AT1G20260

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007224
Physical Location & Seq
Forward (+)
7016599 .. 7020690
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20260.1

Sequence Viewer

Length: 1464 bp
ATGGTGGAGACTAGTATCGACATGGAGGAAGGAACTCTCGAGATCGGAATGGAGTATAGAACTGTCTCCGGAGTTGCTGGACCTTTGGTCATCCTTGACAAAGTGAAGGGTCCAAAGTACCAGGAGATTGTTAATATTCGCTTAGGAGATGGATCAACGAGACGTGGTCAGGTCTTGGAGGTTGATGGGGAGAAAGCTGTTGTCCAGGTTTTTGAAGGAACATCTGGGATTGACAACAAGTTTACCACCGTGCAATTCACAGGAGAGGTACTGAAAACGCCTGTGTCATTGGACATGCTTGGCCGCATATTTAACGGCTCGGGAAAGCCAATTGATAATGGCCCTCCTATTTTGCCAGAGGCATACCTTGATATTTCTGGAAGTTCAATCAACCCCAGTGAAAGAACCTACCCTGAAGAGATGATACAAACGGGGATTTCTACCATTGATGTCATGAACTCCATTGCTCGTGGACAGAAGATTCCTCTTTTCTCTGCTGCTGGTCTACCTCATAATGAGATTGCTGCTCAAATTTGTCGTCAGGCTGGACTTGTGAAGCGGTTGGAAAAGACTGAGAACTTAATTCAGGAGGATCATGGAGAGGACAATTTTGCAATTGTTTTTGCAGCTATGGGTGTAAACATGGAGACAGCTCAGTTTTTCAAGCGTGATTTTGAAGAAAATGGATCGATGGAAAGGGTTACCCTTTTCCTGAACTTGGCCAATGATCCAACCATTGAGAGAATTATCACTCCTCGAATTGCCCTAACAACCGCTGAATATTTGGCTTATGAATGTGGAAAGCATGTGCTTGTTATATTGACAGACATGAGTTCTTATGCAGATGCTCTTCGTGAGGTTTCTGCTGCTCGAGAAGAAGTTCCTGGAAGACGTGGATATCCGGGGTATATGTATACAGATCTTGCAACTATTTATGAACGCGCGGGACGTATTGAAGGAAGAAAGGGTTCCATTACCCAAATCCCTATCCTGACTATGCCCAATGACGATATCACTCACCCTACTCCGGATCTTACTGGTTACATTACTGAGGGTCAGATATATATTGATAGGCAACTTCACAACAGACAGATATACCCACCCATTAACGTGCTTCCATCACTTTCTCGGCTGATGAAGAGTGCTATTGGTGAAGGAATGACTCGCAAAGACCATTCTGATGTGTCCAACCAGCTATACGCAAATTATGCAATCGGAAAAGATGTTCAAGCCATGAAAGCTGTTGTTGGAGAAGAAGCTCTGTCTTCCGAGGATCTGCTGTATTTAGAGTTTCTGGATAAATTTGAAAGGAAGTTTGTGATGCAAGGCGCATACGATACAAGGAACATCTTCCAGTCGCTTGACTTGGCATGGACACTGCTCCGTATCTTCCCACGTGAGCTTCTTCACCGTATTCCTGCCAAAACACTTGATCAATTCTACAGCCGCGACTCCACAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001678 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0008064 GO:0008092 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010182 GO:0010255 GO:0010468 GO:0010556 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0019219 GO:0019222 GO:0019725 GO:0022607 GO:0023052 GO:0030029 GO:0030036 GO:0030054 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031090 GO:0031323 GO:0031326 GO:0031333 GO:0032271 GO:0032272 GO:0032535 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033500 GO:0034284 GO:0042221 GO:0042592 GO:0042593 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043242 GO:0043244 GO:0043254 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046686 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051493 GO:0051494 GO:0051693 GO:0051716 GO:0055044 GO:0055082 GO:0060255 GO:0061572 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0097435 GO:0098588 GO:0098805 GO:0110053 GO:1901700 GO:1901701 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N

Protein Analysis

487

Amino Acids

54.31

Weight (kDa)

