCATTGGTCTTGGAGCAATGGGATTCGGCATGGCAACTCAACTTCTAAAATCAAACTTTTGTGTTCTTGGTTATGATGTATATAAACCGACACTAACGCGATTTGCCAATGCTGGTGGCTTGATTGGAAGCTCTCCAGCAGAAGTATGTAAAGATGTTGATGTTCTGGTAATAATGGTCACAAATGAAGCTCAAGCTGAGAGCGCTTTATTTGGAGATTTTGGTGCTATTTCAGCACTTCCATCTGGAGCTTCTATTATCCTTTCATCCACTGTTTCTCCTGGATTTGTGAGCCGACTAGATCAACGCTTACAAAATGAAGGAAAGGATCTGAAGTTGGTGGATGCTCCTGTTTCTGGTGGTGTTGTGAGAGCCGGCAATGGGACACTTACGATCATGGCATCTGGCACAGATGAAGCTCTTAAGAGTACTGGTTCAGTCCTCTCAGCATTAAGCGAGAGGCTTTATATTATCAAAGGAGGCTGTGGAGCTGGAAGTGGTGTAAAGATGGTTAATCAATTGCTAGCTGGAGTTCATATAGCATCGGGAGCTGAGGCAATGGCGTTTGGAGCTCGGTTGGGTCTTAATACGAGGATGTTATTTGATTTCATCACAAATAGTGAGGGATCATCCTGGATGTTTGAAAATCGTGTTCCACACATGCTGGACAATGATTATACACCACTTTCTGCGCTTGATATCTTTGTGAAAGATTTGGGAATTGTTACTCACGAATGTTCAATTCGAAAGGTTCCACTTCATGTATCGACTATTGCTCATCAACTATTCCTATCAGGTTCTGCTGCAGGCTGGGGTCGACAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTCACAGGCGTTAAGGTAGAAGGAAAACTTCCGGCTGTTAAGAAAGACTTTGTATTGCAATCTCTCCCAGCTGAATGGCCATTAGATCCCATAGGTGAAATACAGAAACTAAATCAGGACACATCAAAGACTTTGGTTGTTCTTGATGATGATCCGACTGGAACTCAAACTGTGCATGATATTGAGGTCTTAACTGAATGGACTGTTGAATCACTAATTGAGCAGTTCAGAAAAAGCCCGAAATGCTTTTTCATTTTGACCAACTCCAGGGCAATGAGCTCTGACAAGGCCACCACATTAATCAAAGACATCTGCACAAACTTGCATACTGCAGCCAAATCAGTTCAGGACGCTGATTATACTGTAGTTTTGAGAGGTGATTCGACTTTGCGTGGTCATTTCCCAGAGGAAGCTGATGCTGCTGTTTCAGTATTGGGTGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGCGGGCGTTATACCATCGGAGATATCCATTATGTGGCTGATACTGATCAGCTTATCCCTGCAGCAGATACAGGATTTGCTAAAGATGCTGCTTTTGGCTACAAATCTTCGAATCTTCGCGAGTGGGTTGAGGAGAAAACAGCTGGTCGCATACCAGCCAGCAGTGTCGCATCCATTTCTATTCAACTGCTGAGAGAAGGTGGGCCAGATGTTGTTTGTGAGCATCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAATGCTGCTAGCGAAAGAGATATGGCTGTTTTTGCAGCTGGAATGATCAAGGCAGAACTGAAAGGCAAGCAATTCTTATGCCGCACTGCTGCTAGTTTTGTTTCTGCAAGGATTGGAATTATTCCCAAAGCTCCAATTTTGCCCAAGGATCTAGGAATAAACAAGGAACGGAATGGTGGTCTCATAGTTGTTGGATCATATGTTTCAAAGACGACAAAACAGGTAGAAGAGCTTAAGTTACAATGTGGCCAGATTCTGAGAAGCATTGAGGTTTCGGTTGCTAAAGTTGCAATGAGGTCTGCAGAAGAGAGGGAGCAGGAAATCAGTACAGCAGCTGAGATGGCTGATATTTTTCTTGCAGCTCAGAATGACACACTAATAGTAACCAGTCGGGAGCTTATTACCGGCAAAAGTCCCTCTGAGAGTTTGGAAATCAACTTCAAAGTTAGCTCTGCACTGGTGGAAATAGTTAGGCGAATAACTAAAAGACCTCGTTACATCCTTGCAAAGGGTGGAATCACATCTTCTGACCTTGCTACTAAAGCCCTTGAAGCAAAATGTGCTAAAATAGTGGGACAAGCCTTGCCTGGAGTTCCCTTATGGCAGTTGGGTCCTGAAAGTAGGCATCTGGGAGTTCCATACATTGTTTTTCCTGGTAATGTTGGTGACAGTGGAGCATTGGCCGAGCTAGTGAAATCCTGGGCTCGTCCAATCAGATTTTCAACTAAAGAGCTTCTTCTGAACGCAGAAAAGGGTGGATATGCTGTTGGAGCATTCAATGTTTATAATTTAGAAGGAGTAGAGGCAGTTGTTGCTGCAGCAGAGGAACAACAGAGTCCTGCCATATTACAGATCCATCCCGGTGCCTTGAAGCAAGGAGGCATGCCGTTGATTGCTTGTTGCATCTCTGCTGCTGAACAAGCCAGTGTACCCATAACTGTACACTTTGATCATGGAACTTCAAAGCAAGATCTGGTGACAGCTCTTGAGTTGGGATTTGATTCTGTCATGGTAGATGGTTCACATCTTTCCTTTACAGAAAATGTTTCGTACACTAGGTTCATAACATTCTTGGCTCACTCAAAAGGCTTGCTAGTAGAAGCTGAACTAGGGAGATTGTCAGGGACAGAAGATGATTTGACTGTTGAAGATTATGAAGCAAGGCTGACCGATGTTAAGCAGGCTCAAGAGTTCATCCATGAAACTGGTATAGATGCTTTGGCAGTGTGTATCGGAAATGTCCATGGTAAATACCCTGCAAGTGGCCCAAATCTGAGACTTGACTTGCTTAAGGAATTGCACGCGTTGAGTTCAAAGAAAGGAGTCTTTCTGGTGCTTCATGGAGCTTCTGGTGTGCCCAAGGAACTCGTAAAGGGATGCATAGAGCTAGGTGTAAGAAAGTTCAATGTAAATACCGAGGTACGGAAAGCTTATATGGATTCCCTTAGCAACTCAAAGAAAGATCTGGTCCATGTTATGGAGTCAGCAAAACTAGCCATGAAAGCAGTGATTGCAGAGAAGATGATTCTATTTGGCTCAGCAGGAAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1392

Amino Acids

149.3

Weight (kDa)

5.88

Isoelectric Point (pI)

29.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_11 PF14833 185 - 306 6.8e-18 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 342 - 500 7.8e-36 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 507 - 627 9e-29 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 674 - 911 2.7e-57 Sugar-binding N-terminal domain
NBD_C PF17042 936 - 1102 1e-33 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1116 - 1391 1e-91 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 390, 3411
Acc65I GGTACC 1 cut(s) 635
AccB1I GGYRCC 3 cut(s) 635, 718, 3488
AccB7I CCANNNNNTGG 2 cut(s) 2398, 4102
AccI GTMKAC 1 cut(s) 1849
AccII CGCG 4 cut(s) 768, 1132, 2484, 3929
AciI CCGC 6 cut(s) 73, 78, 686, 1024, 2367, 2707
AclWI GGATC 9 cut(s) 1368, 1666, 1967, 2033, 2348, 2617, 2781, 2827, 3472
AcoI YGGCCR 4 cut(s) 687, 1964, 2872, 3306
AcsI RAATTY 2 cut(s) 361, 942
AcuI CTGAAG 3 cut(s) 453, 858, 1385
AfaI GTAC 8 cut(s) 353, 637, 1462, 2953, 3555, 3567, 3677, 4047
AfeI AGCGCT 1 cut(s) 1237
AflII CTTAAG 3 cut(s) 1454, 2858, 3914
AflIII ACRYGT 2 cut(s) 371, 3927
