MD05G1124700.v1.1

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007224
Physical Location & Seq
Forward (+)
24683299 .. 24688543
5245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1124700.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGACGAGGGAAACCTAGAGGTCGGAATGGAATACAGAACTGTGTCTGGTGTGGCCGGACCTCTGGTTATCCTTGAAAAAGTTAAGGGACCTAAGTTTCAAGAGATTGTTAATATTCGTTTGGGAGATGGAACAACTCGACGTGGTCAGGTCCTGGAAGTTGATGGAGAGAAAGCTATTGTACAGGTTTTCGAAGGAACATCTGGAATTGACAACAAGTACACTACTGTGCAATTCACAGGAGAGGTTTTGAAAACTCCAGTCTCACTTGACATGCTTGGGCGCATCTTTAATGGCTCTGGGAAGCCCATTGATAATGGCCCCCCTATTTTGCCTGAGGCTTACCTAGACATATCTGGGAGTTCTATTAATCCTAGTGAGAGAACATATCCTGAAGAAATGATTCAGACTGGAATTTCTACTATTGATGTCATGAACTCCATTGCTAGAGGACAAAAAATCCCCCTTTTCTCTGCTGCTGGTCTTCCTCATAATGAAATAGCTGCTCAGATATGTCGCCAGGCCGGTTTGGTCAAGCGGTTGGAGAAATCTGAGAGTCTTCTTGACGCTGGGGACGTAGAAGACGACAACTTTGCCATTGTGTTTGCAGCTATGGGAGTAAATATGGAGACTGCACAGTTCTTTAAGCGTGATTTTGAGGAAAATGGTTCAATGGAGAGAGTGACCCTTTTTCTGAATCTGGCAAATGACCCTACAATTGAACGCATTATTACTCCTCGTATTGCTCTTACTACTGCAGAATATTTGGCATATGAATGTGGGAAGCACGTTCTTGTCATTCTCACAGATATGAGTTCTTATGCTGACGCTCTTCGTGAGGTGTCTGCTGCCCGAGAGGAAGTGCCGGGAAGGCGTGGATACCCTGGGTACATGTATACTGATCTGGCACAAATCTATGAGCGTGCTGGACGAATTGAAGGGCGAAAAGGCTCTATTACCCAAATTCCAATTTTAACTATGCCAAATGATGATATTACCCACCCCACTCCAGATCTTACTGGATATATTACTGAGGGACAGATATACATTGACAGGCAGCTCCACAACAGACAGATATACCCACCAATCAATGTCCTTCCATCACTATCTCGTCTGATGAAGAGTGCTATTGGTGAAGGCATGACTCGCCGGGATCATTCTGATGTATCAAATCAGTTATATGCAAATTATGCTATTGGGAAGGATGTCCAGGCAATGAAAGCTGTGGTCGGAGAAGAAGCACTTTCCTCGGAGGACTTGCTATACCTGGAGTTCTTGGACAAATTTGAGAAGAAGTTTGTGTCCCAAGGAGCCTATGACACCCGTAACATCTTCCAGTCCCTTGATTTGGCATGGACGTTGCTGCGAATCTTCCCCCGTGAGCTTCTCCACCGTATACCTGCGAAGACCCTTGACCTGTTCTACAGCAGAGATGCAACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001678 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0008064 GO:0008092 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010182 GO:0010255 GO:0010468 GO:0010556 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0019219 GO:0019222 GO:0019725 GO:0022607 GO:0023052 GO:0030029 GO:0030036 GO:0030054 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031090 GO:0031323 GO:0031326 GO:0031333 GO:0032271 GO:0032272 GO:0032535 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033500 GO:0034284 GO:0042221 GO:0042592 GO:0042593 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043242 GO:0043244 GO:0043254 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046686 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051493 GO:0051494 GO:0051693 GO:0051716 GO:0055044 GO:0055082 GO:0060255 GO:0061572 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0097435 GO:0098588 GO:0098805 GO:0110053 GO:1901700 GO:1901701 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N

Protein Analysis

482

Amino Acids

53.51

Weight (kDa)

4.98