4.99

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 22 - 88 1.8e-15 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 145 - 376 1.5e-75 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 382 - 481 3.3e-34 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 505, 914
AccII CGCG 3 cut(s) 942, 944, 1449
AccIII TCCGGA 2 cut(s) 68, 1027
AciI CCGC 5 cut(s) 304, 559, 774, 944, 1447
AclWI GGATC 6 cut(s) 160, 600, 694, 722, 1038, 1281
AcoI YGGCCR 2 cut(s) 301, 720
AcsI RAATTY 2 cut(s) 531, 1301
AcuI CTGAAG 1 cut(s) 435
AcvI CACGTG 1 cut(s) 1397
AfaI GTAC 2 cut(s) 119, 270
AfiI CCNNNNNNNGG 2 cut(s) 718, 1027
AgsI TTSAA 7 cut(s) 215, 387, 664, 677, 956, 1229, 1307
AhdI GACNNNNNGTC 1 cut(s) 86
AhlI ACTAGT 1 cut(s) 11
AjiI CACGTC 2 cut(s) 164, 893
AjnI CCWGG 3 cut(s) 120, 204, 883
AloI GAACNNNNNNTCC 2 cut(s) 1209, 1241
AluBI AGCT 8 cut(s) 197, 629, 653, 1195, 1241, 1259, 1402, 1461
AluI AGCT 8 cut(s) 197, 629, 653, 1195, 1241, 1259, 1402, 1461
Alw26I GTCTC 4 cut(s) 2, 70, 154, 641
AlwI GGATC 6 cut(s) 160, 600, 694, 722, 1038, 1281
Ama87I CYCGRG 3 cut(s) 38, 319, 870
Aor13HI TCCGGA 2 cut(s) 68, 1027
AoxI GGCC 3 cut(s) 301, 340, 720
ApeKI GCWGC 4 cut(s) 497, 524, 626, 866
ApoI RAATTY 2 cut(s) 531, 1301
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
Asp700I GAANNNNTTC 3 cut(s) 879, 967, 1349
AspLEI GCGC 2 cut(s) 944, 1331
AspS9I GGNCC 3 cut(s) 80, 110, 341
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 3 cut(s) 1010, 1163, 1400
AvaI CYCGRG 3 cut(s) 38, 319, 870
AvaII GGWCC 2 cut(s) 80, 110
BalI TGGCCA 1 cut(s) 722
BarI GAAGNNNNNNTAC 2 cut(s) 408, 440
BauI CACGAG 1 cut(s) 468
BbrPI CACGTG 1 cut(s) 1397
BbsI GAAGAC 2 cut(s) 895, 1257
BbvI GCAGC 4 cut(s) 484, 511, 638, 853
BccI CCATC 4 cut(s) 143, 179, 685, 1126
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 3 cut(s) 122, 206, 885
BclI TGATCA 1 cut(s) 1432
BcnI CCSGG 1 cut(s) 903
BcoDI GTCTC 4 cut(s) 2, 70, 154, 641
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 1441
BglII AGATCT 1 cut(s) 919
BisI GCNGC 6 cut(s) 304, 498, 525, 627, 867, 1447
BlsI GCNGC 6 cut(s) 305, 499, 526, 628, 868, 1448
Bme1390I CCNGG 4 cut(s) 122, 206, 885, 903
Bme18I GGWCC 2 cut(s) 80, 110
BmeRI GACNNNNNGTC 1 cut(s) 86
BmeT110I CYCGRG 3 cut(s) 38, 319, 870
BmgBI CACGTC 2 cut(s) 164, 893
BmgT120I GGNCC 3 cut(s) 80, 110, 341
BmiI GGNNCC 2 cut(s) 111, 970
BmrFI CCNGG 4 cut(s) 122, 206, 885, 903
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 2 cut(s) 835, 1311
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 895, 1257
Bpu10I CCTNAGC 1 cut(s) 142
BpuMI CCSGG 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 689
BsaAI YACGTR 1 cut(s) 1397
BsaJI CCNNGG 2 cut(s) 902, 1269
BsaWI WCCGGW 2 cut(s) 68, 1027
Bsc4I CCNNNNNNNGG 2 cut(s) 718, 1027
Bse1I ACTGG 3 cut(s) 396, 1042, 1354
Bse3DI GCAATG 1 cut(s) 462
BseAI TCCGGA 2 cut(s) 68, 1027
