Prupe.8G168500_v2.0.a1

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0007224
Physical Location & Seq
Forward (+)
17381607 .. 17386579
4973 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G168500.2

Sequence Viewer

Length: 1473 bp
ATGGCTGTTTCACAAAACAATCACGACATGGACGAGGGACAACTAGAGGTCGGCATGGAGTACAGAACTGTTTCTGGTGTGGCCGGACCTCTGGTTATCCTTGAAAAAGTTAAGGGACCTAAGTTTCAGGAGATTGTTAATATTCGTTTGGGAGATGGAACAACTCGACGTGGTCAAGTCCTGGAGGTTGATGGAGAGAAAGCTATTGTTCAGGTTTTTGAAGGAACATCTGGAATTGACAACAAGTACACCACTGTGCAATTTACAGGAGAGGTTTTGAAAACTCCAGTCTCACTGGACATGCTTGGGCGCATCTTTAATGGTTCTGGGAAACCCATTGATAATGGCCCCCCTATTTTGCCTGAGGCTTACCTAGACATATCTGGGAGTTCTATCAATCCTAGTGAGAGAACATATCCTGAAGAGATGATACAGACTGGAATTTCTACAATTGATGTCATGAATTCCATTGCCAGAGGACAAAAAATCCCACTTTTCTCTGCTGCTGGTCTTCCTCATAACGAAATAGCTGCTCAGATATGTCGCCAGGCTGGTTTGGTCAAGCGGTTGGAGAAATCTGACAGTCTTCTTGACGCTGTGGATGTGGAAGACGACAACTTTGCCATTGTGTTTGCAGCTATGGGTGTAAATATGGAGACAGCACAGTTTTTCAAACGTGATTTTGAAGAAAATGGTTCAATGGAGAGAGTGACCCTTTTTCTGAATCTGGCAAATGACCCTACAATTGAACGTATTATTACTCCTCGTATTGCTCTTACTACTGCAGAATATTTGGCATATGAATGTGGGAAGCATGTTCTTGTCATTCTCACAGATATGAGTTCTTATGCTGATGCTCTTCGTGAGGTATCTGCTGCTCGAGAAGAAGTGCCTGGAAGGCGTGGATATCCAGGGTATATGTATACTGATCTGGCCCAAATCTATGAGCGTGCCGGACGAATTGAAGGCAGAAAAGGCTCCATTACCCAAATTCCGATTTTAACTATGCCAAACGATGATATTACACACCCCACTCCAGATCTTACAGGATATATTACTGAGGGACAGATATACATTGACAGGCAGCTCTACAACAGACAGATATACCCGCCAATCAATGTTCTCCCATCACTGTCTCGTCTGATGAAGAGTGCTATTGGTGAAGGGATGACTCGCAGGGATCATTCTGATGTATCCAACCAGTTATATGCAAATTATGCTATTGGGAAGGATGTCCAGGCAATGAAGGCTGTGGTTGGAGAAGAAGCACTTTCTTCGGAGGACCTGCTATACCTGGAGTTCTTGGACAAATTTGAGAAGAAGTTTGTGAGCCAAGGAGCCTATGACACCCGTAATATCTTCCAGTCACTTGATTTGGCATGGACGTTGCTGCGGATCTTCCCTCGTGAGCTTCTCCACCGTATACCTGCAAAGACTCTTGATCTGTTCTACAGCCGAGATGCAGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001678 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0008064 GO:0008092 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010182 GO:0010255 GO:0010468 GO:0010556 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0019219 GO:0019222 GO:0019725 GO:0022607 GO:0023052 GO:0030029 GO:0030036 GO:0030054 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031090 GO:0031323 GO:0031326 GO:0031333 GO:0032271 GO:0032272 GO:0032535 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033500 GO:0034284 GO:0042221 GO:0042592 GO:0042593 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043242 GO:0043244 GO:0043254 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046686 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051493 GO:0051494 GO:0051693 GO:0051716 GO:0055044 GO:0055082 GO:0060255 GO:0061572 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0097435 GO:0098588 GO:0098805 GO:0110053 GO:1901700 GO:1901701 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N