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 7 cut(s) 714, 1312, 1664, 2153, 3211, 3277, 3323
AjuI GAANNNNNNNTTGG 6 cut(s) 846, 878, 915, 947, 3328, 3360
Alw21I GWGCWC 4 cut(s) 738, 751, 1606, 2168
Alw26I GTCTC 6 cut(s) 95, 216, 959, 1877, 2810, 3895
AlwI GGATC 9 cut(s) 1368, 1666, 1967, 2033, 2348, 2617, 2781, 2827, 3472
AlwNI CAGNNNCTG 4 cut(s) 434, 1832, 2881, 2960
Aor51HI AGCGCT 1 cut(s) 1237
AoxI GGCC 7 cut(s) 687, 1964, 2175, 2567, 2872, 3306, 3889
ApoI RAATTY 2 cut(s) 361, 942
AseI ATTAAT 2 cut(s) 273, 2186
Asp718I GGTACC 1 cut(s) 635
AspLEI GCGC 2 cut(s) 1238, 1726
AspS9I GGNCC 4 cut(s) 2567, 3236, 3890, 4093
AsuC2I CCSGG 1 cut(s) 3486
AsuHPI GGTGA 8 cut(s) 557, 842, 1030, 1994, 2276, 2336, 3302, 3613
AsuII TTCGAA 2 cut(s) 1777, 2474
AsuNHI GCTAGC 2 cut(s) 1555, 2633
AvaII GGWCC 2 cut(s) 3236, 4093
BaeGI GKGCMC 2 cut(s) 723, 3984
BalI TGGCCA 2 cut(s) 1966, 2874
BanI GGYRCC 3 cut(s) 635, 718, 3488
BanII GRGCYC 3 cut(s) 1606, 2168, 3331
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 4 cut(s) 738, 751, 1606, 2168
BbvCI CCTCAGC 1 cut(s) 1584
BccI CCATC 9 cut(s) 568, 872, 1282, 1534, 2323, 2387, 2959, 3489, 3635
BceAI ACGGC 1 cut(s) 3496
BciT130I CCWGG 7 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325
BclI TGATCA 4 cut(s) 892, 2410, 2670, 3574
BcnI CCSGG 1 cut(s) 3486
BcoDI GTCTC 6 cut(s) 95, 216, 959, 1877, 2810, 3895
BfmI CTRYAG 6 cut(s) 1836, 2217, 2250, 2424, 2925, 3441
BfoI RGCGCY 1 cut(s) 1239
BfrI CTTAAG 3 cut(s) 1454, 2858, 3914
BglI GCCNNNNNGGC 1 cut(s) 425
BglII AGATCT 2 cut(s) 3595, 4087
BlpI GCTNAGC 1 cut(s) 4162
BmcAI AGTACT 1 cut(s) 1462
Bme1390I CCNGG 8 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325, 3486
Bme18I GGWCC 2 cut(s) 3236, 4093
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 4 cut(s) 2567, 3236, 3890, 4093
BmiI GGNNCC 5 cut(s) 637, 720, 1785, 3237, 3490
BmrFI CCNGG 8 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325, 3486
BmtI GCTAGC 2 cut(s) 1559, 2637
BpiI GAAGAC 1 cut(s) 864
BpmI CTGGAG 5 cut(s) 1152, 1299, 1581, 2137, 3234
Bpu10I CCTNAGC 3 cut(s) 978, 1584, 4070
Bpu1102I GCTNAGC 1 cut(s) 4162
Bpu14I TTCGAA 2 cut(s) 1777, 2474
BpuEI CTTGAG 5 cut(s) 64, 611, 1209, 3632, 3795