BseBI CCWGG 3 cut(s) 122, 206, 885
BseCI ATCGAT 1 cut(s) 689
BseDI CCNNGG 2 cut(s) 902, 1269
BseGI GGATG 1 cut(s) 90
BseLI CCNNNNNNNGG 2 cut(s) 718, 1027
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 3 cut(s) 564, 668, 1041
BseNI ACTGG 3 cut(s) 396, 1042, 1354
BseRI GAGGAG 1 cut(s) 744
BseXI GCAGC 4 cut(s) 484, 511, 638, 853
Bsh1236I CGCG 3 cut(s) 942, 944, 1449
BshFI GGCC 3 cut(s) 303, 342, 722
BshVI ATCGAT 1 cut(s) 689
BsiHKCI CYCGRG 3 cut(s) 38, 319, 870
BsiSI CCGG 3 cut(s) 69, 902, 1028
BslFI GGGAC 1 cut(s) 960
BslI CCNNNNNNNGG 2 cut(s) 718, 1027
BsmAI GTCTC 4 cut(s) 2, 70, 154, 641
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 960
BsnI GGCC 3 cut(s) 303, 342, 722
BsoBI CYCGRG 3 cut(s) 38, 319, 870
Bsp13I TCCGGA 2 cut(s) 68, 1027
Bsp143I GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
BspACI CCGC 5 cut(s) 304, 559, 774, 944, 1447
BspANI GGCC 3 cut(s) 303, 342, 722
BspCNI CTCAG 3 cut(s) 565, 667, 1042
BspDI ATCGAT 1 cut(s) 689
BspEI TCCGGA 2 cut(s) 68, 1027
BspFNI CGCG 3 cut(s) 942, 944, 1449
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 2 cut(s) 111, 970
BspPI GGATC 6 cut(s) 160, 600, 694, 722, 1038, 1281
BspQI GCTCTTC 1 cut(s) 855
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 3 cut(s) 396, 1042, 1354
BssECI CCNNGG 2 cut(s) 902, 1269
BssMI GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
BssNAI GTATAC 1 cut(s) 915
BssSI CACGAG 1 cut(s) 468
Bst1107I GTATAC 1 cut(s) 915
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 3 cut(s) 122, 206, 885
Bst4CI ACNGT 3 cut(s) 64, 250, 1412
Bst6I CTCTTC 3 cut(s) 411, 855, 1133
BstAPI GCANNNNNTGC 1 cut(s) 1208
BstBAI YACGTR 1 cut(s) 1397
BstDEI CTNAG 4 cut(s) 142, 573, 654, 1050
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 1 cut(s) 90
BstFNI CGCG 3 cut(s) 942, 944, 1449
BstHHI GCGC 2 cut(s) 944, 1331
BstKTI GATC 9 cut(s) 45, 155, 595, 689, 730, 922, 1033, 1276, 1435
BstMAI GTCTC 4 cut(s) 2, 70, 154, 641
BstMBI GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
BstMWI GCNNNNNNNGC 2 cut(s) 1208, 1238
BstNI CCWGG 3 cut(s) 122, 206, 885
BstNSI RCATGY 2 cut(s) 298, 809
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 4 cut(s) 120, 204, 883, 901
BstSFI CTRYAG 1 cut(s) 1441
BstUI CGCG 3 cut(s) 942, 944, 1449
BstV1I GCAGC 4 cut(s) 484, 511, 638, 853
BstV2I GAAGAC 2 cut(s) 895, 1257
BstX2I RGATCY 3 cut(s) 919, 1030, 1273
BstYI RGATCY 3 cut(s) 919, 1030, 1273
BstZ17I GTATAC 1 cut(s) 915
Bsu15I ATCGAT 1 cut(s) 689
BsuRI GGCC 3 cut(s) 303, 342, 722
BsuTUI ATCGAT 1 cut(s) 689
BtrI CACGTC 2 cut(s) 164, 893
BtsCI GGATG 1 cut(s) 90
BtsI GCAGTG 1 cut(s) 1376
BtsIMutI CAGTG 2 cut(s) 403, 1376
CciI TCATGA 1 cut(s) 453
CfoI GCGC 2 cut(s) 944, 1331
Cfr13I GGNCC 3 cut(s) 80, 110, 341
ClaI ATCGAT 1 cut(s) 689
Csp6I GTAC 2 cut(s) 118, 269
CviAII CATG 9 cut(s) 22, 295, 454, 596, 643, 806, 829, 1234, 1371
