AT1G21660

Heat shock protein binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004909
Physical Location & Seq
Forward (+)
7605620 .. 7609223
3604 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21660.1

Sequence Viewer

Length: 1572 bp
ATGGACGAGTTTGGTGTTCTAACGGAGCGTTATGGGATTAAGCCACAAGGTAAATCTGCTCCGATGGCTGCTTCTAAGCGTAACCCTAACAACAATGGTCAGAGCTGGAATTTCGGTACAGGCTCCTCTGGTTTAAATGCAAAGTCGACGTCTTTTTCTTCCAATAGCTCTTGGAATAAGGATTCTACTAATGGGTCTTTGTTTGATGGTGACGATATTTTCTTTCCGGCTAACTCTACTAATAGGAAGGTTTCGAATGATTTTGATGTTTTTGCTGGGTTGAACAAATCTTCATCTTCCGGTGATAATAGCAAGTCTTCATTGAACGATGATTTGTTCTTCTCCAATTTTGGGAATCCGGGGAAGACAACTCCTTCTTCAGCTGATGACTTGTTTGGTGGTGTTATTCCTGGGTCTAAAAGCTCTGATAGTGTTAAGAATGATGATTTGTTTGGGTCATTCTCTTCATCGGAGAAGCAATATGCTGCTGCGGTTGATGATTTGTTGGGTTTGGGGAAGAATGAAGCTGGTTATGATGGATTGATACCTGGTTTTGGTGGAAGTACTCAGACGACTAGCAGCAAGACTACACCGTCGAACTTTGATGCTACTGATCCTTTTGTGGTCCTAGAATCAACCACTTCTACTGGTCTGTTCGTGGATCCTCTTGAAGAATTTGCTGCTACAGTCAGCTCTCAGGGGATAAAACCATCAAATACTTCACATACTTCTACAAAACTCAAGCCTCCGCCTAAACCAACACAAAAAGTGAACAGAGGTAAGAGTGTAGGAATGTCTTCAATAGACGAACTTGAAGACTTTGCCATGGGTACTATGCGACGTAGTGCCTCCGCTTCTGACACTGCTAGTAAATATAGAGAAGCTGAAGATGCTGCAACGAAGAATAAACAGTTTGGTGTAGATGACCTTGACTCCTTTTTTAGCGCTCGGTCCAGCAGTGTACCGAAATCACGTACCACTACTGAAACAACTCGCAAGCAAGCGTCCAACGCACCAAAAAAGACCCCTAATGGGGTTTCTAATGCAAAGAAGCCTCCTGCTCCAGCAAATCTGGTGGATGACTTTTCTGCACTTTTTGGAGAGGATCCTATATTTAGACAATTTGAGGAAATCCCAGGGGAAAGCGAAGAACGAAGAAAGGCCAGATGGGATCGTGAACAGAGAACAAAGAGTCGTGTGGCTCAAGCTGTAGCTGATATGAATAACCGTGATCATCAATCTCGGATTGAACAAGAACAGAGAACTAGGATTTCTGAGACTGTTGATACTGAGATAAGGCGTTGGGCAACTGGAAAAGAAGGAAACATGCGTGCGCTGTTATCTTCCTTGCACATCGTACTCTGGCCCGGATGTGGTTGGGAGGCTGTTTCCATAACAGATTTGATCACTTCGTCAGCAGTCAAGAAAGTCTATAGAAAGGCAACACTGTATGTCCATCCCGATAAAGTTCAGCAGAAAGGAGCCACCCTTGAACAAAAGTATATCGCCGAAAAGGTTTTTGACATTTTGAAGGAAGCTTGGAACAAGTTCAACAAGGAGGAGCTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

523

Amino Acids

56.91

Weight (kDa)