BpuMI CCSGG 1 cut(s) 3486
BsaBI GATNNNNATC 2 cut(s) 2037, 2409
BsaI GGTCTC 1 cut(s) 2810
BsaJI CCNNGG 7 cut(s) 656, 2154, 2769, 3324, 3868, 3984, 4041
BsaXI ACNNNNNCTCC 2 cut(s) 1294, 1324
Bse118I RCCGGY 2 cut(s) 1406, 3029
Bse1I ACTGG 7 cut(s) 503, 1468, 2050, 3012, 3087, 3549, 3835
Bse3DI GCAATG 6 cut(s) 522, 1056, 1417, 1596, 2166, 2922
Bse8I GATNNNNATC 2 cut(s) 2037, 2409
BseBI CCWGG 7 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325
BseDI CCNNGG 7 cut(s) 656, 2154, 2769, 3324, 3868, 3984, 4041
BseJI GATNNNNATC 2 cut(s) 2037, 2409
BseMI GCAATG 6 cut(s) 522, 1056, 1417, 1596, 2166, 2922
BseNI ACTGG 7 cut(s) 503, 1468, 2050, 3012, 3087, 3549, 3835
BseRI GAGGAG 1 cut(s) 2510
BseSI GKGCMC 2 cut(s) 723, 3984
BseX3I CGGCCG 1 cut(s) 687
BseYI CCCAGC 3 cut(s) 723, 1842, 1954
BsgI GTGCAG 2 cut(s) 2185, 3063
Bsh1236I CGCG 4 cut(s) 768, 1132, 2484, 3929
Bsh1285I CGRYCG 1 cut(s) 690
BshFI GGCC 7 cut(s) 689, 1966, 2177, 2569, 2874, 3308, 3891
BshNI GGYRCC 3 cut(s) 635, 718, 3488
BsiEI CGRYCG 1 cut(s) 690
BsiHKAI GWGCWC 4 cut(s) 738, 751, 1606, 2168
BsiSI CCGG 5 cut(s) 690, 1407, 1919, 3030, 3486
BslFI GGGAC 5 cut(s) 196, 1429, 3024, 3213, 3763
BsmAI GTCTC 6 cut(s) 95, 216, 959, 1877, 2810, 3895
BsmFI GGGAC 5 cut(s) 196, 1429, 3024, 3213, 3763
BsmI GAATGC 1 cut(s) 3398
BsnI GGCC 7 cut(s) 689, 1966, 2177, 2569, 2874, 3308, 3891
Bso31I GGTCTC 1 cut(s) 2810
Bsp119I TTCGAA 2 cut(s) 1777, 2474
Bsp1286I GDGCHC 7 cut(s) 723, 738, 751, 1606, 2168, 3331, 3984
Bsp1407I TGTACA 2 cut(s) 351, 3565
Bsp1720I GCTNAGC 1 cut(s) 4162
Bsp19I CCATGG 1 cut(s) 3868
Bsp68I TCGCGA 1 cut(s) 2484
BspACI CCGC 6 cut(s) 73, 78, 686, 1024, 2367, 2707
BspANI GGCC 7 cut(s) 689, 1966, 2177, 2569, 2874, 3308, 3891
BspFNI CGCG 4 cut(s) 768, 1132, 2484, 3929
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 5 cut(s) 637, 720, 1785, 3237, 3490
BspMAI CTGCAG 5 cut(s) 1840, 2221, 2428, 2929, 3445
BspOI GCTAGC 2 cut(s) 1559, 2637
BspPI GGATC 9 cut(s) 1368, 1666, 1967, 2033, 2348, 2617, 2781, 2827, 3472
BspQI GCTCTTC 1 cut(s) 2847
BspT104I TTCGAA 2 cut(s) 1777, 2474
BspT107I GGYRCC 3 cut(s) 635, 718, 3488
BspTI CTTAAG 3 cut(s) 1454, 2858, 3914
BspTNI GGTCTC 1 cut(s) 2810