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 15 - 81 4.1e-15 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 138 - 370 1.1e-75 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 376 - 474 7e-34 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1408
AccI GTMKAC 2 cut(s) 896, 1396
AciI CCGC 1 cut(s) 538
AclWI GGATC 1 cut(s) 1161
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 3 cut(s) 414, 963, 1283
AcuI CTGAAG 1 cut(s) 414
AfaI GTAC 3 cut(s) 183, 221, 890
AfiI CCNNNNNNNGG 1 cut(s) 1348
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 6 cut(s) 77, 101, 253, 672, 722, 938
AjiI CACGTC 1 cut(s) 143
AjnI CCWGG 5 cut(s) 153, 519, 883, 1209, 1266
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 6 cut(s) 176, 503, 611, 1060, 1223, 1384
AluI AGCT 6 cut(s) 176, 503, 611, 1060, 1223, 1384
Alw26I GTCTC 2 cut(s) 268, 623
AlwI GGATC 1 cut(s) 1161
AlwNI CAGNNNCTG 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 852
AoxI GGCC 3 cut(s) 54, 319, 522
ApeKI GCWGC 6 cut(s) 476, 503, 608, 848, 1057, 1363
ApoI RAATTY 3 cut(s) 414, 963, 1283
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AseI ATTAAT 1 cut(s) 369
Asp700I GAANNNNTTC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 4 cut(s) 59, 89, 151, 320
AsuC2I CCSGG 2 cut(s) 867, 1151
AsuHPI GGTGA 1 cut(s) 1145
AsuII TTCGAA 1 cut(s) 192
AvaI CYCGRG 1 cut(s) 852
AvaII GGWCC 3 cut(s) 59, 89, 151
AxyI CCTNAGG 1 cut(s) 336
BbsI GAAGAC 4 cut(s) 476, 551, 588, 1412
BbvI GCAGC 6 cut(s) 463, 490, 620, 835, 1069, 1350
BccI CCATC 3 cut(s) 122, 158, 1108
BcgI CGANNNNNNTGC 4 cut(s) 575, 609, 1230, 1264
BciT130I CCWGG 5 cut(s) 155, 521, 885, 1211, 1268
BciVI GTATCC 1 cut(s) 872
BcnI CCSGG 2 cut(s) 867, 1151
BcoDI GTCTC 2 cut(s) 268, 623
BfaI CTAG 4 cut(s) 17, 347, 375, 447
BfmI CTRYAG 2 cut(s) 756, 1423
BfuAI ACCTGC 1 cut(s) 1408
BfuI GTATCC 1 cut(s) 872
BglI GCCNNNNNGGC 1 cut(s) 871
BglII AGATCT 1 cut(s) 1012
BisI GCNGC 6 cut(s) 477, 504, 609, 849, 1058, 1364
BlsI GCNGC 6 cut(s) 478, 505, 610, 850, 1059, 1365
Bme1390I CCNGG 7 cut(s) 155, 521, 867, 885, 1151, 1211, 1268
Bme18I GGWCC 3 cut(s) 59, 89, 151
BmeT110I CYCGRG 1 cut(s) 852
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 4 cut(s) 59, 89, 151, 320
BmiI GGNNCC 3 cut(s) 90, 322, 1312
BmrFI CCNGG 7 cut(s) 155, 521, 867, 885, 1151, 1211, 1268
BmsI GCATC 2 cut(s) 294, 1423
BpiI GAAGAC 4 cut(s) 476, 551, 588, 1412
BpmI CTGGAG 3 cut(s) 243, 993, 1289
Bpu14I TTCGAA 1 cut(s) 192
BpuMI CCSGG 2 cut(s) 867, 1151
BsaJI CCNNGG 4 cut(s) 883, 884, 1248, 1306
Bsc4I CCNNNNNNNGG 1 cut(s) 1348
Bse118I RCCGGY 1 cut(s) 524
Bse1I ACTGG 4 cut(s) 260, 415, 1024, 1336
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 2 cut(s) 441, 1221
BseBI CCWGG 5 cut(s) 155, 521, 885, 1211, 1268
BseDI CCNNGG 4 cut(s) 883, 884, 1248, 1306
BseGI GGATG 1 cut(s) 1210