CviQI GTAC 2 cut(s) 118, 269
DdeI CTNAG 4 cut(s) 142, 573, 654, 1050
DpnI GATC 9 cut(s) 44, 154, 594, 688, 729, 921, 1032, 1275, 1434
DpnII GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
DriI GACNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 301, 720
Eam1104I CTCTTC 3 cut(s) 411, 855, 1133
Eam1105I GACNNNNNGTC 1 cut(s) 86
EarI CTCTTC 3 cut(s) 411, 855, 1133
Eco32I GATATC 2 cut(s) 899, 1012
Eco47I GGWCC 2 cut(s) 80, 110
Eco57I CTGAAG 1 cut(s) 435
Eco72I CACGTG 1 cut(s) 1397
Eco88I CYCGRG 3 cut(s) 38, 319, 870
Eco91I GGTNACC 1 cut(s) 700
EcoO65I GGTNACC 1 cut(s) 700
EcoRII CCWGG 3 cut(s) 120, 204, 883
EcoRV GATATC 2 cut(s) 899, 1012
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 9 cut(s) 25, 298, 457, 599, 646, 809, 832, 1237, 1374
FaqI GGGAC 1 cut(s) 960
FatI CATG 9 cut(s) 21, 294, 453, 595, 642, 805, 828, 1233, 1370
FauI CCCGC 1 cut(s) 937
FbaI TGATCA 1 cut(s) 1432
FblI GTMKAC 2 cut(s) 505, 914
Fnu4HI GCNGC 6 cut(s) 304, 498, 525, 627, 867, 1447
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 6 cut(s) 304, 498, 525, 627, 867, 1447
FspBI CTAG 1 cut(s) 12
GlaI GCGC 2 cut(s) 943, 1330
GluI GCNGC 6 cut(s) 304, 498, 525, 627, 867, 1447
HaeIII GGCC 3 cut(s) 303, 342, 722
HapII CCGG 3 cut(s) 69, 902, 1028
HhaI GCGC 2 cut(s) 944, 1331
Hin1II CATG 9 cut(s) 25, 298, 457, 599, 646, 809, 832, 1237, 1374
Hin6I GCGC 2 cut(s) 942, 1329
HinP1I GCGC 2 cut(s) 942, 1329
HinfI GANTC 3 cut(s) 481, 1162, 1451
HpaII CCGG 3 cut(s) 69, 902, 1028
HphI GGTGA 3 cut(s) 1010, 1163, 1400
Hpy166II GTNNAC 5 cut(s) 243, 473, 506, 640, 915
Hpy188I TCNGA 5 cut(s) 47, 1059, 1180, 1217, 1270
Hpy8I GTNNAC 5 cut(s) 243, 473, 506, 640, 915
HpyAV CCTTC 5 cut(s) 23, 100, 209, 950, 1148
HpyCH4III ACNGT 3 cut(s) 64, 250, 1412
HpyCH4IV ACGT 5 cut(s) 163, 892, 949, 1110, 1396
HpyCH4V TGCA 7 cut(s) 253, 614, 626, 842, 926, 1211, 1324
HpyF10VI GCNNNNNNNGC 2 cut(s) 1208, 1238
HpyF3I CTNAG 4 cut(s) 142, 573, 654, 1050
HpySE526I ACGT 5 cut(s) 163, 892, 949, 1110, 1396
Hsp92II CATG 9 cut(s) 25, 298, 457, 599, 646, 809, 832, 1237, 1374
HspAI GCGC 2 cut(s) 942, 1329
Kpn2I TCCGGA 2 cut(s) 68, 1027
Ksp22I TGATCA 1 cut(s) 1432
Kzo9I GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 1 cut(s) 1386
Lsp1109I GCAGC 4 cut(s) 484, 511, 638, 853
LweI GCATC 2 cut(s) 835, 1311
MaeI CTAG 1 cut(s) 12
MaeII ACGT 5 cut(s) 163, 892, 949, 1110, 1396
MaeIII GTNAC 2 cut(s) 700, 1040
MalI GATC 9 cut(s) 44, 154, 594, 688, 729, 921, 1032, 1275, 1434
MboI GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
MfeI CAATTG 2 cut(s) 330, 615
MflI RGATCY 3 cut(s) 919, 1030, 1273
MlsI TGGCCA 1 cut(s) 722
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 2 cut(s) 1156, 1445
MmeI TCCRAC 4 cut(s) 543, 755, 1212, 1228
Mox20I TGGCCA 1 cut(s) 722
MroI TCCGGA 2 cut(s) 68, 1027
MroXI GAANNNNTTC 3 cut(s) 879, 967, 1349