BsrDI GCAATG 6 cut(s) 522, 1056, 1417, 1596, 2166, 2922
BsrFI RCCGGY 2 cut(s) 1406, 3029
BsrGI TGTACA 2 cut(s) 351, 3565
BsrI ACTGG 7 cut(s) 503, 1468, 2050, 3012, 3087, 3549, 3835
BssAI RCCGGY 2 cut(s) 1406, 3029
BssECI CCNNGG 7 cut(s) 656, 2154, 2769, 3324, 3868, 3984, 4041
BssT1I CCWWGG 3 cut(s) 2769, 3868, 3984
Bst2UI CCWGG 7 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325
Bst4CI ACNGT 8 cut(s) 319, 1306, 2059, 2092, 2251, 3296, 3565, 3769
Bst6I CTCTTC 3 cut(s) 219, 2847, 2925
BstAFI CTTAAG 3 cut(s) 1454, 2858, 3914
BstAPI GCANNNNNTGC 4 cut(s) 959, 3185, 3437, 4136
BstAUI TGTACA 2 cut(s) 351, 3565
BstBI TTCGAA 2 cut(s) 1777, 2474
BstDSI CCRYGG 2 cut(s) 656, 3868
BstENI CCTNNNNNAGG 1 cut(s) 3131
BstFNI CGCG 4 cut(s) 768, 1132, 2484, 3929
BstH2I RGCGCY 1 cut(s) 1239
BstHHI GCGC 2 cut(s) 1238, 1726
BstMAI GTCTC 6 cut(s) 95, 216, 959, 1877, 2810, 3895
BstMCI CGRYCG 1 cut(s) 690
BstNI CCWGG 7 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325
BstNSI RCATGY 3 cut(s) 1696, 2619, 3511
BstSCI CCNGG 8 cut(s) 714, 1312, 1664, 2153, 3211, 3277, 3323, 3484
BstSFI CTRYAG 6 cut(s) 1836, 2217, 2250, 2424, 2925, 3441
BstSLI GKGCMC 2 cut(s) 723, 3984
BstUI CGCG 4 cut(s) 768, 1132, 2484, 3929
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 6 cut(s) 1360, 1972, 2773, 3477, 3595, 4087
BstXI CCANNNNNNTGG 2 cut(s) 1962, 3830
BstYI RGATCY 6 cut(s) 1360, 1972, 2773, 3477, 3595, 4087
BstZI CGGCCG 1 cut(s) 687
BsuRI GGCC 7 cut(s) 689, 1966, 2177, 2569, 2874, 3308, 3891
BtgI CCRYGG 2 cut(s) 656, 3868
BtrI CACGTC 1 cut(s) 372
BtsI GCAGTG 4 cut(s) 2533, 2709, 3855, 4137
BtsIMutI CAGTG 9 cut(s) 185, 1302, 2533, 2709, 3080, 3301, 3556, 3855, 4137
BtuMI TCGCGA 1 cut(s) 2484
CaiI CAGNNNCTG 4 cut(s) 434, 1832, 2881, 2960
CciI TCATGA 1 cut(s) 456
CfoI GCGC 2 cut(s) 1238, 1726
Cfr10I RCCGGY 2 cut(s) 1406, 3029
Cfr13I GGNCC 4 cut(s) 2567, 3236, 3890, 4093
CseI GACGC 1 cut(s) 2246
CsiI ACCWGGT 1 cut(s) 714
Csp6I GTAC 8 cut(s) 352, 636, 1461, 2952, 3554, 3566, 3676, 4046
CspCI CAANNNNNGTGG 2 cut(s) 1129, 1164
CviQI GTAC 8 cut(s) 352, 636, 1461, 2952, 3554, 3566, 3676, 4046
DraI TTTAAA 4 cut(s) 132, 307, 444, 492
EaeI YGGCCR 4 cut(s) 687, 1964, 2872, 3306
