AT1G21960

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011016
Physical Location & Seq
Forward (+)
7725972 .. 7726586
615 bp
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UTR
Exon/CDS
Intron
AT1G21960.1

Sequence Viewer

Length: 615 bp
ATGGAAGCGGAAATATCCTTTGGCGGCGAAATAGACCTCAAACCGAAATCACAAGATGCTGGATCATTCAAAATCTGCGTAAGTGCCCGAAGATCGGACACATCAATTAGTCTTCCACCAAATTTCGTTCTCTCGTACAATGACTACGAAGGTGTCATATCCTCCTACCCTAATGACTCGAAAAAACTGAATGATTGGTTGTACTCAGCAGGGCTCTCCGAACAATATGTTGACATTGCTAACTCTCAACTCACTGAACGAATCTGTAAGTCGATTTCTATGGTAGACTCTTCAGTTTGTGCTACTTTAGTTTACATGTACTGTAAAGTCATTGTTCCACCTCGGATTGACTCGTTTGATGAGACCACCAATAATATCGAGTTGATTAGACCGGCAAATAAGCTTGTGGTTGACCGTTTGGCAAGGAAAGTATATAATAAGAAGAAGAAATCCAACTCTGACGCGAGCGTGTGCACAATTTGTTTGGAGGAGTTTGAAAAGGGAGAAATAGTGGTGACCTTACCATGTGGCCACGAGTTTGACGATGGATGTATTGGGAAGTGGTTTTTGAAAGACCATGTTTGTCCATTGTGCCGTTTCGAGTTGCCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

204

Amino Acids

22.88

Weight (kDa)