MscI TGGCCA 1 cut(s) 722
MseI TTAA 4 cut(s) 132, 312, 581, 1107
MslI CAYNNNNRTG 2 cut(s) 1109, 1179
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 3 cut(s) 69, 902, 1028
MspR9I CCNGG 4 cut(s) 122, 206, 885, 903
MunI CAATTG 2 cut(s) 330, 615
MvaI CCWGG 3 cut(s) 122, 206, 885
MvnI CGCG 3 cut(s) 942, 944, 1449
MwoI GCNNNNNNNGC 2 cut(s) 1208, 1238
NciI CCSGG 1 cut(s) 903
NdeII GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
NlaIII CATG 9 cut(s) 25, 298, 457, 599, 646, 809, 832, 1237, 1374
NlaIV GGNNCC 2 cut(s) 111, 970
NmeAIII GCCGAG 1 cut(s) 1108
NspI RCATGY 2 cut(s) 298, 809
PaeR7I CTCGAG 2 cut(s) 38, 870
PagI TCATGA 1 cut(s) 453
PciSI GCTCTTC 1 cut(s) 855
PcsI WCGNNNNNNNCGW 1 cut(s) 946
PdmI GAANNNNTTC 3 cut(s) 879, 967, 1349
PfeI GAWTC 1 cut(s) 481
PflFI GACNNNGTC 1 cut(s) 165
PfoI TCCNGGA 1 cut(s) 883
PkrI GCNGC 6 cut(s) 305, 499, 526, 628, 868, 1448
PleI GAGTC 2 cut(s) 1156, 1445
PmaCI CACGTG 1 cut(s) 1397
PmlI CACGTG 1 cut(s) 1397
PpsI GAGTC 2 cut(s) 1156, 1445
Ppu21I YACGTR 1 cut(s) 1397
Psp6I CCWGG 3 cut(s) 120, 204, 883
PspCI CACGTG 1 cut(s) 1397
PspEI GGTNACC 1 cut(s) 700
PspGI CCWGG 3 cut(s) 120, 204, 883
PspN4I GGNNCC 2 cut(s) 111, 970
PspPI GGNCC 3 cut(s) 80, 110, 341
PsuI RGATCY 3 cut(s) 919, 1030, 1273
PsyI GACNNNGTC 1 cut(s) 165
RsaI GTAC 2 cut(s) 119, 270
RsaNI GTAC 2 cut(s) 118, 269
RseI CAYNNNNRTG 2 cut(s) 1109, 1179
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 4 cut(s) 132, 312, 581, 1107
SatI GCNGC 6 cut(s) 304, 498, 525, 627, 867, 1447
Sau3AI GATC 9 cut(s) 42, 152, 592, 686, 727, 919, 1030, 1273, 1432
Sau96I GGNCC 3 cut(s) 80, 110, 341
SchI GAGTC 2 cut(s) 1156, 1445
ScrFI CCNGG 4 cut(s) 122, 206, 885, 903
SfaNI GCATC 2 cut(s) 835, 1311
SfcI CTRYAG 1 cut(s) 1441
Sfr274I CTCGAG 2 cut(s) 38, 870
SinI GGWCC 2 cut(s) 80, 110
SlaI CTCGAG 2 cut(s) 38, 870
SmiMI CAYNNNNRTG 2 cut(s) 1109, 1179
SmlI CTYRAG 2 cut(s) 38, 870
SmoI CTYRAG 2 cut(s) 38, 870
SpeI ACTAGT 1 cut(s) 11
SsiI CCGC 5 cut(s) 304, 559, 774, 944, 1447
SspI AATATT 2 cut(s) 136, 782
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 4 cut(s) 120, 204, 883, 901
TaaI ACNGT 3 cut(s) 64, 250, 1412
TaiI ACGT 5 cut(s) 166, 895, 952, 1113, 1399
TaqI TCGA 5 cut(s) 18, 39, 689, 757, 871
TauI GCSGC 2 cut(s) 306, 1449
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 4 cut(s) 132, 312, 581, 1107
Tru9I TTAA 4 cut(s) 132, 312, 581, 1107
TscAI CASTG 2 cut(s) 403, 1383
TseI GCWGC 4 cut(s) 497, 524, 626, 866
TspDTI ATGAA 5 cut(s) 470, 807, 951, 1151, 1250
TspGWI ACGGA 1 cut(s) 1373
TspRI CASTG 2 cut(s) 403, 1383
Tth111I GACNNNGTC 1 cut(s) 165
VpaK11BI GGWCC 2 cut(s) 80, 110
XapI RAATTY 2 cut(s) 531, 1301
XceI RCATGY 2 cut(s) 298, 809
XhoI CTCGAG 2 cut(s) 38, 870
XmiI GTMKAC 2 cut(s) 505, 914
XmnI GAANNNNTTC 3 cut(s) 879, 967, 1349
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.