EagI CGGCCG 1 cut(s) 687
Eam1104I CTCTTC 3 cut(s) 219, 2847, 2925
EarI CTCTTC 3 cut(s) 219, 2847, 2925
Ecl136II GAGCTC 2 cut(s) 1604, 2166
EclXI CGGCCG 1 cut(s) 687
Eco130I CCWWGG 3 cut(s) 2769, 3868, 3984
Eco24I GRGCYC 3 cut(s) 1606, 2168, 3331
Eco31I GGTCTC 1 cut(s) 2810
Eco32I GATATC 3 cut(s) 673, 1732, 2389
Eco47I GGWCC 2 cut(s) 3236, 4093
Eco47III AGCGCT 1 cut(s) 1237
Eco52I CGGCCG 1 cut(s) 687
Eco53kI GAGCTC 2 cut(s) 1604, 2166
Eco57I CTGAAG 3 cut(s) 453, 858, 1385
EcoICRI GAGCTC 2 cut(s) 1604, 2166
EcoNI CCTNNNNNAGG 1 cut(s) 3131
EcoO109I RGGNCCY 1 cut(s) 3236
EcoRII CCWGG 7 cut(s) 714, 1312, 1664, 2153, 3211, 3277, 3323
EcoRV GATATC 3 cut(s) 673, 1732, 2389
EcoT14I CCWWGG 3 cut(s) 2769, 3868, 3984
EcoT22I ATGCAT 3 cut(s) 2338, 2621, 4007
EcoT38I GRGCYC 3 cut(s) 1606, 2168, 3331
ErhI CCWWGG 3 cut(s) 2769, 3868, 3984
FalI AAGNNNNNCTT 8 cut(s) 1899, 1931, 1919, 1951, 2684, 2716, 3345, 3377
FaqI GGGAC 5 cut(s) 196, 1429, 3024, 3213, 3763
FauI CCCGC 1 cut(s) 2360
FauNDI CATATG 2 cut(s) 106, 2824
FbaI TGATCA 4 cut(s) 892, 2410, 2670, 3574
FblI GTMKAC 1 cut(s) 1849
FriOI GRGCYC 3 cut(s) 1606, 2168, 3331
GlaI GCGC 2 cut(s) 1237, 1725
GsaI CCCAGC 3 cut(s) 727, 1846, 1958
GsuI CTGGAG 5 cut(s) 1152, 1299, 1581, 2137, 3234
HaeII RGCGCY 1 cut(s) 1239
HaeIII GGCC 7 cut(s) 689, 1966, 2177, 2569, 2874, 3308, 3891
HapII CCGG 5 cut(s) 690, 1407, 1919, 3030, 3486
HgaI GACGC 1 cut(s) 2246
HhaI GCGC 2 cut(s) 1238, 1726
Hin6I GCGC 2 cut(s) 1236, 1724
HinP1I GCGC 2 cut(s) 1236, 1724
HincII GTYRAC 2 cut(s) 252, 1850
HindII GTYRAC 2 cut(s) 252, 1850
HindIII AAGCTT 2 cut(s) 527, 4053
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 5 cut(s) 690, 1407, 1919, 3030, 3486
HphI GGTGA 8 cut(s) 557, 842, 1030, 1994, 2276, 2336, 3302, 3613
Hpy166II GTNNAC 6 cut(s) 252, 1850, 3554, 3568, 3647, 3678
Hpy8I GTNNAC 6 cut(s) 252, 1850, 3554, 3568, 3647, 3678
HpyAV CCTTC 6 cut(s) 418, 1346, 1901, 2555, 2599, 3413
HpyCH4III ACNGT 8 cut(s) 319, 1306, 2059, 2092, 2251, 3296, 3565, 3769
HpyCH4IV ACGT 2 cut(s) 284, 371
HpySE526I ACGT 2 cut(s) 284, 371
HspAI GCGC 2 cut(s) 1236, 1724
KpnI GGTACC 1 cut(s) 639
KroI GCCGGC 1 cut(s) 1406
KroNI GCCGGC 1 cut(s) 1408