Protein Analysis

491

Amino Acids

54.54

Weight (kDa)

4.94

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1293, 1435
AccI GTMKAC 2 cut(s) 923, 1423
AciI CCGC 3 cut(s) 565, 1109, 1393
AclWI GGATC 2 cut(s) 1188, 1403
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 4 cut(s) 441, 463, 990, 1310
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 62, 248
AgsI TTSAA 8 cut(s) 104, 221, 280, 673, 686, 699, 749, 965
AjiI CACGTC 1 cut(s) 170
AjnI CCWGG 6 cut(s) 180, 546, 892, 910, 1236, 1293
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 6 cut(s) 203, 530, 638, 1087, 1411, 1466
AluI AGCT 6 cut(s) 203, 530, 638, 1087, 1411, 1466
Alw26I GTCTC 3 cut(s) 295, 650, 1140
AlwI GGATC 2 cut(s) 1188, 1403
Ama87I CYCGRG 1 cut(s) 879
AoxI GGCC 3 cut(s) 81, 346, 933
ApeKI GCWGC 7 cut(s) 503, 530, 635, 875, 1084, 1390, 1463
ApoI RAATTY 4 cut(s) 441, 463, 990, 1310
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
Asp700I GAANNNNTTC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 5 cut(s) 86, 116, 347, 934, 1282
AsuHPI GGTGA 1 cut(s) 1172
AvaI CYCGRG 1 cut(s) 879
AvaII GGWCC 3 cut(s) 86, 116, 1282
AxyI CCTNAGG 1 cut(s) 363
BarI GAAGNNNNNNTAC 2 cut(s) 414, 446
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 3 cut(s) 503, 578, 615
BbvI GCAGC 6 cut(s) 490, 517, 647, 862, 1096, 1377
BccI CCATC 3 cut(s) 149, 185, 1135
BcgI CGANNNNNNTGC 6 cut(s) 512, 546, 602, 636, 1257, 1291
BciT130I CCWGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
BciVI GTATCC 1 cut(s) 1204
BcoDI GTCTC 3 cut(s) 295, 650, 1140
BfaI CTAG 3 cut(s) 44, 374, 402
BfmI CTRYAG 2 cut(s) 783, 1450
BfuAI ACCTGC 2 cut(s) 1293, 1435
BfuI GTATCC 1 cut(s) 1204
BglI GCCNNNNNGGC 1 cut(s) 898
BglII AGATCT 1 cut(s) 1039
BisI GCNGC 7 cut(s) 504, 531, 636, 876, 1085, 1391, 1464
BlsI GCNGC 7 cut(s) 505, 532, 637, 877, 1086, 1392, 1465
Bme1390I CCNGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
Bme18I GGWCC 3 cut(s) 86, 116, 1282
BmeT110I CYCGRG 1 cut(s) 879
BmgBI CACGTC 1 cut(s) 170
BmgT120I GGNCC 5 cut(s) 86, 116, 347, 934, 1282
BmiI GGNNCC 4 cut(s) 117, 349, 979, 1339
BmrFI CCNGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
BmsI GCATC 3 cut(s) 321, 844, 1450
BpiI GAAGAC 3 cut(s) 503, 578, 615
BpmI CTGGAG 4 cut(s) 203, 270, 1020, 1316
BsaJI CCNNGG 2 cut(s) 911, 1333
Bse1I ACTGG 5 cut(s) 287, 300, 442, 1201, 1363
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 2 cut(s) 468, 1248
BseBI CCWGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
BseDI CCNNGG 2 cut(s) 911, 1333
BseGI GGATG 3 cut(s) 607, 1173, 1237
BseMI GCAATG 2 cut(s) 468, 1248
BseMII CTCAG 3 cut(s) 354, 548, 1050
BseNI ACTGG 5 cut(s) 287, 300, 442, 1201, 1363