6.52

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 592
AatII GACGTC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 146
AciI CCGC 3 cut(s) 491, 749, 852
AclWI GGATC 6 cut(s) 608, 656, 669, 1100, 1113, 1179
AcsI RAATTY 2 cut(s) 109, 674
AcuI CTGAAG 2 cut(s) 363, 906
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 6 cut(s) 118, 565, 832, 963, 976, 1359
AfeI AGCGCT 1 cut(s) 946
AfiI CCNNNNNNNGG 5 cut(s) 351, 554, 1032, 1033, 1373
AgsI TTSAA 9 cut(s) 283, 325, 671, 801, 815, 1250, 1493, 1531, 1552
AjnI CCWGG 3 cut(s) 409, 547, 1135
Alw21I GWGCWC 1 cut(s) 1566
Alw26I GTCTC 1 cut(s) 1271
AlwI GGATC 6 cut(s) 608, 656, 669, 1100, 1113, 1179
Aor51HI AGCGCT 1 cut(s) 946
AoxI GGCC 2 cut(s) 1161, 1364
ApeKI GCWGC 6 cut(s) 68, 485, 488, 579, 680, 893
ApoI RAATTY 2 cut(s) 109, 674
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 2 cut(s) 796, 1547
AspLEI GCGC 2 cut(s) 947, 1336
AspS9I GGNCC 3 cut(s) 625, 951, 1365
AsuC2I CCSGG 2 cut(s) 360, 1368
AsuHPI GGTGA 2 cut(s) 221, 314
AsuII TTCGAA 1 cut(s) 254
AvaII GGWCC 2 cut(s) 625, 951
BamHI GGATCC 2 cut(s) 661, 1105
BanII GRGCYC 1 cut(s) 1566
BbsI GAAGAC 4 cut(s) 309, 371, 789, 822
Bbv12I GWGCWC 1 cut(s) 1566
BbvI GCAGC 6 cut(s) 55, 472, 475, 591, 667, 880
BccI CCATC 6 cut(s) 58, 200, 530, 718, 1161, 1464
BciT130I CCWGG 3 cut(s) 411, 549, 1137
BclI TGATCA 2 cut(s) 1231, 1404
BcnI CCSGG 2 cut(s) 360, 1368
BcoDI GTCTC 1 cut(s) 1271
BfaI CTAG 4 cut(s) 576, 629, 867, 1266
BfmI CTRYAG 3 cut(s) 684, 1209, 1432
BfoI RGCGCY 1 cut(s) 948
BisI GCNGC 6 cut(s) 69, 486, 489, 580, 681, 894
BlsI GCNGC 6 cut(s) 70, 487, 490, 581, 682, 895
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 5 cut(s) 360, 411, 549, 1137, 1368
Bme18I GGWCC 2 cut(s) 625, 951
BmgT120I GGNCC 3 cut(s) 625, 951, 1365
BmiI GGNNCC 4 cut(s) 124, 663, 1107, 1483
BmrFI CCNGG 5 cut(s) 360, 411, 549, 1137, 1368
BmsI GCATC 2 cut(s) 595, 880
BpiI GAAGAC 4 cut(s) 309, 371, 789, 822
BplI GAGNNNNNCTC 1 cut(s) 1550
BpmI CTGGAG 1 cut(s) 1047
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 2 cut(s) 725, 1188
BpuMI CCSGG 2 cut(s) 360, 1368
BsaAI YACGTR 1 cut(s) 974
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 5 cut(s) 359, 410, 825, 1135, 1136
BsaWI WCCGGW 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 5 cut(s) 351, 554, 1032, 1033, 1373
Bse1I ACTGG 2 cut(s) 652, 1315
BseBI CCWGG 3 cut(s) 411, 549, 1137
BseDI CCNNGG 5 cut(s) 359, 410, 825, 1135, 1136
BseGI GGATG 3 cut(s) 1084, 1376, 1456
BseLI CCNNNNNNNGG 5 cut(s) 351, 554, 1032, 1033, 1373
BseMII CTCAG 4 cut(s) 581, 710, 1266, 1281
BseNI ACTGG 2 cut(s) 652, 1315
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 6 cut(s) 55, 472, 475, 591, 667, 880