Ksp22I TGATCA 4 cut(s) 892, 2410, 2670, 3574
KspAI GTTAAC 1 cut(s) 252
LguI GCTCTTC 1 cut(s) 2847
MabI ACCWGGT 1 cut(s) 714
MaeII ACGT 2 cut(s) 284, 371
MfeI CAATTG 2 cut(s) 983, 1550
MflI RGATCY 6 cut(s) 1360, 1972, 2773, 3477, 3595, 4087
MhlI GDGCHC 7 cut(s) 723, 738, 751, 1606, 2168, 3331, 3984
MlsI TGGCCA 2 cut(s) 1966, 2874
MluI ACGCGT 1 cut(s) 3927
MluNI TGGCCA 2 cut(s) 1966, 2874
MlyI GAGTC 3 cut(s) 3469, 3957, 4115
MmeI TCCRAC 3 cut(s) 2066, 2797, 3373
Mox20I TGGCCA 2 cut(s) 1966, 2874
Mph1103I ATGCAT 3 cut(s) 2338, 2621, 4007
MroNI GCCGGC 1 cut(s) 1406
MscI TGGCCA 2 cut(s) 1966, 2874
MslI CAYNNNNRTG 1 cut(s) 3486
Msp20I TGGCCA 2 cut(s) 1966, 2874
MspA1I CMGCKG 7 cut(s) 78, 383, 727, 1958, 2507, 2663, 2960
MspCI CTTAAG 3 cut(s) 1454, 2858, 3914
MspI CCGG 5 cut(s) 690, 1407, 1919, 3030, 3486
MspR9I CCNGG 8 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325, 3486
MunI CAATTG 2 cut(s) 983, 1550
Mva1269I GAATGC 1 cut(s) 3398
MvaI CCWGG 7 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325
MvnI CGCG 4 cut(s) 768, 1132, 2484, 3929
NaeI GCCGGC 1 cut(s) 1408
NciI CCSGG 1 cut(s) 3486
NcoI CCATGG 1 cut(s) 3868
NdeI CATATG 2 cut(s) 106, 2824
NgoMIV GCCGGC 1 cut(s) 1406
NheI GCTAGC 2 cut(s) 1555, 2633
NlaIV GGNNCC 5 cut(s) 637, 720, 1785, 3237, 3490
NmeAIII GCCGAG 1 cut(s) 3334
NmuCI GTSAC 3 cut(s) 1210, 3290, 3601
NruI TCGCGA 1 cut(s) 2484
NsiI ATGCAT 3 cut(s) 2338, 2621, 4007
NspI RCATGY 3 cut(s) 1696, 2619, 3511
NspV TTCGAA 2 cut(s) 1777, 2474
PaeI GCATGC 1 cut(s) 3511
PagI TCATGA 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 2847
PctI GAATGC 1 cut(s) 3398
PdiI GCCGGC 1 cut(s) 1408
PflMI CCANNNNNTGG 2 cut(s) 2398, 4102
PfoI TCCNGGA 2 cut(s) 1312, 1664
PleI GAGTC 3 cut(s) 3468, 3956, 4114
PpsI GAGTC 3 cut(s) 3468, 3956, 4114
PpuMI RGGWCCY 1 cut(s) 3236
PshBI ATTAAT 2 cut(s) 273, 2186
PsiI TTATAA 2 cut(s) 390, 3411
Psp124BI GAGCTC 2 cut(s) 1606, 2168
Psp5II RGGWCCY 1 cut(s) 3236
Psp6I CCWGG 7 cut(s) 714, 1312, 1664, 2153, 3211, 3277, 3323
PspFI CCCAGC 3 cut(s) 723, 1842, 1954
PspGI CCWGG 7 cut(s) 714, 1312, 1664, 2153, 3211, 3277, 3323
PspN4I GGNNCC 5 cut(s) 637, 720, 1785, 3237, 3490
PspPI GGNCC 4 cut(s) 2567, 3236, 3890, 4093