BseRI GAGGAG 1 cut(s) 753
BseXI GCAGC 6 cut(s) 490, 517, 647, 862, 1096, 1377
BshFI GGCC 3 cut(s) 83, 348, 935
BsiHKCI CYCGRG 1 cut(s) 879
BsiSI CCGG 2 cut(s) 84, 954
BslFI GGGAC 3 cut(s) 51, 129, 1077
BsmAI GTCTC 3 cut(s) 295, 650, 1140
BsmFI GGGAC 3 cut(s) 51, 129, 1077
BsnI GGCC 3 cut(s) 83, 348, 935
BsoBI CYCGRG 1 cut(s) 879
Bsp143I GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
BspACI CCGC 3 cut(s) 565, 1109, 1393
BspANI GGCC 3 cut(s) 83, 348, 935
BspCNI CTCAG 3 cut(s) 355, 547, 1051
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 4 cut(s) 117, 349, 979, 1339
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 2 cut(s) 1293, 1435
BspPI GGATC 2 cut(s) 1188, 1403
BspQI GCTCTTC 1 cut(s) 864
BsrDI GCAATG 2 cut(s) 468, 1248
BsrI ACTGG 5 cut(s) 287, 300, 442, 1201, 1363
BssECI CCNNGG 2 cut(s) 911, 1333
BssMI GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
BssNAI GTATAC 2 cut(s) 924, 1424
BssSI CACGAG 1 cut(s) 1404
BssT1I CCWWGG 1 cut(s) 1333
Bst1107I GTATAC 2 cut(s) 924, 1424
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
Bst4CI ACNGT 6 cut(s) 70, 256, 584, 666, 1134, 1421
Bst6I CTCTTC 3 cut(s) 417, 864, 1142
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstC8I GCNNGC 1 cut(s) 951
BstDEI CTNAG 4 cut(s) 120, 363, 534, 1059
BstF5I GGATG 3 cut(s) 607, 1173, 1237
BstHHI GCGC 1 cut(s) 312
BstKTI GATC 5 cut(s) 931, 1042, 1183, 1398, 1444
BstMAI GTCTC 3 cut(s) 295, 650, 1140
BstMBI GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
BstMWI GCNNNNNNNGC 4 cut(s) 898, 975, 1217, 1247
BstNI CCWGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
BstNSI RCATGY 2 cut(s) 304, 818
BstSCI CCNGG 6 cut(s) 180, 546, 892, 910, 1236, 1293
BstSFI CTRYAG 2 cut(s) 783, 1450
BstV1I GCAGC 6 cut(s) 490, 517, 647, 862, 1096, 1377
BstV2I GAAGAC 3 cut(s) 503, 578, 615
BstX2I RGATCY 2 cut(s) 1039, 1395
BstYI RGATCY 2 cut(s) 1039, 1395
BstZ17I GTATAC 2 cut(s) 924, 1424
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1204
BsuRI GGCC 3 cut(s) 83, 348, 935
BtrI CACGTC 1 cut(s) 170
BtsCI GGATG 3 cut(s) 607, 1173, 1237
BtsIMutI CAGTG 3 cut(s) 252, 293, 1130
BveI ACCTGC 2 cut(s) 1293, 1435
Cac8I GCNNGC 1 cut(s) 951
CciI TCATGA 1 cut(s) 459
CfoI GCGC 1 cut(s) 312
Cfr13I GGNCC 5 cut(s) 86, 116, 347, 934, 1282
CseI GACGC 1 cut(s) 602
Csp6I GTAC 2 cut(s) 61, 247
CviAII CATG 6 cut(s) 28, 55, 301, 460, 815, 1380
CviQI GTAC 2 cut(s) 61, 247
DdeI CTNAG 4 cut(s) 120, 363, 534, 1059
DpnI GATC 5 cut(s) 930, 1041, 1182, 1397, 1443
DpnII GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 417, 864, 1142
EarI CTCTTC 3 cut(s) 417, 864, 1142
Eco130I CCWWGG 1 cut(s) 1333