BseLI CCNNNNNNNGG 1 cut(s) 1348
BseMI GCAATG 2 cut(s) 441, 1221
BseMII CTCAG 4 cut(s) 327, 521, 543, 1023
BseNI ACTGG 4 cut(s) 260, 415, 1024, 1336
BseRI GAGGAG 1 cut(s) 726
BseXI GCAGC 6 cut(s) 463, 490, 620, 835, 1069, 1350
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 618
BshFI GGCC 3 cut(s) 56, 321, 524
BsiHKCI CYCGRG 1 cut(s) 852
BsiSI CCGG 4 cut(s) 57, 525, 866, 1150
BslFI GGGAC 5 cut(s) 102, 587, 1050, 1288, 1324
BslI CCNNNNNNNGG 1 cut(s) 1348
BsmAI GTCTC 2 cut(s) 268, 623
BsmFI GGGAC 5 cut(s) 102, 587, 1050, 1288, 1324
BsnI GGCC 3 cut(s) 56, 321, 524
BsoBI CYCGRG 1 cut(s) 852
Bsp119I TTCGAA 1 cut(s) 192
Bsp1407I TGTACA 1 cut(s) 181
Bsp143I GATC 3 cut(s) 901, 1012, 1153
BspACI CCGC 1 cut(s) 538
BspANI GGCC 3 cut(s) 56, 321, 524
BspCNI CTCAG 4 cut(s) 328, 520, 544, 1024
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 3 cut(s) 90, 322, 1312
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 1408
BspPI GGATC 1 cut(s) 1161
BspQI GCTCTTC 1 cut(s) 837
BspT104I TTCGAA 1 cut(s) 192
BsrDI GCAATG 2 cut(s) 441, 1221
BsrFI RCCGGY 1 cut(s) 524
BsrGI TGTACA 1 cut(s) 181
BsrI ACTGG 4 cut(s) 260, 415, 1024, 1336
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 4 cut(s) 883, 884, 1248, 1306
BssMI GATC 3 cut(s) 901, 1012, 1153
BssNAI GTATAC 2 cut(s) 897, 1397
BssT1I CCWWGG 1 cut(s) 1306
Bst1107I GTATAC 2 cut(s) 897, 1397
Bst2UI CCWGG 5 cut(s) 155, 521, 885, 1211, 1268
Bst4CI ACNGT 4 cut(s) 43, 229, 639, 1394
Bst6I CTCTTC 2 cut(s) 837, 1115
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstAUI TGTACA 1 cut(s) 181
BstBI TTCGAA 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 924
BstDEI CTNAG 5 cut(s) 93, 336, 507, 552, 1032
BstF5I GGATG 1 cut(s) 1210
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 3 cut(s) 904, 1015, 1156
BstMAI GTCTC 2 cut(s) 268, 623
BstMBI GATC 3 cut(s) 901, 1012, 1153
BstMWI GCNNNNNNNGC 4 cut(s) 871, 1146, 1190, 1220
BstNI CCWGG 5 cut(s) 155, 521, 885, 1211, 1268
BstNSI RCATGY 2 cut(s) 277, 895
BstSCI CCNGG 7 cut(s) 153, 519, 865, 883, 1149, 1209, 1266
BstSFI CTRYAG 2 cut(s) 756, 1423
BstV1I GCAGC 6 cut(s) 463, 490, 620, 835, 1069, 1350
BstV2I GAAGAC 4 cut(s) 476, 551, 588, 1412
BstX2I RGATCY 1 cut(s) 1012
BstYI RGATCY 1 cut(s) 1012
BstZ17I GTATAC 2 cut(s) 897, 1397
Bsu36I CCTNAGG 1 cut(s) 336
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 3 cut(s) 56, 321, 524
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 1 cut(s) 1210
BveI ACCTGC 1 cut(s) 1408
Cac8I GCNNGC 1 cut(s) 924
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 1 cut(s) 432
CfoI GCGC 1 cut(s) 285
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 4 cut(s) 59, 89, 151, 320
CseI GACGC 2 cut(s) 575, 836
Csp6I GTAC 3 cut(s) 182, 220, 889
CviAII CATG 5 cut(s) 274, 433, 892, 1141, 1353
CviQI GTAC 3 cut(s) 182, 220, 889