PspPPI RGGWCCY 1 cut(s) 3236
PsrI GAACNNNNNNTAC 2 cut(s) 3668, 3700
PstI CTGCAG 5 cut(s) 1840, 2221, 2428, 2929, 3445
PstNI CAGNNNCTG 4 cut(s) 434, 1832, 2881, 2960
PsuI RGATCY 6 cut(s) 1360, 1972, 2773, 3477, 3595, 4087
PvuII CAGCTG 6 cut(s) 383, 727, 1958, 2507, 2663, 2960
RruI TCGCGA 1 cut(s) 2484
RsaI GTAC 8 cut(s) 353, 637, 1462, 2953, 3555, 3567, 3677, 4047
RsaNI GTAC 8 cut(s) 352, 636, 1461, 2952, 3554, 3566, 3676, 4046
RseI CAYNNNNRTG 1 cut(s) 3486
SacI GAGCTC 2 cut(s) 1606, 2168
SalI GTCGAC 1 cut(s) 1848
SapI GCTCTTC 1 cut(s) 2847
Sau96I GGNCC 4 cut(s) 2567, 3236, 3890, 4093
ScaI AGTACT 1 cut(s) 1462
SchI GAGTC 3 cut(s) 3469, 3957, 4115
ScrFI CCNGG 8 cut(s) 716, 1314, 1666, 2155, 3213, 3279, 3325, 3486
SduI GDGCHC 7 cut(s) 723, 738, 751, 1606, 2168, 3331, 3984
SexAI ACCWGGT 1 cut(s) 714
SfcI CTRYAG 6 cut(s) 1836, 2217, 2250, 2424, 2925, 3441
SfuI TTCGAA 2 cut(s) 1777, 2474
SinI GGWCC 2 cut(s) 3236, 4093
SmiMI CAYNNNNRTG 1 cut(s) 3486
SmlI CTYRAG 8 cut(s) 43, 590, 1224, 1454, 2858, 3611, 3810, 3914
SmoI CTYRAG 8 cut(s) 43, 590, 1224, 1454, 2858, 3611, 3810, 3914
SphI GCATGC 1 cut(s) 3511
SsiI CCGC 6 cut(s) 73, 78, 686, 1024, 2367, 2707
SstI GAGCTC 2 cut(s) 1606, 2168
StyD4I CCNGG 8 cut(s) 714, 1312, 1664, 2153, 3211, 3277, 3323, 3484
StyI CCWWGG 3 cut(s) 2769, 3868, 3984
TaaI ACNGT 8 cut(s) 319, 1306, 2059, 2092, 2251, 3296, 3565, 3769
TaiI ACGT 2 cut(s) 287, 374
TaqI TCGA 5 cut(s) 1777, 1799, 1849, 2270, 2474
TaqII GACCGA 1 cut(s) 3809
TatI WGTACW 4 cut(s) 351, 1460, 2951, 3565
TauI GCSGC 2 cut(s) 689, 2709
TscAI CASTG 9 cut(s) 192, 1309, 2533, 2716, 3087, 3301, 3556, 3855, 4137
TseFI GTSAC 3 cut(s) 1210, 3290, 3601
Tsp45I GTSAC 3 cut(s) 1210, 3290, 3601
TspGWI ACGGA 3 cut(s) 645, 2809, 4063
TspRI CASTG 9 cut(s) 192, 1309, 2533, 2716, 3087, 3301, 3556, 3855, 4137
Van91I CCANNNNNTGG 2 cut(s) 2398, 4102
Vha464I CTTAAG 3 cut(s) 1454, 2858, 3914
VpaK11BI GGWCC 2 cut(s) 3236, 4093
VspI ATTAAT 2 cut(s) 273, 2186
XagI CCTNNNNNAGG 1 cut(s) 3131
XapI RAATTY 2 cut(s) 361, 942
XceI RCATGY 3 cut(s) 1696, 2619, 3511
XmiI GTMKAC 1 cut(s) 1849
ZrmI AGTACT 1 cut(s) 1462
Zsp2I ATGCAT 3 cut(s) 2338, 2621, 4007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.