FvH4_2g27040

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011016
Physical Location & Seq
Reverse (-)
21579611 .. 21580267
657 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27040.t1

Sequence Viewer

Length: 657 bp
ATGGAGACCCTTTACCAATACTCAATCTACCAAGAAGAACAAAACCATGCTTTCCCCAACACCCAGTTGCTCTCCTGCGACTGTTTCATCTTCAACCTTCACTTCACTCATCAGCTCCAACTCTACTACCCCCAACATGTGTTCATACCTTTGAACTCAATCCAAGAATCCTTCTTTGTACCCTGTAACGTGTTCTTTACACATACCCATGACACCATCAACACCATTCTCTCTGGGACAGGTGTGTCCCTGGACTTCATTGAGGCTTTGGTACCTGAAGTTTACAGCTTTGCTTTGGATGTTGCCACTGTGTTCGAAAATGGGGACCCTGATGAGCATAAAGTGGTCCCTTTCAATTTGGGTGTTGTGGCTGTGACGCCTTATGATGATCATGACCAGATTGAGAGGGCTATTTGGGAATCTGGGCAGGTTTTTAATCCGGTTCCGGCCACCAGGTCGTCTATTGCGGCGTTGAAGAAAGTGAAGGTTGAGAATTTGAGCGTGGTGAATGAATGTAGTATTTGTTTGGAGGAGTTTTCAACTGGGTTGGAGGTTATAGGCATGCCCTGCAACCATGTGTATCATAAGGATTGCATTGTTGAGTGGTTGAAGAGGAGTCATTTATGCCCATTGTGCCGGTTTTCGATGCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

219

Amino Acids

24.87

Weight (kDa)