5.18

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 156 - 199 1.7e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 158 - 199 2.1e-14 Ring finger domain
zf-RING_11 PF17123 158 - 185 8.7e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 158 - 198 3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 158 - 198 6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 158 - 196 1.5e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18760 AT1G18770 AT1G18780 AT1G21960 AT2G29840 AT3G13228
fragaria_vesca FvH4_2g27040
malus_domestica MD08G1162100.v1.1
prunus_persica Prupe.1G495200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0295911
rosa_laevigata RLG00000011757
rosa_multiflora Rmu_sc0003139.1_g000008
rosa_roxburghii Rroxscaffold_7G00172060
rosa_rugosa Rorug06G0255000 Rorug06G0255000
rosa_samantha Rh6AG368800 Rh6BG376500 Rh6CG382700 Rh6DG369300
rosa_wichuraiana Rw6G032160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 285
AccII CGCG 1 cut(s) 464
AciI CCGC 2 cut(s) 8, 24
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 529
AcsI RAATTY 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 3 cut(s) 137, 203, 320
AfiI CCNNNNNNNGG 1 cut(s) 94
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 3 cut(s) 70, 497, 571
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 476
Alw26I GTCTC 1 cut(s) 356
Alw44I GTGCAC 1 cut(s) 472
AlwI GGATC 1 cut(s) 70
AoxI GGCC 1 cut(s) 529
ApaLI GTGCAC 1 cut(s) 472
ApoI RAATTY 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 526
BaeGI GKGCMC 2 cut(s) 88, 476
BalI TGGCCA 1 cut(s) 531
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 476
BccI CCATC 1 cut(s) 539
BceAI ACGGC 1 cut(s) 579
BcoDI GTCTC 1 cut(s) 356
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 341
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 391
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 1 cut(s) 554
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 503
BseSI GKGCMC 2 cut(s) 88, 476
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 476
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 356
BsnI GGCC 1 cut(s) 531
Bso31I GGTCTC 1 cut(s) 356
Bsp1286I GDGCHC 3 cut(s) 88, 216, 476
Bsp143I GATC 2 cut(s) 62, 92
BspACI CCGC 2 cut(s) 8, 24
BspANI GGCC 1 cut(s) 531
BspCNI CTCAG 1 cut(s) 218
BspFNI CGCG 1 cut(s) 464
BspPI GGATC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 2 cut(s) 62, 92
BssSI CACGAG 1 cut(s) 533
Bst2BI CACGAG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 323, 416
Bst6I CTCTTC 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 466
BstDEI CTNAG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 514
BstF5I GGATG 1 cut(s) 554
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 2 cut(s) 65, 95
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 2 cut(s) 62, 92
BstNSI RCATGY 1 cut(s) 319
BstPI GGTNACC 1 cut(s) 514
BstSLI GKGCMC 2 cut(s) 88, 476
BstUI CGCG 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 104
BsuRI GGCC 1 cut(s) 531
BtsCI GGATG 1 cut(s) 554
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 391
CseI GACGC 1 cut(s) 470
Csp6I GTAC 3 cut(s) 136, 202, 319
CviAII CATG 3 cut(s) 316, 525, 578
CviJI RGCY 3 cut(s) 214, 403, 531
CviKI_1 RGCY 3 cut(s) 214, 403, 531
CviQI GTAC 3 cut(s) 136, 202, 319
DdeI CTNAG 1 cut(s) 205
DpnI GATC 2 cut(s) 64, 94
DpnII GATC 2 cut(s) 62, 92
EaeI YGGCCR 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 3 cut(s) 319, 528, 581
FaiI YATR 8 cut(s) 158, 228, 281, 317, 433, 435, 526, 579
FatI CATG 3 cut(s) 315, 524, 577
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 1 cut(s) 561
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 25
HaeIII GGCC 1 cut(s) 531
HapII CCGG 1 cut(s) 392
HgaI GACGC 1 cut(s) 470
Hin1II CATG 3 cut(s) 319, 528, 581
HincII GTYRAC 2 cut(s) 232, 412
HindII GTYRAC 2 cut(s) 232, 412
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 176, 261, 287, 350
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 5 cut(s) 232, 286, 313, 412, 474
Hpy188I TCNGA 4 cut(s) 97, 220, 345, 460
Hpy8I GTNNAC 5 cut(s) 232, 286, 313, 412, 474
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 323, 416
HpyCH4V TGCA 1 cut(s) 474
HpyF3I CTNAG 1 cut(s) 205
Hsp92II CATG 3 cut(s) 319, 528, 581
Kzo9I GATC 2 cut(s) 62, 92
LpnPI CCDG 3 cut(s) 45, 195, 405
LweI GCATC 1 cut(s) 46
MaeIII GTNAC 1 cut(s) 514
MalI GATC 2 cut(s) 64, 94
MboI GATC 2 cut(s) 62, 92
MboII GAAGA 5 cut(s) 102, 104, 282, 454, 457
MhlI GDGCHC 3 cut(s) 88, 216, 476
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 3 cut(s) 105, 121, 477
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 3 cut(s) 170, 281, 344
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 4 cut(s) 47, 172, 351, 481
Mox20I TGGCCA 1 cut(s) 531
MscI TGGCCA 1 cut(s) 531
MseI TTAA 1 cut(s) 613
Msp20I TGGCCA 1 cut(s) 531
MspI CCGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 464
NdeII GATC 2 cut(s) 62, 92
NlaIII CATG 3 cut(s) 319, 528, 581
NmuCI GTSAC 1 cut(s) 514
NspI RCATGY 1 cut(s) 319
PciI ACATGT 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 3 cut(s) 170, 281, 344
PpsI GAGTC 3 cut(s) 170, 281, 344
PscI ACATGT 1 cut(s) 315
PspEI GGTNACC 1 cut(s) 514
RsaI GTAC 3 cut(s) 137, 203, 320
RsaNI GTAC 3 cut(s) 136, 202, 319
SaqAI TTAA 1 cut(s) 613
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 2 cut(s) 62, 92
SchI GAGTC 3 cut(s) 170, 281, 344
SduI GDGCHC 3 cut(s) 88, 216, 476
SetI ASST 5 cut(s) 39, 154, 343, 405, 521
SfaNI GCATC 1 cut(s) 46
Sse9I AATT 3 cut(s) 105, 121, 477
SsiI CCGC 2 cut(s) 8, 24
TaaI ACNGT 2 cut(s) 323, 416
TaqI TCGA 4 cut(s) 179, 272, 378, 600
TasI AATT 3 cut(s) 105, 121, 477
TatI WGTACW 2 cut(s) 201, 318
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 1 cut(s) 613
Tru9I TTAA 1 cut(s) 613
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 514
TspRI CASTG 1 cut(s) 259
VneI GTGCAC 1 cut(s) 472
XapI RAATTY 1 cut(s) 121
XceI RCATGY 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.