DdeI CTNAG 5 cut(s) 93, 336, 507, 552, 1032
DpnI GATC 3 cut(s) 903, 1014, 1155
DpnII GATC 3 cut(s) 901, 1012, 1153
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 837, 1115
EarI CTCTTC 2 cut(s) 837, 1115
Eco130I CCWWGG 1 cut(s) 1306
Eco47I GGWCC 3 cut(s) 59, 89, 151
Eco57I CTGAAG 1 cut(s) 414
Eco81I CCTNAGG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 852
EcoO109I RGGNCCY 2 cut(s) 89, 151
EcoRII CCWGG 5 cut(s) 153, 519, 883, 1209, 1266
EcoT14I CCWWGG 1 cut(s) 1306
ErhI CCWWGG 1 cut(s) 1306
FaeI CATG 5 cut(s) 277, 436, 895, 1144, 1356
FalI AAGNNNNNCTT 2 cut(s) 1227, 1259
FaqI GGGAC 5 cut(s) 102, 587, 1050, 1288, 1324
FatI CATG 5 cut(s) 273, 432, 891, 1140, 1352
FauNDI CATATG 1 cut(s) 772
FblI GTMKAC 2 cut(s) 896, 1396
Fnu4HI GCNGC 6 cut(s) 477, 504, 609, 849, 1058, 1364
FokI GGATG 1 cut(s) 1217
Fsp4HI GCNGC 6 cut(s) 477, 504, 609, 849, 1058, 1364
FspBI CTAG 4 cut(s) 17, 347, 375, 447
GlaI GCGC 1 cut(s) 284
GluI GCNGC 6 cut(s) 477, 504, 609, 849, 1058, 1364
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 3 cut(s) 243, 993, 1289
HaeIII GGCC 3 cut(s) 56, 321, 524
HapII CCGG 4 cut(s) 57, 525, 866, 1150
HgaI GACGC 2 cut(s) 575, 836
HhaI GCGC 1 cut(s) 285
Hin1II CATG 5 cut(s) 277, 436, 895, 1144, 1356
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HinfI GANTC 5 cut(s) 403, 556, 697, 1144, 1368
HpaII CCGG 4 cut(s) 57, 525, 866, 1150
HphI GGTGA 1 cut(s) 1145
Hpy166II GTNNAC 3 cut(s) 222, 897, 1397
Hpy188I TCNGA 9 cut(s) 26, 408, 510, 553, 696, 1116, 1162, 1232, 1252
Hpy188III TCNNGA 7 cut(s) 101, 204, 392, 433, 563, 836, 1010
Hpy8I GTNNAC 3 cut(s) 222, 897, 1397
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 6 cut(s) 188, 864, 932, 1106, 1130, 1195
HpyCH4III ACNGT 4 cut(s) 43, 229, 639, 1394
HpyCH4IV ACGT 4 cut(s) 142, 576, 789, 1358
HpyCH4V TGCA 6 cut(s) 232, 608, 635, 758, 1184, 1436
HpyF10VI GCNNNNNNNGC 4 cut(s) 871, 1146, 1190, 1220
HpyF3I CTNAG 5 cut(s) 93, 336, 507, 552, 1032
HpySE526I ACGT 4 cut(s) 142, 576, 789, 1358
Hsp92II CATG 5 cut(s) 277, 436, 895, 1144, 1356
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 3 cut(s) 901, 1012, 1153
LguI GCTCTTC 1 cut(s) 837
LmnI GCTCC 2 cut(s) 1065, 1310
Lsp1109I GCAGC 6 cut(s) 463, 490, 620, 835, 1069, 1350
LweI GCATC 2 cut(s) 294, 1423
MaeI CTAG 4 cut(s) 17, 347, 375, 447
MaeII ACGT 4 cut(s) 142, 576, 789, 1358
MaeIII GTNAC 2 cut(s) 682, 1325
MalI GATC 3 cut(s) 903, 1014, 1155
MboI GATC 3 cut(s) 901, 1012, 1153
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 1012
MlyI GAGTC 2 cut(s) 565, 1138
MmeI TCCRAC 3 cut(s) 4, 522, 1210
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 6 cut(s) 84, 111, 291, 369, 645, 974
MslI CAYNNNNRTG 4 cut(s) 227, 775, 809, 1161
MspI CCGG 4 cut(s) 57, 525, 866, 1150
MspR9I CCNGG 7 cut(s) 155, 521, 867, 885, 1151, 1211, 1268