4.87

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 169 - 213 3.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 170 - 213 2.2e-15 Ring finger domain
zf-RING_11 PF17123 171 - 199 7.5e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 171 - 212 1.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 172 - 212 4.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 172 - 210 7e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18760 AT1G18770 AT1G18780 AT1G21960 AT2G29840 AT3G13228
fragaria_vesca FvH4_2g27040
malus_domestica MD08G1162100.v1.1
prunus_persica Prupe.1G495200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0295911
rosa_laevigata RLG00000011757
rosa_multiflora Rmu_sc0003139.1_g000008
rosa_roxburghii Rroxscaffold_7G00172060
rosa_rugosa Rorug06G0255000 Rorug06G0255000
rosa_samantha Rh6AG368800 Rh6BG376500 Rh6CG382700 Rh6DG369300
rosa_wichuraiana Rw6G032160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 418
Acc65I GGTACC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 271
AciI CCGC 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 377
AfaI GTAC 2 cut(s) 180, 273
AflIII ACRYGT 2 cut(s) 136, 189
AgsI TTSAA 6 cut(s) 94, 154, 355, 475, 540, 610
AjnI CCWGG 2 cut(s) 249, 452
AluBI AGCT 2 cut(s) 115, 288
AluI AGCT 2 cut(s) 115, 288
AoxI GGCC 1 cut(s) 447
ApoI RAATTY 1 cut(s) 493
Asp718I GGTACC 1 cut(s) 271
AspS9I GGNCC 2 cut(s) 325, 346
AsuHPI GGTGA 1 cut(s) 517
AsuII TTCGAA 1 cut(s) 315
AvaII GGWCC 2 cut(s) 325, 346
BanI GGYRCC 1 cut(s) 271
BccI CCATC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 251, 454
BclI TGATCA 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 1 cut(s) 468
BlsI GCNGC 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 251, 454
Bme18I GGWCC 2 cut(s) 325, 346
BmgT120I GGNCC 2 cut(s) 325, 346
BmiI GGNNCC 5 cut(s) 273, 326, 327, 348, 444
BmrFI CCNGG 2 cut(s) 251, 454
BmrI ACTGGG 2 cut(s) 58, 552
BmsI GCATC 1 cut(s) 636
BmuI ACTGGG 2 cut(s) 58, 552
Bpu14I TTCGAA 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 636
Bse1I ACTGG 2 cut(s) 64, 547
BseBI CCWGG 2 cut(s) 251, 454
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 304
BseNI ACTGG 2 cut(s) 64, 547
BseRI GAGGAG 2 cut(s) 545, 628
BshFI GGCC 1 cut(s) 449
BshNI GGYRCC 1 cut(s) 271
BsiSI CCGG 3 cut(s) 440, 446, 637
BslFI GGGAC 4 cut(s) 232, 250, 332, 338
BsmFI GGGAC 4 cut(s) 232, 250, 332, 338
BsnI GGCC 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 315
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 1 cut(s) 467
BspANI GGCC 1 cut(s) 449
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 5 cut(s) 273, 326, 327, 348, 444
BspMI ACCTGC 1 cut(s) 418
BspT104I TTCGAA 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 636
BsrI ACTGG 2 cut(s) 64, 547
BssAI RCCGGY 1 cut(s) 636
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 1 cut(s) 377
Bst2UI CCWGG 2 cut(s) 251, 454
Bst4CI ACNGT 2 cut(s) 83, 310
Bst6I CTCTTC 1 cut(s) 605
BstACI GRCGYC 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 567
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 1 cut(s) 563
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 1 cut(s) 391
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 2 cut(s) 567, 633
BstNI CCWGG 2 cut(s) 251, 454
BstNSI RCATGY 2 cut(s) 140, 565
BstSCI CCNGG 2 cut(s) 249, 452
BsuRI GGCC 1 cut(s) 449
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 563
CciI TCATGA 1 cut(s) 391
Cfr10I RCCGGY 1 cut(s) 636
Cfr13I GGNCC 2 cut(s) 325, 346
CseI GACGC 1 cut(s) 385
CsiI ACCWGGT 1 cut(s) 452
Csp6I GTAC 2 cut(s) 179, 272
CviAII CATG 6 cut(s) 47, 137, 209, 392, 562, 575
CviJI RGCY 6 cut(s) 115, 266, 288, 371, 410, 449
CviKI_1 RGCY 6 cut(s) 115, 266, 288, 371, 410, 449
CviQI GTAC 2 cut(s) 179, 272
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
Eco47I GGWCC 2 cut(s) 325, 346
Eco57I CTGAAG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 325
EcoRII CCWGG 2 cut(s) 249, 452
FaeI CATG 6 cut(s) 50, 140, 212, 395, 565, 578
FaqI GGGAC 4 cut(s) 232, 250, 332, 338
FatI CATG 6 cut(s) 46, 136, 208, 391, 561, 574
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 468
GluI GCNGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 449
HapII CCGG 3 cut(s) 440, 446, 637
HgaI GACGC 1 cut(s) 385
Hin1I GRCGYC 1 cut(s) 377
Hin1II CATG 6 cut(s) 50, 140, 212, 395, 565, 578
HinfI GANTC 3 cut(s) 167, 419, 616
HpaII CCGG 3 cut(s) 440, 446, 637
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 1 cut(s) 283
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 3 cut(s) 107, 181, 478
HpyCH4III ACNGT 2 cut(s) 83, 310
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 570, 594
HpyF10VI GCNNNNNNNGC 2 cut(s) 567, 633
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 377
Hsp92II CATG 6 cut(s) 50, 140, 212, 395, 565, 578
KflI GGGWCCC 1 cut(s) 325
KpnI GGTACC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 1 cut(s) 120
LweI GCATC 1 cut(s) 636
MabI ACCWGGT 1 cut(s) 452
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 185, 373
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 4 cut(s) 47, 82, 487, 622
MluCI AATT 2 cut(s) 355, 493
MlyI GAGTC 1 cut(s) 625
MmeI TCCRAC 2 cut(s) 142, 528
MnlI CCTC 5 cut(s) 256, 399, 523, 544, 606
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 207
MspI CCGG 3 cut(s) 440, 446, 637
MspR9I CCNGG 2 cut(s) 251, 454
MvaI CCWGG 2 cut(s) 251, 454
MwoI GCNNNNNNNGC 2 cut(s) 567, 633
NdeII GATC 1 cut(s) 388
NlaIII CATG 6 cut(s) 50, 140, 212, 395, 565, 578
NlaIV GGNNCC 5 cut(s) 273, 326, 327, 348, 444
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 2 cut(s) 140, 565
NspV TTCGAA 1 cut(s) 315
PaeI GCATGC 1 cut(s) 565
PagI TCATGA 1 cut(s) 391
PciI ACATGT 1 cut(s) 136
PfeI GAWTC 2 cut(s) 167, 419
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 1 cut(s) 624
PpsI GAGTC 1 cut(s) 624
PpuMI RGGWCCY 1 cut(s) 325
PscI ACATGT 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 249, 452
PspGI CCWGG 2 cut(s) 249, 452
PspN4I GGNNCC 5 cut(s) 273, 326, 327, 348, 444
PspPI GGNCC 2 cut(s) 325, 346
PspPPI RGGWCCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 180, 273
RsaNI GTAC 2 cut(s) 179, 272
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 2 cut(s) 325, 346
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 2 cut(s) 251, 454
SexAI ACCWGGT 1 cut(s) 452
SfaNI GCATC 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 315
SinI GGWCC 2 cut(s) 325, 346
SmiMI CAYNNNNRTG 1 cut(s) 207
SphI GCATGC 1 cut(s) 565
Sse9I AATT 2 cut(s) 355, 493
SsiI CCGC 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 249, 452
TaaI ACNGT 2 cut(s) 83, 310
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 315, 644
TasI AATT 2 cut(s) 355, 493
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 2 cut(s) 167, 419
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 373
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 4 cut(s) 76, 133, 247, 525
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 325, 346
XapI RAATTY 1 cut(s) 493
XceI RCATGY 2 cut(s) 140, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.