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 1074
BshFI GGCC 2 cut(s) 1163, 1366
BsiHKAI GWGCWC 1 cut(s) 1566
BsiSI CCGG 4 cut(s) 227, 300, 359, 1368
BslI CCNNNNNNNGG 5 cut(s) 351, 554, 1032, 1033, 1373
BsmAI GTCTC 1 cut(s) 1271
BsnI GGCC 2 cut(s) 1163, 1366
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 1566
Bsp143I GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
Bsp19I CCATGG 1 cut(s) 825
BspACI CCGC 3 cut(s) 491, 749, 852
BspANI GGCC 2 cut(s) 1163, 1366
BspCNI CTCAG 4 cut(s) 580, 709, 1267, 1282
BspLI GGNNCC 4 cut(s) 124, 663, 1107, 1483
BspPI GGATC 6 cut(s) 608, 656, 669, 1100, 1113, 1179
BspT104I TTCGAA 1 cut(s) 254
BsrI ACTGG 2 cut(s) 652, 1315
BssECI CCNNGG 5 cut(s) 359, 410, 825, 1135, 1136
BssMI GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 825
Bst2UI CCWGG 3 cut(s) 411, 549, 1137
Bst4CI ACNGT 6 cut(s) 594, 688, 912, 1229, 1282, 1449
Bst6I CTCTTC 1 cut(s) 469
BstACI GRCGYC 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 974
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 3 cut(s) 998, 1002, 1332
BstDEI CTNAG 5 cut(s) 75, 567, 696, 1275, 1290
BstDSI CCRYGG 1 cut(s) 825
BstF5I GGATG 3 cut(s) 1084, 1376, 1456
BstH2I RGCGCY 1 cut(s) 948
BstHHI GCGC 2 cut(s) 947, 1336
BstKTI GATC 6 cut(s) 616, 664, 1108, 1174, 1234, 1407
BstMAI GTCTC 1 cut(s) 1271
BstMBI GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
BstMWI GCNNNNNNNGC 3 cut(s) 65, 890, 1010
BstNI CCWGG 3 cut(s) 411, 549, 1137
BstNSI RCATGY 1 cut(s) 1330
BstSCI CCNGG 5 cut(s) 358, 409, 547, 1135, 1366
BstSFI CTRYAG 3 cut(s) 684, 1209, 1432
BstV1I GCAGC 6 cut(s) 55, 472, 475, 591, 667, 880
BstV2I GAAGAC 4 cut(s) 309, 371, 789, 822
BstX2I RGATCY 2 cut(s) 661, 1105
BstYI RGATCY 2 cut(s) 661, 1105
BsuRI GGCC 2 cut(s) 1163, 1366
BtgI CCRYGG 1 cut(s) 825
BtsCI GGATG 3 cut(s) 1084, 1376, 1456
BtsI GCAGTG 2 cut(s) 861, 964
BtsIMutI CAGTG 3 cut(s) 861, 964, 1445
Cac8I GCNNGC 3 cut(s) 998, 1002, 1332
CfoI GCGC 2 cut(s) 947, 1336
Cfr13I GGNCC 3 cut(s) 625, 951, 1365
CseI GACGC 1 cut(s) 993
CsiI ACCWGGT 1 cut(s) 547
Csp6I GTAC 6 cut(s) 117, 564, 831, 962, 975, 1358
CspCI CAANNNNNGTGG 2 cut(s) 1056, 1091
CviAII CATG 2 cut(s) 826, 1327
CviQI GTAC 6 cut(s) 117, 564, 831, 962, 975, 1358
DdeI CTNAG 5 cut(s) 75, 567, 696, 1275, 1290
DpnI GATC 6 cut(s) 615, 663, 1107, 1173, 1233, 1406
DpnII GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
DraI TTTAAA 1 cut(s) 135
DrdI GACNNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 469
EarI CTCTTC 1 cut(s) 469
EciI GGCGGA 1 cut(s) 738
Ecl136II GAGCTC 1 cut(s) 1564
Eco130I CCWWGG 1 cut(s) 825
Eco24I GRGCYC 1 cut(s) 1566
Eco47I GGWCC 2 cut(s) 625, 951
Eco47III AGCGCT 1 cut(s) 946