Eco32I GATATC 1 cut(s) 908
Eco47I GGWCC 3 cut(s) 86, 116, 1282
Eco57I CTGAAG 1 cut(s) 441
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 879
EcoO109I RGGNCCY 2 cut(s) 116, 1282
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 6 cut(s) 180, 546, 892, 910, 1236, 1293
EcoRV GATATC 1 cut(s) 908
EcoT14I CCWWGG 1 cut(s) 1333
ErhI CCWWGG 1 cut(s) 1333
FaeI CATG 6 cut(s) 31, 58, 304, 463, 818, 1383
FalI AAGNNNNNCTT 2 cut(s) 1254, 1286
FaqI GGGAC 3 cut(s) 51, 129, 1077
FatI CATG 6 cut(s) 27, 54, 300, 459, 814, 1379
FauI CCCGC 1 cut(s) 1116
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 2 cut(s) 923, 1423
Fnu4HI GCNGC 7 cut(s) 504, 531, 636, 876, 1085, 1391, 1464
FokI GGATG 3 cut(s) 614, 1180, 1244
Fsp4HI GCNGC 7 cut(s) 504, 531, 636, 876, 1085, 1391, 1464
FspBI CTAG 3 cut(s) 44, 374, 402
GlaI GCGC 1 cut(s) 311
GluI GCNGC 7 cut(s) 504, 531, 636, 876, 1085, 1391, 1464
GsuI CTGGAG 4 cut(s) 203, 270, 1020, 1316
HaeIII GGCC 3 cut(s) 83, 348, 935
HapII CCGG 2 cut(s) 84, 954
HgaI GACGC 1 cut(s) 602
HhaI GCGC 1 cut(s) 312
Hin1II CATG 6 cut(s) 31, 58, 304, 463, 818, 1383
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HinfI GANTC 3 cut(s) 724, 1171, 1435
HpaII CCGG 2 cut(s) 84, 954
HphI GGTGA 1 cut(s) 1172
Hpy166II GTNNAC 3 cut(s) 249, 924, 1424
Hpy188I TCNGA 7 cut(s) 537, 580, 723, 996, 1143, 1189, 1279
Hpy8I GTNNAC 3 cut(s) 249, 924, 1424
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 6 cut(s) 215, 891, 959, 1157, 1222, 1240
HpyCH4III ACNGT 6 cut(s) 70, 256, 584, 666, 1134, 1421
HpyCH4IV ACGT 4 cut(s) 169, 676, 751, 1385
HpyCH4V TGCA 6 cut(s) 259, 635, 785, 1211, 1430, 1463
HpyF10VI GCNNNNNNNGC 4 cut(s) 898, 975, 1217, 1247
HpyF3I CTNAG 4 cut(s) 120, 363, 534, 1059
HpySE526I ACGT 4 cut(s) 169, 676, 751, 1385
Hsp92II CATG 6 cut(s) 31, 58, 304, 463, 818, 1383
HspAI GCGC 1 cut(s) 310
Kzo9I GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 2 cut(s) 983, 1337
Lsp1109I GCAGC 6 cut(s) 490, 517, 647, 862, 1096, 1377
LweI GCATC 3 cut(s) 321, 844, 1450
MaeI CTAG 3 cut(s) 44, 374, 402
MaeII ACGT 4 cut(s) 169, 676, 751, 1385
MaeIII GTNAC 2 cut(s) 709, 1365
MalI GATC 5 cut(s) 930, 1041, 1182, 1397, 1443
MboI GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
MfeI CAATTG 2 cut(s) 450, 744
MflI RGATCY 2 cut(s) 1039, 1395
MlyI GAGTC 2 cut(s) 1165, 1429
MmeI TCCRAC 3 cut(s) 549, 1221, 1237
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 4 cut(s) 111, 138, 318, 1001
MslI CAYNNNNRTG 4 cut(s) 254, 802, 836, 1188
MspI CCGG 2 cut(s) 84, 954
MspR9I CCNGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
MunI CAATTG 2 cut(s) 450, 744
MvaI CCWGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