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 5 cut(s) 155, 521, 885, 1211, 1268
MwoI GCNNNNNNNGC 4 cut(s) 871, 1146, 1190, 1220
NciI CCSGG 2 cut(s) 867, 1151
NdeI CATATG 1 cut(s) 772
NdeII GATC 3 cut(s) 901, 1012, 1153
NlaIII CATG 5 cut(s) 277, 436, 895, 1144, 1356
NlaIV GGNNCC 3 cut(s) 90, 322, 1312
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 2 cut(s) 277, 895
NspV TTCGAA 1 cut(s) 192
OliI CACNNNNGTG 1 cut(s) 227
PagI TCATGA 1 cut(s) 432
PasI CCCWGGG 1 cut(s) 884
PciI ACATGT 1 cut(s) 891
PciSI GCTCTTC 1 cut(s) 837
PcsI WCGNNNNNNNCGW 2 cut(s) 573, 582
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 3 cut(s) 403, 697, 1368
PflFI GACNNNGTC 1 cut(s) 144
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 6 cut(s) 478, 505, 610, 850, 1059, 1365
PleI GAGTC 2 cut(s) 564, 1138
PpsI GAGTC 2 cut(s) 564, 1138
PpuMI RGGWCCY 2 cut(s) 89, 151
PscI ACATGT 1 cut(s) 891
PshBI ATTAAT 1 cut(s) 369
Psp5II RGGWCCY 2 cut(s) 89, 151
Psp6I CCWGG 5 cut(s) 153, 519, 883, 1209, 1266
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 5 cut(s) 153, 519, 883, 1209, 1266
PspN4I GGNNCC 3 cut(s) 90, 322, 1312
PspPI GGNCC 4 cut(s) 59, 89, 151, 320
PspPPI RGGWCCY 2 cut(s) 89, 151
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 1012
PsyI GACNNNGTC 1 cut(s) 144
RsaI GTAC 3 cut(s) 183, 221, 890
RsaNI GTAC 3 cut(s) 182, 220, 889
RseI CAYNNNNRTG 4 cut(s) 227, 775, 809, 1161
SapI GCTCTTC 1 cut(s) 837
SaqAI TTAA 6 cut(s) 84, 111, 291, 369, 645, 974
SatI GCNGC 6 cut(s) 477, 504, 609, 849, 1058, 1364
Sau3AI GATC 3 cut(s) 901, 1012, 1153
Sau96I GGNCC 4 cut(s) 59, 89, 151, 320
SchI GAGTC 2 cut(s) 565, 1138
ScrFI CCNGG 7 cut(s) 155, 521, 867, 885, 1151, 1211, 1268
SfaNI GCATC 2 cut(s) 294, 1423
SfcI CTRYAG 2 cut(s) 756, 1423
SfuI TTCGAA 1 cut(s) 192
SinI GGWCC 3 cut(s) 59, 89, 151
SmiMI CAYNNNNRTG 4 cut(s) 227, 775, 809, 1161
SsiI CCGC 1 cut(s) 538
SspI AATATT 2 cut(s) 115, 764
SspMI CTAG 4 cut(s) 17, 347, 375, 447
StyD4I CCNGG 7 cut(s) 153, 519, 865, 883, 1149, 1209, 1266
StyI CCWWGG 1 cut(s) 1306
TaaI ACNGT 4 cut(s) 43, 229, 639, 1394
TaiI ACGT 4 cut(s) 145, 579, 792, 1361
TaqI TCGA 2 cut(s) 139, 192
TatI WGTACW 2 cut(s) 181, 219
TfiI GAWTC 3 cut(s) 403, 697, 1368
Tru1I TTAA 6 cut(s) 84, 111, 291, 369, 645, 974
Tru9I TTAA 6 cut(s) 84, 111, 291, 369, 645, 974
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 6 cut(s) 476, 503, 608, 848, 1057, 1363
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 5 cut(s) 449, 510, 789, 1133, 1232
Tth111I GACNNNGTC 1 cut(s) 144
VpaK11BI GGWCC 3 cut(s) 59, 89, 151
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 3 cut(s) 414, 963, 1283
XceI RCATGY 2 cut(s) 277, 895
XmiI GTMKAC 2 cut(s) 896, 1396
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 4 cut(s) 17, 347, 375, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.