Eco53kI GAGCTC 1 cut(s) 1564
Eco57I CTGAAG 2 cut(s) 363, 906
EcoICRI GAGCTC 1 cut(s) 1564
EcoRII CCWGG 3 cut(s) 409, 547, 1135
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 1 cut(s) 1566
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 2 cut(s) 829, 1330
FatI CATG 2 cut(s) 825, 1326
FbaI TGATCA 2 cut(s) 1231, 1404
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 6 cut(s) 69, 486, 489, 580, 681, 894
FokI GGATG 3 cut(s) 1091, 1383, 1443
FriOI GRGCYC 1 cut(s) 1566
Fsp4HI GCNGC 6 cut(s) 69, 486, 489, 580, 681, 894
FspBI CTAG 4 cut(s) 576, 629, 867, 1266
GlaI GCGC 2 cut(s) 946, 1335
GluI GCNGC 6 cut(s) 69, 486, 489, 580, 681, 894
GsaI CCCAGC 1 cut(s) 279
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 1 cut(s) 948
HaeIII GGCC 2 cut(s) 1163, 1366
HapII CCGG 4 cut(s) 227, 300, 359, 1368
HgaI GACGC 1 cut(s) 993
HhaI GCGC 2 cut(s) 947, 1336
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 829, 1330
Hin6I GCGC 2 cut(s) 945, 1334
HinP1I GCGC 2 cut(s) 945, 1334
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 1536
HinfI GANTC 5 cut(s) 182, 355, 632, 932, 1192
HpaII CCGG 4 cut(s) 227, 300, 359, 1368
HphI GGTGA 2 cut(s) 221, 314
Hpy166II GTNNAC 4 cut(s) 147, 772, 962, 1178
Hpy188I TCNGA 8 cut(s) 63, 102, 427, 472, 570, 859, 1245, 1276
Hpy188III TCNNGA 4 cut(s) 668, 1175, 1423, 1460
Hpy8I GTNNAC 4 cut(s) 147, 772, 962, 1178
Hpy99I CGWCG 3 cut(s) 151, 598, 843
HpyAV CCTTC 4 cut(s) 241, 384, 1313, 1525
HpyCH4III ACNGT 6 cut(s) 594, 688, 912, 1229, 1282, 1449
HpyCH4IV ACGT 3 cut(s) 149, 841, 973
HpyCH4V TGCA 5 cut(s) 140, 896, 1046, 1091, 1351
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 890, 1010
HpyF3I CTNAG 5 cut(s) 75, 567, 696, 1275, 1290
HpySE526I ACGT 3 cut(s) 149, 841, 973
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 829, 1330
HspAI GCGC 2 cut(s) 945, 1334
Ksp22I TGATCA 2 cut(s) 1231, 1404
Kzo9I GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
LmnI GCTCC 6 cut(s) 25, 64, 128, 1066, 1481, 1561
Lsp1109I GCAGC 6 cut(s) 55, 472, 475, 591, 667, 880
LweI GCATC 2 cut(s) 595, 880
MabI ACCWGGT 1 cut(s) 547
MaeI CTAG 4 cut(s) 576, 629, 867, 1266
MaeII ACGT 3 cut(s) 149, 841, 973
MaeIII GTNAC 2 cut(s) 80, 209
MalI GATC 6 cut(s) 615, 663, 1107, 1173, 1233, 1406
MboI GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
MflI RGATCY 2 cut(s) 661, 1105
MhlI GDGCHC 1 cut(s) 1566
MluCI AATT 4 cut(s) 109, 346, 674, 1121
MlyI GAGTC 2 cut(s) 926, 1201
MmeI TCCRAC 1 cut(s) 1032
MroXI GAANNNNTTC 2 cut(s) 796, 1547
MseI TTAA 4 cut(s) 39, 134, 435, 1570
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 4 cut(s) 227, 300, 359, 1368
MspR9I CCNGG 5 cut(s) 360, 411, 549, 1137, 1368
MvaI CCWGG 3 cut(s) 411, 549, 1137