MwoI GCNNNNNNNGC 4 cut(s) 898, 975, 1217, 1247
NdeI CATATG 1 cut(s) 799
NdeII GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
NlaIII CATG 6 cut(s) 31, 58, 304, 463, 818, 1383
NlaIV GGNNCC 4 cut(s) 117, 349, 979, 1339
NmuCI GTSAC 2 cut(s) 709, 1365
NspI RCATGY 2 cut(s) 304, 818
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 879
PagI TCATGA 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 724
PflFI GACNNNGTC 1 cut(s) 171
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 7 cut(s) 505, 532, 637, 877, 1086, 1392, 1465
PleI GAGTC 2 cut(s) 1165, 1429
PpsI GAGTC 2 cut(s) 1165, 1429
PpuMI RGGWCCY 2 cut(s) 116, 1282
Psp5II RGGWCCY 2 cut(s) 116, 1282
Psp6I CCWGG 6 cut(s) 180, 546, 892, 910, 1236, 1293
PspGI CCWGG 6 cut(s) 180, 546, 892, 910, 1236, 1293
PspN4I GGNNCC 4 cut(s) 117, 349, 979, 1339
PspPI GGNCC 5 cut(s) 86, 116, 347, 934, 1282
PspPPI RGGWCCY 2 cut(s) 116, 1282
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 2 cut(s) 1039, 1395
PsyI GACNNNGTC 1 cut(s) 171
RsaI GTAC 2 cut(s) 62, 248
RsaNI GTAC 2 cut(s) 61, 247
RseI CAYNNNNRTG 4 cut(s) 254, 802, 836, 1188
SapI GCTCTTC 1 cut(s) 864
SaqAI TTAA 4 cut(s) 111, 138, 318, 1001
SatI GCNGC 7 cut(s) 504, 531, 636, 876, 1085, 1391, 1464
Sau3AI GATC 5 cut(s) 928, 1039, 1180, 1395, 1441
Sau96I GGNCC 5 cut(s) 86, 116, 347, 934, 1282
SchI GAGTC 2 cut(s) 1165, 1429
ScrFI CCNGG 6 cut(s) 182, 548, 894, 912, 1238, 1295
SfaNI GCATC 3 cut(s) 321, 844, 1450
SfcI CTRYAG 2 cut(s) 783, 1450
Sfr274I CTCGAG 1 cut(s) 879
SinI GGWCC 3 cut(s) 86, 116, 1282
SlaI CTCGAG 1 cut(s) 879
SmiMI CAYNNNNRTG 4 cut(s) 254, 802, 836, 1188
SmlI CTYRAG 1 cut(s) 879
SmoI CTYRAG 1 cut(s) 879
SsiI CCGC 3 cut(s) 565, 1109, 1393
SspI AATATT 2 cut(s) 142, 791
SspMI CTAG 3 cut(s) 44, 374, 402
StyD4I CCNGG 6 cut(s) 180, 546, 892, 910, 1236, 1293
StyI CCWWGG 1 cut(s) 1333
TaaI ACNGT 6 cut(s) 70, 256, 584, 666, 1134, 1421
TaiI ACGT 4 cut(s) 172, 679, 754, 1388
TaqI TCGA 2 cut(s) 166, 880
TatI WGTACW 2 cut(s) 60, 246
TfiI GAWTC 1 cut(s) 724
Tru1I TTAA 4 cut(s) 111, 138, 318, 1001
Tru9I TTAA 4 cut(s) 111, 138, 318, 1001
TscAI CASTG 3 cut(s) 259, 300, 1137
TseFI GTSAC 2 cut(s) 709, 1365
TseI GCWGC 7 cut(s) 503, 530, 635, 875, 1084, 1390, 1463
Tsp45I GTSAC 2 cut(s) 709, 1365
TspDTI ATGAA 4 cut(s) 476, 816, 1160, 1259
TspRI CASTG 3 cut(s) 259, 300, 1137
Tth111I GACNNNGTC 1 cut(s) 171
VpaK11BI GGWCC 3 cut(s) 86, 116, 1282
XapI RAATTY 4 cut(s) 441, 463, 990, 1310
XceI RCATGY 2 cut(s) 304, 818
XhoI CTCGAG 1 cut(s) 879
XmiI GTMKAC 2 cut(s) 923, 1423
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 3 cut(s) 44, 374, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.