MwoI GCNNNNNNNGC 3 cut(s) 65, 890, 1010
NciI CCSGG 2 cut(s) 360, 1368
NcoI CCATGG 1 cut(s) 825
NdeII GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
NlaIII CATG 2 cut(s) 829, 1330
NlaIV GGNNCC 4 cut(s) 124, 663, 1107, 1483
NmuCI GTSAC 1 cut(s) 209
NspI RCATGY 1 cut(s) 1330
NspV TTCGAA 1 cut(s) 254
PasI CCCWGGG 1 cut(s) 1136
PdmI GAANNNNTTC 2 cut(s) 796, 1547
PfeI GAWTC 3 cut(s) 182, 355, 632
PkrI GCNGC 6 cut(s) 70, 487, 490, 581, 682, 895
PleI GAGTC 2 cut(s) 926, 1200
PpsI GAGTC 2 cut(s) 926, 1200
Ppu21I YACGTR 1 cut(s) 974
Psp124BI GAGCTC 1 cut(s) 1566
Psp6I CCWGG 3 cut(s) 409, 547, 1135
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 3 cut(s) 409, 547, 1135
PspN4I GGNNCC 4 cut(s) 124, 663, 1107, 1483
PspPI GGNCC 3 cut(s) 625, 951, 1365
PsuI RGATCY 2 cut(s) 661, 1105
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 6 cut(s) 118, 565, 832, 963, 976, 1359
RsaNI GTAC 6 cut(s) 117, 564, 831, 962, 975, 1358
SacI GAGCTC 1 cut(s) 1566
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 4 cut(s) 39, 134, 435, 1570
SatI GCNGC 6 cut(s) 69, 486, 489, 580, 681, 894
Sau3AI GATC 6 cut(s) 613, 661, 1105, 1171, 1231, 1404
Sau96I GGNCC 3 cut(s) 625, 951, 1365
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 2 cut(s) 926, 1201
ScrFI CCNGG 5 cut(s) 360, 411, 549, 1137, 1368
SduI GDGCHC 1 cut(s) 1566
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 2 cut(s) 595, 880
SfcI CTRYAG 3 cut(s) 684, 1209, 1432
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 2 cut(s) 625, 951
SmlI CTYRAG 2 cut(s) 740, 1203
SmoI CTYRAG 2 cut(s) 740, 1203
Sse9I AATT 4 cut(s) 109, 346, 674, 1121
SsiI CCGC 3 cut(s) 491, 749, 852
SspMI CTAG 4 cut(s) 576, 629, 867, 1266
SstI GAGCTC 1 cut(s) 1566
StyD4I CCNGG 5 cut(s) 358, 409, 547, 1135, 1366
StyI CCWWGG 1 cut(s) 825
TaaI ACNGT 6 cut(s) 594, 688, 912, 1229, 1282, 1449
TaiI ACGT 3 cut(s) 152, 844, 976
TaqI TCGA 3 cut(s) 146, 254, 596
TaqII GACCGA 1 cut(s) 939
TasI AATT 4 cut(s) 109, 346, 674, 1121
TatI WGTACW 1 cut(s) 563
TfiI GAWTC 3 cut(s) 182, 355, 632
Tru1I TTAA 4 cut(s) 39, 134, 435, 1570
Tru9I TTAA 4 cut(s) 39, 134, 435, 1570
TscAI CASTG 3 cut(s) 868, 964, 1452
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 6 cut(s) 68, 485, 488, 579, 680, 893
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 5 cut(s) 282, 309, 456, 537, 1235
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 3 cut(s) 868, 964, 1452
VpaK11BI GGWCC 2 cut(s) 625, 951
XapI RAATTY 2 cut(s) 109, 674
XceI RCATGY 1 cut(s) 1330
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 2 cut(s) 796, 1547
XspI CTAG 4 cut(s) 576, 629, 867, 1266
ZraI GACGTC 1 cut(s) 150
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.